CN107549121A - The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus - Google Patents
The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus Download PDFInfo
- Publication number
- CN107549121A CN107549121A CN201710895339.6A CN201710895339A CN107549121A CN 107549121 A CN107549121 A CN 107549121A CN 201710895339 A CN201710895339 A CN 201710895339A CN 107549121 A CN107549121 A CN 107549121A
- Authority
- CN
- China
- Prior art keywords
- bottle
- water
- raising
- planthopper
- rice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of breeding apparatus of indoor feeding planthopper and its application, breeding apparatus to include：Raise bottle, water accumulating disc and be placed on and be used for the bottle holder for supporting raising bottle in water accumulating disc；The raising bottle is used to cultivate rice and raising planthopper, including：Transparent vial body, bottom have bottleneck, and top has transom；Bottom of bottle lid, it is detachably connected with the bottleneck, for support rice seedling raising ground substance soil, bottom is provided with some through holes；The bottle holder has at least one groove for being used to place raising bottle, the bottom portion of groove perforate；Water accumulating disc, for holding the water needed for paddy growth；In use, the bottom of bottle lid contacts with the water in water accumulating disc.The invention also discloses the method using above-mentioned breeding apparatus raising planthopper.The breeding apparatus of the present invention is reasonable in design, make it have volume is light and handy, save space and it is easy to operate the advantages that, be advantageous to the large-scale breeding of planthopper.
Description
Technical field
The present invention relates to technical field of insect feeding, more particularly to a kind of method for breeding of indoor feeding planthopper, raising
The application of device and breeding apparatus.
Background technology
Planthopper is one of important pests for endangering Asia rice, can be survived in China South And North Rice Regions.Planthopper includes
Following species：Brown paddy plant hopper (Nilaparvata lugens), white backed planthopper (sogatella furcifera) and small brown rice planthopper
(Laodelphax striatellus).All kinds of planthoppers, which can directly pierce, to be inhaled rice strain juice or is propagated in the form of insect vector more
Kind Virus Diseases of Rice damages.Three kinds of field, plant hopper often alternately cause disaster by generation outburst, and turning into influences China's Rice Production
Mostly important limiting factor.Therefore, ecology and physiological mechanisms, the generation for effectively controlling planthopper of Planthopper Outbreaks are studied
The task of top priority of rice safety production is turned into harm.
Items research is required to indoors artificial raising planthopper, can be divided into short-term raising and the class of Long-term breeding two.It is short-term to raise
Such as insecticide biologicall test is supported, the test worm after chemicals treatment need to be raised, observes 1-5d survival or behaviouristics performance.Long-term breeding
If biological experiment is in order to determine near-isogenic rice lines, test worm life-span or egg laying amount, hatching amount, need to planthopper into (if) worm colony
Raising, single head raising, female adult are laid eggs raises rice seedling to nymph hatching etc. after rice seedling, and it is big that more situations are that research needs to prepare
Different larval instar and age consistent nymph or adult are measured, generally requires continuous raising 6-20d or longer time.
Each laboratory is limited by respective condition, and the method for breeding taken is also inconsistent, is had using aluminium alloy or wooden material
The large-scale insect cage that matter makes, plant hopper is raised with reference to Turnover Box, though a large amount of test worms can be obtained, and maintains feeding time longer,
Taken up too much space, also needed between specially foster worm, acquisition test worm age is easily overlapping, and different lines easily mix, and feeding process is controllable
Property it is poor, rice seedling and test worm density are easily unbalance, obtain worm amount it is easily out of control.Have and directly raise planthopper using man-made feeds, raise
Often survival rate is low during supporting.
The method of more use is to cultivate rice strain raising planthopper indoors, and rice strain is remained by rice nutrition liquid or root
Grain endosperm is supported, also useful to contain the foster worm of the direct rice cultivation of rice soil flowerpot, institute with the material moisturizing such as agar, cotton balls, sponge
There are light-passing plastic cover, teat glass, glass beaker etc. using sealing planthopper device.But it is higher using nutrient solution cost, prepare
Complexity, and the effective root of hair of rice seedling can not be promoted to ensure rice normal growth, the long potential difference of rice seedling；Though it can be changed using soil raising rice seedling
Be good at gesture, but soil seedling need it is larger with a large amount of soil flowerpots, space-consuming；Glassware is frangible, the easily remaining mist pearl of tube wall,
Humidity is high, and rice seedling is easily mouldy, influences test worm growth and result observation.
Publication No. CN103782968B Chinese patent literature discloses a kind of rice insect rearging cage, the rearging cage bag
Cage body and seedlings nursing plate are included, cage body is the box structure being made by polylith acrylic board, and cage body front is symmetrical above and below to be provided with
Two movable cage doors, cage door are plate, and cage body front is provided with the slot coordinated with corresponding plate transverse splicing, the cage body back side
It is provided with fly net, the bottom and top in cage body are symmetrically arranged with two neck knots for being respectively used to lay corresponding seedlings nursing plate
Structure, the iron wire frame for fixing blotting paper is provided with seedlings nursing plate.The rearging cage uses nutrient solution to provide nutrition, rice seedling for rice seedling
Long potential difference；And need to open raising cage door when watering, insect that is cumbersome and raising easily escapes or mixed.
In order to improve indoor feeding planthopper effect, it is badly in need of a kind of reasonable in design, the light and handy breeding apparatus of volume and one kind
Method for breeding that is easy to operate, meeting experiment needs.
The content of the invention
The invention provides a kind of breeding apparatus of indoor feeding planthopper, the breeding apparatus is reasonable in design, makes it have
Volume is light and handy, save space and it is easy to operate the advantages that, be advantageous to the large-scale breeding of planthopper.
The invention provides following technical scheme：
A kind of breeding apparatus of indoor feeding planthopper, including：Raise bottle, water accumulating disc and be placed in water accumulating disc and be used for
The bottle holder of support raising bottle；
The raising bottle is used to cultivate rice and raising planthopper, including：
Transparent vial body, bottom have bottleneck, and top has transom；
Bottom of bottle lid, it is detachably connected with the bottleneck, for support rice seedling raising ground substance soil, bottom is provided with some through holes；
The bottle holder has at least one groove for being used to place raising bottle, the bottom portion of groove perforate；
Water accumulating disc, for holding the water needed for paddy growth；
In use, the bottom of bottle lid contacts with the water in water accumulating disc.
The breeding apparatus of the present invention is reasonable in design.It is used to stack rice seedling raising ground substance soil in bottom of bottle lid, Rice Cropping is being educated
In seedling substrate soil, root system can pass through the through hole of bottom of bottle lid and bottle holder groove to be grown into water accumulating disc, and rice shoot is in transparent vial body
Growth, while be used to raise planthopper in transparent vial body；The bottom of raising bottle is placed in the groove of bottle holder, and raising bottle passes through bottle
Bracket is located above water accumulating disc, while bottom of bottle lid contacts with the aqueous phase in water accumulating disc, and the substrate soil in bottom of bottle lid is protected by absorbing
Hold the moisture needed for paddy growth.Only need timing to add water into water accumulating disc during planthopper is raised, be not required to open
Raise bottle；Use rice seedling raising ground substance soil to carry overnutrition, well developed root system, and rice root for rice simultaneously and may pass through bottom of bottle lid
Grown with the through hole of bottle holder bottom portion of groove into water accumulating disc, rice seedling keeps good growing way for a long time；Different larval instar planthopper is growing
Period isolates with rice moisture, can reduce humidity, improves survival rate.
Preferably, the material of the transparent vial body is plastics.
Plastic density is smaller, transparency is high so that breeding apparatus is lighter.
The transparent vial body can be cylinder or square column type, it is preferred that the volume of the transparent vial body be 200~
500ml, it is highly 10~15cm.Here the distance at the top of bottleneck to bottle is highly referred to.
The bottle of the size can meet the growth of rice, and and can reduces it and taken up space as far as possible.
Preferably, described transom is the opening at the top of transparent vial body, and opening cover sets the nylon wire of 60~100 mesh
Cloth.
It is further preferred that using rubber band by nylon net cloth lock ring in the opening of transparent vial body, transparent vial body opening
The side wall outwardly convex at place, prevents rubber band from slipping.
The setting of the structure be easy to rice seedlings and planthopper into and out.
Preferably, the internal diameter of the bottom of bottle lid is 2~3cm.
Rice seedling planting amount is limited when the internal diameter of bottom of bottle lid is too small；When internal diameter is excessive, when raising bottle is toppled over or is inverted, bottle
Easily abjection takes rice seedling out of to seedling raising ground substance soil in bottom simultaneously.
Preferably, the through-hole diameter that bottom of bottle covers is 0.2~0.4cm.
Preferably, the bottom of bottle lid is connected with whorl of bottleneck.It is easy to disassemble using being threadedly coupled.
Preferably, the depth of groove on the bottle holder is 3~5cm；The bottom complementary configuration of groove and raising bottle.
The technical scheme ensure that the stability that raising bottle is placed on bottle holder.
Preferably, the quantity of the groove on the bottle holder is 1~64, it is further preferred that the quantity of groove is 4~16.
Preferably, the depth of the water accumulating disc is 3~5cm.
The breeding apparatus of the present invention can be used for unified expanding propagation raising to obtain the planthopper of identical age, can be also used for
Planthopper is grown for Resistance detecting experiment, insecticide on planthopper and offspring's reproductive capacity influences experiment etc..
The invention also discloses the method using above-mentioned breeding apparatus raising planthopper, including：
(1) rice seedling raising ground substance soil is mixed into pasty state according to 1: 1~3 mass ratio with water, takes 2~5g to be placed on bottle
In bottom；
(2) rice paddy seed is seeded on rice seedling raising ground substance soil, the application rate of each bottom of bottle lid is 1~15, will be broadcast
Bottom of bottle lid after kind is positioned in the water accumulating disc being filled with water, and is put in growth cabinet and is cultivated；
Preferably, condition of culture is：26~28 DEG C of temperature, light dark time are 16h/8h, humidity 60~80%.
Preferably, seed soaking and vernalization are also included before sowing：Rice paddy seed is soaked seed 36~48h at 25~27 DEG C, afterwards
Drain away the water, 25~30 DEG C of vernalization show money or valuables one carries unintentionally or sprouted to rice paddy seed long to 0.2~0.5cm.
The rice paddy seed of suitable kind can be selected according to specific needs.As brown paddy plant hopper selects elegant water 11 or TN1, white backed planthopper
From TN1, small brown rice planthopper selects Wu-Yu-Geng 3.
(3) as 5~10cm of Rice Seedling Height, bottom of bottle lid is screwed in into transparent vial body；The planthopper for needing to raise is accessed and raised
Support in bottle；
(4) the raising bottle for accessing rice seedlings and planthopper is positioned in the groove of bottle holder, bottle holder is placed on water accumulating disc
In, suitable quantity of water is added in water accumulating disc makes bottom of bottle lid be contacted with water, is integrally put into growth cabinet and raises.
Preferably, added water into water accumulating disc every 2~3 days, bottom of bottle lid is contacted with water.
Preferably, rearing conditions are：26~28 DEG C of temperature, light dark time are 16h/8h, humidity 60~80%.
In feeding process, when raise rice seedling chlorosis in bottle or it is withered when, the rice seedling that need to more renew, realize continuous raise
Support.
Change seedling-growing method：Topple over or be inverted raising bottle, flick bottom of bottle lid, the planthopper in raising bottle is dropped into transparent vial body
Bottle wall or bottle top, screw out bottom of bottle lid and will fill the bottom of bottle lid of new rice seedling again and screw in transparent vial body, form new rice seedling bottle.
Compared with prior art, beneficial effects of the present invention are：
(1) breeding apparatus of the invention is reasonable in design, and rice seedling raising ground substance soil is contained in bottom of bottle lid, and substrate soil provides for rice
Nutrition needed for growth so that rice root of hair is fast, rice shoot is sturdy, and growing way is better than using artificial medium and common rice earth culture
The growing way of foster rice；
(2) rice root may pass through bottom of bottle lid and the through hole of bottle holder bottom portion of groove grows into water accumulating disc, well developed root system, rice
Seedling growing way is preferable；
(3) only need timing disposably to add water into water accumulating disc during planthopper is raised, be not required to open raising
Bottle, saves the time, while avoids the planthopper escape of raising or mix.
Brief description of the drawings
Fig. 1 is the cross-sectional view of the breeding apparatus of indoor feeding planthopper.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.
The breeding apparatus of indoor feeding planthopper includes raising bottle, bottle holder 2 and water accumulating disc 1.
Water accumulating disc 1 is the vinyl disc of (25~30) cm × (25~30) cm specifications, is highly 5cm, bottle holder 2 is placed on ponding
In disk.
Bottle holder is positioned in water accumulating disc, and bottle holder 2 is 30cm × 30cm plastic plate, has several grooves 3 on bottle holder 2,
It is preferred that 4 × 4, the bottom of groove 3 has a diameter of 0.5cm perforate.
Raising bottle includes transparent vial body 4 and bottom of bottle lid 5, and the bottleneck of transparent vial body 4 is threadedly coupled with bottom of bottle lid 5, bottom of bottle lid 5
Bottom be provided with through hole.The bottom profile and the complementation of groove 3, raising bottle for raising bottle can be placed into groove 3.
Transparent vial body 4 is plastic material, and volume is 200~500ml, is highly 10~15cm.
Bottleneck diameter is 2cm, and the internal diameter of bottom of bottle lid 5 is slightly larger than 2cm, and bottom has 0.2~0.4cm perforate.Bottom of bottle lid 5
Bottom is used to hold as rice seedling raising ground substance soil.
The open top of bottle is raised, open-mouth is covered with the nylon net cloth 6 of 60~100 mesh, and nylon is fixed using rubber band
Screen cloth 6.
In use, bottle holder 2 is placed in water accumulating disc 1, raising bottle is positioned in the groove 3 of bottle holder 2.Filled in water accumulating disc 1
Water, the bottom of bottom of bottle lid 5 contact with water.
Use the method for above-mentioned breeding apparatus raising planthopper for：
Step 1：Rice seedling bottle prepares
1) rice seed soaking and vernalization：
According to raised planthopper species, rice varieties to be broadcast are respectively from corresponding sense worm kind, as brown paddy plant hopper is selected
Elegant water 11 or TN1, white backed planthopper select TN1, and small brown rice planthopper selects Wu-Yu-Geng 3.It is preferred that the full healthy seed rice of appearance, clear water rinsing
Shrivelled grain impurity is removed, 25-27 DEG C of seed soaking about 48h, leaches excessive moisture afterwards, wet towel covers, 30 DEG C of vernalization about 48h,
It is long i.e. available to 0.2-0.5cm to treat that most of paddy shows money or valuables one carries unintentionally or sprouted.
2) sow
By rice substrate soil：The mass ratio that water is 1: 2, which infiltrates matrix, to be mixed, and medication bale-out about 2-5g pasty state matrix is put
In bottom of bottle base portion, base is not paved with, convex is formed, sprouting rice point is sowed at convex region, the application rate per bottom
Need to be according to later experiments needs, optional 10 germinations grain.After planting bottom, which is arranged in be filled with water to cultivate in water accumulating disc, (stays water layer
1cm)；It is positioned in growth cabinet, setting rearing conditions are 27 DEG C ± 1 of temperature, and the light dark time is 16h/8h, RH is 70 ±
10%.Rearing conditions are identical with this after generally connecing test worm.
3) combination of bottle is raised：Rice sprout has that 5-10cm is high after sowing 4-5d, and hand rest acts the bottom of bottle lid for containing rice seedling, makes rice seedling depth
Enter in transparent vial body, bottom of bottle lid screw thread merges composition raising bottle with transparent vial body mouth cylindrical part screw thread, is placed on 16 hole bottle holder, applies
The nylon gauze of (10-15) cm × (10-15) cm 60~100 mesh, bottle holder are placed in water accumulating disc and (stay water layer 1-3cm).Use
When water accumulating disc add water to be highly not less than bottle cap bottom surface, not higher than bottle cap top surface so that rice substrate soil does not have water and oozed out.Manually
Typically add within 2-3 days water 300-500mL in climate box, avoid adding water to waste time and energy by basin during single basin seedling raising.
In the case where growth cabinet is raised, rice seedling root of hair stretches out from bottom hole to absorb water the rice seedling bottle made, can
Good growing way is kept in 30d.Bottle intracavitary bottom substrates are constantly in pasty state and are close to bottom of bottle, and rice base root system is preferably adhered to bottom bottom
Portion, part root system also bunchy drag through hole to stretch into water accumulating disc from bottom and bottle, and bottle stromal surface is without ponding, rice seedling root system, water
Rice substrate soil and bottom are integrally formed so that are fallen if bottle rice seedling is met, are inverted and flap, will not be slipped from bottom.
Step 2：Connect worm
Method 1, before band bottom seedling merges with bottle, first by 10 × 10cm screen cloths on bottle overhead guard, rubber band binds mouth,
It by first emergence female adult 1-3 heads and 1-2 head male worm inhalers, then will merge with seedling bottom of bottle lid with bottle, be placed on bottle holder.
Method 2, ready rice seedling bottle is kept flat into a row on ceramic whiteware disk, with desk lamp irradiation at bottom of bottle, open bottle top
Rubber band and screen cloth, test worm is suctioned out or sucked.
Step 3：Change seedling
When more because of worm amount during nymph is raised, test worm is changed with rice seedling mode is changed.Change seedling-growing method：Topple over or be inverted rice
Seedling bottle, with desk lamp irradiation at bottle top, bottom of bottle lid is flicked, planthopper is dropped into bottle wall or bottle top, screw out old bottom seedling to be changed,
Hand rest plays the preprepared fresh rice seedling bottom of Sheng, rice seedling is goed deep into bottle, bottom of bottle lid screw thread and transparent vial body bottle mouth position
Screw thread merges, and forms rice seedling bottle.Worm is supported by changing seedling, avoids artificial fluke from causing test worm to lose or mix.
Application examples 1 is continuous a large amount of to be obtained brown paddy plant hoppers and unifies age into, nymph
1st, shrivelled grain impurity, 25-27 are removed from the full health of appearance, the TN1 seed rice of brown paddy plant hopper sense worm, clear water rinsing
DEG C seed soaking about 48h, leaches excessive moisture, wet towel covering, 30 DEG C of vernalization about 48h, treats most of paddy budding about 0.2- afterwards
0.5cm is i.e. available；
By rice matrix：The mass ratio that water is 1: 2, which infiltrates matrix, to be mixed, and about 10 grams of pasty state matrix of medication bale-out are placed in
Bottom of bottle base portion, without being paved with base, convex is formed, sprouting rice point is sowed at convex region, after planting by bottom of bottle lid
The water accumulating disc that is filled with water is arranged in, is positioned in growth cabinet and cultivates.12-15 germination grain of application rate per bottom of bottle lid；
Bottom of bottle lid is screwed in when rice sprout has 5cm high left and right after 4-5d and gone in the mineral water bottle of bottom, rice seedling bottle is formed, is placed on
On 16 hole bottle holder, (10-15) cm × (10-15) cm nylon gauzes are applied, even bottle holder is placed in water accumulating disc and (stays water layer 1-2cm),
Every 3d waterings once；
2nd, screen cloth on bottle overhead guard, rubber band sealing.In 3 female adults that first will just sprout wings and 1-2 head male worm inhalers, test worm is treated
Follow-up breeding female is re-used as after mating 3d, worm is connect with 2-3 heads breeding female/bottle, is placed on bottle holder, 2d changes seedling once.
Fluke method can also be taken：Bottle is kept flat into a row on ceramic whiteware disk, with desk lamp irradiation at bottom of bottle, opens bottle top
Rubber band and screen cloth, test worm is suctioned out or sucked.
3rd, when more because of worm amount during test worm is raised, replace shifting test worm with rice seedling mode is changed, change seedling-growing method：Topple over or
Rice seedling bottle is inverted, with desk lamp irradiation at bottle top, bottom is flicked, planthopper is dropped into bottle wall or bottle top, then more renew
Bottom seedling.
The condition of culture of growth cabinet：Temperature is 27 ± 1 DEG C, and light dark 16h/8h, RH are 70 ± 10%, continuous training
Educate.
Conclusion：Implemented by this programme, to broadcast 10 plants of rice/bottles, access breeding female 2-3 heads/bottle, totally 16 bottles/disk, produced
Female adult is removed after ovum 2d, the brown paddy plant hopper obtained unifies scheduled time age and quantity is shown in Table 1.
Table 1 obtains brown paddy plant hopper and unifies scheduled time age and quantity
The Resistance detecting of the different rice germplasm Whitebacked Planthoppers of application examples 2
Worm is connect by 3 bosom ovum white backed planthopper female adult/bottles, 2d changes seedling or moves worm once, and spawning seedling continues raising 10-12d and obtained
Obtain 50-80 heads/bottle 2-3 age WBPH test worms；
At the same time, different rice varieties (white backed planthopper sense worm TN1, the pest-resistant seed rice of spring river 06 and rice seed to be detected are taken
Matter) equal more than 40 grain, it is put in kraft paper bag, 25-27 DEG C of seed soaking about 48h, leaches excessive moisture afterwards, wet towel covering, 30 DEG C
Vernalization about 48h, treat that most of paddy budding about 0.2-0.5cm is i.e. available；
By rice matrix：The mass ratio that water is 1: 2, which infiltrates matrix, to be mixed, and about 3-5 grams pasty state matrix of medication bale-out is put
In bottom of bottle base portion, without being paved with base, convex is formed, sprouting rice point is sowed at convex region, 4 are seeded in per kind
In individual bottom of bottle lid, it is 10 germination grain per the application rate of bottom of bottle lid, is positioned in culture dish, culture dish numbering, after planting contains
Bottom culture dish adds water to be positioned over growth cabinet raising；
Bottom is screwed in and gone in the mineral water bottle of bottom by rice seedling when rice sprout has 6-10cm high left and right after 4-5d, forms rice seedling bottle
Body, it is placed on 16 hole bottle holder, applies 40 × 40cm nylon gauzes, even bottle holder is placed in water accumulating disc and (stays water layer 1-2cm), every 3d
Watering is once；
Every bottle of actual strain number of emergence is checked, by 10 2-3 ages nymph/strains, calculating connects worm amount, the test worm that will have been bred in advance
Clap in ceramic whiteware disk, count and test worm access is loaded with the bottle of seedling to be detected, raise about 5-8d.Per kind total strain number 20-40 strains.
Typically in 90% TN1 death, start the fruit that comes to an end.
Using this method, it is possible to reduce when rice germplasm seedling resistance is identified, different germplasm rice strain bud ratios are different, connect worm
The amount error whard to control brought.Obtaining rice seedling has good growing way, can be very well according to the anti-sense quality dead seedling number for meeting 7d after worm
Reflect rice seedling resistance level (being shown in Table 2) to be detected.
The resistance of the different rice germplasm Whitebacked Planthoppers of table 2
The insecticide of application examples 3 incubates worm amount to brown paddy plant hopper F2 generations to be influenceed
1st, research object
Laboratory rearing does not contact the brown paddy plant hopper strain of any medicament for many years.
2nd, method
Test worm is handled using dipping seedling method and carries out female adult reproductive capacity measure, it is specific as follows：
It is prepared by 2.1 rice seedling bottles：From brown paddy plant hopper sense worm TN1 seed rice, 25-27 DEG C of seed soaking about 48h, superfluous water is leached afterwards
Point, wet towel covering, 30 DEG C of vernalization about 48h, treat that most of paddy budding about 0.2-0.5cm is i.e. available.
By rice matrix：The mass ratio that water is 1: 2, which infiltrates matrix, to be mixed, and about 3-5 grams pasty state matrix of medication bale-out is put
In bottom of bottle base portion, without being paved with base, convex is formed, sprouting rice point is sowed at convex region, after planting by bottom of bottle
Lid is arranged in the water accumulating disc that is filled with water, and is positioned over artificial gas raising.
10 germination grain of application rate per bottom.Rice seedling screws in bottom when rice sprout has 6-10cm high left and right after 4-5d
Go in the mineral water bottle of bottom, form rice seedling bottle, be placed on 16 hole bottle holder, apply 40 × 40cm nylon gauzes, even bottle holder disk is placed in
(water layer 1-2cm is stayed in water accumulating disc), every 3d waterings once.
2.2 test worms expand numerous.3 breeding female/bottles are connect into worm, will go deep into bottom seedling from bottom of bottle mouth, tighten bottom afterwards,
It is placed on bottle holder, 2d changes seedling, and spawning seedling, which is put into new bottle, to be continued to raise, if 18-20d obtains 30-50 heads/bottle 5 age brown paddy plant hopper
Worm.In 2-4 periods in age, bottom seedling need to be changed 1-2 times.
2.3 medicaments and its dilution.Reagent agent is 95% pymetrozine active compound (PYM), and 5-6 are formulated as by solvent of DMF
Pymetrozine gradient dilution liquid.
The processing of 2.4 test worms：More than 10 strain rice shoots are transplanted in advance in the small basins of diameter 11cm, treat its length to 30cm height, trimming
Stay band cauline leaf seedling 20cm high, it is overnight standby to outwell water layer.Rice seedling is inverted to 20s in the 250mL graduated cylinders of deep Sheng medicine, horizontal successively
Naturally dry, bilateral printing opacity barrel cover is put, often processing sets 3-4 and repeated.5 latter stage in age nymphs 30 after access is selected per basin, 3d
Afterwards, test worm is poured on ceramic whiteware disk by basin, sorts female, male, 5 age nymphs, original rice seedling basin is put back to by the pairing of 5: 3 female-male proportions
In, remaining test worm is removed, and generally effectively female borer population is about 10-15 heads, then is raised 3 days, emergence female adult is completed mating.
2.5 identification female adult F2 incubate worm amount
It will be respectively connected in the standby rice seedling bottle of institute, 1 every bottle, be sealed with 80 mesh gauzes, per diary per basin post-coitum female adult
Survival condition is recorded, death in 1d is considered as invalid female adult, needs to reject.Female adult is removed after spawning 6d, seedling continues to raise after spawning, often
Worm appearance is incubated every 2-3d observations whether there is, 9-10d observation hatching numbers, every bottle of test worm is beaten in into ceramic whiteware disk, artificial range estimation counting hatching
If borer population.
3 result inspections
Determine PYM to brown paddy plant hopper F2 for nymph hatching amount when need raise spawning seedling, must connect to inspection result from worm is connect
Continuous raising rice seedling 15d, rice seedling growing way must keep healthy and strong during raising, otherwise can interference test result.Using this method, obtain
Rice seedling has good growing way, and acquired results can reflect influences (be shown in Table 3) of the various dose PYM to brown paddy plant hopper F2 nymph amounts very well.
The influence of worm amount is incubated in the various dose PYM of table 3 processing on brown paddy plant hopper F2
Claims (10)
- A kind of 1. breeding apparatus of indoor feeding planthopper, it is characterised in that including：Raise bottle, water accumulating disc and be placed on product It is used for the bottle holder for supporting raising bottle in water pond；The raising bottle is used to cultivate rice and raising planthopper, including：Transparent vial body, bottom have bottleneck, and top has transom；Bottom of bottle lid, it is detachably connected with the bottleneck, for support rice seedling raising ground substance soil, bottom is provided with some through holes；The bottle holder has at least one groove for being used to place raising bottle, the bottom portion of groove perforate；Water accumulating disc, for holding the water needed for paddy growth；In use, the bottom of bottle lid contacts with the water in water accumulating disc.
- 2. breeding apparatus according to claim 1, it is characterised in that the volume of the transparent vial body is 200~500ml, Highly it is 10~15cm.
- 3. breeding apparatus according to claim 1, it is characterised in that described transom is opening at the top of transparent vial body Mouthful, opening cover sets the nylon net cloth of 60~100 mesh.
- 4. according to the breeding apparatus described in any one of claim 1, it is characterised in that the internal diameter of the bottom of bottle lid is 2~3cm.
- 5. breeding apparatus according to claim 4, it is characterised in that the depth of groove on the bottle holder is 3~5cm；It is recessed The bottom complementary configuration of groove and raising bottle.
- 6. a kind of breeding apparatus according to any one of Claims 1 to 5 obtains identical age in unified expanding propagation raising Planthopper, rice the experiment of the Resistance detecting of planthopper or insecticide are grown on planthopper and offspring's reproductive capacity influence it is real Application in testing.
- 7. a kind of method that breeding apparatus using described in any one of Claims 1 to 55 raises planthopper, it is characterised in that bag Include：(1) rice seedling raising ground substance soil is mixed into pasty state according to 1: 1~3 mass ratio with water, takes 2~5g to be placed on bottom of bottle lid It is interior；(2) rice paddy seed is seeded on rice seedling raising ground substance soil, the application rate of each bottom of bottle lid is 1~15, will after planting Bottom of bottle lid be positioned in the water accumulating disc being filled with water, be put in growth cabinet and cultivate；(3) as 5~10cm of Rice Seedling Height, bottom of bottle lid is screwed in into transparent vial body；Bottle is raised into the planthopper access for needing to raise In；(4) the raising bottle for accessing rice seedlings and planthopper is positioned in the groove of bottle holder, bottle holder is placed in water accumulating disc, product Suitable quantity of water is added in water pond makes bottom of bottle lid be contacted with water, is integrally put into growth cabinet and raises.
- 8. the method for raising planthopper according to claim 7, it is characterised in that also include seed soaking and vernalization before sowing： Rice paddy seed is soaked seed 36~48h at 25~27 DEG C, drained away the water afterwards, 25~30 DEG C of vernalization show money or valuables one carries unintentionally or gone out to rice paddy seed Bud length is to 0.2~0.5cm.
- 9. the method for raising planthopper according to claim 7, it is characterised in that in step (4), every 2~3 days vector products Add water in water pond, bottom of bottle lid is contacted with water.
- 10. the method for raising planthopper according to claim 7, it is characterised in that in step (2) neutralization procedure (4), people Environmental condition in work climate box is：26~28 DEG C of temperature, light dark time are 16h/8h, humidity 60~80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710895339.6A CN107549121A (en) | 2017-09-27 | 2017-09-27 | The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710895339.6A CN107549121A (en) | 2017-09-27 | 2017-09-27 | The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107549121A true CN107549121A (en) | 2018-01-09 |
Family
ID=60983207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710895339.6A Pending CN107549121A (en) | 2017-09-27 | 2017-09-27 | The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107549121A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371149A (en) * | 2018-04-27 | 2018-08-07 | 福建省农业科学院植物保护研究所 | A kind of device and its application method of interior mass rearing planthopper |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1020945A (en) * | 1964-04-30 | 1966-02-23 | Lustroid Ltd | Improvements relating to plant pots |
| JP2006254826A (en) * | 2005-03-18 | 2006-09-28 | Yasuoka Shizen Kankyo Hozen Kenkyusho:Kk | Rearing kit for firefly |
| CN201928700U (en) * | 2010-11-22 | 2011-08-17 | 曾惠兴 | Soil self-moistening type plant cultivation pot |
| CN201967454U (en) * | 2011-01-04 | 2011-09-14 | 陈强 | Culture vessel for water cultivation of plant |
| CN102669061A (en) * | 2012-06-04 | 2012-09-19 | 云南省热带作物科学研究所 | Parasitoid wasp adult rearing cage |
| CN203952165U (en) * | 2014-07-21 | 2014-11-26 | 傅淑 | A kind of new device of raising piercing sucking insect |
| CN204335590U (en) * | 2014-11-24 | 2015-05-20 | 山西省农业科学院植物保护研究所 | The chrysomelid larva raising observation device of grain |
| CN103782968B (en) * | 2014-02-26 | 2015-06-10 | 福建农林大学 | Acrylic rice insect breeding cage and rice insect breeding method |
| CN204949265U (en) * | 2015-09-25 | 2016-01-13 | 贵州省植物保护研究所 | Detect device of planthopper to medicament sensitiveness |
| CN105794724A (en) * | 2016-03-14 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Method for conveniently observing and feeding rice planthoppers |
| CN206227400U (en) * | 2016-11-30 | 2017-06-09 | 四川农业大学 | A kind of device raised suitable for planthopper and observed |
| CN207355280U (en) * | 2017-09-27 | 2018-05-15 | 中国水稻研究所 | A kind of breeding apparatus of indoor feeding planthopper |
-
2017
- 2017-09-27 CN CN201710895339.6A patent/CN107549121A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1020945A (en) * | 1964-04-30 | 1966-02-23 | Lustroid Ltd | Improvements relating to plant pots |
| JP2006254826A (en) * | 2005-03-18 | 2006-09-28 | Yasuoka Shizen Kankyo Hozen Kenkyusho:Kk | Rearing kit for firefly |
| CN201928700U (en) * | 2010-11-22 | 2011-08-17 | 曾惠兴 | Soil self-moistening type plant cultivation pot |
| CN201967454U (en) * | 2011-01-04 | 2011-09-14 | 陈强 | Culture vessel for water cultivation of plant |
| CN102669061A (en) * | 2012-06-04 | 2012-09-19 | 云南省热带作物科学研究所 | Parasitoid wasp adult rearing cage |
| CN103782968B (en) * | 2014-02-26 | 2015-06-10 | 福建农林大学 | Acrylic rice insect breeding cage and rice insect breeding method |
| CN203952165U (en) * | 2014-07-21 | 2014-11-26 | 傅淑 | A kind of new device of raising piercing sucking insect |
| CN204335590U (en) * | 2014-11-24 | 2015-05-20 | 山西省农业科学院植物保护研究所 | The chrysomelid larva raising observation device of grain |
| CN204949265U (en) * | 2015-09-25 | 2016-01-13 | 贵州省植物保护研究所 | Detect device of planthopper to medicament sensitiveness |
| CN105794724A (en) * | 2016-03-14 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Method for conveniently observing and feeding rice planthoppers |
| CN206227400U (en) * | 2016-11-30 | 2017-06-09 | 四川农业大学 | A kind of device raised suitable for planthopper and observed |
| CN207355280U (en) * | 2017-09-27 | 2018-05-15 | 中国水稻研究所 | A kind of breeding apparatus of indoor feeding planthopper |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371149A (en) * | 2018-04-27 | 2018-08-07 | 福建省农业科学院植物保护研究所 | A kind of device and its application method of interior mass rearing planthopper |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102318547B (en) | Overhead cultivation method of Dendrobium huoshanense test-tube seedlings | |
| Barker et al. | Growing M. truncatula: choice of substrates and growth conditions | |
| CN207355280U (en) | A kind of breeding apparatus of indoor feeding planthopper | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN101796939A (en) | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof | |
| CN105993865A (en) | Cultivation method for quercus variabilis aseptic seedling | |
| Cho et al. | Seed dormancy and germination characteristics of the endangered species Cicuta virosa L. in South Korea | |
| CN107549121A (en) | The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus | |
| CN108902052A (en) | A kind of method of cotten aphid indoor bioassay | |
| CN103918611B (en) | A kind of easy wheat aphid bioassay method | |
| CN104012476A (en) | Method for massively raising laodelphax striatellus indoors | |
| CN109845705B (en) | Small parasitic wasp adult stomach toxicity and contact toxicity measuring device and using method | |
| CN108918785B (en) | Device and method for measuring toxicity of whitefly pests by using living plants | |
| CN101438693A (en) | Artificial propagation method of American periplaneta | |
| CN105145150A (en) | Plant disease resistance evaluation method, root injuring method and inoculation method | |
| CN105409887B (en) | A kind of method for breeding of palm thrips | |
| CN204949265U (en) | Detect device of planthopper to medicament sensitiveness | |
| Olson et al. | Effect of soilless potting media and water management on development of fungus gnats (Diptera: Sciaridae) and plant growth | |
| CN213486257U (en) | Salt tolerance appraisal device of sesame whole growth period | |
| CN104222024B (en) | The method of the blue or green hidden wing first of wing ant shape of artificial mass rearing | |
| CN108575912A (en) | A kind of method that evaluation insecticidal proteins influence Propylaea japonica Adults and feeding device | |
| CN110432100A (en) | Rice bitubular drought resisting identification apparatus and method based on depth layer root Stress treatment | |
| CN114145269B (en) | Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis | |
| CN106135138A (en) | A kind of device collecting Eggs of The Rice Leaf Roller and using method thereof | |
| CN104957122A (en) | Rice leaf roller acquisition and biological assay method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |