CN109156189A - A kind of grafting green cucumber root-planting method - Google Patents
A kind of grafting green cucumber root-planting method Download PDFInfo
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- CN109156189A CN109156189A CN201811127064.2A CN201811127064A CN109156189A CN 109156189 A CN109156189 A CN 109156189A CN 201811127064 A CN201811127064 A CN 201811127064A CN 109156189 A CN109156189 A CN 109156189A
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- grafting
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- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of grafting green cucumber root-planting methods, using with 2 true leaves, root system is complete, the cucumber seedling of in the same size, no disease and pests harm, robust growth is as grafting seedling, selecting one plant of cucumber seedling, beveling forms notch from top to bottom at its hypocotyl, another plant of cucumber seedling chamfers from bottom to top in the same location of hypocotyl and obtains notch, it is fixed after the docking of the two notch is chimeric with Grafting clip, stock true leaf and growing point are removed after grafting survival, there are two complete root system and a shared scion for the cucumber seedling tool after making grafting.Root-planting method of the invention is simple and easy, it is high-efficient, divide offspring high survival rate, ensure that root system integrality and two sides root system consistency, it can be used for carrying out the experimental studies such as the accurate subregion irrigation of the crops such as cucumber, root system stress and Nutrient Absorption, there is important scientific research value；It can also be used for the actual productions link such as culture of crop High efficiency and high quality cultivation and ornamental plant.
Description
Technical field
The present invention relates to a kind of root-planting methods of cucumber seedling to realize the method for dividing root specifically by the grafting of cucumber seedling.
Background technique
It is found in the research to fields such as all kinds of stress (such as moisture, saline and alkaline) and Nutrient Absorptions, it is native under natural conditions
Each factor (environmental factor, nutritive water equality) is all different in both horizontally and vertically distribution in earth, and this nonuniformity is to people
More acurrate meticulously understand plant in depth and bring certain difficulty.Blackman and Davies (1985) is to probe into soil moisture
Influence of the content to corn stomatal movement, takes the lead in designing and root-planting method is utilized.Then, root-planting method is in various crops
It is widely used in the research of (such as cotton), garden crop (such as cucumber, citrus).Using dividing the method for root that can not only simulate
The growth conditions of plant under field conditions (factors), and certain factor pair under the conditions of root system two sides are in identical or different can be probed into
The influence of the plant growth and development keeps research easier, quick and accurate, and can Enhancement test result accuracy and persuasion
Power.
Currently, the root-planting method generallyd use is directly to be divided into two parts root system or more parts.But due to root system of plant quantity
It is numerous, it is difficult to accomplish to divide equally.And for system of taproot plant, it is also necessary to first cut main root before dividing root, it is original to destroy root system
Structure, need specific culture apparatus carry out point offspring culture and survival rate it is low, the experimental study period is also grown, more unfavorable
It is the situation for causing research material that cannot react plant normal growth development completely due to cutting main root, greatly reduces research knot
The accuracy of fruit.Thus, this carrys out inconvenience for studying the system of taproot vegetational zone as cucumber.Dong Hezhong etc. (2010) is built
A kind of method for dividing root for cotton has been found, has divided root research to provide new way for cotton using a kind of grafting means.But due to
There are larger differences in growth and development characteristic and in the structure and composition of stem for cucumber and cotton, use its institute through test of many times
The method grafting green cucumber shoot survival percent of description is undesirable.Therefore, a kind of neither destruction main root is established, and can improve and divide root cucumber
The novel root-planting method of shoot survival percent is very necessary.
Summary of the invention
The technical problem to be solved by the present invention is to according to defect of the existing technology, provide one kind to be suitble to include that cucumber exists
The Split-Root Nutrient Solution Method of the simple and effective of interior system of taproot plant, high-survival rate.Two sides root system is complete, consistent after this method can guarantee point root
Good, the grafting root-planting shoot survival percent nearly 100% of property.
The technical scheme adopted by the invention is that:
A kind of grafting green cucumber root-planting method, using having, 2 true leaves, root system be complete, in the same size, no disease and pests harm, growth
Healthy and strong cucumber seedling is as grafting, and selecting one plant of cucumber seedling, beveling forms notch, another strain from top to bottom at its hypocotyl
Cucumber seedling chamfers from bottom to top in the same location of hypocotyl and obtains notch, is fixed after the docking of the two notch is chimeric with Grafting clip；
The cucumber seedling for forming notch from top to bottom is stock strain, and it is scion strain that another strain, which has the cucumber seedling of notch from bottom to top,
Stock true leaf and growing point are removed after grafting survival；Being divided to offspring tool, there are two complete root systems to contain two hypocotyls, and one shares
Scion.
Further, the method specifically comprises the following steps:
(1) full, neat and consistent cucumber seeds, seed soaking, vernalization 1. seed treatment: the preparation of cucumber seedling: are chosen；2. selecting
It takes the consistent seed that germinates to be seeded in the hole tray of the seedling medium equipped with sterilizing, after planting covers the seedling medium of one layer of sterilizing；
3. hole tray after planting is set in phjytotron, after seedling grows the 1st true leaf, appropriateness controls water and promotes root growth hair
It educates, nurturing staff；After seedling grows 2 true leaves, choose that root system is complete, cucumber of in the same size, no disease and pests harm, robust growth
Seedling, a part are used as scion strain, and a part is used as stock strain, and preparation starts to graft；
(2) it grafts and divides root: taking scion, be in 15~20 degree of angularly upper oblique partial applications at 1~1.5cm of its cotyledon lower part,
Depth reaches plumular axis diameter about 3/5~2/3；Stock is taken, is in 15~20 degree of angular lower bevelings at 1~1.5cm of its cotyledon lower part,
Depth reaches plumular axis diameter about 3/5~2/3；It is fixed after stock and the docking of the notch of scion are fitted into Grafting clip, after grafting survival
Remove the true leaf and growing point of stock.
Further, 1. seed treatment in step (1): full, neat and consistent cucumber seeds are chosen, 55 DEG C are hoted water treatment of seeds
10 minutes, then stirring to room temperature is soaked seed 6 hours, and seed is put in the culture dish for being covered with the double-deck filter paper with distillation water-soaked
It is interior, it is placed in 28 DEG C of insulating boxs vernalization 24 hours.
Further, 32 holes that the consistent seed that germinates is seeded in the seedling medium equipped with sterilizing are 2. chosen in step (1)
Hole tray irrigates bottom water before sowing, every hole broadcasts 1, depth about 2cm, after planting covers the seedling medium of about 1cm thickness sterilizing；Wherein,
Seedling medium is that 1:1 is mixed by volume for turf and vermiculite.
Further, 3. hole tray after planting to be set in phjytotron in step (1), diurnal temperature is 25 DEG C/15 DEG C,
Photoperiod 14 hours/10 hours, daytime 400 μm of olm of average light intensity-2s-1；After seedling grows the 1st true leaf, appropriateness
It controls water and promotes root growth and development, nurturing staff；After seedling grows 2 true leaves, choose root system it is complete, it is in the same size, without disease pest
The cucumber seedling of evil, robust growth, a part are used as scion strain, and a part is used as stock strain, and preparation starts to graft.
Further, grafting carries out in greenhouse in step (2), appropriate shading, and temperature is controlled at 20~25 DEG C；Graft work
Tool is sterilized with 70% medicinal alcohol.
Further, grafting is transplanted in the basin of bore 12cm high 10.5cm, is placed in artificial after grafting in step (2)
In climatic chamber, seedbed and plastic covering film are irrigated, appropriate shading treatment removes plastic film and shading facility after 2~3d, transfers
Connect the true leaf and growing point of rear 5~6d removal stock.
Beneficial effects of the present invention:
(1) the method for the present invention stock, scion during graft union is intact plant, and it is true to remove stock after healing
Leaf and growing point ensure that the consistency of two root growths in agglutination；It is original without directly destruction or indirect asynchronous stimulation
Two root systems ensure that two sides root system integrality and consistency, more accurately simulate cucumber normal growth situation.
(2) the method for the present invention is simple and easy, high-efficient；Divide root graft shoot survival percent high, nearly 100%；It is applied widely, it fits
The system of taproot plant preferably grafted can be used the method and divide root.
(3) the method for the present invention can be used for carrying out the accurate subregion irrigation of the crops such as cucumber, root system stress and Nutrient Absorption experiment
Deng research, there is important scientific research value；It is practical to can also be used for culture of crop High efficiency and high quality cultivation and ornamental plant etc.
Production link.
Detailed description of the invention
Fig. 1 is the schematic diagram of grafting green cucumber root-planting method.
Specific embodiment
The present invention is done below with reference to embodiment and attached drawing and is further explained.The following example is merely to illustrate this hair
It is bright, but be not used to limit practical range of the invention.
A kind of grafting green cucumber root-planting method, as shown in Figure 1,2 true leaves, root system is complete, in the same size, disease-free using having
Insect pest, robust growth cucumber seedling as grafting, select the beveling from top to bottom at its hypocotyl of one plant of cucumber seedling to be formed and cut
Mouthful, another plant of cucumber seedling chamfer from bottom to top in the same location of hypocotyl and obtain notch, the docking of the two notch be fitted into after with transferring
It is clamping fixed to connect；The cucumber seedling for forming notch from top to bottom is stock strain, and another strain has the cucumber seedling of notch from bottom to top
For scion strain, stock true leaf and growing point are removed after grafting survival；Being divided to offspring tool, there are two complete root systems to contain two hypocotyls,
One shared scion；
Specifically comprise the following steps:
(1) preparation of cucumber seedling:
1. seed treatment: full, neat and consistent cucumber seeds are chosen, 55 DEG C are hoted water treatment of seeds 10 minutes, stirring to room temperature,
Then it soaks seed 6 hours, seed is put in the culture dish for being covered with the double-deck filter paper with distillation water-soaked, is placed in 28 DEG C of insulating boxs
Vernalization 24 hours；
2. choosing the 32 hole hole trays that the consistent seed that germinates is seeded in the seedling medium equipped with sterilizing, pours and reveal the exact details before sowing
Water, every hole broadcast 1, depth about 2cm, after planting cover the seedling medium of about 1cm thickness sterilizing；Wherein, seedling medium be turf and
1:1 is mixed vermiculite by volume；
3. hole tray after planting is set in phjytotron, diurnal temperature is 25 DEG C/15 DEG C, and the photoperiod 14 hours/10 is small
When, daytime 400 μm of olm of average light intensity-2s-1；After seedling grows the 1st true leaf, appropriateness controls water and promotes root growth
Development, nurturing staff；After seedling grows 2 true leaves, choose that root system is complete, cucumber of in the same size, no disease and pests harm, robust growth
Seedling, a part are used as scion strain, and a part is used as stock strain, and preparation starts to graft；
(2) graft and divide root: grafting carries out in greenhouse, appropriate shading, and at 20~25 DEG C, grafting tool is used for temperature control
The disinfection of 70% medicinal alcohol；Scion is taken, angularly upper oblique partial application, depth reach in 15~20 degree at 1~1.5cm of its cotyledon lower part
To plumular axis diameter about 3/5~2/3；Stock is taken, in 15~20 degree of angular lower bevelings at 1~1.5cm of its cotyledon lower part, depth reaches
Plumular axis diameter about 3/5~2/3；It is fixed after stock and the docking of the notch of scion are fitted into Grafting clip, moves grafting after grafting
It in the basin for planting bore 12cm high 10.5cm, is placed in artificial climate room, irrigates seedbed and plastic covering film, at appropriate shading
It manages, plastic film and shading facility is removed after 2~3d, 5~6d removes the true leaf and growing point of stock after grafting.
Embodiment 1
Selection enriches full " excellent No. 1 of saliva " cucumber seeds and carries out vernalization processing: 55 DEG C are hoted water treatment of seeds 10 minutes, and stirring is extremely
Then room temperature is soaked seed 6 hours, seed is put in the culture dish for being covered with the double-deck filter paper with distillation water-soaked, is placed in 28 DEG C of perseverances
Vernalization seed is made in vernalization 24 hours in incubator；32 holes of the seedling medium equipped with sterilizing can be seeded in after seed shows money or valuables one carries unintentionally
Seedling culture hole plate irrigates bottom water before sowing, every hole broadcasts 1, depth about 2cm, after planting covers the nursery base of the thick sterilizing of 1cm or so
Matter, seedling medium are that 1:1 is mixed by volume for turf and vermiculite；Hole tray after planting is set in phjytotron, diurnal temperature
Be 25 DEG C/15 DEG C, the photoperiod 14 hours/10 hours, daytime 400 μm of olm of average light intensity-2s-1；The 1st is grown to seedling
After piece true leaf, appropriateness controls water and promotes root growth and development, nurturing staff；After seedling grows 2 true leaves, it is complete, big to choose root system
Small consistent, no disease and pests harm, robust growth cucumber seedling, a part are used as scion strain, and a part is used as stock strain, and preparation starts to transfer
It connects；Grafting carries out in greenhouse, appropriate shading, and temperature is controlled at 20~25 DEG C, and grafting tool is sterilized with 70% medicinal alcohol；It takes
Scion, in 15~20 degree, angularly upper oblique partial application, depth reach plumular axis diameter about 3/5~2/ at 1~1.5cm of its cotyledon lower part
3；Stock is taken, in 15~20 degree of angular lower bevelings at 1~1.5cm of its cotyledon lower part, depth reaches plumular axis diameter about 3/5~2/3,
Institute's cut-grafting fringe should be consistent as far as possible with stock joint surface area, clamping with grafting after stock and the docking of the notch of scion are fitted into
It is fixed；Grafting is transplanted in the seedling-growing container of bore 12cm high 10.5cm after grafting, is placed in artificial climate room, irrigates seedbed simultaneously
Plastic covering film, appropriate shading treatment remove plastic film and shading facility after 2~3d, and 5~6d removes stock after grafting
True leaf and growing point；Grafting after healing is carefully taken out from matrix, clear water washes root, removes matrix on root, gained grafting
Divide offspring two sides root system complete, consistent, grafting root-planting shoot survival percent 100%.
Embodiment 2
Selection enriches full " the close thorn in Xintai City " cucumber seeds and carries out vernalization processing: 55 DEG C are hoted water treatment of seeds 10 minutes, stirring
It to room temperature, then soaks seed 6 hours, seed is put in the culture dish for being covered with the double-deck filter paper with distillation water-soaked, is placed in 28 DEG C
Vernalization seed is made in vernalization 24 hours in insulating box；32 of the seedling medium equipped with sterilizing can be seeded in after seed shows money or valuables one carries unintentionally
Hole seedling culture hole plate irrigates bottom water before sowing, every hole broadcasts 1, depth about 2cm, after planting covers the nursery base of the thick sterilizing of 1cm or so
Matter, seedling medium are that 1:1 is mixed by volume for turf and vermiculite；Hole tray after planting is set in phjytotron, diurnal temperature
Be 25 DEG C/15 DEG C, the photoperiod 14 hours/10 hours, daytime 400 μm of olm of average light intensity-2s-1；The 1st is grown to seedling
After piece true leaf, appropriateness controls water and promotes root growth and development, nurturing staff；After seedling grows 2 true leaves, it is complete, big to choose root system
Small consistent, no disease and pests harm, robust growth cucumber seedling, a part are used as scion strain, and a part is used as stock strain, and preparation starts to transfer
It connects；Grafting carries out in greenhouse, appropriate shading, and temperature is controlled at 20~25 DEG C, and grafting tool is sterilized with 70% medicinal alcohol；It takes
Scion, in 15~20 degree, angularly upper oblique partial application, depth reach plumular axis diameter about 3/5~2/ at 1~1.5cm of its cotyledon lower part
3；Stock is taken, in 15~20 degree of angular lower bevelings at 1~1.5cm of its cotyledon lower part, depth reaches plumular axis diameter about 3/5~2/3,
Institute's cut-grafting fringe should be consistent as far as possible with stock joint surface area, clamping with grafting after stock and the docking of the notch of scion are fitted into
It is fixed；Grafting is transplanted in the seedling-growing container of bore 12cm high 10.5cm after grafting, is placed in artificial climate room, irrigates seedbed simultaneously
Plastic covering film, appropriate shading treatment remove plastic film and shading facility after 2~3d, and 5~6d removes stock after grafting
True leaf and growing point；Grafting after healing is carefully taken out from matrix, clear water washes root, removes matrix on root, gained grafting
Divide offspring two sides root system complete, consistent, grafting root-planting shoot survival percent 100%.
Claims (7)
1. a kind of grafting green cucumber root-planting method, which is characterized in that using having, 2 true leaves, root system is complete, in the same size, disease-free
Insect pest, robust growth cucumber seedling as grafting, select the beveling from top to bottom at its hypocotyl of one plant of cucumber seedling to be formed and cut
Mouthful, another plant of cucumber seedling chamfer from bottom to top in the same location of hypocotyl and obtain notch, the docking of the two notch be fitted into after with transferring
It is clamping fixed to connect；The cucumber seedling for forming notch from top to bottom is stock strain, and another strain has the cucumber seedling of notch from bottom to top
For scion strain, stock true leaf and growing point are removed after grafting survival；Being divided to offspring tool, there are two complete root systems to contain two hypocotyls,
One shared scion.
2. grafting green cucumber root-planting method according to claim 1, which is characterized in that the method specifically includes following step
It is rapid:
(1) full, neat and consistent cucumber seeds, seed soaking, vernalization 1. seed treatment: the preparation of cucumber seedling: are chosen；2. choosing hair
The consistent seed of bud is seeded in the hole tray of the seedling medium equipped with sterilizing, after planting covers the seedling medium of one layer of sterilizing；3. will
Hole tray after planting is set in phjytotron, and after seedling grows the 1st true leaf, appropriateness controls water and promotes root growth and development, training
Educate strong sprout；After seedling grows 2 true leaves, choose that root system is complete, cucumber seedling of in the same size, no disease and pests harm, robust growth, one
It is allocated as scion strain, a part is used as stock strain, and preparation starts to graft；
(2) it grafts and divides root: taking scion, in 15~20 degree of angularly upper oblique partial applications, depth at 1~1.5cm of its cotyledon lower part
Reach plumular axis diameter about 3/5~2/3；Stock is taken, in 15~20 degree of angular lower bevelings, depth at 1~1.5cm of its cotyledon lower part
Up to plumular axis diameter about 3/5~2/3；It is fixed after stock and the docking of the notch of scion are fitted into Grafting clip, is removed after grafting survival
The true leaf and growing point of stock.
3. grafting green cucumber root-planting method according to claim 2, which is characterized in that 1. seed treatment in step (1): choosing
Full, neat and consistent cucumber seeds, 55 DEG C are hoted water treatment of seeds 10 minutes, then stirring to room temperature soaks seed 6 hours, seed is put
In in the culture dish for being covered with the double-deck filter paper with distillation water-soaked, it is placed in 28 DEG C of insulating boxs vernalization 24 hours.
4. grafting green cucumber root-planting method according to claim 2, which is characterized in that it is consistent 2. to choose germination in step (1)
Seed be seeded in equipped with sterilizing seedling medium 32 hole hole trays, bottom water is irrigated before sowing, every hole broadcasts 1, depth about 2cm,
After planting cover the seedling medium of about 1cm thickness sterilizing；Wherein, seedling medium is that 1:1 is mixed by volume for turf and vermiculite.
5. grafting green cucumber root-planting method according to claim 2, which is characterized in that 3. by cave after planting in step (1)
Disk is set in phjytotron, and diurnal temperature is 25 DEG C/15 DEG C, the photoperiod 14 hours/10 hours, daytime 400 μ of average light intensity
mol·m-2s-1；After seedling grows the 1st true leaf, appropriateness controls water and promotes root growth and development, nurturing staff；Seedling grows 2
After piece true leaf, choose that root system is complete, cucumber seedling of in the same size, no disease and pests harm, robust growth, a part is used as scion strain, and one
Part is used as stock strain, and preparation starts to graft.
6. grafting green cucumber root-planting method according to claim 2, which is characterized in that in step (2) grafting in greenhouse into
Row, appropriate shading, temperature are controlled at 20~25 DEG C；Grafting tool is sterilized with 70% medicinal alcohol.
7. grafting green cucumber root-planting method according to claim 2, which is characterized in that by grafting after grafting in step (2)
It is transplanted in the basin of bore 12cm high 10.5cm, is placed in artificial climate room, irrigate seedbed and plastic covering film, appropriate shading
It handles, plastic film and shading facility is removed after 2~3d, 5~6d removes the true leaf and growing point of stock after grafting.
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| CN201811127064.2A CN109156189A (en) | 2018-09-27 | 2018-09-27 | A kind of grafting green cucumber root-planting method |
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| CN201811127064.2A CN109156189A (en) | 2018-09-27 | 2018-09-27 | A kind of grafting green cucumber root-planting method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754304A (en) * | 2019-11-16 | 2020-02-07 | 福建农林大学 | Method for improving cucumber grafted seedling healing growth and seedling quality by utilizing light quality combination |
| CN111699852A (en) * | 2020-06-05 | 2020-09-25 | 五峰雅禾农业发展有限公司 | Cucumber grafting seedling method |
| CN112088671A (en) * | 2020-10-20 | 2020-12-18 | 莫翌 | Method for cultivating multiple seedlings by using grafting technology |
| CN112753499A (en) * | 2020-12-30 | 2021-05-07 | 四川省农业科学院经济作物育种栽培研究所 | Tomato multiple root system seedling raising method |
| CN115250773A (en) * | 2022-07-06 | 2022-11-01 | 建水县浩野农林产业有限公司 | Rapid seedling raising technology for grafting multiple root system cutting seedlings and seeding seedlings of camellia oleifera trees |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754304A (en) * | 2019-11-16 | 2020-02-07 | 福建农林大学 | Method for improving cucumber grafted seedling healing growth and seedling quality by utilizing light quality combination |
| CN111699852A (en) * | 2020-06-05 | 2020-09-25 | 五峰雅禾农业发展有限公司 | Cucumber grafting seedling method |
| CN112088671A (en) * | 2020-10-20 | 2020-12-18 | 莫翌 | Method for cultivating multiple seedlings by using grafting technology |
| CN112753499A (en) * | 2020-12-30 | 2021-05-07 | 四川省农业科学院经济作物育种栽培研究所 | Tomato multiple root system seedling raising method |
| CN115250773A (en) * | 2022-07-06 | 2022-11-01 | 建水县浩野农林产业有限公司 | Rapid seedling raising technology for grafting multiple root system cutting seedlings and seeding seedlings of camellia oleifera trees |
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Application publication date: 20190108 |