CN112400808A - Breeding method and application of panonychus citri sensitive strain - Google Patents
Breeding method and application of panonychus citri sensitive strain Download PDFInfo
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- CN112400808A CN112400808A CN202011161287.8A CN202011161287A CN112400808A CN 112400808 A CN112400808 A CN 112400808A CN 202011161287 A CN202011161287 A CN 202011161287A CN 112400808 A CN112400808 A CN 112400808A
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- filter paper
- panonychus citri
- leaves
- mites
- sponge
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- 241000488581 Panonychus citri Species 0.000 title claims abstract description 31
- 238000009395 breeding Methods 0.000 title claims description 15
- 238000000034 method Methods 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims description 27
- 241000238876 Acari Species 0.000 claims description 18
- 229920000742 Cotton Polymers 0.000 claims description 12
- 230000001488 breeding effect Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241001454295 Tetranychidae Species 0.000 claims description 8
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 230000002265 prevention Effects 0.000 claims description 6
- 241000207199 Citrus Species 0.000 claims description 5
- 235000020971 citrus fruits Nutrition 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims 2
- 239000000126 substance Substances 0.000 abstract description 4
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000002035 prolonged effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000010019 sublethal effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical group Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000851181 Eutetranychus orientalis Species 0.000 description 1
- 241001454294 Tetranychus Species 0.000 description 1
- 241001454293 Tetranychus urticae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of biological control, and particularly discloses a method for feeding panonychus citri sensitive strains and application thereof. The invention provides a method for feeding panonychus citri single-head, which is applied to a life surface technology, so that the method is effectively utilized to carry out biological observation on the panonychus citri single-head, and a theoretical basis is provided for selecting a proper chemical method to prevent and control the panonychus citri.
Description
Technical Field
The invention relates to the field of biological control, and particularly relates to a feeding method and application of panonychus citri sensitive strains.
Background
At present, for panonychus citri which is a tetranychidae harmful to economic crops, various methods are combined for controlling the panonychus citri, but chemical control is still mainly used. The large, continuous and wide use of chemical pesticides, and the characteristics of small mite body, multiple generations, high breeding speed, short development period and the like inevitably lead to more and more serious drug resistance, and seriously threaten the effectiveness of chemical control in production. The sub-lethal dose of the insecticide (mite) causes the environmental condition that the field pests (mites) grow rampant, the sub-lethal dose of the insecticide (mite) causes the change of biology, ecology, behaviors, reproductive capacity and the like of insects (mites), so as to cause the change of insect (mite) population, and in order to find out the influence of the sub-lethal dose of the insecticide (mite) on the survival, growth and development and reproduction of the whole citrus mites, the sub-lethal effect of a specific insecticide (mite) on specific spider mites is explained by adopting a life-form technology.
When the sub-lethal effect of a specific insecticide (mite) agent on specific tetranychus urticae is researched, 1) a stable mite source of the panonychus citri sensitive strain is needed, domestic modes mostly concentrate on cultivation in a feeding box or potted plant, the traditional method has large space occupancy rate, consumes time and labor, and is difficult to culture in a large scale when the sample demand is large; 2) the method is characterized in that a study object needs to be picked and fed at a single end for biological observation, the domestic mode mostly focuses on a leaf disk feeding observation method, a traditional leaf disk (placed in a culture dish with sponge) is covered with a black plastic film, plant leaves are placed, and soaked cotton is surrounded by the plant leaves; the method has the advantages that the material consumption is large, the space occupancy rate is high, batch operation required by experiments is difficult to guarantee during manufacturing, and meanwhile, due to the fact that the number of experimental samples is too large, observation times are frequent, and the method is extremely inconvenient during transferring of leaf discs. For the above reasons, leaf discs made by conventional methods on a large scale are rarely seen at present.
Based on the consideration, the invention provides the method for breeding panonychus citri, which can not only quickly expand the population, but also prevent tetranychidae from escaping, is simple and easy to manufacture, saves space, can be operated in batches and is convenient to transfer and observe.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the method for breeding the panonychus citri sensitive strain, which can not only quickly propagate the population, but also prevent mites from escaping.
The invention also aims to provide a panonychus citri feeding method applied to the life surface technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a method for breeding panonychus citri sensitive strains, which comprises the following steps:
1. and (3) sensitive strain population establishment:
comprises the following steps: the box, the orange twigs and the cotton which are made of acrylic materials and provided with 5 holes after being modified
Drawing a square by 10 cm-10 cm acrylic box filled with water, chiseling 1 hole at each vertex and center, selecting and picking 5 citrus twigs, and washing with clear water;
the method comprises the steps of inserting twigs into 5 holes, wrapping cotton at the tail ends of the twigs for fixing plants and preventing mites from escaping, selecting 20 pieces of male and female adult mites obtained by sampling from leaves, and utilizing the characteristics of more generations and fast propagation of panonychus citri to enable the panonychus citri to quickly infect among 5 twigs for multi-generation screening to establish a sensitive strain mite source.
2. Single-head cultivation of mites of the same age:
comprises a glass culture dish with a square sponge, square filter paper arranged above the sponge, plant leaves for feeding panonychus citri, and double-shaped filter paper covered on the leaves;
1) early preparation: the square filter paper has the same shape and size as the sponge; the diameter of the plant leaf is divided according to the length of the side of the square filter paper, and the length of the inner side and the outer side of the paper with the same length as the diameter of the plant leaf is specified according to the diameter of the plant leaf;
2) covering a layer of square filter paper with the same size on the sponge, and placing 16 round plant leaves punched by a puncher on the square filter paper; placing 16 leaves according to 4-by-4 (row/column), and covering the plant leaves (2) which are punched by 16 round punches with the clip-type filter paper.
3) Soaking the upper layer (the clip-shaped filter paper) and the lower layer (the square filter paper) by using a syringe with a needle head containing clear water, attaching the upper layer (the clip-shaped filter paper) and the lower layer (the square filter paper) to prevent the spider mites from escaping, observing the spider mites regularly by using a body type microscope, counting the escaping condition of an experimental sample, and checking the escaping prevention rate of the method.
The invention has the advantages that:
1) according to the method for feeding panonychus citri, the morphological identification of each mite state of the panonychus citri is added, so that the growth and development of each stage of the panonychus citri can be controlled, a lower oviposition amount and a longer life history are obtained, the generation period is prolonged, the propagation rate is slowed down, and a theoretical basis is provided for the prevention and treatment of the panonychus citri.
2) Compared with the traditional breeding box culture or potted plant cultivation, the panonychus citri sensitive strain breeding method provided by the invention overcomes the problems of large space occupancy rate, time and labor consumption and large-scale culture when the sample demand is large.
3) According to the method for feeding panonychus citri individuals, one dish of multiple cultures are adopted, the problem of space occupancy rate of the traditional leaf disc manufacturing method is solved, batch operation can be realized, the requirement of large-scale observation of leaf disc manufacturing of experimental samples is met, the water retention performance of the individual culture device for the leaves is excellent, and accidental damage of the experimental samples caused by frequent blade replacement is effectively avoided; the invention carries out timing observation through a body type microscope, counts the escape condition of an experimental sample and tests the escape prevention rate of the method. The escape prevention rate of 100 percent by using the method can be obtained, and the plant moisturizing cycle lasts for one week.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below.
FIG. 1 is a schematic diagram of: schematic diagram of overall structure effect of propagation breeding box manufactured by using method of the invention
1-square acrylic box with 5 holes, 2-water injection holes, 3-citrus twigs and 4-cotton
FIG. 2 is a diagram of: schematic diagram of the overall structure effect of the individual feeding device manufactured by the method
FIG. 3 is a morphological diagram of each growth phase under a microscope with a posterior body of a leaf disk manufactured by the method of the present invention
In the figure, a, b, young, c, d, female, e, male, etc. mites
Detailed Description
The experimental methods in the following examples are conventional methods unless otherwise indicated, and materials to which the present invention relates are all commonly available and commercially available. The invention is further described below with reference to the following figures and examples:
example 1: propagation device assembly
Drawing a square by 10 cm-10 cm acrylic box filled with water, chiseling 1 hole at each vertex and center, selecting and picking 5 citrus twigs, and washing with clear water; the method comprises the steps of inserting twigs into 5 holes, wrapping cotton at the tail ends of the twigs for fixing plants and preventing mites from escaping, selecting 20 pieces of male and female adult mites obtained by sampling from leaves, and utilizing the characteristics of more generations and fast propagation of panonychus citri to enable the panonychus citri to quickly infect among 5 twigs for multi-generation screening to establish a sensitive strain mite source.
Example 2: manufacturing of individual breeding device
Firstly, putting a cut square sponge with the side length of 10 x 10cm in a glass culture dish with the diameter of 15cm, soaking the square sponge with clean water, then spreading a cut square filter paper with the side length of 10 x 10cm above the sponge, exhausting bubbles, enabling the filter paper to be attached to the sponge, putting plant leaves with the diameter of 2.5cm and punched by a puncher according to 4 x 4 (rows/columns), finally covering the cut back filter paper (the outer side length is 2.5 x 2.5cm, the inner side length is 1.5 x 1.5cm) on the plant leaves, putting 2 layers of back filter paper on each leaf, soaking the upper and lower layers of filter paper by using an injector with a needle head, and manufacturing the filter paper for experimental feeding and insect observation. In order to detect the effect after the method is used, observation is carried out every 8 hours, the escape number of experimental samples is counted, the escape rate is calculated, the escape rate is 0 after 24 hours, the effect that the leaf disc manufactured by the method is high in observation escape prevention index is shown to be high, the wilting degree of the leaves of the plant after 7 days is low, the effect that the leaf disc manufactured by the method is good in plant water retention performance is shown to be observed, the leaf replacement period is prolonged, the leaf replacement frequency is reduced, but in order to avoid experimental result errors caused by the fact that the leaves are not fresh, the fresh leaves need to be replaced regularly, and the replacement period can be properly prolonged and the replacement frequency can be reduced.
Example 3 growth and development of Tetranychus citriodorus at 28 ℃
Egg: the development period is 4-5 days, and the color of the product gradually fades after the product is fresh red at first birth. The egg has a vertical small handle, and the handle end has filaments which scatter and extend to the periphery and are attached to the egg laying position.
Young mites: the development period is 1.5-2d, the body length is 0.2 mm, and the color is light red when the eggs are initially hatched, and the number of the eggs is 3.
If mites: the development period is 2-3d, the shape and color are similar to those of adult mites, but the individual is slightly small, and the number of pairs of adult mites is 4.
And (3) forming mites: the development period is 11-15 days, 4 pairs of feet, the female adult mite body is oval, the back surface is raised, the side surface looks like a hemisphere, and the color is deep red to dark red; the male adult mites are slightly rhomboid, slightly smaller in size and sharper in end.
Surface growth and development of panonychus citri under 128 deg.C
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent modifications made by the present invention and the contents of the drawings or directly or indirectly applied to the related technical fields are included in the scope of the present invention.
Claims (5)
1. A method for breeding panonychus citri sensitive strains, which is characterized by comprising the following steps:
1. breeding the panonychus citri sensitive strain population:
comprises a box made of acrylic materials with 5 holes, citrus twigs and cotton;
1) early preparation: chiseling 5 holes with the diameter of 0.5cm on the acrylic box by using a five-point method, and preparing 5 citrus twigs and 5 clusters of cotton;
2) respectively surrounding the roots of the 5 groups of cotton with the cotton buds, inserting the cotton buds into 5 holes of the acrylic box filled with water to prevent mites from escaping and play a role in fixing plants, facilitating the 5 cotton buds to be contacted with each other and facilitating the mites to be infected with each other, and picking fresh cotton buds for a period of time to replace citrus branches infected with the mites previously; by the repeated steps, stable panonychus citri sensitive strain mite sources are screened and established for multiple generations;
2. the method is applied to experimental individual single-head feeding of the life-form technology:
comprises a glass culture dish with a square sponge, square filter paper arranged above the sponge, plant leaves for feeding spider mites, and double-shaped filter paper covering the leaves;
1) early preparation: the square filter paper is the same as the sponge in shape and size; the diameter of the plant leaf is divided according to the length of the side of the square filter paper, and the length of the inner side and the outer side of the paper with the same length as the diameter of the plant leaf is specified according to the diameter of the plant leaf;
2) covering a layer of square filter paper with the same size on the sponge, and placing 16 round plant leaves punched by a puncher on the square filter paper; placing 16 leaves according to 4-by-4 rows/columns, and covering the plant leaves which are punched by 16 round punches with the clip-shaped filter paper;
3) soaking the upper layer of paper with a double-end filter paper and the lower layer of square filter paper by using a syringe with a needle head containing clear water, attaching the paper with the double-end filter paper to prevent spider mites from escaping, after a single individual rearing device is manufactured, picking 16 leaves in the rearing device into the same mite state and -stage worm bodies, regularly observing the worm bodies by using a body type microscope, counting the escaping condition of an experimental sample, and checking the anti-escaping rate.
2. Use of panonychus citri obtained by the process according to claim 1 in the life-sheet technique, characterized in that it comprises the following steps:
1) establishing a panonychus citri sensitive strain population to obtain a stable mite source required by an experiment;
2) breeding a single individual, and finally performing biological observation on the insects in the same mite state age;
3) the specific manufacturing method of the individual breeding device comprises the following steps: covering a layer of square filter paper with the same size on the sponge, and placing 16 round plant leaves which are punched by a puncher and used for feeding spider mites on the square filter paper; placing 16 leaves according to 4-by-4 rows/columns, and covering the plant leaves which are punched by 16 round punches with the clip-shaped filter paper;
4) and (4) regularly observing by using a body type microscope, counting the escape condition of the experimental sample, and checking the escape prevention rate of the invention.
3. Use according to claim 2, characterized in that in step 1): the breeding container is an acrylic box with 5 holes.
4. Use according to claim 2, characterized in that in step 3): the individual breeders are leaf disks.
5. Use according to claim 2, characterized in that in step 4): observing by a microscope, counting the escape number every 8h, and replacing the plant leaves every 7 d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011161287.8A CN112400808A (en) | 2020-10-27 | 2020-10-27 | Breeding method and application of panonychus citri sensitive strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011161287.8A CN112400808A (en) | 2020-10-27 | 2020-10-27 | Breeding method and application of panonychus citri sensitive strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112400808A true CN112400808A (en) | 2021-02-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011161287.8A Withdrawn CN112400808A (en) | 2020-10-27 | 2020-10-27 | Breeding method and application of panonychus citri sensitive strain |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810155A (en) * | 2010-04-27 | 2010-08-25 | 南京农业大学 | Tetranychina harti feeder |
| CN107279069A (en) * | 2017-07-24 | 2017-10-24 | 广西南亚热带农业科学研究所 | A kind of method of efficient breeding cassava Tetranychus cinnabarinus |
| CN107494452A (en) * | 2017-09-19 | 2017-12-22 | 南昌大学 | A kind of quick propagation method of Tetranychus cinnabarinus |
| CN209931255U (en) * | 2019-03-12 | 2020-01-14 | 广东省农业科学院植物保护研究所 | Indoor isolation feeder for spider mite population subculture feeding and breeding |
-
2020
- 2020-10-27 CN CN202011161287.8A patent/CN112400808A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810155A (en) * | 2010-04-27 | 2010-08-25 | 南京农业大学 | Tetranychina harti feeder |
| CN107279069A (en) * | 2017-07-24 | 2017-10-24 | 广西南亚热带农业科学研究所 | A kind of method of efficient breeding cassava Tetranychus cinnabarinus |
| CN107494452A (en) * | 2017-09-19 | 2017-12-22 | 南昌大学 | A kind of quick propagation method of Tetranychus cinnabarinus |
| CN209931255U (en) * | 2019-03-12 | 2020-01-14 | 广东省农业科学院植物保护研究所 | Indoor isolation feeder for spider mite population subculture feeding and breeding |
Non-Patent Citations (2)
| Title |
|---|
| 辛天蓉等: "亚致死浓度除虫脲对朱砂叶螨生长和繁殖的影响", 《应用昆虫学报》 * |
| 龚舒: "月季二斑叶螨实验种群生态特性及防治技术研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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Application publication date: 20210226 |