CN107211891A - A kind of live fast breeding technique of konjaku callus - Google Patents
A kind of live fast breeding technique of konjaku callus Download PDFInfo
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- CN107211891A CN107211891A CN201710425509.4A CN201710425509A CN107211891A CN 107211891 A CN107211891 A CN 107211891A CN 201710425509 A CN201710425509 A CN 201710425509A CN 107211891 A CN107211891 A CN 107211891A
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- konjaku
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
A kind of live fast breeding technique of konjaku callus, produces callus using plant tissue culture technique induction konjaku, is then directly transplanted to callus in Nutrition Soil, warmhouse booth culture seedling can finally produce the konjac seed of high-quality.Compared to traditional konjac seed mating system, breeding coefficient brings up to the disease incidences such as 70 80 times, and konjak soft rot, southern blight by 46 times and can be controlled within 10%；And compared with traditional konjaku tissue culture method, the live planting percent of callus is up to more than 91%, hence it is evident that higher than konjaku tissue-cultured seedling transplanting survival rate.In addition, the technology also simplify the cumbersome step of traditional konjaku tissue culture, indoor incubation time is reduced, cost has been saved.
Description
Technical field
The present invention is a kind of live fast breeding technique of konjaku callus, belongs to agriculture cultivation technology.
Background technology
Konjaku You Ming Amorphophalus rivieris, are Araeceae Amorphophallus herbaceos perennial.It is sweet containing the Portugal enriched in konjak corm
Glycan, is soluble hemicellulose, the good characteristic such as tool peptization, gelation, film forming and compatibility, is widely used in food
The multiple fields such as product, medicine, industry.
In recent years, with the continuous excavation of konjaku purposes, its market demand increasingly increases, but konjaku breeding coefficient
Low, the disease such as soft rot and southern blight causes a large amount of underproduction of konjaku in addition, or even the trend aggravated year by year is presented.Ganoid konjaku taro, which lacks, to be led
Konjaku processing industry insufficient raw material is caused, the key element as restriction konjaku industry development.It is specific as follows：1. konjaku breeding coefficient
It is low：The breeding coefficient of main breed elephant-foot yam is generally 4-6 times, only produces within 1 year 4-6 root-like stock (ganoid konjaku taro), it is straight that introduces a collection is not enough
Connect the expansion of influence konjaku cultivated area.2. konjac seed is serious in spite of illness：Konjak soft rot and southern blight have a strong impact on konjaku
Grow, crop field konjak soft rot, the southern blight incidence of disease cause a large amount of underproduction of konjaku, or even total crop failure generally more than 30%.
3. konjac seed kind sexual involution：At present, China's cultigen is mainly elephant-foot yam and white konjaku, and mostly farmers' of local group
Kind, varietal character is uneven, adds long-term vegetative propagation, causes variety deterioration, and quality reduction, disease resistance weaken.
The plant tissue culture technique carried out using cellular omnipotency can be obtained substantial amounts of vegetative propagation in a short time and be planted
Strain.However, traditional konjaku group culturation rapid propagating technology incubation is cumbersome, including callus induction, adventitious buds differentiation, adventitious bud life
Multiple links such as root, training tissue culture seedling, transplanting.From technological layer analysis, multiple squamous subculture is also easy to produce the variation of character, and
The transplanting survival rate of konjaku tissue-cultured seedling is relatively low.From economic benefit level analysis, the cost of whole konjaku tissue-culturing rapid propagation is higher, mainly
It is embodied in production link cumbersome, the production cycle is longer.The current technology is not yet applied in production.Therefore, how konjaku is improved
The cost of the survival rate of seedling and reduction tissue culture is current urgent problem to be solved.
The content of the invention
The present invention produces callus using plant tissue culture technique induction konjaku, then directly transplants callus
Into Nutrition Soil, warmhouse booth culture seedling.Konjaku callus induction only needs 30-40 days, and being grown after transplanting 3-4 months can
Produce 15-20g or so high-quality ganoid konjaku taro.Compared to traditional konjac seed mating system, breeding coefficient brings up to 70- by 4-6 times
80 times, and the disease incidence such as konjak soft rot, southern blight can be controlled within 10%；And with traditional konjaku tissue culture method phase
Than the live planting percent of callus is up to more than 91%, hence it is evident that higher than konjaku tissue-cultured seedling transplanting survival rate.In addition, this method is simple
The cumbersome step of traditional konjaku tissue culture is changed, has shortened indoor incubation time, reduce cost.
Technical scheme：
The present invention is achieved by the following technical solutions：
A kind of live fast breeding technique of konjaku callus, is comprised the following steps that：
(1) induction of konjaku callus：Selection health, disease-free konjac seed, are cleaned up with water, dried, entered
Row is disinfected, and the konjak corm disinfected is cut into small pieces, and is cultivated in access culture medium, and the time is 30 days.
(2) rooting induction of konjaku callus：Above-mentioned callus is inoculated into root media and induced, culture 10
Transplanted after it.
(3) the live seedling of konjaku callus：The konjaku callus that above-mentioned induction is handled takes out, and residual is washed with water
Culture medium, sprayed, be transplanted to after drying in Nutrition Soil with 72% 1000 times of liquid of agricultural streptomycin, plastic greenhouse training
Support, additional composite microbiological fertilizer, after the growth 3-4 months, can produce 15-20g or so high-quality ganoid konjaku taro after emerging.
Preferably, the process of disinfecting in the step (1) is that in confined space, every cube is matched with 8ml formaldehyde
5g potassium permanganate mixed fumigation 20min, with 75% alcohol-pickled 30s, 0.1% mercuric chloride immersion 15min, then with aseptic water washing 6
It is secondary.
Preferably, the culture medium prescription in the step (1) is MS+6-BA 1.0mgL-1+NAA 0.5mg·L-1+ sugarcane
Sugared 30gL-1+ agar 4.2gL-1, condition of culture is dark training, and temperature is 28 DEG C, pH5.8-6.0.
Preferably, the konjaku of selection is elephant-foot yam two ages ganoid konjaku taro in the step (1), and weight is 150-250g.
Preferably, konjak corm is cut into 1cm × 1cm × 1cm fritter by the step (1), and every piece of weight is 2-4g.
Preferably, the culture medium prescription in the step (2) is MS+NAA 1.5mgL-1+ sucrose 30gL-1+ agar
4.2g·L-1。
Preferably, the Nutrition Soil in the step (3) is mainly made up of sandy loam, well-rotted farmyard manure, lentinus edodes strain stick, ratio
For 3: 1: 1, " good standing grain man is prosperous " composite microbic bacterial fertilizer additional after emerging, consumption is 30kg/ mus.
Preferably, the plastic greenhouse condition of culture in the step (3) is soil moisture 60%-70%, shade rate 40%-
60%, 20-32 DEG C of temperature, transplant require be depth of planting 10cm, seeding row spacing be 10cm × 20cm, transplant Best Times March-
Between May.
The present invention compared with prior art, has the following advantages that：1. multiple links of traditional tissue culture are reduced, it is only necessary to lure
Callus is given birth in artificial delivery.2. konjaku callus transplantation technique is simpler easy to operate compared to tissue culture transplantation of seedlings.3. konjaku callus
Transplanting survival rate of the live emergence rate of tissue apparently higher than traditional konjaku tissue-cultured seedling.4. the live plumule emergence of konjaku callus
It is middle substantially " leafy relay " phenomenon occur, it is more beneficial for the growth of konjaku underground bulb.
Embodiment
Below by specific embodiment, the present invention is further described, it is noted that for the ordinary skill of this area
For personnel, under the premise without departing from the principles of the invention, some variations and modifications can also be made, these also should be regarded as belonging to
Protection scope of the present invention.
Embodiment 1
A kind of live fast breeding technique of konjaku callus, is comprised the following steps that：
(1) induction of konjaku callus：Selection health, disease-free two age of elephant-foot yam ganoid konjaku taro 150g, (changing herein was
In order to different from embodiment 2), running water is cleaned up, and is dried, is disinfected, in confined space, and every cube is used 8ml formaldehyde
Match 5g potassium permanganate mixed fumigations 20min, 75% alcohol-pickled 30s, 0.1% mercuric chloride immersion 15min, aseptic water washing 6 times；
The konjak corm disinfected is cut into 1cm × 1cm × 1cm fritters, every piece of weight is progress in 2-4g, access MS culture mediums
Culture, culture medium prescription is MS+6-BA 1.0mgL-1+NAA 0.5mg·L-1+ sucrose 30gL-1+ agar 4.2gL-1, training
The condition of supporting is dark training, and temperature is 28 DEG C, pH5.8-6.0.
(2) rooting induction of konjaku callus：Callus is inoculated into Fiber differentiation in root media, culture medium
It is formulated as MS+NAA 1.5mgL-1+ sucrose 30gL-1+ agar 4.2gL-1, cultivating can be transplanted for 10 days.
(3) the live seedling of konjaku callus：The konjaku callus for inducing processing is taken out from bottle, clear water is washed off residual
The culture medium stayed, 72% 1000 times of agricultural streptomycin liquid is sprayed, and is transplanted to after appropriate drying in the Nutrition Soil of preparation, nutrition
Soil is mainly made up of sandy loam, well-rotted farmyard manure, lentinus edodes strain stick, and ratio is 3: 1: 1, plastic greenhouse culture, and soil moisture is kept
In 60%-70%, shade rate 40%-60%, temperature is at 25 DEG C, and depth of planting is 10cm, and seeding row spacing is 10cm × 20cm.Emerge
" good standing grain man is prosperous " composite microbiological fertilizer additional afterwards, growth 3-4 months can produce 15-20g or so high-quality ganoid konjaku taro.
Embodiment 2
A kind of live fast breeding technique of konjaku callus, is comprised the following steps that：
(1) induction of konjaku callus：Selection health, disease-free two age of elephant-foot yam ganoid konjaku taro 250g, running water cleaning
Totally, dry, disinfect, in confined space, every cube matches 5g potassium permanganate mixed fumigation 20min with 8ml formaldehyde,
75% alcohol-pickled 30s, 0.1% mercuric chloride immersion 15min, aseptic water washing 6 times；The konjak corm disinfected is cut into
1cm × 1cm × 1cm fritters, every piece of weight is 2-4g, accesses and is cultivated in MS culture mediums, culture medium prescription is MS+6-BA
1.0mg·L-1+NAA 0.5mg·L-1+ sucrose 30gL-1+ agar 4.2gL-1, condition of culture is dark training, and temperature is 28 DEG C,
pH5.8-6.0。
(2) rooting induction of konjaku callus：Callus is inoculated into Fiber differentiation in root media, culture medium
It is formulated as MS+NAA 1.5mgL-1+ sucrose 30gL-1+ agar 4.2gL-1, cultivating can be transplanted for 10 days.
(3) the live seedling of konjaku callus：The konjaku callus for inducing processing is taken out from bottle, clear water is washed off residual
The culture medium stayed, 72% 1000 times of agricultural streptomycin liquid is sprayed, and is transplanted to after appropriate drying in the Nutrition Soil of preparation, nutrition
Soil is mainly made up of sandy loam, well-rotted farmyard manure, lentinus edodes strain stick, and ratio is 3: 1: 1, plastic greenhouse culture, and soil moisture is kept
In 60%-70%, shade rate 40%-60%, temperature is at 30 DEG C, and depth of planting is 10cm, and seeding row spacing is 10cm × 20cm.Emerge
" good standing grain man is prosperous " composite microbiological fertilizer additional afterwards, growth 3-4 months can produce 15-20g or so high-quality ganoid konjaku taro.
Claims (8)
1. a kind of live fast breeding technique of konjaku callus, it is characterised in that comprise the following steps that：
(1) induction of konjaku callus：Selection health, disease-free konjac seed, are cleaned up with water, dried, disappeared
Poison processing, the konjak corm disinfected is cut into small pieces, and is cultivated in access culture medium, and the time is 30 days.
(2) rooting induction of konjaku callus：Above-mentioned callus is inoculated into root media and induced, after cultivating 10 days
Transplanted.
(3) the live seedling of konjaku callus：The konjaku callus that above-mentioned induction is handled takes out, and the training of residual is washed with water
Base is supported, is sprayed, is transplanted to after drying in Nutrition Soil, plastic greenhouse culture goes out with 72% 1000 times of liquid of agricultural streptomycin
After seedling is topdressed, and after the growth 3-4 months, can obtain 15-20g or so high-quality konjac seed.
2. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that in the step (1)
It is that in confined space, every cube matches 5g potassium permanganate mixed fumigation 20min with 8ml formaldehyde, with 75% to disinfect process
Alcohol-pickled 30s, 0.1% mercuric chloride immersion 15min, then with aseptic water washing 6 times.
3. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that in the step (1)
Culture medium prescription is MS+6-BA 1.0mgL-1+NAA 0.5mg·L-1+ sucrose 30gL-1+ agar 4.2gL-1, cultivate bar
Part is dark training, and temperature is 28 DEG C, pH5.8-6.0.
4. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that selected in the step (1)
The konjaku selected is elephant-foot yam two ages ganoid konjaku taro, and weight is 150-250g.
5. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that the step (1) is by evil spirit
Taro bulb is cut into 1cm × 1cm × 1cm fritter, and every piece of weight is 2-4g.
6. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that in the step (2)
Culture medium prescription is MS+NAA 1.5mgL-1+ sucrose 30gL-1+ agar 4.2gL-1。
7. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that in the step (3)
Nutrition Soil is mainly made up of sandy loam, well-rotted farmyard manure, lentinus edodes strain stick, and ratio is 3: 1: 1, additional composite microbial bacteria after emerging
Fertilizer, consumption is 30kg/ mus.
8. the live fast breeding technique of konjaku callus according to claim 1, it is characterised in that in the step (3)
Plastic greenhouse condition of culture is soil moisture 60%-70%, and shade rate 40%-60%, 20-32 DEG C of temperature is transplanted and requires to broadcast
Depth 10cm is planted, seeding row spacing is 10cm × 20cm, and transplanting Best Times are March-May.
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| CN201710425509.4A CN107211891B (en) | 2017-05-27 | 2017-05-27 | Konjak callus direct seeding and rapid propagation technology |
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| CN201710425509.4A CN107211891B (en) | 2017-05-27 | 2017-05-27 | Konjak callus direct seeding and rapid propagation technology |
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| CN107211891B CN107211891B (en) | 2020-09-04 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094103A (en) * | 2018-01-16 | 2018-06-01 | 安康市农业科学研究所 | A kind of konjaku high yield cultivating method |
| CN109691388A (en) * | 2017-10-21 | 2019-04-30 | 镇江市水木年华现代农业科技有限公司 | A kind of konjaku callus live streaming fast breeding technique |
| CN113100062A (en) * | 2021-04-27 | 2021-07-13 | 安康市农业科学研究院 | Method for obtaining haploid by culturing amorphophallus konjac ovule |
| CN113197096A (en) * | 2021-05-27 | 2021-08-03 | 河南伏牛山百菌园生态农业科技有限公司 | Method for planting konjak by using shiitake mushroom waste stick |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61170385A (en) * | 1985-01-24 | 1986-08-01 | Tokio Sato | Production of raw material of konjak by cell culture |
| JPS61199784A (en) * | 1985-03-01 | 1986-09-04 | Kibun Kk | Cell culture of tuberous root of devil's tongue |
| CN1596618A (en) * | 2004-09-17 | 2005-03-23 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| JP2006271281A (en) * | 2005-03-30 | 2006-10-12 | Gunma Prefecture | Culture medium support consisting essentially of konjak glucomannan and method for utilizing the same |
| CN101543184A (en) * | 2008-03-29 | 2009-09-30 | 恩施土家族苗族自治州农业科学院 | Method for culturing open type tissue of konjak |
| CN102144562A (en) * | 2011-02-24 | 2011-08-10 | 宜昌市农业科学研究院 | Efficient and rapid tissue culture propagation method of konjac |
| CN102640638A (en) * | 2012-04-19 | 2012-08-22 | 重庆市彭水县天娇农业开发有限公司 | Method for breeding seed konjac from konjac corms through segmenting with lateral bud |
| CN103430822A (en) * | 2013-09-09 | 2013-12-11 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN104686350A (en) * | 2015-03-01 | 2015-06-10 | 陈凤佳 | Establishing method for tissue culture and rapid propagation system for amorphophallus konjac |
| CN105503363A (en) * | 2015-12-17 | 2016-04-20 | 济南舜祥医药科技有限公司 | Compound biological bacterial manure special for Konjak and preparation method of compound biological bacterial manure |
| CN105961203A (en) * | 2016-06-15 | 2016-09-28 | 张万萍 | Intermediate propagation method of amorphophallus konjac tissue culture |
-
2017
- 2017-05-27 CN CN201710425509.4A patent/CN107211891B/en not_active Expired - Fee Related
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61170385A (en) * | 1985-01-24 | 1986-08-01 | Tokio Sato | Production of raw material of konjak by cell culture |
| JPS61199784A (en) * | 1985-03-01 | 1986-09-04 | Kibun Kk | Cell culture of tuberous root of devil's tongue |
| CN1596618A (en) * | 2004-09-17 | 2005-03-23 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| JP2006271281A (en) * | 2005-03-30 | 2006-10-12 | Gunma Prefecture | Culture medium support consisting essentially of konjak glucomannan and method for utilizing the same |
| CN101543184A (en) * | 2008-03-29 | 2009-09-30 | 恩施土家族苗族自治州农业科学院 | Method for culturing open type tissue of konjak |
| CN102144562A (en) * | 2011-02-24 | 2011-08-10 | 宜昌市农业科学研究院 | Efficient and rapid tissue culture propagation method of konjac |
| CN102640638A (en) * | 2012-04-19 | 2012-08-22 | 重庆市彭水县天娇农业开发有限公司 | Method for breeding seed konjac from konjac corms through segmenting with lateral bud |
| CN103430822A (en) * | 2013-09-09 | 2013-12-11 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN104686350A (en) * | 2015-03-01 | 2015-06-10 | 陈凤佳 | Establishing method for tissue culture and rapid propagation system for amorphophallus konjac |
| CN105503363A (en) * | 2015-12-17 | 2016-04-20 | 济南舜祥医药科技有限公司 | Compound biological bacterial manure special for Konjak and preparation method of compound biological bacterial manure |
| CN105961203A (en) * | 2016-06-15 | 2016-09-28 | 张万萍 | Intermediate propagation method of amorphophallus konjac tissue culture |
Non-Patent Citations (4)
| Title |
|---|
| 徐小燕等: ""激素对魔芋球茎愈伤组织诱导和植株再生的影响"", 《现代农业科技》 * |
| 王安华等: ""复合微生物菌发酵有机肥在魔芋生产上的应用"", 《湖南农业科学》 * |
| 邹华文等: ""魔芋愈伤组织诱导、分化及植株再生的初步研究"", 《湖北农学院学报》 * |
| 郭政宏等: ""花魔芋组织培养的研究"", 《安徽农业科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109691388A (en) * | 2017-10-21 | 2019-04-30 | 镇江市水木年华现代农业科技有限公司 | A kind of konjaku callus live streaming fast breeding technique |
| CN108094103A (en) * | 2018-01-16 | 2018-06-01 | 安康市农业科学研究所 | A kind of konjaku high yield cultivating method |
| CN113100062A (en) * | 2021-04-27 | 2021-07-13 | 安康市农业科学研究院 | Method for obtaining haploid by culturing amorphophallus konjac ovule |
| CN113100062B (en) * | 2021-04-27 | 2022-12-06 | 安康市农业科学研究院 | Method for culturing and obtaining haploid by utilizing amorphophallus konjac ovule |
| CN113197096A (en) * | 2021-05-27 | 2021-08-03 | 河南伏牛山百菌园生态农业科技有限公司 | Method for planting konjak by using shiitake mushroom waste stick |
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