CN105941157A - Potamogeton malainus single-stem-fragment fast propagation method - Google Patents
Potamogeton malainus single-stem-fragment fast propagation method Download PDFInfo
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- CN105941157A CN105941157A CN201610410375.4A CN201610410375A CN105941157A CN 105941157 A CN105941157 A CN 105941157A CN 201610410375 A CN201610410375 A CN 201610410375A CN 105941157 A CN105941157 A CN 105941157A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a potamogeton malainus single-stem-fragment fast propagation method. The method comprises the steps of 1, outdoor material taking, wherein potamogeton malainus community in shallow wetlands is selected, and stems of potamogeton malainus without pest or disease damage are acquired; 2, stem segment picking, wherein the acquired stems are washed with tap water and cut into fragments with scissors; 3, rooting powder solution soaking, wherein the stem fragments are soaked in rooting powder solution; 4, medium preparation, wherein quartz sand is washed with tap water to be used; 5, culture dish preparation, wherein a culture medium is spread on a culture dish; 6, planting, wherein the stem fragments are vertically inserted into the medium; 7, culture, wherein water is added once every day with the top end of each stem fragment covered with water; 8, transplantation, wherein young plants are taken out of the medium and transplanted into natural water. The method is easy and convenient to operate, propagation period is short, bifurcation rate is 95% or more, rooting rate is 95% or more, and transplantation survival rate reaches 90%.
Description
Technical field
The invention belongs to biological technical field, be more particularly to the quick propagation method of the stem section of a kind of p.malaianus,
It is applicable to wetland recovery, artificial swamp and the Aquatic product construction of base demand to a large amount of high-quality p.malaianus seedlings.
Background technology
P.malaianus (Potamogeton wrightii Morong), call potamogeton malaianus, spur Herba potamogetonis distincti etc.,
Potamogetonaceae, Potamogeton.Perennial heavy water or floating leaf draft, subterraneous stem is flourishing.Leaf wire lanceolar or oblong, 8～
12cm length, 2.5～3cm width, vein 2, hence it is evident that, yellow, there are long handle, stipule film quality.Only have submerged leaf, the anxious point of tip, leaf margin
Wavy, there is irregular sawtooth, petiole length 2～5cm.Raw or the false axil in spike top is raw；4 pieces of stamen, carpel 3～4 pieces, brown
Color.Really Caput Anas domestica shape, 2.4mm, glossy, ridge 3, isabelline.It is born in the harder lake of geology, pond and river course, can make
Green manure and feedstuff.
P.malaianus be for many years capture leaf or heavy pasture and water this.Rhizome is flourishing, white, has fibrous root at joint.Stem is cylindrical,
Diameter about 2 millimeters, not branch or tool.There are some researches show, p.malaianus can replace Herba potamogetonis distincti's hyoscine, cures mainly acute conjunctiva
Inflammation, jaundice, edema, leucorrhea, have effect of heat-clearing and toxic substances removing, diuresis, removing food stagnancy.P.malaianus nutritive value is higher, by fresh weight
Meter, containing crude protein 13.6%, crude fat 1.6%, crude fibre 16.0%, nitrogen-free extract 43.4%, be herbivorous fishes bait and
Pig, the good feedstuff of duck.In submerged plant, Ctenopharyngodon idellus is maximum to the selection coefficient of p.malaianus.The underground of p.malaianus
Root stock is the object that the winter birds such as crane class, swan like most pick food.The absorbability of water plant heavy metal is: submerged plant
> floating leaf, fluitante > emergent aquactic plant.The copper content of p.malaianus is 1.67 times of Herba Caricis Phacotae, and zinc content is Herba Caricis Phacotae
2.72 again.In Poyang Lake, p.malaianus, copper, Zn content are up to 0.64～23.9mg/kg and 9.45～125.00 respectively
Mg/kg, is 35～3990 times and 21～446 times of content in lake water.P.malaianus is pollution sensibility plant, to various sewage
There is higher detergent power.Along with restore farmland to lakes the wettest, wetland eco-reconstruction, artificial swamp, wetland park of the middle and lower reach of Yangtze River is built
If it is etc. the enforcement of engineering, more and more urgent to the demand of p.malaianus seedling.But, under field conditions (factors), p.malaianus
Relying primarily on nourishes and generates realizes renewal and the extension of population, sometimes nourishes and generates and is difficult to survival, and requires higher skill
Art.Therefore, inventing a kind of can expanding propagation method quick, the efficient a large amount of high-quality p.malaianus seedlings of offer be necessary.
Through Chinese patent net and correlative theses retrieved web, find seldom to be related to p.malaianus Fast-propagation skill at present
The report of art, only sees the patent (application publication number about " propagation method of a kind of p.malaianus " in bavin summer
CN101946620A)；The technology such as the cuttage mentioning the tissue culture about p.malaianus, outer implant.But step is the most numerous
Trivial, and need certain technical operation, indigestion and grasp for common people.
Summary of the invention
It is an object of the invention to there are provided the quick propagation method of a kind of p.malaianus list stem segment, method is easy
OK, easy and simple to handle, in the inventive method expanding propagation cycle short (about 20 days), rooting rate reaches 95%, and branch rate reaches 95%, transplanting survival rate
Reach 90%.Can quickly provide a large amount of high-quality p.malaianus seedling, and low cost by the method, can obtain big in a short time
Amount high-quality p.malaianus seedling, meets the needs of the construction such as wetland ecological restoration, wetland park, artificial swamp, Aquatic product base.
In order to realize above-mentioned purpose, the present invention uses techniques below measure:
Single stem segment quick propagation method of a kind of p.malaianus, the steps include:
(1) draw materials in field: chooses growing way preferable p.malaianus group in 1m-3m shallow water wetland, gathers without pest and disease damage Malaysia
The stem of Herba potamogetonis distincti, is carefully placed in the bucket filling the 30-40cm depth of water, makes stem be soaked in water and keeps moisture state.
(2) select stem section: the stem tap water of the p.malaianus adopted in field rinses 2-3 time, remove dead leaf, clearly
Except surface earth and attachment.With shears, long stem is cut into the stem section of a length of 3-5cm, and ensures each stem Duan Youyi
Individual complete internode.
(3) rooting powder solution is soaked: the rooting powder solution that the p.malaianus stem section sheared is placed in 1 g/L is (raw by 1g
Root powder is dissolved in 1L tap water) in soak 110-130 minute.
Described root-inducing powder main component is the former powder of naphthalene acetic acid of 90%, and residue 10% is Pulvis Talci, does filler and uses.
(4) culture matrix is prepared: by the quartz sand of diameter 0.5-1mm tap water wash clean, standby.
It is the solid dielectric that plant provides that machinery is supported that above-mentioned culture matrix refers to for soil, without nutrition, PH in
Property, on experiment without impact.
(5) culture vessel prepares: culture vessel is placed in greenhouse, is laid in culture vessel by culture matrix, thickness
For 3-4cm, it is subsequently adding tap water controlling water level at 10-15cm.
(6) cultivation: the stem section chosen is inserted perpendicularly into substrate (degree of depth 0.8-1.1 cm), makes p.malaianus stem section hang down
Directly it is fixed in substrate, it is ensured that p.malaianus stem is all in water.
Culture vessel being placed in greenhouse, room temperature is set to 20-23 DEG C, and intensity of illumination is 2000-4000lux, illumination
Time is 12-14h/ days.
(7) cultivating: supplement a water yield every day, the water yield is to keep covering stem section top 2-3cm.When adding water, current are unsuitable
Excessive, in order to avoid washing away substrate to cause that stem section is floating exposes to the water surface.Divide in taking root in 7-9 days deutomerite and producing new branch, 3 weeks
Branch rate reaches more than 95%, and rooting rate reaches more than 95%, and average each branch grows 3-5 sheet young leaves.
(8) transplant: grow on 1-2 branch, each branch 3-5 sheet leaf, stipes and new branch raw after 3 weeks in stem section
Root, root length 8-13cm, now, plant is taken out from substrate, transplant in natural water (lake, pond, irrigation canals and ditches etc.), move
Plant survival rate 90%.
The present invention establishes the technical system of a fast-propagation p.malaianus, and the present invention compared with prior art, has
Have the advantage that and effect:
(1) p.malaianus is distributed widely in the waters such as shallow lake, pond, irrigation canals and ditches, and raw material is many and easily obtains；
(2) branch, cycle of taking root are short, only need about 20 days from cultivating to transplant；
(3) stem section branch rate is high, reaches 95%, and rooting rate is up to 95% too；
(4) branch blade number is more, and average each branch number of blade is up to 3-5 sheet；
(3) transplanting survival rate is high, is 90%；
(4) material that need to prepare is few and market is readily available, low cost, and technology is easily grasped, and can be used for large-scale production seedling.
Detailed description of the invention
Further illustrate the present invention below in conjunction with embodiment rather than limit the present invention
Embodiment 1:
The quick propagation method of a kind of p.malaianus list stem segment, the steps include:
1. draw materials in field: taking the p.malaianus group in 1m or 2m or 3m shallow water wetland, in choosing conversation in Dongting Lake area, growing way is relatively
Good p.malaianus group, extracts the p.malaianus stem section floated in water body, selects wherein with the instrument such as reaping hook, shears
Without the stem section of pest and disease damage, put in off-the-shelf Plastic Drum, bucket fills 30 or 34 or 37 or 40cm lake water (according to Plastic Drum
Size depending on), make p.malaianus be soaked in water holding moisture state.
2. select stem section: the p.malaianus rhizome tap water adopted in field rinses 2 or 3 times, remove surface earth
And attachment, extract dead leaf.With shears, long stem is cut into the stem section of a length of 3 or 4cm, and ensures each stem Duan Youyi
Complete internode.
3. soak rooting powder solution: the rooting powder solution that the p.malaianus stem section sheared is placed in 1 g/L is (raw by 1g
Root powder is dissolved in 1L tap water) in soak 110 or 115 or 120 or 125 or 130 minutes, take out standby.
Described root-inducing powder main component is the former powder of naphthalene acetic acid of 90%, and residue 10% is Pulvis Talci, does filler and uses.
4. culture vessel prepares: the tap water wash clean of the quartz sand by 0.5 or 0.8 or 1mm uses as substrate, this
Planting solid matrix loose ventilative, beneficially p.malaianus is fixing takes root, and easily takes out, by washed quartz when transplanting
Sanding flat and be laid in culture vessel, thickness is 3 or 4 or 5cm, adds tap water controlling water level at 10cm.Then by ready horse
Carry out Herba potamogetonis distincti's stem section and insert (degree of depth 0.8 or 1 or 1.1 cm) in quartz sand the most gently, culture vessel is placed in greenhouse, room
Temperature is set to 20 or 21 or 22 or 23 DEG C, and intensity of illumination is 2000 or 3000 or 4000lux, light application time be 12 or 13 or
14h/ days.
5. cultivating: supplement a water yield every day, amount of water is to keep covering rhizome section top 2 or 3cm.Current when watering
Unsuitable excessive, in order to avoid washing away substrate to cause that stem section is floating exposes to the water surface.Take root in 7 or 8 or 9 days deutomerite and produce new branch, 3
In week, branch rate reaches more than 95%, long branch 3-6cm, rooting rate more than 95%, root length 8-13cm.
6. transplant: reach 1 or 2 branch, each branch 3 or 4 or 5 leaves after 3 weeks on stem, new branch is taken root, now
Plant can be taken out from substrate, transplant in pond, survival rate 90%.
Claims (1)
1. a single stem segment quick propagation method for p.malaianus, the steps include:
(1) draw materials in field: chooses the p.malaianus group in 1m-3m wetland, gathers the stem without pest and disease damage p.malaianus,
Put in the bucket filling the 30-40cm depth of water, make stem be soaked in water holding moisture state；
(2) select stem section: the stem tap water of the p.malaianus adopted in field rinses 2-3 time, remove dead leaf, remove table
Face earth and attachment, be cut into the stem section of a length of 3-5cm with shears by long stem, each stem Duan Youyi complete joint
Between；
(3) rooting powder solution is soaked: p.malaianus stem section is placed in the rooting powder solution of 1 g/L immersion 110-130 and divides
Clock；
Described root-inducing powder composition is the former powder of naphthalene acetic acid of 90%, and residue 10% is Pulvis Talci；
(4) culture matrix is prepared: by the quartz sand of diameter 0.5-1mm tap water wash clean, standby；
Above-mentioned culture matrix refers to the solid dielectric providing machinery to support for plant for soil, and without nutrition, PH is neutrality；
(5) culture vessel prepares: culture vessel being placed in greenhouse, be laid in culture vessel by culture matrix, thickness is 3-
4cm, is subsequently adding tap water controlling water level at 10-15cm；
(6) cultivation: stem section is inserted perpendicularly into substrate, degree of depth 0.8-1.1 cm, makes p.malaianus stem section be perpendicularly fixed at substrate
On, p.malaianus stem is all in water；
Culture vessel being placed in greenhouse, room temperature is set to 20-23 DEG C, and intensity of illumination is 2000-4000lux, light application time
For 12-14h/ days；
(7) cultivating: supplement a water yield every day, the water yield covers stem section top 2-3cm, 7-9 days deutomerite is taken root and produces new
Branch；
(8) transplant: grow in stem section after 3 weeks and take root on 1-2 branch, each branch 3-5 sheet leaf, stipes and new branch, root
Long 8-13cm, takes out plant from substrate, transplants in natural water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410375.4A CN105941157B (en) | 2016-06-13 | 2016-06-13 | A kind of quick propagation method of p.malaianus Single stem segment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410375.4A CN105941157B (en) | 2016-06-13 | 2016-06-13 | A kind of quick propagation method of p.malaianus Single stem segment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105941157A true CN105941157A (en) | 2016-09-21 |
| CN105941157B CN105941157B (en) | 2018-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610410375.4A Expired - Fee Related CN105941157B (en) | 2016-06-13 | 2016-06-13 | A kind of quick propagation method of p.malaianus Single stem segment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954549A (en) * | 2017-02-27 | 2017-07-18 | 水生藻安生物科技（武汉）有限公司 | A kind of comb tooth bog pondweed tissue culture and rapid proliferation method |
| CN109984040A (en) * | 2019-04-15 | 2019-07-09 | 北京林业大学 | A kind of the stem section quick propagation method and equipment of hornwort |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946620A (en) * | 2010-08-27 | 2011-01-19 | 南京中科水治理工程有限公司 | Method for propagating potamogeton malaianus |
| CN102450149A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Cutting propagation method for potamogeton crispus L |
-
2016
- 2016-06-13 CN CN201610410375.4A patent/CN105941157B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946620A (en) * | 2010-08-27 | 2011-01-19 | 南京中科水治理工程有限公司 | Method for propagating potamogeton malaianus |
| CN102450149A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Cutting propagation method for potamogeton crispus L |
Non-Patent Citations (1)
| Title |
|---|
| 周厚高: "《园林苗圃学》", 31 August 2014, 中国农业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954549A (en) * | 2017-02-27 | 2017-07-18 | 水生藻安生物科技（武汉）有限公司 | A kind of comb tooth bog pondweed tissue culture and rapid proliferation method |
| CN106954549B (en) * | 2017-02-27 | 2019-05-28 | 水生藻安生物科技（武汉）有限公司 | A kind of comb tooth bog pondweed tissue culture and rapid proliferation method |
| CN109984040A (en) * | 2019-04-15 | 2019-07-09 | 北京林业大学 | A kind of the stem section quick propagation method and equipment of hornwort |
| CN109984040B (en) * | 2019-04-15 | 2020-12-18 | 北京林业大学 | Rapid stem propagation method for Goldfish algae |
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| Publication number | Publication date |
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| CN105941157B (en) | 2018-02-02 |
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Granted publication date: 20180202 |