CN104094843A - Ceratophyllum demersum breeding method and application - Google Patents
Ceratophyllum demersum breeding method and application Download PDFInfo
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- CN104094843A CN104094843A CN201410207616.6A CN201410207616A CN104094843A CN 104094843 A CN104094843 A CN 104094843A CN 201410207616 A CN201410207616 A CN 201410207616A CN 104094843 A CN104094843 A CN 104094843A
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Abstract
The invention discloses a ceratophyllum demersum culture method, and the method is as follows: cutting ceratophyllum demersum stem segments to the length of 5 to 6 cm for indoor culture in Hoagland culture liquid, and inducing lateral bud growth under the condition of addition of 0.1 to 2.0mg / L of gibberellic acid. 20-25 days after the indoor culture, the length of the stem segments is 15-20 cm, the stem segments are transplanted into a seedling field for cultivation for 25 to 30 days, and then are planted into target water, the planting survival rate reaches 95% above, and the method has the important meaning in the construction and the stability of the lake ecosystem.
Description
Technical field
The invention belongs to biological technical field, be specifically related to the hormone induction propagation method of a kind of submerged plant hornwort.
Background technology
Hornwort (
ceratophyllum demersuml.) be Ceratophyllaceae (
ceratophyliaceae) goldfish Trentepohlia (
ceratophylluml.) a kind of submerged plant.Aquatic herbaceous plant for many years, unrooted, stem is floating, and branch is flourishing.Hornwort perennation is in small lakes hydrostatic place, once in pond, ditch etc., locates commonly, and all there is distribution all parts of the country, nowadays almost can't see wild population.Hornwort profile is graceful, is general types in the lakebed greening of view lake and aquarium.In small-sized lake treatment and ecosystem structure, hornwort is also indispensable plant variety, because its application is comparatively extensive and demand is larger.
Hornwort in the fall illumination is gradually short, and during mercury dropped, side shoot top stops growing, the intensive one-tenth of leaf leafage, and look deepens green, and cutin thickens, and accumulates the nutrients such as starch, becomes a kind of special nutrition propagule, dormancy terminal bud.Now plant becomes fragile, and terminal bud very easily comes off, and is sunken to dormancy in mud and survives the winter, and Second Year is sprouted for new strain spring.Hornwort unrooted, complete stool submerged in the water, thereby growth and illumination in close relations, when water is too muddy, in water, penetrate light less, hornwort growth is bad, but when water penetrates sunlight clearly after, still can recover to grow.Hornwort requires wider to water temperature, but comparatively responsive to freezing, and in ice, in several days, freezes to death.Hornwort is nitrophile, and in water, the high growth of content of inorganic nitrogen in better.
The hormone induction breeding of hornwort, has solved this difficult problem of demand deficiency, by making full use of, bring into play the Landscape Application value of hornwort and the pioneer in water pollution control engineering, is worth.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide that a kind of survival rate is high, the propagation method of the submerged plant hornwort that can shorten repoductive time, because hornwort does not have root system, so only need to induce the growth of its stem in incubation.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: the propagation method of a kind of hornwort, the method comprises the steps:
(1) collection growth conditions is good, without the hornwort plant of damage by disease and insect;
(2) hornwort plant is cleaned 2 ~ 3 times with clear water, and impurity is wherein removed totally, obtain explant;
(3) clean explant is cut into the length of 5 ~ 6 centimetres;
(4) explant of having sheared is put into Huo Gelan (
hoagland) cultivate in culture fluid; In room temperature, be 25 ℃ of cultivations, round the clock than being 1:1, cultivate 20 ~ 25 days;
(5) during collateral generation to 15 ~ 20 centimetre, it is taken out from culture fluid, with clear water, rinse 1 time; Plantation is cultivated to the Tanaka that grows seedlings;
(6) the Tanaka that grows seedlings, keep depth of water 25-35 centimetre, 15 ~ 30 ℃ of water temperatures, cultivate 25 ~ 30 days, when plant grows to 30 centimetres, can be used as kind of a transplantation of seedlings.
In step (4), Huo Gelan (
hoagland) culture fluid comprises: Huo Gelan (
hoagland) basic element, gibberellin 0.1 ~ 2.0mg/L.
Utilize the seedling that said method is cultivated out to recover the application in engineering in water pollution control and aquatic vegetation.
Utilize the application in the greening of view lake lakebed and water scenery landscape engineering of seedling that said method cultivates out.
Beneficial effect: adopt propagation method of the present invention, make the survival rate of hornwort more than 95%, within its vegetative period, good to the clean-up effect of various form nitrogen (especially nitrate nitrogen), phosphorus in water, hornwort is to the structure of lake ecosystem and stablize significant.
embodiment:
Below by specific embodiment, the present invention is described in further detail.
Embodiment 1: the propagation method of a kind of hornwort, method comprises the steps:
1, collection growth conditions is good, without the hornwort plant of damage by disease and insect;
2, hornwort plant is cleaned 3 times with clear water, and impurity is wherein removed totally, obtain explant;
3, clean explant is cut into the length of 5 ~ 6 centimetres;
4, the explant of having sheared is put into Huo Gelan (
hoagland) cultivate in culture fluid; In room temperature, be 25 ℃ of cultivations, round the clock than being 1:1, cultivate 20 days;
Wherein, 1 liter of Huo Gelan of preparation (
hoagland) method of culture fluid is as follows:
(1) preparation iron salt solutions
The formula of iron salt solutions is:
Ferrous sulfate heptahydrate 2.78g;
Disodium ethylene diamine tetraacetate (EDTA.Na) 3.73g;
Adding distil water is settled to 500ml.
(2) preparation trace element solution
The formula of trace element solution is:
Potassium iodide 0.83mg, boric acid 6.2mg, manganese sulphate 22.3mg, zinc sulphate 8.6mg, sodium molybdate 0.25mg, copper sulphate 0.025mg, cobalt chloride 0.025mg;
Adding distil water is settled to 1000ml.
(3) preparation of Huo Gelan nutrient solution, draws iron salt solutions (1) 2.5ml, and liquid microelement (2) 5ml, adds distilled water 500ml, then adds following chemicals:
Four water-calcium nitrate 945mg, potassium nitrate 506mg, ammonium nitrate 80mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 493mg.(4) with distilled water, be settled to 1 liter, finally adjusting pH is 6.0;
(5) Huo Gelan (
hoagland) add gibberellin 0.1mg/L in basic element.
5, during collateral generation to 15 centimetre, it is taken out from culture fluid, with clear water, rinse 1 time; Plantation is cultivated to the Tanaka that grows seedlings;
6, the Tanaka that grows seedlings, keep 30 centimetres of the depth of waters, 15 ~ 30 ℃ of water temperatures, cultivate 25 days, when plant grows to 30 centimetres, can be used as kind of a transplantation of seedlings.
By said method, cultivate hornwort 30000 strains, be applied to the man-made lake aquatic vegetation planting engineering in certain city.2 meters of mean depth of water, survival rate reaches 98%.Hornwort strain shape is graceful, configures other submerged plant growth, forms natural water ecology view.In hornwort application in earlier stage, water body total nitrogen content 4.8mg/L, total phosphorus content 0.58mg/L application hornwort is after 40 days, and water body total nitrogen content is down to 1.9mg/L, and reduction rate reaches 60%; Water body total phosphorus content is down to 0.2 mg/L, and reduction rate reaches 65%, and more than local water transparency reaches 2m.
Embodiment 2: the propagation method of a kind of hornwort, method comprises the steps:
1, collection growth conditions is good, without the hornwort plant of damage by disease and insect;
2, hornwort plant is cleaned 2 times with clear water, and impurity is wherein removed totally, obtain explant;
3, clean explant is cut into the length of 5 ~ 6 centimetres;
4, the explant of having sheared is put into Huo Gelan (
hoagland) cultivate in culture fluid; In room temperature, be 25 ℃ of cultivations, round the clock than being 1:1, cultivate 25 days; Wherein Huo Gelan (
hoagland) culture fluid is substantially the same manner as Example 1, difference is that gibberellin is 2.0mg/L.
5, during collateral generation to 20 centimetre, it is taken out from culture fluid, with clear water, rinse 1 time; Plantation is cultivated to the Tanaka that grows seedlings;
6, the Tanaka that grows seedlings, keep 25 centimetres of the depth of waters, 15 ~ 30 ℃ of water temperatures, cultivate 30 days, when plant grows to 30 centimetres, can be used as kind of a transplantation of seedlings.
By said method, cultivate hornwort 35000 strains, planted in the aquatic vegetation planting engineering in natural lake, 2.8 meters of lake mean depth of water, survival rate reaches 96%, the hornwort surviving in water body, configure other in, background pasture and water, form the water landscape that level is graceful, come in every shape.In hornwort application in earlier stage, water body total nitrogen content 4.3mg/L, total phosphorus content 0.82 mg/L application hornwort is after 40 days, and water body total nitrogen content is down to 1.8mg/L, and reduction rate reaches 58%; Water body total phosphorus content is down to 0.4 mg/L, and reduction rate reaches 51%, and more than local water transparency reaches 3m.
Embodiment 3: the propagation method of a kind of hornwort, method comprises the steps:
(1) collection growth conditions is good, without the hornwort plant of damage by disease and insect;
(2) hornwort plant is cleaned 3 times with clear water, and impurity is wherein removed totally, obtain explant;
(3) clean explant is cut into the length of 5 ~ 6 centimetres;
(4) explant of having sheared is put into Huo Gelan (
hoagland) cultivate in culture fluid; In room temperature, be 25 ℃ of cultivations, round the clock than being 1:1, cultivate 22 days; Wherein Huo Gelan (
hoagland) culture fluid is substantially the same manner as Example 1, difference is that gibberellin is 1.2mg/L.
(5) during collateral generation to 18 centimetre, it is taken out from culture fluid, with clear water, rinse 1 time; Plantation is cultivated to the Tanaka that grows seedlings;
(6) the Tanaka that grows seedlings, keep 28 centimetres of the depth of waters, 15 ~ 30 ℃ of water temperatures, cultivate 26 days, when plant grows to 30 centimetres, can be used as kind of a transplantation of seedlings.
By said method, cultivate hornwort 50000 strains, be applied in waters, somewhere, Taihu Lake, survival rate reaches 97%.The hornwort surviving configures other pasture and water such as eel grass, watermifoil in water body, forms the water landscape of nature grace.Hornwort is applied early stage, water body total nitrogen content 5.6mg/L, total phosphorus content 0.61mg/L, application hornwort is after 40 days, and water body total nitrogen content is down to 2.7mg/L, and reduction rate is 51%, total phosphorus content is down to 0.4 mg/L, and reduction rate is 34%, and promotes more than water transparency reaches 3m.So hornwort is to the structure of lake ecosystem and stablize significant.
Claims (4)
1. a propagation method for hornwort, is characterized in that, the method comprises the steps:
(1) collection growth conditions is good, without the hornwort plant of damage by disease and insect;
(2) hornwort plant is cleaned 2 ~ 3 times with clear water, and impurity is wherein removed totally, obtain explant;
(3) clean explant is cut into the length of 5 ~ 6 centimetres;
(4) explant of having sheared is put into Huo Gelan (
hoagland) cultivate in culture fluid; In room temperature, be 25 ℃ of cultivations, round the clock than being 1:1, cultivate 20 ~ 25 days;
(5) during collateral generation to 15 ~ 20 centimetre, it is taken out from culture fluid, with clear water, rinse 1 time; Plantation is cultivated to the Tanaka that grows seedlings;
(6) the Tanaka that grows seedlings, keep depth of water 25-35 centimetre, 15 ~ 30 ℃ of water temperatures, cultivate 25 ~ 30 days, when plant grows to 30 centimetres, can be used as kind of a transplantation of seedlings.
2. the propagation method of hornwort according to claim 1, is characterized in that, Huo Gelan (
hoagland) culture fluid comprises: Huo Gelan (
hoagland) basic element, gibberellin 0.1 ~ 2.0mg/L.
3. utilize the seedling that claim 1 method cultivates out to recover the application in engineering in water pollution control and aquatic vegetation.
4. utilize the application in the greening of view lake lakebed and water scenery landscape engineering of seedling that claim 1 method cultivates out.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184557A (en) * | 2017-12-19 | 2018-06-22 | 深圳市铁汉生态环境股份有限公司 | A kind of propagation method of the climing algae in river |
| CN109984040A (en) * | 2019-04-15 | 2019-07-09 | 北京林业大学 | A kind of the stem section quick propagation method and equipment of hornwort |
| CN115812533A (en) * | 2022-11-21 | 2023-03-21 | 中国科学院武汉植物园 | Method for rapidly recovering bitter thorn population |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184557A (en) * | 2017-12-19 | 2018-06-22 | 深圳市铁汉生态环境股份有限公司 | A kind of propagation method of the climing algae in river |
| CN109984040A (en) * | 2019-04-15 | 2019-07-09 | 北京林业大学 | A kind of the stem section quick propagation method and equipment of hornwort |
| CN109984040B (en) * | 2019-04-15 | 2020-12-18 | 北京林业大学 | Rapid stem propagation method for Goldfish algae |
| CN115812533A (en) * | 2022-11-21 | 2023-03-21 | 中国科学院武汉植物园 | Method for rapidly recovering bitter thorn population |
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