CN111374033A - Water planting cutting seedling method for giant reed - Google Patents
Water planting cutting seedling method for giant reed Download PDFInfo
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- CN111374033A CN111374033A CN202010320314.5A CN202010320314A CN111374033A CN 111374033 A CN111374033 A CN 111374033A CN 202010320314 A CN202010320314 A CN 202010320314A CN 111374033 A CN111374033 A CN 111374033A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a method for water culture cutting seedling raising of giant reed, which specifically comprises the following steps: placing the cutting into an aqueous culture solution to be cultured until the cutting is rooted to obtain the cutting after the cutting is rooted; transplanting the rooted cutting shoots, and performing conventional management to obtain bamboo reed seedlings; wherein the formula of the aqueous culture solution is as follows: 1/5 Hoagland nutrient solution + NAA or IBA 0.14-0.16 mg/L. Compared with the conventional Arundo donax cuttage, the method has the characteristics of short seedling culture period and high rooting rate (the rooting rate reaches 90% counted from the beginning to the 28 th day of water culture); compared with the tissue culture propagation mode in the prior art, the method has the advantages of basically no investment of production equipment, low technical requirement and relatively fewer processes, and can still obtain higher rooting rate in a shorter period under the condition.
Description
Technical Field
The invention relates to a seedling raising method of plants, in particular to a water culture cutting seedling raising method of arundo donax linn.
Background
Arundo donax (Arundo donax L.) is a perennial rhizomatic plant. Has the characteristics of strong reproductive capacity, rapid growth, large biomass and the like. The giant reed has wide economic application, is not only excellent pasture and building material, but also can be used as raw material for paper-making pulping, and the pulp yield is higher than that of common wood, reed and wheat straw; as a new biomass energy plant, the biomass energy plant has low pollution emission in the combustion process, is quite environment-friendly, and can also be used for preparing ethanol, generating methane and the like. Along with the deep understanding of the giant reed by people, the application of the giant reed is more diversified, and the demand is increased day by day, so that how to improve the biomass yield of the giant reed and expand the seedling source becomes more important.
As the giant and microspores formed by meiosis of the giant reed germ cells cannot normally develop, although seeds are formed, the seeds are sterile and can only be subjected to asexual propagation, and the common propagation modes comprise tissue culture (tissue culture), tillering and cuttage. The tissue culture technology can obtain a large amount of seedlings, but has the limitations of high requirements on production equipment and technology, high cost and the like. The tillering propagation coefficient is low, and the plants are different in size and growth, so that the requirement on seedlings in production cannot be met. The cutting propagation is mainly carried out in spring or autumn by taking main stems as cutting slips, and the representative methods mainly comprise the following two methods:
(1) and (3) carrying out cuttage after the cutting slips are subjected to auxin soaking treatment:
for example, the invention patent with publication number CN106069015A discloses a cutting method of floral leaf arundo donax linn, which comprises the following steps: the pruned tender branches are used as cutting slips, are soaked in IBA (indolebutyric acid) 300-500mg/L for 5-10 seconds and then are cut in a matrix, and are maintained to root. According to the invention, the rooting rate of the cutting floral leaf giant reed is greatly improved by designing a detailed cutting treatment method and combining with the soaking treatment by using a growth regulator, and the statistical data of three months shows that the rooting rate reaches more than 89%. The invention also carries out comparative experiments on the rooting influence of various growth hormones on the arundo donax, and the results show that the significance of the influence of different concentrations of various rooting agents on the average rooting rate of the cutting shoot is IBA, ABI, GGR, NAA and IAA in sequence, wherein IBA500mg/L is the most prominent, and the rooting rate reaches 100% after the cutting shoot is threshed. However, the rooting time is too long although the rooting rate of the method is high.
Also, for example, the influence of the growth regulator on the summer cuttage survival rate of the lateral branches of the arundo donax (Rotori et al, J. biol., 03 2018, p23-26) discusses the influence of different types and concentrations of the plant growth regulator and the rooting powder (agent) on the summer cuttage survival rate of the lateral branches of the arundo donax linn. The literature indicates that the current-year lateral branches of arundo donax linn with axillary buds are taken as cutting slips, the cutting slips are soaked for 2 hours by using plant growth regulators of indolebutyric acid (IBA), naphthylacetic acid (NAA), indoleacetic acid (IAA) and rooting powder (agent) solutions with different concentrations, then cutting is carried out, the cutting slips are soaked in clear water as a control, and the survival rate of the lateral branch cutting is counted after 15 days. Results show that compared with a control, the plant growth regulators with different types and different concentrations have different degrees of promotion effects on the survival rate of the bamboo reed lateral branch cuttage, particularly the promotion effect of 0.8mg/L NAA is remarkable, and the survival rate reaches 50.00%. The rooting powder (agent) can also improve the survival rate of the cutting slips, the survival rate of 0.625g/L rooting powder treatment is 40.00 percent, the effect of 1.25g/L strong rooting agent is the best, and the survival rate is 43.33 percent. After the preferable growth regulator and the rooting powder (agent) are combined and matched, the survival rate of the cutting slips is generally reduced, and the combination with the highest survival rate (0.05mg/L of IAA and 5.0g/L of rooting agent) is only 25.00 percent. Therefore, when the giant reed is cut on the lateral branches in summer, 0.8mg/L NAA or 1.25g/L strong rooting agent is preferably selected as the soaking solution for pretreatment, so that the survival rate of cutting is effectively improved. Since the cuttings are directly cut into the matrix after being subjected to the plant growth regulator in the literature and can survive only after rooting, the survival rate counted in the literature can be regarded as the rooting rate, and thus the rooting rate of the method in the literature is less than 50%.
(2) The cutting slips are directly cut without auxin soaking treatment:
for example, the invention patent with publication number CN109006228A discloses a sugar-free rapid propagation method of arundo donax linn, which comprises the following steps: selecting lignified arundo donax stems, and cutting off the stems by taking a joint containing one joint as a unit to form arundo donax linn nodes; cutting the bamboo reed joints in a matrix, and placing the bamboo reed joints in an environment of 15-30 ℃ for moisture preservation and culture until the bamboo reed joints root; transplanting the bamboo joints, and carrying out moisturizing and shading treatment; and performing conventional seedbed management on the slub of the reed rhizome to form seedlings. Although the method has high propagation coefficient, simple process and low cost, the white root hair can be germinated in about 30 days under general conditions, and the root hair also needs time to fully develop to reach a certain root length, so that the method has longer time from cuttage to rooting and has long rooting period.
As can be seen, the existing cutting propagation mode has the defects of low rooting rate or long rooting time.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for water culture cutting seedling raising of arundo donax with high rooting rate, which can start rooting in a short time, aiming at the defects in the prior art.
In order to solve the technical problems, the water culture cutting seedling method for the arundo donax linn provided by the invention comprises the following steps: placing the cutting into an aqueous culture solution to be cultured until the cutting is rooted to obtain the cutting after the cutting is rooted; transplanting the rooted cutting shoots, and performing conventional management to obtain bamboo reed seedlings; wherein the formula of the aqueous culture solution is as follows: 1/5 Hoagland nutrient solution + NAA or IBA 0.14-0.16 mg/L.
Further, the formula of the aqueous culture solution is preferably: 1/5 Hoagland nutrient solution + NAA or IBA0.15mg/L.
In the technical scheme of the invention, the culture conditions of the cutting in the aqueous culture solution are as follows: the temperature is 25 +/-5 ℃, the illumination time is 15-18h/d, the illumination intensity is 5000-. Preferably, the illumination intensity is 5500-. When the cutting is cultured in the aqueous culture solution, the aqueous culture solution submerges the first section of the cutting from the bottom end to the top end.
In the technical scheme of the invention, the selection of the cutting is the same as that of the prior art, and specifically, the main branch, the lateral branch or the twig of the giant reed with the axillary bud can be used as the cutting, wherein the lateral branch or the twig is preferably the current-year lateral branch or the twig, and the main branch can be the current-year or biennial main branch. The length of the main branch, side branch or twig used as cutting is preferably 25-35cm, with 3-5 stem nodes.
In the technical scheme of the invention, the cutting is preferably subjected to conventional disinfection treatment before being placed in an aqueous culture solution for culture. In the application, the cutting slips are placed in 1 wt% potassium permanganate solution to be soaked for 5-10min, taken out, soaked in water for 20-30min and then placed in an aqueous culture solution to be cultured.
According to the technical scheme, the cutting slips are usually transplanted when the cutting slips are cultured in an aqueous culture solution until new buds grow to 4-6cm and new roots grow. And the transplanting is to cut the rooted cutting slips into seedling beds or seedling bags, and then manage the cuttings according to the conventional method to obtain the arundo donax seedlings. Before transplanting, the rooted cuttings are preferably subjected to conventional disinfection treatment. In the application, the rooted cutting is soaked in 1 wt% potassium permanganate solution for 5-10min, taken out, soaked in water for 20-30min and then transplanted.
Compared with the prior art, the invention is characterized in that:
1. the cutting slips are directly put into water culture solution with the formula of 1/5 Hoagland nutrient solution plus NAA or IBA of 0.14-0.16mg/L for water culture until rooting, so that the defects of low rooting rate and/or long rooting period of the Arundo donax in the cutting process in the prior art are overcome, and the cutting slips have the characteristics of short seedling culture period and high rooting rate (in the preferred embodiment, the rooting rate is counted from the beginning of water culture to the 28 th day and reaches 90%).
2. Compared with the tissue culture propagation mode in the prior art, the method has the advantages of basically no investment of production equipment, low technical requirement and relatively fewer processes, and can still obtain higher rooting rate in a shorter period under the condition.
Drawings
FIG. 1 is a photograph of cuttings from day 28 of hydroponic culture in treatment 2-3 according to example 1 of the present invention;
FIG. 2 is a photograph of Arundo donax linn transplanted into a nursery bag for 1 week after rooting in 2-3 treatments in example 1 of the present invention;
FIG. 3 is a photograph of Arundo donax linn transplanted into a nursery bag for 4 weeks after rooting in the processes 2 to 3 in example 1 of the present invention;
FIG. 4 is a photograph of Arundo donax linn transplanted into a nursery bag for 8 weeks after rooting in the treatments 2 to 3 in example 1 of the present invention.
Detailed Description
The present invention will be better understood from the following detailed description of specific examples, which should not be construed as limiting the scope of the present invention.
Example 1
The experiment is carried out in ecological building glass greenhouse of Guangxi plant research institute in Guangxi Zhuang autonomous region in 11 months in 2019.
1) Preparing cutting slips: shearing strong and healthy, disease and insect pest-free and relatively consistent growing side branches of the donax reeds with buds, removing young and tender tips and leaves on the branches, and keeping 1cm of the base parts of the leaves; cutting the lateral branches, wherein the cut at the bottom end is required to be beveled, the cut at the top end is smooth, the length of the cut lateral branches is controlled to be 25-30cm (with 3-5 stem nodes), and the lateral branches treated in the way are used as cutting slips.
2) And (3) disinfection of cutting slips: and (3) soaking the cutting in 1 wt% potassium permanganate solution for 10min (the solution does not cover the cut at the bottom end of the cutting in the soaking process), taking out, soaking in clear water for 30min, and taking out for later use.
3) Preparing a water culture nutrient solution: the water culture nutrient solution uses 1/5 Hoagland nutrient solution as a base, and growth regulators with different concentrations are added according to the table 1 for standby.
TABLE 1 Dendrocalamus latiflorus water culture nutrient solution growth regulator concentration gradient
4) Water culture: placing the disinfected cutting slips into different water culture nutrient solutions prepared in the step 3) for culturing until the cutting slips root; the culture conditions were: the temperature is 22 ℃, the illumination time is 16h/d, the illumination intensity is 5765Lux, the growth and rooting conditions of the cutting are observed and recorded every day, and the water culture solution is replaced every 7 days; during the whole culture process, the water culture solution passes through the first section of the cutting from the bottom end to the top end. The above treatments with different compositions and different concentrations were set up for 3 replicates, each treating 30 cuttings.
Observing and finding that the cutting shoots begin to bud from the 7 th day (the bud grows at the stem node, and 2-3 groups of the cutting shoots begin to bud at the earliest time), the lower parts of the cutting shoots begin to grow new roots (white root hairs, and 2-3 groups of the cutting shoots begin to grow new roots at the earliest time), the rooting rate, the number of roots on each cutting shoot and the root length are counted at the 28 th day (normally, only one bud grows on each cutting shoot, and two or more buds grow on one cutting shoot in the rare case; when two or more buds grow on one cutting shoot, the total number of roots on the lower parts of all the buds on the cutting shoot is divided by the total number of the buds to serve as the number of roots of the cutting shoot, and the average number of the root lengths of the new roots on each bud on the cutting shoot serves as the root length of the cutting shoot), the rooting rate, the number of roots and the root length of each treatment are calculated by statistical excel, The number of roots and the root length were calculated according to the following formulas (1) to (3), respectively, and the results are shown in Table 2.
The rooting rate is × 100% (1) of the number of cuttings to be rooted/total number of cuttings
Root system number (total number of roots/number of cuttings to root) (2)
Root length-total root length/total root (3)
TABLE 2 influence of growth regulators on the Water planting rooting Rate of the lateral branches of Arundo donax
5) Transplanting: and when the cutting slips are cultured in an aqueous culture solution until new buds grow to 4-6cm and new roots grow, taking out the cutting slips after rooting, soaking in 1 wt% potassium permanganate solution for 10min, then soaking in water for 30min, then transplanting into a seedling raising bag, and then managing according to a conventional method to obtain the bamboo reed seedlings.
In this example, the cutting on the 28 th day of hydroponics in the treatment 2-3 was as shown in fig. 1, the arundo donax was transplanted (the cutting was cultured in the aqueous culture solution until new shoots grew to 4-6cm and new roots grew and the cutting was transplanted) to the seedling-raising bag for 1 week (7 days) as shown in fig. 2, the arundo donax transplanted to the seedling-raising bag for 4 weeks (28 days) as shown in fig. 3, and the arundo donax transplanted to the seedling-raising bag for 8 weeks (56 days) as shown in fig. 4.
The survival rate of the seedlings transplanted into the seedling raising bags in each treatment was counted for 4 weeks, as shown in the following table 3.
TABLE 3 survival rate after 4 weeks of different treatments of transplantation
Comparative example
The experiment is carried out in ecological building glass greenhouse of Guangxi plant research institute in Guangxi Zhuang autonomous region in 11 months in 2019.
Steps 1) and 2) are the same as in example 1.
3) Preparing a water culture nutrient solution: the water culture nutrient solution formula of each treatment is prepared according to the following table 4 for standby.
TABLE 4 Dendrocalamus latiflorus aqueous culture Medium growth regulator concentration gradient
The MS culture medium in the table is a basic MS culture medium well known in the art.
4) Water culture: placing the disinfected cutting slips into different water culture solutions prepared in the step 3) for culturing until the cutting slips root; the culture conditions were the same as in example 1. The above different treatment settings were repeated 3 times, each treating 30 cuttings.
It was observed that the cuttings began to bud at the earliest day 9 (bud growth was at the stem node, and the hoagland nutrient solution treatment group began to bud at the earliest day), new roots began to grow at the earliest day 13 (white root hair, and new roots began to grow at the earliest day in the hoagland nutrient solution treatment group), and the rooting rate, the number of roots per cutting and the length of roots were counted at day 28 (the counting method was the same as that of example 1), which is specifically shown in table 4.
Example 2
The experiment is carried out in ecological building glass greenhouse of Guangxi plant research institute in Guangxi Zhuang autonomous region in 11 months in 2019.
Steps 1) and 2) are the same as in example 1.
3) Preparing a water culture nutrient solution: the formula of the water culture nutrient solution is 1/5 Hoagland nutrient solution and NAA0.16mg/L.
4) Water culture: placing the disinfected cutting slips into different water culture solutions prepared in the step 3) for culturing until the cutting slips root; the culture conditions are as follows: the temperature is 25 ℃, the illumination time is 15h/d, the illumination intensity is 5500Lux, the growth and rooting conditions of the cutting are observed and recorded every day, and the water culture solution is replaced every 7 days; during the whole culture process, the water culture solution passes through the first section of the cutting from the bottom end to the top end. 3 replicates were set up, each treating 30 cuttings.
It was observed that the cutting shoots bud from day 7 (shoot length at stem node), new roots grow from the lower part of the shoot on day 11, and the rooting rate, the number of roots per cutting shoot and the root length were counted on day 28 (the statistical method is the same as example 1), and the results are: the rooting rate is 87%, the number of roots is 2.27, and the root length is 5.53 cm.
Example 3
The experiment is carried out in ecological building glass greenhouse of Guangxi plant research institute in Guangxi Zhuang autonomous region in 11 months in 2019.
1) Preparing cutting slips: pruning strong and healthy biennial giant reed main branches with bud points without diseases and insect pests and consistent growth vigor, removing lateral branch tips and leaves on the branches, and keeping 1cm of leaf base; cutting off the main branches, wherein the cut at the bottom end is required to be beveled, the cut at the top end is smooth, the length of the cut main branches is controlled to be 30-35cm (with 3-5 stem nodes), and the main branches processed in the way are used as cutting slips.
2) And (3) disinfection of cutting slips: the same as in example 1.
3) Preparing a water culture nutrient solution: the formula of the water culture nutrient solution is 1/5 Hoagland nutrient solution and IBA0.14mg/L.
4) Water culture: placing the disinfected cutting slips into different water culture solutions prepared in the step 3) for culturing until the cutting slips root; the culture conditions are as follows: the temperature is 20 ℃, the illumination time is 18h/d, the illumination intensity is 6000Lux, the growth and rooting conditions of the cutting are observed and recorded every day, and the water culture solution is replaced every 7 days; during the whole culture process, the water culture solution passes through the first section of the cutting from the bottom end to the top end. 3 replicates were set up, each treating 30 cuttings.
It was observed that the cutting shoots bud from day 7 (shoot length at stem node), new roots grow from the lower part of the shoot on day 10, and the rooting rate, the number of roots per cutting shoot and the root length were counted on day 28 (the statistical method is the same as example 1), and the results are: the rooting rate is 85%, the number of roots is 2.17, and the root length is 5.54 cm.
Claims (5)
1. The method for culturing the seedlings of the arundo donax by water culture cutting is characterized in that cutting slips are placed in a water culture solution to be cultured until the cutting slips take roots, and the cutting slips after the rooting are obtained; transplanting the rooted cutting shoots, and performing conventional management to obtain bamboo reed seedlings; wherein the formula of the aqueous culture solution is as follows: 1/5 Hoagland nutrient solution + NAA or IBA 0.14-0.16 mg/L.
2. The method of claim 1, wherein the aqueous broth formulation is: 1/5 Hoagland nutrient solution + NAA or IBA0.15 mg/L.
3. The method according to claim 1 or 2, wherein the cultivation conditions of the cutting in the aqueous culture solution are: the temperature is 25 +/-5 ℃, the illumination time is 15-18h/d, the illumination intensity is 5000-.
4. The method according to claim 1 or 2, wherein the cutting is cultured in an aqueous culture solution, and the aqueous culture solution is submerged in the first node of the cutting starting from the bottom end toward the top end.
5. The method according to claim 1 or 2, wherein the main branch, side branch or twig of Arundo donax with axillary buds is used as the cutting.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
| CN115500252A (en) * | 2022-08-31 | 2022-12-23 | 郑州大学 | Water culture method for rapid rooting of arundo donax linn |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946620A (en) * | 2010-08-27 | 2011-01-19 | 南京中科水治理工程有限公司 | Method for propagating potamogeton malaianus |
| CN102450150A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Propagation technology for potamogeton pectinatus L |
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN103875469A (en) * | 2012-12-20 | 2014-06-25 | 盐城工学院 | Method for quickly evaluating salt tolerance of grapes |
| CN106386445A (en) * | 2016-12-06 | 2017-02-15 | 中国科学院地理科学与资源研究所 | Rooting method of Hylotelephium erythrostictum by using water culture and cutting |
| CN107223425A (en) * | 2017-06-05 | 2017-10-03 | 山东农业大学 | A kind of method for promoting fruit vegetables cuttage seeding adventitious root to generate |
-
2020
- 2020-04-22 CN CN202010320314.5A patent/CN111374033A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946620A (en) * | 2010-08-27 | 2011-01-19 | 南京中科水治理工程有限公司 | Method for propagating potamogeton malaianus |
| CN102450150A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Propagation technology for potamogeton pectinatus L |
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN103875469A (en) * | 2012-12-20 | 2014-06-25 | 盐城工学院 | Method for quickly evaluating salt tolerance of grapes |
| CN106386445A (en) * | 2016-12-06 | 2017-02-15 | 中国科学院地理科学与资源研究所 | Rooting method of Hylotelephium erythrostictum by using water culture and cutting |
| CN107223425A (en) * | 2017-06-05 | 2017-10-03 | 山东农业大学 | A kind of method for promoting fruit vegetables cuttage seeding adventitious root to generate |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
| CN112753393B (en) * | 2020-12-31 | 2022-04-22 | 华南农业大学 | Broussonetia papyrifera root propagation method |
| CN115500252A (en) * | 2022-08-31 | 2022-12-23 | 郑州大学 | Water culture method for rapid rooting of arundo donax linn |
| CN115500252B (en) * | 2022-08-31 | 2024-02-27 | 郑州大学 | Hydroponic method for rapid rooting of reed leaves and reeds |
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