CN206453020U - A kind of device for being applicable planthopper isolated rearing and Observation of biological characteristics - Google Patents

Abstract

The utility model relates to a device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, which comprises a transparent tube cover, a plastic cup and a bottom box; the transparent tube cover is composed of a plurality of single transparent tube covers bundled; each The upper opening of a transparent tube cover is wrapped by breathable gauze, and the lower opening is stuffed with cotton; the plastic cup is a transparent structure, its bottom is closed, and the top is open; the plastic cup contains nutrient solution accounting for one-third of its volume ;The bottom box is a sealed and light-proof structure; the top surface of the bottom box is evenly opened with a placement hole for placing plastic cups; the diameter of the placement hole is larger than the bottom diameter of the plastic cup and smaller than the top diameter of the plastic cup; the transparent cover after binding Place in a plastic cup and place it in the placement hole along with the plastic cup. The utility model has a simple and reasonable structural design, not only rice at the seedling stage but also a single rice plant at the tillering stage can be used to raise planthoppers, and it is helpful to obtain various rice materials under the stress of feeding by rice planthoppers at different times.

Description

A device suitable for isolated rearing of rice planthopper and observation of biological characteristics

technical field

The utility model belongs to the technical research device field of insect feeding, insect biological observation, induced resistance mechanism research and insect-host interaction influence relationship in the direction of plant protection, and specifically relates to a kind of isolation feeding and biological characteristics suitable for rice planthoppers observation device.

Background technique

Rice is one of the three major grain crops. Rice planthopper (BPH, white-backed planthopper, and SBPH) is an important pest that harms rice in various rice regions such as my country and Southeast Asia. For a long time, research on rice planthopper at home and abroad has concentrated The breeding and population establishment of rice planthopper is an important basis for related research on the law of occurrence and control technology.

The devices and methods for raising rice planthoppers indoors vary in different places and different laboratories due to various factors. At present, the cost of artificial feed is high, the operation is troublesome, and the survival rate of rice planthoppers is low and the fecundity is reduced. Therefore, the main feed for rice planthoppers is rice. According to different uses and different purposes, rice of different periods is selected, such as buds, seedlings, and tillers, and the devices and methods used are also different.

At present, rice planthoppers are reared in groups indoors, and rice buds are mainly cultivated in insect cages made of gauze or nylon nets. This method is difficult to control the age of planthoppers, and it is not convenient to transfer them. It is suitable for Large-scale and extensive seed conservation breeding; soil cultivation of rice seedlings in beakers, and group rearing of rice planthoppers have achieved good results in controlling the age of planthoppers (Ren Chunmei, Cheng Zhaobang, Zhou Yijun, etc., application number: 201410298198.6), However, this method is cumbersome and requires a lot of work. Planthoppers often escape when rice seedlings are replaced, and soil-cultured rice is unhygienic, prone to mold, and it is inconvenient to observe the biological characteristics of planthoppers; Conical observation container, carry out group breeding of planthoppers with rice buds (Li Xiangyong, Yin Yanqiong, Chen Aidong, etc., application number 201610142820.3), this method is raised with rice seedlings, needs to change seedlings frequently, and because the roots of rice are inserted into the sponge , it is inconvenient to replace the rice, and the eggs on the seedlings will be missed, and the sponge pads separated up and down can easily make the young planthopper nymphs escape or get trapped and die in the hole, so it is more complicated to raise a large number of planthoppers.

The isolation and rearing of rice planthoppers indoors mainly adopts single-head, single-pair and other methods. Liu Yudi et al. put rice seedlings or tillers into a small flat-bottomed test tube (15 cm long and 2.5 cm in diameter) filled with nutrient solution at the bottom to raise them (Liu Yudi, Hou Maolin, application number: 201520084390.5). head or a single pair of planthoppers, but it is inconvenient to replace the liquid soaked in the lower part of the rice root because there is only one opening, and the planthoppers are easy to escape when the rice is replaced, and the stability of the device is not strong. The material of the test tube is glass, so pay attention It is easy to break, and the sponges that seal both ends are easy to make the young planthopper nymphs escape or get trapped in the hole and die; Li Kailong et al. put the hollow cylinder with both ends open on the plastic cup filled with solid medium to raise planthoppers (Li Kailong , Tang Yaohua, Wang Weixia, etc., application number: 201520528370.2), the device did not fix the hollow cylinder, and did not isolate the hollow cylinder from the plastic cup, which easily caused the death of planthopper nymphs; The two ends of the double-layer gauze are passed through the glass tube, and then the whole is erected in a water basin to keep moisture to raise planthoppers (Feng Bingcan, Huang Ciwei, Wang Huandi, etc. The influence of temperature on the population growth of white-backed planthoppers[J]. Acta Entomology, 1985 (4) 390-397.) Because the material of the tube is glass, it is inconvenient to move and fragile. The lower opening is wrapped with gauze to keep moisture, which is not conducive to plant growth and planthoppers are easy to fall on the water surface. And pipe is also not easy to observe together being placed in water basin.

Contents of the invention

In view of the above problems, the utility model proposes a simple and reasonable structure design, convenient use, easy transfer, simple self-made, clear observation, can not only raise planthoppers individually, but also group breeding, and can also observe the physiological behavior of planthoppers s installation. Not only rice at the seedling stage can be used, but also one or more generations of planthoppers in various stages can be continuously raised with a single plant of rice at the tillering stage, so as to prolong the breeding time of generations, facilitate the observation of the biological characteristics of planthoppers, and help to obtain planthoppers affected by planthoppers. Research on various rice materials under feeding stress, the mechanism of rice resistance to planthopper induction, and the relationship between interaction and influence, etc.

The technical scheme of the utility model is as follows:

The above-mentioned device suitable for isolated breeding of rice planthoppers and observation of biological characteristics includes a transparent tube cover, a plastic cup and a bottom box; the transparent tube cover is composed of more than one single transparent tube cover; each of the The transparent tube covers are hollow cylindrical tubes with two openings, the upper opening is wrapped by a breathable gauze, and the lower opening is stuffed with cotton; the stems of rice plants are inserted into the lower opening of the transparent tube cover and the The cotton is covered, and the cotton fills the gap at the lower opening of the transparent tube cover; the plastic cup is a transparent structure with a closed bottom and an open top with a diameter larger than that of the bound transparent tube cover. The largest diameter; the rice nutrient solution that accounts for one-third of its volume is filled in the plastic cup; the bottom box is an airtight and light-proof structure, and its height is less than the height of the plastic cup and greater than the height of the plastic cup half; the top surface of the bottom box is uniformly provided with a placement hole for placing the plastic cup; the diameter of the placement hole is larger than the bottom diameter of the plastic cup and smaller than the top diameter of the plastic cup; The transparent tube cover is placed in the plastic cup and placed in the placement hole together with the plastic cup.

The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, wherein: a single transparent tube cover is made of acrylic plastic, with a length of 15-30 cm and a diameter of 2-4 cm.

The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, wherein: the cotton fills the gap between the lower opening and the upper 2-4 cm of the transparent tube cover; the mesh density of the breathable gauze is 80 ~100 mesh.

The device suitable for isolated rearing of rice planthopper and observation of biological characteristics, wherein: the height of the plastic cup is 15 cm, the diameter of the bottom is 5-6 cm, and the diameter of the top is 8-10 cm.

The device suitable for isolated rearing of rice planthopper and observation of biological characteristics, wherein: the material of the plastic cup is PET or PP transparent plastic.

The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, wherein: the height of the bottom box is 8-14 cm; the diameter of the placement hole is 6-8 cm.

The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, wherein: the transparent tube cover is composed of more than one transparent tube cover monomers bound by rubber bands; The breathable gauze is wrapped and fixed with rubber bands.

Beneficial effect:

The utility model has the advantages of simple and reasonable structural design, convenient use, convenient transfer, easy self-control, and clear observation. It can not only raise planthoppers on a single head, but also basically meet the requirements of group breeding of planthoppers. It has the advantage of controlling the age of planthoppers, keeps them basically consistent, and meets the requirements of carrying out experiments related to planthoppers. At the same time, it also has the characteristics of being clean and easy to replace rice. The transparent tube cover has strong plasticity, and can be designed with different lengths and diameters according to the needs to fit rice stems of various sizes, so that it is convenient to obtain rice materials at different growth stages under the feeding stress of planthoppers; fill the transparent tube with cotton The gap between the opening under the tube cover and the rice stem prevents planthoppers from falling into the cup and dies. Compared with sponges, it has the advantage of preventing planthopper nymphs from being trapped in the sponge gap and reducing their escape rate, and it can also play a role in rice plants. To stabilize the effect; the material of the transparent tube cover is acrylic plastic, which has the characteristics of durability, transparency, non-toxicity, stability, strong plasticity, and lightness; the combination of multiple transparent tube covers and plastic cups is placed in a rectangular parallelepiped bottom box, which can Simultaneously transfer and place several, which is convenient for the experiment and can also help to expand the number of experimental samples; the bottom box is made of airtight and opaque carton, which can shade the plastic cup, which can prevent the generation of algae in the nutrient solution and avoid other Photosynthesis can also play a certain stabilizing effect on the main body of the device.

It also has the following advantages:

(1) The utility model has the advantages of being strong, easy to carry, not easy to be damaged, long in use, and convenient to replace rice; the transparent tube cover is designed to open at both ends, and the rice stem is covered with cotton to fix the rice and separate the flying rice. The function of lice can prevent planthoppers from falling on the liquid at the root of rice, reduce the death rate and escape rate of planthoppers, and also help prevent planthoppers from escaping when rice is replaced; whether it is a single head in the laboratory, group breeding or as a field The storage and transfer devices for collecting planthoppers are all competent.

(2) The utility model is bundled with a plurality of transparent tube covers, which can more conveniently observe the space for a plurality of isolation breeding planthoppers, and it is also easy to replace the nutrient solution of rice roots by using plastic cups and bottom boxes to extend the use of rice time, reduce the number of rice replacements, and can also raise single-head and single-pair planthoppers, which is convenient for the development of experiments with the biological characteristics of planthoppers, and can meet the separation and breeding of more planthopper generations for a longer period of time. After testing, using the utility model to raise planthoppers, the main stem of rice at the tillering stage can still maintain good vitality after at least one planthopper generation, which reduces the frequency of replacing rice, saves labor, and is beneficial for planthoppers to eat rice. Experiments with various lengths of time are convenient for the determination of physiological indicators under different conditions, and it is also easy to obtain rice materials at different growth stages under the feeding stress of planthoppers, and avoid being limited to breeding with rice buds and rice seedlings.

(3) After the planthoppers feed, the vitality of the rice will gradually weaken. When the rice needs to be replaced, you only need to take out the upper part of the device, put it on a horizontal surface, and press the cotton surrounding the rice stem with your hands. , Gently pull out the rice with poor vitality, the planthoppers will safely fall into the transparent tube cover, and then wrap the new rice with cotton and put it in the transparent tube cover. If you have reared younger planthoppers or raised female adults of planthoppers that are laying eggs and you do not want to take out the rice but the original plant grows weak and you have to replace the rice, you only need to mix the rice plants with the cotton Surround the exposed part, discard the cut off part with roots, put the removed upper part on the horizontal surface, take out the cotton to wrap the new rice and put it in a transparent tube cover, then there are two batches of rice with different growth Allowing planthoppers to transition reduces the escape rate of planthoppers when changing rice. Then use this device to continue to raise planthoppers, and take out the previous rice until the next time rice is changed. If you need to transfer planthoppers, you only need to put the tube cover on a horizontal plane, uncover the gauze, and suck it with an insect sucker.

Description of drawings

Fig. 1 is a structural schematic diagram of a device suitable for isolated rearing of rice planthoppers and observation of biological characteristics of the utility model.

detailed description

As shown in FIG. 1 , the utility model is suitable for isolated breeding of rice planthoppers and observation of biological characteristics, including a transparent tube cover 1 , a plastic cup 2 and a bottom box 3 .

This transparent cylinder cover 1 is the hollow cylinder tube that both ends are all open, can be 15～30cm according to the different setting length of paddy growth stage, variety and plant height, and aperture is set as 2～4cm, and length and aperture can be adjusted generally. The difference is 20cm and 2.5cm. Wherein, the upper opening of the transparent tube cover 1 is covered with a breathable gauze 11, and the lower opening is plugged with cotton 12; the breathable gauze 11 is suitable for 80-100 mesh (it can prevent smaller planthopper nymphs from escaping), and the size is larger than The diameter of the upper opening of the transparent cylinder cover 1 is just enough to wrap it up. After wrapping the upper opening of the transparent cylinder cover 1, it is fixed with a rubber band 13. It is better to select a softer air-permeable gauze 11 to facilitate wrapping. The material of the transparent cylinder cover 1 is preferably acrylic plastic (the main component is polymethyl methacrylate, namely PMMA, also known as plexiglass). If there is no condition, it can also be replaced by glass or quartz tube. The stem of the rice plant 4 is stuffed into the transparent tube cover 1 from the lower opening to the upper 2-4 cm and covered with cotton 12, and the cotton 12 fills the gap between the lower opening of the transparent tube cover 1 and the upper 2-4 cm to avoid Planthoppers fall into the cup and escape or die to fix and isolate the rice; the rice plant 4 is used as planthoppers to feed, and rice at different growth stages such as seedling stage and tillering stage are selected for experiments and feeding according to different purposes. The transparent tube cover 1 is composed of a plurality of transparent tube cover monomers bound by rubber bands 13 (in this embodiment, four transparent tube cover monomers are bound by rubber bands 13). Different lengths and calibers are designed to be suitable for loading rice stems of various sizes, so that it is convenient to obtain rice materials at different growth stages under the feeding stress of planthoppers.

The plastic cup 2 is used to hold the rice nutrient solution and fixes the combined transparent tube cover 1; the plastic cup 2 is a transparent structure with an open top and a closed bottom, with a height of about 15 cm and a bottom diameter of 5-5 cm. 6cm, the top caliber is 8-10cm and slightly larger than the maximum diameter of the combined transparent tube cover 1, so that a certain included angle can be formed so that the combined transparent tube cover 1 can be buckled stably in the plastic cup 2; wherein, the plastic cup The material of 2 is PET or PP transparent plastic, which holds water or nutrient solution accounting for one-third of its volume when in use, and the nutrient solution is preferably a nutrient solution suitable for the growth of the plant, such as Kimura B nutrient solution It is used to cultivate rice and is replaced regularly, usually every 3 days or so.

The bottom box 3 has a rectangular parallelepiped structure and is made of an airtight and light-tight carton, which can shade the plastic cups and avoid the growth of algae in the nutrient solution. The length and width of this bottom box 3 are indefinite, adjust according to the plastic cup 2 quantity that needs to place; ～14cm; the top surface of the bottom box 3 is provided with a plurality of placement holes 31 for placing plastic cups 2, which can transfer and place multiple plastic cups 2 and fix them at the same time, so as to facilitate the experiment; the number of placement holes 31 Determined according to requirements, and the diameter is 6-8 cm. The diameter of the placement hole 31 is larger than the bottom diameter of the plastic cup 2 and smaller than the upper diameter of the plastic cup 2, so as to stabilize the plastic cup 2 . The bundled transparent tube cover 1 is placed in the plastic cup 2 and placed in the placement hole 31 together with the plastic cup 2, and is used to raise planthoppers or obtain rice materials that are threatened by planthopper feeding.

The utility model has the advantages of simple and reasonable structural design, convenient use, convenient transfer, easy self-control, clear observation, and can not only raise planthoppers individually, but also basically meet the requirements of group breeding of planthoppers.

Claims (7)

1. A device suitable for isolated breeding of rice planthoppers and observation of biological characteristics, characterized in that: the device includes a transparent tube cover, a plastic cup and a bottom box; The transparent tube cover is composed of more than one single transparent tube cover bundled; each of the transparent tube covers is a hollow cylindrical tube with two openings, the upper opening is wrapped by a breathable gauze, and the lower opening is filled with Cotton; the stem of the rice plant is inserted into the lower opening of the transparent tube cover and covered by the cotton, and the cotton fills the gap at the lower opening of the transparent tube cover; The plastic cup is a transparent structure with a closed bottom and an open top with a caliber larger than the maximum diameter of the bound transparent tube cover; the plastic cup contains water or rice nutrition that accounts for one-third of its volume. liquid; The bottom box is an airtight and light-proof structure, and its height is less than the height of the plastic cup and greater than half of the height of the plastic cup; the top surface of the bottom box is evenly opened with placement holes for placing the plastic cup; The diameter of the placement hole is larger than the bottom diameter of the plastic cup and smaller than the top diameter of the plastic cup; the bundled transparent tube cover is placed in the plastic cup and placed in the plastic cup together with the plastic cup. Place in the hole. 2. The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics as claimed in claim 1, characterized in that: the single transparent tube cover is made of acrylic plastic or glass or quartz tube, and its length is 15-30 cm. The caliber is 2-4cm. 3. The device suitable for isolated rearing of rice planthoppers and observation of biological characteristics as claimed in claim 1, characterized in that: the mesh density of the air-permeable gauze is 80-100 mesh; the cotton fills the transparent The gap between the lower opening of the tube cover and the upper 2-4cm. 4. The device suitable for isolated rearing of rice planthoppers and observation of biological characteristics as claimed in claim 1, characterized in that: the height of the plastic cup is 15 cm, the diameter of the bottom is 5-6 cm, and the diameter of the top is 8-10 cm. 5. The device suitable for isolated rearing of rice planthoppers and observation of biological characteristics according to claim 1 or 4, characterized in that: the material of the plastic cup is PET or PP transparent plastic. 6. The device suitable for isolated breeding of rice planthoppers and observation of biological characteristics according to claim 1, characterized in that: the height of the bottom box is 8-14 cm; the diameter of the placement hole is 6-8 cm. 7. The device suitable for isolated rearing of rice planthoppers and observation of biological characteristics as claimed in claim 1, characterized in that: said transparent tube cover is composed of more than one transparent tube cover monomers bound by rubber bands; each The air-permeable gauze at the opening on the transparent tube cover is wound and fixed by rubber bands.