CN108094215A - Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling - Google Patents
Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling Download PDFInfo
- Publication number
- CN108094215A CN108094215A CN201810092460.XA CN201810092460A CN108094215A CN 108094215 A CN108094215 A CN 108094215A CN 201810092460 A CN201810092460 A CN 201810092460A CN 108094215 A CN108094215 A CN 108094215A
- Authority
- CN
- China
- Prior art keywords
- apple trees
- dwarf stocks
- root
- subculture
- stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
It is as follows the invention discloses a kind of Dwarf Stocks For Apple Trees by tissue cultures obtained from the method for root anvil seedling：1）' the acquisition of the sterile stem-segment with single bud of 1 ' Dwarf Stocks For Apple Trees of Y；2）' 1 ' Dwarf Stocks For Apple Trees of Y just train the acquisition of tender stem；3）' the subculture of 1 ' Dwarf Stocks For Apple Trees of Y；4）' Y 1 ' Dwarf Stocks For Apple Trees are taken root；5）' the hardening of 1 ' Dwarf Stocks For Apple Trees of Y；6）' the transplanting of 1 ' Dwarf Stocks For Apple Trees of Y.The method of the present invention can make ' to train germination rate at the beginning of 1 ' Dwarf Stocks For Apple Trees of Y and reach 75%, Shoot in vitro shoot proliferation rate reaches 45 times, and Shoot in vitro rooting rate reaches more than 85%, and transplanting survival rate reaches 90%.For ' 1 ' Dwarf Stocks For Apple Trees of Y have established technical foundation from smooth promote of rootstock seedling, and for final realization, ' 1 ' Dwarf Stocks For Apple Trees industrial seedling rearings of Y provide reliable technology and theoretical foundation.
Description
Technical field
The present invention relates to fruit nursery stock raising technology fields, are specially that a kind of ' Y-1 ' Dwarf Stocks For Apple Trees are trained by organizing
The method obtained from root anvil seedling is supported, is for explant with Dwarf Stocks For Apple Trees ' Y-1 ', by just training, subculture, takes root, transplants
Step, final ' Y-1 ' the Dwarf Stocks For Apple Trees tissue cultures field-transplanting that obtains is from root anvil seedling.
Background technology
' Y-1 ' Dwarf Stocks For Apple Trees（Original code Y-B030）, it is Research Inst. for fruit Tree, Agricultural Academy of Shanxi Prov.'s apple rootstock
Seminar was since 2001, with Malus wild stock resource Northwest Shanxi Malus baccata（Malus baccata）For material, use
Big group grows directly from seeds breeding technique, regional testing and production practices by 10 years, and the resistance selected is strong, Dwarf Effect on Dwarf is bright
Dwarf Stocks For Apple Trees new varieties aobvious, integral economic trait is excellent, have passed through Shanxi Province's variety of crops in October, 2012 and have examined
Determine committee's authorization.The characteristics such as the stock grafting kind tree body dwarfing, cold-resistant, drought resisting, Early blooming and fruiting, compatibility be good are extremely prominent
Go out, it can be in China North China, northwest, northeast and similar Northwest Shanxi natural conditions cold, arid, the cultivation of barren apple producing region.
For many years, the popularization of ' Y-1 ' Dwarf Stocks For Apple Trees is mainly by intermediate stock form, i.e., first with Malus spectabilis, Malus baccata etc.
Seed plantation obtains base anvil, then grafts ' Y-1 ' dwarfing stock of one section of 15 ~ 30cm on it, finally the Grafted Variety on dwarfing stock.
Using this engrafting method, the characteristic of dwarfing rootstock cannot fully demonstrate, and especially be adapted in tree body dwarfing, fruit quality and tree body
Property aspect performance it is apparent.Moreover, nursery step is grafted once more than conventional seedbed system so that grafting, field nursery stock management program
It is cumbersome, the outplanting cycle is extended, it is time-consuming, it is of high cost.And from the nursery of root anvil have result is early, yield is high, quality is good,
The advantages that seedling raising manners are simple, growing way is consistent, the time limit needed for breeding is shorter, its growth directly to apple tree, bloom, result,
Anti-adversity ability etc. has a direct impact, and is the optimal selection of current high standard building well.It is fast with Apple Culture industry in recent years
Speed development, it is especially prominent from important function of the root anvil in produce reality.China's apple is relatively weak from root anvil raising technology,
As a bottleneck for restricting the good anvil fast development of China's apple nursery stock breeding.
Therefore, the vegetative propagation of ' Y-1 ' Dwarf Stocks For Apple Trees is carried out, realizes the production of its own-rooted tree so that stock
Intrinsic advantage can give full play to, it appears particularly important.The acquisition of Dwarf rootstocks seedling must utilize vegetative propagation technique, and group
Culture technique is knitted as the key technology in vegetative propagation technique, with reproduction speed is fast, breeding coefficient is big, raises up seed neat one
It causes, the merit of original kind can be kept, can be with whole year production, the advantages that high financial profit, is preferentially applied.Meanwhile pin
To being difficult to obtain the stock of own-rooted tree by the vegetative propagation techniques method such as cuttage, press strip, the application of tissue culture technique just into
For unique selection.
The content of the invention
The present invention causes dwarfing rootstock to solve that in the actual popularization of ' Y-1 ' Dwarf Stocks For Apple Trees intermediate stock can only be done
Characteristic cannot give full play to, and extend the outplanting cycle, seedling cost etc. is a series of to restrict ' Y-1 ' Apple Dwarfs so as to improve
Change the bottleneck problem of stock fast development, provide a kind of ' Y-1 ' Dwarf Stocks For Apple Trees by tissue cultures obtained from root anvil seedling
Method.
The present invention adopts the following technical scheme that realization：
A kind of ' Y-1 ' Dwarf Stocks For Apple Trees, obtained from the method for root anvil seedling, are included the following steps by tissue cultures：
（1）, the sterile stem-segment with single bud of ' Y-1 ' Dwarf Stocks For Apple Trees acquisition
The first tenday period of a month in May cut ' Y-1 ' Dwarf Stocks For Apple Trees new life branch from base portion, remove blade and petiole, are packed into freshness protection package
In, carry out sterilization processing；Sterilisation step is：Branch tap water is rinsed into half an hour first, and with suds wash clean；
Branch is cut into stem-segment with single bud, is put into the bottle for filling sterile water, on band to aseptic superclean bench；Secondly, sterile ultra-clean
On workbench, the sterile water being placed in stem-segment with single bud bottle is outwelled, with the small watering can equipped with 70% medicinal alcohol by stem-segment with single bud
It squirts, after 30 seconds, pours into 0.1% HgCI2All stem-segment with single bud can be completely soaked by solution；After five minutes by HgCI2It is molten
Liquid is collected into old soldi, soaked stem-segment with single bud with aseptic water washing three times, it is spare.
（2）, ' Y-1 ' Dwarf Stocks For Apple Trees just train tender stem acquisition
Sterile stem-segment with single bud is contacted into both ends with liquid and respectively cuts away 0.5cm, is implanted into ready just training culture medium；Just training training
Foster base is：MS minimal mediums add plant growth regulator BA 2.0mg/l and IBA 0.5mg/l, put culturing room and trained
It supports；Culturing room's environmental condition is：When light application time 16 is small, 25 DEG C of temperature, humidity 70%；Culture 20 ~ 25 days, bud sprout long 2 ~ 3cm
When, you can carry out subculture.
（3）, ' Y-1 ' Dwarf Stocks For Apple Trees subculture
By step（2）In sprouting bud on superclean bench, cut, be inoculated by sterilizing after being commissioned to train from its base portion with knife
Support base；Subculture medium is：MS minimal mediums add plant growth regulator BA 0.6mg/l, IBA 0.3mg/l and GA3
0.5mg/l；
It is cultivated 25 days in culturing room, simple bud has grown, tiller is into bud clump, continues subculture；Continuous subculture 5 times or more,
A part of material continues subculture, and a part of material carries out culture of rootage；In squamous subculture, the same step of culturing room's condition of culture（2）.
（4）, ' Y-1 ' Dwarf Stocks For Apple Trees take root
By step（3）Middle subculture material height is 2 centimetres or more of tender stem, is cut, is inoculated into scissors on superclean bench
By in the root media of sterilization, putting in culturing room and cultivating；Root media is：1/2MS（Greatly）Culture medium adds plant
1.0 mg/l of growth regulator IBA；The same step of culturing room's environmental condition（2）, it cultivates 20 days, 0.5 ~ 1.0cm of root long, and every plant
Have 3 roots and more than, take and hardening carried out under room scattering light.
（5）, ' Y-1 ' Dwarf Stocks For Apple Trees hardening
Blake bottle equipped with rooted seedling is taken and carries out hardening under Indoor Natural scattering light, at 10 days, bottle cap is opened, injection is sterile
Water, depth 0.8cm, then uncovered continue hardening three days, you can transplanting；
The test tube seedling refined from blake bottle is taken out, is put into the open glass cup for filling tap water, is gently agitated for, by root
The culture medium of attachment is separated with root, is flushed three times repeatedly, and washed test tube seedling is put into the plastic box of lid, transports to arch of transplanting seedlings
It is transplanted in canopy.
（6）, ' Y-1 ' Dwarf Stocks For Apple Trees transplanting
It transplants seedlings and encircles temperature of shed：25 ~ 28 DEG C of daytime, 18 ~ 22 DEG C of evening, humidity 70 ~ 90%, 11 points of fine day shade at 3 points in afternoon
70%；Transplanting medium is turf, vermiculite, perlite mixed-matrix, and matrix is sterilized with 800 times of carbendazim, 50 hole plastics of disk of transplanting seedlings
Hole tray；One plant of test tube seedling is transplanted per cave, after whole disk is transplanted, sprays saturating matrix with water, there is water exudation at cave bottom；
The hole tray transplanted is moved to and is transplanted seedlings in shed, then takes Small plastic shed moisturizing；Spray every three days it is permeable once, one week starts to put
Wind, and start to spray nutrient solution, once a week；Wherein, it is 1 in mass ratio by potassium dihydrogen phosphate and urea that the nutrient solution, which is,:1
The concentration being configured to is 0.5% solution；
Small plastic shed plastic cloth is taken in transplanting for 10 days off, spends one week and hole tray is moved on to outdoor；At one week of outdoor beginning, noon will hide
Shade can transplant crop field after two weeks, carry out Routine Management.
It is significant to note that step（1）In, the materials time is crucial, and early May is best period, and too early branch is too
It is tender, easy browning；Branch degree of lignification is high too late, and difficulty is sheared on superclean bench, and miscellaneous bacteria is more, and pollution rate is high.Explant
HgCI of the body 0.1%2In solution, impregnate no more than five minutes, otherwise, melting brown rate substantially rises.
Step（3）In, subculture number is had to more than five times, and only in this way, rooting rate can be only achieved more than 85%, improve
Emergence efficiency.
Step（4）In, in root media, it is ensured that a great number of elements halves, and the amount of trace element is constant, is ' Y-1 ' apple
The necessary condition that fruit dwarfing rootstock Shoot in vitro is normally taken root.
Step（5）In, bottle seedling of taking root hardening must be scattered indoors under light, and illumination is too strong, and test tube seedling is easily wilted, too
Effect weak and that hardening is not achieved.
Present invention design is reasonable, obtains ' Y-1 ' Dwarf Stocks For Apple Trees from root anvil seedling by tissue culture technique, can overcome
The deficiency of intermediate stock is applied in apple nursery stock breeding, is laid a good foundation to apple standardized management；It is given birth to for rapid, high volume
Production ' Y-1 ' Dwarf Stocks For Apple Trees own-rooted tree has established technology and practical basis, is of great significance.
Specific embodiment
Specific embodiments of the present invention are described in detail below.
A kind of ' Y-1 ' Dwarf Stocks For Apple Trees, obtained from the method for root anvil seedling, are comprised the following steps by tissue cultures：
（1）, the sterile stem-segment with single bud of ' Y-1 ' Dwarf Stocks For Apple Trees acquisition
In the first tenday period of a month in May, in Shanxi, fruit tree institute's Genus Mains Seedling garden, ' Y-1 ' Dwarf Stocks For Apple Trees new life branch is cut from base portion
Under, remove blade and petiole, be fitted into freshness protection package, bring laboratory into and carry out sterilization processing.
Sterilisation step is：Branch tap water is rinsed into half an hour first, and with suds wash clean；Branch is cut into
Stem-segment with single bud is put into the bottle for filling sterile water, on band to aseptic superclean bench.It secondly, will in aseptic superclean bench
The sterile water being placed in stem-segment with single bud bottle is outwelled, and is squirted stem-segment with single bud with the small watering can equipped with 70% medicinal alcohol, about 30 seconds
Afterwards, 0.1% HgCI is poured into2All stem-segment with single bud can be completely soaked by solution；After five minutes by HgCI2Solution is collected into sky
In bottle, soaked stem-segment with single bud with aseptic water washing three times, it is spare.
（2）, ' Y-1 ' Dwarf Stocks For Apple Trees just train tender stem acquisition
Sterile stem-segment with single bud is contacted into both ends with liquid with sterilized scalpel and respectively cuts away 0.5cm, is implanted into ready first
It trains in culture medium.Just training culture medium is：MS minimal mediums add plant growth regulator BA 2.0mg/l and IBA 0.5mg/
L is put culturing room and is cultivated.Culturing room's environmental condition is：When light application time 16 is small, 25 DEG C of temperature, humidity 70%.Culture 20 ~ 25
My god, when bud sprouting is about 2 ~ 3cm, you can carry out subculture.
（3）, ' Y-1 ' Dwarf Stocks For Apple Trees subculture
By step（2）In sprouting bud on superclean bench, cut with scalpel from base portion, be inoculated into the subculture by sterilizing
Culture medium.Subculture medium is：MS minimal mediums, add plant growth regulator BA 0.6mg/l, IBA 0.3mg/l and
GA30.5mg/l.It is cultivated 25 days in culturing room, simple bud has grown, tiller is into bud clump, can continue subculture.Continuously
After subculture 5 times, a part of material continues subculture, and a part of material can carry out culture of rootage.In squamous subculture, culturing room's culture
Conditional synchronization is rapid（2）.
（4）, ' Y-1 ' Dwarf Stocks For Apple Trees take root
By step（3）Middle subculture material height is the tender stem of more than 2cm, is cut on superclean bench with medical scissors, is inoculated with
To in by the root media of sterilization, put in culturing room and cultivate.Root media is：1/2MS（Greatly）（It is big in MS culture mediums
Secondary element halves, and trace element is constant）Culture medium adds plant growth regulator IBA 1.0mg/l.Culturing room's environmental condition is same
Step（2）, cultivate 20 days, 0.5 ~ 1.0cm of root long, and every plant have 3 roots and more than, you can take and carried out under room scattering light
Hardening.
（5）, ' Y-1 ' Dwarf Stocks For Apple Trees hardening
Blake bottle equipped with rooted seedling is taken and carries out hardening under Indoor Natural scattering light, at 10 days, bottle cap is opened, injection is sterile
Water, depth 0.8cm, then uncovered continue hardening three days, you can transplanting.The test tube seedling refined is taken with tweezers from blake bottle
Go out, be put into the open glass cup for filling tap water, be gently agitated for tweezers, the culture medium that root is adhered to is separated with root, instead
It flushes three times again, washed test tube seedling is put into the plastic box of lid, transports in shed of transplanting seedlings and is transplanted.
（6）, ' Y-1 ' Dwarf Stocks For Apple Trees transplanting
Shed of transplanting seedlings has cascade, wind turbine, has mobile sunshade net on canopy, can be created properly to test tube transplantation of seedlings with temperature control, shade
External environment.Encircle temperature of shed, on 25 ~ 28 DEG C of daytime, 18 ~ 22 DEG C at night, humidity 70 ~ 90%, 11 points of fine day hides at 3 points in afternoon
Shade 70%.Transplanting medium is turf, vermiculite, perlite mixed-matrix, and proportions 1:1:1, matrix is gone out with 800 times of carbendazim
Bacterium, 50 hole plastics cave dishes of disk of transplanting seedlings.
One plant of test tube seedling is transplanted per cave.After whole disk is transplanted, saturating matrix is sprayed with water, there is water exudation at cave bottom.It will transplanting
Good hole tray, which is moved to, transplants seedlings in shed, then takes Small plastic shed moisturizing.Spray every three days it is permeable once, one week starts to leak informaton, and starts to spray
Nutrient solution, once a week；Wherein, it is 1 in mass ratio by potassium dihydrogen phosphate and urea that nutrient solution, which is,:1 concentration being configured to is
0.5% solution.Transplanting can take Small plastic shed plastic cloth off in 10 days.It spends one week and hole tray is moved on into outdoor.The one of outdoor beginning
Week, noon will suitably shade, and can transplant crop field after two weeks, carry out Routine Management.
One kind ' Y-1 ' Dwarf Stocks For Apple Trees of the present invention by tissue cultures obtained from the method for root anvil seedling, can be with
' Y-1 ' Dwarf Stocks For Apple Trees is made just to train germination rate and reach 75%, Shoot in vitro shoot proliferation rate reaches 4 ~ 5 times, and Shoot in vitro is taken root
Rate reaches more than 85%, and transplanting survival rate reaches 90%；It is established for ' Y-1 ' Dwarf Stocks For Apple Trees from smooth promote of rootstock seedling
Technical foundation realizes that ' Y-1 ' Dwarf Stocks For Apple Trees industrial seedling rearing provides reliable technology and theoretical foundation to be final.
It should be pointed out that for the those skilled in the art of the art, without departing from the principle of the present invention,
Several improvement and application can also be made, these are improved and application is also considered as protection scope of the present invention.
Claims (3)
1. a kind of Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling, it is characterised in that：Include the following steps：
（1）, the sterile stem-segment with single bud of ' Y-1 ' Dwarf Stocks For Apple Trees acquisition
The first tenday period of a month in May cut ' Y-1 ' Dwarf Stocks For Apple Trees new life branch from base portion, remove blade and petiole, are packed into freshness protection package
In, carry out sterilization processing；
（2）, ' Y-1 ' Dwarf Stocks For Apple Trees just train tender stem acquisition
Sterile stem-segment with single bud is contacted into both ends with liquid and respectively cuts away 0.5cm, is implanted into ready just training culture medium；Just training training
Foster base is：MS minimal mediums add plant growth regulator BA 2.0mg/l and IBA 0.5mg/l, put culturing room and trained
It supports；Culturing room's environmental condition is：When light application time 16 is small, 25 DEG C of temperature, humidity 70%；Culture 20 ~ 25 days, bud sprout long 2 ~ 3cm
When, carry out subculture；
（3）, ' Y-1 ' Dwarf Stocks For Apple Trees subculture
By step（2）In sprouting bud on superclean bench, cut from its base portion, be inoculated into the squamous subculture by sterilizing
Base；Subculture medium is：MS minimal mediums add plant growth regulator BA 0.6mg/l, IBA 0.3mg/l and GA3
0.5mg/l；
It is cultivated 25 days in culturing room, simple bud has grown, tiller is into bud clump, continues subculture；Continuous subculture 5 times or more,
A part of material continues subculture, and a part of material carries out culture of rootage；In squamous subculture, the same step of culturing room's condition of culture（2）；
（4）, ' Y-1 ' Dwarf Stocks For Apple Trees take root
By step（3）Middle subculture material height be more than 2cm tender stem, cut on superclean bench with scissors, be inoculated into through
It crosses in the root media of sterilization, puts in culturing room and cultivate；Root media is：1/2MS（Greatly）Culture medium adds plant life
Long conditioning agent IBA 1.0mg/l；The same step of culturing room's environmental condition（2）, cultivate 20 days, 0.5 ~ 1.0cm of root long, and every plant has 3
A root and more than, take and hardening carried out under room scattering light；
（5）, ' Y-1 ' Dwarf Stocks For Apple Trees hardening
Blake bottle equipped with rooted seedling is taken and carries out hardening under Indoor Natural scattering light, at 10 days, bottle cap is opened, injection is sterile
Water, depth 0.8cm, then uncovered continue hardening three days, you can transplanting；
The test tube seedling refined from blake bottle is taken out, is put into the open glass cup for filling tap water, is gently agitated for, by root
The culture medium of attachment is separated with root, is flushed three times repeatedly, and washed test tube seedling is put into the plastic box of lid, transports to arch of transplanting seedlings
It is transplanted in canopy；
（6）, ' Y-1 ' Dwarf Stocks For Apple Trees transplanting
It transplants seedlings and encircles temperature of shed：25 ~ 28 DEG C of daytime, 18 ~ 22 DEG C of evening, humidity 70 ~ 90%, 11 points of fine day shade at 3 points in afternoon
70%；Transplanting medium is turf, vermiculite, perlite mixed-matrix, and matrix is sterilized with 800 times of carbendazim, 50 hole plastics of disk of transplanting seedlings
Hole tray；One plant of test tube seedling is transplanted per cave, after whole disk is transplanted, sprays saturating matrix with water, there is water exudation at cave bottom；
The hole tray transplanted is moved to and is transplanted seedlings in shed, then takes Small plastic shed moisturizing；Spray every three days it is permeable once, one week starts to put
Wind, and start to spray nutrient solution, once a week；Wherein, it is 1 in mass ratio by potassium dihydrogen phosphate and urea that nutrient solution, which is,:1 is configured to
Concentration be 0.5% solution；
Small plastic shed plastic cloth is taken in transplanting for 10 days off, spends one week and hole tray is moved on to outdoor；At one week of outdoor beginning, noon will hide
Crop field is transplanted in shade after two weeks, carries out Routine Management.
By tissue cultures obtained from the method for root anvil seedling, 2. feature exists Dwarf Stocks For Apple Trees according to claim 1
In：Step（1）In, sterilisation step is：Branch tap water is rinsed into half an hour first, and with suds wash clean；By branch
Item is cut into stem-segment with single bud, is put into the bottle for filling sterile water, on band to aseptic superclean bench；Secondly, in sterile ultra-clean work
On platform, the sterile water being placed in stem-segment with single bud bottle is outwelled, is squirted stem-segment with single bud with the small watering can equipped with 70% medicinal alcohol,
After 30 seconds, 0.1% HgCI is poured into2All stem-segment with single bud can be completely soaked by solution；After five minutes by HgCI2Solution is collected
Into old soldi, soaked stem-segment with single bud with aseptic water washing three times, it is spare.
3. Dwarf Stocks For Apple Trees according to claim 1 or 2 are by tissue cultures obtained from the method for root anvil seedling, feature
It is：Step（6）In, turf, vermiculite, the ratio of perlite are 1 in transplanting medium:1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810092460.XA CN108094215A (en) | 2018-01-31 | 2018-01-31 | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810092460.XA CN108094215A (en) | 2018-01-31 | 2018-01-31 | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108094215A true CN108094215A (en) | 2018-06-01 |
Family
ID=62220730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810092460.XA Pending CN108094215A (en) | 2018-01-31 | 2018-01-31 | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108094215A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548653A (en) * | 2018-12-21 | 2019-04-02 | 江苏大学 | A kind of quick mating system of tissue cultures for the winter red fruit that makes a variation |
| CN109892224A (en) * | 2018-12-24 | 2019-06-18 | 江苏东郁植物科技有限公司 | Apple rootstock " Sprout Free " tissue culture of sprout is commercialized mating system |
| CN114150015A (en) * | 2021-09-07 | 2022-03-08 | 浙江万里学院 | Method for improving apple rootstock green branch cutting rooting through transient transformation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439372A (en) * | 2017-07-11 | 2017-12-08 | 北京市农林科学院 | A kind of quick breeding Dwarf Stocks For Apple Trees C.G 935 method |
| CN107494262A (en) * | 2017-08-28 | 2017-12-22 | 山东省果树研究所 | Tissue culture and rapid propagation method and application and culture medium and preparation method, the disinfection treatment method of apple rootstock bar for apple rootstock |
-
2018
- 2018-01-31 CN CN201810092460.XA patent/CN108094215A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439372A (en) * | 2017-07-11 | 2017-12-08 | 北京市农林科学院 | A kind of quick breeding Dwarf Stocks For Apple Trees C.G 935 method |
| CN107494262A (en) * | 2017-08-28 | 2017-12-22 | 山东省果树研究所 | Tissue culture and rapid propagation method and application and culture medium and preparation method, the disinfection treatment method of apple rootstock bar for apple rootstock |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548653A (en) * | 2018-12-21 | 2019-04-02 | 江苏大学 | A kind of quick mating system of tissue cultures for the winter red fruit that makes a variation |
| CN109892224A (en) * | 2018-12-24 | 2019-06-18 | 江苏东郁植物科技有限公司 | Apple rootstock " Sprout Free " tissue culture of sprout is commercialized mating system |
| CN114150015A (en) * | 2021-09-07 | 2022-03-08 | 浙江万里学院 | Method for improving apple rootstock green branch cutting rooting through transient transformation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104885773A (en) | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries | |
| CN111616052A (en) | Rapid propagation and sugar-free rooting culture method and application of apple rootstock catalpa bungei | |
| CN107926715A (en) | A kind of eggplant or/and the engrafting and cultivating method of capsicum or/and tomato | |
| CN102805035A (en) | Common head cabbage tissue culture method | |
| CN104365318A (en) | Standardized myrtle planting technology | |
| CN110558172A (en) | Strawberry virus-free tissue culture seedling breeding method | |
| CN107018896A (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN102783418B (en) | Tissue culture method for pyrethrum cinerariifolium | |
| CN108094215A (en) | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling | |
| CN106942053B (en) | A kind of tissue culture and rapid propagation method for Xingan lucid asparagus | |
| CN103548692A (en) | Tissue culture rapid propagation method for asplenium antiquum osaka germchit | |
| CN101946704B (en) | Method for regenerating Chinese rose plant by using immature seed as explant | |
| CN109496840A (en) | Huaiyuan white flower jade seed pomegranate clone cultivation technique method | |
| CN106665367B (en) | A kind of Golden Bell Tree quick breeding method for tissue culture | |
| CN111034617B (en) | Method for breeding tea seedlings by culturing young embryo tissues of Yunnan large-leaf tea trees | |
| CN103283504B (en) | Method for grafting pear polyploidy test-tube plantlet outside test tube | |
| CN103039363B (en) | Rooting medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN103430822B (en) | Aquaculture seed reproduction method for micro seed tubers of konjac | |
| CN107683768B (en) | A kind of acclimatization and transplants method of the syringa reticulata var mandshurica tissue-cultured seedling of callus induction | |
| CN103039362A (en) | Subculture medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN106417009A (en) | Fig tissue culture and rapid propagation technology and method thereof | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN1324952C (en) | Quick breeding method for tissue culture of lemon verbena | |
| CN102884980B (en) | Quick tissue propagation method of paphiopedilum bellatulum | |
| CN1327760C (en) | Flower-forcing method for fortunes paulownla seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180601 |
|
| RJ01 | Rejection of invention patent application after publication |