CN110558128A - Industrial root seedling breeding method for hybrid paper mulberry - Google Patents
Industrial root seedling breeding method for hybrid paper mulberry Download PDFInfo
- Publication number
- CN110558128A CN110558128A CN201910813449.2A CN201910813449A CN110558128A CN 110558128 A CN110558128 A CN 110558128A CN 201910813449 A CN201910813449 A CN 201910813449A CN 110558128 A CN110558128 A CN 110558128A
- Authority
- CN
- China
- Prior art keywords
- seedlings
- root
- seedling
- paper mulberry
- laterite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for breeding seedlings by hybridizing broussonetia papyrifera industrial roots, and relates to the technical field of cultivation and breeding of broussonetia papyrifera. The method comprises the following steps: a. pruning lateral roots of the hybrid paper mulberry into root segments of 4-6cm, placing the root segments in 200-300 times of aqueous solution of carbendazim for sterilization for 5min, and drying in the air to obtain explants; b. and cutting the explant in a seedling culture medium, watering, covering a film, and culturing for 30-40 days in an environment with the temperature of 27 ℃ and the air humidity of 60% to obtain root-breeding seedlings. When the method is used for industrial root propagation seedling raising of the paper mulberry, seedlings can emerge at 34.67 +/-0.88 days, and the rate of emergence is as high as 92.35 +/-2.35%; through statistics of the growth conditions of the hybrid paper mulberry after the seedlings are transplanted for 3 months, the quality of seedlings emerging in the short time of root propagation is slightly lower than that of stem section cutting seedlings, but the seedling quality can be consistent with that of the stem section cutting seedlings after the strong seedling time is prolonged.
Description
Technical Field
The invention belongs to the technical field of cultivation and breeding of paper mulberry, and particularly relates to a seedling breeding method for hybrid paper mulberry industrial roots.
Background
The paper mulberry is named Broussonetia papyrifera, belongs to the genus Broussonetia of the family Moraceae, and is also named as paper mulberry, grain wood, grain pulp tree and the like. The paper mulberry is distributed in north China, south China, southwest China and northwest China, and is very common in southern areas. The paper mulberry is a strong positive tree species and has strong adaptability, stress resistance and contamination resistance. The paper mulberry can resist toxic gases such as sulfur dioxide, hydrogen fluoride, chlorine and the like, and can be used as greening tree species in deserts, remote areas and seriously polluted factories; it can also be used as street tree and paper making. The protein content of the broussonetia papyrifera leaves is up to 20-30%, and the broussonetia papyrifera leaves are also rich in amino acids, vitamins, carbohydrates, trace elements and other nutritional ingredients, and can be used for producing complete livestock and poultry feeds after scientific processing.
The hybrid broussonetia papyrifera is cultivated by utilizing a traditional hybrid breeding mode and combining a modern biotechnology means to obtain multipurpose fast-growing tree species with outstanding stress resistance, drought resistance, barren resistance and saline-alkali resistance, can be harvested in the same year of planting, can germinate in the next year, can be harvested for multiple times every year, and belongs to high-yield tree species. The root system of the hybrid paper mulberry is shallow, the lateral roots are developed, the underground root system network of the hybrid paper mulberry can resist surface runoff, control water and soil loss and prevent land desertification, and the hybrid paper mulberry can rapidly afforest barren mountains, barren slopes, barren beaches and saline-alkali lands.
The artificial cultivation method of paper mulberry includes three methods of tissue culture, seeding and stem cutting. The tissue culture technology of the hybrid paper mulberry is mature, and the seedlings can be rapidly bred in batches by the tissue culture rapid propagation technology, but the method is established on the basis of certain equipment conditions and skilled biological operation technology storage, and is not beneficial to reducing the production cost of the seedlings and the application of basic units with simple and crude equipment. The germination rate of the artificial seeding of the broussonetia papyrifera seeds is only 4%, and the offspring can be subjected to character separation and cannot maintain the excellent characters of the female parent. The stem section has strong tolerance in the cuttage season, roots are slow, the current requirements of large-area cultivation and popularization are difficult to meet, and the trunk of the cuttage seedling at the later stage is easy to be hollow and has weak growth vigor.
Disclosure of Invention
In view of the above, the present invention provides a method for industrially root-breeding seedlings of a broussonetia papyrifera hybrid, which can promote rapid differentiation and growth of root segments, can realize seedling breeding of the broussonetia papyrifera hybrid without excessive and complicated treatment, is simple and efficient, has a high survival rate, and can realize continuous production throughout the year.
In order to achieve the above object, the present invention provides the following technical solutions:
The invention provides a method for breeding seedlings by industrial roots of a hybrid paper mulberry, which comprises the following steps: a. pruning lateral roots of the hybrid paper mulberry into root segments of 4-6cm, placing the root segments in 200-300 times of aqueous solution of carbendazim for sterilization for 5min, and drying in the air to obtain explants;
b. And cutting the explant in a seedling culture medium, watering, covering a film, and culturing for 30-40 days in an environment with the temperature of 27 ℃ and the air humidity of 60% to obtain root-breeding seedlings.
Preferably, the diameter of the root section in step a is greater than 3 mm.
Preferably, during the cuttage in the step b, the height of the explant exposed out of the seedling substrate is 0.4-0.6 cm, and the row spacing of the cuttage plants is (2.5-3.5) cm x (4-6) cm. And (3) sterilizing by using 200-300 times of aqueous solution of carbendazim.
Preferably, the thickness of the seedling raising substrate is 8-12 cm.
preferably, the seedling culture medium comprises the following raw materials of laterite, rice chaff ash and calcium superphosphate in a volume ratio of (55-65): (30-40): (2-8).
Preferably, the preparation method of the seedling substrate comprises the following steps: (1) taking the surface layer laterite of the forest land, drying, crushing and sieving by a 2cm sieve to obtain laterite;
(2) And mixing the laterite, the rice chaff ash and the calcium superphosphate according to the volume ratio, and sealing and sterilizing to obtain the seedling culture medium.
Preferably, during the culture process in the step b, water is supplemented every 3 days before the explants grow tender shoots; after the explant grows tender shoots, the plastic film is removed, and water is supplemented once a day.
Preferably, after the seedlings are propagated by the roots in the step b, transplanting the seedlings into a field when the height of the seedlings propagated by the roots reaches 4-6 cm.
The invention provides a method for breeding seedlings by industrial roots of a hybrid paper mulberry, which comprises the following steps: a. pruning lateral roots of the hybrid paper mulberry into root segments of 4-6cm, placing the root segments in 200-300 times of aqueous solution of carbendazim for sterilization for 5min, and drying in the air to obtain explants; b. and cutting the explant in a seedling culture medium, watering, covering a film, and culturing for 30-40 days in an environment with the temperature of 27 ℃ and the air humidity of 60% to obtain root-breeding seedlings. The method can greatly shorten the seedling raising time, greatly increase the rate of emergence, and prolong the strong seedling time, and the quality of the seedlings can be consistent with that of stem cutting seedlings. In the embodiment of the invention, when the method is used for carrying out industrial root propagation and seedling raising on the paper mulberry, seedlings can emerge at 34.67 +/-0.88 d, and the rate of emergence is as high as 92.35 +/-2.35%; through statistics of the growth conditions of the hybrid paper mulberry after the seedlings are transplanted for 3 months, the quality of seedlings emerging in the short time of root propagation is slightly lower than that of stem section cutting seedlings, but the seedling quality can be consistent with that of the stem section cutting seedlings after the strong seedling time is prolonged.
Detailed Description
the invention provides a method for breeding seedlings by industrial roots of a hybrid paper mulberry, which comprises the following steps: a. pruning lateral roots of the hybrid paper mulberry into root segments of 4-6cm, placing the root segments in 200-300 times of aqueous solution of carbendazim for sterilization for 5min, and drying in the air to obtain explants;
b. And cutting the explant in a seedling culture medium, watering, covering a film, and culturing for 30-40 days in an environment with the temperature of 27 ℃ and the air humidity of 60% to obtain root-breeding seedlings.
According to the method, lateral roots of the hybrid broussonetia papyrifera are trimmed to 4-6cm root segments, the root segments are placed in 200-300 times of aqueous solution of carbendazim to be sterilized for 5min, and the explants are obtained after air drying. The lateral roots are preferably selected from the lateral roots of the strong two-year-old hybrid paper mulberry, and the single-side root system is obtained by an excavation method, so that the normal growth of the mother tree is not damaged. The method preferably further comprises the step of shaking the obtained root segments out of the surface soil attached to the root segments before pruning, and the step of maintaining the humidity of the root segments by adopting a water spraying method in the process of pruning the root segments. The diameter of the root section according to the invention is preferably greater than 3 mm. In order to facilitate the simultaneous sterilization of a large number of root segments, the invention preferably bundles the trimmed root segments into bundles with 50 as units and ensures the consistent direction, i.e. one end of each bundle is a morphological upper end and one end is a morphological lower end, and then sterilizes the bundled root segments. The carbendazim is preferably 12.5% synergistic carbendazim wettable powder.
After the explant is obtained, the explant is cut in a seedling culture medium, film is covered after watering, and the root propagation seedling is obtained after culturing for 30-40 days in the environment with the temperature of 27 ℃ and the air humidity of 60%. According to the invention, the obtained explant is subjected to cuttage, and when the explant is subjected to cuttage, the matrix is preferably exposed by 0.4-0.6 cm, and more preferably 0.5 cm. The row spacing of the cuttage plants is preferably (2.5-3.5) cm x (4-6) cm, and more preferably 3cm x 5 cm. Before cuttage, the seedling culture substrate is preferably uniformly paved in a water-leakage plastic box of 40cm multiplied by 60cm, and the thickness is preferably 8-12 cm, and more preferably 10 cm. The cuttings of the present invention are preferably inserted into the substrate vertically with the morphological lower end down so that the root segments are about 0.5cm exposed out of the substrate.
The seedling culture medium preferably comprises laterite, rice chaff ash and calcium superphosphate, wherein the volume ratio of the laterite to the rice chaff ash to the calcium superphosphate is (55-65): (30-40): (2-8).
In the seedling culture medium, the volume ratio of the red loam, the rice chaff ash and the calcium superphosphate is preferably (56-62): (32-38): (3-7), more preferably (58-61): (33-36): (4-6), and most preferably 60:35: 5. The rice chaff ash can loosen soil and increase permeability, and the calcium superphosphate can provide nutrients for a cutting root segment, so that differentiation and growth of the hybrid paper mulberry are promoted. The sources of the rice chaff ash and the calcium superphosphate are not particularly limited, and the rice chaff ash and the calcium superphosphate can be prepared by using conventional raw materials in the field. The laterite is preferably sourced from the laterite on the surface layer of the forest land.
The invention also provides a preparation method of the seedling substrate, which comprises the following steps: (1) taking the surface layer laterite of the forest land, drying, crushing and sieving by a 2cm sieve to obtain laterite;
(2) And mixing the laterite, the rice chaff ash and the calcium superphosphate according to the volume ratio, and sealing and sterilizing to obtain the seedling culture medium.
When the seedling culture medium is prepared, the laterite on the surface layer of the forest land is taken to be dried, crushed and sieved by a 2cm sieve, so that laterite is obtained.
After obtaining the laterite, the invention mixes the laterite, the rice chaff ash and the calcium superphosphate according to the volume ratio, seals and sterilizes to obtain the seedling culture substrate. The sterilization is preferably performed by using 200-300 times of aqueous solution of carbendazim. The carbendazim is preferably 12.5% synergistic carbendazim wettable powder. During the sterilization, the carbendazim diluent and the matrix are fully and uniformly mixed by preferably turning the matrix and spraying, are covered by a plastic film after being piled into a cone shape or are directly filled into a plastic bag and then are sealed for more than 24 hours, and the sterilization is complete.
the method is characterized by watering and film covering after the cuttage, the watering amount is not specially limited, and the preferable watering is carried out until the matrix is completely wetted. The film of the invention can preserve moisture. The invention is preferably carried out in a climatic chamber or a greenhouse after the coating.
In the culture process, the invention also comprises daily management, wherein before the explant grows tender shoots, water is preferably supplemented every 3 days; after the explant grows tender shoots, the plastic film is removed, and water is supplemented once a day.
By using the method, after about 30-40 days after cuttage, the root seedlings grow well, the height of the seedlings reaches 4-6cm, and the seedlings can be transplanted to a field. The transplanting of the invention is preferably to carefully pull out well-grown root seedlings, to avoid hurting the main roots as much as possible, cut 1/3 of the leaves, pack the leaves into boxes and transport the boxes out for transplanting.
The method for breeding seedlings by using industrial roots of a hybrid paper mulberry provided by the invention is described in detail with reference to the following examples, but the method is not to be construed as limiting the scope of the invention.
Example 1
1. Preparing a cutting medium: 1) digging and taking red loam on the surface layer of the forest land, drying, crushing, and screening by a 2cm sieve for later use; 2) fully stirring the laterite, the rice chaff ash and the calcium superphosphate according to the volume ratio of the laterite to the rice chaff ash to the calcium superphosphate of 60:35:5, so that all the components are uniformly mixed to form a cuttage matrix; 3) diluting 12.5% synergistic carbendazim soluble solution with water to 200-300 times of aqueous solution, fully and uniformly mixing the carbendazim diluent with the matrix by a method of turning soil and spraying, piling up into a conical pile, covering with a plastic film or directly filling into a plastic bag, sealing for more than 24 hours, and completely sterilizing for later use.
2. Preparation of root segment: 1) selecting strong lateral roots of the two-year-old hybrid paper mulberry as a material, and obtaining a single-side root system by using an excavation method without damaging the normal growth of a mother tree; 2) the obtained root segments are shaken off the surface soil, the root segments with the diameter of more than 3mm are selected and are trimmed to be about 5 cm; 3) in the root section pruning process, a watering method is adopted to keep the humidity of the root section; 4) bundling the trimmed root sections into bundles by taking 50 as units, and ensuring the consistent directions, namely, one end of each bundle is a morphological upper end, and the other end is a morphological lower end; 5) and (3) soaking the bundled root sections in 200-300 times of water solution diluted by 12.5% of synergistic carbendazim soluble solution and water for 5 minutes, fishing out and airing the root sections until the surfaces of the root sections are dried for later use.
3. root section cuttage: 1) uniformly paving the cuttage matrix in a water leakage plastic box of 40cm multiplied by 60cm, wherein the thickness of the cuttage matrix is about 10 cm; 2) vertically inserting the prepared root segments into the matrix at a plant row spacing of 3cm multiplied by 5cm in a mode that the morphological lower ends face downwards, so that the root segments are exposed out of the matrix by about 0.5 cm; 3) watering until the matrix is completely wetted, covering the plastic box with plastic film to keep moisture, and placing in a climatic chamber or a greenhouse, wherein the temperature is controlled at 27 deg.C and the humidity is 60%.
4. Daily management: 1) supplementing water every three days according to the humidity of the substrate, and observing and counting the germination condition of the root segments. 2) After the tender shoots grow on the root section, the plastic film is removed, and water is sprayed every day to keep the humidity of the matrix.
5. And (3) outplanting of the root seedlings: 1) after about 30-40 days after cuttage, the root seedlings grow well, the height of the seedlings reaches 4-6cm, and the seedlings can be transplanted to a field; 2) carefully pulling out well-grown root seedlings, cutting 1/3 of the leaves, boxing and transporting out for transplanting, wherein the main roots are not damaged as much as possible.
Comparative example 1
The explant selects stem section cutting seedling (JF), stem section propagation is to trim robust branches into 6-8 cm stem sections, the morphological lower end is soaked with hormone (300ppm IBA +400ppm NAA mixed solution) for 1 minute and then is subjected to cutting, the matrix is consistent with the root propagation matrix described in the embodiment 1 and comprises twig cutting (NC) and hard branch cutting (YC), the twigs are branches which grow in the current year and are lignified or incompletely lignified, the hard branches are lignified branches which grow in the current year or more than 1 year, the root sections or the branches are cut into the matrix in a plastic box, then are covered with a plastic film for moisture preservation, and are placed in a climatic chamber with the temperature of 27 ℃ and the humidity of 60% for culture. And under the same management condition, counting the emergence rate and the rooting time. The test results are shown in table 1, wherein GF represents the root-raised seedling conditions of example 1, and the root-raised seedlings can greatly shorten the seedling raising time and greatly increase the rate of emergence.
TABLE 1 comparison of rooting time and emergence rate for different seedling raising modes
| Treatment of | Rooting time (sky) | rate of emergence |
| GF | 34.67±0.88a | 92.35±2.35c |
| NC | 50.67±1.45b | 67.34±3.47a |
| YC | 60.67±1.20c | 81.46±3.08b |
Example 2
Because the root propagation time and the cutting rooting time are different greatly, in order to further compare the seedling quality, four treatments of 60-day transplantation (NC) of twig cuttage, 60-day transplantation (YC) of hard branch cuttage, 40-day transplantation (GF1) of root propagation and 60-day transplantation (GF2) of root propagation are set, the seedlings are transplanted to the same plot according to the plant row spacing of 60cm multiplied by 70cm, the same field management is carried out, the method comprises the steps of applying 1 ton of organic fertilizer to each mu of land before land plowing and weeding once in 1 month, the physical and chemical properties of the soil before seedling transplantation are shown in a table 2, and the growth condition of the hybrid paper mulberry is counted after 3 months of seedling transplantation. The test results are shown in table 3, the plant growth conditions of 60-day transplantation (NC) of shoot cuttage, 60-day transplantation (YC) of hard shoot cuttage and 60-day transplantation (GF2) of root propagation are consistent and slightly superior to those of 40-day transplantation (GF1) of root propagation, which indicates that the quality of seedling emergence in short time of root propagation is slightly lower than that of stem section cuttage seedlings, but the seedling quality can be consistent with that of stem section cuttage seedlings after the strong seedling time is prolonged.
TABLE 2 physicochemical properties of the soil before seedling transplantation
TABLE 3 comparison of plant growth after 3 months of seedling transplantation
The invention provides a method for industrially root-breeding seedlings of a hybrid paper mulberry, which can greatly shorten the seedling raising time, greatly increase the rate of emergence, and prolong the strong seedling time, wherein the quality of seedlings can be consistent with that of stem-section cutting seedlings.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A method for breeding seedlings by hybridizing broussonetia papyrifera industrial roots comprises the following steps: a. pruning lateral roots of the hybrid paper mulberry into root segments of 4-6cm, placing the root segments in 200-300 times of aqueous solution of carbendazim for sterilization for 5min, and drying in the air to obtain explants;
b. and cutting the explant in a seedling culture medium, watering, covering a film, and culturing for 30-40 days in an environment with the temperature of 27 ℃ and the air humidity of 60% to obtain root-breeding seedlings.
2. The method of claim 1, wherein the root section in step a has a diameter greater than 3 mm.
3. the method according to claim 1, wherein the height of the explant exposed out of the seedling substrate in step b is 0.4-0.6 cm, and the row spacing of the cuttage is (2.5-3.5) cm x (4-6) cm. And (3) sterilizing by using 200-300 times of aqueous solution of carbendazim.
4. The method as claimed in claim 3, wherein the thickness of the seedling substrate is 8-12 cm.
5. The method according to claim 3 or 4, wherein the raw materials of the seedling substrate comprise laterite, rice chaff ash and calcium superphosphate, and the volume ratio of the laterite to the rice chaff ash to the calcium superphosphate is (55-65): (30-40): (2-8).
6. The method as claimed in claim 5, wherein the method for preparing the seedling substrate comprises the following steps: (1) taking the surface layer laterite of the forest land, drying, crushing and sieving by a 2cm sieve to obtain laterite;
(2) And mixing the laterite, the rice chaff ash and the calcium superphosphate according to the volume ratio, and sealing and sterilizing to obtain the seedling culture medium.
7. The method of claim 1, wherein during the culturing of step b, the explant is supplemented with water every 3 days before shoots are formed; after the explant grows tender shoots, the plastic film is removed, and water is supplemented once a day.
8. The method according to claim 1, wherein after the root propagation of the seedlings in step b, transplanting the seedlings into a field when the height of the root propagation seedlings reaches 4-6 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910813449.2A CN110558128A (en) | 2019-08-30 | 2019-08-30 | Industrial root seedling breeding method for hybrid paper mulberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910813449.2A CN110558128A (en) | 2019-08-30 | 2019-08-30 | Industrial root seedling breeding method for hybrid paper mulberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110558128A true CN110558128A (en) | 2019-12-13 |
Family
ID=68776846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910813449.2A Pending CN110558128A (en) | 2019-08-30 | 2019-08-30 | Industrial root seedling breeding method for hybrid paper mulberry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110558128A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
| CN113854028A (en) * | 2021-10-12 | 2021-12-31 | 华南农业大学 | Multi-nursery matched seedling raising method based on broussonetia papyrifera root propagation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101379918A (en) * | 2007-09-05 | 2009-03-11 | 北京万富春森林资源发展有限公司 | Stem cottage propagation tree-reconstruction method |
| CN106962059A (en) * | 2017-05-18 | 2017-07-21 | 贵州务川科华生物科技有限公司 | A kind of compound method of paper mulberry transplanting soil matrix |
| CN108651027A (en) * | 2018-05-18 | 2018-10-16 | 广西壮族自治区林业科学研究院 | The method of paper mulberry epicormic branch cuttage |
| CN109122307A (en) * | 2018-10-30 | 2019-01-04 | 江西省科学院生物资源研究所 | A kind of Broussonetia papyrifera seed induction germination accelerating method of irradiation mutagenesis |
-
2019
- 2019-08-30 CN CN201910813449.2A patent/CN110558128A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101379918A (en) * | 2007-09-05 | 2009-03-11 | 北京万富春森林资源发展有限公司 | Stem cottage propagation tree-reconstruction method |
| CN106962059A (en) * | 2017-05-18 | 2017-07-21 | 贵州务川科华生物科技有限公司 | A kind of compound method of paper mulberry transplanting soil matrix |
| CN108651027A (en) * | 2018-05-18 | 2018-10-16 | 广西壮族自治区林业科学研究院 | The method of paper mulberry epicormic branch cuttage |
| CN109122307A (en) * | 2018-10-30 | 2019-01-04 | 江西省科学院生物资源研究所 | A kind of Broussonetia papyrifera seed induction germination accelerating method of irradiation mutagenesis |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
| CN112753393B (en) * | 2020-12-31 | 2022-04-22 | 华南农业大学 | Broussonetia papyrifera root propagation method |
| CN113854028A (en) * | 2021-10-12 | 2021-12-31 | 华南农业大学 | Multi-nursery matched seedling raising method based on broussonetia papyrifera root propagation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103461050B (en) | A kind of loquat downgrades the cultivation method of result ahead of time | |
| CN101637092B (en) | Method for culturing large eucalyptus seedlings | |
| CN104106360B (en) | A kind of pinus khasys grows up the method for select tree epicormic branch cuttage childrenization | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| CN105165512A (en) | Crown inclination controlling four-season cutting rapid propagation method for alnus formosana | |
| CN103053395A (en) | Hole tray substrate for fast cultivation of bonsai semperflorens and use method | |
| CN103858771B (en) | A kind of transplanting method of corn gene plantlet in vitro | |
| CN111972204A (en) | Novel method for cultivating blueberries by using vertical root control container | |
| CN106941937B (en) | Garden construction method for shortening garden construction time of China fir seed garden | |
| CN104838829A (en) | Asexual rapid propagation method for high quality edible Chinese torreya | |
| CN105519402A (en) | A cutting propagation method for jabuticaba | |
| CN103039234B (en) | Winter habit wheat breed summer numerous added-generation method | |
| CN110558128A (en) | Industrial root seedling breeding method for hybrid paper mulberry | |
| CN108605617B (en) | Sowing and seedling raising method for European mountain ash facility | |
| CN108432534A (en) | Pecan tree does sth. in advance the promotion method of result | |
| CN112602522A (en) | Method for grafting germ roots of small camellia oleifera seedlings | |
| CN109566232B (en) | Method for cultivating Chinese wistaria seedlings | |
| CN104303640A (en) | Seedling breeding method of cassava | |
| CN103053312B (en) | Propagation method of Pterocarya stenoptera var. zhijiangensis | |
| CN105359905A (en) | Caryaillinoensis high pressure seedling growing method | |
| CN104272949A (en) | Crassulaceae plant cultivating and seedling method | |
| CN107548858A (en) | A kind of apple nursery stock mating system | |
| CN114402821A (en) | Method for raising seedlings of semi-lignified twigs of agilawood by cutting | |
| CN113973673A (en) | Method for propagating amorphophallus bulbifer seedlings | |
| CN113367043A (en) | Sequoia tsugae cutting medium and seedling raising method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191213 |