CN105638579A - Method for inducing laodelphax striatellus nymph reproductive diapause - Google Patents
Method for inducing laodelphax striatellus nymph reproductive diapause Download PDFInfo
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- 241001470017 Laodelphax striatella Species 0.000 title claims abstract description 68
- 230000005058 diapause Effects 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000001939 inductive effect Effects 0.000 title claims abstract description 21
- 241000819999 Nymphes Species 0.000 title abstract description 13
- 230000001850 reproductive effect Effects 0.000 title abstract 4
- 235000013601 eggs Nutrition 0.000 claims abstract description 32
- 238000011419 induction treatment Methods 0.000 claims abstract description 26
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 7
- 238000009395 breeding Methods 0.000 claims abstract description 4
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- 241000238631 Hexapoda Species 0.000 claims description 28
- 238000005286 illumination Methods 0.000 claims description 21
- 238000011161 development Methods 0.000 claims description 12
- 241001498622 Cixius wagneri Species 0.000 claims description 9
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- 206010021033 Hypomenorrhoea Diseases 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 claims 1
- 230000006698 induction Effects 0.000 abstract description 12
- 230000002265 prevention Effects 0.000 abstract description 8
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- 235000007164 Oryza sativa Nutrition 0.000 description 16
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pest prevention and control, and particularly relates to a method for inducing laodelphax striatellus nymph reproductive diapause. The method for inducing the laodelphax striatellus nymph reproductive diapause comprises the steps that during matching spawning of laodelphax striatellus female and male adults, light breeding is conducted, light induction treatment is further conducted on the treated laodelphax striatellus eggs directly, or after the eggs grow to 1-3-year-old nymphs, light induction treatment is conducted, and the reproductive diapause phenomenon of laodelphax striatellus can be effectively induced. Meanwhile, it is displayed through data that the diapause rate of the laodelphax striatellus nymphs can reach up to 100% by reasonably controlling the dimming ratio of the light induction processes of the two times.
Description
Technical Field
The invention belongs to the technical field of pest control, and particularly relates to a method for inducing diapause of Laodelphax striatellus nymphs.
Background
Laodelphax striatellus belongs to Laodelphax striatellus of Hemiptera family, and the main distribution areas are southern Hainan island, northern Hainan river, Oriental Taiwan province and eastern coastal areas, west Xinjiang and middle and lower reaches of Yangtze river and North China. The hosts of the laodelphax striatellus are various lawn grasses and gramineous plants such as rice, wheat, corn, barnyard grass and the like, the laodelphax striatellus can not only eat or harm grain crops (such as rice, wheat, barley, corn, sorghum and the like) and economic crops (sugarcane), but also overwinter on various gramineous weed plants such as alopecurus, barnyard grass, Leersia hexandra, paspalum distichum and the like in winter, and can spread various virus diseases to cause the general prevalence of diseases, thereby bringing huge loss to agricultural production.
The adult and nymphs of the laodelphax striatellus all take the rice juice which is absorbed by the mouthparts to be harmful, and generally gather in the upper leaves of rice clusters, in recent years, it is found that the damage of the rice ears of part of rice areas is serious, when the mouths of the laodelphax striatellus are large, the rice juice is largely lost and withered, meanwhile, a large amount of honeydew is scattered on the nearby leaves or ears to breed mold, but the symptoms of 'louse burning' and 'cold piercing' similar to the brown planthopper and the white back planthopper are rarely appear. The laodelphax striatellus is a medium for transmitting various rice virus diseases such as stripe disease and the like, the caused damage is often larger than the direct smoking damage, and the damaged plants show corresponding disease characteristics. Especially, in recent years, the harm of the laodelphax striatellus is gradually increased, secondary pests are increased to be main pests, the harm degree is increased, the harm range is enlarged, the drug resistance of the laodelphax striatellus is gradually enhanced along with the unreasonable and irregular use of a large amount of pesticides, the prevention and control difficulty of the laodelphax striatellus is increased, and the grain safety is seriously affected.
At present, the control modes of the laodelphax striatellus mainly comprise the following steps: 1. agricultural control: selecting an insect-resistant rice variety, performing scientific fertilizer and water management, and creating ecological conditions which are not beneficial to breeding and propagation of the sogatella furcifera; 2. biological control: the method has the advantages that the predatory natural enemies of the laodelphax striatellus are fully utilized to control the harm of the laodelphax striatellus, and the obvious effect can be achieved; 3. chemical control: according to the type of rice variety and the occurrence condition of plant hoppers, a control strategy for mainly controlling the peak period of main-generation low-age nymphs is adopted, and if the adult migration amount is particularly large and concentrated in the year and the area, a strategy for combining the control of the peak migration imagoes and the peak period of the main-generation low-age nymphs is adopted. However, the conventional methods have the problems of long prevention and control period, unstable prevention and control or large residual quantity of reagents, easy pollution to crops and the like.
The diapause of insects refers to the phenomenon that when insects are stimulated by certain signals of adverse environmental changes, the growth, development and reproduction of the insects are induced to stop through a series of coding processes of physiological and biochemical changes in vivo. Diapause of insects helps to help insects spend adverse environments, maintain the survival of populations and individuals; meanwhile, the method is also beneficial to the regular development of insect groups, and the mating probability between male and female individuals is enhanced, so that more offspring can be generated, and the breeding of the seeds is ensured; therefore, diapause of insects is of great significance to the continuation of insect species. The research of human beings on the diapause of insects provides an extremely important theoretical basis for reasonably utilizing beneficial insects and killing pests for human beings, and also provides a new idea for preventing and controlling the Laodelphax striatellus.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a method for inducing diapause of laodelphax striatellus, further define the diapause insect state and diapause induction conditions of the laodelphax striatellus, and provide a basic theory for comprehensive prevention and control of the laodelphax striatellus and more effectively reduce the loss of the laodelphax striatellus to agricultural production.
In order to solve the technical problem, the method for inducing diapause of the nymphaea canicola comprises the following steps:
(1) in the pairing spawning period of the male and female adults of the laodelphax striatellus, illumination feeding is carried out, and the photoperiod light-dark ratio is controlled to be L14-16: d8-10;
(2) carrying out light induction treatment on the eggs of the Laodelphax striatellus, and controlling the photoperiod light-dark ratio to be L8-13.5: d10.5-16, namely diapause of the nymphaea gray planthopper; or,
(2') after the eggs to be treated develop to nymphs, carrying out light induction treatment, and controlling the photoperiod light-dark ratio to be L8-13.5: d10.5-16, namely diapause of the nymphaea gray planthopper.
In the step (1), in the light raising step, the light-to-dark ratio L15 of the photoperiod is controlled: D9.
in the step (1), the light-irradiation feeding step is carried out at a temperature of 24-27 ℃ and a humidity of 70-80%.
In the step (1), the illumination intensity in the illumination feeding step is 10000 lx.
In the steps (2) and (2'), in the light induction treatment step, the light-dark ratio of the control photoperiod L8-11: d13-16.
In the steps (2) and (2'), the step of the light induction treatment is carried out at the temperature of 20 +/-1 ℃ and the humidity of 70-80%.
In the steps (2) and (2'), the light intensity in the step of the light induction treatment is 10000 lx.
In the step (2'), the nymphs are nymphs with the development age of 1-3 years.
The invention also discloses application of the method for inducing diapause of the Laodelphax striatellus nymphs in the field of prevention and control of the Laodelphax striatellus insect pests.
The method for inducing diapause of the nymphs of the laodelphax striatellus is characterized in that the nymphs of the laodelphax striatellus are induced to diapause in a light induction mode through the influence of a photoperiod and temperature. The test result shows that in the method, the laodelphax striatellus eggs are firstly subjected to illumination induction treatment, and the treated laodelphax striatellus eggs are further directly subjected to secondary illumination induction, or secondary illumination induction is carried out after the eggs grow to 1-3-year nymphs, so that the laodelphax striatellus nymphs can be effectively induced to generate diapause. Meanwhile, data show that the diapause rate of the nymphaea planthopper nymphs can be up to 100% by reasonably controlling the light-dark ratio of the two-time illumination induction process.
According to the method, the diapause condition of the Laodelphax striatellus nymphs is induced by determining the diapause state of the Laodelphax striatellus nymphs and screening diapause induction conditions, the diapause characteristic of the Laodelphax striatellus is further determined, a theoretical basis is provided for comprehensive prevention and control of the Laodelphax striatellus and more effective reduction of the loss of the Laodelphax striatellus to agricultural production, and the method has important significance for prevention and control of the Laodelphax striatellus.
Detailed Description
Example 1
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 25 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L16: d8, light intensity is 10000 lx. Directly transferring the treated eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for light induction treatment, and controlling the photoperiod light-dark ratio to be L8: d16, the illumination intensity is 10000lx, and the diapause condition of the Laodelphax striatellus nymphs can be generated.
Example 2
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 26 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L14: d10, light intensity is 10000 lx. Directly transferring the treated eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for illumination induction treatment, and controlling the photoperiod light-dark ratio to be L13.5: d10.5, the illumination intensity is 10000lx, so that the diapause condition of the Laodelphax striatellus nymphs can be generated.
Example 3
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 25 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L15: d9, light intensity is 10000 lx. Directly transferring the treated eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for light induction treatment, and controlling the photoperiod light-dark ratio to be L10: d14, the illumination intensity is 10000lx, and the diapause condition of the Laodelphax striatellus nymphs can be generated.
Example 4
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 25 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L16: d8, light intensity is 10000 lx. Directly transferring the treated eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for light induction treatment, and controlling the photoperiod light-dark ratio to be L12: d12, the diapause condition of the Laodelphax striatellus nymphs can be generated.
Example 5
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 25 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L16: d8, light intensity is 10000 lx. When the eggs grow to 3-year-old nymphs, transferring the processed nymph eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for light induction treatment, and controlling the photoperiod light-dark ratio to be L11: d13, the illumination intensity is 10000lx, and the diapause condition of the Laodelphax striatellus nymphs can be generated.
Example 6
Healthy male and female adults are taken to lay eggs in a small pot of plastic insect cage (fresh rice seedlings are planted in the small pot of plastic insect cage), then, the eggs are subjected to light induction treatment under the environment with the temperature of 25 +/-1 ℃ and the humidity of 70-80%, and the photoperiod light-dark ratio is controlled to be L14: d10, light intensity is 10000 lx. When the eggs grow to 1-year-old nymphs, transferring the processed nymph eggs to an environment with the temperature of 20 +/-1 ℃ and the humidity of 70-80% for light induction treatment, and controlling the photoperiod light-dark ratio to be L12: d12, the illumination intensity is 10000lx, and the diapause condition of the Laodelphax striatellus nymphs can be generated.
Comparative example 1
The method for inducing diapause of the nymphaea planthopper nymphs in the comparative example is the same as that in example 1, the difference is only that in the step of carrying out secondary light induction on the eggs, the photoperiod light-dark ratio is controlled to be L16: D8.
comparative example 2
The method for inducing diapause of the nymphaea planthopper nymphs in the comparative example is the same as that in example 2, the difference is only that in the step of carrying out secondary light induction on the eggs, the photoperiod light-dark ratio is controlled to be L14: D10.
comparative example 3
The method for inducing diapause of the nymphs of the laodelphax striatellus in the comparative example is the same as that in example 5, and the difference is that secondary illumination treatment under the same condition is carried out when the nymphs grow to 4 years old after the eggs are treated.
Examples of the experiments
Judging whether the laodelphax striatellus is diapause according to the development duration of the laodelphax striatellus nymphs: when the nymph development period is more than 36 days, the nymph development period is a diapause group, on the contrary, the nymph development period is less than 26 days, the nymph development period is intermediate between the nymph development period and the nymph development period, namely 26-36 days, the nymph development period is an incomplete diapause group, and the diapause rate is calculated as the number of diapause insects/the total number of insects by 100 percent.
The results of the measurements of diapause status of the laodelphax striatellus nymphs in examples 1 to 6 and comparative examples 1 to 3 are shown in table 1 below.
TABLE 1 results of diapause of Laodelphax striatellus nymphs treated in examples 1-6 and comparative examples 1-3
| Treatment of | Number of test insects | Diapause rate | Average developmental calendar period (d) |
| Example 1 | 50 | 78.6％ | 39.05 |
| Example 2 | 50 | 52.2％ | 33.73 |
| Example 3 | 50 | 100％ | 55.07 |
| Example 4 | 50 | 66.2％ | 35.23 |
| Example 5 | 50 | 72.5％ | 38.24 |
| Example 6 | 50 | 56.3％ | 33.45 |
| Comparative example 1 | 50 | 10.5％ | 22.74 |
| Comparative example 2 | 50 | 30.1％ | 25.32 |
| Comparative example 3 | 50 | 26.3％ | 23.45 |
The test results show that in the method, the laodelphax striatellus ova are subjected to illumination induction treatment firstly, and the treated laodelphax striatellus ova are further subjected to secondary illumination induction directly, or secondary illumination induction is performed after the ova develop to 1-3 instar nymphs, so that the laodelphax striatellus nymphs can be effectively induced to generate diapause. Meanwhile, data show that the diapause rate of the nymphaea planthopper nymphs can be up to 100% by reasonably controlling the light-dark ratio of the two-time illumination induction process.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (9)
1. A method for inducing diapause of Laodelphax striatellus nymphs is characterized by comprising the following steps:
(1) in the pairing spawning period of the male and female adults of the laodelphax striatellus, illumination feeding is carried out, and the photoperiod light-dark ratio is controlled to be L14-16: d8-10;
(2) carrying out light induction treatment on the eggs of the Laodelphax striatellus, and controlling the photoperiod light-dark ratio to be L8-13.5: d10.5-16, namely diapause of the nymphaea gray planthopper; or,
(2') after the eggs to be treated develop to nymphs, carrying out light induction treatment, and controlling the light-dark ratio of the light period to be L8-13.5: d10.5-16, namely diapause of the nymphaea gray planthopper.
2. The method for inducing diapause of the Laodelphax striatellus nymphs according to claim 1, wherein in the step (1), in the light rearing step, the light-dark ratio of a photoperiod is controlled to be L15: D9.
3. the method for inducing diapause of the Laodelphax striatellus nymphs according to claim 1 or 2, wherein in the step (1), the light breeding step is carried out at a temperature of 24-27 ℃ and a humidity of 70-80%.
4. The method for inducing diapause of the Laodelphax striatellus nymphs according to any one of claims 1-3, wherein in the step (1), the illumination intensity in the illumination feeding step is 10000 lx.
5. The method for inducing diapause of the Laodelphax striatellus nymphs according to any one of claims 1 to 4, wherein in the steps (2) and (2'), in the light induction treatment step, the light-dark ratio of the photoperiod is controlled to be L8-11: d13-16.
6. The method for inducing diapause of Laodelphax striatellus nymphs according to claim 5, wherein in the steps (2) and (2'), the step of light induction treatment is performed at a temperature of 20 +/-1 ℃ and a humidity of 70-80%.
7. The method for inducing diapause of Laodelphax striatellus nymphs according to claim 5 or 6, wherein in the steps (2) and (2'), the light intensity in the step of the light induction treatment is 10000 lx.
8. The method for inducing diapause of the Laodelphax striatellus nymphs according to any one of claims 5-7, wherein in step (2'), the nymphs are nymphs with a development age of 1-3 years.
9. Use of the method of inducing diapause of the nymphs of laodelphax striatellus according to any one of claims 1 to 8 in the field of controlling the insect pests of laodelphax striatellus.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012476A (en) * | 2014-06-25 | 2014-09-03 | 江苏省农业科学院 | Method for massively raising laodelphax striatellus indoors |
| CN104255669A (en) * | 2014-09-24 | 2015-01-07 | 中国农业科学院植物保护研究所 | Diapause induction method of harmonia dimidiata |
-
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- 2016-02-15 CN CN201610085893.3A patent/CN105638579A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012476A (en) * | 2014-06-25 | 2014-09-03 | 江苏省农业科学院 | Method for massively raising laodelphax striatellus indoors |
| CN104255669A (en) * | 2014-09-24 | 2015-01-07 | 中国农业科学院植物保护研究所 | Diapause induction method of harmonia dimidiata |
Non-Patent Citations (3)
| Title |
|---|
| 张爱民等: "灰飞虱的种群特性及其与温度的关系", 《昆虫知识》 * |
| 朱文超: "灰飞虱不同地理种群滞育特性差异的比较研究", <中国优秀硕士论文全文数据库> * |
| 贺媛: "灰飞虱的发生规律及滞育特性研究", 《中国优秀硕士论文全文数据库》 * |
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