CN115500252B - Hydroponic method for rapid rooting of reed leaves and reeds - Google Patents
Hydroponic method for rapid rooting of reed leaves and reeds Download PDFInfo
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- CN115500252B CN115500252B CN202211059752.6A CN202211059752A CN115500252B CN 115500252 B CN115500252 B CN 115500252B CN 202211059752 A CN202211059752 A CN 202211059752A CN 115500252 B CN115500252 B CN 115500252B
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- 235000014676 Phragmites communis Nutrition 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 30
- 244000089486 Phragmites australis subsp australis Species 0.000 title 1
- 239000000843 powder Substances 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000003337 fertilizer Substances 0.000 claims abstract description 32
- 150000001875 compounds Chemical class 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 5
- 244000081757 Phalaris arundinacea Species 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 5
- 238000005520 cutting process Methods 0.000 description 13
- 244000025254 Cannabis sativa Species 0.000 description 8
- 241000287219 Serinus canaria Species 0.000 description 4
- 241001494508 Arundo donax Species 0.000 description 3
- 241000011500 Phyllostachys praecox Species 0.000 description 3
- 235000008545 Phyllostachys praecox Nutrition 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 244000273256 Phragmites communis Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241001494510 Arundo Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a water planting method for rapid rooting of reed rhizome. Firstly preparing an ABT rooting powder solution by using a rooting powder medicament, and then placing the reed rhizome nodes in the prepared rooting powder solution for culture treatment; preparing a compound fertilizer solution by using the compound fertilizer, and transferring the reed rhizome node treated by the rooting powder solution into the prepared compound fertilizer solution for culturing until rooting. The technical scheme of the invention has the advantages of simple and easy operation, lower requirements on production equipment, fewer working procedures and lower seedling raising cost, but can still obtain higher rooting rate in shorter time and has more roots.
Description
1. Technical field:
the invention relates to the technical field of seedling raising of reed canary grass, in particular to a water planting method for rapidly rooting of reed canary grass (fat grass).
2. The background technology is as follows:
the common reed, commercial name fat grass, belongs to Gramineae Arundo, the reed subtype is a multifunctional plant, and can be used as feed for herbivores, culture medium for edible (medicinal) fungus cultivation, paper production, plate production, biological energy production and wind prevention, sand fixation and soil restoration. Along with the gradual development of the functions of the reed grass (fat grass), the seedling demand of the reed grass is larger and larger. How to quickly propagate a large number of seedlings becomes a key problem for the application of the seedlings.
Since the reed canary cannot be bred through seeds, only asexual propagation is possible. At present, the propagation modes of the reed canary grass mainly comprise plant division, cuttage and tissue culture. The planting method is characterized in that 4-5 buds are used as a cluster for planting after the root-shaped stems are segmented, the survival rate is high, but the breeding speed is low, the seedlings are easy to carry toxicity, the labor intensity is excessive, and the cost is high. By utilizing a tissue culture mode, a large number of non-toxic seedlings with consistent growth can be obtained in a short time, but the method has higher requirements on production equipment and operation technology and has higher propagation cost. The cutting propagation is to use the overground stem with axillary buds as cutting slips for cutting seedling, and compared with the separation and tissue culture, the method is simple and convenient to operate and low in cost, and the overground stem of the reed canary is convenient to transport, so that the method is a main propagation mode of the reed canary at present.
At present, there are two main modes of cutting and raising the seedlings of arundo donax: one is to insert cutting slips into soil (matrix) for seedling cultivation; one is to put the cutting slips into water (culture solution) for rooting and seedling culture. Compared with the two methods, the rooting rate of the water planting method is higher, and the rooting time is shorter. For example: the invention patent application of CN111374033A discloses a method for raising seedlings of arundo donax by water culture cutting. Culturing the cutting slips in an aqueous culture solution until rooting to obtain rooted cutting slips; transplanting the rooted cutting, and performing conventional management to obtain the arundo donax seedlings. Wherein, the formula of the water culture solution is as follows: 1/5 Hoagland nutrient solution and NAA or IBA 0.14-0.16 mg/L. According to the method disclosed by the patent application, the statistical rooting rate reaches 90% from the beginning of water culture to the 28 th day. Although the method disclosed by the invention has higher rooting rate and shorter seedling period compared with the traditional soil culture cutting, the time for rooting is still longer, and further shortening the rooting time is of great significance for rapid propagation of the reed canary seedlings.
3. The invention comprises the following steps:
the invention aims to solve the technical problems that: aiming at the problem of long rooting time in the current water culture cutting of the reed canary grass (the fertilizer grass), the invention provides a water culture rooting method of the reed canary grass (the fertilizer grass) with short rooting time and high rooting rate, namely the water culture method for rapidly rooting the reed canary grass (the fertilizer grass).
In order to solve the problems, the invention adopts the following technical scheme:
the invention provides a water planting method for rapid rooting of reed rhizome (fat grass), which comprises the following steps:
a. preparing rooting powder solution: weighing 90-110 mg of rooting powder medicament, adding the rooting powder medicament into a beaker, adding 50mL of 95% ethanol for dissolution, and adding 950mL of water after dissolution to prepare 90-110 mg/L of ABT rooting powder solution;
b. rooting powder solution treatment: c, putting the reed rhizome nodes into the rooting powder solution prepared in the step A for treatment, wherein in the treatment process, the temperature is controlled to be 25+/-2 ℃, the illumination time is 16-17 h, the illumination intensity is 19000-20000 lx, the treatment time is 6 days, and the rooting powder solution is replaced every 3 days;
c. preparing a compound fertilizer solution: 1000mL of water is measured, 0.2-0.3 g of compound fertilizer is added, and the mixture is stirred until the mixture is completely dissolved, so as to obtain compound fertilizer solution with the concentration of 0.2-0.3 g/L;
d. and (3) solution culture of compound fertilizer: and c, transferring the reed rhizome node treated in the step b into a prepared compound fertilizer solution for culturing until rooting.
According to the rapid rooting water planting method for the reed canary grass, the rooting powder in the step a is No. 1 rooting powder.
According to the rapid rooting water planting method of the reed canary grass, in the step b, the reed canary grass stem nodes are main reed canary grass stems with axillary buds as culture nodes, each reed canary grass stem node is provided with one axillary bud, the length of the lower node is 2-4 cm, and the length of the upper node is 5-7 cm.
According to the rapid rooting water planting method of the reed canary grass, the compound fertilizer in the step c is a macroelement water-soluble fertilizer applied to kefeng.
According to the rapid rooting water planting method of the reed canary grass, the conditions in the culturing in the step d are as follows: the temperature is 25+/-2 ℃, the illumination time is 16-17 h, the illumination intensity is 19000-20000 lx, and the compound fertilizer solution is replaced every 3 days.
According to the water planting method for rapidly rooting the reed rhizome, the rooting powder solution in the step b and the compound fertilizer solution in the step d are treated, and the rooting powder solution or the compound fertilizer solution is 1-2 cm higher than the axillary bud node of the reed rhizome node (preferably, the reed rhizome node with a bevel under the node and a flat surface on the node).
The invention has the positive beneficial effects that:
1. the method solves the problem of long rooting time of the reed rhizome in water culture cuttage in the prior art by placing the reed rhizome node in 100mg/L of ABT No. 1 rooting powder solution for 6 days and then transferring the reed rhizome node into 0.25g/L of compound fertilizer solution for cultivation.
2. According to the technical scheme Shui Peijian phyllostachys praecox has the characteristics of rapid rooting and high rooting rate (in the preferred embodiment, the rooting rate can reach 90% from the beginning of water culture to the 16 th day, the statistical rooting rate reaches 96% from the 20 th day, and the average length of each phyllostachys praecox stem node is 9.15, and the average length of each phyllostachys praecox stem node is 6.04 cm).
3. Compared with the existing method for separating and tissue culturing the reed leaves, the technical scheme of the invention has the advantages of simple and easy operation, lower requirements on production equipment, fewer working procedures, lower seedling raising cost, higher rooting rate still obtained in a shorter time and more roots.
4. Description of the drawings:
FIG. 1 is a photograph of a reed rhizome of Sword leaf on day 20 of the water culture in the example of the present invention;
FIG. 2 is a photograph of a root system of a reed rhizome of Sword leaf for Shui Peidi days in the treatment group according to the embodiment of the present invention;
FIG. 3 is a photograph of a root system of a reed rhizome of Sword leaf for Shui Peidi days in a control group according to an embodiment of the present invention;
FIG. 4 is a statistical chart of rooting rate of hydroponic culture on different days according to the embodiment of the invention;
FIG. 5 is a graph showing root numbers and root length statistics on day 20 of hydroponics in an embodiment of the invention.
5. The specific embodiment is as follows:
the present invention will be further described in detail with reference to specific examples, to better understand the content of the present invention, but the technical solution of the present invention is not limited to the content defined in the following examples.
Example 1:
the invention relates to a water culture method for rapid rooting of reed grass (fat grass), which comprises the following steps:
a. preparing the reed rhizome node: selecting strong, disease-free and consistent-growing reed rhizome as a material in 2 months of 2022, cutting off the stem node with axillary buds which do not grow into branches as reed rhizome node, requiring each reed rhizome node to have an axillary bud, and simultaneously making the lower part of the node grow to about 3cm and the incision to be beveled; the length of the node is about 6cm, the incision is cut flatly, the leaves are removed, the axillary buds are not damaged, and the reed rhizome node obtained by the treatment is used as a water culture material;
b. treating the pedicel knots of the reed leaves: c, cleaning the reed rhizome node prepared in the step a by water, and then soaking and cleaning the reed rhizome node in flowing water for 60 minutes;
c. preparing rooting powder solution: weighing 100mg of ABT 1 rooting powder agent, adding the rooting powder agent into a beaker, adding 50mL of 95% ethanol for dissolution, and adding 950mL of water after dissolution to prepare 100mg/L of ABT 1 rooting powder solution;
d. rooting powder solution treatment: bundling 10 rapier reed knots treated in the step b into a bundle, and placing the bundle into a culture basin filled with 100mg/L of ABT 1 rooting powder solution prepared in the step c; then placing the culture basin in an illumination incubator for culture under the following conditions: the temperature is 25 ℃, the illumination time is 16h, the illumination intensity is 20000lx, the humidity is 60%, rooting powder solution is replaced once every 3 days, the rooting powder solution is cultivated for 6 days, the solution is kept to submerge axillary bud nodes of the reed rhizome node in the whole process (3 repeats are arranged in the test, 20 reed rhizome nodes are arranged in 2 bundles of each repeat, in this step, clear water treatment is used as a control group, 3 repeats are also arranged in the control group, and 20 reed rhizome nodes are arranged in 2 bundles of each repeat);
e. preparing a compound fertilizer solution: 1000mL of water is measured, 0.25g of a macroelement water-soluble fertilizer of kefeng is added, and the mixture is stirred until the mixture is completely dissolved, so as to obtain a compound fertilizer solution with the concentration of 0.25 g/L;
f. and (3) solution culture of compound fertilizer: transferring the reed rhizome node obtained after the treatment in the step d into the compound fertilizer solution prepared in the step e for culture, wherein the culture conditions are as follows: the temperature is 25 ℃, the illumination time is 16 hours, the illumination intensity is 20000lx, the humidity is 60%, the compound fertilizer solution is replaced every 3 days, the total culture is carried out for 14 days, and the solution is kept to submerge the axillary bud node of the reed rhizome node for 1-2 cm in the whole process.
The specific operation of the embodiment is as follows: the axillary buds on the reed rhizome nodes germinate into buds, then new roots grow at the base parts of the buds, the new roots can be observed to grow at the earliest 10 th day, the number of the reed rhizome nodes rooted for 1 time is counted every 2 days, and the rooting rate, the root number and the root length of each reed rhizome node are counted at the 20 th day. In general, each reed rhizome node has only one bud, and very individual reed rhizome nodes have two or more buds, in which case, the total number of all the roots on the reed rhizome node is taken as the root number of the reed rhizome node, the root length average of new roots growing on each bud of the reed rhizome node is taken as the root length of the reed rhizome node, and the root rate, root number and root length of each repetition are calculated by Microsoft Excel according to the following public expressions (1) - (3).
Rooting rate = number of rooted reed rhizome nodes/total reed rhizome node number x 100% (1)
Root number = total number of roots/number of rooted reed rhizome knots (2)
Root length = root length sum of each reed rhizome/number of rooted reed rhizome (3)
The results show that in the embodiment of the invention, the rooting rate of the reed rhizome nodes reaches 89.79% after 16 days of culture, and the rooting rate of the control group is 76.22%. At day 20, rooting rate was as high as 96.75% in the examples of the present invention, and 89.60% in the control group. Furthermore, it was found by statistical analysis of the root number and root length that there was no significant difference in root length of each reed rhizome node of 6.04cm and 6.02cm between the treatment group and the control group in the example of the present invention on day 20. However, there was a very significant difference in the number of roots per reed rhizome node between the treated group, which was as high as 9.15 pieces, and the control group, which was 3.59 pieces. From the results, the water culture method of the reed rhizome, disclosed by the embodiment of the invention, has the advantages of high rooting speed, high rooting rate and more roots.
Claims (2)
1. The water planting method for rapidly rooting the reed rhizome is characterized by comprising the following steps of:
a. preparing rooting powder solution: weighing 90-110 mg of rooting powder medicament, adding the rooting powder medicament into a beaker, adding 50mL of 95% ethanol for dissolution, and adding 950mL of water after dissolution to prepare 90-110 mg/L of ABT rooting powder solution;
b. rooting powder solution treatment: c, putting the reed rhizome node into the rooting powder solution prepared in the step A for treatment, wherein the rooting powder solution is 1-2 cm higher than the axillary bud node of the reed rhizome node, and in the treatment process, the temperature is controlled to be 25+/-2 ℃, the illumination time is 16-17 h, the illumination intensity is 19000-20000 lx, the treatment time is 6 days, and the rooting powder solution is replaced once every 3 days;
the main stems of the reed rhizome are culture knots, each reed rhizome is provided with an axillary bud, the length of the knots is 2-4 cm, and the length of the knots is 5-7 cm;
c. preparing a compound fertilizer solution: 1000mL of water is measured, 0.2-0.3 g of compound fertilizer is added, and the mixture is stirred until the mixture is completely dissolved, so as to obtain compound fertilizer solution with the concentration of 0.2-0.3 g/L;
the compound fertilizer is a macroelement water-soluble fertilizer for applying kefeng;
d. and (3) solution culture of compound fertilizer: transferring the reed rhizome node treated in the step b into a prepared compound fertilizer solution for culture, wherein the compound fertilizer solution is 1-2 cm higher than the axillary bud node of the reed rhizome node until rooting;
the conditions during the culture are as follows: the temperature is 25+/-2 ℃, the illumination time is 16-17 h, the illumination intensity is 19000-20000 lx, and the compound fertilizer solution is replaced every 3 days.
2. The rapid rooting water planting method for reed canary grass according to claim 1, wherein: and a rooting powder in the step a is rooting powder No. 1.
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| CN202211059752.6A CN115500252B (en) | 2022-08-31 | 2022-08-31 | Hydroponic method for rapid rooting of reed leaves and reeds |
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| CN115956501A (en) * | 2023-01-10 | 2023-04-14 | 郑州大学 | Rapid breeding method of asploid sword-like reed based on water culture |
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| GB201210374D0 (en) * | 2012-06-12 | 2012-07-25 | New Energy Farms | Plant propagation |
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| CN102625653A (en) * | 2009-08-13 | 2012-08-01 | 特里弗里生物量解决方案股份有限公司 | Methods for vegetative propagation of grass plants |
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