CN106613131A - Method for producing seed of amorphophallusbulbifer - Google Patents

Method for producing seed of amorphophallusbulbifer Download PDF

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Publication number
CN106613131A
CN106613131A CN201611001170.7A CN201611001170A CN106613131A CN 106613131 A CN106613131 A CN 106613131A CN 201611001170 A CN201611001170 A CN 201611001170A CN 106613131 A CN106613131 A CN 106613131A
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China
Prior art keywords
konjak
ditch
corm
sandy soil
konjac
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CN201611001170.7A
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Chinese (zh)
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余磊
赵建荣
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Kunming University
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Kunming University
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Priority to CN201611001170.7A priority Critical patent/CN106613131A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0291Planting receptacles specially adapted for remaining in the soil after planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Fertilizers (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The patent application discloses a method for producing seeds of amorphophallusbulbifer.The method comprise following steps: A. Konjac corms of Gold konjak variety are selected to conduct Induction flowering treatment; B. the konjac corms processed by step A are put into a biodegradable film bag and sandy soil at a moisture content of 60 to 70% is loaded in the film bag; C. Implant ditch, a layer of 2- 3cm thick rotten organic fertilizer is laid in the ditch , a layer of 3-5cm thick humus is covered on the organic fertilizer , 4 / 5 following parts of the B processing steps after planting konjac in humidity range of 40 50% ditch; then cover the manure with a layer of 3-5cm thick humus. 4 / 5 of the konjac corm processed by the B step is planted in a planting ditch with a humidity range of 40 to 50%; D. period of konjac corm flowering and fruit growth, the environment temperature is controlled within 8 DEG C-30 DEG C, the air relative humidity is 60- 85%, the daytime light intensity is in the range of 2000- 20000 lux; E. after flowering, fruit growth period is more than 180 days, the seeds are harvested and preserved when the color of all the seeds in fruit is red or yellow. By adopting the method mentioned above, the propagation coefficient of konjak is effectively increased, and the production cost of konjak cultivation is greatly reduced.

Description

A kind of method with regard to the bulbil konjak production of hybrid seeds
Technical field
The invention belongs to new plant or obtain the method field of new plant, and in particular to a kind of with regard to the bulbil konjak production of hybrid seeds Method.
Background technology
Konjaku is Araeceae Amorphophallus herbaceos perennial, be find so far uniquely can in a large number synthesize Glucomannan Plant population, be mainly distributed on the torrid zone and subtropical zone in Asia and Africa, there is a cultivation in only minority Asian countries, China, Japan is more universal.The konjaku kind of scale plantation both at home and abroad is elephant-foot yam, white konjaku and its Hybrid of the two, is male and female same Strain, the generative propagation mode of cross-pollination, it is extremely low to bear seeds success rate, and typically less than 2%.Traditional konjaku breeding coefficient only has 3- 6 times, can only be bred using little konjak corm or breeding root (stem) substantially, making the raw material production of konjaku planting industry becomes system The about bottleneck of industry development.
Araeceae konjaku platymiscium, after the growth of 3-5, part konjak corm occurs the phenomenon bloomed. Konjaku blossoms and has seeds process for generative propagation, its crossbreeding same plant male and female pistil self-pollination under natural environmental condition Because gynoecium precocity causes homophyletic infertility, and the successful probability of different strain hybridization is extremely limited, causes konjaku fruit maturation rate extremely low, opens Flower knot maturing rate is less than 2%, and the chance that obtain seed is very low, therefore, konjaku can not usually adopt doing for knot of blooming Method is to carry out production sowing by growing directly from seeds bulb and stripping and slicing in production obtaining the seed of batch, and this is also traditional konjaku Breeding coefficient only has 3-6 times of main cause, current people also carrying out tissue culture quick breeding research, but, so far still So because its efficiency is low, high cost without being applied in produce reality (Wu Jin equalitys, 2007).
Golden konjaku (A.muelleri) is in recent years a kind of wild with what is found in country in Southeast Asia and China's China-Myanmar border one The domestication breeding kind of konjaku.Its stem tuber is rich in the total of glucomannans, moisture of the fresh tuber comprising 75-80% and 20-25% (Glucomannan 78% or so, starch, colloid, oligosaccharides, disaccharide and monose etc. are about 12%) for dry, wherein carbohydrate 90.2%, ash content 4.0%, protein 3.3%, fiber 1.7%.And plant strain growth blade face gas green-ball stem aborning, with many The strong excessively front seedling of seedling and floral leaf symbiosis after seedling relay growth potential and growth potential, 39 pairs of triploids and by present invention side The do not pollinate modes of reproduction of i.e. amixis of method process produces a large amount of seed kinds, the low unique biological characteristics of stem tuber water content, It is set to possess the characteristics of high tolerance to diseases, high yield, growing area low altitude area, and resistance to storage and easily transport.
The content of the invention
It is an object of the invention to provide a kind of method with regard to the bulbil konjak production of hybrid seeds, the method can have in agricultural production Effect ground increases the breeding coefficient of konjaku, and the production cost in konjaku plantation is greatly lowered.
To reach above-mentioned purpose, the base case of the present invention is as follows:
A kind of method with regard to the bulbil konjak production of hybrid seeds, comprises the following steps that:
A. the konjak corm for choosing 1-4 lifes, disease-free wound and weight in more than 200g gold konjaku kinds carries out induced flowering Process, i.e.,:With containing 1ppm auxin (IAA):120-1200ppm gibberellin (GA3)=1:The mixed solution of 1 volume ratio is sprayed On the konjak corm surface of more than 200g, moisten to anhydrous oozing now;
B. the konjak corm after the process of step a is fitted into degradable bag film, and it is 60- to load humidity in bag film 70% wasteland sandy soil, and wasteland sandy soil parcel konjak corm is made, the Jacket thickness of wasteland sandy soil is more than 3cm;
C. select to open plantation ditch in soil or sandy soil mixture, and the thick corruption of one layer of 2-3cm is first applied in plantation ditch Ripe fertilizer, then humus lid last layer 3-5cm thick on fertilizer, the konjak corm after b step is processed Less than 4/5 partly transplants in the plantation ditch of humidity range 40-50%;
D. konjak corm florescence and fruit growth period, it is 60- to control environment temperature for 8 DEG C -30 DEG C, relative air humidity 85%th, daylight intensity is in the range of 2000-20000 luxs;
E. the fruit growth period of Post flowering is more than 180 days, when the color of all seed kinds in fruit is red or yellow Harvest preservation.
The production of hybrid seeds is carried out using this programme there is advantages below:
1st, using this programme producing method for seed breeding coefficient mean height up to 500 times, and with low cost, the few spy of land used Point, can be greatly lowered the production cost in konjaku plantation.
2nd, in stepb, using degradable bag film, and it is 60-70% in konjak corm outer wrapping humidity, thickness is The wasteland sandy soil of more than 3cm, it has been found that konjak corm has higher adaptability in the environment of wasteland sandy soil, and, The resistance against diseases in its later stage can be improved, so as to improve the breeding coefficient for increasing konjaku;And, using the process side of this step Formula, is prevented from the auxin, the gibberellin that spray rapidly by outside soil absorption, makes the work of auxin, gibberellin on konjaku It is longer with the time;Furthermore, under the protective effect of degradable bag film, just plantation konjak corm sprout is valid protected, no Can voluntarily degrade in the presence of microorganism afterwards for a period of time by the pest infestation in soil, and degradable bag film, Will not be to environment;In addition, under the protection of the wasteland sandy soil in degradable bag film and bag, in planting process not Konjak corm surface can be caused to damage.
3rd, in step c, one layer of fertilizer for becoming thoroughly decomposed and one layer of humus are applied, the battalion that abundance can be provided for konjak corm Foster environment, due in fertilizer may non-complete fermentation, zymophyte may be contained, may burn konjak corm, so using One layer of fertilizer is first applied, then humus lid last layer 3-5cm thick on fertilizer, it is ensured that fertilizer provides the same of nutrient When, konjak corm will not be damaged.
Prioritization scheme 1, the further optimization to base case, the bag film is using the maize straw after crushing as base Matter, and sunflower straw, legume stalk after crushing are added as additive, according to the mass fraction, matrix:Additive= 8:2-3, sunflower straw:Legume stalk=1:1.Biodegradable fiber containing high-load element in maize straw, but also The nutrient of plant growth is benefited containing other, as matrix, the bag film later stage can be made degradable;Konjak corm early stage pair Potassium element, nitrogen demand it is higher, and the potassium element containing high level in sunflower straw contains in legume stalk The nitrogen of high level, adopts above-mentioned raw materials that nutrient content can not only be provided for konjak corm, and with green, it is environmentally friendly Advantage.
Prioritization scheme 2, the further optimization to base case, in step c, 1-1.5 month before konjak corm plantation, Interplanting has corn.Konjaku is a kind of half negative plant, and adopting the mode of interplanting corn can provide masking for konjaku, so as to protect The fast-growth of card konjaku.
Prioritization scheme 3, to base case, prioritization scheme 1,2 any one further optimization, in step c, plant ditch two The corn of side interplanting is interplanted towards the inclined mode of plantation ditch.Interplanted using above-mentioned inclined mode, it is possible to increase interplanting corn When to konjaku masking effect, so as to ensure the fast-growth effect of konjaku.
Specific embodiment
Below by specific embodiment, the present invention is further detailed explanation:
It is described in detail by taking embodiment 1 as an example below, other embodiment and comparative example embody in table 1, non-body in table 1 The part revealed is same as Example 1.
Embodiment 1
A kind of method with regard to the bulbil konjak production of hybrid seeds, comprises the following steps that:
A. the konjak corm for choosing 1-4 lifes, disease-free wound and weight in more than 200g gold konjaku kinds carries out induced flowering Process, i.e.,:With containing 1ppm auxin (IAA):120-1200ppm gibberellin (GA3)=1:The mixed solution of 1 volume ratio is sprayed On the konjak corm surface of more than 200g, moisten to anhydrous oozing now;
B. the konjak corm after the process of step a is fitted into degradable bag film, and loads humidity in bag film and be 60% wasteland sandy soil, and wasteland sandy soil parcel konjak corm is made, the Jacket thickness of wasteland sandy soil is 3cm or so;Bag film is adopted With the maize straw after crushing as matrix, and add sunflower straw, legume stalk after crushing as additive, press Mass fraction meter, matrix:Additive=8:2, sunflower straw:Legume stalk=1:1.
C. select to open plantation ditch in soil or sandy soil mixture, and first apply that one layer of 3cm is thick to become thoroughly decomposed in plantation ditch Fertilizer, then humus lid last layer 5cm thick on fertilizer, the konjak corm after b step is processed 4/5 with Transplant in the plantation ditch of humidity range 40-50% lower part;Plant first 1 month or so in konjak corm, interplanting has corn.
D. konjak corm florescence and fruit growth period, it is 60- to control environment temperature for 8-30 DEG C, relative air humidity 85%th, daylight intensity is in the range of 2000-20000 luxs;
E. the fruit growth period of Post flowering is more than 180 days, when the color of all seed kinds in fruit is red or yellow Harvest preservation.
Table 1
It is respectively adopted above example 1-3, the method production of hybrid seeds of comparative example 1-5, to breeding coefficient during the production of hybrid seeds, the production of hybrid seeds During konjaku plant disease condition and upgrowth situation carry out observation statistics, obtain the data of table 2 below:
Table 2
Experiment conclusion:
1st, in table 2 in embodiment 1-3 konjaku breeding coefficient, disease condition and upgrowth situation are superior to comparative example 1-5, from And can be seen that and there is great advantage using the present invention program production of hybrid seeds, additional embodiment 1 is preferred embodiment.
2nd, embodiment 1 can be seen that breeding coefficient compared with comparative example 1 and upgrowth situation has a clear superiority, and both Difference be that embodiment employs auxin and gibberellin and carries out induced flowering process, so induced flowering is processed can effectively carry High breeding coefficient and upgrowth situation.
3rd, embodiment 1 can be seen that breeding coefficient, disease condition and upgrowth situation compared with comparative example 2 and be superior to contrast Example 2, and both differences are that embodiment 1 employs step b, it can thus be seen that during step b can be to the konjaku production of hybrid seeds Breeding coefficient, disease condition and upgrowth situation have very big improvement.
4th, embodiment 1 can be seen that breeding coefficient, disease condition and upgrowth situation compared with comparative example 3 and be superior to contrast Example 2, and both difference to be breeding coefficient, disease condition and upgrowth situation be superior to comparative example 3, and both differences are Embodiment 1 employs step c, it can thus be seen that step c can to the breeding coefficient during the konjaku production of hybrid seeds, disease condition and Upgrowth situation has very big improvement.
5th, embodiment 1 can be seen that breeding coefficient, disease condition and upgrowth situation compared with comparative example 4 and be superior to contrast Example 4, and it is using humus organic fertilizer again is first applied, it can thus be seen that fertilising order meeting that both differences are comparative example 4 There is considerable influence to breeding coefficient, disease condition and upgrowth situation.
6th, embodiment 1 compared with comparative example 5 as can be seen that breeding coefficient and upgrowth situation are superior to comparative example 5, and both Difference be the non-interplanting corn of comparative example 5, it will thus be seen that interplanting corn is conducive to carrying for breeding coefficient and upgrowth situation It is high.
7th, embodiment 1 can be seen that breeding coefficient, disease condition and upgrowth situation compared with comparative example 5 and be superior to contrast Example 5, and both difference sandy soil adopt common sandy soil in b the step of be comparative example 6, it will thus be seen that adopting in step b Wasteland sandy soil are conducive to improving the breeding coefficient of konjaku, disease condition and upgrowth situation.
Above-described is only embodiments of the invention, and the general knowledge here such as known characteristic does not make excessive description in scheme. It should be pointed out that for a person skilled in the art, without departing from the inventive concept of the premise, some changes can also be made Shape and improvement, these should also be considered as protection scope of the present invention, and these are all without the effect and patent for affecting the present invention to implement Practicality.This application claims protection domain should be defined by the content of its claim, the specific embodiment party in specification Formula etc. records the content that can be used for explaining claim.

Claims (4)

1. a kind of method with regard to the bulbil konjak production of hybrid seeds, it is characterised in that comprise the following steps that:
A. the konjak corm for choosing 1-4 lifes, disease-free wound and weight in more than 200g gold konjaku kinds carries out induced flowering process, I.e.:With containing 1ppm auxin(IAA):120-1200ppm gibberellin(GA3)=1:The mixed solution of 1 volume ratio is sprayed in 200g Konjak corm surface above, moistens to anhydrous oozing now;
B. the konjak corm after the process of step a is fitted into degradable bag film, and it is 60-70% to load humidity in bag film Wasteland sandy soil, and make wasteland sandy soil parcel konjak corm, the Jacket thickness of wasteland sandy soil is more than 3cm;
C. select to open plantation ditch in soil or sandy soil mixture, and first apply in plantation ditch that one layer of 2-3cm is thick to become thoroughly decomposed Fertilizer, then humus lid last layer 3-5cm thick on fertilizer, the konjak corm after b step is processed 4/5 with Transplant in the plantation ditch of humidity range 40-50% lower part;
D. konjak corm florescence and fruit growth period, control environment temperature for 8 DEG C -30 DEG C, relative air humidity be 60-85%, Daylight intensity is in the range of 2000-20000 luxs;
E. the fruit growth period of Post flowering is more than 180 days, is adopted when the color of all seed kinds in fruit is red or yellow Receive preservation.
2. a kind of method with regard to the bulbil konjak production of hybrid seeds according to claim 1, it is characterised in that the bag film is adopted Maize straw after crushing adds sunflower straw, legume stalk after crushing as additive as matrix, by matter Amount number meter, matrix:Additive=8:2-3, sunflower straw:Legume stalk=1:1.
3. a kind of method with regard to the bulbil konjak production of hybrid seeds according to claim 1, it is characterised in that in step c, in evil spirit While taro bulb is planted, interplanting has corn.
4. a kind of method with regard to the bulbil konjak production of hybrid seeds according to claim 3, it is characterised in that in step c, plantation The corn of ditch both sides interplanting is interplanted towards the inclined mode of plantation ditch.
CN201611001170.7A 2016-11-14 2016-11-14 Method for producing seed of amorphophallusbulbifer Pending CN106613131A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107172993A (en) * 2017-05-15 2017-09-19 昆明学院 Bulbil konjak seed kind is bred and bulb regeneration method for planting
CN107711371A (en) * 2017-09-29 2018-02-23 陈培党 A kind of porous plantation iron beautiful millettia root structure and its process
CN108323272A (en) * 2018-02-06 2018-07-27 云南省热带作物科学研究所 A kind of method of method and breeding seedling bulb suitable for Xishuangbanna hot-zone bulbil Huang konjaku induced flowering
CN109548607A (en) * 2018-12-14 2019-04-02 湖北美荷农业科技有限公司 A kind of nutritive cube cultural method of konjaku bulbil

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107172993A (en) * 2017-05-15 2017-09-19 昆明学院 Bulbil konjak seed kind is bred and bulb regeneration method for planting
CN107711371A (en) * 2017-09-29 2018-02-23 陈培党 A kind of porous plantation iron beautiful millettia root structure and its process
CN108323272A (en) * 2018-02-06 2018-07-27 云南省热带作物科学研究所 A kind of method of method and breeding seedling bulb suitable for Xishuangbanna hot-zone bulbil Huang konjaku induced flowering
CN109548607A (en) * 2018-12-14 2019-04-02 湖北美荷农业科技有限公司 A kind of nutritive cube cultural method of konjaku bulbil

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