CN114831084B - Method for breeding rice planthoppers by using ratoons - Google Patents
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 159
- 235000009566 rice Nutrition 0.000 title claims abstract description 153
- 241001466042 Fulgoromorpha Species 0.000 title claims abstract description 50
- 238000009395 breeding Methods 0.000 title claims abstract description 23
- 230000001488 breeding effect Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 21
- 240000007594 Oryza sativa Species 0.000 title description 7
- 241000209094 Oryza Species 0.000 claims abstract description 153
- 230000001172 regenerating effect Effects 0.000 claims abstract description 18
- 238000003306 harvesting Methods 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 19
- 239000004202 carbamide Substances 0.000 claims description 19
- 239000003337 fertilizer Substances 0.000 claims description 15
- 241000238631 Hexapoda Species 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 229940072033 potash Drugs 0.000 claims description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 3
- 235000015320 potassium carbonate Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000009340 sequential cropping Methods 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims 1
- 239000010902 straw Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 241001556089 Nilaparvata lugens Species 0.000 description 8
- 241000819999 Nymphes Species 0.000 description 6
- 241000176086 Sogatella furcifera Species 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 241001498622 Cixius wagneri Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241001674048 Phthiraptera Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000004227 basal ganglia Anatomy 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
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- -1 s Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a method for breeding rice planthoppers by using ratoons, which is characterized in that new rice seedlings can be regenerated under proper conditions for 7-10 days after the ratoons are harvested in the first season, and feeding and spawning sites are provided for the rice planthoppers. The invention utilizes the advantages of obvious time saving, labor saving and cost saving of breeding the rice planthoppers by the regenerative rice, and is beneficial to the indoor large-scale breeding of the rice planthoppers.
Description
Technical Field
The invention belongs to the field of artificial breeding of insects, and particularly relates to a method for breeding rice planthoppers by using ratoons.
Background
Rice is the most important grain crop in China, occupies very important position in the national grain production, and the planting area and the yield of the rice are in the forefront of the world. Rice planthoppers (mainly brown planthoppers Nilaparvatalouges)
Bai Beifei Trinitella furcifera (Horv th) and Laodelphax striatellus (Fall en)) are one of the most main rice pests in main rice planting areas in China and even southeast Asia, not only seriously affect the rice yield, but also can spread various rice virus diseases, and in view of hazard and occurrence areas, the rice planthoppers are always a hot spot research object in the aspect of rice plant protection science professions.
In order to study biological, physiological and molecular mechanisms of rice planthoppers, a laboratory related to rice planthoppers needs to carry out large-scale artificial feeding under the condition of the laboratory. In most laboratories, in order to obtain biological characteristics of rice planthoppers with consistent biological characteristics, such as consistent resistance level, consistent age, consistent seed source property and the like, the rice planthoppers must be subjected to long-period subculture.
Rice is the main host plant for breeding rice planthoppers. Laboratory to obtain rice planthoppers required for the test, a large number of rice seedlings need to be cultivated. The study shows that each physiological parameter (such as life, spawning quantity, honeydew quantity and the like) of the rice planthoppers is better than the early growth period (such as seedling stage and tillering stage) of the rice in the booting stage and later development stage of the rice. However, rice needs a long time to develop to booting stage, and better rice Miao Jizhu can be obtained only under proper indoor growth conditions (especially in winter), and the stress of the environmental factors on the growth of rice seedlings is particularly obvious in winter, so that the test expenditure budget is increased. Most laboratories adopt seedling stage rice seedlings to feed rice planthoppers, and as the seedling stage rice Miao Naidao planthoppers have weak invasive capability, hosts need to be frequently replaced, the unfavorable Yu Daofei lice are accurately and stably fed, and parameters such as growth and development of the rice planthoppers on the seedling stage rice seedlings are poorer than those of the later stage of the rice seedlings (such as booting stage and later growth stage). Meanwhile, in order to obtain clean host rice seedlings, independent isolation spaces are needed for the rice seedling culture room and the rice planthopper breeding room. Thus, the laboratory is time, space, labor and expense consuming to obtain a suitable seedling host.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: how to provide a method for raising rice planthoppers in an indoor scale, so as to save time, labor and cost.
The technical scheme of the invention is as follows: a method for breeding rice planthoppers by using ratoons, comprising the following steps:
step one: the measures before harvesting the first season rice of the ratoon rice: applying urea 7 days before the first season, and irrigating 1-3cm one day after applying urea;
step two: harvesting the rice in the first season: a high stubble-leaving harvesting method is adopted, and the stubble-leaving height is 50-60cm;
step three: the water and fertilizer management measures after harvesting the rice are as follows: the field is covered with water after the reaping of the ratooning rice, and the height of a water layer is 3-5cm; applying additional fertilizer urea and potash fertilizer 3-7 days after harvesting the first-season rice;
step four: the rice is moved indoors and daily management:
1) Preparation: after harvesting, the new-grown rice seedlings with the height of 5-10cm are moved into the indoor feeding rice planthoppers;
2) Transplanting: digging the regenerative rice stump with mud, transplanting the regenerative rice stump with mud into a culture container, and placing the regenerative rice stump with mud into a rearing cage with the temperature of 25+/-2 ℃ and the humidity of 75+/-5% and the photoperiod light-dark ratio of 14h to 10 h;
3) And (3) daily management of transplanting: the height of the nutrient solution water layer in the culture container is ensured to be 1-3cm in daily life;
step five: and (3) insect inoculation: inoculating 3-5 female rice planthoppers and 1-3 male rice planthoppers into each pot of regenerated rice seedlings of the initially-emerged rice planthoppers; or inoculating 2-or 3-year nymphs of rice planthoppers with consistent ages at 10-15 heads/basin;
step six: feeding:
1) And if the fifth step is to connect adults: after the new hatched nymphs of the next generation appear, sucking the nymphs into clean regenerated rice seedlings, wherein the grafted regenerated rice seedlings can maintain the life span of F0-generation adults and the hatching period of F1-generation adults;
2) If step six, nymphs are grafted: and F0 generation nymphs are maintained to develop to an adult stage by the young rice seedlings, and after the adults appear, newly emerged adults are matched and inoculated onto clean young rice seedlings according to the fifth step.
Further, in the first step, urea is applied at a speed of 5-7.5 kg/667 square meter.
Further, in the third step, urea is 5-10 kg/667 square meters, and potassium chloride is 5-7.5 kg/667 square meters.
Further, in step four, 1), before transplanting, the head Ji Daodao stubble of leaf sheaths withered on the outer layer are peeled off until the vital rice stems are exposed, and meanwhile, the new-born and regenerated rice seedlings are checked to ensure no insect damage.
Further, in step four, 3), the nutrient solution comprises the following components in proportion: NH (NH) 4 NO 3 8.0g、KH 2 PO 4 2.5g、K 2 SO 4 3.4g、CaCl 2 3.3g and MgCl 2 1.2g, 100L of water.
The regenerated rice is characterized in that after harvesting the rice in the first season (multi-finger middle rice), the side buds on the basal ganglia are germinated again, tillered and heading again by utilizing the tillering regeneration capability of rice piles and combining proper fertilization, irrigation and comprehensive prevention and control of disease and insect pests, so that the purpose of harvesting the rice in one season is achieved.
The rice variety of the invention is selected from the rice with the characteristics of proper growth period, lodging resistance, strong regeneration capacity, proper plant height, susceptibility to rice planthoppers and the like.
The regenerative rice utilizes the nutrient, photosynthetic capacity and root system absorption capacity of the head Ji Dao, the seedling emergence is rapid, and 1-3 regenerative seedlings can be generated by one seedling of the head rice in the season, so that more feeding positions, activities and space for avoiding natural enemies can be provided for rice planthoppers. The recycling rice can save a large amount of seeds, water and chemical fertilizer in the aspect of water and fertilizer management can be saved, and meanwhile, the labor cost of a laboratory is saved. After harvesting the first-season rice, the new rice seedlings can be regenerated under proper conditions for 7-10 days, and feeding and spawning sites are provided for rice planthoppers. The breeding of rice planthoppers by using the ratoons has the advantages of obvious time saving, labor saving and cost saving.
Compared with the prior art, the invention has the following beneficial effects:
the invention utilizes the advantages of obvious time saving, labor saving and cost saving of breeding the rice planthoppers by the regenerative rice, and is beneficial to the indoor large-scale breeding of the rice planthoppers.
Detailed Description
The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, were purchased from commercial sources.
Step one: the measures before harvesting the first season rice of the ratoon rice: urea is applied about 7 days before the first season is mature, and water is irrigated for 1-3cm about one day after the urea is applied. The germination-protecting and germination-accelerating fertilizer is prepared by applying 5-7.5 kg/667 square meter of urea about 7 days before the first season is mature, so that the early germination of the regenerated buds can be promoted and the number of seedlings can be obviously increased. Varieties of ratooning rice: the bred regenerated rice variety has the characteristics of proper growth period, lodging resistance, strong regeneration capacity, proper plant height, susceptibility to rice planthoppers and the like.
Step two: harvesting the rice in the first season: a high stubble-leaving harvesting method is adopted, and the stubble-leaving height is about 50 cm to 60cm; the small-area ratoon rice can be harvested by adopting a manual harvesting method, and the large-area ratoon rice can be harvested by adopting a harvester with a narrow-track-width harvesting platform.
Step three: the water and fertilizer management measures after harvesting the rice are as follows: the rice can be covered with water after harvesting, and the height of the water layer is preferably 3-5cm; the additional fertilizer urea and potash fertilizer are applied 3-7 days after the harvesting of the first-season rice of the ratoon rice, the urea is 5-10 kg/667 square meters, and the potassium chloride is 5-7.5 kg/667 square meters.
Step four: the ratoons were transferred into the laboratory and daily management:
1) Preparation: after the rice seedlings are harvested for 10 days, the new rice seedlings with the heights of 5cm to 10cm can be transferred into a laboratory to raise rice planthoppers. Before transplanting, the head Ji Daodao stubble of leaf sheaths withered on the outer layer are peeled off until the vital rice stems are exposed. Meanwhile, checking whether the newly-grown regenerated rice seedlings have spawning marks of rice planthoppers, and eliminating other rice pests, natural enemies or other insects on rice stubble.
2) Transplanting: digging 15cm multiplied by 15cm square by utilizing a spade to carry out mud connection on the regenerative rice stump, transplanting the regenerative rice stump into a plastic basin with proper size, and placing the plastic basin into a laboratory raising cage with the temperature of 25+/-2 ℃ and the humidity of 75+/-5% and the photoperiod of 14h to 10h (light: dark).
3) And (3) daily management of transplanting: the height of the nutrient solution layer in the plastic basin is ensured to be about 1 cm to 3cm in daily life. The nutrient solution comprises the following components (100L nutrient solution is required by the components and the dosage): NH (NH) 4 NO 3 (8.0g)、KH 2 PO 4 (2.5g)、K 2 SO 4 (3.4g)、CaCl 2 (3.3 g) and MgCl 2 (1.2g)。
Step five: and (3) insect inoculation: the method comprises the steps of (1) inoculating 3-5 female rice planthoppers and 1-3 male rice planthoppers to each pot of regenerated rice seedlings of the initially-emerged rice planthoppers; or the 2-age or 3-age nymphs of rice planthoppers with the same age can be accessed by 10-15 heads/basin.
Step six: feeding:
1) And (3) adult inoculation: after the new hatched nymphs of the next generation appear, the nymphs are sucked and transferred onto clean regenerated rice seedlings, and the grafted regenerated rice seedlings can maintain F 0 Life span and F of adult 1 A hatching period;
2) And (3) inoculating nymphs: the seedling of the regenerated rice can maintain F according to the proportion of the above-mentioned insects 0 The nymphs develop to the adult stage, and after the adults appear, the newly emerged adults are matched and inoculated onto clean rice seedlings.
Application example 1: brown planthopper laboratory subculture
Selecting Q two-priority 851, and performing normal water and fertilizer and chemical control management on the first-season rice; applying urea 5.0 kg/667 square meter 7d (8 months and 8 days) before harvesting, and 15 days after 8 monthsHarvesting, and covering water after harvesting, wherein the depth of the water is 5cm; fertilizing 7.5 kg/667 square meter of urea and 5 kg/667 square meter of potassium chloride 3d (18 days 8 months) after harvesting, and keeping the shallow water layer in the field at 5cm; 10d (8 months and 25 days) after harvesting, transferring into laboratory with the method, and respectively inoculating brown planthoppers (F) in two batches at 8 months and 27 days 0 Generation) adults and nymphs, and performing a subculture test respectively.
The control treatment is TN1, which is a common host variety in a laboratory, and the control treatment is carried out in the seedling growth period common in the laboratory when the insects are inoculated, wherein about 110 rice seedlings are grown in each pot.
Laboratory conditions during feeding: temperature 25+ -2deg.C, humidity 75+ -5%, photoperiod 14h:10h (light: dark) condition.
TABLE 1 influence of Oryza sativa and TN1 seedlings on the subculture of brown planthoppers (F 0 Is instead of adults
Note that: f (F) 1 And F 2 Data are mean values of three replicates; f (F) 2 For secondary breeding, the grafted adults are F 1 Emerging adults are replaced by the newly emerged adults; f (F) 1 And F 2 The nymph is bred by uniformly dividing 3 pots in the treatment of the regenerative rice and by uniformly dividing 4 pots in the treatment of TN1 seedlings.
TABLE 2 Effect of Oryza sativa and TN1 seedlings on the subculture of brown planthoppers (F0 generation is nymph)
Note that: f1 and F2 data are the average of three replicates; f2 is subculture, and the grafted nymphs are F1 generation 3-age nymphs; f (F) 1 And F 2 The nymph is bred by uniformly dividing 3 pots in the treatment of the regenerative rice and by uniformly dividing 4 pots in the treatment of TN1 seedlings.
Conclusion: compared with the conventional method for breeding brown planthoppers by using TN1 seedlings, the method for breeding brown planthoppers by using the secondary breeding of the rice can obtain more brown planthoppers, and has the advantages of less time consumption, low seedling replacement frequency and the like.
Application example 2: subculture of sogatella furcifera in laboratory
Selecting long two-excellent 248, and performing normal water and fertilizer and chemical control management on first-season rice; applying fertilizer to urea 5.0 kg/667 square meter 7d (8 months and 9 days) before harvesting, harvesting for 8 months and 16 days, and covering water after harvesting, wherein the depth of the water is 5cm; fertilizing 7.5 kg/667 square meter of urea and 5 kg/667 square meter of potassium chloride 3d (19 days of 8 months) after harvesting, and keeping a shallow water layer in the field at 5cm; 10d (8 months and 26 days) after harvesting, transferring into a laboratory by the method, and respectively inoculating the plant hoppers (F) into two batches of plant hoppers in 8 months and 28 days 0 Generation) adults and nymphs, respectively, continue the subculture experiments.
The control treatment is TN1, which is a common host variety in a laboratory, and the control treatment is carried out in the seedling growth period common in the laboratory when the insects are inoculated, wherein about 110 rice seedlings are grown in each pot.
Laboratory conditions during feeding: temperature 25+ -2deg.C, humidity 75+ -5%, photoperiod 14h:10h (light: dark) condition.
TABLE 3 influence of secondary feeding of Oryza sativa and TN1 Oryza sativa Miao Duibai on the off-plant hoppers (adult F0 generation)
Note that: f1 and F2 data are the average of three replicates; f2 is subculture, and the grafted adults are F1 generation newly emerged adults; the F1 and F2 nymphs are bred in 3 pots in the regenerative rice treatment and in 4 pots in the TN1 seedling treatment.
TABLE 4 influence of secondary feeding of Oryza sativa and TN1 Oryza sativa Miao Duibai on the number of off-plant hoppers (F0 generation is nymph)
Note that: f1 and F2 data are the average of three replicates; f (F) 2 For secondary breeding, the nymph is F 1 3 rd generation nymphs; f (F) 1 And F 2 The nymph is bred by uniformly dividing 3 pots in the treatment of the regenerative rice and by uniformly dividing 4 pots in the treatment of TN1 seedlings.
Conclusion: compared with the conventional method for breeding the sogatella furcifera by using TN1 seedlings, the method for breeding the sogatella furcifera by using the regenerative rice in a subculture way can obtain more sogatella furcifera and has the advantages of less time consumption, low seedling replacement frequency and the like.
Therefore, compared with the conventional method for breeding rice planthoppers by using TN1 seedlings, the method for breeding rice planthoppers by using the regenerative rice subculture of the invention can obtain more rice planthoppers, and has the advantages of less time consumption, low seedling replacement frequency and the like.
Claims (5)
1. A method for breeding rice planthoppers by using ratoons, which is characterized by comprising the following steps:
step one: the measures before harvesting the first season rice of the ratoon rice: applying urea 7 days before the first season, and irrigating 1-3cm one day after applying urea;
step two: harvesting the rice in the first season: a high stubble-leaving harvesting method is adopted, and the stubble-leaving height is 50-60cm;
step three: the water and fertilizer management measures after harvesting the rice are as follows: the field is covered with water after the reaping of the ratooning rice, and the height of a water layer is 3-5cm; applying additional fertilizer urea and potash fertilizer 3-7 days after harvesting the first-season rice;
step four: the rice is moved indoors and daily management:
1) Preparation: after harvesting, the new-grown rice seedlings with the height of 5-10cm are moved into the indoor feeding rice planthoppers;
2) Transplanting: digging the regenerative rice stump with mud, transplanting the regenerative rice stump with mud into a culture container, and placing the regenerative rice stump with mud into a rearing cage with the temperature of 25+/-2 ℃ and the humidity of 75+/-5% and the photoperiod light-dark ratio of 14h to 10 h;
3) And (3) daily management of transplanting: the height of the nutrient solution water layer in the culture container is ensured to be 1-3cm in daily life;
step five: and (3) insect inoculation: inoculating 3-5 female rice planthoppers and 1-3 male rice planthoppers into each pot of regenerated rice seedlings of the initially-emerged rice planthoppers; or inoculating 2-or 3-year nymphs of rice planthoppers with consistent ages at 10-15 heads/basin;
step six: feeding:
1) And if the fifth step is to connect adults: after the new hatched nymphs of the next generation appear, sucking the nymphs into clean regenerated rice seedlings, wherein the grafted regenerated rice seedlings can maintain the life span of F0-generation adults and the hatching period of F1-generation adults;
2) If the fifth step is to connect nymphs: and F0 generation nymphs are maintained to develop to an adult stage by the young rice seedlings, and after the adults appear, newly emerged adults are matched and inoculated onto clean young rice seedlings according to the fifth step.
2. The method according to claim 1, characterized in that in step one urea is applied in an amount of 5-7.5 kg/667 square meter.
3. The method according to claim 1, wherein in step three urea is 5-10 kg/667 square meter and potassium chloride is 5-7.5 kg/667 square meter.
4. The method of claim 1, wherein in step four, 1), the sheath withered on the outer layer of the head Ji Daodao stubble is peeled off until the viable rice straw is exposed, and the inspection is performed to ensure that the newly regenerated rice seedlings are free from insect pests.
5. The method according to claim 1, wherein in step 3) of the fourth step, the nutrient solution comprises the following components: NH (NH) 4 NO 3 8.0g、KH 2 PO 4 2.5g、K 2 SO 4 3.4g、CaCl 2 3.3g and MgCl 2 1.2g, 100L of water.
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| CN106305632B (en) * | 2016-08-29 | 2019-01-29 | 河北省农林科学院植物保护研究所 | Small brown rice planthopper large-scale breeding method |
| CN106614403A (en) * | 2016-12-30 | 2017-05-10 | 福建省农业科学院水稻研究所 | Rice planthopper breeding and observing apparatus and use method of same |
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| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
| CN108371149A (en) * | 2018-04-27 | 2018-08-07 | 福建省农业科学院植物保护研究所 | A kind of device and its application method of interior mass rearing planthopper |
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