CN109496828A - A method of promoting floating heart root growth - Google Patents
A method of promoting floating heart root growth Download PDFInfo
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- CN109496828A CN109496828A CN201811424646.7A CN201811424646A CN109496828A CN 109496828 A CN109496828 A CN 109496828A CN 201811424646 A CN201811424646 A CN 201811424646A CN 109496828 A CN109496828 A CN 109496828A
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- Prior art keywords
- floating heart
- fresh body
- root
- heart
- floating
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- 241000245301 Nymphoides Species 0.000 title claims abstract description 127
- 235000000476 Nymphoides peltata Nutrition 0.000 title claims abstract description 125
- 230000002786 root growth Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000001737 promoting effect Effects 0.000 title claims abstract description 16
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000002028 Biomass Substances 0.000 claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 21
- 235000015097 nutrients Nutrition 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 238000010521 absorption reaction Methods 0.000 claims abstract description 7
- 238000005286 illumination Methods 0.000 claims description 29
- 239000000243 solution Substances 0.000 claims description 27
- 239000012153 distilled water Substances 0.000 claims description 22
- 239000002689 soil Substances 0.000 claims description 15
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 229960002523 mercuric chloride Drugs 0.000 claims description 9
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 9
- 241000607479 Yersinia pestis Species 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 7
- 238000011010 flushing procedure Methods 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 7
- 239000008239 natural water Substances 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 6
- 150000002505 iron Chemical class 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 239000004323 potassium nitrate Substances 0.000 claims description 6
- 235000010333 potassium nitrate Nutrition 0.000 claims description 6
- 239000012266 salt solution Substances 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 4
- 239000004327 boric acid Substances 0.000 claims description 3
- 125000005619 boric acid group Chemical group 0.000 claims description 3
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 3
- BXRRQHBNBXJZBQ-UHFFFAOYSA-L dichloromanganese;hydrate Chemical compound O.Cl[Mn]Cl BXRRQHBNBXJZBQ-UHFFFAOYSA-L 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims description 2
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- NASFKTWZWDYFER-UHFFFAOYSA-N sodium;hydrate Chemical compound O.[Na] NASFKTWZWDYFER-UHFFFAOYSA-N 0.000 claims description 2
- 238000012549 training Methods 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 229960001484 edetic acid Drugs 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical class S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 12
- 230000012010 growth Effects 0.000 abstract description 11
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229930192334 Auxin Natural products 0.000 description 7
- 239000002363 auxin Substances 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 239000004745 nonwoven fabric Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 3
- 239000012496 blank sample Substances 0.000 description 3
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 3
- 238000012851 eutrophication Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000023560 heart growth Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016768 molybdenum Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The invention discloses a kind of methods for promoting floating heart root growth, it is the following steps are included: the selection and disinfection treatment of the fresh body of floating heart, the configuration of culture solution and the selection of condition of culture, the addition of growth-promoting root agent etc.；By treated, the fresh body of floating heart is put into the domestication culture of Huo Gelan nutrient solution, and the biomass carbon using absorption heteroauxin promotes floating heart root system sturdy.Operation of the present invention is simple, can promote the fresh body well developed root system of floating heart within a short period of time；It can effectively solve the fresh body of floating heart and the phenomenon that slow growth, improve the survival rate of the fresh body of floating heart in river up to 83.3%, meet water ecology reparation and the practical needs planted of floating heart.
Description
Technical field
The invention belongs to field of environmental biotechnology, and in particular to a kind of method for promoting floating heart root growth.
Background technique
Floating heart (Nymphoides peltatum) is the raw floatingleaved plant of root common in shallow lakes in the middle and lower reaches of the Yangtze Rive, is
Long Dan section nymphoides peltatum belongs to perennial aquatic and herbaceous, annual early spring, walks stem in subterranean stem (Su Sheng) pumping water, born on stipes it is several not
Determine root, and grows blade.Florescence is annual August in May-, and fruiting period is August-October.It is grown on pond, lake, irrigation canals and ditches, rice field
In the steady waters in equal hydrostatic and river or river mouth, usual all living creatures, in single advantage group.Floating heart group is the middle and lower reach of Yangtze River
More floatingleaved plant group is distributed in water body, but as water eutrophication is aggravated, floating heart growth is restricted, and root system rots
Seriously, survival rate is low, therefore restores aquatic ecosystem, and it is extremely urgent to rebuild floating heart population.
Auxin is a kind of plant hormone found earliest, is served in plant growth and development process highly important.
On organ level, auxin affects growth, maturation and the aging of organ；On a cellular level, auxin affects cell
Elongation, division and differentiation.Auxin is to the regulating and controlling effect of plant growth and development, because of its concentration, floristics, organ and cell
Age it is different and different, root growth and development is closely related with auxin.Floating heart is the important component of aquatic ecosystem, is promoted
Into the growth of floating heart root system, water body eutrophication degree can be effectively reduced, and then improve the diversity of aquatic ecosystem, therefore
Floating heart is the conventional plant kind in macrophyte planting and water eutrophication harnessing project, and water plant root system is aquatic
The key of plant growth, therefore promote water plant root growth particularly important using auxin.
Summary of the invention
The purpose of the present invention is limited for current floating heart growing environment, root system rots serious problem, provides one kind
Promote the method for floating heart root growth.
The present invention is achieved by the following technical solutions:
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 2-3 times with clear water, removes its surface
Attachment；
(2) root disinfection, disinfection will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart
It is clean with distilled water flushing afterwards, it is then placed in distilled water and impregnates；
(3) by step (2), treated that the fresh body of floating heart is put into Huo Gelan nutrient solution, is mutually tied using illumination cultivation with dark culture
The mode of conjunction is cultivated；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, heteroauxin is taken to be adsorbed in biomass carbon,
And the biomass carbon for completing absorption is in the root system embedment soil of the fresh body of floating heart；
(5) it was measured every 2-3 days and observes the fresh body root growth situation of floating heart.
The technical solution that the present invention further solves is that the disinfecting time in the step (2) in mercuric chloride solution is
10min；The time impregnated in merging distilled water is for 24 hours.
The technical solution that the present invention further solves is the preparation of Huo Gelan nutrient solution in the step (3) are as follows: every 1000
In the distilled water of mL, the potassium nitrate of 0.25mL, the bitter salt of 0.1mL, the four water-calcium nitrate of 0.25mL, 0.15mL are added
Iron salt solutions, the ammonium chloride of 0.1mL, the potassium dihydrogen phosphate of 0.1mL and 1mL liquid microelement；Wherein, the potassium nitrate
Concentration be 50.5mg/mL, the concentration of the bitter salt is 49.3mg/mL, and the concentration of the four water-calcium nitrate is
118mg/mL, the concentration of the ammonium chloride are 5.349mg/mL, and the concentration of the potassium dihydrogen phosphate is 6.9mg/mL.
The technical solution that the present invention further solves is the preparation of the iron salt solutions are as follows: the distilled water of every 1000 mL
In, add the iron sulfate heptahydrate of 27.85g and the disodium ethylene diamine tetraacetate of 37.25g.
The technical solution that the present invention further solves is that the liquid microelement is boric acid, four chloride hydrate manganese, seven hydrations
Zinc sulfate, Salzburg vitriol, two molybdic acid hydrate sodium are matched according to mass ratio 2.86: 1.81: 0.22: 0.08: 0.12
System.
The technical solution that the present invention further solves is the condition of training method in the step (3) are as follows: the illumination
Light source is fluorescent lamp, and the time ratio for controlling illumination cultivation and dark culture is 1:1, and incubation time is 7 days；Wherein, day temperature control
It is made as 30-35 DEG C, night temperatures control is 25-30 DEG C, day light hours 12h, intensity of illumination 2000Lx.
The end scheme that the present invention further solves is, in the step (4), the absorption of biomass carbon and heteroauxin is closed
System are as follows: the heteroauxin of every 3g biomass carbon absorption 100mL；Wherein, the concentration of the heteroauxin is 5-50 μm of ol/L.Tool
During gymnastics is made, the biomass carbon for having adsorbed heteroauxin can be fitted into the sack of non-woven fabrics and charcoal bag is made, and by charcoal
The root system surrounded around the fresh body of floating heart is embedded to soil.
The technical solution that the present invention further solves is, in the step (5), the fresh body root growth situation of floating heart includes:
The total root long of the fresh body of floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart.
The invention has the benefit that
A kind of method promoting floating heart root growth of the present invention is put after carrying out explant disinfection to the fresh body root system of floating heart
Enter in Huo Gelan nutrient solution and cultivated in such a way that illumination cultivation is combined with dark culture, while being adsorbed using biomass carbon
Auxin heteroauxin, and be distributed in around the fresh body root system of floating heart, to promote the fast-growth of the fresh body root system of floating heart.This hair
It is bright easy to operate, the growth of floating heart root system can be promoted within a short period of time, effectively solve floating heart slow growth in river
Phenomenon improves the survival rate of floating heart up to 83.3%, meets the practical needs planted of floating heart.
Detailed description of the invention
Fig. 1 is the relational graph of different heteroauxin concentration and the fresh body survival rate of floating heart of the invention.
Fig. 2 is that present invention root long total under various concentration heteroauxin changes over time curve graph.
Fig. 3 is that the present invention total root surface area of the fresh body of floating heart under various concentration heteroauxin changes over time curve graph.
Fig. 4 is that the present invention total root volume of the fresh body of floating heart under various concentration heteroauxin changes over time curve graph.
Specific embodiment
Summary of the invention of the invention is further described with reference to the accompanying drawings and examples.
Embodiment 1
Configure Huo Gelan nutrient solution, take in the distilled water of 1000mL, add the potassium nitrate of 0.25mL, the magnesium sulfate of 0.1mL,
The four water-calcium nitrate of 0.25mL, the iron salt solutions of 0.15mL, the ammonium chloride of 0.1mL, the potassium dihydrogen phosphate of 0.1mL and 1mL
Liquid microelement.Wherein, the concentration of the potassium nitrate is 50.5mg/L, and the concentration of the bitter salt is 49.3mg/L,
The concentration of the four water-calcium nitrate is 118mg/L, and the concentration of the ammonium chloride is 5.349mg/L, the potassium dihydrogen phosphate it is dense
Degree is 6.9mg/L.
The iron salt solutions are the mixed solution of iron sulfate heptahydrate and disodium ethylene diamine tetraacetate, concrete configuration are as follows: take
The distilled water of 1000mL weighs the iron sulfate heptahydrate of 27.85 g and the disodium ethylene diamine tetraacetate of 37.25 g, and distillation is added
It mixes and stands in water.
The liquid microelement is boric acid, four chloride hydrate manganese, Zinc vitriol, Salzburg vitriol, two hydration molybdenums
Sour sodium is prepared to obtain according to mass ratio 2.86: 1.81: 0.22: 0.08: 0.12.
Embodiment 2
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 2 times with clear water, it is attached to remove its surface
Object；
(2) root will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart sterilize 10min,
It is clean with distilled water flushing after disinfection, it is then placed in distilled water and impregnates for 24 hours；
(3) by the Huo Gelan nutrient solution of step (2) treated the fresh body of floating heart the is put into configuration of embodiment 1, using illumination cultivation
The mode combined with dark culture is cultivated；The light source of the illumination is fluorescent lamp, control illumination cultivation and dark culture when
Between than be 1:1, incubation time be 7 days；Wherein, day temperature control is 30 DEG C, and night temperatures control is 25 DEG C, when daylight
Between be 12h, intensity of illumination 2000Lx；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, the heteroauxin of 5 μm of ol/L of 100mL is taken to inhale
It invests in the biomass carbon of 3g, then biomass carbon is fitted into non-woven fabrics sack, the root system around the fresh body of floating heart is embedded to together
In soil；
(5) it every the total root long of 3 days fresh bodies of measurement floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart, and observes
The survival rate of the fresh body of floating heart.
Embodiment 3
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 3 times with clear water, it is attached to remove its surface
Object；
(2) root will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart sterilize 10min,
It is clean with distilled water flushing after disinfection, it is then placed in distilled water and impregnates for 24 hours；
(3) by the Huo Gelan nutrient solution of step (2) treated the fresh body of floating heart the is put into configuration of embodiment 1, using illumination cultivation
The mode combined with dark culture is cultivated；The light source of the illumination is fluorescent lamp, control illumination cultivation and dark culture when
Between than be 1:1, incubation time be 7 days；Wherein, day temperature control is 32 DEG C, and night temperatures control is 28 DEG C, when daylight
Between be 12h, intensity of illumination 2000Lx；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, takes the heteroauxin of 10 μm of ol/L of 100mL
It is adsorbed in the biomass carbon of 3g, then biomass carbon is fitted into non-woven fabrics sack, the root system around the fresh body of floating heart buries together
Enter in soil；
(5) it every the total root long of 3 days fresh bodies of measurement floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart, and observes
The survival rate of the fresh body of floating heart.
Embodiment 4
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 3 times with clear water, it is attached to remove its surface
Object；
(2) root will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart sterilize 10min,
It is clean with distilled water flushing after disinfection, it is then placed in distilled water and impregnates for 24 hours；
(3) by the Huo Gelan nutrient solution of step (2) treated the fresh body of floating heart the is put into configuration of embodiment 1, using illumination cultivation
The mode combined with dark culture is cultivated；The light source of the illumination is fluorescent lamp, control illumination cultivation and dark culture when
Between than be 1:1, incubation time be 7 days；Wherein, day temperature control is 30 DEG C, and night temperatures control is 28 DEG C, when daylight
Between be 12h, intensity of illumination 2000Lx；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, takes the heteroauxin of 20 μm of ol/L of 100mL
It is adsorbed in the biomass carbon of 3g, then biomass carbon is fitted into non-woven fabrics sack, the root system around the fresh body of floating heart buries together
Enter in soil；
(5) it every the total root long of 3 days fresh bodies of measurement floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart, and observes
The survival rate of the fresh body of floating heart.
Embodiment 5
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 2 times with clear water, it is attached to remove its surface
Object；
(2) root will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart sterilize 10min,
It is clean with distilled water flushing after disinfection, it is then placed in distilled water and impregnates for 24 hours；
(3) by the Huo Gelan nutrient solution of step (2) treated the fresh body of floating heart the is put into configuration of embodiment 1, using illumination cultivation
The mode combined with dark culture is cultivated；The light source of the illumination is fluorescent lamp, control illumination cultivation and dark culture when
Between than be 1:1, incubation time be 7 days；Wherein, day temperature control is 32 DEG C, and night temperatures control is 30 DEG C, when daylight
Between be 12h, intensity of illumination 2000Lx；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, takes the heteroauxin of 40 μm of ol/L of 100mL
It is adsorbed in the biomass carbon of 3g, then biomass carbon is fitted into non-woven fabrics sack, the root system around the fresh body of floating heart buries together
Enter in soil；
(5) it every the total root long of 3 days fresh bodies of measurement floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart, and observes
The survival rate of the fresh body of floating heart.
Embodiment 6
A method of promoting floating heart root growth, includes the following steps:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 2 times with clear water, it is attached to remove its surface
Object；
(2) root will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart sterilize 10min,
It is clean with distilled water flushing after disinfection, it is then placed in distilled water and impregnates for 24 hours；
(3) by the Huo Gelan nutrient solution of step (2) treated the fresh body of floating heart the is put into configuration of embodiment 1, using illumination cultivation
The mode combined with dark culture is cultivated；The light source of the illumination is fluorescent lamp, control illumination cultivation and dark culture when
Between than be 1:1, incubation time be 7 days；Wherein, day temperature control is 35 DEG C, and night temperatures control is 30 DEG C, when daylight
Between be 12h, intensity of illumination 2000Lx；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, takes the heteroauxin of 40 μm of ol/L of 100mL
It is adsorbed in the biomass carbon of 3g, then biomass carbon is fitted into non-woven fabrics sack, the root system around the fresh body of floating heart buries together
Enter in soil；
(5) it every the total root long of 3 days fresh bodies of measurement floating heart, the total root surface area of the fresh body of floating heart and the total root volume of the fresh body of floating heart, and observes
The survival rate of the fresh body of floating heart.
Fig. 1 is embodiment 2 into embodiment 6, and the influence using various concentration heteroauxin to the fresh body survival rate of floating heart is closed
System's figure, as shown in figure, compared with the blank sample for being not added with heteroauxin, the fresh body survival rate of floating heart of the embodiment 2 into embodiment 6
Reach 83.3%, higher than 50% survival rate for being not added with heteroauxin, thus illustrate heteroauxin can be improved floating heart at
Motility rate.
Fig. 2 is embodiment 2 into embodiment 6, and the total root long of the fresh body of floating heart changes over time under various concentration heteroauxin
Curve graph, as shown in figure, the total root long growth rate of the fresh body of floating heart for adding heteroauxin is higher than the blank sample for being not added with heteroauxin；
With the growth of time, the total root long of the fresh body of floating heart is gradually increased, and as the concentration of heteroauxin increases, total root long rate of rise
Gradually accelerate；When heteroauxin concentration is greater than 40 μm of ol/L, the stabilization of advancing the speed of the total root long of the fresh body of floating heart.
Fig. 3 is embodiment 2 into embodiment 6, and the total root surface area of the fresh body of floating heart is at any time under various concentration heteroauxin
Change curve, as shown in figure, the total root surface area growth rate of the fresh body of floating heart for adding heteroauxin, which is higher than, is not added with heteroauxin
Blank sample；With the growth of time, the total root surface area of the fresh body of floating heart is gradually increased, and as the concentration of heteroauxin increases,
Total root surface area rate of rise is accelerated；When heteroauxin concentration is greater than 40 μm of ol/L, the increase of the total root surface area of the fresh body of floating heart
Rate stabilization.
Fig. 4 is embodiment 2 into embodiment 6, and the total root volume of the fresh body of floating heart becomes at any time under various concentration heteroauxin
Change curve graph, as shown in figure, the total root volume growth rate of water plant for adding heteroauxin is higher than the sky for being not added with heteroauxin
White sample；With the growth of time, the total root volume of the fresh body of floating heart is gradually increased, and as the concentration of heteroauxin increases, total root body
Product rate of rise is accelerated；When heteroauxin concentration is greater than 20 μm of ol/L, the stabilization of advancing the speed of the total root volume of the fresh body of floating heart.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention
Protection scope.
Claims (8)
1. a kind of method for promoting floating heart root growth, which comprises the steps of:
(1) the fresh body of floating heart that well-grown no disease and pests harm is acquired from natural water, is rinsed 2-3 times with clear water, removes its surface
Attachment；
(2) root disinfection, disinfection will be carried out in the mercuric chloride solution of the fresh body merging 0.1wt% of step (1) pretreated floating heart
It is clean with distilled water flushing afterwards, it is then placed in distilled water and impregnates；
(3) by step (2), treated that the fresh body of floating heart is put into Huo Gelan nutrient solution, is mutually tied using illumination cultivation with dark culture
The mode of conjunction is cultivated；
(4) the fresh body of floating heart after step (3) domestication culture is cultivated into soil, heteroauxin is taken to be adsorbed in biomass carbon,
And the biomass carbon for completing absorption is in the root system embedment soil of the fresh body of floating heart；
(5) it was measured every 2-3 days and observes the fresh body root growth situation of floating heart.
2. a kind of method for promoting floating heart root growth according to claim 1, which is characterized in that in the step (2)
Disinfecting time in mercuric chloride solution is 10min；The time impregnated in merging distilled water is for 24 hours.
3. a kind of method for promoting floating heart root growth according to claim 1, which is characterized in that in the step (3)
The preparation of Huo Gelan nutrient solution are as follows: in the distilled water of every 1000 mL, add the potassium nitrate of 0.25mL, seven hydrated sulfuric acids of 0.1mL
Magnesium, the four water-calcium nitrate of 0.25mL, the iron salt solutions of 0.15mL, the ammonium chloride of 0.1mL, 0.1mL potassium dihydrogen phosphate and 1mL
Liquid microelement；Wherein, the concentration of the potassium nitrate is 50.5mg/mL, and the concentration of the bitter salt is 49.3mg/
ML, the concentration of the four water-calcium nitrate are 118mg/mL, and the concentration of the ammonium chloride is 5.349mg/mL, the potassium dihydrogen phosphate
Concentration be 6.9mg/mL.
4. a kind of method for promoting floating heart root growth according to claim 3, which is characterized in that the iron salt solutions
It prepares are as follows: in the distilled water of every 1000 mL, add the iron sulfate heptahydrate of 27.85g and the ethylenediamine tetra-acetic acid two of 37.25g
Sodium.
5. a kind of method for promoting floating heart root growth according to claim 3, which is characterized in that the liquid microelement
It is boric acid, four chloride hydrate manganese, Zinc vitriol, Salzburg vitriol, two molybdic acid hydrate sodium according to mass ratio 2.86:
Prepared at 1.81: 0.22: 0.08: 0.12.
6. the method for a kind of promotion floating heart root growth according to claim 1, which is characterized in that the step
(3) condition of training method in are as follows: the light source of the illumination is fluorescent lamp, and the time ratio for controlling illumination cultivation and dark culture is 1:
1, incubation time is 7 days；Wherein, day temperature control is 30-35 DEG C, and night temperatures control is 25-30 DEG C, day light hours
For 12h, intensity of illumination 2000Lx.
7. the method for a kind of promotion floating heart root growth according to claim 1, which is characterized in that the step
(4) in, the absorption relationship of biomass carbon and heteroauxin are as follows: the heteroauxin of every 3g biomass carbon absorption 100mL；Wherein, institute
The concentration for stating heteroauxin is 5-50 μm of ol/L.
8. the method for a kind of promotion floating heart root growth according to claim 1, which is characterized in that the step
(5) in, the fresh body root growth situation of floating heart includes: that the total root long of the fresh body of floating heart, the total root surface area of the fresh body of floating heart and the fresh body of floating heart are total
Root volume.
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