CN102450150B - Propagation technology for potamogeton pectinatus L - Google Patents
Propagation technology for potamogeton pectinatus L Download PDFInfo
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- CN102450150B CN102450150B CN 201010525454 CN201010525454A CN102450150B CN 102450150 B CN102450150 B CN 102450150B CN 201010525454 CN201010525454 CN 201010525454 CN 201010525454 A CN201010525454 A CN 201010525454A CN 102450150 B CN102450150 B CN 102450150B
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Abstract
The invention discloses a propagation method for submerged plant potamogeton pectinatus L, comprising the following steps: culturing the potamogeton pectinatus L in the Hoagland culture solution; inducing roots to grow when adding 0.5-2mg/L of indolebutyric acid; and inducing lateral buds to grow when adding 0.5-2mg/L of 6-benzylaminopurine. After being cultured for 15-20 days indoors, the potamogeton pectinatus L is transplanted to a seedling field to be cultured for 30-35 days; after the plant grows to 20-30cm, the potamogeton pectinatus L is transplanted to the target water. With simple operation and high survival rate, the propagation technology for potamogeton pectinatus L solves the problem that the explants of the potamogeton pectinatus L cannot root when being propagated.
Description
Technical field
The invention belongs to biological technical field, be specifically related to the propagation method of a kind of submerged plant comb tooth pondweed.
Background technology
Comb tooth pondweed (Potamogeton pectinatus L) is a kind of perennial herb submerged plant, in China each province, distribution is arranged all.Have another name called: pondgrass, fennelleaf pondweed herb.Comb tooth pondweed Potamogeton is in Potamogetonaceae (Potamogetonaceae), Potamogeton (Potamogeton).Along with the aggravation of lake eutrophication, submerged plant was fewer and feweri in the last few years.For the effectively deterioration of containment water quality, people have taked a series of engineering measure, and planting planting submerged plant recovery aquatic vegetation is the domestic commonly used and apparent in view engineering measure of effect.Comb tooth pondweed is to recover one of aquatic vegetation kind commonly used.
The propagation method of breeding comb tooth pondweed is mainly the hibernaculum breeding at present.Hibernaculum breeding operation is comparatively simple, but the hibernaculum in natural lake is fewer and feweri, can't satisfy wilderness demand at all.Comb tooth pondweed utilization cottage method is bred has certain difficulty, and the explant cuttage can't grow root system for a long time, can't survive.Therefore want to make the cuttage explant to survive, the root system that its growth is made new advances is very crucial.
Summary of the invention
The purpose of invention: present technique has been to provide the propagation method of a kind of submerged plant comb tooth pondweed, comb tooth pondweed is cultivated in Huo Gelan (Hoagland) culture fluid, in the situation that add the generation that indolebutyric acid 0.5～2mg/L induces root system, induce the growth of lateral bud in the situation of interpolation 6-benzyl aminoadenine 0.5～2mg/L.Indoor cultivation is transplanted to the field of growing seedlings and cultivated 30～35 days after 15～20 days, both can be transplanted to the target water body when plant to be planted grows to 20～30 centimetres, and transplanting survival rate is more than 90%.
The invention meaning: comb tooth pondweed strong adaptability, distribute wide, be the plant variety that aquatic vegetation recovers and water pollution control is commonly used.Comb tooth pondweed well developed root system, plant is longer, can effectively suppress the release of endogenous pollution, improves water quality.
Technical solution of the present invention:
1, the collection growth conditions is good, without the comb tooth pondweed plant of damage by disease and insect.
2, clean 2～3 times with clear water after gathering, rinse out plant bright surface attachment.
3, explant is cut into 10～15 centimeter length.
4, the preparation of Huo Gelan (Hoagland) culture fluid
Huo Gelan (Hoagland) basic element formula:
Four water-calcium nitrate 945mg/L
Potassium nitrate 506mg/L
Ammonium nitrate 80mg/L
Potassium dihydrogen phosphate 136mg/L
Magnesium sulfate 493mg/L
Ferrous sulfate heptahydrate 13.9mg/L
Disodium ethylene diamine tetraacetate 18.65mg/L
Potassium iodide 0.00415mg/L
Boric acid 0.031mg/L
Manganese sulphate 0.1115mg/L
Zinc sulphate 0.043mg/L
Sodium molybdate 0.00125mg/L
Copper sulphate 0.000125mg/L
Cobalt chloride 0.000125mg/L
5, add indolebutyric acid 0.5～2mg/L, 6-benzyl aminoadenine 0.5～2mg/L in Huo Gelan (Hoagland) culture fluid.
6, will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates.Be 25 ℃ in room temperature, than being 1: 1, cultivated 10～15 days round the clock.
7, treat that newborn root system grows to 1～2 centimetre, lateral budding grows to 3～5 centimetres, and it is taken out from culture fluid, rinses 1 time with clear water.Plantation is cultivated to the field of growing seedlings.
8, the water in field that keeps growing seedlings is dark more than 30 centimetres, and 15 ℃～30 ℃ of water temperatures were cultivated 20～25 days, and its plant grows to 20～30 centimetres namely can be as kind of a transplantation of seedlings.
Know-why: promote the growth of root and bud by hormone induction in Huo Gelan (Hoagland) nutrient solution, then breed seedling by cuttage.Gibberellin is a class plant hormone that extensively exists, and can stimulate the growth of leaf and bud; 6-benzyl aminoadenine is also a Plant Hormone, can promote Cell Differentiation and division, be conducive to the differentiation of new root generation and fibrovascular system, promote the formation of cutting adventive root, be mainly used in rooting of cuttings, can induce the formation of root substance, be widely used in the cuttage root-taking of trees, flowers.
Beneficial effect: present technique has effectively solved comb tooth pondweed breeding problem, and the advantage of present technique is simple to operate, can breed in a short time a large amount of comb tooth pondweed seedlings; Can effectively solve the phenomenon that cuttage seeding is not taken root, improve the survival rate of cuttage seeding, and can breed a large amount of seedlings in a short time, satisfy engineering to the needs of a large amount of seedlings.
Four, embodiment:
The present invention is further described below by specific embodiment; should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the principle of the invention; can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Case study on implementation: the propagation method of a kind of tooth pondweed of combing of the present invention comprises the steps:
1, the collection growth conditions is good, without the comb tooth pondweed plant of damage by disease and insect.
2, clean 3 times with clear water after gathering, rinse out plant bright surface attachment.
3, comb tooth pondweed plant is cut into 10～15 centimeter length explants.
4, the preparation of Huo Gelan (Hoagland) culture fluid.Huo Gelan (Hoagland) culture fluid comprises: Huo Gelan (Hoagland) basic element, indolebutyric acid 1mg/L, 6-benzyl aminoadenine 1mg/L.
5, will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates.Be 25 ℃ in room temperature, than being 1: 1, cultivated 15 days round the clock.
6, after 15 days, newborn root system is grown 2 centimetres, and 5 centimetres of lateral bud growths are taken out it from culture fluid, rinse 1 time with clear water.Plantation is cultivated to the field of growing seedlings.
7, keep 40 centimetres of the depth of waters, 15～30 ℃ of water temperatures were cultivated 22 days, and its plant grows to 30 centimetres and transplants.
Cultivate 10000 strains of comb tooth pondweed by said method, using artificial lake water is given birth to vegetation recovery engineering, survival rate 92%.
Claims (1)
1. the propagation method of tooth pondweed of combing, is characterized in that, comprises the steps:
(1), to gather growth conditions good, without the comb tooth pondweed plant of damage by disease and insect;
(2), clean 3～5 times with clear water after gathering, rinse out plant bright surface attachment;
(3), comb tooth pondweed plant is cut into the explant of 10～15 centimetres, each explant comprises 2～3 internodes;
(4), the preparation of Huo Gelan (Hoagland) culture fluid: Huo Gelan (Hoagland) culture fluid comprises: Huo Gelan (Hoagland) basic element, plant hormone; Described plant hormone is indolebutyric acid 0.5～2mg/L, 6-benzyl aminoadenine 0.5～2.0mg/L;
(5), will shear good explant puts into Huo Gelan (Hoagland) culture fluid and cultivates; Be 25 ℃ in room temperature, than being 1: 1, cultivated 10～15 days round the clock;
(6), treat that newborn root system grows to 1～3 centimetre, lateral budding grows to 5 centimetres, and it is taken out from culture fluid, rinses 1 time with clear water, plantation is to the field cultivation of growing seedlings;
(7), keep 30 centimetres of the depth of waters, 15 ℃～30 ℃ of water temperatures were cultivated 20～25 days, its plant grows to 20～30 centimetres namely as the kind transplantation of seedlings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010525454 CN102450150B (en) | 2010-11-01 | 2010-11-01 | Propagation technology for potamogeton pectinatus L |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010525454 CN102450150B (en) | 2010-11-01 | 2010-11-01 | Propagation technology for potamogeton pectinatus L |
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| CN102450150A CN102450150A (en) | 2012-05-16 |
| CN102450150B true CN102450150B (en) | 2013-05-22 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053422B (en) * | 2013-01-09 | 2014-03-26 | 成都华都农业发展有限责任公司 | Method for reproducing potamogeton perfoliatus |
| CN104094843B (en) * | 2014-05-16 | 2016-08-17 | 南京中科水治理股份有限公司 | The propagation method of hornwort and application thereof |
| CN104705194A (en) * | 2015-04-03 | 2015-06-17 | 南京大学 | Hormone-induced rapid propagation method of potamogeton pectinatus engineering seedlings |
| CN105493871A (en) * | 2016-01-06 | 2016-04-20 | 纪效峰 | Cultivation method of camphor leaf grass |
| CN106954549B (en) * | 2017-02-27 | 2019-05-28 | 水生藻安生物科技（武汉）有限公司 | A kind of comb tooth bog pondweed tissue culture and rapid proliferation method |
| CN107371680A (en) * | 2017-06-30 | 2017-11-24 | 南京中科水治理股份有限公司 | A kind of breeding method for strengthening the domestication of Potamogeton pectinatus L salt resistance ability |
| CN109601156A (en) * | 2019-01-16 | 2019-04-12 | 中国水产科学研究院珠江水产研究所 | A kind of comb tooth bog pondweed cuttage propagation method |
| CN110122304A (en) * | 2019-06-14 | 2019-08-16 | 福建农林大学 | A kind of method of banana water planting hardening |
| CN111374033A (en) * | 2020-04-22 | 2020-07-07 | 广西壮族自治区中国科学院广西植物研究所 | Water planting cutting seedling method for giant reed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1561687A (en) * | 2004-03-20 | 2005-01-12 | 中国科学院水生生物研究所 | Method for aseptic cultivating submerged plant |
| CN1596601A (en) * | 2004-09-15 | 2005-03-23 | 中国科学院南京地理与湖泊研究所 | Method of planting |
| CN1930953A (en) * | 2006-10-13 | 2007-03-21 | 中国科学院武汉植物园 | Fast propagation process of potarnogeton lucens |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1561687A (en) * | 2004-03-20 | 2005-01-12 | 中国科学院水生生物研究所 | Method for aseptic cultivating submerged plant |
| CN1596601A (en) * | 2004-09-15 | 2005-03-23 | 中国科学院南京地理与湖泊研究所 | Method of planting |
| CN1930953A (en) * | 2006-10-13 | 2007-03-21 | 中国科学院武汉植物园 | Fast propagation process of potarnogeton lucens |
Non-Patent Citations (4)
| Title |
|---|
| 李洪林等.沉水植物竹叶眼子菜的组织培养和快速繁殖.《植物生理学通讯》.2007,第43卷(第2期), |
| 沉水植物竹叶眼子菜的组织培养和快速繁殖;李洪林等;《植物生理学通讯》;20070430;第43卷(第2期);第329页 * |
| 蓖齿眼子菜繁殖多样性研究;陈开宁等;《植物生态学报》;20030531;第27卷(第5期);第672-676页 * |
| 陈开宁等.蓖齿眼子菜繁殖多样性研究.《植物生态学报》.2003,第27卷(第5期), |
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| CN102450150A (en) | 2012-05-16 |
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Address after: 210008 Xuanwu District, Jiangsu, Beijing East Road, No. 73, No. Applicant after: Nanjing Zhongke Water Environment Engineering Co., Ltd. Address before: 210008 Xuanwu District, Jiangsu, Beijing East Road, No. 73, No. Applicant before: Nanjing Zhongke Water Environment Engineering Co., Ltd. |
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