CN105993535B - Rapid propagation method for potamogeton crispus single-section stem segments - Google Patents
Rapid propagation method for potamogeton crispus single-section stem segments Download PDFInfo
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- CN105993535B CN105993535B CN201610410374.XA CN201610410374A CN105993535B CN 105993535 B CN105993535 B CN 105993535B CN 201610410374 A CN201610410374 A CN 201610410374A CN 105993535 B CN105993535 B CN 105993535B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
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Abstract
The invention discloses a rapid propagation method of potamogeton crispus single-section stem sections, which comprises the following steps: (1) drawing materials in the field: selecting a potamogeton crispus community with better growth potential in the shallow water wetland, and collecting potamogeton crispus stems without diseases and insect pests by using a tool; (2) selecting stem sections: washing the field stem with tap water, and cutting the stem into stem segments with complete internodes with scissors; (3) soaking the rooting powder solution: putting the cut stem segments into a rooting powder solution for soaking; (4) preparing a matrix: washing fine sand with tap water for later use; (5) preparing a culture vessel: laying the culture substrate on a culture vessel; (6) cultivation: vertically inserting the selected stem segments into the matrix; (7) culturing: adding water once a day, wherein the water amount is kept to cover 2-3 cm of the top end of the stem section; (8) transplanting: taking out the plants from the matrix, and transplanting the plants into natural water. The method is easy to implement, simple and convenient to operate, short in propagation period (about 20 days), high in branching rate of over 95%, high in rooting rate of over 90% and high in transplanting survival rate of 95%.
Description
Technical Field
The invention belongs to the field of biotechnology, and particularly relates to a method for rapidly expanding and propagating potamogeton crispus stems, which is suitable for wetland restoration and the construction of artificial wetland and aquatic bases for the requirement of a large number of high-quality potamogeton crispus seedlings.
Background
Curly pondweed (Potamogeton crispus L.), alias shrimp and algae, shrimp and grass, and wheat yellow grass, etc. are distributed in most areas of China, and submerged herbaceous plants are grown for many years. It is mainly in pond, lake and stream, in still water pond or ditch, and its water body is slightly acid or neutral, and its curly pondweed stem is flat and round, and has branches. The leaves are needle-shaped, the tip is blunt and round, and the leaf edges are wavy and have saw teeth. Has a leaf support and no leaf stalk. The inflorescence is spike-shaped. Sprouting in autumn, growing in winter and spring, blooming and bearing in 4-5 months, gradually declining and decaying after 6 months in summer, and forming scale branches (winter buds) to spend uncomfortable environment. The winter bud is hard, the edge of the winter bud is provided with teeth, the shape of the winter bud is like that of a pine cone, and the winter bud starts to germinate and grow when the water temperature is proper. Leaf bar shape without handle. The flower and fruit period is 4-7 months. Can be used as green manure and can purify water.
The potamogeton crispus has a cylindrical shape, a slightly flat and multi-branched stem, the root part near the root is usually crawled on the ground, sparse or dense root hairs and leaf strips are grown at joints, and the stem is long and many joints are formed. The said potamogeton crispus has obvious effect in increasing milk secretion and milk fat rate of milk cow, egg laying rate of poultry, hatchability and hatching egg fertility rate, and can be directly used for feeding fish, it is a green feed which is fond of fish such as grass carp, megalobrama amblycephala and bushy bream, etc. and said potamogeton crispus has high heavy metal enriching capacity, and can be cultivated for about one month by using zinc-containing mixed waste water, its in vivo zinc content is greater than 8 times of original zinc content, and its arsenic-cleaning capacity is stronger, and under the condition of cultivating the arsenic acid-containing dipotassium hydrogen mixed waste water, its in vivo arsenic content can be greater than 16 times of original arsenic content. Along with the implementation of projects such as returning to lake for wetting in the middle and lower reaches of Yangtze river, wetland ecological restoration, artificial wetland, wetland park construction and the like, the demand on potamogeton crispus seedlings is more and more urgent. However, under natural conditions, curly pondweed mainly propagates through the stone buds, with very few branches arising from the stems. Ecological restoration, wetland park construction and the like are carried out by adopting a method of salvaging stone buds in lakes and ditches, which is labor-consuming and time-consuming and greatly damages the ecological system of the local wetland. Therefore, the invention is necessary to provide a large amount of high-quality potamogeton crispus seedlings by a rapid and efficient expanding propagation method.
Through the search of Chinese patent web and related thesis websites, some reports about potamogeton crispus reproduction technology are found at present, for example, in the ' potamogeton crispus cutting reproduction method ' (application publication No. CN 102450149A), potamogeton crispus ' and the like, the method of cutting is mentioned for seedling; yan 21165in "method for quick reproduction of potamogeton crispus seedling" (application publication No. CN 1742563) refers to the tissue culture and seedling culture of potamogeton crispus, etc.. However, the number of materials to be prepared is large, the steps are complicated, and the materials are difficult to understand and grasp for ordinary people.
Disclosure of Invention
The invention aims to provide a method for rapidly expanding potamogeton crispus single-section stem segments, which is easy to implement and simple and convenient to operate, and has the advantages of short expanding and propagating period (about 20 days), 90% of rooting rate, 95% of branching rate and 95% of transplanting survival rate. The method can rapidly provide a large amount of high-quality potamogeton crispus seedlings, has low cost, can obtain a large amount of high-quality potamogeton crispus seedlings in a short period, and meets the requirements of wetland ecological restoration, wetland parks, artificial wetlands, aquatic bases and the like.
In order to achieve the purpose, the invention adopts the following technical measures:
a method for rapidly expanding and propagating single-section stem segments of potamogeton crispus comprises the following steps:
(1) drawing materials in the field: selecting the potamogeton crispus colony with good growth in 80-150 cm shallow water wetland, collecting potamogeton crispus stems without diseases and insect pests, carefully placing into a bucket with 10-20cm water depth, and soaking the stems in water to keep the wet state.
(2) Selecting stem sections: washing water caltrop stem collected from wild with tap water for 2-3 times, and removing surface and attachments. The long stem is cut into stem segments with a length of 3-4cm with scissors, and each stem segment is guaranteed to have a complete internode.
(3) Soaking the rooting powder solution: putting the cut curly pondweed stem into 2g/L rooting powder solution (2 g of rooting powder is dissolved in 1L of tap water) to be soaked for 2 hours.
The rooting powder mainly comprises 90% of naphthylacetic acid raw powder, and the rest 10% of talcum powder serving as a filling agent.
(4) Preparing a culture substrate: washing fine sand with diameter of 0.5-1 mm with tap water.
The culture medium is a solid medium which replaces soil to provide mechanical support for plants, is free of nutrition, has neutral pH and has no influence on experiments.
(5) Preparing a culture vessel: placing the culture vessel in a greenhouse, spreading the culture medium on the culture vessel with a thickness of 3-4cm, and adding tap water to control the water level at 10-15 cm.
(6) Cultivation: vertically inserting the selected stem into matrix (with depth of 0.8-1.1 cm), and vertically fixing the potamogeton crispus stem on the matrix to ensure that the potamogeton crispus stem is in water.
The culture vessel is placed in a greenhouse, the room temperature is set to be 20-23 ℃, the illumination intensity is 2000-4000 lux, and the illumination time is 10-12 h/day.
(7) Culturing: the water is replenished once a day, and the water amount is kept to cover 2-3 cm of the top end of the stem segment. When water is added, the water flow should not be too large so as to avoid stem sections floating on the water surface caused by substrate washing. After 6-8 days, the segments grow roots and generate new branches, the branching rate reaches over 95 percent within 2 weeks, the rooting rate reaches over 90 percent, and 5-10 new leaves grow on each branch on average.
(8) Transplanting: after 3 weeks, 2-3 branches grow on the stem segment, 5-10 leaves of each branch, the stem node and the new branch take roots, and the plants are taken out from the matrix and transplanted into natural water bodies (lakes, ponds, ditches and the like).
Compared with the prior art, the invention establishes a technical system for rapidly expanding and propagating potamogeton crispus, and has the following advantages and effects:
(1) the potamogeton crispus is widely distributed in shallow lakes, ponds, ditches and other water areas, and has a large amount of raw materials which are easy to obtain;
(2) the rooting period of the branches is short, and only about 20 days are needed from culture to transplantation;
(3) the branch rate of the stem section is high and reaches 95 percent, and the rooting rate is also as high as 90 percent;
(4) the number of leaves on each branch is more, and the average number of leaves of each branch is up to 5-10;
(3) the transplanting survival rate is high and is 95 percent;
(4) the preparation method has the advantages of few materials to be prepared, easy market purchase, low cost and easy mastering of technology, and can be used for large-scale production of seedlings.
Detailed Description
The invention is further illustrated, but is not to be construed as being limited by the following examples
Example 1:
a method for rapidly expanding potamogeton crispus single-section stem segments comprises the following steps:
1. drawing materials in the field: selecting the potamogeton crispus colony in 80 or 100 or 130 or 150cm shallow water wetland, selecting the potamogeton crispus colony with good growth potential in potamogeton lake wetland, cutting the potamogeton crispus stem sections floating in water body with sickle, etc., selecting the stem sections without plant diseases and insect pests, putting them into prepared plastic bucket, putting them into the bucket with 10 or 14 or 17 or 20cm water depth, adding a small amount of lake water (determined according to the size of the plastic bucket), and soaking the potamogeton crispus in water to keep the moist state.
2. Selecting stem sections: washing the rhizome of Potamogeton crispus collected from wild with tap water for 2 or 3 times, and removing surface and attachments. The long stem is cut with scissors into 3 or 4cm long stem segments and each stem segment is guaranteed to have a complete internode.
3. Soaking the rooting powder solution: putting the cut curly pondweed stem into 2g/L rooting powder solution (2 g of rooting powder is dissolved in 1L of tap water) to be soaked for 110 or 115 or 120 or 125 or 130 minutes, and taking out for later use.
The rooting powder mainly comprises 90% of naphthylacetic acid raw powder, and the rest 10% of talcum powder serving as a filling agent.
4. Preparing a culture vessel: washing 0.5 or 0.8 or 1mm quartz sand with tap water as matrix, loosening and ventilating the solid matrix, fixing and rooting potamogeton crispus, and easily taking out during transplanting, spreading the washed quartz sand on a culture vessel with thickness of 3 or 4 or 5cm, and adding tap water to control water level at 10 cm. Then, the prepared curly pondweed stem is vertically and lightly inserted into the quartz sand (depth 0.8 or 1 or 1.1cm), the culture vessel is placed in a greenhouse, the room temperature is set to be 20 or 21 or 22 or 23 ℃, the illumination intensity is 2000 or 3000 or 4000lux, and the illumination time is 10 or 11 or 12 h/day.
5. Culturing: the water was replenished once a day, adding water to keep covering the top 2 or 3cm of the rhizome segment. The water flow should not be too large during watering so as to avoid stem sections floating on the water surface caused by substrate washing. Rooting and generating new branches after 6 or 7 or 8 days, wherein the branching rate reaches over 95 percent within 2 weeks, the branches are 3-8cm long, the rooting rate is over 90 percent, and the roots are 2-5cm long.
6. Transplanting: after 3 weeks 2 or 3 branches are reached in the stem, 6 or 7 or 8 leaves per branch, new branches are rooted, at which time the plants can be taken out of the matrix and transplanted into a pond with a survival rate of 95%.
Claims (1)
1. A method for rapidly expanding potamogeton crispus single-section stem segments comprises the following steps:
(1) drawing materials in the field: taking water caltrop community in 80 or 100 or 130 or 150cm shallow water wetland, selecting water caltrop community with better growth potential in the water caltrop lake wetland, cutting water caltrop section floating in water body with sickle tool, selecting the section without plant diseases and insect pests, putting into prepared plastic bucket, putting into bucket with 10 or 14 or 17 or 20cm water depth, and soaking water caltrop in water to keep wet state;
(2) selecting stem sections: washing the rhizome of Potamogeton crispus collected from wild with tap water for 2 or 3 times, and removing surface attachments; cutting the long stem into stem sections with the length of 3 or 4cm by using scissors, and ensuring that each stem section has a complete internode;
(3) soaking the rooting powder solution: putting the cut curly pondweed stem into 2g/L rooting powder solution, the rooting powder solution is prepared by dissolving 2g of rooting powder in 1L of tap water; soaking for 110 or 115 or 120 or 125 or 130 minutes, and taking out for later use;
the rooting powder comprises 90% of naphthylacetic acid raw powder, and the rest 10% of talcum powder;
(4) preparing a culture vessel: washing quartz sand fine sand of 0.5 or 0.8 or 1mm with tap water as matrix, loosening and ventilating the solid matrix, facilitating fixation and root-pricking of potamogeton crispus, and easily taking out the quartz sand fine sand during transplantation, spreading the washed quartz sand fine sand on a culture vessel with thickness of 3 or 4 or 5cm, and adding tap water to control water level at 10 cm; then, vertically and lightly inserting the prepared curly pondweed stem into the quartz sand fine sand with the depth of 0.8 or 1 or 1.1cm, placing the culture vessel in a greenhouse, setting the room temperature at 20 or 21 or 22 or 23 ℃, setting the illumination intensity at 2000 or 3000 or 4000lux, and setting the illumination time at 10 or 11 or 12 h/day;
(5) culturing: supplementing water once a day, and keeping covering the top end of the rhizome section for 2 or 3 cm; the water flow is not too large when watering, so as to avoid stem sections floating and exposed on the water surface caused by flushing the substrate; rooting and generating new branches after 6 or 7 or 8 days, wherein the branching rate reaches over 95 percent within 2 weeks, the branches are 3-8cm long, the rooting rate is over 90 percent, and the roots are 2-5cm long;
(6) transplanting: after 3 weeks 2 or 3 branches are reached on the stem, 6 or 7 or 8 leaves per branch, new branches are rooted, plants are taken out of the matrix and transplanted into a pond, and the survival rate reaches 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610410374.XA CN105993535B (en) | 2016-06-13 | 2016-06-13 | Rapid propagation method for potamogeton crispus single-section stem segments |
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| CN201610410374.XA CN105993535B (en) | 2016-06-13 | 2016-06-13 | Rapid propagation method for potamogeton crispus single-section stem segments |
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| CN105993535B true CN105993535B (en) | 2020-05-08 |
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| CN109937722B (en) * | 2019-03-12 | 2020-12-01 | 中国科学院华南植物园 | Method for rapidly propagating Chinese lizardtail ground stems |
| CN113812313A (en) * | 2021-09-29 | 2021-12-21 | 上海同瑞环保工程有限公司 | Method for planting part of submerged plant with growth capacity |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450149A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Cutting propagation method for potamogeton crispus L |
| CN102893781A (en) * | 2012-09-24 | 2013-01-30 | 林平 | Stevia rebaudiana single-joint half-stem cottage method |
| CN103918461A (en) * | 2014-04-28 | 2014-07-16 | 中国科学院亚热带农业生态研究所 | Single root stock bud fast propagation expanding method for miscanthus sacchariflorus |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450149A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Cutting propagation method for potamogeton crispus L |
| CN102893781A (en) * | 2012-09-24 | 2013-01-30 | 林平 | Stevia rebaudiana single-joint half-stem cottage method |
| CN103918461A (en) * | 2014-04-28 | 2014-07-16 | 中国科学院亚热带农业生态研究所 | Single root stock bud fast propagation expanding method for miscanthus sacchariflorus |
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