CN106305632B - Small brown rice planthopper large-scale breeding method - Google Patents
Small brown rice planthopper large-scale breeding method Download PDFInfo
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- CN106305632B CN106305632B CN201610743669.9A CN201610743669A CN106305632B CN 106305632 B CN106305632 B CN 106305632B CN 201610743669 A CN201610743669 A CN 201610743669A CN 106305632 B CN106305632 B CN 106305632B
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- 235000021329 brown rice Nutrition 0.000 title claims abstract description 78
- 241001498622 Cixius wagneri Species 0.000 title claims abstract description 75
- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 241000209140 Triticum Species 0.000 claims abstract description 52
- 235000021307 Triticum Nutrition 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 21
- 238000012216 screening Methods 0.000 claims abstract description 5
- 241001466042 Fulgoromorpha Species 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 28
- 235000016709 nutrition Nutrition 0.000 claims description 16
- 230000035764 nutrition Effects 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 238000009331 sowing Methods 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 238000011010 flushing procedure Methods 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 238000007664 blowing Methods 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 241000819999 Nymphes Species 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 230000009191 jumping Effects 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 12
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 240000008042 Zea mays Species 0.000 abstract description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 3
- 235000005822 corn Nutrition 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000209094 Oryza Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 208000036142 Viral infection Diseases 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001674048 Phthiraptera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
It is that main feed carries out small brown rice planthopper large-scale breeding method that the present invention relates to a kind of using wheat, belongs to technical field of insect feeding.Wheat seed screening, vernalization, are sowed in the greenhouse, long to 1 heart stage of 2 leaf to wheat, when highly reaching 6cm, as small brown rice planthopper feeding feed；Wheat is clasped with feeding worm cover, by small brown rice planthopper raising on the wheat seedling that feeding worm cover clasps.Small brown rice planthopper large-scale breeding method provided by the invention; it is on the basis of being studied for many years the arbovirus such as corn rough dwarf, wheat rosette stunt, Wheat Yellow striped dwarf wilt disease; a set of using wheat as feed, to carry out the raising of small brown rice planthopper anniversary method is gradually formed.This method is easy to operate, and worm age is consistent, small brown rice planthopper breeding potential height, realizes the whole year continuous raising of a large amount of small brown rice planthoppers, can provide enough tests at any time and use worm, meet the experiment needs of more units.
Description
Technical field
It is that main feed carries out small brown rice planthopper large-scale breeding method that the present invention relates to a kind of using wheat, belongs to insect raising skill
Art field.
Background technique
Small brown rice planthopper is a kind of primary pest for seriously endangering gramineous crop, is distributed widely in China various regions.Wheat, jade
Rice, rice are the staple food crops of northern China, and there are many generations and harm of virosis on every kind of crop.Such as: corn
Rough dwarf disease, stripe disease, wheat rosette stunt, Wheat Yellow striped dwarf wilt etc., and small brown rice planthopper is above-mentioned important virosis
Main vector.In recent years, with the influence of the factors such as cropping system and Climate Anomalies, disease caused by small brown rice planthopper
There is new variation tendency in virus disease.Such as last century the seventies, in the wheat rosette stunt of the big generation in Hebei province, field at present
Between be sporadicly to occur, and the new virus disease that Wheat Yellow striped dwarf wilt is propagated as a kind of small brown rice planthopper is small in China at present
Wheat producing region occurs universal.These variations of virosis, raise new difficuties to field diseases prevention and treatment.The prevention and treatment of arbovirus disease,
The harmful characteristics of virus mediator itself, the biography poison characteristic of mediator are clearly passed, the interaction of host and plant screen disease-resistant variety, screening
Pesticide control is the main contents of arbovirus disease research, and the basis for carrying out the studies above is then the indoor scale of small brown rice planthopper
Batch is raised.Therefore, valid period of standard test is largely provided in the anniversary and uses small brown rice planthopper, is the basis for carrying out the every research of arbovirus disease
And premise.Document announcement artificial feeding small brown rice planthopper is usually using rice as feed, but paddy growth is to illumination, moisture content, temperature
It is more demanding, it is unfavorable for raising on a large scale in the northern anniversary.
Summary of the invention
In view of the above technical problems, the present invention provides a kind of small brown rice planthopper large-scale breeding method, wheat as small brown rice planthopper most
It is suitable for existence and host egg, is not limited by environmental condition.
The specific technical solution that the present invention takes is:
Small brown rice planthopper large-scale breeding method, including following procedure:
(1), the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: fetching to field trial soil sieving, then with commercially available flower soil, vermiculite with volume ratio 2:1:
1 ratio prepares Nutrition Soil, spare；
Wheat seed screening, vernalization: selection blade is generous, and plant type is compact, and the wheat breed of growing way stalwartness is as feed；Choosing
The consistent seed of plumpness is taken, cycocel is soaked in: in the soak that clear water has been converted with the ratio of volume 1:25, after impregnating for 24 hours,
It is clean with clear water repeated flushing, moisture is drained, the gauze that surface covering has been soaked carries out moisturizing, and it is primary every 12h flushing seed,
It is ready for sowing after seed shows money or valuables one carries unintentionally；
It sows in the greenhouse: taking diameter 10cm, the flowerpot of depth 10cm fills 2/3 Nutrition Soil in flowerpot, and surface is poured
Water is rushed to the wheat seed to have sprouted with sprouting after water fully penetrates into soil, and root mode directed downwardly is uniformly seeded in
In flowerpot, flowerpot surface is covered, then sprinkles one layer of Nutrition Soil in the surface of the seed, thickness is advisable with covering seed, compacting；To small
Wheat is long to 1 heart stage of 2 leaf, when highly reaching 6cm, as small brown rice planthopper feeding feed；
(2), the raising of small brown rice planthopper:
The wheat in flowerpot is clasped with feeding worm cover, by small brown rice planthopper raising in supporting on the wheat seedling that worm cover clasps, in feeding worm
It is plugged label on the outside of cover, feeding time is marked, every 6-7 days replacement feed 1 time；
Feeding worm cover herein is divided into 2 different sizes, and raise the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is for raising
Support 1-3 age small brown rice planthopper；40 mesh gauzes are for raising the 4 ages above small brown rice planthopper；
Insectary environmental condition are as follows: 23 ± 2 DEG C of temperature, relative humidity 65%~75%, 14h: D dark 10h of illumination.
Wherein, it replaces feed method: opening and support worm cover bottom side, the feeding worm cover bottom let drive at out with inflation pumping hole is blown
Small brown rice planthopper is blown to feeding worm cover top by gas, after blowing clean, is made to support worm cover top down, is avoided small brown rice planthopper from jumping out, then rest worm
Cover is covered in together with small brown rice planthopper on new wheatfeed, and label label in outside changes the seedling date, and seedling process is changed in completion.
It is no on used wheatfeed inspection plant to have ovum, if used feed pulled out without ovum, soil
It pours out, is recycled after sieving；If there is ovum on plant, the consistent wheat seed of plumpness is sprinkled in the gap of wheat seeding, earthing,
Watering, buckles well feeding worm cover, record date, this process is known as sowing relay wheat；Small brown rice planthopper hatches after 10-13 days, relay wheat
The feed for becoming new hatching nymph to 2 leaf, 1 heart is grown.
Small brown rice planthopper large-scale breeding method provided by the invention, it is characterized in that:
1, Nutrition Soil is prepared, need field normal soil: fertilizers for potted flowers Nutrition Soil: vermiculite 2:1:1 makes Nutrition Soil moisture retention more
Good, nutrition is richer.
2, the soak for wheat vernalization are as follows: cycocel: the soak that clear water has been converted according to the ratio of 1:25 makes wheat
Growing way is sturdy after budding, small brown rice planthopper feeding preferably.
3, when replacing small brown rice planthopper feed, small brown rice planthopper is blown to the top of feeding worm cover using the gas of inflator pump blowout, is reduced
The difficulty of manpower purging, saves the time compared with using writing brush to shift small brown rice planthopper, considerably increases transfer efficiency.Small brown rice planthopper
Large-scale breeding needs replacing more feed daily, using inflator pump can make entire breeding process save it is a large amount of artificial and
Time.
4, when replacing feed to the small brown rice planthopper to have laid eggs, relay wheat has been sowed, ensure that the raising in the small brown rice planthopper time of infertility,
And since small brown rice planthopper likes the tender plant of feeding, in small brown rice planthopper breeding process, whole process use children's tender wheat seedling as feed,
Small brown rice planthopper spawning rate is increased, and improves hatching rate and survival rate.
5, small brown rice planthopper raising whole process carries out in the insectary of 15 ㎡, and heat insulation effect is good, the air conditioner of built-in 35W, entirely
Year, temperature control ability was good, and feeding worm frame is all made of stainless steel branch, and support effect is obvious.Cantilever tip be mounted with plant growth lamp and
Timer ensure that wheat and small brown rice planthopper grow required illumination condition.
6, in order to obtain the consistent small brown rice planthopper of development duration, since small brown rice planthopper oviposition, every 1 feed of replacement in 3 days, together
When sow relay wheat, do not mixed with the larva of other period spawning and hatchings with the small brown rice planthopper larva that a batch hatches when changing seedling.
Guarantee that the small brown rice planthopper age in the same feeding worm cover is consistent in this way.
Specific embodiment
It is described in conjunction with the embodiments a specific embodiment of the invention.
1, the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: soil sieving is fetched to field trial, the bulky grains such as stone is weeded out, is then bought back with market
Flower soil, vermiculite prepare Nutrition Soil with the ratio of 2:1:1, spare.
Wheat seed screening, vernalization: selection blade is generous, and plant type is compact, and the wheat breed of growing way stalwartness is as feed.Really
After determining wheat breed, the consistent seed of plumpness is chosen, is soaked in cycocel: the soak that clear water has been converted with the ratio of 1:25
In, it is clean with clear water repeated flushing after impregnating for 24 hours, moisture is drained, the gauze that surface covering has been soaked carries out moisturizing, every 12h
It is primary to rinse seed, is ready for sowing after seed shows money or valuables one carries unintentionally.
Sowing: taking diameter 10cm, and the flowerpot of depth 10cm fills 2/3 Nutrition Soil, surface watering, to water in flowerpot
After fully penetrating into soil, the wheat seed to have sprouted is rushed to sprouting, root mode directed downwardly is uniformly seeded in flowerpot,
It covers flowerpot surface (about 250), then sprinkles one layer of Nutrition Soil in the surface of the seed, thickness is advisable with covering seed, compacting.To
When wheat length to 1 heart stage of 2 leaf, highly about 6cm, small brown rice planthopper feeding feed can be used as.
Above procedure carries out in the greenhouse.
2, the raising of small brown rice planthopper:
The wheat in flowerpot is clasped with the feeding worm cover that diameter 9cm white clear acrylic pipe+gauze is made into, by small brown rice planthopper
It raises in supporting on the wheat seedling that worm cover clasps, supports worm cover upper end and clasped with gauze, rubber band is tied tight.It is plugged on the outside of feeding worm cover
Label marks feeding time.Every 6-7 days replacement feed 1 time.
Feeding worm cover herein is divided into 2 different sizes, and raise the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is for raising
Support 1-3 age small brown rice planthopper；40 mesh gauzes are for raising the 4 ages above small brown rice planthopper.
Small brown rice planthopper replaces feed method: careful open supports worm cover bottom side, opens inflator pump, and inflation pumping hole is let drive at
Small brown rice planthopper is blown to feeding worm cover top by the feeding worm cover bottom blowing opened, and after blowing clean, makes to support worm cover top down, ash is avoided to fly
Lice jumps out, and then rests worm cover and is covered on new wheatfeed together with small brown rice planthopper, and label label in outside changes the seedling date, and seedling is changed in completion
Process.It is no on used wheatfeed inspection plant to have ovum, if used feed pulled out, soil falls without ovum
Out, it is recycled after sieving.If there is ovum on plant, used feed is not abandoned, and sprinkles plumpness one in the gap of wheat seeding
Feeding worm cover, record date are buckled well in the wheat seed of cause, earthing, watering, this process is known as sowing relay wheat.After 10-13 days
Small brown rice planthopper hatching, relay wheat have grown the feed for becoming new hatching nymph to 2 leaf, 1 heart.
Small brown rice planthopper raise in the insectary of 15 ㎡, insectary environmental condition are as follows: 23 ± 2 DEG C of temperature, relative humidity (70 ±
5) %, illumination 14h: dark 10h.
Small brown rice planthopper large-scale breeding method provided by the invention is to corn rough dwarf, wheat rosette stunt, Wheat Yellow item
On the basis of the arbovirus such as line dwarf wilt disease is studied for many years, a set of using wheat as feed, progress small brown rice planthopper has been gradually formed
The method of anniversary raising.This method is easy to operate, and worm age is consistent, and small brown rice planthopper breeding potential is high, realizes the whole year of a large amount of small brown rice planthoppers
Continuous raising can provide enough tests at any time and use worm, meet the experiment needs of more units.
Claims (3)
1. small brown rice planthopper large-scale breeding method, which is characterized in that including following procedure:
(1), the preparation of small brown rice planthopper feeding materials:
The preparation of Nutrition Soil: soil sieving is fetched to field trial, then with commercially available flower soil, vermiculite with volume ratio 2:1:1's
Ratio prepares Nutrition Soil, spare；
Wheat seed screening, vernalization: selection blade is generous, and plant type is compact, and the wheat breed of growing way stalwartness is as feed；It chooses full
The consistent seed of full scale, is soaked in cycocel: in the soak that clear water has been converted with the ratio of volume 1:25, after impregnating for 24 hours, with clear
Water repeated flushing is clean, drains moisture, and the gauze that surface covering has been soaked carries out moisturizing, primary every 12h flushing seed, wait plant
Son is ready for sowing after showing money or valuables one carries unintentionally；
It sows in the greenhouse: taking diameter 10cm, the flowerpot of depth 10cm fills 2/3 Nutrition Soil in flowerpot, and surface is watered,
After water fully penetrates into soil, the wheat seed to have sprouted is rushed to sprouting, root mode directed downwardly is uniformly seeded in flowerpot
In, flowerpot surface is covered, then sprinkles one layer of Nutrition Soil in the surface of the seed, thickness is advisable with covering seed, compacting；It is long to wheat
To 1 heart stage of 2 leaf, when highly reaching 6cm, as small brown rice planthopper feeding feed；
(2), the raising of small brown rice planthopper:
The wheat in flowerpot is clasped with feeding worm cover, by small brown rice planthopper raising in supporting on the wheat seedling that worm cover clasps, is supporting worm cover
Outside is plugged label, feeding time is marked, every 6-7 days replacement feed 1 time；
Feeding worm cover herein is divided into 2 different sizes, and raise the small brown rice planthopper of different larval instar respectively: the gauze of 60 mesh is for raising 1-
3 age small brown rice planthoppers；40 mesh gauzes are for raising the 4 ages above small brown rice planthopper；
Insectary environmental condition are as follows: 23 ± 2 DEG C of temperature, relative humidity 65%~75%, illumination 14h: dark 10h.
2. small brown rice planthopper large-scale breeding method according to claim 1, which is characterized in that replacement feed method: open and support
Small brown rice planthopper is blown to feeding worm cover top, blown clean by worm cover bottom side, the feeding worm cover bottom blowing let drive at out with inflation pumping hole
Afterwards, make to support worm cover top down, avoid small brown rice planthopper from jumping out, then rest worm cover and covered in together with small brown rice planthopper on new wheatfeed,
Label label in outside changes the seedling date, and seedling process is changed in completion.
3. small brown rice planthopper large-scale breeding method according to claim 1, which is characterized in that used wheatfeed inspection
Look into it is no on plant have ovum, if pulled out used feed without ovum, soil is poured out, and is recycled after sieving；If on plant
There is ovum, sprinkles the consistent wheat seed of plumpness in the gap of wheat seeding, feeding worm cover is buckled well in earthing, watering, record date, this
One process is known as sowing relay wheat；Small brown rice planthopper hatches after 10-13 days, and relay wheat has grown to 2 leaf, 1 heart, becomes new hatching nymph
Feed.
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| US10638788B2 (en) | 2016-05-04 | 2020-05-05 | Aspire Food Group USA Inc. | Method and system for spray drying insects |
| US11395474B2 (en) | 2016-05-10 | 2022-07-26 | Aspire Food Group Ltd | Harvesting and incubating systems for cultivation of insects |
| USD841898S1 (en) | 2017-04-13 | 2019-02-26 | Aspire Food Group USA Inc. | Insect habitat stacker |
| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
| CN114831084B (en) * | 2022-06-13 | 2023-05-02 | 湖南省植物保护研究所 | Method for breeding rice planthoppers by using ratoons |
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| CN101715754A (en) * | 2009-12-10 | 2010-06-02 | 浙江省农业科学院 | Method for raising small brown rice planthopper by utilizing soil-less cultured barley seedling |
| CN104012476B (en) * | 2014-06-25 | 2015-11-11 | 江苏省农业科学院 | A kind of method of indoor mass rearing small brown rice planthopper |
| CN105594431B (en) * | 2015-12-24 | 2018-06-26 | 长武县农业技术推广中心 | A kind of arplain winter wheat is naturally plus for mating system |
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