CN102696438A - Rice seedling planting method for constructing delphacidae population - Google Patents
Rice seedling planting method for constructing delphacidae population Download PDFInfo
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Abstract
The invention discloses a rice seedling planting method for constructing a delphacidae population. The rice seedling planting method comprises the following steps of: (1) performing direct seeding, i.e., flatly laying rice seeds at the bottom of a culture container, spraying water to the rice seeds to wet the rice seeds and covering the culture container to prevent the rice seeds from being dried due to evaporation of water; (2) culturing the rice seeds treated by the step (1) under the conditions of temperature of 25-30 DEG C and illumination time ratio of day to night of 14:10 hours; and (3) after rice seedlings grow to 2-3 cm, irrigating with nutrient solution and inoculating the delphacidae population. According to the rice seedling planting method, a rice planting technology is optimized for many times, the simplest and most practical planting method is screened, precious scientific and research time, space and expenditure are saved for the masses of scientific and research workers, and simple and feasible technological equipment is provided for smooth development of various principal, city and national level items. The method has the advantages of low cost, space saving, time saving, easiness in operation and capability of greatly increasing the test efficiency.
Description
Technical field
The present invention relates to the plant cultivation field, particularly relate to a kind of paddy rice seed method for planting that is used to set up the plant hopper population.
Background technology
Rice fulgorid is as the great insect on the Rice Production; Mainly comprise three kinds: brown planthopper (Nilaparvata lugens), white-backed planthopper (Sogatella furcifera) and small brown rice planthopper (Laodelphax striatellus); Their imago/nymph is except that direct thorn suction rice juice; Can also or bring out other diseases as the various virus diseases of insect-borne transmission, directly or indirectly hazard rice causes crushing loss when serious.Wherein, brown planthopper can be propagated careless shape bushy stunt and the tingia disease of stunting, and can bring out also that infecting of rice sheath blight disease, culm rot caused harm and the growing of coal smoke germ; White-backed planthopper is the popular in recent years sick vector insect of southern rice black-streaked dwarf; Small brown rice planthopper is mainly propagated stripe disease, black streaked dwarf virus of rice and MRDV poison.When retransmitting, these three kinds of plant hoppers cause serious loss for paddy rice, wheat and corn.Rice fulgorid has been classified as one of primary pest of national agriculture project, and prediction of rice fulgorid and prevention and control have become the focus of studying in China's agricultural production.The factor that influences the rice fulgorid generation because of the field is a lot, is unfavorable for the accuracy of investigation result, and many scholars are to controlled research in the rice fulgorid development chamber.Yet, have a strong impact on the progress of test and the convenience of processing because of there are problems in indoor paddy rice plantation.At present, there is following problem in the paddy rice seed method for planting that is used to set up the plant hopper population:
1) seed treatment is loaded down with trivial details, in indoor a large amount of breedings of small brown rice planthopper and winter feeding method, adopts 50 ℃ of water treatment seeds like (2008, insect knowledge) such as Ma Chongyong, needs black wool towel to preserve moisture.
2) Zhang Aimin and Liu Xiangdong (2008; Insect knowledge) short-cut method of a kind of indoor feeding small brown rice planthopper has been proposed; Though the method simple possible because rice root can penetrate in the sponge, needs constantly to change sponge; And sponge be immersed in the liquid mouldy easily, so also there is certain defective in the method.
3) more traditional kind method for planting is an earth culture, and carrying gets up to require great effort, and is prone to pollute the laboratory, and it is bad to be placed on indoor plantation daylighting, and takes up space.Handle if make test tube, the branch seedling complicacy that lifts seedlings, and be unfavorable for the insect pest situation observation.
4) flourishing if the rice seedling root system is looked inadequately, transplant seedlings or be prone to lodging when dividing seedling, be unfavorable for test operation, influence result of the test.
5) generally grow seedlings the seed soaking process is all arranged, process of the test is become complicated.
Summary of the invention
The present invention is directed to the defective that above field exists, proposed a kind of paddy rice seed method for planting that is used to set up the plant hopper population.
A kind of paddy rice seed method for planting that is used to set up the plant hopper population provided by the present invention comprises the steps:
(1) live: rice paddy seed is tiled in bottom of culture vessel, and water spray makes it moistening on said rice paddy seed, and said culture vessel is built, and prevents to become dry because of water evaporates makes seed rice;
(2) will through the rice paddy seed after above-mentioned steps (1) is handled temperature be 25 ℃-30 ℃, day and night light application time under for 14:10h and moistening condition, cultivate;
When (3) treating that the paddy rice seedling grows to 2-3cm, water nutrient solution, and insert the plant hopper population.
Said method also is included in the live preceding elder generation of said step (1) rice paddy seed is used the clear water rinsing, removes the step of floater.
Sowing described in the said step (1) is under temperature is 25 ℃-30 ℃ condition, to carry out.
The time of cultivating described in the said step (2) is 5-10d.
Nutrient solution is grouped into by the one-tenth of following concentration described in the said step (3): 0.1551g/L NH
4Cl, 0.11075g/L CaCl
2, 0.050375g/L NaH
2PO
42H
2O, 0.08925g/L K
2SO
4, 0.405g/L MgSO
47H
2O, 7.7 * 10
-3G/L FeCl
36H
2O, all the other are water.
Said plant hopper population is the population of plant hopper rice grub.
Said culture vessel is glass culture dish, glass beaker or plastic casing.
Said diameter 9cm glass culture dish, 100 left and right sides seed rice tile.
Said culture vessel method for sealing is for to seal with glass cover or preservative film; If without preservative film, when temperature was higher than 30 ℃, the dehydration symptom can appear in the paddy rice bud.
When cultivating said rice paddy seed, the every day at initial stage of germinateing is diligent observes diligent water spray, moistening for well with seed.
When said cultivation temperature was 27 ℃, said rice paddy seed one all left and right sides presidents went out flourishing root system.
After said rice seedling is higher than 2-3cm, waters 1cm deep water rice nutrient solution and can keep about a week.
The high 28*18*9cm plastic casing of said length and width is placed on illumination box and raises the plant hopper population for best.
Plant hopper is a food to draw rice seedling juice, has only growth potential paddy rice seedling preferably, could set up good plant hopper population, could required test material constantly be provided in indoor continuous raising, satisfies the demand of scientific research.Raise through long, we find that along with withering gradually of rice seedling, the plant hopper imago/nymph also can deadly thereupon perhaps be transferred on other healthy and strong rice seedlings, and therefore, the plantation of the health of rice seedling is related to the progress of whole test and the integrality of test material.Major advantage of the present invention is embodied in the following aspects:
1. the uniformity that keeps experimental condition: no matter be that the paddy rice seed is planted or the plant hopper raising, all need illumination condition,, even also have facilitation, adopt the illumination direct sowing method to save complicated operations so since illumination is little to the influence of the percentage of seedgermination under live.If the raising condition is limited, cultivation of seedling and the raising of plant hopper can be carried out synchronously, have both saved the space, have also improved the efficient of test;
2. saving funds: Ma Chongyong etc. (2008) adopt 50 ℃ of water treatment seeds in indoor a large amount of breedings of small brown rice planthopper and winter feeding method, need black wool towel to preserve moisture, and do not use nutrient solution.The young rice seedlings growth cycle that the method cultivates is short, needs duty to change seedling, also need be equipped with firing equipment or thermometer measure water temperature, and is more loaded down with trivial details.Zhang Aimin and Liu Xiangdong (2008) have proposed the short-cut method of a kind of indoor feeding small brown rice planthopper; Though the method simple possible because rice root can penetrate in the middle of the sponge, needs constantly to change sponge; And sponge be immersed in the liquid mouldy easily, so also there is certain defective in the method.
3. the method can guarantee that the plant hopper ovum normally hatches; Save time and energy: every experienced plant hopper raiser knows, white-backed planthopper lays eggs and could hatch in about 1 week of back, during the normal growth of necessary maintenance paddy rice seedling; In case seedling is withered, can cause the extinction of whole population.The ovum of plant hopper is different with the imago/nymph life habit, and it is produced in the leaf sheath of paddy rice, can not can transfer on the fresh rice seedling as imago/nymph, and rice seedling withered is directly connected to the hatching of ovum and keeping of population.The rice seedling that the illumination direct sowing method is cultivated can be grown 1 month at least, had saved the time of raising on the insect that is wasted in greatly.
4. the paddy rice seedling can not lodge, and is convenient to transplant: treat that the paddy rice seedling grows to 4-5cm, when being badly in need of seedling 2-3cm also can, this moment, the rice seedling root system was very flourishing, no matter be that the 2-3 strain is transplanted, still more strains are transplanted, and can not lodge, and make things convenient for test operation.
5. keep the laboratory clean, simplified the step of growing seedlings: the method is fit to indoor raising of seedling and indoor processing, and easy to operate health can satisfy the needs of laboratory test fully.In addition, whole test does not need seed soaking, has reduced the plantation step, has saved complicated operations.
The present invention is directed to the paddy rice planting technology has carried out repeatedly optimizing; Screen the most simple kind method for planting; For vast researcher has been saved valuable scientific research time, space and funds, carry out the technological reserve that simple possible is provided smoothly for various provincial, and municipal levels and national project.This method cost is low, saves room and time, and is simple to operate, improved the efficient of test greatly.
The advantage of this method is to keep the laboratory clean; The paddy rice seedling is healthy and strong, and well developed root system can not lodge, and is convenient to cluster and transplants; Conveniently connect worm and change worm, polypide healthy growth; Rice seedling can be grown about one month, convenient breadboard various processing operations; Lower to the planting conditions requirement, common laboratory all can be planted.This method is simple, and is especially more obvious for the not high seed effect of germination rate.It should be noted most that the duration of germination seed can not spray water too much, is main to keep rice paddy seed moistening only, wants diligent water spray every day, but can not in case the seedling dehydration is withered, be difficult to recover growth very little.Treat can water nutrient solution after the root growth prosperity, water nutrient solution this moment, and needn't to look like budding time so frequent.This method is carried out correlative study for the various plant hoppers of raising and is had very strong practicality.
Embodiment
Below in conjunction with embodiment the present invention is done further explain.
Embodiment 1, be used to set up the establishment of the paddy rice seedling novel species method for planting of plant hopper population
Used rice paddy seed kind in the present embodiment: excellent No. 8 of hybrid rice drought (Gu Fenghua etc., 2011, Shanghai Agricultural science and technology), the saving water, resisting drought rice that the Academy of Agricultural Sciences, Shanghai City gene center is cultivated, the Yin Changwen resting period is more of a specified duration, and germination rate is relatively poor; Conventional rice Jin Feng (Tang Zhongshui and Zhang Guochun, 2011, modern agriculture science and technology), the Academy of Agricultural Sciences, Shanghai City crop investigations is cultivated, and germination rate is higher.Glass beaker used herein all is internal orifice footpath diameter 10cm, height 15.3cm; Glass culture dish all is diameter 9cm, high 1.5cm.
More than used rice paddy seed hybrid rice drought with conventional rice Jin Fengben laboratory preservation is arranged all excellent No. 8, can provide to the public and be used for demonstration test.
One, low germination rate percentage of seedgermination relatively
The used rice paddy seed kind of this method is excellent No. 8 of hybrid rice drought.
Handle all 30 ℃ of constant temperature illumination boxs plantations, daytime, night, light application ratio was 14:10h.
1.1 baking and seed soaking are handled the influence of germination rate
Seed rice is put into glass beaker, (illumination is arranged) baking 12h in 40 ℃ of constant incubators.Add entry then, outwell floating flat seed, keep the high 2cm of water, (unglazed) immersion 24,48,72h in 40 ℃ of insulating boxs.3 metafiltration paper on the pad in glass culture dish, 100 seeds of handling well of each culture dish tiling are sprayed water with miniaturised nebuliser, soak with filter paper to be as the criterion, and build lid.3 repetitions are established in each processing, are placed in 30 ℃ of unglazed illumination boxs, keep rice paddy seed moistening, observe the seed sprouting situation every day.If another is treated to the seed that does not toast, other are provided with same baking processing.
The result: through observing continuously the germination of 11d, we find that every 40 ℃ of seeds that toasted all can not germinate, and 40 ℃ to be dipped to 72h mouldy smelly; Though do not have the seed of baking that indivedual germinations are arranged, germination rate is very low.40 ℃ of seeds that soak 24h, 48h began to germinate on the 3rd day, were 2% but 24h handles germination rate; It is 1% that 48h handles germination rate, germinates and all dies after 3 days; Soak 72h and began in the 7th day to germinate, germination rate is 1%, bud very a little less than, die after germinateing the 2nd day.This just explains that high temperature toasts continuously and seed soaking all is unfavorable for seed sprouting.
1.2 soaking temperature, time and illumination are to the influence of germination rate
30 ℃ and 40 ℃ of soaking temperatures, seed soaking divides illumination (L:D=14:10h) and two kinds of processing of unglazed photograph, and processing method is with 1.1, and different is all seed not to be done any processing before the seed soaking.Unglazed photograph during seed soaking is put glass culture dish and is cultivated also unglazed photograph.
Result: learn that by 1.1 40 ℃ of seed soaking 24-72h seeds germinate hardly.30 ℃ of seed soaking germination rates are higher than the germination rate of 40 ℃ of seed soaking.30 ℃ of seed soaking 24-48h 3d begin to germinate, and 72h 4d begins to germinate.Seed soaking time is long more, and it is more late to germinate.Germinating time mainly concentrated in seed soaking 1 week of back, therefore, compared germination rate with seeds germinated in 1 week.Through the statistics, under the no optical condition, different seed soaking times to percentage of seedgermination do not make significant difference (F=0.79, P=0.4953); Have under the optical condition, seed soaking time is long more, germination rate (table 2) slightly on the low side.No matter have or not illumination, the seed of immersion 24,48h begins after the 3rd day to germinate, and the seed of immersion 72h began to germinate on the 4th day.Seed soaking time is long more, and it is more late to germinate.
In general, seed soaking illumination to the germination rate influence significantly has the seed soaking percentage of seedgermination of illumination all to be higher than 5%, and there is not the seed soaking percentage of seedgermination of illumination all to be lower than 5% (table 1).
Table 130 ℃ different seed soaking times and illumination are to the influence of germination rate
| Seed soaking illumination | Seed soaking time | Germination rate % |
| Do not have | 24h | 4.33±1.45c |
| 48h | 2.67±0.67c | |
| 72h | 4.00±0.58c | |
| Average | 3.67±0.55B | |
| Have | 24h | 13.67±2.96a |
| 48h | 10.67±0.88ab | |
| 72h | 7.33±0.33bc | |
| Average | 10.55±1.28A |
Lowercase is represented the difference of each seed soaking time germination rate, and capitalization representes to have or not the difference of illumination germination rate.
Institute's marking-up mother is a Duncan ' s multiple range test average comparative result in the table, and analysis level is 0.05.
1.3 it is live with sowing germination rate relatively behind the seed soaking 48h
Pad 3 metafiltration paper in glass culture dish, 100 seeds of each culture dish tiling are not done any processing to seed before the tiling and are live.With miniaturised nebuliser water spray, soak with filter paper and to be as the criterion, build lid, 3 repetitions are established in each processing, are placed in 30 ℃ of unglazed illumination boxs, and spray water once every day, observation seed sprouting situation.Sowing is handled and has been optical condition behind the seed soaking 48h, and other are provided with unglazed live.
The result: 30 ℃ of live seed sproutings early promptly had indivedual seed sproutings, and had indivedual seed sproutings and sow the 3rd talent again after the seed soaking in after planting the 2nd day.Though different seeding methods can influence germination sooner or later, live germination rate is slightly higher than seed soaking back sowing germination rate, and both germination rates do not have significant difference (F=2.57, P=0.1841) (table 2).
It is live with the influence of the illumination seed soaking unglazed sowing in back to germination rate that table 230 is ℃ unglazed
| Seeding method | Germination rate % |
| Unglazed live | 17.67±2.19a |
| Unglazed sowing behind the illumination seed soaking 48h | 13.67±1.20a |
With table 1.
1.4 illumination condition is to the influence of live germination rate
Type of seeding is established two processing of illumination (L:D=14:10h) and unglazed photograph with 1.3.
The result: (live germination rate is a little more than the germination rate under the no optical condition under the illumination condition for F=1.16, P=0.3414) (table 3) though illumination condition does not make significant difference to live germination rate; There is the young shoot growth potential of illumination relatively good; Miao Zhuan, green, and the unglazed young shoot that shines condition turns white, a little less than the seedling.
Table 3 illumination condition is to the influence of live germination rate
| Live illumination | Germination rate % |
| Have | 17.67±2.19a |
| Do not have | 15.00±1.15a |
With table 1.
1.5 live and water is broadcast the comparison to the percentage of seedgermination influence
Type of seeding is with 1.3, and water is broadcast promptly in the culture dish filled with water, lets seed is under water to germinate, and changes inferior water at a distance from two days, prevents that the seed that rots is smelly.
The result: live the 2nd day seed begins to germinate, and water is broadcast germination in the 3rd day.Live and water are broadcast and percentage of seedgermination are influenced significant difference (F=10.32, P=0.0325), live percentage of seedgermination is significantly sowed sub-germination rate (table 4) greater than water.
Lively under table 4 illumination condition broadcast influence to percentage of seedgermination with water
| Seeding method | Germination rate % |
| Illumination is live | 11.67±0.88a |
| Illumination water is dialled | 7.00±1.15b |
With table 1.
Two, high germination rate percentage of seedgermination relatively
The used rice paddy seed kind of this method is conventional rice Jin Feng.
2.1 live illumination and long-time seed soaking, immersed depth are to the influence of percentage of seedgermination
Handle 1: pad 3 metafiltration paper in glass culture dish, 100 seeds of handling well of each culture dish tiling are sprayed water with miniaturised nebuliser; Soak with filter paper and to be as the criterion; Build lid, 3 repetitions are established in each processing, are placed in 27 ℃ of illumination boxs (L:D=14:10h); Spray water once every day, observes the seed sprouting situation;
Handle 2 with handling 1, different is unglazed photograph;
Handle 3 culture dish topped up with water, soak the cultivation of preserving moisture after 2 days;
Handle also topped up with water of 4 culture dishes, soak always and observe germination, long like fruit bud to flushing with culture dish, change glass beaker and cultivate.
The result: handling had indivedual seed sproutings after 1 day, and chitting piece is more after 2 days, and germinative number is more and more afterwards, in the germination rate maximum set at 8d.
Germination rate significant difference between each is handled after 2 days (F=13.96, P 0.0001), live germination rate is significantly higher than the immersion germination rate; Germination rate significant difference between each is handled after 3 days (F=10.29, P=0.0002), the short germination rate of soaking time is equal to live germination rate; (F=7.71, P=0.0008), the germination rate of immersion almost was equal to live germination rate (table 5) to the germination rate significant difference between each was handled after 4 days.
Every seed is immersed in the water always, and germinating time is all late than the processing of not soaking seed or seed soaking time is short, and the 2nd day germination rate is also low.When after soaking 2 days seed sowing, germination rate is near unsoaked seed within 1 day.After the 4th day, germination rate surpasses 80% basically.The 3rd day tender shoots is clearly longer, growing way: have the light live>unglazed live to soak 2d live long soaking, there is the relative root of the live bud of light longer, turn green; Unglazed live bud, root are white, and root is relatively more flourishing, be longer than bud; Soak the 2d bud and turn to be yellow slightly, root is unglazed relatively lively will lack; Long soaking handled the tender point that exposes white, and other processing are very short relatively.
The seed that immersion is handled, the 8th sky and water are smelly a bit, also have indivedual seeds to be in germination and emit the white point stage.Some bud greening that immersed depth is shallow begins to take root; The dark elongated white bud that soaks, unrooted.Influence is not too obvious to germination rate for the soaking time and the degree of depth, but can influence the morning and evening of germination and the quality of bud.
The live illumination of table 5 and long-time seed soaking, immersed depth are to the influence of percentage of seedgermination
| Seeding method | Illumination | Seed soaking time d | Depth of water cm | 2d germination rate % | 3d germination rate % | 4d germination rate % |
| Live | Have | 0 | 0 | 13.33±1.20a | 67.67±1.86ab | 79.33±0.67c |
| Live | Do not have | 0 | 0 | 13.00±0.58a | 69.67±2.60a | 82.00±1.00b |
| Soak afterwards earlier and broadcast | Have | 2 | 1 | 5.33±0.88b | 70.67±0.33a | 89.00±0.58a |
| Long soaking | Have | 8 | 1 | 5.33±0.33b | 48.00±1.00d | 81.00±0.58b |
| Long soaking | Have | 8 | 2.5 | 4.33±0.33b | 59.33±4.81c | 72.00±3.46c |
| Long soaking | Have | 8 | 5 | 5.67±0.33b | 60.33±1.67bc | 75.67±1.86bc |
| Long soaking | Have | 8 | 7.5 | 5.33±2.19b | 61.67±2.19bc | 80.67±2.85b |
With table 1.
2.2 it is live with the influence of short time seed soaking back sowing to germination rate
Be optical condition, every culture dish is put 100 seeds, adds 1cm water, soaks 1/20,2,4,6,8,24 respectively, 48h, and each handles repetition 3 times.The culture dish that tiles is afterwards observed germination, sprays low amounts of water every day, and is moistening for fitting with seed, observes germination.
The result: through Duncan relatively, (F=5.35, P=0.0046), the 2nd day germination rate of seed that soaks 48h is minimum for the 2nd day germination rate significant difference.The 5th day germination rate difference not significantly (F=1.59, P=0.2212), the 6th day germination rate difference not significantly (F=1.90, P=0.1514).The 2nd day begins the point that shows money or valuables one carries unintentionally, and seed soaking time is of a specified duration more, and the sharp rate that shows money or valuables one carries unintentionally is low more; Germination rate difference was no longer remarkable greater than 80% the seed point that all shows money or valuables one carries unintentionally in the 3rd day, until 5-6 days, the bud difference in height is little.Live germination rate did not almost have difference with seed soaking back sowing germination rate after this had just explained the rinsing seed, can save the complex steps breeding (table 6) of seed soaking.
Table 6 illumination is live with the influence of short time seed soaking back sowing to germination rate
| Seed soaking time h | 2d germination rate % | 5d germination rate % | 6d germination rate % |
| 1/20 | 22.67±1.76b | 94.67±1.86ab | 94.33±1.67ab |
| 2 | 30.67±3.18a | 95.33±1.67ab | 94.67±1.45ab |
| 4 | 28.33±2.03ab | 96.67±1.33a | 96.67±1.33a |
| 6 | 22.33±1.20b | 96.00±0.58ab | 96.00±0.58ab |
| 8 | 25.67±4.41ab | 97.33±1.45a | 97.33±1.45a |
| 24 | 27.33±0.88ab | 92.33±0.33b | 92.33±0.33b |
| 48 | 14.00±0.58c | 95.00±1.00ab | 95.00±1.00ab |
With table 1.
Through a plurality of testing and verifications: the live rice paddy seed growing way of illumination is best, and germination rate is also higher, and simply saves trouble.Flourishing seedlings root makes the seedling cluster transplant and is not easy lodging.And healthy rice growth, long-term nutrition liquid is cultivated and also is not easy mouldy fouling, has guaranteed the cleaning growth of rice seedling.Treat that seedling grows to the 2-3cm height, the pouring nutrient solution just can insert plant hopper, is convenient to the indoor feeding of rice fulgorid population.The illumination direct sowing method is different from traditional soil and broadcasts method, also is different from the tank farming of a small amount of result of study, and this method is a kind of improvement at additive method.
Embodiment 2, the indoor paddy rice seedling novel species method for planting of setting up the plant hopper population
One, is used to set up the paddy rice seedling novel species method for planting of plant hopper population
1) earlier rice paddy seed is used the clear water rinsing before the sowing, remove floater;
2) live: pad 3 metafiltration paper (also can not fill up, the filter paper moistening effect is better) in glass culture dish or other transparent plastic containers, bottom tiling one deck seed; With the miniaturised nebuliser water spray, moistening with seed for well, cover the culture dish lid; Plastic containers can be used the preservative film cover, with sprayer water spray around container, guarantee that preservative film is close on the chamber wall before the cover; Prevent water evaporates, seed rice becomes dry.Plastic containers are fit to grow seedlings on a large scale, and moistening effect is better than glass culture dish.This step most critical waters dilutional hyponatremia and is unfavorable for the rice seedling root growth, and is prone to mouldy fouling.
3) seed of handling well is placed in 27 ℃ of illumination boxs or the seedling rearing room, day and night, the light application time ratio was 14:10h, observed germination and water spray every day and kept moistening.
4) cultivate 1 all backs rice seedling and can grow to 2-3cm or higher, this moment, well developed root system watered nutrient solution, can insert different plant hopper populations and do different test processing.
Said nutrient solution is by the species composition of following concentration: 0.1551g/L NH
4Cl, 0.11075g/L CaCl
2, 0.050375g/L NaH
2PO
42H
2O, 0.08925g/L K
2SO
4, 0.405g/L MgSO
47H
2O, 7.7 * 10
-3G/L FeCl
36H
2O, all the other are water.
Two, white-backed planthopper is in the survival of cultivating on the rice seedling
With highly placing 1000ml glass beaker or other plastic wares for the paddy rice seedling of 4-5cm; Water the dark nutrient solution of 1cm; Connect the white-backed planthopper imago/nymph that the field collects, be placed on open continuous raising in 27 ℃ of illumination boxs, the population of foundation supplies other tests to handle usefulness.Find that rice seedling has the flavescence symptom, renew seedling.Change the time of seedling and how much decide general sustainable about 1 month according to what worm was measured.
With pest sucking device with 5 age nymph receive in the 250cm plastic cup; The back male and female adult of waiting to sprout wings matches; In the 1000ml glass beaker, lay eggs, it is long that the single worm in ovum hatching back is received 20cm, in the internal diameter 2.2cm teat glass; All put the 4-5cm height paddy rice seedling 2-3 strain of cultivation in each container in advance, observe nymph survival rate, development duration, adult eclosion rate and life-span (table 7).
The result: the white-backed planthopper imago/nymph all can the subculture survival on the paddy rice seedling of cultivating.The survival rate of nymph is more than 90%, development duration 11-14 days; The adult eclosion rate is more than 90%, life-span 6-32d.Because of brown planthopper similar with hazard profile with small brown rice planthopper with the form of white-backed planthopper, so the rice seedling breeding method is equally applicable to this two kinds of plant hoppers.
Table 7 white-backed planthopper is compared in the survival of cultivating on the rice seedling
Three, conventional raising method is set up plant hopper population effect relatively with raising method of the present invention
Zhang Aimin and Liu Xiangdong (2010) (insect knowledge; 45 (2): 314-316) having introduced a kind of is that medium is cultivated rice seedling with the sponge; Method of raising small brown rice planthopper in enormous quantities, concrete grammar is following: rice paddy seed is tiled in the plastic casing that is lined with sponge after 24h soaks.Under 26 ℃, it is high that 10d left and right sides rice seedling can grow to 6～8cm, is used to raise small brown rice planthopper this moment.Rice seedling can keep growing way preferably, about sustainable 30d.
The single head of small brown rice planthopper is raised and is adopted single seedling single tube method for breeding, and the small brown rice planthopper nymph survival rate that this method is raised is all more than 80%, and development duration is about 14d, and the small brown rice planthopper generation time is 20～35d.It is little to compare difference with the survival of table 7 white-backed planthopper of the present invention, but paddy rice planting method of the present invention is more simple.
Adopt method of the present invention the indoor feeding white-backed planthopper more than 2 years, bred by the hundreds of head of beginning and be thousands of imago/nymphs, therefore, other plant hopper population method for building up relatively, this method is a kind of more simple, practicable method.
Claims (5)
1. a paddy rice seed method for planting that is used to set up the plant hopper population comprises the steps:
(1) live: rice paddy seed is tiled in bottom of culture vessel, and water spray makes it moistening on said rice paddy seed, and said culture vessel is built, and prevents to become dry because of water evaporates makes seed rice;
(2) will through the rice paddy seed after above-mentioned steps (1) is handled temperature be 25 ℃-30 ℃, day and night light application time under for 14:10h and moistening condition, cultivate;
When (3) treating that the paddy rice seedling grows to 2-3cm, water nutrient solution, and insert the plant hopper population.
2. method according to claim 1 is characterized in that:
Said method also is included in the live preceding elder generation of said step (1) rice paddy seed is used the clear water rinsing, removes the step of floater.
3. method according to claim 1 is characterized in that:
Sowing described in the said step (1) is under temperature is 25 ℃-30 ℃ condition, to carry out.
4. method according to claim 1 is characterized in that:
The time of cultivating described in the said step (2) is 5-10 days.
5. method according to claim 1 is characterized in that:
Nutrient solution is grouped into by the one-tenth of following concentration described in the said step (3): 0.1551g/L NH
4Cl, 0.11075g/L CaCl
2, 0.050375g/L NaH
2PO
42H
2O, 0.08925g/L K
2SO
4, 0.405g/L MgSO
47H
2O, 7.7 * 10
-3G/L FeCl
36H
2O, all the other are water.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106561216A (en) * | 2016-10-29 | 2017-04-19 | 施秉县富民高新农业发展有限公司 | Planting method for improving yield of leguminosae |
| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561216A (en) * | 2016-10-29 | 2017-04-19 | 施秉县富民高新农业发展有限公司 | Planting method for improving yield of leguminosae |
| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
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