CN1810956A - Capsular coating method for micro test tube corm of konjack - Google Patents
Capsular coating method for micro test tube corm of konjack Download PDFInfo
- Publication number
- CN1810956A CN1810956A CN 200610010696 CN200610010696A CN1810956A CN 1810956 A CN1810956 A CN 1810956A CN 200610010696 CN200610010696 CN 200610010696 CN 200610010696 A CN200610010696 A CN 200610010696A CN 1810956 A CN1810956 A CN 1810956A
- Authority
- CN
- China
- Prior art keywords
- test tube
- microballoon stem
- konjaku
- stem
- tube microballoon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The capsulizing and coating method for micro test tube corm of konjack includes the following steps: collecting micro test tube corm of konjack, sterilization, initial coating the micro corm, smoothening the coated micro corm, the secondary coating of the micro corm, capsulizing treatment of the micro corm, the secondary capsulizing treatment, the third capsulizing treatment, inspection, packing with air permeating polyethylene plastic bag and storing. The capsulized and coated micro test tube corm of konjack may be used in large scale plantation of konjack, and the method of the present invention can solve the problems of micro test tube corm of konjack, including dewatering death, short storing period, damage after planting, etc.
Description
Technical field
The present invention relates to konjaku test tube microballoon stem technical field, specifically the capsule coating method of konjaku test tube microballoon stem.
Background technology
Vegetative bulb, whip taro, the root stock of adopting as the cultivation provenance more in the large-scale production of konjaku.These vegetative organs very easily pass to the offspring with pathogenic bacteria, cause that offspring's plant strain growth is slow, sickness rate is high.Utilizing plant tissue culture to cultivate test-tube plantlet can carry out the konjaku breeding, produces no seed culture of viruses taro, recover good strains of seeds.But the breeding coefficient of konjaku tissue cultured seedling is low, production cost is high, transplanting survival rate is low, has limited the application of konjaku tissue cultured seedling.The laboratory that China is relevant utilizes the konjaku tissue culture technique in recent years, has carried out the research of konjaku tissue cultured seedling, konjaku test-tube taro, konjaku smile taro.Chinese patent application CN99121051.4, publication number CN1295790 discloses mass production of konjaku tissue cultured seedling and tissue cultured seedling transplantation technique; Chinese patent application CN02155216.9, publication number CN1422526 discloses the propagation method of konjaku test-tube taro; Chinese patent application CN03135572.2, publication number 1493186 discloses konjaku test-tube taro breeding technology.First patent solved the production in enormous quantities problem of tissue cultured seedling, but the transplanting survival rate of tissue cultured seedling is low, limited the popularization of tissue cultured seedling.Two patents in back have solved the production problem of test-tube taro and the miniature taro of test tube, solved the low difficult problem of transplanting survival rate of konjaku group training product to a certain extent, but be vulnerable to damage when having retention period weak point, cultivation in actual applications, thereby cause the infection of pathogenic bacteria, the effect of group training is descended.Many diseases in the konjaku, particularly present restive soft rot as a kind of soil-borne disease, if planting body sustains damage in cultivation, is very easy to take place soft rot.The rarely seen Chinese patent application CN01115052.1 of patent of the stem tuber of present plant, piece root, bulb, protocorm, publication number CN1391814A, ginger tuber coating agent and preparation method thereof is disclosed, this patent has solved the soft rot and the oxyuriasis of ginger kind stem tuber process to a certain extent, but not effect in the sprouting control of nutrition supply and stem tuber.The present invention is with dressing, two stage processing of capsuleization, not only make konjaku test tube microballoon stem have longer retention period, and the interpolation by nutritive substance, plant hormone and agricultural chemicals, make konjaku test tube microballoon stem have good nutrient environment at the sprouting initial stage, the sprouting cycle that can control and certain disease resistance.
Summary of the invention
The purpose of this invention is to provide a kind of konjaku test tube microballoon stem that makes and have longer retention period, avoid simultaneously in cultivation to test tube microballoon stem damage, avoid the superinfection of pathogenic bacteria, make the capsule coating method of the more simple konjaku test tube of the cultivation microballoon stem of konjaku test tube microballoon stem.
Technical scheme of the present invention is: (alginic acid is received to utilize alginic acid, konjac polysaccharide) solution, calcium chloride solution, gac, starch, derosal (white bacterium is clear) is a raw material, by the program of results konjaku test tube microballoon stem → microballoon stem sterilization → microballoon stem dressing → microballoon stem secondary dressing dressing → microballoon stem smooth surface → dressing test tube microballoon stem capsule processing → microballoon stem capsule secondary treatment → three processing → checks of microballoon stem capsuleization just → ventilative polyethylene plastic bag packing → storage the konjaku group is trained test tube microballoon stem (comprising underground bulb and the gas green-ball stem) parcel that forms and is capsule dressing bulb.The capsule coating method of konjaku test tube microballoon stem carries out according to the following steps:
1) in vitro the various source of inductive konjaku test tube microballoon stem (comprising gas green-ball stem, root test tube microballoon stem) cuts off from the inducing plant body, and sterilizes 5-10 minute at the potassium permanganate solution or the 0.01-0.03% Babysafe aqueous solution of 0.5-1.0%;
2) konjaku test tube microballoon stem takes out from potassium permanganate solution or Babysafe, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal (or m-tetrachlorophthalodinitrile): 10,000 ten thousand unit agricultural streptomycin: fenaminosulf=3-5: 2-6: 0.02-0.05: 0.001-0.006: 0.01-0.07, rolling time are 1-2 minute;
3) after rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes;
4) the konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.05-0.1mg/L, 0.1-0.5mg/L Plant hormones regulators,gibberellins and the naa of 0.02-0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem;
5) after the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, it is comparatively smooth that it is shown;
6) dispose, the smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is 3-10% alginic acid (perhaps alginic acid receive, the konjac polysaccharide) aqueous solution, wherein contains the potassium benzoate of 0.001-0.004%, and solidifying agent is the calcium chloride water of 0.4-0.8%; After the drying, repeat 2-5 time slightly.
7) after the capsule processing, the test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, and plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
The dressing capsule konjaku test tube microballoon stem that takes out from freezer, the bulb that can be used as in the konjaku cultivation uses, and sprouts in after planting 15-30 days.
Mechanism of the present invention is to utilize the adhesive effect of starch under water-soluble condition; gac and sterilant are bonded in the surface of konjaku test tube microballoon stem; form a protective layer to external world; next utilizes the adhesive effect of starch nutritive substance and plant hormone to be bonded in the outside of protective layer; this layer not only provides nutritive substance for the sprouting of konjaku test tube microballoon stem later on; and can provide and break the konjaku dormancy; promote the plant hormone that bulb is taken root; the 3rd utilizes alginic acid to be contained in film forming characteristics under the calcium particle environment; form the dressing test tube microballoon stem of capsuleization; because the brown alga sorrel of capsuleization has certain permeability; not only can stop the moisture loss of konjaku test tube microballoon stem; and can also guarantee the low physiological metabolism of konjaku test tube microballoon stem, guaranteed konjaku test tube microballoon stem keeping at the physiologically active of storage period.Method of the present invention makes konjaku test tube microballoon stem have longer retention period, avoid simultaneously in cultivation to test tube microballoon stem damage, avoid the superinfection of pathogenic bacteria.Make the cultivation of konjaku test tube microballoon stem more simple, reduce the generation of disease simultaneously.
Embodiment
Embodiment 1:
The konjaku test tube microballoon stem of capsule dressing prepares by the following method:
In vitro inductive konjaku test tube microballoon stem cuts off from the inducing plant body under aseptic condition, and at 0.5% disinfecting solution of potassium permanganate 5-10 minute; Konjaku test tube microballoon stem takes out from potassium permanganate solution, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal: 10,000 ten thousand unit agricultural streptomycins: fenaminosulf=3: 2: 0.02: 0.001: 0.01, rolling time was 1-2 minute;
After rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes; The konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.05mg/L, 0.1mg/L Plant hormones regulators,gibberellins and 0.02 when the naa of mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem; After the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, it is comparatively smooth that it is shown; The smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is the 3% alginic acid aqueous solution, wherein contain 0.001% potassium benzoate, solidifying agent is 0.4% calcium chloride water; After the drying, repeat capsule processing 2-5 time slightly.The test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
The dressing capsule konjaku test tube microballoon stem that to preserve in freezer takes out 2500 from freezer, by being seeded in the warmhouse booth of 25 * 35cm, the dressing capsule konjaku test tube microballoon stem of all sowings in after planting 15-30 days is sprouted successively, and the accumulative total germination rate of statistics is 98.5% after 25 days.In sowing, transplant 2500 strains of konjaku test-tube plantlet, add up transplanting survival rate after 25 days, transplanting survival rate is 81.7%.
Embodiment 2:
The capsule dressing of konjaku test tube microballoon stem is undertaken by following:
In vitro inductive konjaku test tube microballoon stem cuts off from the inducing plant body under aseptic condition, and at 1.0% disinfecting solution of potassium permanganate 5-10 minute; Konjaku test tube microballoon stem takes out from potassium permanganate solution, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: m-tetrachlorophthalodinitrile: 10,000 ten thousand unit agricultural streptomycins: fenaminosulf=5: 6: 0.05: 0.006: 0.07, rolling time was 1-2 minute;
After rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes; The konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS solution preparations, the cytokinin that wherein contains 0.1mg/L, 0.5mg/L Plant hormones regulators,gibberellins and the naa of 0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem; After the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, it is comparatively smooth that it is shown; The smooth dressing test tube microballoon stem of handling well is poured on the 60 purpose filter screens, roll in the limit, capsule agent and solidifying agent are sprayed in the limit, carry out the capsule processing of konjaku dressing test tube microballoon stem, the capsule agent is 10% konjac polysaccharide (the perhaps alginic acid is received) aqueous solution, wherein contain 0.004% potassium benzoate, solidifying agent is 0.8% calcium chloride water; After the drying, repeat capsule processing 2-5 time slightly.The test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
Claims (1)
1, a kind of capsule coating method of konjaku test tube microballoon stem is characterized in that carrying out according to the following steps:
1) in vitro the various source of inductive konjaku test tube microballoon stem cuts off from the inducing plant body, and sterilizes 5-10 minute at the potassium permanganate solution or the 0.01-0.03% Babysafe aqueous solution of 0.5-1.0%;
2) konjaku test tube microballoon stem takes out from potassium permanganate solution or Babysafe, roll in Drug coating with roll mode behind the draining a little, the Drug coating composition is an activated carbon: starch: derosal or m-tetrachlorophthalodinitrile: 10,000 ten thousand unit agricultural streptomycin: fenaminosulf=3-5: 2-6: 0.02-0.05: 0.001-0.006: 0.01-0.07, rolling time are 1-2 minute;
3) after rolling finishes, with unnecessary powder filter, finish the elementary dressing of test tube microballoon stem with 60 purpose screen clothes;
4) the konjaku test tube microballoon stem of elementary dressing is poured on the new filter screen, nutritive medium is sprayed onto the test tube microballoon stem surface of elementary Cotton seeds with atomizer, nutritive medium consists of: with the liquid of 2 times MS substratum preparations, the cytokinin that wherein contains 0.05-0.1mg/L, 0.1-0.5mg/L Plant hormones regulators,gibberellins and the naa of 0.02-0.1mg/L, the time of spraying, rolling is 1-2 minute, finishes the secondary dressing of konjaku test tube microballoon stem;
5) after the secondary dressing is finished, dry slightly, after the drying dressing test tube microballoon stem poured on the 60 purpose filter screens and roll, it is comparatively smooth that it is shown;
6) smooth dressing test tube microballoon stem being poured on the 60 purpose filter screens, rolls in the limit, and capsule agent and solidifying agent are sprayed in the limit, and the capsule agent is a 3-10% alginic acid or alginic acid is received or the konjac polysaccharide aqueous solution, wherein contains the potassium benzoate of 0.001-0.004%; Solidifying agent is the calcium chloride water of 0.4-0.8%; After the drying, repeat 2-5 time slightly;
7) after the capsule processing, the test tube microballoon stem of capsuleization is dry slightly, and the capsule test tube microballoon stem that drying is good puts into polyethylene plastic bag, and plastics bag places freezer to preserve, and temperature of ice house is 5-8 ℃, and retention period is 90-200 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100106961A CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100106961A CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1810956A true CN1810956A (en) | 2006-08-02 |
| CN100370021C CN100370021C (en) | 2008-02-20 |
Family
ID=36844077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100106961A Expired - Fee Related CN100370021C (en) | 2006-02-20 | 2006-02-20 | Capsular coating method for micro test tube corm of konjack |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100370021C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379619A (en) * | 2015-10-24 | 2016-03-09 | 施秉县丽康生态农业科技发展有限公司 | Preparation method for test-tube micro-bulbodium of Pinellia ternana |
| CN106561714A (en) * | 2016-11-04 | 2017-04-19 | 昆明学院 | Seed coating for konjac seedling seeds, and preparation method thereof |
| CN106613131A (en) * | 2016-11-14 | 2017-05-10 | 昆明学院 | Method for producing seed of amorphophallusbulbifer |
| US20190021312A1 (en) * | 2015-05-28 | 2019-01-24 | Dümmen Group B.V. | Partially Encapsulated Plant Cuttings |
| CN113016387A (en) * | 2021-04-06 | 2021-06-25 | 何为 | Plastic package method for bulbous flower |
| CN113287520A (en) * | 2021-05-26 | 2021-08-24 | 大连工业大学 | Method for quickly obtaining konjak seedlings |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61170385A (en) * | 1985-01-24 | 1986-08-01 | Tokio Sato | Production of raw material of konjak by cell culture |
| CN1391814A (en) * | 2001-06-19 | 2003-01-22 | 刘建华 | Ginger tuber coating agent and its preparing method |
| CN1203754C (en) * | 2003-08-09 | 2005-06-01 | 云南省农业科学院生物技术与种质资源研究所 | Breeding technology of test-tube konjac |
-
2006
- 2006-02-20 CN CNB2006100106961A patent/CN100370021C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190021312A1 (en) * | 2015-05-28 | 2019-01-24 | Dümmen Group B.V. | Partially Encapsulated Plant Cuttings |
| CN105379619A (en) * | 2015-10-24 | 2016-03-09 | 施秉县丽康生态农业科技发展有限公司 | Preparation method for test-tube micro-bulbodium of Pinellia ternana |
| CN106561714A (en) * | 2016-11-04 | 2017-04-19 | 昆明学院 | Seed coating for konjac seedling seeds, and preparation method thereof |
| CN106561714B (en) * | 2016-11-04 | 2019-05-24 | 昆明学院 | Konjaku grows directly from seeds seed kind seed coat agent and preparation method thereof |
| CN106613131A (en) * | 2016-11-14 | 2017-05-10 | 昆明学院 | Method for producing seed of amorphophallusbulbifer |
| CN113016387A (en) * | 2021-04-06 | 2021-06-25 | 何为 | Plastic package method for bulbous flower |
| CN113287520A (en) * | 2021-05-26 | 2021-08-24 | 大连工业大学 | Method for quickly obtaining konjak seedlings |
| CN113287520B (en) * | 2021-05-26 | 2023-01-10 | 大连工业大学 | Method for quickly obtaining konjak seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100370021C (en) | 2008-02-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN103814821A (en) | Method for efficiently and quickly producing bletilla striata seedling | |
| CN100370021C (en) | Capsular coating method for micro test tube corm of konjack | |
| CN104885936A (en) | Seed liquid culture-based method used for rapid propagation of rhizoma bletillae seedlings | |
| CN103202232B (en) | Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| Zhang et al. | Optimization of nutritional conditions using a temporary immersion bioreactor system for the growth of Bletilla striata pseudobulbs and accumulation of polysaccharides | |
| CN105612859A (en) | Artificial breeding method for tomato seeds | |
| SOUZA et al. | Endophytic bacteria used as bioinoculants in micropropagated banana seedlings | |
| CN102972276A (en) | Method for rapidly exercising field melasma herb tissue culture seedlings | |
| CN107810781A (en) | A kind of method that silkworm living cultivates Cordyceps militaris | |
| CN113016608B (en) | Rooting medium for pigeon pea tissue culture seedlings, application and rooting culture method | |
| CN105340708A (en) | Industrialized seedling production method of panax notoginseng | |
| CN105706570A (en) | Seedling culturing method achieving high germination rate of tomato seeds | |
| CN104738108B (en) | A kind of composition for weaving rooms sterilization | |
| CN109315423B (en) | Dendrobium stem seed germinator and preparation method thereof | |
| CN106718942A (en) | The tissue-culturing rapid propagation and domestication culture techniques of North China's dendrobium candidum | |
| CN109874676A (en) | A kind of fast breeding method of passion fruit detoxic seedling | |
| CN105379619A (en) | Preparation method for test-tube micro-bulbodium of Pinellia ternana | |
| CN105660709B (en) | A kind of method for the accelerating agent of Chinese yew root growth and its for crossbreeding | |
| CN107603885A (en) | A kind of rubber tree powdery mildew in vitro culture method and its culture medium | |
| CN104885933A (en) | Method for culturing polygonum cuspidatum seedlings | |
| CN108782239A (en) | A kind of ginkgo detoxification method for tissue culture | |
| CN104145825A (en) | Stem tip rapid propagation seedling test-tube culturing method for cynara scolymus L. | |
| CN1117857C (en) | Tissue culture method for breeding aloe and its culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080220 Termination date: 20120220 |