CN109258428A - The p.malaianus seedling fast breeding method of artificial mattress - Google Patents

The p.malaianus seedling fast breeding method of artificial mattress Download PDF

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Publication number
CN109258428A
CN109258428A CN201811309899.XA CN201811309899A CN109258428A CN 109258428 A CN109258428 A CN 109258428A CN 201811309899 A CN201811309899 A CN 201811309899A CN 109258428 A CN109258428 A CN 109258428A
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China
Prior art keywords
malaianus
water
stem
seedling
days
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CN201811309899.XA
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Chinese (zh)
Inventor
张琴
刘辉利
李金城
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Guilin University of Technology
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Guilin University of Technology
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Priority to CN201811309899.XA priority Critical patent/CN109258428A/en
Publication of CN109258428A publication Critical patent/CN109258428A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/327Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants

Abstract

The present invention relates to a kind of breeding methods of p.malaianus seedling for the artificial mattress of pollution waters restoration.The present invention uses p.malaianus stem section, using polluted-water as nutrient solution, it is pre-processed using exogenous hormone, lead to the supply that oxygen technology guarantees p.malaianus stem section dissolved oxygen and nutrition by deep liquid stream water culture technique and interval, induce the new root and branch for adapting to pollution water environment, achieve the purpose that cultivate the p.malaianus seedling for being used for the artificial mattress of pollution waters restoration, is cultivated for domestication and provide new method for the p.malaianus of pollution waters restoration.

Description

The p.malaianus seedling fast breeding method of artificial mattress
Technical field
The present invention relates to a kind of bog pondweed seedling fostering method, specifically a kind of artificial mattress for pollution waters restoration P.malaianus seedling fast breeding method.
Technical background
Artificial mattress is widely used in the restoration of the ecosystem of eutrophication water, and obtains good clean-up effect, heavy water Plant plays a crucial role wherein.Submerged plant in artificial mattress can be to nitrogen, the phosphorus and not degradable in water body and bed mud Organic pollutant absorbed, converted, and Self substances are synthesized, to play catharsis to eutrophication water.Meanwhile It can also provide living space for microorganism and generate depression effect to the growth of algae and breeding to a certain extent, to rise Effect in terms of improvement water ecological environment.
P.malaianus (Potamogeton malaianus), perennial aquatic and herbaceous, rhizome is flourishing, white, diameter 1.5-2 millimeters, multi-branched often in formation fusiform suspend mode sprout, and has slightly close fibrous root at section, is suitable for hydrostatic, moves Aquatic length can survive under the conditions of different waters and substrate, be polluted-water artificial mattress building common submerged plant it One.Under natural conditions, the p.malaianus mainly more hibernaculum breeding by being grown on fruit, rhizomes and rhizomes;When fruit at It is scattered and is floated in water after ripe, propagate fruit when by means of paddy field irrigation and drainage;Nutrient growth early period by the bud on rhizomes, develops into new root Shape stem and the cauline leaf on ground.In addition, tradition or open tissue culture mode also have the seedling training applied to p.malaianus It educates.Currently, technical problem common in production has: easy to pollute, inoculation hardly possible, take root hardly possible, acclimatization and transplants survival rate are low etc..Due to dirt The usual complicated component of water body is contaminated, containing heavy metal, pathogenic microorganism and other poisonous and harmful elements, therefore it is good using root system development It is good, there is the seedling of preferable adaptability to water quality, is conducive to successfully construct the artificial mattress for pollution waters restoration.Between The measures such as oxygenation, exogenous hormone pretreatment of having a rest can promote the formation of p.malaianus root system and the generation of shoot, improve seedling Vigor and the survival rate of transplanting.
Summary of the invention
The object of the present invention is to provide the breeding methods of the p.malaianus seedling of the artificial mattress of water remediation, for quickly training It educates and new method is provided for the p.malaianus of water remediation.
The present invention is to combine deep flow regime to cultivate Malaysia using interval oxygenation+exogenous hormone pretreatment in polluted-water Bog pondweed seedling.The method of the present invention can induce the new root for adapting to pollution water environment to p.malaianus and generate new point Branch achievees the purpose that cultivate the p.malaianus seedling for being used for water remediation, particular content are as follows:
1. acquiring the fresh body of p.malaianus of well-grown no disease and pests harm from natural water, Malaysia eye is rinsed out with clear water The sub- fresh body surface attachments of dish;It is characterized in that used p.malaianus is the fresh body of well-grown no disease and pests harm.
2. cutting into the stem section of 10-15 centimeter length after taking the upright stem section removal leaf of p.malaianus, each stem section is at least wrapped Containing 2-3 complete internodes;It is characterized in that the p.malaianus stem section taken is upright stem section, the length sheared is 10- 15 centimetres, each stem section includes 2-3 complete internodes.
3. p.malaianus stem section is all immersed in configured preprocessing solution (containing heteroauxin (IBA) 10mg/L+ 6- benzyl purine (6-BA) 10mg/L+ gibberellin (GA3) 20mg/L+ kinetin (KT) 5mg/L) in, after carrying out immersion treatment 3h It takes out, is put into progress water planting root system induction in dedicated hydroponic device.It is characterized in that the exogenous hormone be heteroauxin, 6- benzyl purine, gibberellin and kinetin, the concentration of processing are respectively 10mg/L, 10mg/L, 20mg/L and 5mg/L, processing side Formula is directly to impregnate stem section, handling duration 3h.
4. preparing culture substrate: taking diameter is the quartz sand of 0.5-1mm, and tap water is cleaned, drained;Located in advance with exogenous hormone Manage the solution (benzyl purine Han heteroauxin (IBA) 10mg/L+6- (6-BA) 10mg/L+ gibberellin (GA3) 20mg/L+ kinetin (KT) 5mg/L) in, immersion treatment 12h is carried out, then drain, it is spare;It is characterized in that culture substrate is the warp of diameter 0.5-1mm Drained and standby quartz sand after exogenous hormone preprocessing solution impregnates 12 hours, exogenous hormone preprocessing solution contain heteroauxin (IBA) 10mg/L+6- benzyl purine (6-BA) 10mg/L+ gibberellin (GA3) 20mg/L+ kinetin (KT) 5mg/L;
5. preparing culture vessel: bottom has the basket or sieve in hole and culture substrate is layed in culture after interior bottom is laid with filter membrane Vessel bottom, with a thickness of 3-4cm;Culture vessel is set up in nursery slot, is kept at a distance from 1-2cm with nursery trench bottom.It is special Sign is that the laying depth of quartz sand is 3-4cm, and culture vessel bottom has hole, is set up in nursery slot, apart with nursery trench bottom 1-2cm。
6. cultivating initial stage (1-10 days), by pretreated p.malaianus stem section forward direction cuttage to nursery tank culture matrix In, insertion depth 0.8-1.5cm;It is cultivated using deep flow regime, keeps sluggish flow flowing, control depth of water 20- 30cm, floods p.malaianus stem section in water by 20-25 DEG C of water temperature;Keep illumination sufficient;Lead to oxygen using every 4h simultaneously The mode of 30min carries out interval and leads to oxygen, and disposes occur rotten stem section in Induction Process in time;It is characterized in that domestication training The technology educated is deep liquid stream water culture technique, sluggish flow, depth of water 20-30cm, 20-25 DEG C of water temperature, hydraulic detention time 2 days, often Lead to oxygen 30min every 4h.
7. cultivating mid-term (11-20 days), p.malaianus stem section adapts to water body, and new root is born on section, and generates new point Branch, gradually reduces the frequency of oxygenation, leads to oxygen to carry out interval in such a way that 8h leads to oxygen 30min.It is characterized in that using deep liquid Stream mode is cultivated, sluggish flow, depth of water 20-30cm, 20-25 DEG C of water temperature, hydraulic detention time 2 days, leads to oxygen 30min every 8h.
8. cultivating later period (21-30 days), long 1-2 branch in p.malaianus stem section, take root on stipes and new branch, root Long 8-10cm.Adjustment interval oxygenation mode is to lead to oxygen 30min every 12h, until plant is taken out from matrix, is transplanted in nature Water body.It is characterized in that cultivated using deep flow regime, sluggish flow, depth of water 20-30cm, 20-25 DEG C of water temperature, when hydraulic retention Between 2 days, lead to oxygen 30min every 12h.
Compared with prior art, the invention has the characteristics that:
1. avoiding the waste and pollution of nutriment using polluted-water as culture solution, p.malaianus seedling pair is improved The adaptability of polluted-water.
2. exogenous hormone preconditioning technique, deep liquid stream water culture technique and interval are led to oxygen technology to be organically combined, lead to Exogenous hormone pretreatment culture substrate and p.malaianus stem section are crossed, when extending exogenous hormone and the effect of p.malaianus stem section Between, induction p.malaianus generates new root and branch.It is molten that guarantee is combined by deep liquid stream water culture technique and the logical oxygen technology of interval The supply of oxygen and nutrition, it is ensured that the success of p.malaianus nursery.
3. cultivating process is carried out sublevel segment management, not only reduced energy consumption but also the p.malaianus seedling for cultivating completion is made more Polluted-water environment is adapted to, cultivating successful seedling can directly transplant to artificial mattress, reduce hardening and adapt to link.
Specific embodiment
Embodiment:
Lead to oxygen technology using exogenous hormone pretreatment, deep liquid stream water culture technique and interval in polluted-water to combine, it is right P.malaianus stem section induction culturing obtains the p.malaianus seedling for adapting to pollution water environment, specific steps are as follows:
(1) from the fresh body of p.malaianus of acquisition well-grown no disease and pests harm, the fresh body of p.malaianus is rinsed out with clear water Surface attachments, cut into the stem section of 10-15 centimeter length after disleaf, each stem section includes at least 2-3 complete internodes, there are It is spare to 50 p.malaianus stem sections.
(2) taking diameter is the quartz sand of 0.5-1mm, and tap water is cleaned, drained, spare.
(3) it prepares and contains the benzyl purine Han heteroauxin (IBA) 10mg/L+6- (6-BA) 10mg/L+ gibberellin (GA3) P.malaianus stem section obtained by (1) is all immersed in by the exogenous hormone preprocessing solution of 20mg/L+ kinetin (KT) 5mg/L Wherein, it is taken out after keeping 3h;It is drained and standby after quartz sand obtained by (2) is impregnated 12 hours in exogenous hormone preprocessing solution.
(4) the rectangle porous plastics basket for preparing 1 30 × 50cm is used as culture vessel, and bottom elder generation is with 60 mesh thin,tough silk in basket It is rebasing, then (3) resulting quartz sand is laid with thereon, with a thickness of 3-4cm.
(5) by p.malaianus stem section forward direction cuttage obtained by (3) in the culture substrate prepared through (4), insertion depth is 0.8-1.5cm;It again by entire culture vessel, is set up in the nursery slot of special hydroponic device, with nursery trench bottom at a distance of 1- 2cm keeps water flow unimpeded.
(6) start hydroponic device, keep sluggish flow flowing, control depth of water 20-30cm, 20-25 DEG C of water temperature, make Malaysia eye Sub- dish stem section is flooded in water;Keep illumination sufficient;While leading to oxygen using carrying out interval in such a way that 4h leads to oxygen 30min; The 10th day cultivated, all stem sections take root on section and generate new branch, average root long 0.5cm.Period is disposed in time Occur rotten stem section in cultivating process, avoids that lesion occurs.
(7) it after cultivating 11 days, keeps other conditions constant, reduces the frequency of oxygenation, in such a way that 8h leads to oxygen 30min It carries out interval and leads to oxygen;To the 20th day of cultivation, more root is grown on p.malaianus section, average root is long to be greater than 3cm, each Stem section grows 1-2 branch, and 1-2 piece leaf is grown on new branch.Period, which still needs to dispose in time, there is rotten stem section, avoids Lesion occurs.
(8) after cultivating 21 days, adjustment interval oxygenation mode is to lead to oxygen 30min every 12h;To induction culturing the 30th day, 50 A p.malaianus stem section grows aquatic root system abundant, and average root long is more than 5cm, and each stem section grows 1-2 new points Branch, and grow 3-4 piece leaf.
(9) it by 50 plants of p.malaianus spriggings to natural pollution water body, all survives, without dead strain phenomenon.

Claims (4)

1. a kind of breeding method of the p.malaianus seedling of the artificial mattress for pollution waters restoration, which is characterized in that choosing It is cultivated with the fresh body stem section of p.malaianus into polluted-water, cultivates and obtained within 25-30 days having adaptation polluted-water to repair Multiple p.malaianus seedling leads to the measures such as oxygen, exogenous hormone pretreatment using interval in cultivating process and promotes new root and branch Generation.
2. the cultivation side of the p.malaianus seedling of the artificial mattress according to claim 1 for pollution waters restoration Method, which is characterized in that the fresh body stem section of the p.malaianus is that the fresh p.malaianus of nature growth is cut after going disleaf The stem section of 10-15 centimeter length made of cutting, culture solution is polluted-water.
3. the breeding method of the p.malaianus seedling of the artificial mattress according to claim 1 for pollution waters restoration Exogenous hormone preprocess method, which is characterized in that the exogenous hormone pretreatment fluid includes that heteroauxin, 6- benzyl are fast Purine, gibberellin and kinetin, the concentration of processing are respectively 10mg/L, 10mg/L, 20mg/L and 5mg/L;The object of processing is horse Come bog pondweed stem section and and its culture substrate;Processing mode is directly to impregnate, and wherein the handling duration of stem section is 3h, culture substrate Handling duration be 12h.
4. the cultivation side of the p.malaianus seedling of the artificial mattress according to claim 1 for pollution waters restoration Method, the cultivation initial stage (1-10 days), it is characterised in that tame the technology of cultivation as deep liquid stream water culture technique, depth of water 20- 30cm, hydraulic detention time 2 days, leads to oxygen 30min every 4h by 20-25 DEG C of water temperature;It cultivates mid-term (11-20 days), it is characterised in that Deep flow regime is cultivated, and sluggish flow, depth of water 20-30cm, hydraulic detention time 2 days, leads to oxygen every 8h by 20-25 DEG C of water temperature 30min;It cultivates later period (21-30 days), it is characterised in that cultivated using deep flow regime, sluggish flow, depth of water 20-30cm, water Warm 20-25 DEG C, hydraulic detention time 2 days, lead to oxygen 30min every 12h.
CN201811309899.XA 2018-11-06 2018-11-06 The p.malaianus seedling fast breeding method of artificial mattress Pending CN109258428A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1982235A (en) * 2005-11-03 2007-06-20 中国科学院水生生物研究所 Method for purifying biological settled bed water quality
CN101946620A (en) * 2010-08-27 2011-01-19 南京中科水治理工程有限公司 Method for propagating potamogeton malaianus
CN205011476U (en) * 2015-08-28 2016-02-03 上海太和水环境科技发展有限公司 Ecological preliminary treatment system for riverway water body is restoreed
CN107646652A (en) * 2017-10-30 2018-02-02 桂林理工大学 The root system induction method of water planting bracketplant is repaired for black and odorous water
CN108101328A (en) * 2018-01-26 2018-06-01 中冶华天工程技术有限公司 The method that heavy metal in bottom mud in lake is removed using submerged plant

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1982235A (en) * 2005-11-03 2007-06-20 中国科学院水生生物研究所 Method for purifying biological settled bed water quality
CN101946620A (en) * 2010-08-27 2011-01-19 南京中科水治理工程有限公司 Method for propagating potamogeton malaianus
CN205011476U (en) * 2015-08-28 2016-02-03 上海太和水环境科技发展有限公司 Ecological preliminary treatment system for riverway water body is restoreed
CN107646652A (en) * 2017-10-30 2018-02-02 桂林理工大学 The root system induction method of water planting bracketplant is repaired for black and odorous water
CN108101328A (en) * 2018-01-26 2018-06-01 中冶华天工程技术有限公司 The method that heavy metal in bottom mud in lake is removed using submerged plant

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