CN109548653A - A kind of quick mating system of tissue cultures for the winter red fruit that makes a variation - Google Patents
A kind of quick mating system of tissue cultures for the winter red fruit that makes a variation Download PDFInfo
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- CN109548653A CN109548653A CN201811569057.8A CN201811569057A CN109548653A CN 109548653 A CN109548653 A CN 109548653A CN 201811569057 A CN201811569057 A CN 201811569057A CN 109548653 A CN109548653 A CN 109548653A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of quick mating systems of tissue cultures of winter red fruit that makes a variation, and belong to Plant Tissue Breeding breeding field.The present invention provides the methods of the selection of explant in variation winter red fruit tissue cultures and disinfection, Initial culture, squamous subculture, culture of rootage and transplantings, to reduce the pollution rate of variation winter red fruit tissue cultures and because of the death rate of tissue-cultured seedling caused by dealing with improperly, variation winter red fruit tissue cultures efficiency is further increased.
Description
Technical field
The invention belongs to Plant Tissue Breeding to breed field, and in particular to a kind of tissue cultures for the winter red fruit that makes a variation are quickly numerous
Educate method.
Background technique
Winter red fruit is rosaceae Malus defoliation small arbor.Blade and vein are in green, and pattern baby pink, spring opens
Put, immediately as a result, fruit tufted, fruit color is just green, after turn yellow, late autumn rear blade falls off, and fruit color is scarlet, great success,
It is not fallen through the winter, until second year 2-3 month, therefore also known as Longevity, thus ornamental value is high.Meanwhile plant is shorter and smaller, tree
Shape is compact, and it is ornamental to be particularly suitable for making potted landscape, puts suitable for indoor and outdoor, deep to be liked by the masses.The breeding of winter red fruit generally mostly uses apple
Fruit seedling makees stock grafting, and second year can yield positive results.Secondly seminal propagation is used, but the time limit needed for result is longer.Mesh
Preceding tissue cultures multiplication technique is also applied to the breeding of winter red fruit on a small quantity.
In recent years, this seminar introduces a fine variety in winter red fruit and has been surprisingly found that a plant mutant plant in reproductive process, and leaf color is with season
Section is in the variation characteristic of purple-green multiple conversions, and vein is purple, and fruit initial stage is also purple, until late autumn fruit color is scarlet, fruit color by
Purple enters red.Features above largely meets people's requirement new to ornamental plant, odd, special, therefore, has this special
The winter red fruit variant of anisotropic shape such as can be achieved holding and the fast breeding of character, certainly will have high market potential.So
And grafting twig of traditional propagation by grafiting method without abundance, and sow gained seedling and apparent trait segregation occur.Therefore,
Carrying out variation winter red fruit breeding using tissue cultures is an effective technical measures just now.
In fact, during making a variation winter red fruit tissue cultures, explant disinfection, hierarchical culture, hardening and transplanting etc.
Many important links are still wait advanced optimize and perfect.The present invention be directed to the above link, carry out a large number of experiments, and then it is condensed go out one
The tissue culture method for breeding of kind variation winter red fruit.
Summary of the invention
Technical problem:
The present invention is intended to provide a kind of quick mating system of tissue cultures for the winter red fruit that makes a variation, can keep the variation winter
The specific traits of red fruit, and a large amount of good variation winter red fruit seedlings can be quickly obtained, to realize high-quality variation winter red fruit kind
The volume production of seedling, meets the needs of market.
Technical solution:
In order to achieve the above object, the invention adopts the following technical scheme: a kind of tissue cultures for the winter red fruit that makes a variation are quick
Mating system carries out as steps described below:
(1) explant selects and cleaning: clip variation branch of the new pumping of winter red fruit with axillary bud is explant, will after segmentation
After it is immersed in washing powder water (5g washing powder is added in the water of 1L) 3-5min, rinse well, then immerse original liquid concentration 27-33g/
L vibrates 3-5min in the benzalkonium bromide solution after water dilutes 15 times, and flowing water rinses 30-60min；
(2) explant sterilizes: sterilizing 5-15min, aseptic water washing 3-5 times, each 2- with 0.1% liquor natrii hypochloritis
5min；
(3) explant sterilized Initial culture: is cut into the stem for being 1-2cm with axillary bud length on superclean bench
Section accesses stem section in bud inducement cultivation base, and cultivation temperature is 24-26 DEG C, light application time 10h/d, intensity of illumination 1800-
2200Lx, wherein the formula of the bud inducement cultivation base is that addition 100-200ml concentration is 1g/L in the MS culture medium of 1L
Roxithromycin mother liquor and 10-15ml concentration be 30g/L nano-TiO2Solution；
(4) squamous subculture: after Initial culture nearly 1 month, in the good clump bud of upgrowth situation, the side separated between bud is taken
Formula separates the tender shoots simple bud that length is 2-3cm, in subculture medium of transferring, wherein the formula of the subculture medium
To add the 6-BA solution that 0.5-2ml concentration is 1g/L in the MS culture medium of 1L, it is as above to cultivate Thermo-Photo sensitive by pH:5-5.5；
(5) culture of rootage: after cultivating nearly 1 month in subculture medium, base portion regenerates clump bud, is more than 2cm by length
Tender shoots separated from bud clump, access root media culture nearly 45 days after, formed rooted seedling, wherein the culture of rootage
The IBA solution that 0.5-2ml concentration is 0.1g/L is added in the MS culture medium that the formula of base is 1L, pH:5-5.5 cultivates warm striation
Part is as above；
(6) it hardening and transplanting: about after one and a half months, is chosen in root media and grows 10 or so roots, plant heights
For the tissue-cultured seedling of 5cm or so stalwartness in hardening 1 week or so in greenhouse, clear water washed away root culture medium, is transplanted to nutrient situation
In the transplanting medium that preferable vegetable garden soil and vermiculite are made into the ratio of 1:1, is cultivated in the environment of wet, shade and use plastics
Thin film moisturizing 10d grows to new root young leaves and gradually removes shade, by the Plant colonization of transplant survival in open country at one month, and
Reinforce fertilizer and water management and the prevention and control of plant diseases, pest control, survival rate is up to 98%.
It in 1962 was tobacco cell Training Design that wherein the MS culture medium, which is Murashige and Skoog, due to
Its scope of application is wider, and most plants tissue-culturing quick-propagation uses it as minimal medium at present, and formula is access
Existing general fixed formula；Wherein the 6-BA solution is 6-benzyl aminopurine solution.
The beneficial effects of the present invention are:
(1) it selects stem section to carry out tissue cultures for explant, the specific traits of variation plant can be kept, with propagation by grafiting
It compares, is not limited by the time, to realize breeding scale breeding, and then realize industrialization development, there is important research and life
Produce practical significance.
(2) disinfection is combined using washing powder, benzalkonium bromide and liquor natrii hypochloritis in disinfection treatment, and controls and disappears
The malicious time, can guarantee as far as possible kill explant surface whole microorganism under the premise of, to plant tissue and cells of superficial layer
It does not generate or only generates slight injury, and avoid using mercuric chloride, it is more environmentally-friendly.
(3) in Initial culture, appropriate roxithromycin and nano-TiO are added in MS culture medium2, can not only reduce fungi and
The pollution of bacterium, and improve the survival rate of explant, bud growing state is also more healthy and stronger, is more advantageous to progress after being commissioned to train
It supports.
(4) in culture of rootage, IBA is added, relatively adding can be to avoid the generation of callus for NAA, and rooting rate is high, root
Also more sturdy, to prepare for hardening；
(5) the variation winter red fruit tissue-cultured seedling rooting rate obtained using method for tissue culture of the invention is high, high survival rate, numerous
It is fast to grow speed, rooting rate there are broader market prospects up to 98% or more, robust growth.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but case study on implementation does not do any form to the present invention
Restriction.Unless stated otherwise, the present invention uses reagent, method and apparatus for the art conventional reagent, method and are set
It is standby.Unless stated otherwise, agents useful for same and material are commercially available.
It is carried out in Jiangsu University, industry tissue culture experiments room using this method, in traditional Malus tissue culture
It is partly improved in method, introduces variation winter red fruit tissue culture propagation, easy to operate, high survival rate mentions
High variation winter red fruit tissue culture propagation efficiency.The winter red fruit quick breeding method for tissue culture implementation steps that make a variation are as follows:
1, explant selection and cleaning
April 15, clip make a variation branch of the new pumping of winter red fruit with axillary bud as explant, laundry are immersed in after segmentation
After powder water (5g washing powder is added in the water of 1L) impregnates 3min, rinses well, then immerse original liquid concentration 27-33g/L, diluted through water
3-5min is vibrated in benzalkonium bromide solution after 15 times, flowing water rinses 30min；
2, explant sterilizes
With 0.1% liquor natrii hypochloritis sterilize 10min, aseptic water washing 5 times, each 2min；
3, Initial culture
The explant sterilized is cut into the stem section for being 2cm containing the length with axillary bud on superclean bench, stem section is connect
Enter in bud inducement cultivation base, cultivation temperature is 25 DEG C, light application time 10h, intensity of illumination 2200Lx, wherein the bud
The formula of Fiber differentiation is the solution that roxithromycin is made into 1g/L in advance, and spare after biofilter filters；Routinely
It is 30g/L nano-TiO that method, which is prepared and 10ml concentration is added in the MS culture medium of 1L,2Solution, in 121 DEG C of temperature, pressure 1.1kg/
Sterilize 20min under the conditions of cm2, and the roxithromycin of 200ml is added before culture medium cooled and solidified on the super-clean bench, divides after shaken well
Dress.15 bottles in total, every bottle of 2 explants, 3 batch weight are multiple, after cultivating 30d, statistics statistics pollution rate (pollution rate=pollution explant
Body number/inoculation explant number × 100%) and adventitious bud induction frequency (adventitious bud induction frequency=explant starts number of sprouting/connects
Uncontaminated number × 100% after kind)；It in 1962 was tobacco cell that wherein the MS culture medium, which is Murashige and Skoog,
Training Design, since its scope of application is wider, most plants tissue-culturing quick-propagation uses it as cultivating substantially at present
Base, formula are formulated to consult existing general fixing.
4, squamous subculture
After Initial culture 30d, the good adventitious bud of growing way is selected, takes the mode separated between bud to be divided into the clump bud of 2cm long
Individual spray, in increment culture medium of transferring, wherein added in the MS culture medium that the formula of the proliferated culture medium is 1L
0.8ml concentration is the 6-BA solution of 1g/L, pH 5.5；After cultivating 30d, log, statistics value-added coefficient (increment system
The sprout number before sprout number/subculture after number=subculture)；The wherein 6-BA solution 6-benzyl aminopurine solution.
5, culture of rootage
After cultivating 30d in proliferated culture medium, base portion regenerates clump bud, and the spray of length 2.5cm is separated from bud clump,
After accessing the nearly 45d of root media culture, rooted seedling is formed, wherein the MS that the formula of the root media is 1L is cultivated
The IBA solution that 0.5ml concentration is 0.1g/L, pH 5.5 are added in base；Count rooting rate (rooting rate=rooted seedling number/total seedling number
× 100%).Wherein the IBA solution is 3- indolebutyric acid solution.
6, hardening and transplanting
After 45d, the tissue-cultured seedling that 10 or so roots, plant height 5cm or so stalwartness are grown in root media is chosen in temperature
Indoor hardening 1 week, clear water washes away root culture medium, and tissue culture transplantation of seedlings is made into vegetable garden soil and vermiculite by the volume ratio of 1:1
Transplanting medium in, wherein nutrient contained by vegetable garden soil used are as follows: available nitrogen 585.82mg/g, rapid available phosphorus 38.52mg/g, speed
Imitate potassium 76mg/g.It is cultivated in the environment of moistening, shading and covers moisturizing 10d with plastic film, grown gradually to new root young leaves
It removes shade, by the Plant colonization of transplant survival in open country when 30d, and reinforces fertilizer and water management and the prevention and control of plant diseases, pest control；Statistics transplanting
Survival rate (transplanting survival rate=deposit total seedling number × 100% of number of live vaccine/transplanting).
7, cultivation results
Using method provided by the invention, the winter red fruit stem section pollution rate that makes a variation is only 4%, value-added coefficient 4.8, adventitious bud
Inductivity, rooting rate and transplanting survival rate reach 98% or more.
Claims (4)
1. a kind of quick mating system of tissue cultures for the winter red fruit that makes a variation, it is characterised in that carry out as steps described below:
(1) explant selects and cleaning: clip variation branch of the new pumping of winter red fruit with axillary bud is explant, is soaked after segmentation
Bubble is rinsed well, then immerse original liquid concentration 27-33g/L, is passed through after washing powder water (5g washing powder is added in the water of 1L) 3-5min
3-5min is vibrated in benzalkonium bromide solution after 15 times of water dilution, flowing water rinses 30-60min；
(2) explant sterilizes: sterilizing 5-15min, aseptic water washing 3-5 times, each 2- with 0.1% liquor natrii hypochloritis
5min；
(3) explant sterilized: being cut into the stem section for being 1-2cm with axillary bud length by Initial culture on superclean bench, will
Stem section accesses in bud inducement cultivation base, and cultivation temperature is 24-26 DEG C, light application time 10h/d, intensity of illumination 1800-
2200Lx, wherein the formula of the bud inducement cultivation base is that addition 100-200ml concentration is 1g/L in the MS culture medium of 1L
Roxithromycin mother liquor and 10-15ml concentration be 30g/L nano-TiO2Solution；
(4) squamous subculture: after Initial culture nearly 1 month, in the good clump bud of upgrowth situation, take the mode separated between bud will
Length is that the tender shoots simple bud of 2-3cm separates, in subculture medium of transferring, wherein the formula of the subculture medium is 1L
MS culture medium in addition 0.5-2ml concentration be 1g/L 6-BA solution, pH:5-5.5, culture Thermo-Photo sensitive it is as above；
(5) culture of rootage: after cultivating nearly 1 month in subculture medium, base portion regenerates clump bud, is more than the tender of 2cm by length
Bud is separated from bud clump, after access root media culture nearly 45 days, forms rooted seedling, wherein the root media
The IBA solution that addition 0.5-2ml concentration is 0.1g/L in the MS culture medium that formula is 1L, pH:5-5.5, culture Thermo-Photo sensitive is such as
On；
(6) it hardening and transplanting: about after one and a half months, is chosen in root media and grows 10 or so roots, the left side plant height 5cm
For the tissue-cultured seedling of right stalwartness in hardening 1 week or so in greenhouse, clear water washed away root culture medium, and it is preferable to be transplanted to nutrient situation
In the transplanting medium that vegetable garden soil and vermiculite are made into the ratio of 1:1, cultivates in the environment of wet, shade and protected with plastic film
Wet 10d grows to new root young leaves and gradually removes shade, by the Plant colonization of transplant survival in open country at one month, and reinforces fertilizer
Water management and the prevention and control of plant diseases, pest control, survival rate is up to 98%.
2. a kind of quick mating system of tissue cultures of winter red fruit that makes a variation according to claim 1, it is characterised in that wherein
It is tobacco cell Training Design that the MS culture medium, which was Murashige and Skoog in 1962, due to its scope of application ratio
Relatively wide, most plants tissue-culturing quick-propagation uses it as minimal medium at present, and formula is consolidated for access is existing general
Fixed formula.
3. a kind of quick mating system of tissue cultures of winter red fruit that makes a variation according to claim 1, it is characterised in that wherein
The 6-BA solution is 6-benzyl aminopurine solution.
4. a kind of quick mating system of tissue cultures of winter red fruit that makes a variation according to claim 1, it is characterised in that wherein
The IBA solution is 3- indolebutyric acid solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110972935A (en) * | 2019-10-16 | 2020-04-10 | 中国农业科学院麻类研究所 | Application of iron-based nanoparticles in promoting growth of plant stem tips |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007121518A1 (en) * | 2006-04-21 | 2007-11-01 | The State Of Western Australia Through Its Department Of Agriculture | Improved plant culture methods using a modified auxin treatment step |
| CN105532482A (en) * | 2016-03-14 | 2016-05-04 | 龙岩市禾康生物科技有限公司 | Antibacterial culture medium for tissue culture of plants and preparation method of culture medium |
| CN105660391A (en) * | 2014-11-17 | 2016-06-15 | 孟三军 | Tissue culture breeding method for apple sapling |
| CN107371880A (en) * | 2017-07-28 | 2017-11-24 | 济南浩隆生物科技有限公司 | A kind of apple rootstock tissue culturing fast seedling-cultivating method |
| CN108094215A (en) * | 2018-01-31 | 2018-06-01 | 山西省农业科学院果树研究所 | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
| CN106937597B (en) * | 2017-04-12 | 2018-11-27 | 山东农业大学 | A kind of tissue-culturing rapid propagation method suitable for high flavonoids apple new lines |
| CN108935100A (en) * | 2018-07-20 | 2018-12-07 | 河南农业大学 | A kind of method of apple rootstock T337 tissue-cultured seedling rooting induction |
-
2018
- 2018-12-21 CN CN201811569057.8A patent/CN109548653A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007121518A1 (en) * | 2006-04-21 | 2007-11-01 | The State Of Western Australia Through Its Department Of Agriculture | Improved plant culture methods using a modified auxin treatment step |
| CN105660391A (en) * | 2014-11-17 | 2016-06-15 | 孟三军 | Tissue culture breeding method for apple sapling |
| CN105532482A (en) * | 2016-03-14 | 2016-05-04 | 龙岩市禾康生物科技有限公司 | Antibacterial culture medium for tissue culture of plants and preparation method of culture medium |
| CN106937597B (en) * | 2017-04-12 | 2018-11-27 | 山东农业大学 | A kind of tissue-culturing rapid propagation method suitable for high flavonoids apple new lines |
| CN107371880A (en) * | 2017-07-28 | 2017-11-24 | 济南浩隆生物科技有限公司 | A kind of apple rootstock tissue culturing fast seedling-cultivating method |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
| CN108094215A (en) * | 2018-01-31 | 2018-06-01 | 山西省农业科学院果树研究所 | Dwarf Stocks For Apple Trees are by tissue cultures obtained from the method for root anvil seedling |
| CN108935100A (en) * | 2018-07-20 | 2018-12-07 | 河南农业大学 | A kind of method of apple rootstock T337 tissue-cultured seedling rooting induction |
Non-Patent Citations (4)
| Title |
|---|
| CHAO-HONG FENG等: ""Duration of sucrose preculture is critical for shoot regrowth of in vitro-grown apple shoot-tips cryopreserved by encapsulation-dehydration"", 《PLANT CELL TISS ORGAN CULTURE 》 * |
| 查仁明: ""苹果属植物组织培养快速繁殖技术"", 《青海农林科技》 * |
| 韦晨: ""变异冬红果光合生理特性与组培技术的研究"", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
| 韦晨等: ""变异冬红果无菌体系的建立"", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110972935A (en) * | 2019-10-16 | 2020-04-10 | 中国农业科学院麻类研究所 | Application of iron-based nanoparticles in promoting growth of plant stem tips |
| CN110972935B (en) * | 2019-10-16 | 2021-07-16 | 中国农业科学院麻类研究所 | Application of iron-based nanoparticles in promoting growth of plant stem tips |
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