CN104012476B - A kind of method of indoor mass rearing small brown rice planthopper - Google Patents
A kind of method of indoor mass rearing small brown rice planthopper Download PDFInfo
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- CN104012476B CN104012476B CN201410298198.6A CN201410298198A CN104012476B CN 104012476 B CN104012476 B CN 104012476B CN 201410298198 A CN201410298198 A CN 201410298198A CN 104012476 B CN104012476 B CN 104012476B
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Abstract
The invention provides a kind of method of indoor mass rearing small brown rice planthopper, belong to technical field of insect feeding.It comprises the cultivation of rice seedlings, three batches of time small brown rice planthoppers not of the same age raising, change the steps such as seedling.The present invention adopts glass beaker refinement earth culture to educate rice seedlings, cultivates and does not limit by natural conditions such as place, time, environment; Three batches of time small brown rice planthoppers not of the same age are raised simultaneously, experiment can be provided at any time with time standard examination worm in age, strictly control the stocking density of three batches of worms, ensure that the acquisition with small brown rice planthopper desired nutritional that makes full use of of rice seedlings, not only reduce and change seedling number of times, and substantially increase the fertility of small brown rice planthopper; Change seedling independently changing between seedling and carry out, use the black cloth that small brown rice planthopper can be made to drive light to block, can effectively avoid mixing of small brown rice planthopper different population, ensure that the homogeneity of small brown rice planthopper population.The present invention has easy and simple to handle, flexible, and worm is neat, abundant for age, and breeding amount is large, raises the advantages such as stable, can provide sufficient standard examination worm for test.
Description
Technical field
The present invention relates to a kind of indoor mass rearing small brown rice planthopper method and in application agriculturally, belong to technical field of insect feeding.
Background technology
Small brown rice planthopper (Laodelphaxstriatellus
) belong to Homoptera (Homoptera) Delphacidae, be distributed widely in the ground such as East Asia, Southeast Asia, Europe and north African, domestic distribution is throughout various places, can inhale and take food the multiple gramineous crops such as paddy rice, corn, wheat, barley by thorn, various plants virus can also be propagated, its biography poison endangers the loss caused and is far longer than directly thorn suction harm, is one of insect that current agricultural production is widely known by the people.In recent years, due to the impact of the factors such as the change of cropping system, the abuse of pesticide control and Climate Anomalies, the population density of small brown rice planthopper in various places is in continuing ascendant trend, so small brown rice planthopper has developed into the primary pest of Asia paddy rice since the 1950's, its virus disease propagated also has become disease important on paddy rice.There are some researches show that the stripe disease of Jiangsu generation in 2004 reaches 2,357 ten thousand mu, account for 79% of the Jiangsu Rice Cropping gross area, the occurring area at present in the whole nation is more than 5,000 ten thousand mu, and No kernels or seeds are gathered, as in a year of scarcity in grave illness field.And its propagate black streaked dwarf virus of rice at present in Anhui, Jiangxi and Fujian the north also have distribution in the interior central and east, the Yangtze river basin, be outbreak of epidemic situation in various places, Jiangsu since 2007, be a destructive disease to whole rice workspace, cause huge economic loss.
Small brown rice planthopper becomes huge obstacle agricultural production being captured high yield and bumper harvest gradually, is the primary study object of the plant protection departments of science and technology.Will to the harmful characteristics of small brown rice planthopper itself, the mutual work passing malicious characteristic and host and virus and pesticide resistance, the pathogenesis of virus disease and pathogenic characteristic, the work of qualification and screening etc. the aspect of resistant material is furtherd investigate, and indoor mass rearing small brown rice planthopper is the basis of research.Therefore, a large amount of small brown rice planthopper standard examination worms must be provided the anniversary, needed for guarantee test.
Document announcement has the method for following several frequently seen indoor feeding plant hopper: 1, a kind of rice fulgorid indoor rice bud method for breeding.Describe and a kind ofly apply the method that paddy rice rice bud and special foster worm device raise rice fulgorid, the method is because raising a large amount of small brown rice planthopper with rice bud soilless-cultivated seedling, and seedling raise period is short and insect density large, thus substantially reduces and change the seedling cycle, and worm is also unanimously more difficult for age in addition.2, the method for north of china in winter indoor feeding small brown rice planthopper.Describe equally application rice seedling and tillering stage wheat as host plant for rearing, turn worm case and dependent insect cage is raised with special, although this method shortens change the seedling cycle, alms bowl dish etc., utensil takes up room too large, operates still not too easy.3, the indoor amount reproduction of small brown rice planthopper and winter feeding method.Spring, the summer, autumn rice seedlings and raise box and raise, winter wheat and the native vinyl disc of band raise, method of operating is comparatively complicated.4, a kind of short-cut method of indoor feeding small brown rice planthopper.Be that medium plastic casing cultivation rice seedlings is raised with sponge, single seedling single head method acquisition small brown rice planthopper in the same length of time, the worm amount that can provide is limited.5, small brown-back rice plant-hopper raising and raise poison.Mainly describe 1-3 for small brown rice planthopper raising with raise malicious method, complex operation and many generations can not be carried out raise, breeding cycle is long and cannot provide a large amount of standard worm source.6, Preliminary report of healthy rearing plants for Laodelphax striatellus Fallen in indoor artificial feeding.The wheat of apparatus cane and barnyard grass raise adult, raise nymph with wheat and barnyard grass seedling, and breeding cycle is grown and is subject to the restriction such as place and temperature.As can be seen from the rearing method of above several small brown rice planthopper, suitable host plant for rearing, instrument and place is selected to be the keys of carrying out small brown rice planthopper artificial feeding.This research department to small brown rice planthopper and through its propagate two-strain (rice stripe virus, Ricestripevirus, RSV) and (rice black-streaked dwarf virus, riceblack-streakeddwarfvirus, RBSDV) on the basis studied for many years, grope a set of can in the method for indoor mass rearing small brown rice planthopper, the method is easy and simple to handle, the whole year raise, worm is neat for age, continuous raising is stablized, and a large amount of standard can be provided to try worm, greatly meets experiment needs.
Summary of the invention
Raise the deficiency of small brown rice planthopper method based on indoors artificial in existing data, object of the present invention aim to provide a kind of by such environmental effects, easy and simple to handle, breeding cycle is short, the length of time is neat, reproduction rate is high, the annual method of raising continuously, can provide the indoor feeding small brown rice planthopper of required worm age and worm amount at any time.
The method of described indoor mass rearing small brown rice planthopper, its feature comprises following processing step:
1) rice seed soaking and vernalization: get the some grains of full rice paddy seed, shrivelled seed is removed with clear water rinsing, use Seed soaking 48h again, repeatedly rinse with clear water after 48h, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 24-28 DEG C of insectary, in order to moisturizing can cover one piece of wet towel, after 48-60h, namely can be used to sowing.
2) cultivation of rice seedlings: clean in the 1000mL glass beaker dried and add preprepared fine earth (thick about 2cm), add water along walls of beaker.To the seed of bud be urged to be sprinkling upon uniformly on soil, about 100 every glass, the fine earth then spilling thin layer covers seed.Cover beaker mouth with gauze and prick with rubber band tight.Broadcast on foster worm frame that the beaker of planting is placed on insectary and grow, treat that rice seedlings grows to about 6cm and namely can be used for raising small brown rice planthopper.
3) small brown rice planthopper fed-batch: raise small brown rice planthopper in three batches under normal foster worm condition.Get some small brown rice planthopper nymphs for first batch and be placed in step 2) newly educate on rice seedlings, totally 10 glasss; Get some 3-4 small brown rice planthoppers in age for second batch to be placed in and newly to educate on rice seedlings, totally 10 glasss; Getting some striatellus imagos for 3rd batch raises in newly educating on rice seedlings, totally 10 glasss.Every glass all covers gauze rubber band and ties and obturage, and beaker puts the worm date with black marking pen mark, is then positioned over normal management on foster worm frame and raises.A seedling is changed every 8-10 days, when changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivated, interface is muffled with the black cloth of one piece of width 20cm, length 40cm, with writing brush to put in worm beaker gently small brown rice planthopper brush is entered below newly to educate on rice seedling, mark on beaker with black marking pen and put the worm date, just complete with the sealing of gauze rubber band and change seedling process.If the worm below the small brown rice planthopper changed 4 age, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worms more than 4 ages of changing, then old seedling will stay growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, general from changing the little nymph of white can seen after Miao Zhi counts 7 days and rice seedling occur successively hatch, within 10 days, teleonymph is hatched completely substantially, then treats nymph to be changed to according to quantity distribution principle for 2-3 days on seedling and newly to educate on rice seedling normal management and raise.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 1) in visually observe rice seed after vernalization 48h and whether show money or valuables one carries unintentionally, can sow if major part has showed money or valuables one carries unintentionally; Show money or valuables one carries unintentionally if having no to place again after 12h observes and wait to sow.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in sowing time soil in add water moistening, if it is excessive to add water, seed can rot mouldy after sowing, not easily germinate, and with foul smell.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in insectary select heat insulation effect better size to be about 10m
2idle room, require that water power is easy to use, an in-built changes in temperature convertible frequency air-conditioner; Support worm frame to be made up of stainless steel stent, transparent toughened glass, plant growth lamp, timer etc.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) middle culture of pests condition: temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in management of watering after planting: treat after 48h that seed rice germinates about the 1cm that is unearthed, and waters first time water, is as the criterion so that soil is moistening; After every 48h observe water, treat seedling grow to can be used for small brown rice planthopper raise till.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in normal to support worm condition be temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in first batch to raise the density of nymph be 300-400 head/cup; Second batch of density of raising 3-4 instar is 200-300 head/cup; 3rd batch of density of raising adult is 100-200 head/cup, controls the growing way that stocking density effectively can control to raise rice seedling, makes small brown rice planthopper fully absorb nutrition, grow up strong and sturdy.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in change seedling be raise the independent room that small brown rice planthopper separates completely with placing between selection one, be called and change between seedling.Seedling is easy to operate is advisable to change to change size between seedling, and the small brown rice planthopper that can avoid like this escaping out when changing seedling is mixed in insectary to be raised in small brown rice planthopper, effectively ensure raise the homogeneity of small brown rice planthopper population.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in normal management raise in the most important thing is the amount that controls to water, the general 48h of rice seedlings raising small brown rice planthopper waters a water, and the amount of watering is as the criterion with complete wetting native in cup; Before needing to change the cup of seedling, 24h can not water, until rice seedlings and soil all dry after just can change seedling, if both wet words of changing seedling, small brown rice planthopper can be bonded on writing brush dead, the soil at the cup end also can fall down sometimes, not only makes troubles to operation, also can cause the loss of the small brown rice planthopper number of animals raised.
According to said method raise, rice seedlings cultivated by application beaker, and the glass wall light transmission of beaker is better, and rice seedlings can absorb sufficient light source, grows comparatively vigorous, can provide sufficient nutrition for small brown rice planthopper; Also availability that is comparatively easy, quick, rice seedling is also high when changing the operations such as seedling for beaker in addition.Raise small brown rice planthopper in three batches, exist while just in time ensure that three kinds of worm states, the experiment that various worm state of can satisfying the demand at any time is carried out, and foster worm scale can be adjusted at any time according to by worm amount, both provide enough standard examination worms, be unlikely to again the excess waste causing worm amount.The small brown rice planthopper that this method is raised can be applicable to insect biology habit, insect and the mutual work of host, a series of research such as excavation qualification, disease-resistant variety seed selection of variety source.
Accompanying drawing explanation
Fig. 1 is the insectary indoor scene schematic diagram of indoor mass rearing small brown rice planthopper.
Specific implementation method
The present invention is further illustrated below by specific embodiment.
In following examples foodstuff source and infrastructure as follows:
Paddy rice, kind " Wu-Yu-Geng 3 ", Plant Protection Inst., Jiangsu Academy of Agriculture is from numerous rice seed; Insectary: be about 10m depending on scale selection better size of heat insulation effect
2idle room, require that water power is easy to use, an in-built changes in temperature convertible frequency air-conditioner; Support worm frame: 125cm × 40cm × 200cm support worm frame several, each shelf divides 4 layers, the transparent toughened glass that every layer of pad one piece of quality is thicker, and it is the plant growth lamp of 40W that three specifications are installed at every layer of top, light source is downward, and microcomputer time-controlled switch installed by each shelf; Change between seedling: raise the independent room that small brown rice planthopper separates completely between one with placement, to change, seedling is easy to operate is advisable size, the built-in tables and chairs that conveniently can change seedling.
Embodiment 1
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. rice seedlings is cultivated: get full Wu-Yu-Geng 3 rice paddy seed, shrivelled seed is removed with clear water rinsing, use Seed soaking 48h again, repeatedly rinse with clear water after 48h, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 24 DEG C of insectaries, in order to moisturizing can cover one piece of wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and namely can be used to sowing; First add preprepared fine earth (thick about 2cm) cleaning in the beaker dried during sowing, adding water along walls of beaker infiltrates soil.Be sprinkling upon uniformly on soil by the seed rice showed money or valuables one carries unintentionally, about 100 every glass, the fine earth then spilling thin layer covers seed again.Cover beaker mouth with gauze and prick with rubber band tight; Broadcast the beaker of planting to continue to be placed on temperature 24 DEG C, relative moisture 70%, the foster worm frame of light application time 14h, intensity of illumination 5000LUX grows, treat that rice seedlings grows to about 6cm and namely can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: temperature 24 DEG C, relative moisture 70%, raise three batches of small brown rice planthoppers in different worm age under the insectary condition of light application time 14h, intensity of illumination 5000LUX, each batch raises about 10 glasss, and first batch of density of raising nymph is 300/glass; Second batch of density of raising 3-4 instar is 200/glass; 3rd batch of density of raising adult is 100/glass.Every glass all covers gauze rubber band and ties and obturage, and beaker puts the worm date with black marking pen mark, is then positioned over normal management on foster worm frame and raises.
3. seedling is changed: changed a seedling every 8 days changing between seedling, when changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivated, interface is muffled with the black cloth of one piece of width 20cm, length 40cm, with writing brush to put in worm beaker gently small brown rice planthopper brush is entered below newly to educate on rice seedling, mark on beaker with black marking pen and put the worm date, just complete with the sealing of gauze rubber band and change seedling process.If the worm below the small brown rice planthopper changed 4 age, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worms more than 4 ages of changing, then old seedling will stay growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, general from changing the little nymph of white can seen after Miao Zhi counts 7 days and rice seedling occur successively hatch, within 10 days, teleonymph is hatched completely substantially, then treats nymph to be changed to according to quantity distribution principle for 2-3 days on seedling and newly to educate on rice seedling normal management and raise.
Embodiment 2
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. rice seedlings is cultivated: get full Wu-Yu-Geng 3 rice paddy seed, shrivelled seed is removed with clear water rinsing, use Seed soaking 48h again, repeatedly rinse with clear water after 48h, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 26 DEG C of insectaries, in order to moisturizing can cover one piece of wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and namely can be used to sowing; First add preprepared fine earth (thick about 2cm) cleaning in the beaker dried during sowing, adding water along walls of beaker infiltrates soil.Be sprinkling upon uniformly on soil by the seed rice showed money or valuables one carries unintentionally, about 100 every glass, the fine earth then spilling thin layer covers seed again.Cover beaker mouth with gauze and prick with rubber band tight; Broadcast the beaker of planting to continue to be placed on temperature 26 DEG C, relative moisture 80%, the foster worm frame of light application time 14h, intensity of illumination 6000LUX grows, treat that rice seedlings grows to about 6cm and namely can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: temperature 26 DEG C, relative moisture 80%, raise three batches of small brown rice planthoppers in different worm age under the insectary condition of light application time 14h, intensity of illumination 6000LUX, each batch raises about 10 glasss, and first batch of density of raising nymph is 350/glass; Second batch of density of raising 3-4 instar is 250/glass; 3rd batch of density of raising adult is 150/glass.Every glass all covers gauze rubber band and ties and obturage, and beaker puts the worm date with black marking pen mark, is then positioned over normal management on foster worm frame and raises;
3. seedling is changed: changed a seedling every 8 days changing between seedling, when changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivated, interface is muffled with the black cloth of one piece of width 20cm, length 40cm, with writing brush to put in worm beaker gently small brown rice planthopper brush is entered below newly to educate on rice seedling, mark on beaker with black marking pen and put the worm date, just complete with the sealing of gauze rubber band and change seedling process.If the worm below the small brown rice planthopper changed 4 age, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worms more than 4 ages of changing, then old seedling will stay growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, general from changing the little nymph of white can seen after Miao Zhi counts 7 days and rice seedling occur successively hatch, within 10 days, teleonymph is hatched completely substantially, then treats nymph to be changed to according to quantity distribution principle for 2-3 days on seedling and newly to educate on rice seedling normal management and raise.
Embodiment 3
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. rice seedlings is cultivated: get full Wu-Yu-Geng 3 rice paddy seed, shrivelled seed is removed with clear water rinsing, use Seed soaking 48h again, repeatedly rinse with clear water after 48h, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 28 DEG C of insectaries, in order to moisturizing can cover one piece of wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and namely can be used to sowing; First add preprepared fine earth (thick about 2cm) cleaning in the beaker dried during sowing, adding water along walls of beaker infiltrates soil.Be sprinkling upon uniformly on soil by the seed rice showed money or valuables one carries unintentionally, about 100 every glass, the fine earth then spilling thin layer covers seed again.Cover beaker mouth with gauze and prick with rubber band tight; Broadcast the beaker of planting to continue to be placed on temperature 28 DEG C, relative moisture 90%, the foster worm frame of light application time 14h, intensity of illumination 7000LUX grows, treat that rice seedlings grows to about 6cm and namely can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: temperature 28 DEG C, relative moisture 90%, raise three batches of small brown rice planthoppers in different worm age under the insectary condition of light application time 14h, intensity of illumination 7000LUX, each batch raises about 10 glasss, and first batch of density of raising nymph is 400/glass; Second batch of density of raising 3-4 instar is 300/glass; 3rd batch of density of raising adult is 200/glass.Every glass all covers gauze rubber band and ties and obturage, and beaker puts the worm date with black marking pen mark, is then positioned over normal management on foster worm frame and raises.
3. seedling is changed: changed a seedling every 8 days changing between seedling, when changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivated, interface is muffled with the black cloth of one piece of width 20cm, length 40cm, with writing brush to put in worm beaker gently small brown rice planthopper brush is entered below newly to educate on rice seedling, mark on beaker with black marking pen and put the worm date, just complete with the sealing of gauze rubber band and change seedling process.If the worm below the small brown rice planthopper changed 4 age, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worms more than 4 ages of changing, then old seedling will stay growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, general from changing the little nymph of white can seen after Miao Zhi counts 7 days and rice seedling occur successively hatch, within 10 days, teleonymph is hatched completely substantially, then treats nymph to be changed to according to quantity distribution principle for 2-3 days on seedling and newly to educate on rice seedling normal management and raise.
The selection of above embodiment temperature (table 1), intensity of illumination (table 2) and stocking density (table 3) is to rice seedling growing way, survival period based on different condition; Small brown rice planthopper to be grown, goes through more preferably the selecting of making of research of phase, survival rate, Life of Adult and the impact such as ratio, egg hatching rate.
Table 1 different temperatures is on the impact of rice seedling and small brown rice planthopper indices
* note: " going through the phase at the beginning of rice seedling " refers to that rice growing can be used for raising the number of days of small brown rice planthopper to growing to 6cm; " rice seedling survival period " refers to from placement small brown rice planthopper to the number of days changing seedling.
Table 2 different illumination intensity is on the impact of rice seedling and small brown rice planthopper indices
* same table 1 is annotated
The different stocking density of table 3 is on the impact of rice seedling and small brown rice planthopper indices
* same table 1 is annotated
The impact (table 1) that the indices tool of condition of different temperatures on rice seedling and small brown rice planthopper is certain, temperature is shorter and shorter from going through the phase at the beginning of 22-30 DEG C of visible rice seedling, but the survival period of rice seedling decreases because temperature is too high 30 DEG C time, the indices observing small brown rice planthopper more as seen when temperature is 24,26 and 28 DEG C the survival rate in small brown rice planthopper each worm age, Life of Adult, female worm ratio and egg hatching rate all a little more than other temperature, so select the temperature condition of 24-28 DEG C, both can ensure growing fine of rice seedlings, viability and the fertility of small brown rice planthopper can be ensured again.The impact of difference on small brown rice planthopper indices of intensity of illumination is less, and mainly on the impact (table 2) of rice growth, intensity of illumination is higher, goes through that the phase is shorter and survival period is longer at the beginning of rice seedling, so select intensity of illumination to be 5000-7000LUX.On the impact of the survival period of rice seedling and small brown rice planthopper also comparatively large (table 3), when the stocking density of 1-2 instar is below 400/glass, its survival rate is more than 60% for different stocking densities; When the stocking density of 3-4 instar is below 300/glass, its survival rate is more than 73%; When the stocking density of 5-6 instar is below 200/glass, its survival rate is more than 81%, under the different stocking densities in several worm age, its ovum biography rate is all very high, consider the stocking density determining 3 batches of different worm ages to be respectively: the stocking density 300-400 head/cup of 1-2 instar, the stocking density 200-300 head/cup of 3-4 instar, the stocking density 100-200 head/cup of 5-6 instar.
Utilize the present invention to raise small brown rice planthopper, not by the restriction of natural conditions, manual control supports worm condition, and worm scale is supported in adjustment at any time, has both met the demand of experiment, and can not cause waste again.The small brown rice planthopper raised can be applicable to insect and pass the various aspects of virus research.
Above-mentioned enforcement does not limit the present invention in any form.
Claims (1)
1. the method for an indoor mass rearing small brown rice planthopper, it is characterized in that: in the insectary of the certain condition of culture of tool, educate rice seedlings with 1000mL glass beaker refinement earth culture, treat that seedling grows to about 6cm for raising small brown rice planthopper, small brown rice planthopper raises three batches by worm age simultaneously, raises about 10 glasss small brown rice planthopper nymphs for first batch, raise about 10 glasss 3-4 small brown rice planthoppers in age for second batch, raise about 10 glasss striatellus imagos for 3rd batch, a seedling is changed changing between seedling every 8-10 days, when changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on new nursery beaker, with one piece of width 20cm, interface muffled by the black cloth of length 40cm, with writing brush to put in worm beaker gently small brown rice planthopper brush is entered below newly to educate on rice seedling, mark on beaker with black marking pen and put the worm date, again with the sealing of gauze rubber band, period, rice seedlings normally watered management, abundant small brown rice planthopper in quantity sufficient and worm age can be obtained at any time, the key of the method is: 1) culture of pests condition: temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX, 2) first batch of density of raising nymph is 300-400 head/cup, and second batch of density of raising 3-4 instar is 200-300 head/cup, and the 3rd batch of density of raising adult is 100-200 head/cup, 3) change seedling changing between seedling and carry out, change between seedling be between one with place the independent room of raising small brown rice planthopper and separating completely, 4) nursery and raise small brown rice planthopper process in strictly control the amount of watering, add water in soil during sowing moistening, after planting water a water every 48h, be as the criterion so that soil is moistening, the rice seedlings of raising small brown rice planthopper generally waters a water every 48h, and the amount of watering is as the criterion with complete wetting native in cup.
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| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
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