CN114831084A - Method for feeding rice planthoppers by utilizing ratoon rice - Google Patents
Method for feeding rice planthoppers by utilizing ratoon rice Download PDFInfo
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- 241000209094 Oryza Species 0.000 title claims abstract description 183
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 182
- 235000009566 rice Nutrition 0.000 title claims abstract description 182
- 241001466042 Fulgoromorpha Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000009395 breeding Methods 0.000 claims abstract description 11
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 238000003306 harvesting Methods 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 19
- 239000004202 carbamide Substances 0.000 claims description 19
- 241000238631 Hexapoda Species 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 14
- 241001498622 Cixius wagneri Species 0.000 claims description 13
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 238000009340 sequential cropping Methods 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 3
- 229940072033 potash Drugs 0.000 claims description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 3
- 235000015320 potassium carbonate Nutrition 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 241001556089 Nilaparvata lugens Species 0.000 description 8
- 241000819999 Nymphes Species 0.000 description 8
- 241000176086 Sogatella furcifera Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008929 regeneration Effects 0.000 description 4
- 238000011069 regeneration method Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a method for feeding rice planthoppers by utilizing ratoon rice, which is characterized in that after the ratoon rice is harvested, new rice seedlings can be regenerated in 7-10 days under proper conditions, and feeding and spawning places are provided for the rice planthoppers. The method for breeding the rice planthoppers by utilizing the ratoon rice has the advantages of obviously saving time, labor and expenses, and is beneficial to indoor large-scale breeding of the rice planthoppers.
Description
Technical Field
The invention belongs to the field of artificial feeding of insects, and particularly relates to a method for feeding rice planthoppers by utilizing ratoon rice.
Background
Rice is the most important food crop in China, occupies a very important position in national food production, and has the top of the world in planting area and yield. Rice planthopper (mainly brown planthopper Nilaparvatalugens)
The Sogatella furcifera (Horv th) and Laodelphax striatellus (Falen)) are one of the most main rice pests in main rice planting areas in China and south east Asia, not only seriously affect the rice yield, but also can transmit various rice virus diseases, and the rice planthoppers are a hot research object in the professional aspect of rice plant protection in view of the damage and the occurrence area.
The rice planthopper research related laboratory needs large-scale artificial feeding under the laboratory condition for the research of the biological, physiological and molecular mechanism and the like of the rice planthopper. In order to obtain rice planthoppers with more consistent biological characteristics in most laboratories, such as consistent resistance level, consistent age, consistent seed source property and other biological characteristics, long-period subculture of the rice planthoppers is required.
Rice is the main host plant for breeding rice planthopper. In order to obtain the rice planthoppers required by the experiment, a large amount of rice seedlings need to be cultivated in a laboratory. Research shows that various physiological parameters (such as the parameters of the life, the egg laying amount, the honeydew amount and the like) of the rice planthopper are superior to those of the rice in the early growth stage (such as the seedling stage and the tillering stage) in the booting stage and the later development stage of the rice. However, the rice needs to develop to the booting stage after a long time, and a better rice seedling host can be obtained only under proper indoor growth conditions (especially in winter), and the stress of the environmental factor on the growth of the rice seedling is particularly obvious in winter, thereby leading to the increase of the experimental expenditure budget. Most laboratories adopt rice seedlings in seedling stage to feed rice planthoppers, because the rice seedlings in seedling stage have weak rice planthopper invasion resistance, the hosts need to be frequently replaced, the accurate and stable feeding of the rice planthoppers is not facilitated, and the parameters of the rice planthoppers such as growth and development on the rice seedlings in seedling stage are poorer than those in later stage of the rice seedlings (such as booting stage and later growth stage). Meanwhile, in order to obtain clean host rice seedlings, the rice seedling cultivation room and the rice planthopper feeding room need independent isolation spaces. Therefore, the laboratory requires much time, space, labor and expense in order to obtain suitable rice seedling hosts.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: how to provide a method for feeding rice planthoppers indoors on a large scale, and the purposes of saving time, labor and expenses are achieved.
The technical scheme of the invention is as follows: a method for breeding rice planthoppers by utilizing ratoon rice comprises the following steps:
the method comprises the following steps: measures before harvesting of first season rice of ratoon rice: applying urea 7 days before the first season ripens, and irrigating 1-3cm after applying urea one day;
step two: harvesting first-season rice of ratoon rice: adopting a high stubble-remaining harvesting method, wherein the height of the stubble-remaining is 50-60 cm;
step three: the method comprises the following steps of (1) taking water and fertilizer management measures after harvesting of ratoon rice: covering water in the field after harvesting the ratoon rice, wherein the height of the water layer is 3-5 cm; applying additional fertilizer urea and potash fertilizer 3-7 days after harvesting the first season rice of the ratoon rice;
step four: transferring the ratooning rice indoors and performing daily management:
1) preparing: after the harvest of the regenerated rice, newly-grown regenerated rice seedlings with the height of 5-10cm are moved into the room to be fed with rice planthoppers;
2) transplanting: digging the regenerated rice stump continuous mud, transplanting the dug regenerated rice stump continuous mud into a culture container, and putting the culture container into a breeding cage with the temperature of 25 +/-2 ℃, the humidity of 75 +/-5% and the light-dark ratio of 14h to 10h in a light cycle;
3) transplanting daily management: ensuring that the height of a nutrient solution water layer in the culture container is 1-3cm in daily life;
step five: inoculating insects: inoculating the newly emerged rice planthopper imagoes into each pot of regenerated rice seedlings according to 3-5 heads of female insects and 1-3 heads of male insects; or inoculating 2-or 3-year nymphs of rice planthoppers with the same age into the breeding pond by 10-15 heads per pot;
step six: feeding:
1) if step five, adult grafting: after the next generation of newly hatched nymphs appears, the nymphs are sucked and transplanted to clean regenerated rice seedlings, and the transplanted regenerated rice seedlings can maintain the life cycle of the F0 generation adults and the incubation period of the F1 generation;
2) if the step six includes nymphs: and (4) maintaining the F0 generation nymphs to develop to the adult stage, and after the imagoes appear, inoculating the new imagoes to clean regenerated rice seedlings according to the pairing of the step five.
Further, in the first step, 5-7.5 kg of urea is applied per 667 square meters.
Furthermore, in the third step, 5-10 kg of urea and 5-7.5 kg of potassium chloride are respectively used for each 667 square meters.
Further, in the step four, 1), before transplanting, the withered leaf sheaths on the outer layers of the first-season rice stubble are stripped until the rice stems with life vitality are exposed, and meanwhile, the newly regenerated rice seedlings are checked and ensured to be free of insect pests.
Further, in step four, step 3), the nutrient solution comprises the following components: NH (NH) 4 NO 3 8.0g、KH 2 PO 4 2.5g、K 2 SO 4 3.4g、CaCl 2 3.3g and MgCl 2 1.2g, 100L of water.
The regenerated rice is the first season rice (multi-finger middle rice) which is harvested, and the lateral bud on the basal node of the rice is re-germinated, tillered and spiculated by utilizing the tillering regeneration capability of the rice stake and matching with proper fertilization, irrigation and comprehensive prevention and control of diseases, pests and weeds, so that the aim of recovering one season rice is achieved.
The variety of the regenerated rice in the invention is selected from the regenerated rice which has the characteristics of proper growth period, lodging resistance, strong regeneration capability, proper plant height, susceptibility to rice planthopper and the like.
The regenerated rice utilizes the nutrient, photosynthetic capacity and root absorption capacity of the first season rice, seedlings grow rapidly, and one seedling of the first season rice can grow 1-3 regenerated seedlings generally, so that more spaces for feeding parts, activities and natural enemies avoidance can be provided for rice planthoppers. The regenerated rice can save a large number of seeds, save water and chemical fertilizer in the aspect of water and fertilizer management, and save labor cost of a laboratory. After the first season rice of the regenerated rice is harvested, new rice seedlings can be regenerated in 7-10 days under proper conditions, and feeding and spawning places are provided for rice planthoppers. The method for breeding the rice planthopper by utilizing the ratoon rice has the advantages of obviously saving time, labor and expenses.
Compared with the prior art, the invention has the following beneficial effects:
the method for breeding the rice planthoppers by utilizing the ratoon rice has the advantages of obviously saving time, labor and expenses, and is beneficial to indoor large-scale breeding of the rice planthoppers.
Detailed Description
The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were all commercially available unless otherwise specified.
The method comprises the following steps: measures before harvesting of first season rice of ratoon rice: the urea is applied about 7 days before the first season ripens, and the water is filled for about 1-3cm after the urea is applied. And 5-7.5 kg of urea is applied to each 667 square meters about 7 days before the first season is mature to serve as a germination-protecting and accelerating fertilizer, so that the early germination of the regeneration bud can be promoted and the number of seedlings can be remarkably increased. Regenerated rice variety: the bred regenerated rice variety has the characteristics of proper growth period, lodging resistance, strong regeneration capability, proper plant height, susceptibility to rice planthopper and the like.
Step two: harvesting first-season rice of ratoon rice: adopting a high stubble-remaining harvesting method, wherein the height of the stubble-remaining is about 50-60 cm; the first season rice of the regenerated rice with small area can be harvested by adopting a manual harvesting method, and the first season rice with large area can be harvested by adopting a harvester with a narrow crawler width harvesting platform.
Step three: the method comprises the following steps of (1) taking water and fertilizer management measures after harvesting of ratoon rice: covering water in the field after harvesting the ratoon rice, wherein the height of the water layer is preferably 3-5 cm; and (3) applying additional fertilizer urea and potash fertilizer 3-7 days after harvesting the first season rice of the ratoon rice, wherein 5-10 kg of urea is applied per 667 square meters, and 5-7.5 kg of potassium chloride is applied per 667 square meters.
Step four: transplanting the ratoon rice into a laboratory and performing daily management:
1) preparing: after the regenerated rice is harvested for 10 days, newly-grown regenerated rice seedlings with the height of about 5-10cm can be transplanted into a laboratory to be fed with rice planthoppers. Before transplanting, the withered leaf sheaths on the outer layers of the rice stubble of the first-season rice are peeled off until the rice stems with life vitality are exposed. Meanwhile, whether the newly-grown regenerated rice seedlings have spawning traces of the rice planthoppers is checked, and other rice pests, natural enemies or other insects on the rice stubbles are removed.
2) Transplanting: digging a 15cm multiplied by 15cm square of the regenerated rice stump connecting mud by using a shovel, transplanting the rice stump connecting mud into a plastic basin with a proper size, and putting the rice stump connecting mud into a laboratory rearing cage with the temperature of 25 +/-2 ℃, the humidity of 75 +/-5% and the photoperiod of 14h to 10h (light: dark).
3) Transplanting daily management: the height of the water layer of the nutrient solution in the plastic basin is ensured to be about 1-3cm in daily life. The nutrient solution comprises the following components (100L of nutrient solution is required by the components and the dosage): NH (NH) 4 NO 3 (8.0g)、KH 2 PO 4 (2.5g)、K 2 SO 4 (3.4g)、CaCl 2 (3.3g) and MgCl 2 (1.2g)。
Step five: inoculating insects: inoculating the newly emerged rice planthopper adults with regenerated rice seedlings in each pot according to 3-5 female insects and 1-3 male insects; or inoculating 2-year or 3-year nymphs of rice planthoppers with the same age into the rice planthoppers with 10-15 heads/basin.
Step six: feeding:
1) inoculating adult insects: after the nymph newly hatched in the next generation appears, the nymph is sucked and transplanted to clean regenerated rice seedlings, and the inoculated regenerated rice seedlings can maintain F 0 Life cycle of adult and F 1 A second hatching period;
2) inoculating nymphs: according to the above-mentioned insect-receiving ratio the regenerated rice seedling can be maintained at F 0 And (4) the nymphs develop to the adult stage, and after the adults appear, the new imagoes are inoculated to clean regenerated rice seedlings in a pairing mode.
Application example 1: nilaparvata lugens laboratory subculture
Selecting Q Liangyou 851, and performing normal water and fertilizer and chemical control management on first-season rice; fertilizing 5.0 kg of urea/667 square meters 7d (8 months and 8 days) before harvesting, harvesting in 8 months and 15 days, and covering with water after harvesting, wherein the covering depth is 5 cm; fertilizing 7.5 kilograms of urea and 5 kilograms of potassium chloride per 667 square meters at 3d (8 months and 18 days) after harvesting, and keeping 5cm of a field shallow water layer; transferring 10d (8 months and 25 days) after harvesting into laboratory by the method, and inoculating brown planthopper (F) in two batches at 8 months and 27 days 0 Generations) adults and nymphs, respectively, were subjected to the subculture trial.
The control treatment is a common laboratory host variety TN1, and the insects are inoculated in a common laboratory seedling growth period, and each pot has about 110 rice seedlings.
Laboratory conditions during rearing: the temperature is 25 plus or minus 2 ℃, the humidity is 75 plus or minus 5 percent, and the photoperiod is 14h to 10h (light: dark).
TABLE 1 Effect of ratooning rice and TN1 rice seedlings on the subculture of brown planthopper (F) 0 Adult generation)
Note: f 1 And F 2 Data are the average of triplicate experiments; f 2 For subculture, the inoculated imagoes are F 1 Emergence of adult insects; f 1 And F 2 The nymph ratoon rice is treated and uniformly bred in 3 pots, and TN1 rice seedlings are treated and uniformly bred in 4 pots.
TABLE 2 Effect of ratooning rice and TN1 rice seedlings on the subculture of brown planthopper (nymph generation F0)
Note: f1 and F2 data are the average of three replicates; f2 is subcultured, and the grafted nymphs are nymphs of 3 rd generation F1; f 1 And F 2 The nymph ratoon rice is treated and uniformly bred in 3 pots, and TN1 rice seedlings are treated and uniformly bred in 4 pots.
And (4) conclusion: compared with the conventional method for feeding the brown planthoppers by using TN1 seedlings, the method for feeding the brown planthoppers by using the ratoon rice subculture can obtain more brown planthoppers, and has the advantages of less time consumption, low seedling changing frequency and the like.
Application example 2: laboratory subculture of sogatella furcifera
Selecting Longliangyou 248, and performing normal water and fertilizer and chemical control management on first-season rice; fertilizing 5.0 kg of urea/667 square meters 7d (8 months and 9 days) before harvesting, harvesting in 8 months and 16 days, and covering with water after harvesting, wherein the covering depth is 5 cm; fertilizing 7.5 kg/667 square meters of urea and 5 kg/667 square meters of potassium chloride 3d (8 months and 19 days) after harvesting, and keeping 5cm of field shallow water layer; transferring 10d (8 months and 26 days) after harvesting into laboratory by the method, and inoculating Sogatella furcifera (F) in two batches at 8 months and 28 days 0 Generations) adults and nymphs, respectively, and continuing the subculture test.
The control treatment is a common laboratory host variety TN1, and the insects are inoculated in a common laboratory seedling growth period, and each pot has about 110 rice seedlings.
Laboratory conditions during rearing: the temperature is 25 plus or minus 2 ℃, the humidity is 75 plus or minus 5 percent, and the photoperiod is 14h to 10h (light: dark).
TABLE 3 Effect of ratoon rice and TN1 rice seedlings on the subculture of white-backed flies (F0 generation is adults)
Note: f1 and F2 data are the average of three replicates; f2 is subcultured, and the inoculated adults are F1 newly emerged adults; f1 and F2 nymph ratoon rice treatment are bred in 3 pots, and TN1 rice seedling treatment is bred in 4 pots.
TABLE 4 influence of ratoon rice and TN1 rice seedlings on the subculture of white-backed flies (nymphs for generation F0)
Note: f1 and F2 data are the average of three replicates; f 2 For subculture, the nymph is F 1 Nymphs of generation 3; f 1 And F 2 The nymph ratoon rice is treated and uniformly bred in 3 pots, and TN1 rice seedlings are treated and uniformly bred in 4 pots.
And (4) conclusion: compared with the conventional method for feeding the sogatella furcifera by utilizing the TN1 seedling, more sogatella furcifera can be obtained by utilizing the regenerated rice subculture of the invention, and meanwhile, the method has the advantages of less time consumption, low seedling changing frequency and the like.
Therefore, compared with the conventional method for raising the rice planthoppers by using TN1 seedlings, the method for raising the rice planthoppers by using the regenerated rice subculture of the invention can obtain more rice planthoppers, and has the advantages of less time consumption, low seedling changing frequency and the like.
Claims (5)
1. A method for breeding rice planthoppers by utilizing ratoon rice is characterized by comprising the following steps:
the method comprises the following steps: measures before harvesting of first season rice of ratoon rice: applying urea 7 days before the first season ripens, and irrigating 1-3cm after applying urea one day;
step two: harvesting first-season rice of ratoon rice: adopting a high stubble-remaining harvesting method, wherein the height of the stubble-remaining is 50-60 cm;
step three: the method comprises the following steps of (1) taking water and fertilizer management measures after harvesting of ratoon rice: covering water in the field after harvesting the ratoon rice, wherein the height of the water layer is 3-5 cm; applying additional fertilizer urea and potash fertilizer 3-7 days after harvesting the first season rice of the ratoon rice;
step four: transferring the ratooning rice indoors and performing daily management:
1) preparing: after the harvest of the regenerated rice, newly-grown regenerated rice seedlings with the height of 5-10cm are moved into the room to be fed with rice planthoppers;
2) transplanting: digging the regenerated rice stump continuous mud, transplanting the dug regenerated rice stump continuous mud into a culture container, and putting the culture container into a breeding cage with the temperature of 25 +/-2 ℃, the humidity of 75 +/-5% and the light-dark ratio of 14h to 10h in a light cycle;
3) transplanting daily management: ensuring that the height of a nutrient solution water layer in the culture container is 1-3cm in daily life;
step five: inoculating insects: inoculating the newly emerged rice planthopper imagoes into each pot of regenerated rice seedlings according to 3-5 heads of female insects and 1-3 heads of male insects; or inoculating 2-year or 3-year nymphs of rice planthoppers with the same age into the nymphs at 10-15 heads per pot;
step six: feeding:
1) if step five, adult grafting: after the next generation of newly hatched nymphs appears, the nymphs are sucked and transplanted to clean regenerated rice seedlings, and the transplanted regenerated rice seedlings can maintain the life cycle of the F0 generation adults and the incubation period of the F1 generation;
2) if the step six includes nymphs: and (4) maintaining the F0 generation nymphs to develop to the adult stage, and after the imagoes appear, inoculating the new imagoes to clean regenerated rice seedlings according to the pairing of the step five.
2. The method according to claim 1, wherein in step one, 5-7.5 kg/667 m of square meter of urea is applied.
3. The method of claim 1, wherein in step three, 5-10 kg/667 m of urea and 5-7.5 kg/667 m of potassium chloride are added.
4. The method according to claim 1, wherein in step four 1), before transplanting, the withered outer layer of the first cropping rice stubble is peeled off until the alive straw is exposed, and meanwhile, the newly regenerated rice seedling is checked to ensure no insect damage.
5. The method as claimed in claim 1, wherein in step four, 3), the nutrient solution comprises the following components: NH (NH) 4 NO 3 8.0g、KH 2 PO 4 2.5g、K 2 SO 4 3.4g、CaCl 2 3.3g and MgCl 2 1.2g, 100L of water.
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