CN101946620A - Method for propagating potamogeton malaianus - Google Patents
Method for propagating potamogeton malaianus Download PDFInfo
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- CN101946620A CN101946620A CN 201010265492 CN201010265492A CN101946620A CN 101946620 A CN101946620 A CN 101946620A CN 201010265492 CN201010265492 CN 201010265492 CN 201010265492 A CN201010265492 A CN 201010265492A CN 101946620 A CN101946620 A CN 101946620A
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- malaianus
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- 240000004590 Potamogeton malaianus Species 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000001902 propagating effect Effects 0.000 title abstract 2
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 10
- 239000003448 gibberellin Substances 0.000 claims abstract description 10
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 238000011010 flushing procedure Methods 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 9
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 239000003375 plant hormone Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 239000008239 natural water Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000000644 propagated effect Effects 0.000 abstract 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 16
- 238000010899 nucleation Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000035584 blastogenesis Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012851 eutrophication Methods 0.000 description 3
- 150000002505 iron Chemical class 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- LBLSRDDHGILUJH-UHFFFAOYSA-N 4-(1h-indol-2-yl)butanoic acid Chemical class C1=CC=C2NC(CCCC(=O)O)=CC2=C1 LBLSRDDHGILUJH-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000722195 Potamogeton Species 0.000 description 2
- 241000756999 Potamogetonaceae Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for propagating potamogeton malaianus. The method comprises the following steps of: selecting and sterilizing an explant; preparing culture solution and culture conditions; and cutting and managing the explant. The potamogeton malaianus is cultured in Hoagland nutrient solution, the explant is induced to sprout by gibberellins and generate a new root system by indolebutyric acid, and then the potamogeton malaianus explant with induced root and sprout is cut into a seedling culture field and cultivated into a finished product of seedlings. A large number of potamogeton malaianus seedlings can be propagated in a short time period; and the method is easy to operate, effectively avoids the phenomenon that cut seedlings do not root, improves the survival rate of the cut seedlings and meets the engineering requirements because a large number of seedlings can be propagated in a short time period.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of propagation method of submerged plant p.malaianus.
Background technology
P.malaianus (Potamogeton malaianus Miq.) Potamogetonaceae (Potamogetonaceae), Potamogeton (Potamogeton).Perennial heavy water floats the leaf draft, and there is fibrous root in joint portion, and the sprout on it upwards develops into plant, has flourishing underground root-like stock system; Grow in the harder lake of geology, pond and the river course.Remove minorities such as Shanxi, Gansu, Ningxia in China and economize especially, all there is distribution all the other each province.P.malaianus all once was the advantage kind in many lakes, and still along with the aggravation of body eutrophication, p.malaianus fades away, and rivers and lakes have been difficult to find p.malaianus now.Recover aquatic ecosystem, reconstruction p.malaianus population is extremely urgent.The p.malaianus seedling is main at present at present gathers by the natural river lake, is difficult to satisfy the large-scale engineering needs, and the seedling problem has become the bottleneck problem of aquatic ecosystem reparation and structure.
The cottage propagation technology is one of method the most frequently used in the plant propagation process.In the water plants breeding, also often be used.P.malaianus is the important component part of aquatic ecosystem.Recover the p.malaianus population and can effectively reduce water body eutrophication degree, improve the diversity of aquatic ecosystem, therefore p.malaianus is that aquatic vegetation recovers and body eutrophication is administered the conventional plant kind of planting in the engineering, but breeding, p.malaianus utilization cottage method has certain difficulty, the direct cuttage of explant can't grow root system for a long time, can't survive.Therefore want to make the cuttage explant to survive, the root system that its growth is made new advances is very crucial.
Summary of the invention
Goal of the invention: the objective of the invention is provides a kind of propagation method of p.malaianus in order to overcome the deficiencies in the prior art, and this method can effectively solve the phenomenon that cuttage seeding is not taken root, and improves the survival rate of cuttage seeding.
Technical scheme: in order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of propagation method of p.malaianus comprises the steps:
(1) from natural water, gathers the bright body of p.malaianus that well-grown does not have damage by disease and insect, fall the bright surface of p.malaianus attachment with flushing with clean water;
(2) will cut into the explant of 12 ~ 15 centimeter length behind the p.malaianus stem section removal leaf, each explant comprises 2 ~ 3 internodes;
(3) described explant is put into contained plant hormone Huo Gelan nutrient solution and cultivate, the control temperature was cultivated 10 ~ 12 days between 15 ℃ ~ 25 ℃;
(4) explant after will cultivating with flushing with clean water after, cuttage is in the field of growing seedlings;
(5) dark 0.3 meter of the water in field that keeps growing seedlings was cultivated 30 ~ 40 days, surpassed when the p.malaianus root system is long that 2 centimetres, plant are long transplants to the target water body during above 30 centimetres.
Contain 1 ~ 5 milligram of gibberellin, 1 ~ 4 milligram of indolebutyric acid in every liter of described nutrient solution of Glan suddenly.
In the step (3), the cultivation of described explant also needs to carry out illumination 4000～5000 luxs, and daytime, ratio was 1: 1.
In the step (5), also need to control water temperature in the cultivating process between 15 ℃～25 ℃.
Principle of the present invention is: promote the growth of root and bud in Huo Gelan (Hoagland) nutrient solution by hormone induction.Wherein gibberellin can stimulate the growth of leaf and bud; Indolebutyric acid can be induced the formation of root substance, promotes the formation of cutting adventive root, breeds seedling by cuttage then.
Beneficial effect: compared with prior art, advantage of the present invention is simple to operate, can breed a large amount of p.malaianus seedlings in a short time; Can effectively solve the phenomenon that cuttage seeding is not taken root, improve the survival rate of cuttage seeding, and can breed a large amount of seedlings in a short time, satisfy requirement of engineering.
Embodiment
The present invention is further described below by specific embodiment; should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the principle of the invention; can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Embodiment 1: the propagation method of a kind of p.malaianus of the present invention comprises the steps:
1, gathers the p.malaianus that well-grown does not have damage by disease and insect, fall the p.malaianus surface attachments with flushing with clean water;
2, p.malaianus stem section is sheared, every section keeps 2～3 internodes, long 12 centimetres, removes leaf;
3, the condition of culture of explant: shear good p.malaianus and put into and contain plant hormone Huo Gelan nutrient solution and cultivate, temperature remains on 25 ℃, illumination 4000～5000LUX, and daytime, ratio was 1: 1, cultivated to grow new root and bud in 10 days;
Wherein every liter of Huo Gelan (Hoagland) nutrient solution also contains 1 milligram of gibberellin except containing Huo Gelan nutrient solution basic element, 1 milligram of indolebutyric acid;
The method of 1 liter of Huo Gelan nutrient solution of preparation is as follows:
(1) preparation iron salt solutions
The prescription of iron salt solutions is:
Ferrous sulfate heptahydrate 2.78g
Disodium ethylene diamine tetraacetate (EDTA.Na) 3.73g
Adding distil water is settled to 500ml;
(2) preparation trace element solution
The prescription of trace element solution is:
Potassium iodide 0.83mg
Boric acid 6.2mg
Manganese sulphate 22.3mg
Zinc sulphate 8.6mg
Sodium molybdate 0.25mg
Copper sulphate 0.025mg
Cobalt chloride 0.025mg
Adding distil water is settled to 1000ml;
(3) iron salt solutions (1) 2.5ml is drawn in the preparation of Huo Gelan nutrient solution, and liquid microelement (2) 5ml adds distilled water 500ml, adds following chemicals then:
Four water-calcium nitrate 945mg
Potassium nitrate 506mg
Ammonium nitrate 80mg
Potassium dihydrogen phosphate 136mg
Magnesium sulfate 493mg
(4) be settled to 1 liter with distilled water, adjusting pH at last is 6.0;
(5) contain to step (4) and add 1 milligram of gibberellin, 1 milligram of indolebutyric acid in the Hoagland solution of basic element.
When 4, treating that the root of explant and blastogenesis are grown to 1 centimetre, with its with cuttage after the flushing with clean water 1 time to the field of growing seedlings;
5, dark 0.3 meter of the water in field that keeps growing seedlings, 15 ℃～25 ℃ of water temperatures were cultivated 30 days, treated to carry out when plant grows to 30 centimetres the transplanting of target water body.
Can obtain a large amount of p.malaianus seedlings by implementing said method, it is transplanted to the depth of water is that survival rate is 95% in certain man-made lake of 2.5 meters.
Embodiment 2: a kind of p.malaianus propagation method, and its concrete implementation step is as follows:
1, in the river, gathers the p.malaianus that the well-grown that comes does not have damage by disease and insect, can remove the p.malaianus surface attachments for 3 times with flushing with clean water and fall.
2, p.malaianus stem section is cut into the explant of 15 centimeter length, every section keeps 2～3 internodes, removes leaf.
3, will shear good p.malaianus explant puts into and contains plant hormone Huo Gelan (Hoagland) nutrient solution and cultivate.Keep 25 ℃ of temperature, control intensity of illumination and time, to cultivate 10 days, its root system and blastogenesis are about 1 centimeter length.
Wherein the compound method of Huo Gelan nutrient solution is substantially the same manner as Example 1, and different is the addition of gibberellin and indolebutyric acid, 5 milligrams of gibberellin, 4 milligrams of indolebutyric acids;
4, with after the p.malaianus explant usefulness flushing with clean water 1 time, cuttage is in the field of growing seedlings.
5, dark 0.3 meter of the water in field that keeps growing seedlings was cultivated 40 days, and long 30 centimetres of the long 2 centimetres of plant of root carry out the target water body and transplant.
Breed a large amount of p.malaianus seedlings by said method, it is transplanted to the depth of water is that survival rate is 93% in certain man-made lake of 2.5 meters.
Embodiment 3: the propagation method of a kind of p.malaianus of the present invention comprises the steps:
1, gathers the p.malaianus that well-grown does not have damage by disease and insect, use flushing with clean water 2～3 times, remove its surface attachments;
2, p.malaianus stem section is sheared, every section keeps 2～3 internodes, is about 12～15 centimetres, removes leaf;
3, the condition of culture of explant: the p.malaianus that shearing is good is put into the Huo Gelan nutrient solution and is cultivated, and temperature remains on 25 ℃, illumination 4000～5000LUX, and daytime, ratio was 1: 1, cultivated to grow new root and bud in 12 days;
Wherein the compound method of Huo Gelan nutrient solution is substantially the same manner as Example 1, and different is the addition of gibberellin and indolebutyric acid, 3 milligrams of gibberellin, 2 milligrams of indolebutyric acids;
When 4, treating that the root of explant and blastogenesis are grown to 1 centimetre, with its with cuttage after the flushing with clean water 1 time to the field of growing seedlings;
5, dark 0.3 meter of the water in field that keeps growing seedlings, 15 ℃～25 ℃ of water temperatures were cultivated 35 days, treated to carry out when plant grows to 30 centimetres the transplanting of target water body.
Can obtain a large amount of p.malaianus seedlings by implementing said method, it is transplanted to the depth of water is that survival rate is 97% in certain man-made lake of 2.5 meters.
Claims (4)
1. the propagation method of a p.malaianus, it is characterized in that, comprises the steps:
(1) from natural water, gathers the bright body of p.malaianus that well-grown does not have damage by disease and insect, fall the bright surface of p.malaianus attachment with flushing with clean water;
(2) will cut into the explant of 12 ~ 15 centimeter length behind the p.malaianus stem section removal leaf, each explant comprises 2 ~ 3 internodes;
(3) described explant is put into contained plant hormone Huo Gelan nutrient solution and cultivate, the control temperature was cultivated 10 ~ 12 days between 15 ℃ ~ 25 ℃;
(4) explant after will cultivating with flushing with clean water after, cuttage is in the field of growing seedlings;
(5) dark 0.3 meter of the water in field that keeps growing seedlings was cultivated 30 ~ 40 days, surpassed when the p.malaianus root system is long that 2 centimetres, plant are long transplants to the target water body during above 30 centimetres.
2. the propagation method of a kind of p.malaianus according to claim 1 is characterized in that, contains 1 ~ 5 milligram of gibberellin, 1 ~ 4 milligram of indolebutyric acid in every liter of described nutrient solution of Glan suddenly.
3. the propagation method of a kind of p.malaianus according to claim 1 is characterized in that, in the step (3), the cultivation of described explant also needs to carry out illumination 4000～5000 luxs, and daytime, ratio was 1: 1.
4. the propagation method of a kind of p.malaianus according to claim 1 is characterized in that, in the step (5), also needs to control water temperature in the cultivating process between 15 ℃～25 ℃.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102654929A CN101946620B (en) | 2010-08-27 | 2010-08-27 | Method for propagating potamogeton malaianus |
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| CN2010102654929A CN101946620B (en) | 2010-08-27 | 2010-08-27 | Method for propagating potamogeton malaianus |
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| CN101946620A true CN101946620A (en) | 2011-01-19 |
| CN101946620B CN101946620B (en) | 2011-12-07 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493871A (en) * | 2016-01-06 | 2016-04-20 | 纪效峰 | Cultivation method of camphor leaf grass |
| CN105941157A (en) * | 2016-06-13 | 2016-09-21 | 中国科学院亚热带农业生态研究所 | Potamogeton malainus single-stem-fragment fast propagation method |
| CN106857206A (en) * | 2017-01-12 | 2017-06-20 | 浙江省林业科学研究院 | A kind of D. primulinum Hydroponic culture and cultural method |
| CN109258428A (en) * | 2018-11-06 | 2019-01-25 | 桂林理工大学 | The p.malaianus seedling fast breeding method of artificial mattress |
| CN109496828A (en) * | 2018-11-27 | 2019-03-22 | 南京大学 | A method of promoting floating heart root growth |
| CN109601156A (en) * | 2019-01-16 | 2019-04-12 | 中国水产科学研究院珠江水产研究所 | A kind of comb tooth bog pondweed cuttage propagation method |
| CN111374033A (en) * | 2020-04-22 | 2020-07-07 | 广西壮族自治区中国科学院广西植物研究所 | Water planting cutting seedling method for giant reed |
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2010
- 2010-08-27 CN CN2010102654929A patent/CN101946620B/en active Active
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| CN105493871A (en) * | 2016-01-06 | 2016-04-20 | 纪效峰 | Cultivation method of camphor leaf grass |
| CN105941157A (en) * | 2016-06-13 | 2016-09-21 | 中国科学院亚热带农业生态研究所 | Potamogeton malainus single-stem-fragment fast propagation method |
| CN105941157B (en) * | 2016-06-13 | 2018-02-02 | 中国科学院亚热带农业生态研究所 | A kind of quick propagation method of p.malaianus Single stem segment |
| CN106857206A (en) * | 2017-01-12 | 2017-06-20 | 浙江省林业科学研究院 | A kind of D. primulinum Hydroponic culture and cultural method |
| CN109258428A (en) * | 2018-11-06 | 2019-01-25 | 桂林理工大学 | The p.malaianus seedling fast breeding method of artificial mattress |
| CN109496828A (en) * | 2018-11-27 | 2019-03-22 | 南京大学 | A method of promoting floating heart root growth |
| CN109601156A (en) * | 2019-01-16 | 2019-04-12 | 中国水产科学研究院珠江水产研究所 | A kind of comb tooth bog pondweed cuttage propagation method |
| CN111374033A (en) * | 2020-04-22 | 2020-07-07 | 广西壮族自治区中国科学院广西植物研究所 | Water planting cutting seedling method for giant reed |
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|---|---|
| CN101946620B (en) | 2011-12-07 |
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