CN102450150A

CN102450150A - Propagation technology for potamogeton pectinatus L

Info

Publication number: CN102450150A
Application number: CN2010105254542A
Authority: CN
Inventors: 柴夏; 刘平平; 史加达; 呙明杰; 刘从玉; 万陆军; 陈小峰; 谢超
Original assignee: NANJING ZHONGKE WATER ENVIRONMENT ENGINEERING Co Ltd
Current assignee: NANJING ZHONGKE WATER ENVIRONMENT ENGINEERING Co Ltd
Priority date: 2010-11-01
Filing date: 2010-11-01
Publication date: 2012-05-16
Anticipated expiration: 2030-11-01
Also published as: CN102450150B

Abstract

The invention discloses a propagation method for submerged plant potamogeton pectinatus L, comprising the following steps: culturing the potamogeton pectinatus L in the Hoagland culture solution; inducing roots to grow when adding 0.5-2mg/L of indolebutyric acid; and inducing lateral buds to grow when adding 0.5-2mg/L of 6-benzylaminopurine. After being cultured for 15-20 days indoors, the potamogeton pectinatus L is transplanted to a seedling field to be cultured for 30-35 days; after the plant grows to 20-30cm, the potamogeton pectinatus L is transplanted to the target water. With simple operation and high survival rate, the propagation technology for potamogeton pectinatus L solves the problem that the explants of the potamogeton pectinatus L cannot root when being propagated.

Description

Comb tooth pondweed propagation technique

Technical field

The invention belongs to biological technical field, be specifically related to the propagation method of a kind of submerged plant comb tooth pondweed.

Background technology

Comb tooth pondweed (Potamogeton pectinatus L) is a kind of perennial herb submerged plant, in China each province distribution is arranged all.Have another name called: pondgrass, fennelleaf pondweed herb.Comb tooth pondweed Potamogeton is in Potamogetonaceae (Potamogetonaceae), Potamogeton (Potamogeton).Along with the aggravation of lake eutrophication, submerged plant was fewer and feweri in the last few years.For the effectively deterioration of containment water quality, people have taked a series of engineering measure, and planting planting submerged plant recovery aquatic vegetation is the domestic commonly used and apparent in view engineering measure of effect.Comb tooth pondweed is to recover one of aquatic vegetation kind commonly used.

The propagation method of breeding comb tooth pondweed is mainly the hibernaculum breeding at present.Hibernaculum breeding operation is comparatively simple, but the hibernaculum in the natural lake is fewer and feweri, can't satisfy wilderness demand at all.Comb tooth pondweed utilization cottage method is bred has certain difficulty, and the direct cuttage of explant can't grow root system for a long time, can't become alive.Therefore want to make the cuttage explant to become to live, the root system that its growth is made new advances is very crucial.

Summary of the invention

The purpose of invention: present technique has been to provide the propagation method of a kind of submerged plant comb tooth pondweed; Comb tooth pondweed is cultivated in Huo Gelan (Hoagland) culture fluid; Under the situation of adding indolebutyric acid 0.5～2mg/L, induce the generation of root system, induce the growth of lateral bud under the situation of interpolation 6-benzyl aminoadenine 0.5～2mg/L.Indoor cultivation is transplanted to the field of growing seedlings and was cultivated 30～35 days after 15～20 days, both can be transplanted to the target water body when treating plant strain growth to 20～30 centimetre, and transplanting survival rate is more than 90%.

The invention meaning: comb tooth pondweed adaptability is strong, and it is wide to distribute, and is the plant variety that aquatic vegetation recovers and water pollution control is commonly used.Comb tooth pondweed well developed root system, plant is longer, can effectively suppress the release of endogenous pollution, improves water quality.

Technical scheme of the present invention:

1, the collection growth conditions is good, the comb tooth pondweed plant of no damage by disease and insect.

2, gather the back and clean 2～3 times, rinse out the bright surface of plant attachment with clear water.

3, explant is cut into 10～15 centimeter length.

4, the preparation of Huo Gelan (Hoagland) culture fluid

Huo Gelan (Hoagland) basic element prescription:

Four water-calcium nitrate 945mg/L

Potassium nitrate 506mg/L

Ammonium nitrate 80mg/L

Potassium dihydrogen phosphate 136mg/L

Magnesium sulfate 493mg/L

Ferrous sulfate heptahydrate 13.9mg/L

Disodium ethylene diamine tetraacetate 18.65mg/L

KI 0.00415mg/L

Boric acid 0.031mg/L

Manganese sulphate 0.1115mg/L

Zinc sulphate 0.043mg/L

Sodium molybdate 0.00125mg/L

Copper sulphate 0.000125mg/L

Cobalt chloride 0.000125mg/L

5, in Huo Gelan (Hoagland) culture fluid, add indolebutyric acid 0.5～2mg/L, 6-benzyl aminoadenine 0.5～2mg/L.

6, will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates.In room temperature is 25 ℃, than being 1: 1, cultivates 10～15 days round the clock.

7, treat that newborn root system grows to 1～2 centimetre, lateral budding grows to 3～5 centimetres, it is taken out from culture fluid, with flushing with clean water 1 time.Planting the field of growing seedlings cultivates.

8, the water in field that keeps growing seedlings is dark more than 30 centimetres, and 15 ℃～30 ℃ of water temperatures were cultivated 20～25 days, and its plant grows to 20～30 centimetres promptly can be as kind of a transplantation of seedlings.

Know-why: in Huo Gelan (Hoagland) nutrient solution,, breed seedling through cuttage then through the growth of hormone induction promotion root and bud.Gibberellin is the one type of plant hormone that extensively exists, and can stimulate the growth of leaf and bud; 6-benzyl aminoadenine also is a kind of plant hormone, can promote cell differentiation and division; Help the differentiation of new root generation and fibrovascular system, promote the formation of cutting adventive root, be mainly used in rooting of cuttings; Can induce the formation of root substance, be widely used in the cuttage root-taking of trees, flowers.

Beneficial effect: present technique has effectively solved comb tooth pondweed breeding problem, and the advantage of present technique is simple to operate, can breed a large amount of comb tooth pondweed seedlings in a short time; Can effectively solve the phenomenon that cuttage seeding is not taken root, improve the survival rate of cuttage seeding, and can breed a large amount of seedlings in a short time, satisfy the needs of engineering a large amount of seedlings.

Four, embodiment:

Through concrete embodiment the present invention is further specified below; Should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the principle of the invention; Can also make some modification and improvement, these also should be regarded as belonging to protection scope of the present invention.

Case study on implementation: the propagation method of a kind of tooth pondweed of combing of the present invention comprises the steps:

2, gather the back and clean 3 times, rinse out the bright surface of plant attachment with clear water.

3, comb tooth pondweed plant is cut into 10～15 centimeter length explants.

4, the preparation of Huo Gelan (Hoagland) culture fluid.Huo Gelan (Hoagland) culture fluid comprises: Huo Gelan (Hoagland) basic element, indolebutyric acid 1mg/L, 6-benzyl aminoadenine 1mg/L.

5, will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates.In room temperature is 25 ℃, than being 1: 1, cultivates 15 days round the clock.

6, long 2 centimetres of newborn root system after 15 days, 5 centimetres of lateral bud growths are taken out it from culture fluid, with flushing with clean water 1 time.Planting the field of growing seedlings cultivates.

7, keep 40 centimetres of the depth of waters, 15～30 ℃ of water temperatures were cultivated 22 days, and its plant grows to 30 centimetres and transplants.

Cultivate 10000 strains of comb tooth pondweed through said method, using artificial lake water is given birth to vegetation recovery engineering, survival rate 92%.

Claims

1. the propagation method of tooth pondweed of combing is characterized in that, comprises the steps:

(1), to gather growth conditions good, the comb tooth pondweed plant of no damage by disease and insect;

(2), gather the back and clean 3～5 times with clear water, rinse out the bright surface of plant attachment;

(3), each explant of explant that comb tooth pondweed plant is cut into 10～15 centimeter length comprises 2～3 internodes;

(4), the preparation of Huo Gelan (Hoagland) culture fluid.Huo Gelan (Hoagland) culture fluid comprises: Huo Gelan (Hoagland) basic element, plant hormone;

(5), will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates.In room temperature is 25 ℃, than being 1: 1, cultivates 10～15 days round the clock;

(6), treat that newborn root system grows to 1～3 centimetre, lateral budding grows to 5 centimetres, it is taken out from culture fluid, with flushing with clean water 1 time.Planting the field of growing seedlings cultivates;

(7), keep 30 centimetres of the depth of waters, 15 ℃～30 ℃ of water temperatures were cultivated 20～25 days, its plant grows to 20～30 centimetres promptly can be as kind of a transplantation of seedlings.

2. the propagation method of comb tooth pondweed according to claim 1 is characterized in that step (4) Huo Gelan (Hoagland) culture fluid contains indolebutyric acid 0.5～2mg/L, 6-benzyl aminoadenine 0.5～2mg/L.