CN104855259B - Sinapsis alba seedling water planting method - Google Patents
Sinapsis alba seedling water planting method Download PDFInfo
- Publication number
- CN104855259B CN104855259B CN201510214920.8A CN201510214920A CN104855259B CN 104855259 B CN104855259 B CN 104855259B CN 201510214920 A CN201510214920 A CN 201510214920A CN 104855259 B CN104855259 B CN 104855259B
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- dilution
- sinapsis alba
- seedling
- nutrient solutions
- culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a sinapsis alba seedling water planting method which comprises the following steps: accelerating germination of sinapsis alba seedling seeds subjected to jarovization, placing seeds with radicles in a culture plate, placing the culture plate in a culture pot, adding water in the culture pot to enable the root parts of the radicles to be in contact with the water surface, placing the culture pot in a culture chamber for cultivation for 7-8 days so as to obtain a plantlet with two leaves and one heart, replacing water in the pot with a diluent Hoagland's nutrient solution, placing a KT plate in the pot, enabling the root parts of the seedlings to be soaked in the diluent Hoagland's nutrient solution, and replacing the diluent Hoagland's nutrient solution once every five days. The sinapsis alba seedling water planting method is simple to operate, low in cost, and high in survival rate.
Description
Technical field
The present invention relates to plant cultivation field, more particularly to a kind of ciltivating process of sinapsis alba seedling.
Background technology
Sinapsis alba（Sinapis alba L）It is a kind of spring habit oil crops of Cruciferae sinapsis alba category, is also a kind of medicinal plant
Thing, sinapsis alba has more excellent economical character, utilizes it as excellent genes source and carries out the research of plant hybridization breeding and exists
Countries in the world carry out more.Carry out botany research using sinapsis alba, can be metabolism way under various environment stresses in excavation plant body
The information in footpath, find and clone important adversity gene to provide basic test material.At present, the training method of sinapsis alba mainly has turf
Soil, vermiculite and perlitic mixotrophism medium and Solution culture method two ways.Wherein, water planting is the common one kind in laboratory
Planting type;But both at home and abroad the ciltivating process of report is cumbersome, relatively costly.
The content of the invention
It is an object of the invention to provide a kind of ciltivating process of sinapsis alba seedling, the simple low cost of method of operating, survival rate are high.
In order to solve above-mentioned technical problem, what the ciltivating process of the sinapsis alba seedling that the present invention is provided was realized in：
A kind of ciltivating process of sinapsis alba seedling, including：The sinapsis alba seed muonic catalysis of vernalization treatment is sprouted, radicle will be grown
Seed is placed on culture plate and is placed in Culture basin, adds water in Culture basin, the seed base-root root contact water surface, is placed on culturing room
In, cultivate 7 ~ 8 days, obtain two leaves wholeheartedly seedling；Then water in basin changed the Huo Gelan nutrient solutions of dilution into, KT plates are placed on basin
In, the root of seedling is immersed in the Huo Gelan nutrient solutions of dilution, and the Huo Gelan nutrient solutions of the dilution were changed once per 5 days,
Cultivate 9 ~ 10 days, sinapsis alba seedling is emerged when growing to 4 leaves wholeheartedly.
Optionally, has gauze on the culture plate.
Optionally, the radicle of the seed is inserted in the mesh of the gauze.
Optionally, the temperature of the culturing room be 24 DEG C -28 DEG C, humidity be 60%-80%, illumination is 6000lux, during illumination
Between be 16h/ days.
Optionally, when water in basin to be changed into the Huo Gelan nutrient solutions of dilution, the multiple of the Huo Gelan nutrient solutions dilution is 3
Times；When changing the Huo Gelan nutrient solutions of dilution for the first time, the multiple of the Huo Gelan nutrient solutions dilution is 2 times；Second and the
When the Huo Gelan nutrient solutions of dilution are changed after secondary, the multiple of the Huo Gelan nutrient solutions dilution is 1 times.
Optionally, the culture plate is：Uniformly it is covered with the KT plates that a diameter of 0.5 ~ 1.5cm, spacing are 0.5 ~ 1.5.
Optionally, the vernalization treatment is to place seed 14 ~ 16 days at 4 DEG C.Beneficial effects of the present invention：
The ciltivating process of sinapsis alba seedling, culture plate is positioned in rectangle cooking basin, it is adaptable to high-volume seedling growth test, and
Be conducive to the humidity for keeping suitable sinapsis alba seedling seedling to survive.The culture plate energy Reusability being cut into using KT plates, therefore can drop
Low production cost;It is cheap and KT plates are common consumptive material in daily life.Sinapsis alba seedling seedling is placed on and has been covered with medical aseptic
Cultivate on the KT plates of gauze, it is easy to operate and be easily handled;In the present invention, whole operation flow process is extremely simple easy.Transplant seedlings
The non-directly contact hand of overall process, thus it is improper also to produce manual operation, is moved using sponge wrapping relative in prior art
The death of seedling that cultivation causes, as long as the seedling that the sinapsis alba seedling ciltivating process that the present invention is provided can normally germinate inside culture dish,
In the present inventive method cultivation can survive, thus greatly improve survival rate.
Description of the drawings
Fig. 1 is that the sinapsis alba seedling ciltivating process that the present invention is provided cultivates two leaves for obtaining wholeheartedly seedling in water；
Fig. 2 is that the sinapsis alba seedling ciltivating process that the present invention is provided cultivates the sinapsis alba seedling for obtaining for 2 weeks in nutrient solution.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, following examples are carried out to the present invention
Further describe.It should be appreciated that specific embodiment described herein is not used to limit only to explain the present invention
The present invention.
The ciltivating process of the sinapsis alba seedling that the present invention is provided, comprises the steps：
1), the sprouting of seed:
Sterilized the min of sinapsis alba seed 3 Jing after vernalization treatment with 0.1% mercury chloride, afterwards with aseptic water washing 3 ~ 5 times, with
Uniformly it is positioned over afterwards and is covered with the culture dish of individual layer filter paper and gauze, adds appropriate distillation Aquaponic, the submergence seed of distilled water half.
After being disposed, in the indoor placement vernalization of culture 2 ~ 3 days, when root is about 0.5cm ~ 1 cm, can be used to plant.Culture bar
Part is 24 DEG C -28 DEG C of temperature, and it is 60%-80% to be humidified to degree with room humidifier.Vernalization is specifically：Seed is placed at 4 DEG C
14 ~ 16 days are stood,
2), culture plate is made:
Transformed from the KT plates of bulk, it is specific as follows so as to make required culture plate 1:
The KT plates of bulk are cut into the rectangle of 30cm × 20cm sizes with scissors;And onboard beaten using card punch
Go out aperture and pitch of holes is the aperture of 1cm, frame reserves 2cm width, after having openning hole, takes medical sterile gauze and is cut into 30cm
× 20cm sizes, are layered on KT plates, after gauze water breakthrough on i.e. tight attached and KT plates.KT plates are that one kind is generated by PS particles through foaming
Plate core, through a kind of new material of surface coating pressing, plate body is well-pressed, slim and graceful, be unlikely to deteriorate, easy to process, market
It is upper on sale.
From rectangle box is arranged as Culture basin, the long 38cm of rectangle culture box 2, width 26cm, high 11.5cm.
Each KT plate transformed is lengthened successively in square culture box.Running water, liquid level are added toward rectangle culture box
Highly it is 5cm, KT plates float on guarantee step 3 on liquid level as liquid level is raised）Transplanting to plate the root of seed has sufficiently
Growing space.
It should be noted that：Step 1）With 2）In no particular order sequentially, those skilled in the art can rationally pacify as needed
Row's time carries out seed and sprouts step and make culture plate.
3), transplant seedlings:
Indoor in culture, with tip tweezers by step 1) obtained by culture dish in the seed of vernalization gently press from both sides out, be put into
Step 2) obtained by culture plate on, the root for making seed is sufficiently submerged in nutrient solution liquid level through medical sterile gauze mesh.
In each aperture, transplant seedlings 1 ~ 2 plant.
Transplant seedlings after finishing, Culture basin is positioned over into cultivation in culturing room and obtains within 7 ~ 8 days or so two leaves wholeheartedly seedling.Culture is indoor
Temperature is 24 DEG C ~ 28 DEG C, humidity is 60%-80%, and using the bio-effect lamp being made up of white light, illumination value is 6000lux to light source,
Setting daylight hours are 16h, and plant is apart from the cm of light source 25 ~ 30.
4), nursery:
The culture plate swum on Culture basin liquid level is gently picked up and is vacantly positioned on one side, outwell the remnants in Culture basin
Running water simultaneously cleans up Culture basin, plus the Huo Gelan nutrient solutions of dilution, and the Huo Gelan nutrient solutions of dilution are：Huo Gelan nutrition
Liquid is with water with 1:3 volume mixture is uniform, then culture plate is gently put back to continuation culture in Culture basin.Changed per 5 days once dilute
The Huo Gelan nutrient solutions released.When changing the Huo Gelan nutrient solutions of dilution for the first time, the Huo Gelan nutrient solutions of dilution are：Huo Gelan seeks
Nutrient solution is with water with 1:2 volume mixture is uniform.When changing the Huo Gelan nutrient solutions of dilution second, the Huo Gelan nutrient solutions of dilution
It is：Huo Gelan nutrient solutions are with water with 1:1 volume mixture is uniform, and the Huo Gelan nutrient solutions of dilution, Huo Gelan nutrition are changed later
The extension rate of liquid is all 1 times.
Seedling raising process is carried out in culture interior, and culture indoor temperature is 24 DEG C ~ 28 DEG C, and humidity is 60% ~ 80%.Light source is adopted
The bio-effect lamp being made up of white light, illumination value is 6000lux, and setting light application time is daytime, daily 16h, and illumination value is
6000lux, plant is apart from 25 ~ 30cm of light source.Cultivate 9 ~ 10 days, cultivate when sinapsis alba seedling grows to 4 leaves wholeheartedly and complete.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (4)
1. a kind of ciltivating process of sinapsis alba seedling, it is characterised in that include：The sinapsis alba seed muonic catalysis of vernalization treatment is sprouted, will
The seed for growing radicle is placed on KT plates and is placed in Culture basin, adds water in Culture basin, the seed base-root root contact water surface, places
In culturing room, cultivate 7 ~ 8 days, obtain two leaves wholeheartedly seedling；Then water in basin changed into the Huo Gelan nutrient solutions of dilution, KT plates
In being placed on basin, the root of seedling is immersed in the Huo Gelan nutrient solutions of dilution, the Huo Gelan nutrient solutions of the dilution per 5 days more
Change once, cultivate 9 ~ 10 days, sinapsis alba seedling is emerged when growing to 4 leaves wholeheartedly；Uniformly it is covered with aperture on the KT plates, in the KT
Has gauze on plate, the radicle of seed is inserted in the mesh of the gauze.
2. the ciltivating process of sinapsis alba seedling according to claim 1, it is characterised in that the temperature of the culturing room is 24 DEG C-
28 DEG C, humidity be 60%-80%, illumination is 6000lux, and light application time is 16h/ days.
3. the ciltivating process of sinapsis alba seedling according to claim 1 and 2, it is characterised in that change water in basin into dilution suddenly
During Glan nutrient solution, the multiple of the Huo Gelan nutrient solutions dilution is 3 times；When changing the Huo Gelan nutrient solutions of dilution for the first time,
The multiple of the Huo Gelan nutrient solutions dilution is 2 times；When changing the Huo Gelan nutrient solutions of dilution after second and second,
The multiple of the Huo Gelan nutrient solutions dilution is 1 times.
4. the ciltivating process of sinapsis alba seedling according to claim 3, it is characterised in that the vernalization treatment is 4 by seed
DEG C place 14 ~ 16 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510214920.8A CN104855259B (en) | 2015-04-30 | 2015-04-30 | Sinapsis alba seedling water planting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510214920.8A CN104855259B (en) | 2015-04-30 | 2015-04-30 | Sinapsis alba seedling water planting method |
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| CN104855259A CN104855259A (en) | 2015-08-26 |
| CN104855259B true CN104855259B (en) | 2017-05-10 |
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| CN201510214920.8A Expired - Fee Related CN104855259B (en) | 2015-04-30 | 2015-04-30 | Sinapsis alba seedling water planting method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165581B (en) * | 2015-09-23 | 2018-06-19 | 江西省科学院生物资源研究所 | A kind of ciltivating process of capsicum |
| CN107593410A (en) * | 2017-11-03 | 2018-01-19 | 中国农业科学院特产研究所 | A kind of ciltivating process of ginseng-leaf |
| CN111149466B (en) * | 2020-04-01 | 2024-06-18 | 浙江美之奥种业股份有限公司 | Melon seed water seedling cultivation method and device |
| CN115500252B (en) * | 2022-08-31 | 2024-02-27 | 郑州大学 | Hydroponic method for rapid rooting of reed leaves and reeds |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100515175C (en) * | 2006-08-02 | 2009-07-22 | 中国科学院植物研究所 | Method for cultivation of arabidopsis |
| CN101142893B (en) * | 2007-10-09 | 2010-11-17 | 浙江大学 | Water-planting method for arabidopsis thaliana |
| CN101731135B (en) * | 2008-11-26 | 2011-08-24 | 中国科学院大连化学物理研究所 | Device for directly seeding and culturing arabidopsis thaliana in water and application thereof |
| CN102265780A (en) * | 2011-05-15 | 2011-12-07 | 浙江大学 | Simple seedling raising method of Arabidopsis seeds and used device |
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Granted publication date: 20170510 Termination date: 20180430 |