CN110651702A

CN110651702A - Indoor cultivation method of salicornia europaea

Info

Publication number: CN110651702A
Application number: CN201911112315.4A
Authority: CN
Inventors: 段慧荣; 杨红善; 周学辉; 崔光欣; 路远; 田福平; 胡宇; 朱新强
Original assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Current assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority date: 2019-11-14
Filing date: 2019-11-14
Publication date: 2020-01-07

Abstract

The invention provides a method for indoor cultivation of salicornia europaea, and belongs to the technical field of plant planting. A method for indoor cultivation of salicornia europaea comprises the following steps: soaking the cleaned salicornia europaea seeds for 2-3 h, and then carrying out germination culture to obtain seedlings; transplanting the seedlings into a plug tray filled with a fine sand matrix, and exposing the matrix at the top ends of the seedlings for seedling culture; and (3) starting to irrigate 1/2Hoagland nutrient solution containing 50-200 mmol/LNaCl for seedlings with the age of 1-4 weeks, and irrigating the nutrient solution once every 2 days. The culture method provided by the invention determines the optimal growth environment of salicornia europaea, can obtain salicornia europaea seedlings which grow rapidly and well, and provides a more convenient and efficient way for further development and utilization.

Description

Indoor cultivation method of salicornia europaea

Technical Field

The invention belongs to the technical field of plant planting, and particularly relates to a method for indoor cultivation of salicornia europaea.

Background

Drought and salinization are two major abiotic stresses that limit crop growth and agricultural production worldwide. In an agricultural ecosystem, the two stresses often occur together, and particularly in arid and semiarid regions, secondary salinization caused by unreasonable irrigation is particularly serious. The drought or halophyte plants are widely distributed in arid and semiarid regions, and gradually form a special adaptive mechanism in the long-term evolution process, so that the method has important application value in the aspect of genetic improvement of crops and fine pasture.

Salicornia europaea (Salicornia europaea) is the dominant species of saline-alkali land in the northwest of China, and is the halophyte which has been reported to be most salt-tolerant. In long-term adaptation to salinization extreme environments, salicornia leaf is degenerated into scaly, and developed stem fleshy tissue is formed to prevent water loss in plants and increase the ionization regionalization capacity. The salicornia europaea can absorb a large amount of salt in a habitat into a body and regionalize the salicornia europaea in a vacuole on the overground part to maintain normal growth, and the important salt-tolerant gene donor can be widely applied to salt-tolerant genetic engineering improvement work of various crops and ecological engineering plants and comprehensive treatment of saline-alkali soil. Has very important practical significance for the development and the utilization of salicornia europaea and has wide development prospect.

Presently, Salicornia bigelovii (Salicornia bigelovii), a closely related species of Salicornia Salicornia, has been commercially popularized on a large scale. However, the wild salicornia europaea resources in China have the problems of poor seed quality, low germination rate, slow growth and the like, so crop production cannot be carried out, large-scale artificial planting is not available, and the indoor culture method is not perfect.

Disclosure of Invention

In view of the above, the present invention aims to provide a method for indoor cultivation of salicornia europaea, which determines the optimal growth environment of salicornia europaea, can obtain salicornia europaea seedlings with rapid growth and good growth vigor, and provides a more convenient and efficient way for further development and utilization.

The invention provides a method for indoor cultivation of salicornia europaea, which comprises the following steps:

1) soaking the cleaned salicornia europaea seeds for 2-3 h, and performing germination culture to obtain seedlings; the temperature of the germination culture is 23-27 ℃, and the humidity is 55-65%; the germination culture is dark culture;

2) transplanting the seedlings into a plug tray filled with a fine sand matrix, exposing the matrix at the top ends of the seedlings, and culturing the seedlings;

the day temperature of seedling culture is 23-27 ℃, and the night temperature is 20-24 ℃; the relative humidity of the environment during seedling culture is 60-70%; the seedling culture is accompanied by illumination; the illumination time is 15-18 h/d; the illumination intensity is 18000-22000 Lux;

and (3) starting to irrigate 1/2Hoagland nutrient solution containing 50-200 mmol/L NaCl to seedlings with the age of 1-4 weeks, and irrigating the nutrient solution once every 2 days.

Preferably, the concentration of NaCl in the nutrient solution in the step 2) is 100 mmol/L.

Preferably, the 1/2Hoagland nutrient solution in the step 2) has the following formula: 2mmol/L KNO₃、0.5mmol/L KH₂PO₄、0.5mmol/L MgSO₄·7H₂O、0.5mmol/L Ca(NO₃)₂·4H₂O、50μmol/L H₃BO₃、10μmol/L MnCl₂·4H₂O、1.6μmol/L ZnSO₄·7H₂O、0.6μmol/L CuSO₄·5H₂O、0.05μmol/L Na₂MoO₄·2H₂O and 0.06mmol/L Fe-citrate-3H₂O。

Preferably, the number of seedlings transplanted in each hole of the hole tray in the step 2) is 1.

Preferably, the temperature of the seedling culture in the step 2) is 25 ℃ in the day and 22 ℃ in the night; the relative humidity of the environment when the seedlings are cultured is 65%.

Preferably, the illumination time in the step 2) is 16 h/d; the intensity of the illumination is 20000 Lux.

Preferably, the germination culture in step 1) is performed in a petri dish spread with two layers of wetted filter paper.

Preferably, the temperature of the germination cultivation in step 1) is 25 ℃ and the humidity is 60%.

Preferably, the salicornia europaea seeds in the step 1) are mature, full and golden in color.

The invention provides a method for indoor cultivation of salicornia europaea, the technical scheme of the invention is adopted to carry out indoor cultivation on salicornia europaea, compared with the seedlings of a control group which is not added with salt nutrient solution, the seedlings grow rapidly, are robust, have high fleshy degree, greatly shorten the growth time of the seedlings and are not interfered by the external environment. Therefore, the invention provides an efficient indoor culture method of salicornia europaea, determines the optimal growth environment of the salicornia europaea, can obtain salicornia europaea seedlings which grow rapidly and grow well, and provides a more convenient and efficient way for further development and utilization. Meanwhile, the method provided by the invention is simple and feasible, and can provide an important technical means for developing large-scale popularization of salicornia europaea.

Drawings

FIG. 1 is a graph showing the growth of Salicornia Herbacea after being irrigated with nutrient solutions containing NaCl at different concentrations for 7 days;

FIG. 2 is a dry weight column diagram of the roots and overground parts of Salicornia europaea after irrigation with a nutrient solution containing NaCl at different concentrations for 7 d;

FIG. 3 is a graph showing the degree of granulation of Salicornia europaea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 7 days;

FIG. 4 is a graph showing the growth of Salicornia Herbacea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 60 days;

FIG. 5 is a dry weight column graph of Salicornia Herbacea roots and aerial parts after being irrigated with nutrient solutions containing NaCl at different concentrations for 60 days;

FIG. 6 is a graph showing the degree of granulation of Salicornia europaea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 60 days.

Detailed Description

the day temperature of seedling culture is 23-27 ℃, and the night temperature is 20-24 ℃; the relative humidity of the environment during seedling culture is 60-70%; the seedling culture is accompanied by illumination; the illumination time is 16 h/d; the illumination intensity is 18000-22000 Lux;

The method comprises the steps of soaking cleaned salicornia europaea seeds for 2-3 hours, and then carrying out germination culture to obtain seedlings; the temperature of the germination culture is 23-27 ℃, and the humidity is 55-65%; the germination culture is dark culture.

In the invention, the salicornia europaea seeds are preferably mature, full and golden in color. The washing is preferably carried out with distilled water; the number of washing is preferably 3 to 5, and more preferably 4. The temperature of the germination culture is preferably 25 ℃ and the humidity is preferably 60%. The germination culture is preferably performed in a petri dish plated with two layers of wetted filter paper. The culture dish and the filter paper are not particularly limited and can be made of conventional materials, such as a glass culture dish with a diameter of 9cm and common qualitative filter paper with a diameter of 9 cm. And (3) germinating seedlings are obtained when the germination culture is carried out for 3 d.

After obtaining the seedlings, transplanting the seedlings into a plug tray filled with a fine sand matrix, exposing the matrix at the top ends of the seedlings, and culturing the seedlings.

In the present invention, the concentration of NaCl in the nutrient solution is preferably 100 mmol/L. The 1/2Hoagland nutrient solution preferably has the following formula: 2mmol/L KNO₃、0.5mmol/L KH₂PO₄、0.5mmol/L MgSO₄·7H₂O、0.5mmol/L Ca(NO₃)₂·4H₂O、50μmol/L H₃BO₃、10μmol/LMnCl₂·4H₂O、1.6μmol/LZnSO₄·7H₂O、0.6μmol/L CuSO₄·5H₂O、0.05μmol/LNa₂MoO₄·2H₂O and 0.06 mmol/LFe-citrate.3H₂And O. The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.

The number of seedlings and trees transplanted in each hole in the hole tray is preferably 1. The day temperature of the seedling culture is preferably 25 ℃, and the night temperature is preferably 22 ℃; the relative humidity of the environment when the seedlings are cultured is preferably 65%. The illumination time is preferably 16 h/d; the intensity of the illumination is preferably 20000 Lux. The plug was washed every 3 days. The invention is not limited to the kind and specification of the tray, and the tray conventionally used in the art can be used, for example, a black plastic tray (5cm × 5cm × 5 cm).

The method for indoor cultivation of Salicornia europaea according to the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.

Example 1

1) Selecting completely mature Salicornia europaea seeds with plump seeds and golden color, washing with distilled water for 3 times, soaking in distilled water for 2 hr, placing the seeds in a culture dish (diameter of 9cm) with two layers of filter paper, and soaking the filter paper in distilled water. The culture dish was placed in an incubator for constant temperature culture at 25 ℃, humidity 60%, no light, and distilled water was added every day to keep the filter paper moist. After 3 days of germination in a dark room, transplanting the seedlings with good growth vigor and consistent germination into a hole tray filled with sterilized fine sand, 1 plant per hole, putting the base parts of the seedlings into the fine sand during planting, exposing the fine sand at the top ends of the seedlings, and carefully operating to avoid breaking tender tissues. And (4) culturing the planted salicornia herbacea in an artificial climate box. The day and night temperature of the artificial climate box is 25 ℃/22 ℃, the illumination is 16h/d, the illumination intensity is about 20000Lux, and the relative humidity is 65%. 1/2Hoagland nutrient solution containing 50mM NaCl was poured 1 time every 2 days, the plastic trays were cleaned every 3 days, and the nutrient solution was replaced with new one.

Continuously irrigating 1/2Hoagland nutrient solution, photographing when the seedlings grow to 37 days (after the 4-week-old seedlings are continuously cultured for 7 days) and 90 days (after the 4-week-old seedlings are continuously cultured for 60 days) to record the growth condition of the seedlings, and determining the dry fresh weight and the fleshy degree of the roots and the overground parts of the Salicornia europaea seedlings.

Example 2

1) Selecting completely mature Salicornia europaea seeds with plump seeds and golden color, washing with distilled water for 4 times, soaking in distilled water for 2.5 hr, placing the seeds in a culture dish (diameter of 9cm) with two layers of filter paper, and soaking the filter paper in distilled water. The Petri dish was incubated in an incubator at a constant temperature of 27 ℃ and a humidity of 55% without light, and distilled water was added every day to keep the filter paper moist. After 3 days of germination in a dark room, transplanting the seedlings with good growth vigor and consistent germination into a hole tray filled with sterilized fine sand, 1 plant per hole, putting the base parts of the seedlings into the fine sand during planting, exposing the fine sand at the top ends of the seedlings, and carefully operating to avoid breaking tender tissues. And (4) culturing the planted salicornia herbacea in an artificial climate box. The day and night temperature of the artificial climate box is 25 ℃/20 ℃, the illumination is 16h/d, the illumination intensity is about 18000Lux, and the relative humidity is 70%. 1/2Hoagland nutrient solution containing 100mM NaCl was poured 1 time every 2 days, the plastic trays were cleaned every 3 days, and the nutrient solution was replaced with new one.

Example 3

1) Selecting completely mature Salicornia europaea seeds with plump seeds and golden color, washing with distilled water for 5 times, soaking in distilled water for 3 hr, placing the seeds in a culture dish (diameter of 9cm) with two layers of filter paper, and soaking the filter paper in distilled water. The culture dish was placed in an incubator for constant temperature culture at 23 ℃ with a humidity of 65% and without light, and distilled water was added every day to keep the filter paper moist. After 3 days of germination in a dark room, transplanting the seedlings with good growth vigor and consistent germination into a hole tray filled with sterilized fine sand, 1 plant per hole, putting the base parts of the seedlings into the fine sand during planting, exposing the fine sand at the top ends of the seedlings, and carefully operating to avoid breaking tender tissues. And (4) culturing the planted salicornia herbacea in an artificial climate box. The day and night temperature of the artificial climate box is 25 ℃/24 ℃, the illumination is 16h/d, the illumination intensity is 22000Lux, and the relative humidity is 60%. 1/2Hoagland nutrient solution containing 200mM NaCl was poured 1 time every 2 days, the plastic trays were cleaned every 3 days, and the nutrient solution was replaced with new one.

Comparative example 1

This comparative example differs from example 1 in that: the saline cornucopia seedling is watered with 1/2Hoagland nutrient solution to grow to 4 weeks of age, and then 1/2Hoagland nutrient solution is watered.

Comparative example 2

This comparative example differs from example 1 in that: the saline cornucopia seedling is watered with 1/2Hoagland nutrient solution, and after the seedlings grow to 4 weeks of age, the seedlings are watered with 1/2Hoagland nutrient solution containing 300mM NaCl.

Experimental example 4

In this example, the growth of the Salicornia europaea seedlings cultured in examples 1 to 3 and comparative examples 1 to 2 was continuously observed, the seedlings of 4 weeks were respectively irrigated with different concentrations of saline nutrient solutions, and after treatment for 7d and 60d, the growth conditions and physiological indexes were counted, and the results are as follows.

FIGS. 1 to 3 show the results of irrigating the seedling 7d with different nutrient solutions. FIG. 1 shows the growth of Salicornia Herbacea after being irrigated with nutrient solutions containing NaCl at different concentrations for 7 days. As can be seen from FIG. 1, after the seedlings of salicornia europaea are irrigated for 7 days by using the nutrient solution containing 50-200 mM NaCl, the seedlings of salicornia europaea grow strongly, the overground part and the root part grow well, and the seedlings irrigated by using the nutrient solution containing no NaCl or 300mM NaCl have relatively small plants and undeveloped roots.

FIG. 2 is a dry-fresh weight bar graph of Salicornia Herbacea roots and aerial parts after irrigation with nutrient solutions containing NaCl at different concentrations for 7 days. Compared with the seedlings irrigated with the nutrient solution containing no NaCl or 300mM NaCl, the seedlings irrigated with the nutrient solution containing 50-200 mM NaCl for 7 days have obvious advantages in dry and fresh weight average of roots and overground parts, and the fact that the seedlings irrigated with the nutrient solution containing 50-200 mM NaCl is beneficial to rapid growth of the seedlings is proved.

FIG. 3 is a graph showing the degree of granulation of Salicornia Herbacea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 7 days. As can be seen from FIG. 3, the degree of the seedling formation by irrigation with the NaCl-free and NaCl-containing nutrient solutions ranging from low to high was normally distributed, with the highest degree of the seedling formation by irrigation with the nutrient solution containing 100mM NaCl, followed by irrigation with the nutrient solutions containing 50 and 200mM NaCl, and the lowest degree of the seedling formation by irrigation with the nutrient solution containing no NaCl or the nutrient solution containing 300mM NaCl.

FIGS. 4 to 6 show the results of irrigating the seedlings with different nutrient solutions for 60 days. FIG. 4 is a graph showing the growth of Salicornia Herbacea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 60 days. After the seedlings of the salicornia europaea are irrigated for 60 days by using the nutrient solution containing 50-200 mM NaCl, the salicornia europaea seedlings grow strongly, the stem and leaf parts are green, and when the seedlings of the nutrient solution containing no NaCl or the nutrient solution containing 300mM NaCl are irrigated, the plants are relatively short and the stem and leaf parts are yellow.

FIG. 5 is a dry and fresh weight chart of the roots and overground parts of salicornia europaea after being irrigated with nutrient solutions containing NaCl in different concentrations for 60 days. Compared with the nutrient solution without NaCl, the dry and fresh weight of the saline cornstalk roots and the overground parts is obviously different 60 days after the seedlings are irrigated by the nutrient solution containing NaCl. FIG. 6 is a graph showing the degree of granulation of Salicornia europaea seedlings after being irrigated with nutrient solutions containing NaCl at different concentrations for 60 days. Compared with the nutrient solution without NaCl, the nutrient solution containing NaCl shows a remarkable difference in the fleshy degree 60 days after the seedlings are irrigated.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A method for indoor cultivation of salicornia europaea is characterized by comprising the following steps:

2. The method according to claim 1, wherein the concentration of NaCl in the nutrient solution in step 2) is 100 mmol/L.

3. The method as claimed in claim 1, wherein the formulation of the 1/2Hoagland nutrient solution in step 2) is: 2mmol/L KNO₃、0.5mmol/L KH₂PO₄、0.5mmol/L MgSO₄·7H₂O、0.5mmol/L Ca(NO₃)₂·4H₂O、50μmol/L H₃BO₃、10μmol/L MnCl₂·4H₂O、1.6μmol/L ZnSO₄·7H₂O、0.6μmol/L CuSO₄·5H₂O、0.05μmol/L Na₂MoO₄·2H₂O and 0.06mmol/L Fe-citrate-3H₂O。

4. The method according to claim 1, wherein 1 seedling tree is transplanted per hole in the plug in step 2).

5. The method according to claim 1, wherein the seedling culture in step 2) is carried out at a temperature of 25 ℃ during the day and at a temperature of 22 ℃ during the night; the relative humidity of the environment when the seedlings are cultured is 65%.

6. The method according to claim 1, wherein the illumination time in step 2) is 16 h/d; the intensity of the illumination is 20000 Lux.

7. The method according to claim 1, wherein the germination culturing in step 1) is performed in a petri dish spread with two layers of wetted filter paper.

8. The method according to claim 1, wherein the temperature of the germination cultivation in step 1) is 25 ℃ and the humidity is 60%.

9. The method according to any one of claims 1 to 8, wherein the salicornia europaea seeds in step 1) are mature, full and golden-colored salicornia europaea seeds.