CN106305632A - Large-scale breeding method for laodelphax striatellus - Google Patents
Large-scale breeding method for laodelphax striatellus Download PDFInfo
- Publication number
- CN106305632A CN106305632A CN201610743669.9A CN201610743669A CN106305632A CN 106305632 A CN106305632 A CN 106305632A CN 201610743669 A CN201610743669 A CN 201610743669A CN 106305632 A CN106305632 A CN 106305632A
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- wheat
- brown rice
- small brown
- rice planthopper
- worm cover
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- 238000009395 breeding Methods 0.000 title claims abstract description 23
- 241001470017 Laodelphax striatella Species 0.000 title abstract 8
- 241000209140 Triticum Species 0.000 claims abstract description 41
- 235000021307 Triticum Nutrition 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000009331 sowing Methods 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 6
- 230000035784 germination Effects 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- 235000021329 brown rice Nutrition 0.000 claims description 69
- 241001498622 Cixius wagneri Species 0.000 claims description 68
- 239000002689 soil Substances 0.000 claims description 28
- 235000016709 nutrition Nutrition 0.000 claims description 16
- 230000035764 nutrition Effects 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 12
- 210000000582 semen Anatomy 0.000 claims description 12
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 238000007664 blowing Methods 0.000 claims description 4
- UHZZMRAGKVHANO-UHFFFAOYSA-M chlormequat chloride Chemical compound [Cl-].C[N+](C)(C)CCCl UHZZMRAGKVHANO-UHFFFAOYSA-M 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 4
- 241000819999 Nymphes Species 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims 2
- 201000010099 disease Diseases 0.000 abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 12
- 230000001488 breeding effect Effects 0.000 abstract description 9
- 241000700605 Viruses Species 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract 5
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 235000009973 maize Nutrition 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 208000036142 Viral infection Diseases 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001466042 Fulgoromorpha Species 0.000 description 1
- 241000702659 Maize rough dwarf virus Species 0.000 description 1
- 241000209094 Oryza Species 0.000 description 1
- 208000031481 Pathologic Constriction Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000004047 hole gas Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a large-scale breeding method for laodelphax striatellus by utilizing wheat as main feed, and belongs to the technical field of insect breeding. The large-scale breeding method comprises the following steps: screening wheat seeds and accelerating germination of the wheat seeds, sowing the germinated wheat seeds in a green house, and taking the wheat seeds as laodelphax striatellus feeding feed when wheat grows to the two-leaf one-heart period, and is 6cm high; and covering the wheat by an insect breeding cover, and breeding laodelphax striatellus on wheat seedlings covered by the insect breeding cover. According to the large-scale breeding method for the laodelphax striatellus provided by the invention, a method which takes wheat as feed to carry out laodelphax striatellus annual breeding is gradually formed based on multi-year research on diseases infected with viruses transmitted by insects such as the maize rough dwarf disease, the wheat rosette dwarf and the wheat yellow-stripe dwarf disease. The large-scale breeding method is simple and convenient to operate, guarantees consistent larva instar and a high laodelphax striatellus breeding rate, realizes annular continuous feeding for a large number of laodelphax striatellus, can provide enough test insects at any time, and meets experimental needs of many units.
Description
Technical field
The present invention relates to one utilizes Semen Tritici aestivi to carry out small brown rice planthopper large-scale breeding method for main feed, belongs to insecticide and raises skill
Art field.
Background technology
Small brown rice planthopper is a class primary pest of serious harm gramineous crop, is distributed widely in China various places.Semen Tritici aestivi, jade
Rice, Oryza sativa L. are the staple food crops of northern China, and every kind of crop all has generation and the harm of multiple virosis.As: Semen Maydis
Rough dwarf disease, stripe disease, wheat rosette stunt, Wheat Yellow striped dwarf wilt etc., and small brown rice planthopper is above-mentioned important virosis
Main vector.In recent years, along with the impact of the factor such as cropping system and Climate Anomalies, the disease that small brown rice planthopper causes
Virus disease occurs in that new variation tendency.Such as the seventies in last century, the wheat rosette stunt of big generation in Hebei province, current field
Between be fragmentary generation, and the new virus disease that Wheat Yellow striped dwarf wilt is propagated as a kind of small brown rice planthopper is little in China at present
Wheat producing region occurs universal.These changes of virosis, raise new difficuties to field diseases preventing and treating.The preventing and treating that arbovirus is sick,
Clearly pass the harmful characteristics of virus mediator itself, the biography poison characteristic of amboceptor, host and the interaction of plant, screen disease-resistant variety, screening
Pesticide control is the main contents of arbovirus disease research, and the basis carrying out the studies above is then the indoor scale of small brown rice planthopper
Batch is raised.Therefore, provide valid period of standard test with small brown rice planthopper in the anniversary in a large number, be by the basis of the sick every research of arbovirus
And premise.Document announcement artificial breeding small brown rice planthopper generally utilizes Oryza sativa L. as feedstuff, but paddy growth is to illumination, moisture content, temperature
Require higher, be unfavorable for that the north is raised in the anniversary on a large scale.
Summary of the invention
For above-mentioned technical problem, the present invention provides a kind of small brown rice planthopper large-scale breeding method, and Semen Tritici aestivi is as small brown rice planthopper
Suitable existence and host egg, do not limited by environmental condition.
The concrete technical scheme that the present invention takes is:
Small brown rice planthopper large-scale breeding method, including procedure below:
(1), the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: fetch soil to field test and sieve, then spends soil, Vermiculitum with volume ratio 2:1 with commercially available:
The ratio of 1 prepares Nutrition Soil, standby;
Wheat seed screening, accelerating germination: selecting blade generous, plant type is compact, the wheat breed of growing way stalwartness is as feedstuff;Choosing
Take the seed that plumpness is consistent, be soaked in chlorocholine chloride: in the soak that clear water is converted with the ratio of volume 1:25, after soaking 24h,
Repeatedly rinsing well with clear water, drain moisture, surface covers the gauze soaked and carries out moisturizing, rinses seed once every 12h,
Sowing i.e. it is ready for after seed shows money or valuables one carries unintentionally;
Sowing in greenhouse: cut-off footpath 10cm, the flowerpot of degree of depth 10cm, fill the Nutrition Soil of 2/3 in flowerpot, surface is watered
Water, after water fully penetrates into soil, rushes to the wheat seed sprouted with sprouting, and root mode down is uniformly seeded in
In flowerpot, covering flowerpot surface, then spread last layer Nutrition Soil at the surface of the seed, thickness is advisable to cover seed, compacting;Treat little
Wheat length, to 2 leaf 1 heart stages, when highly reaching 6cm, takes food feedstuff as small brown rice planthopper;
(2), the raising of small brown rice planthopper:
With foster worm cover, the Semen Tritici aestivi in flowerpot is clasped, small brown rice planthopper is raised on the Caulis et Folium Tritici aestivi that foster worm cover clasps, foster worm
The outside of cover is plugged label, labelling feeding time, changes feedstuff 1 time every 6-7 days;
Foster worm cover herein is divided into 2 different sizes, raises the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is used for raising
Support 1-3 small brown rice planthopper in age;40 mesh gauzes are for above small brown rice planthopper in raising 4 age;
Insectary environmental condition is: temperature 23 ± 2 DEG C, relative humidity 65%~75%, illumination 14h: D dark 10h.
Wherein, change feedstuff method: open foster worm cover bottom side, blow bottom the foster worm cover let drive at out with inflation pumping hole
Gas, blows to small brown rice planthopper support worm cover top, after blowing clean, makes to support worm cover top down, it is to avoid small brown rice planthopper jumps out, and then rests worm
Cover covers on new wheatfeed together with small brown rice planthopper, and outside label labelling changes the Seedling date, completes to change Seedling process.
No on the most used wheatfeed inspection plant have ovum, and without ovum, the most used feedstuff pulls out, and soil falls
Going out, the Posterior circle that sieves uses;If there being ovum on plant, sprinkling the wheat seed that plumpness is consistent, earthing in the gap of wheat seedling, watering
Water, buckles well foster worm cover, record date, and this process is referred to as sowing relay wheat;Small brown rice planthopper hatching after 10-13 days, relay wheat is
Long to 2 leaf 1 hearts, become the new feedstuff hatching nymph.
The small brown rice planthopper large-scale breeding method that the present invention provides, its feature is:
1, preparation Nutrition Soil, needs field normal soil: fertilizers for potted flowers Nutrition Soil: Vermiculitum is 2:1:1, makes Nutrition Soil moisture retention more
Good, nutrition is more rich.
2, the soak for Semen Tritici aestivi accelerating germination is: chlorocholine chloride: the soak that clear water is converted according to the ratio of 1:25, makes Semen Tritici aestivi
After sprouting, growing way small brown rice planthopper sturdy, preferably takes food.
When 3, changing small brown rice planthopper feedstuff, small brown rice planthopper is blown to support the top of worm cover by the gas utilizing inflator pump to blow out, and reduces
The difficulty of manpower purging, saves the time compared with using brush pen transfer small brown rice planthopper, considerably increases transfer efficiency.Small brown rice planthopper
Large-scale breeding needs to change more feedstuff every day, utilize inflator pump can make whole feeding process save substantial amounts of artificial and
Time.
When 4, the small brown rice planthopper that laid eggs being changed feedstuff, sow relay wheat, it is ensured that the raising in small brown rice planthopper time of infertility,
And owing to small brown rice planthopper likes taking food the plant that children is tender, in small brown rice planthopper feeding process, omnidistance use children's tender wheat Seedling as feedstuff,
I.e. add small brown rice planthopper spawning rate, improve again incubation rate and survival rate.
5, small brown rice planthopper raising whole process is carried out in the insectary of 15, high insulating effect, the air conditioner of built-in 35W, entirely
Year, temperature control ability was good, and foster worm frame is all propped up by rustless steel and constitutes, and support effect is obvious.Cantilever tip be mounted with plant growth lamp and
Intervalometer, it is ensured that Semen Tritici aestivi and the required illumination condition of small brown rice planthopper growth.
6, the small brown rice planthopper consistent in order to obtain development duration, lays eggs beginning from small brown rice planthopper, changes 1 feedstuff every 3 days, with
Time sowing relay wheat, with a collection of small brown rice planthopper larva hatched when changing Seedling not larva with spawning and hatching in other in period mix.
So ensure that the small brown rice planthopper age in same foster worm cover is consistent.
Detailed description of the invention
It is described in conjunction with the embodiments the detailed description of the invention of the present invention.
1, the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: fetch soil to field test and sieve, sieve removes the bulky grains such as stone, then buys back with market
Soil, Vermiculitum is spent to prepare Nutrition Soil with the ratio of 2:1:1, standby.
Wheat seed screening, accelerating germination: selecting blade generous, plant type is compact, the wheat breed of growing way stalwartness is as feedstuff.Really
After determining wheat breed, choose the seed that plumpness is consistent, be soaked in chlorocholine chloride: the soak that clear water is converted with the ratio of 1:25
In, after soaking 24h, repeatedly to rinse well with clear water, drain moisture, surface covers the gauze soaked and carries out moisturizing, every 12h
Rinse seed once, after seed shows money or valuables one carries unintentionally, be i.e. ready for sowing.
Sowing: cut-off footpath 10cm, the flowerpot of degree of depth 10cm, fills the Nutrition Soil of 2/3 in flowerpot, and watering in surface, treats water
After fully penetrating into soil, the wheat seed sprouted is rushed to sprouting, root mode down, is uniformly seeded in flowerpot,
Covering flowerpot surface (about 250), then spread last layer Nutrition Soil at the surface of the seed, thickness is advisable to cover seed, compacting.Treat
Semen Tritici aestivi length is to 2 leaf 1 heart stages, the most about during 6cm, can take food feedstuff as small brown rice planthopper.
Above procedure is carried out in greenhouse.
2, the raising of small brown rice planthopper:
Semen Tritici aestivi in flowerpot is clasped, by small brown rice planthopper by the foster worm cover made with diameter 9cm white clear acrylic pipe+gauze
Raising on the Caulis et Folium Tritici aestivi that foster worm cover clasps, support worm cover upper end gauze and clasp, rubber band is tied tight.It is plugged in the outside of foster worm cover
Label, labelling feeding time.Feedstuff is changed 1 time every 6-7 days.
Foster worm cover herein is divided into 2 different sizes, raises the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is used for raising
Support 1-3 small brown rice planthopper in age;40 mesh gauzes are for above small brown rice planthopper in raising 4 age.
Small brown rice planthopper changes feedstuff method: carefully opens foster worm cover bottom side, opens inflator pump, let drive at by inflation pumping hole
The foster worm cover bottom blowing opened, blows to small brown rice planthopper support worm cover top, after blowing clean, makes to support worm cover top down, it is to avoid ash flies
Louse jumps out, and then rests worm cover and covers on new wheatfeed together with small brown rice planthopper, and outside label labelling changes the Seedling date, completes to change Seedling
Process.No on the most used wheatfeed inspection plant have ovum, and without ovum, the most used feedstuff pulls out, and soil is poured out,
The Posterior circle that sieves uses.If there being ovum on plant, the most used feedstuff does not abandons, and sprinkles plumpness in the gap of wheat seedling consistent
Wheat seed, earthing, to water, buckle well foster worm cover, record date, this process is referred to as sowing relay wheat.After 10-13 days, ash flies
Louse hatches, and relay wheat has grown to 2 leaf 1 hearts, becomes the new feedstuff hatching nymph.
Small brown rice planthopper raise in the insectary of 15, insectary environmental condition is: temperature 23 ± 2 DEG C, relative humidity (70 ±
5) %, illumination 14h: dark 10h.
The small brown rice planthopper large-scale breeding method that the present invention provides, is to MRDV, wheat rosette stunt, Wheat Yellow bar
On the basis of the arbovirus diseases such as stricture of vagina dwarf wilt are studied for many years, gradually form a set of using Semen Tritici aestivi as feedstuff, carried out small brown rice planthopper
The method that anniversary raises.The method is easy and simple to handle, and worm is consistent for age, and small brown rice planthopper breeding potential is high, it is achieved that the whole year of a large amount of small brown rice planthoppers
Raise continuously, enough test can be provided at any time with worm, meet the experiment needs of many units.
Claims (3)
1. small brown rice planthopper large-scale breeding method, it is characterised in that include procedure below:
(1), the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: fetch soil to field test and sieve, then spends soil, Vermiculitum with volume ratio 2:1:1 with commercially available
Ratio prepares Nutrition Soil, standby;
Wheat seed screening, accelerating germination: selecting blade generous, plant type is compact, the wheat breed of growing way stalwartness is as feedstuff;Choose full
The seed that full scale is consistent, is soaked in chlorocholine chloride: in the soak that clear water is converted with the ratio of volume 1:25, after soaking 24h, with clear
Water is rinsed well repeatedly, drains moisture, and surface covers the gauze soaked and carries out moisturizing, rinses seed once every 12h, waits to plant
Son is i.e. ready for sowing after showing money or valuables one carries unintentionally;
Sowing in greenhouse: cut-off footpath 10cm, the flowerpot of degree of depth 10cm, fill the Nutrition Soil of 2/3 in flowerpot, waters in surface,
After water fully penetrates into soil, the wheat seed sprouted is rushed to sprouting, root mode down, be uniformly seeded in flowerpot
In, covering flowerpot surface, then spread last layer Nutrition Soil at the surface of the seed, thickness is advisable to cover seed, compacting;Treat that Semen Tritici aestivi is long
To 2 leaf 1 heart stages, when highly reaching 6cm, take food feedstuff as small brown rice planthopper;
(2), the raising of small brown rice planthopper:
With foster worm cover, the Semen Tritici aestivi in flowerpot is clasped, small brown rice planthopper is raised on the Caulis et Folium Tritici aestivi that foster worm cover clasps, at foster worm cover
Outside is plugged label, labelling feeding time, changes feedstuff 1 time every 6-7 days;
Foster worm cover herein is divided into 2 different sizes, raises the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is used for raising 1-
3 age small brown rice planthopper;40 mesh gauzes are for above small brown rice planthopper in raising 4 age;
Insectary environmental condition is: temperature 23 ± 2 DEG C, relative humidity 65%~75%, illumination 14h: dark 10h.
Small brown rice planthopper large-scale breeding method the most according to claim 1, it is characterised in that change feedstuff method: open foster
Worm cover bottom side, the foster worm cover bottom blowing let drive at out with inflation pumping hole, blow to small brown rice planthopper support worm cover top, blow clean
After, make to support worm cover top down, it is to avoid small brown rice planthopper jumps out, and then rests worm cover and covers on new wheatfeed together with small brown rice planthopper,
Outside label labelling changes the Seedling date, completes to change Seedling process.
Small brown rice planthopper large-scale breeding method the most according to claim 1, it is characterised in that the most used wheatfeed is examined
Looking into no on plant has ovum, and without ovum, the most used feedstuff pulls out, and soil is poured out, and the Posterior circle that sieves uses;If having on plant
Ovum, sprinkles the wheat seed that plumpness is consistent, earthing in the gap of wheat seedling, waters, and buckles well foster worm cover, record date, this
Process is referred to as sowing relay wheat;Small brown rice planthopper hatching after 10-13 days, relay wheat has grown to 2 leaf 1 hearts, has become new hatching nymph
Feedstuff.
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Cited By (5)
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CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
USD841898S1 (en) | 2017-04-13 | 2019-02-26 | Aspire Food Group USA Inc. | Insect habitat stacker |
US10638788B2 (en) | 2016-05-04 | 2020-05-05 | Aspire Food Group USA Inc. | Method and system for spray drying insects |
US11395474B2 (en) | 2016-05-10 | 2022-07-26 | Aspire Food Group Ltd | Harvesting and incubating systems for cultivation of insects |
CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10638788B2 (en) | 2016-05-04 | 2020-05-05 | Aspire Food Group USA Inc. | Method and system for spray drying insects |
US11026439B2 (en) | 2016-05-04 | 2021-06-08 | Aspire Food Group Ltd | Method and system for spray drying insects |
US11395474B2 (en) | 2016-05-10 | 2022-07-26 | Aspire Food Group Ltd | Harvesting and incubating systems for cultivation of insects |
USD841898S1 (en) | 2017-04-13 | 2019-02-26 | Aspire Food Group USA Inc. | Insect habitat stacker |
CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
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