CN106465675A - A kind of ciltivating process of arabidopsiss - Google Patents
A kind of ciltivating process of arabidopsiss Download PDFInfo
- Publication number
- CN106465675A CN106465675A CN201610787994.5A CN201610787994A CN106465675A CN 106465675 A CN106465675 A CN 106465675A CN 201610787994 A CN201610787994 A CN 201610787994A CN 106465675 A CN106465675 A CN 106465675A
- Authority
- CN
- China
- Prior art keywords
- arabidopsiss
- seedling
- liquid medium
- mixture layer
- illumination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a kind of ciltivating process of arabidopsiss, comprise the steps：Step one, arabidopsiss seed is put in the alcoholic solution of mass-volume concentration 60% 90% and soaks, process at 0～4 DEG C of temperature and dark condition, then be placed in the seedling cultivating into long more than the 1.2cm of root in solid medium；Step 2, seedling move in liquid medium culture, and in bottom one layer of Vermiculitum of laying of liquid medium and the first mixture layer of shell Calcium Carbonate, under conditions of 18～22 DEG C of temperature, humidity 60%～80%, illumination 2000～3000lux and 24h illumination, culture 10～15 days, obtains seedling；Step 3, liquid medium of replacing, low layer in this new liquid medium lays the second mixture layer of Vermiculitum and eggshell powder simultaneously, under conditions of 18～22 DEG C of temperature, humidity 60%～80%, illumination 2000～3000lux and 24h illumination, continue culture seedling 10～15 days；Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
Description
Technical field
The present invention relates to a kind of ciltivating process of arabidopsiss.
Background technology
Arabidopsises are in Angiospermae, Dicotyledoneae.Biennial herb, China Inner Mongol, Xinjiang, Shaanxi, Gansu,
The provinces and regions such as Tibet, Shandong, Jiangsu, Anhui, Hubei, Sichuan, Yunnan are all found.The advantage of arabidopsiss is that plant is little, knot is many.
The genome of arabidopsiss is currently known in Plant Genome minimum.Arabidopsiss are self-pollination plants, gene high homogenous,
Very high with chemical factors process mutation rate, it is readily available the deficiency of various metabolic functions.Due to there being these advantages above-mentioned, so
Arabidopsiss are by the good material of genetics research, are described as " fruit bat in plant " by scientist.Scientific research often needs
Arabidopsis thaliana Seedlings to be used or the seedling of different times, due to water planting arabidopsiss, to have quick, convenient, growth of seedling fast and young
Seedling growth course is forced the more low reason of degree, and experimenter generally tends to water planting arabidopsiss.But, with during culture
Between extend or culturing room's use time longer, occur that in culture fluid, miscellaneous bacteria is more, seriously polluted, so that having a strong impact on plan more
Southern mustard growth of seedling, has a strong impact on experiment progress.
Content of the invention
It is an object of the invention to solving at least the above and/or defect, and provide at least will be described later excellent
Point.
It is a still further object of the present invention to provide a kind of ciltivating process of arabidopsiss.
The present invention provide technical scheme be：
A kind of ciltivating process of arabidopsiss, comprises the steps：
Step one, arabidopsiss seed is put in the alcoholic solution of mass-volume concentration 60%-90% and soaks 10-30min,
Process 3～4 days at 0～4 DEG C of temperature and dark condition, then be placed in solid medium and cultivate into long more than the 1.2cm of root
Seedling；
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and at this
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of liquid medium, in 18～22 DEG C of temperature, humidity
60%～80%, under conditions of illumination 2000～3000lux and 24h illumination, cultivate 10～15 days, obtain seedling；
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell of this new liquid medium
Second mixture layer of powder, in 18～22 DEG C of temperature, humidity 60%～80%, illumination 2000～3000lux and 24h illumination
Under the conditions of, continue culture seedling 10～15 days；
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
Preferably, in the ciltivating process of described arabidopsiss, described solid medium is 1/2MS solid medium.
Preferably, in the ciltivating process of described arabidopsiss, described liquid medium comprises：1/5～1/3MS, 0.1-
1.0mg/L 6-BA, 0.05-0.5mg/L KT and 0.1-0.5mg/L IAA.
Preferably, in the ciltivating process of described arabidopsiss, the thickness of described first mixture layer is described liquid training
The 1/5～1/3 of nutrient solution depth.
Preferably, in the ciltivating process of described arabidopsiss, the thickness of described second mixture layer is described liquid training
The 1/6～1/3 of nutrient solution depth.
Preferably, in the ciltivating process of described arabidopsiss, Vermiculitum and described shellfish described in described first mixture layer
The mass ratio of shell Calcium Carbonate is 3～5:5～3.
Preferably, in the ciltivating process of described arabidopsiss, Vermiculitum and described shellfish described in described second mixture layer
The mass ratio of eggshell powder is 1:1.
The present invention at least includes following beneficial effect：
The present invention soaks to seed initially with alcoholic solution, carries out subsequent step afterwards, can effectively remove kind
There is insect soft and antibacterial etc. in son, remove antibacterial that may contain in seed etc., sprout in solid medium simultaneously, enter one
Step decreases contaminated possibility in liquid medium；
The first mixture layer in the liquid medium of the present invention also has Vermiculitum and shell Calcium Carbonate, and one is to absorb foot
Enough nutritional solutions, keep the liquid humidity in culture and nutritional labeling, two is that shell Calcium Carbonate can effectively suppress growing of antibacterial,
Keep water body clean；The eggshell powder containing in second mixture layer equally also have suppression bacteria breed effect, simultaneously this
Several compositions can promote the growth of plant, achieves many things at one stroke；
Using the period of liquid medium Cyclic culture to plant strain growth to demand, the Material growth getting is consistent, raw
Long status are good, and no living contaminantses, are that good preparation is carried out in the accurate research of scientific experimentss.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
It should be appreciated that used herein such as " have ", "comprising" and " inclusion " term do not allot one or many
The presence of individual other element or a combination thereof or interpolation.
The present invention provides a kind of ciltivating process of arabidopsiss, comprises the steps：
Step one, arabidopsiss seed is put in the alcoholic solution of mass-volume concentration 60%-90% and soaks 10-30min,
Process 3～4 days at 0～4 DEG C of temperature and dark condition, then be placed in solid medium and cultivate into long more than the 1.2cm of root
Seedling；
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and at this
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of liquid medium, in 18～22 DEG C of temperature, humidity
60%～80%, under conditions of illumination 2000～3000lux and 24h illumination, cultivate 10～15 days, obtain seedling；
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell of this new liquid medium
Second mixture layer of powder, in 18～22 DEG C of temperature, humidity 60%～80%, illumination 2000～3000lux and 24h illumination
Under the conditions of, continue culture seedling 10～15 days；
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
The present invention soaks to seed initially with alcoholic solution, carries out subsequent step afterwards, can effectively remove kind
There is insect soft and antibacterial etc. in son, remove antibacterial that may contain in seed etc., sprout in solid medium simultaneously, enter one
Step decreases contaminated possibility in liquid medium；
The first mixture layer in the liquid medium of the present invention also has Vermiculitum and shell Calcium Carbonate, and one is to absorb foot
Enough nutritional solutions, keep the liquid humidity in culture and nutritional labeling, two is that shell Calcium Carbonate can effectively suppress growing of antibacterial,
Keep water body clean；The eggshell powder containing in second mixture layer equally also have suppression bacteria breed effect, simultaneously this
Several compositions can promote the growth of plant, achieves many things at one stroke；
Using the period of liquid medium Cyclic culture to plant strain growth to demand, the Material growth getting is consistent, raw
Long status are good, and no living contaminantses, are that good preparation is carried out in the accurate research of scientific experimentss.
In one of embodiment of the present invention, preferably, in the ciltivating process of described arabidopsiss, described solid
Culture medium is 1/2MS solid medium.
In one of embodiment of the present invention, preferably, described liquid medium comprises：1/5～1/3MS,
0.1-1.0mg/L 6-BA, 0.05-0.5mg/L KT and 0.1-0.5mg/L IAA.
In one of embodiment of the present invention, preferably, the thickness of described first mixture layer is described liquid
The 1/5～1/3 of medium depth.
In one of embodiment of the present invention, preferably, the thickness of described second mixture layer is described liquid
The 1/6～1/3 of medium depth.
In one of embodiment of the present invention, preferably, Vermiculitum described in described first mixture layer and described
The mass ratio of shell Calcium Carbonate is 3～5:5～3.
In one of embodiment of the present invention, preferably, Vermiculitum described in described second mixture layer and described
The mass ratio of shellfish eggshell powder is 1:1.
Embodiment 1
A kind of ciltivating process of arabidopsiss, comprises the steps：
Step one, arabidopsiss seed is put into and in the alcoholic solution of mass-volume concentration 60%, soaks 10min, after temperature
Process 3 days under 0 DEG C of degree and dark condition, then be placed in the seedling cultivating into long more than the 1.2cm of root in solid medium；Described solid
Culture medium is 1/2MS solid medium.
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and at this
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of liquid medium, in 18 DEG C of temperature, humidity 60%, light
Under conditions of 2000～3000lux and 24h illumination, cultivate 10 days, obtain seedling；The thickness of described first mixture layer is institute
State the 1/5 of liquid medium depth.The mass ratio of Vermiculitum and described shell Calcium Carbonate described in described first mixture layer is 3:
5.
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell of this new liquid medium
Second mixture layer of powder, under conditions of 18 DEG C of temperature, humidity 60%%, illumination 2000～ux and 24h illumination, continues training
Foster seedling 10 days；The thickness of described second mixture layer is the 1/6 of described liquid medium depth.In described second mixture layer
The mass ratio of described Vermiculitum and described shellfish eggshell powder is 1:1.
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
Described liquid medium comprises：1/5MS, 0.1mg/L 6-BA, 0.05mg/L KT and 0.1mg/L IAA.
Embodiment 2
A kind of ciltivating process of arabidopsiss, comprises the steps：
Step one, arabidopsiss seed is put into and in the alcoholic solution of mass-volume concentration 90%, soaks 30min, after temperature
Process 4 days under 4 DEG C of degree and dark condition, then be placed in the seedling cultivating into long more than the 1.2cm of root in solid medium；Described solid
Culture medium is 1/2MS solid medium.
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and at this
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of liquid medium, in 22 DEG C of temperature, humidity 80%, light
Under conditions of 3000lux and 24h illumination, cultivate 15 days, obtain seedling；The thickness of described first mixture layer is described liquid
The 1/3 of medium depth.The mass ratio of Vermiculitum and described shell Calcium Carbonate described in described first mixture layer is 5:3.
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell of this new liquid medium
Second mixture layer of powder, under conditions of 22 DEG C of temperature, humidity 80%, illumination 3000lux and 24h illumination, continues culture
Seedling 15 days；The thickness of described second mixture layer is the 1/3 of described liquid medium depth.Institute in described second mixture layer
Stating Vermiculitum and the mass ratio of described shellfish eggshell powder is 1:1.
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
Described liquid medium comprises：1/3MS, 1.0mg/L 6-BA, 0.5mg/L KT and 0.5mg/L IAA.
Embodiment 3
A kind of ciltivating process of arabidopsiss, comprises the steps：
Step one, arabidopsiss seed is put into and in the alcoholic solution of mass-volume concentration 75%, soaks 20min, after temperature
Process 3 days under 24 DEG C of degree and dark condition, then be placed in the seedling cultivating into long more than the 1.2cm of root in solid medium；Described solid
Body culture medium is 1/2MS solid medium.
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and at this
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of liquid medium, in 20 DEG C of temperature, humidity 70%, light
Under conditions of 2500lux and 24h illumination, cultivate 12.5 days, obtain seedling；The thickness of described first mixture layer is described liquid
The 1/4 of body medium depth.The mass ratio of Vermiculitum and described shell Calcium Carbonate described in described first mixture layer is 5:5.
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell of this new liquid medium
Second mixture layer of powder, under conditions of 20 DEG C of temperature, humidity 70%, illumination 2500lux and 24h illumination, continues culture
Seedling 12.5 days；The thickness of described second mixture layer is the 1/4 of described liquid medium depth.In described second mixture layer
The mass ratio of described Vermiculitum and described shellfish eggshell powder is 1:1.
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
Described liquid medium comprises：1/4MS, 0.55mg/L 6-BA, 0.3mg/L KT and 0.3mg/L IAA.
Module number described herein and treatment scale are used to simplify the explanation of the present invention.Water planting side to the present invention
The application of method, modifications and variations will be readily apparent to persons skilled in the art.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the embodiment with description.
Claims (7)
1. a kind of ciltivating process of arabidopsiss is it is characterised in that comprise the steps：
Step one, arabidopsiss seed is put into and soaks 10-30min in the alcoholic solution of mass-volume concentration 60%-90%, afterwards
Process 3～4 days at 0～4 DEG C of temperature and dark condition, then be placed in the children cultivating into long more than the 1.2cm of root in solid medium
Seedling；
Step 2, the seedling of long for the root obtaining in step one more than 1.2cm is moved in liquid medium culture, and in this liquid
The first mixture layer of one layer of Vermiculitum and shell Calcium Carbonate laid by the bottom of culture fluid, in 18～22 DEG C of temperature, humidity 60%～
80%th, under conditions of illumination 2000～3000lux and 24h illumination, cultivate 10～15 days, obtain seedling；
Step 3, liquid medium of replacing, simultaneously in low layer laying Vermiculitum and the eggshell powder of this new liquid medium
The second mixture layer, in the condition of 18～22 DEG C of temperature, humidity 60%～80%, illumination 2000～3000lux and 24h illumination
Under, continue culture seedling 10～15 days；
Step 4, circulating repetition step 2 and step 3, until arabidopsiss seedling is cultivated to the period of demand.
2. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that described solid medium is 1/2MS solid
Culture medium.
3. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that described liquid medium comprises：1/5～1/
3MS, 0.1-1.0mg/L 6-BA, 0.05-0.5mg/L KT and 0.1-0.5mg/L IAA (strong sprout).
4. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that the thickness of described first mixture layer is institute
State the 1/5～1/3 of liquid medium depth.
5. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that the thickness of described second mixture layer is institute
State the 1/6～1/3 of liquid medium depth.
6. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that Vermiculitum described in described first mixture layer
Mass ratio with described shell Calcium Carbonate is 3～5:5～3.
7. the ciltivating process of arabidopsiss as claimed in claim 1 is it is characterised in that Vermiculitum described in described second mixture layer
Mass ratio with described shellfish eggshell powder is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610787994.5A CN106465675A (en) | 2016-08-31 | 2016-08-31 | A kind of ciltivating process of arabidopsiss |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610787994.5A CN106465675A (en) | 2016-08-31 | 2016-08-31 | A kind of ciltivating process of arabidopsiss |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106465675A true CN106465675A (en) | 2017-03-01 |
Family
ID=58230020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610787994.5A Pending CN106465675A (en) | 2016-08-31 | 2016-08-31 | A kind of ciltivating process of arabidopsiss |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106465675A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419657A (en) * | 2018-02-08 | 2018-08-21 | 贵州省油菜研究所 | A kind of technical method of arabidopsis high effect culture |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1902997A (en) * | 2006-08-02 | 2007-01-31 | 中国科学院植物研究所 | Method for cultivation of arabidopsis |
| CN101142893A (en) * | 2007-10-09 | 2008-03-19 | 浙江大学 | Water-planting method for arabidopsis thaliana |
| CN102265780A (en) * | 2011-05-15 | 2011-12-07 | 浙江大学 | Simple seedling raising method of Arabidopsis seeds and used device |
| CN102696463A (en) * | 2012-06-07 | 2012-10-03 | 山东理工大学 | Arabidopsis hydroponic method capable of effectively preventing generation of green algae |
| CN104273119A (en) * | 2014-09-15 | 2015-01-14 | 上海交通大学 | Method for improving storage effect of arabidopsis thaliana seedlings |
| CN204762639U (en) * | 2015-07-02 | 2015-11-18 | 中国农业科学院茶叶研究所 | Arabidopsis water planting device |
-
2016
- 2016-08-31 CN CN201610787994.5A patent/CN106465675A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1902997A (en) * | 2006-08-02 | 2007-01-31 | 中国科学院植物研究所 | Method for cultivation of arabidopsis |
| CN101142893A (en) * | 2007-10-09 | 2008-03-19 | 浙江大学 | Water-planting method for arabidopsis thaliana |
| CN102265780A (en) * | 2011-05-15 | 2011-12-07 | 浙江大学 | Simple seedling raising method of Arabidopsis seeds and used device |
| CN102696463A (en) * | 2012-06-07 | 2012-10-03 | 山东理工大学 | Arabidopsis hydroponic method capable of effectively preventing generation of green algae |
| CN104273119A (en) * | 2014-09-15 | 2015-01-14 | 上海交通大学 | Method for improving storage effect of arabidopsis thaliana seedlings |
| CN204762639U (en) * | 2015-07-02 | 2015-11-18 | 中国农业科学院茶叶研究所 | Arabidopsis water planting device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419657A (en) * | 2018-02-08 | 2018-08-21 | 贵州省油菜研究所 | A kind of technical method of arabidopsis high effect culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN101946709B (en) | Seedling raising method of plateau rhodiola crenulata | |
| WO2018218914A1 (en) | Seedling substrate for solanaceous vegetables and producing method therefor | |
| CN105993865A (en) | Cultivation method for quercus variabilis aseptic seedling | |
| CN104067939A (en) | Tissue culture rapid propagation method of radix gentianae | |
| CN104871964A (en) | Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue | |
| CN102217551B (en) | Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips | |
| CN103125393B (en) | Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am | |
| CN106069772A (en) | A kind of sword-leaved cymbidium tissue culture quick propagation culturing method | |
| CN108371003A (en) | A kind of breeding method of bletilla striata seeds | |
| CN106561110A (en) | Germination accelerating method of peony seeds | |
| CN106465675A (en) | A kind of ciltivating process of arabidopsiss | |
| CN108157098A (en) | A kind of acclimatization and transplants method of pale reddish brown trident Herba Phaii tankervilliaes tissue culture rooted seedling | |
| CN104429968A (en) | Tissue culture method of premna fulva | |
| JP7454587B2 (en) | Plant growth regulators and their applications | |
| CN1600082A (en) | Tissue culture technique for peony | |
| CN106386431A (en) | Bacteriostatic hydroponics method of Arabidopsis | |
| CN106376446A (en) | Method for prolonging water planting time of arabidopsis thaliana | |
| CN105638298A (en) | Culture medium capable of increasing transplanting survival rate of saffron crocus and preparation method thereof | |
| CN106386433A (en) | Method for cultivation of Arabidopsis | |
| CN106718867A (en) | A kind of method that tomato tissue culture is carried out in test tube | |
| CN102640656B (en) | Efficient seedling exercising transplanting method for raspberry tissue culture seedlings | |
| CN106613970B (en) | The quick breeding by group culture method of sealwort leaf elegant jessamine | |
| CN103444537A (en) | Tissue culture and rapid propagation method of rhizoma arisaematis | |
| CN104206071A (en) | Rapid propagation method of alder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180615 Address after: 362200 No. 1306, Antai world city, Luoshan street, Jinjiang, Quanzhou, Fujian Applicant after: Quanzhou Qimei Electronic Technology Co. Ltd. Address before: 536000 15 Lane 2 lane, vegetable garden, Haicheng District, Beihai, the Guangxi Zhuang Autonomous Region Applicant before: Huang Fuping |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170301 |
|
| RJ01 | Rejection of invention patent application after publication |