CN1471812A - Method for growing small shaddock - Google Patents
Method for growing small shaddock Download PDFInfo
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- CN1471812A CN1471812A CNA031493777A CN03149377A CN1471812A CN 1471812 A CN1471812 A CN 1471812A CN A031493777 A CNA031493777 A CN A031493777A CN 03149377 A CN03149377 A CN 03149377A CN 1471812 A CN1471812 A CN 1471812A
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Abstract
A technology for high-speed reproduciton of high-quality teak in batches includes such steps as choosing the donor of explant, collecting explants, sterilizing, initial generation culturing, secondary culturing, making seedlings strong, promoting root growth by use of efficient root promoter, test-tube plantlet transplanting, and transplanting seedlings in field. Its advantages are high reproduction rate increased from 2-3 times to 4 times, high surviral rate (98% or more), and low cost.
Description
Technical field the invention belongs to method for plant tissue culture, particularly a kind ofly reaches shaddock by biotechnology
The teak micro-propagation method of quick breeding of wood breeding and large-scale production.
Background technology teak (Tectona grandis L.) belongs to the Verenaceae Tectona, for the torrid zone tall and big
Broad-leaved half deciduous tree.The teak material is tough and tensile, compact structure, beautiful texture, corrosion resistant anti-
Worm, processing characteristics are good, are widely used in to build naval vessel, harbour, bridge, compartment, room
Room, senior floor, furniture and edge pasting board and thick china etc. are world-renowned commodity
Material is described as one of important tropical hard wood commerical tree species in the world.Teak is different flower
The pollination seeds.
Because the characteristic of teak has abundant heredity along with the difference of geographical provenance alters a great deal
Diversity, though very favourable alternative is provided, for fine-variety breeding provides hereditary basis,
Yet what China widely planted so far almost is the seedling of being bred by seed entirely.This class
Sexual progeny, its characteristic is separated serious, and forest form is irregular, and available log output is not high.
Seriously restricting the development of China's teak industry.Though introduced a fine variety over one hundred year, and do not form industrialization eventually
Promote.Main in the world teak is planted state the improved variety that realizes teak is all attached great importance to,
Make great efforts to seek the breeding cloning process, along with the development of biotechnology subject, twentieth century eight
The ten's were carried out the Study on tissue culture of teak at home and abroad in succession, and had obtained very
Gratifying progress, but domesticly do not form large-scale production, abroad also report is seldom failed
Satisfy the teak requirement of large-scale production.
Summary of the invention the present invention is intended to use biotechnology, fast a large amount of reproduction improved variety kinds in than short-term
Seedling, set up defect individual from the root clone, form complete teak breeding micro-propagation method.
The purpose of invention realizes by following scheme:
Grow the selected of body donor outward and---grow the body collection outward---growing the body sterilization outward---first generation
---successive transfer culture (enrichment culture)---strong sprout and hardening---is urged root in (startup) cultivation
Handle---the test-tube plantlet transplanting (bag seedling or dish seedling---the bag seedling)---for land for growing field crops production field planting.Below further auspiciously state the present invention:
1, selectedly outer grows body donor plant, choose and meet production requirement breeding individual plant, gather the usefulness that twig does cultivation.
2, grow outward and take back group training factory after the body collection, clean with clear water earlier, sterilized 15～20 minutes with 0.15～0.25% mercuric chloride in the gnotobasis, totally standby with sterile water wash again.
3, initial culture (startup lure bud cultivate): that will sterilize and clean up grows body (terminal bud or have the stem section of axillalry bud) to be inoculated in modified MS medium is minimal medium outward, additional basic element of cell division 6-BA (cultivates on 0.5～2mg/l) the medium, culturing room's temperature is 25～28 ℃, natural scattering light added the fluorescent lamp supplementary illumination 12～16 hours, luminous intensity 2000 ± 100Lux.
4, shoot proliferation is cultivated: when the foster aseptic bud that obtains of being commissioned to train is originally grown 6～7cm, can enter shoot proliferation, with its segmentation cutting, change over to piecemeal in the fresh proliferated culture medium and cultivate, left and right sides subculture was transferred once in per 30 days, and subculture medium is that (0.1～2mg/l)+KT (0～1mg/l) for MS+6-BA.
5, strong sprout and hardening: when test-tube plantlet up to 1.5～5cm, move to seeding room from culturing room, carry out light temperature hardening, control intensity of illumination at 5000～15000Lux.Make the test-tube plantlet stalwartness, height of seedling reaches 8cm left and right sides Shi Ke for transplanting.
6, urge root to handle: hardening is about 20 days, chooses and urges root to handle before transplanting strong sprout, and the aqua or the pulvis of urging the root agent to adopt being made up of growth hormone such as IBA, NAA, IAA are urged the root processing, and concentration respectively is 100～150ppm.Aqua need soak afterwards earlier to be transplanted, and pulvis is with gluing with kind.
7, test-tube seedling transplanting: the teak unrooted test-tube seedling transplanting of urging root to handle is cultivated to the mixed-matrix of yellow soil and sand etc., but direct transplanting becomes bagged seedlings in the plastic sack that matrix is housed; Also can transplant with vinyl disc dress matrix earlier, wait to coil to move into plastic sack again after seedling rooting grows tall, strengthen management after moving, land for growing field crops production field planting can be provided when treating height of seedling 15～40cm.Advantage of the present invention:
1, a month rate of increase is brought up to 4 times by 2～3 times, has strengthened the propagation dynamics, reaches fast numerous requirement, realizes the target of large-scale promotion plantation.
2, the unrooted test-tube plantlet is efficiently being urged under the root powder effect, direct transplanting, and saving of work and time, the survival rate of transplanting seedlings on a large scale reaches more than 98%.
Technology of the present invention reaches the purpose of low cost, low energy consumption, high efficiency, large-scale production, has practical value and promotional value, and wide application prospect is arranged.
Embodiment 1, the healthy list that selected speed is living, disease-resistant from teak forest, anti-adversity ability does very well
Strain is as gathering the explant maternal plant.
2, gather the branch that sleeping bud is arranged, as explant.
3, clean explant with clear water, on superclean bench, the mercuric chloride with 0.2%
Solution disinfection (immersion) 10-20 minute is wanted sufficiently sterilised.Use sterile water wash.
4, branch (terminal bud or branch) the segmentation access that disinfects is lured the bud medium
(MS+6BA2ppm), in 26 ± 2 ℃ culturing room, illumination 2000Lux
About.
5, cultivate about 10 days, the eruption sprouting, 20-30 days, during the long 6-7cm of twig,
Enter successive transfer culture.
6, with above-mentioned twig segment, every section band 1-2 inserts subculture medium to bud
(MS+6BA0.8ppm) about one month, continue switching again, until
Enough amounts enter strong sprout, the hardening stage.
7, choosing moves to seeding room at nature up to the test-tube plantlet of 1.5-5cm from culturing room
(5000-15000Lux) hardening makes the test-tube plantlet stalwartness under the light, leaf look viridescent,
Stem is tall and straight.
8, hardening is about 20 days, with concentration respectively be 100ppm IBA, NAA,
IAA urges the root powder to handle, and, plants mixing of yellow soil and sand etc. is being housed with kind with sticking
Close in the Seedling bag of matrix and grow seedlings, can provide the land for growing field crops to produce during height of seedling 15-40cm
Field planting.
Claims (1)
1, a kind of teak micro-propagation method is characterized in that:
1) selectedly outer grow body donor plant, choose and meet production requirement breeding individual plant, gather the usefulness that twig does cultivation,
2) take back group training factory after growing the body collection outside, clean with clear water earlier, sterilized 15～20 minutes with 0.15～0.25% mercuric chloride in the gnotobasis, totally standby with sterile water wash again,
3) initial culture: i.e. startup lures bud to cultivate, with the outer body of growing of sterilizing and cleaning up, be that to be inoculated in modified MS medium be minimal medium for terminal bud or the stem section that has axillalry bud, additional basic element of cell division 6-BA (cultivates on 0.5～2mg/l) the medium, culturing room's temperature is 25～28 ℃, natural scattering light added the fluorescent lamp supplementary illumination 12～16 hours, luminous intensity 2000 ± 100Lux
4) shoot proliferation is cultivated: when the foster aseptic bud that obtains of being commissioned to train is originally grown 6～7cm, can enter shoot proliferation, with its segmentation cutting, change over to piecemeal in the fresh proliferated culture medium and cultivate, left and right sides subculture switching in per 30 days once, subculture medium is that (0.1～2mg/l)+KT (0～1mg/l) for MS+6-BA
5) strong sprout and hardening: when test-tube plantlet up to 1.5～5cm, move to seeding room from culturing room, carry out light temperature hardening, the control intensity of illumination makes the test-tube plantlet stalwartness at 5000～15000Lux, height of seedling reaches 8cm left and right sides Shi Ke for transplanting,
6) urge root to handle: hardening is about 20 days, chooses and urges root to handle before transplanting strong sprout, and the aqua or the pulvis of urging the root agent to adopt being made up of growth hormone such as IBA, NAA, IAA are urged the root processing, concentration respectively is 100～150ppm, aqua need soak afterwards earlier to be transplanted, and pulvis is with gluing with kind
7) test-tube seedling transplanting: the teak unrooted test-tube seedling transplanting of urging root to handle is cultivated to the mixed-matrix of yellow soil and sand etc., but direct transplanting becomes bagged seedlings in the plastic sack that matrix is housed; Also can transplant with vinyl disc dress matrix earlier, wait to coil to move into plastic sack again after seedling rooting grows tall, strengthen management after moving, land for growing field crops production field planting can be provided when treating height of seedling 15～40cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031493777A CN1471812A (en) | 2003-06-25 | 2003-06-25 | Method for growing small shaddock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031493777A CN1471812A (en) | 2003-06-25 | 2003-06-25 | Method for growing small shaddock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1471812A true CN1471812A (en) | 2004-02-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031493777A Pending CN1471812A (en) | 2003-06-25 | 2003-06-25 | Method for growing small shaddock |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415890C (en) * | 2005-11-28 | 2008-09-03 | 邓子牛 | Method for regeration of citrus internode stem and genetic transformation |
| CN101250549B (en) * | 2008-04-01 | 2011-08-10 | 湖南农业大学 | Citrus mature internode stems genetic transformation method |
| CN105104054A (en) * | 2015-08-08 | 2015-12-02 | 龚卫新 | Method for cultivating teak seedling |
| CN106472215A (en) * | 2016-09-30 | 2017-03-08 | 贵州山至金生态农业有限公司 | Peach tree nursery cuttage breeding method |
| CN109006471A (en) * | 2018-06-08 | 2018-12-18 | 中国林业科学研究院热带林业研究所 | A kind of rapid propagation method of teak cotyledon adventitious bud inducing |
-
2003
- 2003-06-25 CN CNA031493777A patent/CN1471812A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415890C (en) * | 2005-11-28 | 2008-09-03 | 邓子牛 | Method for regeration of citrus internode stem and genetic transformation |
| CN101250549B (en) * | 2008-04-01 | 2011-08-10 | 湖南农业大学 | Citrus mature internode stems genetic transformation method |
| CN105104054A (en) * | 2015-08-08 | 2015-12-02 | 龚卫新 | Method for cultivating teak seedling |
| CN106472215A (en) * | 2016-09-30 | 2017-03-08 | 贵州山至金生态农业有限公司 | Peach tree nursery cuttage breeding method |
| CN109006471A (en) * | 2018-06-08 | 2018-12-18 | 中国林业科学研究院热带林业研究所 | A kind of rapid propagation method of teak cotyledon adventitious bud inducing |
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