CN107018897B - A method of rapid breeding is organized using water lily - Google Patents
A method of rapid breeding is organized using water lily Download PDFInfo
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- CN107018897B CN107018897B CN201710212711.9A CN201710212711A CN107018897B CN 107018897 B CN107018897 B CN 107018897B CN 201710212711 A CN201710212711 A CN 201710212711A CN 107018897 B CN107018897 B CN 107018897B
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- blade
- water lily
- fibrous root
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- plant
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- 241000209490 Nymphaea Species 0.000 title claims abstract description 49
- 235000016791 Nymphaea odorata subsp odorata Nutrition 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000009395 breeding Methods 0.000 title claims abstract description 26
- 230000001488 breeding effect Effects 0.000 title claims abstract description 21
- 241000196324 Embryophyta Species 0.000 claims abstract description 34
- 230000004913 activation Effects 0.000 claims abstract description 16
- 230000008635 plant growth Effects 0.000 claims abstract description 11
- 238000012545 processing Methods 0.000 claims abstract description 7
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 3
- 201000010099 disease Diseases 0.000 claims abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 3
- 230000017074 necrotic cell death Effects 0.000 claims abstract description 3
- 208000024891 symptom Diseases 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 4
- 240000001624 Espostoa lanata Species 0.000 claims description 4
- 238000009400 out breeding Methods 0.000 claims description 4
- 230000034303 cell budding Effects 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- 239000000645 desinfectant Substances 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 239000005648 plant growth regulator Substances 0.000 claims description 2
- 230000014284 seed dormancy process Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a kind of methods using water lily tissue rapid breeding, comprising: chooses the blade with base of leaf joint, blade surface is without necrosis, no disease and pests harm symptom;It carries out cleaning malicious activation processing using base of leaf joint of the plant growth essence activity factor to blade surface, the base of leaf joint director of blade surface goes out tender shoots, after continuing culture 5-10 days, when the tender shoots of blade grows 3-4 piece blade, rhizome is grown below blade, rhizome size 1-3cm is transplanted into Pot for cultivating plant, nest spacing is 10*10 centimeters, and keeping the ambient temperature of Pot for cultivating plant is 20 DEG C -28 DEG C.The present invention can blade to water lily, fibrous root and rhizome carry out Propogation and culture, it is only necessary to control the ambient temperature in cultivation period, and activation processing carried out to the water lily tissue of cultivation, can be realized large-scale plantation cultivation.
Description
Technical field
The present invention relates to water lily nursery culture technology field, especially a kind of method using water lily tissue rapid breeding.
Background technique
Water lily, alias: water lily.The perennial water plant of Nymphaea, fruit are more containing grain weight.Ecological habit, extensively
It is distributed in Asia, Africa and America, there are many designs and varieties, red, yellow, blue, purple, pink, some kinds and aromatic, and temperature is 20
DEG C -30 DEG C are optimum temperature, and bud is unlimited in this temperature range, are easier to be trained New Year flowers.Four
The places such as river, 5-10 month can be most appropriate to wetland park and all water plants greening place, plantation is convenient, has with low level management
Pond is more preferable, does not have pond that can erect the low bank of earth between fields of 30cm high, film on pad, and the fertile soil for being padded with 20cm can plant, easily
Appearance, breeding coefficient is big, and market is broad, it is easy to form industry.
Two kinds of traditional water lily propagation method, only seed and plant division, not using the Cultivating techniques of blade breeding.
Number of patent application: the 201410798205.9 a kind of methods for disclosing water lily tissue cultures, specific steps are as follows:
1) preparation of culture medium, the component including minimal medium and each stage culture medium of tissue culture;2) selection and disinfection of explant;3)
The induction and proliferation of adventitious bud;4) culture of rootage;5) it transplants.
Although above-mentioned patent application is the breeding culture for water lily tissue, but need individually to prepare culture solution, needs to select
Fixed specific explant, and induction and proliferation to adventitious bud.But the application is without cultivating specific culture medium, using common
Soil, the application guarantee the suitable external temperature environment condition of water lily, and cultivating primarily directed to the control of temperature
Nurture body is handled before.Therefore, the breeding method of the application succinctly facilitates, and breeding method is easily realized, only needs
Controlling ambient temperature can be realized extensive cultivation, and can blade, fibrous root and rhizome to water lily cultivate, and on
Stating patent is only to cultivate rhizome.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of sides using water lily tissue rapid breeding
Method, the application can blade to water lily, fibrous root and rhizome carry out Propogation and culture, it is only necessary to control the external environment in cultivation period
Temperature, and activation processing is carried out to the water lily tissue of cultivation, it can be realized large-scale plantation cultivation.
The purpose of the present invention is achieved through the following technical solutions: a kind of side using water lily tissue rapid breeding
Method, the method that the blade including choosing water lily carries out breeding cultivation, specifically includes the following steps:
S1, the blade with base of leaf joint is chosen, blade surface is without necrosis, no disease and pests harm symptom;
S2, activation processing is carried out using base of leaf joint of the plant growth essence activity factor to blade surface, and will activation
Treated, and blade blade faces up, and is put on the water surface of Culture basin, and the water cover in Culture basin crosses earth surfaces, keeps culture
Environment temperature locating for basin is 20 DEG C -28 DEG C;
S3, after being cultivated 10-20 days in Culture basin, the base of leaf joint director of blade surface goes out tender shoots, continue cultivate 5-
After 10 days, when the tender shoots of blade grows 3-4 piece blade, blade lower section grows rhizome, and rhizome size 1-3cm is transplanted into plantation
Basin, 10-12 centimeters of nest line-spacing, keeping the ambient temperature of Pot for cultivating plant is 20 DEG C -28 DEG C, and blade declines Huang at this time, does not absorb
The function of nutrition.
In the present invention, the plant growth essence activity factor includes the plant nutrient factor, microelement, plant cell point
It splits element, plant growth regulator and plant and quickly absorbs element, microelement includes manganese element and Zn-ef ficiency.
In the present invention, in step s 2, when further including that tender shoots just grows, it is raw that plant is picked using 3 centimetres of loose cotton balls
Long essence activity factor solution wipes tender shoots, carries out activation growth and disinfection to tender shoots.
In the present invention, in step s 2, when further including that tender shoots just grows, using cotton swab pick the activation of plant growth essence because
Sub- solution wipes tender shoots, carries out moisturizing with loose wet cotton balls to tender shoots.
It in the present invention, further include the method chosen water lily fibrous root and carry out breeding, specifically includes the following steps:
Choose sturdy water lily fibrous root, the fibrous root extra fibrous root and the thick shape tip of a root to fracture when including plant division;
Activation processing is carried out to fibrous root surface using plant growth essence activity factor, and will activation treated fibrous root insertion
Under soil in Culture basin, upward, keep environment temperature locating for Culture basin is 20 DEG C -28 DEG C to the point for making fibrous root;
After cultivating 10-20 days in Culture basin, long budding above fibrous root is long above fibrous root after continuing culture 5-10 days
Four leaves out, the rhizome size below fibrous root is 1-3cm, can transplant the ambient temperature that Pot for cultivating plant is kept into Pot for cultivating plant
It is 20 DEG C -28 DEG C.
It in the present invention, further include the method chosen water lily rhizome and carry out breeding, specifically includes the following steps:
Rhizome is selected to have bud eye rhizome to be cut into block, block diameter is 5cm-8cm;The point for making radical bud block upward, is kept locating for Culture basin
Environment temperature be 20 DEG C -28 DEG C;
After being cultivated 10-20 days in Culture basin, long budding above rhizome, after continuing culture 5-10 days, above rhizome
Four leaves are grown, can transplant and keep the ambient temperature of Pot for cultivating plant into Pot for cultivating plant is 20 DEG C -28 DEG C, and the Pot for cultivating plant is
35 centimeters big 25 centimeters of depths insert sticky fertile soil in Pot for cultivating plant, and seeding row spacing is 15*15cm in Pot for cultivating plant.
It in the present invention, further include the method for water lily breeding seed dormancy preservation, method is to mix water lily breeding seed
Enter in a small amount of soil, water surface lid crosses earth surfaces, and ambient temperature range is 0 ° ± 5 DEG C.
The beneficial effects of the present invention are:
(1) it can bloom 30 for every plant, and unlimited bud can be formed, the seed of every plant of harvest is at 100 or more, germination percentage
60% or more;
(2) the step of Propogation and culture is few, and required cultivation material and cultivation drug are few, low in cost, is suitable for extensive
Propogation and culture;
(3) it is not necessarily to manage with delicacy during Propogation and culture, it is only necessary to control the ambient temperature condition of Propogation and culture, train
It is simple to educate process, breeding condition is easy to realize.
Detailed description of the invention
Fig. 1 is flow diagram of the invention.
Specific embodiment
Technical solution of the present invention is described in further detail with reference to the accompanying drawing, but protection scope of the present invention is not limited to
It is as described below.
Embodiment 1:
Applicant in African three plants of the water lily of acquisition in 2015, as shown in Figure 1, in early August, 2015 Sichuan Deyang in
Buy 10 plants in June, 2016 again, price is 45-60 member, after carrying out cleaning and sterilizing to blade, then has base of leaf to blade center place
Joint carries out wiping activation using plant growth essence activity factor, then covers the disinfectant analysis of a small amount of stimulating and keep gentle, and water is rigid
Good then blade face, Culture basin are put in 20 DEG C -28 DEG C of environment, and after 15 days, the place on blade with base of leaf joint is grown
Tender shoots, then cultivate 5 days, tender shoots gradually grows up to fibrous root, grows fibrous root and rhizome below prophyll piece.To December since late August.
Applicant using blade breeding method to kind ectogenesis repeatedly, in late November, 2016 in total Propogation and culture at blade
More than 280 strain of seedling.
Embodiment 2:
The thick shape fibrous root more than 10 that applicant has been broken after Sichuan Mianzhu's plant division in late August, 2015 saves, and fibrous root is cleaned
Completely, after disinfection treatment, wiping activation is carried out using plant growth essence activity factor, and the fibrous root after activation is embedded to Culture basin
Soil below, soil and water are housed in Culture basin, water just covered soil face, and after Culture basin is placed in outdoor 18 days,
Blade above fibrous root has grown bud-leaf, and after applicant transplants fibrous root, after five days, the fibrous root seedling 60% after transplanting was survived.
Embodiment 3:
Applicant wins 2 parts of rhizomes in Sichuan Chengdu in early March, 2016, and rhizome is cleaned up, and by two roots
Stem is cut into 15 fritters, and each fritter rhizome is embedded in Culture basin, and soil and water are housed in Culture basin, and water just covered mud
Native face, and Culture basin is placed in greenhouse, after the temperature in greenhouse is 23 DEG C, 16 days, it is found by the applicant that there is unusual fluctuation in soil face,
Applicant digs out fritter rhizome, and discovery fritter rhizome lower section has tender shoots to emit with fibrous root, fritter rhizome top with small green spot
Trace out.Rhizome is embedded in soil by applicant again, and emerging after 6 days, on soil has green point, and carefully identification has three pieces
Leaflet tablet, applicant transplant fritter rhizome into Pot for cultivating plant, and keeping the ambient temperature of Pot for cultivating plant is 25 DEG C constant, after 10 days,
15 root tuber stem nursery are all survived.
Embodiment 4:
The present embodiment is established on the basis of above-mentioned several embodiments, the plant water quality for selecting suitable water lily to cultivate, specifically
It see the table below:
Table 1: the water quality selection of water lily tissue cultivating
As seen from the above table, the effect of marsh gas water cultivation water lily is best, and therefore, rich water influences very the cultivation of water lily plantation
Greatly.Meanwhile the optional crops soil of soil property, black earth and the loess for cultivating water lily, but since the acquisition of black earth and loess is more inconvenient, i.e.,
Using traditional crops soil as soil is cultivated, growth conditions, which obtains, to be easy, low in cost, is cultivated convenient for large-scale breeding,
Wherein cultivating process band cohesive soil is best.
The application can blade to water lily, fibrous root and rhizome carry out Propogation and culture, be able to achieve mass propagation cultivation, and should
Breeding condition needed for method is easy, cultivates low in cost, it is only necessary to control the ambient temperature in cultivation period, reveal the 4-10 month
Its cultivation, needs to build sunshade 35 DEG C of 6-10 month or more, remaining time can be with greenhouse cultivation, as long as guaranteeing external environment temperature
Degree.The cultural method can be realized commercialization ectogenesis, can realize mass propagation in each timing node, especially
Year flower cultivation period can breed and provide a large amount of finished product for its demand, and ornamental, nursery, numerous can be played by cultivating lunar New Year flowers
Three good results not accidentally such as grow.
Further, the cultivation of water lily needs several external conditions, first is that needs are sunny, the environment of air circulation,
The environment excessively covered is unfavorable for the formation of bud, second is that should not plant in flowing water, torrent environment can rush the soil of plantation
Fall, the nutriment etc. for including in soil is flushed away;Third is that when blade is bred, blade must the water surface in succession, therefore, the water surface needs to compare mud
Soil is 5-20 centimeters or so high, and blade can absorb nutriment in the water surface;The water lily of the four 3-9 months outdoor cultivation does not need greenhouse, works as summer
The excessively high needs of temperature establish shade device, and water lily blade is avoided to be burnt;Fifth is that when the water lily temperature of potting is more than 35 DEG C, soil
Fibrous root can be burnt, therefore, must be transplanted during this time to shady place.
The water lily planted using method described herein is to have unlimited bud in the case where growing environment is suitable for
, that is, 20 DEG C -28 DEG C are maintained the temperature at, the external conditions such as bright and clear, fertile soil, the water lily of applicant has reached so far
More than 180 plants, and can normally bloom, result, production kind, it is best with blade breeding in above-mentioned several embodiments.
A specific embodiment of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (6)
1. a kind of method using water lily tissue rapid breeding, which is characterized in that the blade including choosing water lily carries out breeding training
The method educated, specifically includes the following steps:
S1, the blade with base of leaf joint is chosen, blade surface is without necrosis, no disease and pests harm symptom;
S2, activation processing is carried out using base of leaf joint of the plant growth essence to blade surface, and will activation treated blade
It faces up, is put on the water surface of Culture basin, the water cover in Culture basin crosses earth surfaces, keeps environment temperature locating for Culture basin
It is 20 DEG C -28 DEG C;
S3, after being cultivated 10-20 days in Culture basin, the base of leaf joint director of blade surface goes out tender shoots, continue culture 5-10 days
Afterwards, when the tender shoots of blade grows 3-4 piece blade, blade lower section grows rhizome, and rhizome size 1-3cm is transplanted into Pot for cultivating plant, nest
10-12 centimeters of line-spacing, keeping the ambient temperature of Pot for cultivating plant is 20 DEG C -28 DEG C.
2. a kind of method using water lily tissue rapid breeding according to claim 1, which is characterized in that the plant growth
Spermatophore includes plant growth regulator, manganese element and Zn-ef ficiency.
3. a kind of method using water lily tissue rapid breeding according to claim 1, which is characterized in that in step s 2,
Loose disinfectant cotton balls is stamped on base of leaf joint.
4. a kind of method using water lily tissue rapid breeding according to claim 1, which is characterized in that in step s 2,
When further including that tender shoots just grows, plant growth essence solution wiping tender shoots is picked using cotton swab, tender shoots is carried out with loose wet cotton balls
Moisturizing.
5. a kind of method using water lily tissue rapid breeding, which is characterized in that carry out the side of breeding including choosing water lily fibrous root
Method, specifically includes the following steps:
Choose sturdy water lily fibrous root, the fibrous root extra fibrous root and the thick shape tip of a root to fracture when including plant division;
Activation processing is carried out to fibrous root surface using plant growth essence, and by the mud in activation treated fibrous root insertion Culture basin
Under soil, upward, keep environment temperature locating for Culture basin is 20 DEG C -28 DEG C to the point for making fibrous root;
After cultivating 10-20 days in Culture basin, long budding above fibrous root after continuing culture 5-10 days, grows four above fibrous root
Piece leaf, the rhizome size below fibrous root are 1-3cm, and can transplant and keep the ambient temperature of Pot for cultivating plant into Pot for cultivating plant is 20
℃-28℃。
6. a kind of method using water lily tissue rapid breeding according to claim 1, which is characterized in that further include that water lily educates
The method that kind seed dormancy saves, method are that water lily breeding seed is mixed into a small amount of soil, and water surface lid crosses earth surfaces, and
Ambient temperature range is 0 DEG C ± 5 DEG C.
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