CN102450149A - Cutting propagation method for potamogeton crispus L - Google Patents
Cutting propagation method for potamogeton crispus L Download PDFInfo
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- CN102450149A CN102450149A CN2010105254523A CN201010525452A CN102450149A CN 102450149 A CN102450149 A CN 102450149A CN 2010105254523 A CN2010105254523 A CN 2010105254523A CN 201010525452 A CN201010525452 A CN 201010525452A CN 102450149 A CN102450149 A CN 102450149A
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- water caltrop
- caltrop
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Abstract
The invention relates to a method for inducing radical buds to propagate potamogeton crispus L by using hormones, belonging to the biotechnology field. Roots are promoted to grow by using naphthylacetic acid and buds are promoted to grow by using 6-benzylaminopurine so as to improve cutting survival rate. The operation steps are as follows: (1), collecting stem sections for propagating; (2), preparing and processing stem sections processing drug; and (3), cutting stem sections and managing seedling fields. Compared with winter bud propagation and tissue culture propagation, the method provided in the invention has the advantages of simple operation, low culture condition demands, low investment cost, short propagation period and the like; the potamogeton crispus L propagation cost is greatly reduced; and the method meets the demand that a great number of potamogeton crispus L is needed when recovering aquatic vegetation.
Description
Technical field
The invention belongs to biological technical field, be specifically related to the cottage propagation technology of a kind of submerged plant water caltrop.
Background technology
The perennial heavy pasture and water basis of water caltrop (Potamogeton crispus L): be shrimp algae, shrimp grass again.Potamogetonaceae (Potamogetonaceae), Potamogeton (Potamogeton).The water caltrop life cycle is different with most water plants, and it germinates in the fall, the Winter-Spring growth, and yield positive results 4～May, and decline is rotten gradually after June in summer, forms the squama branch simultaneously to spend summer.
Along with the pollution aggravation of water quality, seldom seen large-area water caltrop growth at natural world, the plantation water caltrop has crucial meaning in aquatic vegetation recovery and body eutrophication improvement engineering.The death that decayed in the winter time such as other submerged plant such as eel grass, black algae, p.malaianus in the winter time, the existence of water caltrop has replenished the primary productivity of aquatic ecosystem in winter, can guarantee the water stabilization in winter.Therefore plant water caltrop to winter aquatic ecosystem stable very important.
The water caltrop modes of reproduction is mainly leaned on the breeding of squama branch at present, and reproduction speed is slow, and the collection of squama branch is difficulty relatively.Therefore need a kind of can the breeding in a large number, cost is low again, propagation method simple to operate.
Summary of the invention
The purpose of invention: the invention provides the water caltrop cuttage breeding method, low and difficult the taking root with the direct cuttage survival rate of water caltrop adopts hormone induction to handle, and the water caltrop rooting rate is remained on more than 90%, and survival rate 85%, growth rate are not obviously accelerated before handling.Effectively solved the problem of the sapling multiplication of water caltrop, this method is simple to operate, and cost is low.
The meaning of invention: water caltrop to winter water ecological environment stable very big effect is arranged, so water caltrop often is used to carry out aquatic vegetation and recovers and body eutrophication improvement.
Main technical flows comprises: the collection of (), water caltrop stem section; (2), handle the preparation and the processing of medicament; (3), the cuttage of water caltrop stem section and the field management of growing seedlings.Concrete steps are following:
1, gathers water caltrop stem section, select the stem section of the vigorous no damage by disease and insect of growth, clean 2～3 times, remove surface attachments with clear water.
2, water caltrop stem section is cut into 10～15 centimeter length.
3, handle the preparation of medicament: add 1～5 milligram of methyl, 1～3 milligram of 6-benzyl aminoadenine in every liter of distilled water.
4, will shear good water caltrop stem section is immersed in explant and handled in the medicament 1 hour.
5, the water caltrop that will soak is with flushing with clean water 1 time, and to the Tanaka that grows seedlings, seeding row spacing is respectively 10 centimetres with its cuttage.
6, grow seedlings that water in field is dark to keep 0.3～0.5 meter, water temperature was cultivated 30～45 days for 15 ℃～25 ℃, treated that it grows to 30 centimetres and both can be transplanted to the target water body.Carry out above operation and both can obtain a large amount of water caltrop seed and seedlings.
Embodiment
A kind of water caltrop hormone induction cuttage breeding method, embodiment is:
1, from irrigation canal, gathers the water caltrop plant, select the plant of the vigorous no damage by disease and insect of growth,, remove its surface attachments with flushing with clean water 3 times.
2, with cleaned water caltrop with cutting into 10～15 centimeter length.
3, will shear good water caltrop stem section and be immersed in and contain 1 milligram of methyl, in the distilled water that 6-benzyl aminoadenine is 1 milligram 1 hour.
4, will soak water caltrop after 1 hour with flushing with clean water 1 time, with its cuttage to the Tanaka that grows seedlings.
5, the cuttage seeding row spacing of water caltrop is 10 centimetres.Keep 0.4 meter of the depth of water, cultivated 30 days, 20 ℃ of water temperature average out to, cultivating survival rate is 89%.
6, when the plant average length is 30 centimetres, to be transplanted in the landscape lake, the depth of water is 2 meters, well-grown, survival rate 95%.
Claims (3)
1. a water caltrop (Potamogeton crispus L) cuttage breeding method, this method may further comprise the steps:
(1), the water caltrop stem section of gathering the vigorous no damage by disease and insect of growth, clean 2～3 times with clear water and remove surface attachments.
(2), water caltrop stem section is cut into 10～15 centimeter length.
(3), will shear good water caltrop stem section is immersed in and handled in the medicament 1 hour.
(4), the water caltrop that will soak is with flushing with clean water 2～3 times, with its cuttage to the Tanaka that grows seedlings.
(5), keep 0.3 meter of the depth of water, cultivated 30 days, treat that plant grows to 30 centimeter length, both can be transplanted to the plantation water body.
2. handle the compound method of medicament in the claim 1: add 1～5 milligram of methyl, 1～3 milligram of 6-benzyl aminoadenine in every liter of distilled water.
3. the water caltrop that method according to claim 1 cultivates out in the lake, the water correction and the aquatic ecosystem reparation of river, wetland and manmade landscape, artificial landscape's water body make up application in engineering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105254523A CN102450149A (en) | 2010-11-01 | 2010-11-01 | Cutting propagation method for potamogeton crispus L |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105254523A CN102450149A (en) | 2010-11-01 | 2010-11-01 | Cutting propagation method for potamogeton crispus L |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102450149A true CN102450149A (en) | 2012-05-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010105254523A Pending CN102450149A (en) | 2010-11-01 | 2010-11-01 | Cutting propagation method for potamogeton crispus L |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941157A (en) * | 2016-06-13 | 2016-09-21 | 中国科学院亚热带农业生态研究所 | Potamogeton malainus single-stem-fragment fast propagation method |
| CN105993535A (en) * | 2016-06-13 | 2016-10-12 | 中国科学院亚热带农业生态研究所 | Rapid expanding propagation method for potamogeton crispus single-section stem fragments |
| CN108812093A (en) * | 2018-06-04 | 2018-11-16 | 华中农业大学 | A kind of field water caltrop implantation methods that rice shrimp makees altogether |
| CN109601156A (en) * | 2019-01-16 | 2019-04-12 | 中国水产科学研究院珠江水产研究所 | A kind of comb tooth bog pondweed cuttage propagation method |
| CN113812313A (en) * | 2021-09-29 | 2021-12-21 | 上海同瑞环保工程有限公司 | Method for planting part of submerged plant with growth capacity |
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| CN1742563A (en) * | 2005-10-13 | 2006-03-08 | 华中师范大学 | Method for fast breeding water caltrop seedlings |
| CN1943324A (en) * | 2005-10-13 | 2007-04-11 | 华中师范大学 | Method for fast breeding water caltrop seed and seedling |
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Patent Citations (4)
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| CN1545861A (en) * | 2003-12-08 | 2004-11-17 | �Ϻ���ͨ��ѧ | Method for cultivating curly pondweed embryo and curly pondweed karren tissue |
| CN1701661A (en) * | 2003-12-08 | 2005-11-30 | 南京大学 | Curly pondweed rigid bud tissue culturing method |
| CN1742563A (en) * | 2005-10-13 | 2006-03-08 | 华中师范大学 | Method for fast breeding water caltrop seedlings |
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| 安彦杰等: "沉水植物菹草的人工种子技术", 《水生生物学报》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941157A (en) * | 2016-06-13 | 2016-09-21 | 中国科学院亚热带农业生态研究所 | Potamogeton malainus single-stem-fragment fast propagation method |
| CN105993535A (en) * | 2016-06-13 | 2016-10-12 | 中国科学院亚热带农业生态研究所 | Rapid expanding propagation method for potamogeton crispus single-section stem fragments |
| CN105941157B (en) * | 2016-06-13 | 2018-02-02 | 中国科学院亚热带农业生态研究所 | A kind of quick propagation method of p.malaianus Single stem segment |
| CN105993535B (en) * | 2016-06-13 | 2020-05-08 | 中国科学院亚热带农业生态研究所 | Rapid propagation method for potamogeton crispus single-section stem segments |
| CN108812093A (en) * | 2018-06-04 | 2018-11-16 | 华中农业大学 | A kind of field water caltrop implantation methods that rice shrimp makees altogether |
| CN109601156A (en) * | 2019-01-16 | 2019-04-12 | 中国水产科学研究院珠江水产研究所 | A kind of comb tooth bog pondweed cuttage propagation method |
| CN113812313A (en) * | 2021-09-29 | 2021-12-21 | 上海同瑞环保工程有限公司 | Method for planting part of submerged plant with growth capacity |
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Application publication date: 20120516 |