CN105830888B - A kind of method of stinkgrass flower live streaming water planting - Google Patents
A kind of method of stinkgrass flower live streaming water planting Download PDFInfo
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- CN105830888B CN105830888B CN201610195319.3A CN201610195319A CN105830888B CN 105830888 B CN105830888 B CN 105830888B CN 201610195319 A CN201610195319 A CN 201610195319A CN 105830888 B CN105830888 B CN 105830888B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
A kind of method of stinkgrass flower live streaming water planting, it is related to a kind of method of stinkgrass flower live streaming water planting.It is irregular in the presence of germinateing that the present invention is to solve existing stinkgrass flower cultural methods, and inconvenience is not enough applied in flexibility, cumbersome easily by green alga pollution, the problem of can not implementing on a large scale.Method：One, water planting field planting basket is processed；Two, the assembling of cultivating container；Three, Puccinellia Tenuiflora Seeds processing and sowing；Four, the culture of Puccinellia Tenuiflora.The present invention for being broadcast live water planting stinkgrass flower on a large scale.
Description
Technical field
The present invention relates to a kind of methods that water planting is broadcast live in stinkgrass flower.
Background technology
Stinkgrass flower (Puccinellia tenuiflora) is a kind of grass family alkali lance category Saline Forage, is distributed mainly on
The ground such as China northeast, North China, northwest, the Inner Mongol and Qinghai.Stinkgrass flower has very strong drought-enduring, cold-resistant, salt tolerant alkali ability, can be
The saline and alkaline aerial that pH is 9~10.Therefore, stinkgrass flower can as research saline-alkali tolerant mechanism a kind of important model plant,
Important evidence is provided to cultivate the crops such as resistant wheats, the rice such as tool saline-alkali tolerant.Meanwhile stinkgrass flower blade is soft delicate, fits
Mouth property is good, can be as the excellent forage grass of livestock.Therefore, to the degeneration-resistant mechanism of stinkgrass flower research have important theory significance and
Application value.In recent years, people are constantly ground using ecology, physiology, molecular biology, transcription group and proteomics etc.
Study carefully strategy, the saline-alkali tolerant mechanism of stinkgrass flower is studied.With going deep into for research, to a large amount of, quickly breeding stinkgrass flower children
More stringent requirements are proposed for seedling.
For a long time, the breeding method of plant seedlings mainly has two kinds of earth culture method and water culture.Earth culture method is because easy to operate
And be widely adopted, but due to cannot strictly control the nutritional ingredient in solid dielectric, earth culture method gives some research work bands
Inconvenience is carried out, while being also unfavorable for the observation of root growth state and taking a sample to research.The nutrient solution generation of water culture equilibrium
The soil or matrix that traditional plant is cultivated are replaced.Water planting can provide stably and controllable culture systems, can be wanted according to experiment
The ingredient for accurately controlling culture solution is sought, and convenient for observation and materials.Water culture becomes the powerful measure of plant culture.
Water culture method mostly uses method for transplanting at present.Seed is first generally seeded in MS culture mediums or other earth culture bases
Matter, then gained seedling will be cultivated and be transferred to hydroponic system.But since stinkgrass flower belongs to fibrous root herbaceous plant, root system is fragile, is moving
Seedling is easily hurt during cultivation, and survival rate is low.Therefore, method for transplanting is not appropriate for the water planting culture of stinkgrass flower.
Water planting direct sowing method, which mostly uses seed point being sowed on stainless steel mesh or cotton gauze, carries out water planting.It include sowing
It is all carried out in the apparatus with the entire plant strain growth process including germination, without transplanting.But very due to Puccinellia Tenuiflora Seeds
Tiny, the seed on sieve is difficult to fixation and easily floats, and growth interface is unstable to be caused to germinate irregular, and flexibility is not enough answered
With inconvenience, and it is protected from light and easily by green alga pollution since sieve cannot achieve.When the groups such as extraction root system of plant RNA, DNA, protein
Timesharing, Chang Yinwei root systems are polluted by green alga, to influence experimental result, or even can because experiment material does not reach requirement can not
Carry out experiment.
Currently, researcher using culture medium with the hydroponic system that nutrient solution is combined carry out arabidopsis etc. should not be into
The water planting culture of row transplanting plant.For example, utilizing Eppendorf centrifuge tubes and centrifuge tube box or pipette tips after artificial
Box suit, arabidopsis seed are not necessarily to that the combination sprouted on agar and grown in mill water culture nutrient solution can be realized by transplanting.
But these experimental provision post-production need to be carried out manually, and it is mostly cumbersome, it takes time and effort, can not implement on a large scale.Also, it cultivates
Moisture is very easy to evaporation in base, it is necessary to and nutrient solution is replenished in time, solid medium is kept to be contacted with liquid level, once without timely
Additional nutrient solution causes culture medium dehydration withered, and seedling is likely to water shortage and dead.Currently, culture, Prototheca can be broadcast live on a large scale
Pollution, well developed root system, simple and practicable and flexible herbaceous plant hydroponic device and ciltivating process are actually rare.
Invention content
It is irregular in the presence of germinateing that the present invention is to solve existing stinkgrass flower cultural methods, and inconvenience is not enough applied in flexibility, easily
It is cumbersome by green alga pollution, the problem of can not implementing on a large scale, and provide a kind of method of stinkgrass flower live streaming water planting.
A kind of method of stinkgrass flower live streaming water planting specifically carries out according to the following steps：
One, water planting field planting basket is processed：Nylon screen is fixed on to bottom surface and the inner wall of water planting field planting basket, the nylon screen
Outer envelope the edge of water planting field planting basket, then the outer wall of water planting field planting basket is wound up by waterproof friction tape, together
When the outer of nylon screen and the edge of water planting field planting basket are fixed together by waterproof friction tape, using glass bar from water planting
Nylon screen is ejected the basket open of water planting field planting basket by the eyelet of field planting basket bottom, then by nylon screen and water planting field planting basket bottom surface
It in the part complete wetting of contact to heated dissolved a concentration of 1% agar solution, takes out, then uses glass bar will
Nylon screen retightens the water planting field planting basket that agar has been infiltrated in the bottom surface of water planting field planting basket and inner wall, stand 5~
The vermiculite that grain size is 1mm~3mm is added to agar solidification, then into the water planting field planting basket for infiltrated agar by 10min, and grain is added
The thickness for the vermiculite that diameter is 1mm~3mm is 1cm, makes vermiculite surfacing using shaking, the water planting field planting basket after being processed；
Two, the assembling of cultivating container：Water planting field planting basket after processing is placed in iris pallet, the iris pallet
It is placed in culture side's basin, agar and vermiculite of the nutrient solution in the water planting field planting basket to after processing then is added into culture side's basin all
It is immersed in nutrient solution, position extra in culture side's basin after placement iris pallet is covered using black K T cystosepiments, is obtained
To hydroponic device；
Three, Puccinellia Tenuiflora Seeds processing and sowing：By stinkgrass flower dry seeds dark and be placed in distilled water impregnate at room temperature
For 24 hours, then the seed after being impregnated is filtered dry the seed after immersion using four layers of cotton gauze, the kind after being filtered dry
Son, by the seed after being filtered dry and vermiculite by volume 1:2 mixings are sprinkled into the water planting field planting basket in the hydroponic device that step 2 obtains
It is interior, it is then then covered with the vermiculite that a layer thickness is 2~3mm, then uses watering can to soak thickness for the vermiculite of 2~3mm, then adopt
Hydroponic device is sealed at room temperature with preservative film, and opaque-plastic cloth is thickeied using black outside preservative film and is blocked, is obtained
Plant the hydroponic device for needing to be cultivated Puccinellia Tenuiflora；
Four, the culture of Puccinellia Tenuiflora：The hydroponic device that the cultivation that step 3 obtains is needed to be cultivated Puccinellia Tenuiflora shifts
To between culture, seed is sprouted after 3~5 days, is opened after black thickeies opaque-plastic cloth and preservative film and is replaced nutrient solution, later
One time of nutrition liquid was replaced every five days, until stinkgrass flower is ripe, that is, stinkgrass flower is completed and water planting is broadcast live.
The beneficial effects of the invention are as follows：
1, the present invention is by processing water planting field planting basket, in conjunction with the advantages of earth culture and water planting, realizes the extensive straight of stinkgrass flower
Broadcast culture.Nylon screen in hydroponic device can be as the supporter of agar, and agar can both keep the penetrating of nutrient solution
Property, and can prevent vermiculite placed on it from being leaked out from nylon screen gap, avoid having vermiculite on grown root system, shadow
Ring the acquisition of material.And the vermiculite covered on agar can be protected due to its strong absorptive by absorbing nutrient solution in culture side's basin
Moisture state is held, to which seed can be made to sprout and grow and keep the moistening of bottom agar, prevents the easy desiccation of agar
And it is ineffective.And seed can be stably fixed on vermiculite, and growth interface is steady, and germination is neat.Therefore, the present invention is big
It is big that seed point is directly sowed to the water planting direct sowing method carried out on sieve or cotton gauze better than before, it is used also superior to simple before
The water planting direct sowing method that culture medium is combined with nutrient solution.
2, water planting field planting basket used in the present invention, iris pallet and culture side's basin match simple each other
It is easy.The height of iris pallet is higher than water planting field planting basket, can hold up water planting field planting basket, and complementary space is stayed for the growth of root.Training
Foster side's basin is more slightly larger than iris pallet, the position the having more black K T cystosepiments covering that can be raised, convenient for being added in Culture basin
Nutrient solution is also convenient for the liquid level situation of change of observation nutrient solution and the growth conditions of root.
3, the present invention is not necessarily to sterile working.The present invention uses black mackintosh looping, application around water planting field planting basket
Black iris pallet surrounds culture side's basin using black polybag is thickeied, and covers iris support using black K T cystosepiments
Extra gap outside disk can minimize influence of the illumination to root growth, while prevented also from the breeding of green alga.
4, hydroponic device using flexible of the invention.The related water planting field planting basket of iris pallet can be taken out, facilitate nutrition
The replacement of liquid.Importantly, because each water planting field planting basket is movable, wait for that stinkgrass flower is big needed for growing to
After small, can according to required stinkgrass flower dosage number, select culture side basin of corresponding size and iris pallet, it is desirable wherein
Multiple or single water planting field planting baskets carry out different Stress treatments.
5, the cultivating containers such as water planting field planting basket used in the present invention, iris pallet and culture side's basin are cheap, after
Phase processing is simple.Moreover, these cultivating containers are all unlikely to deform, and it is reusable, to reduce cost, be conducive to train on a large scale
It supports.
Description of the drawings
Fig. 1 is the Puccinellia Tenuiflora photo after the present embodiment is cultivated 50 days.
Specific implementation mode
Specific implementation mode one：A kind of method of present embodiment stinkgrass flower live streaming water planting be specifically according to the following steps into
Row：
One, water planting field planting basket is processed：Nylon screen is fixed on to bottom surface and the inner wall of water planting field planting basket, the nylon screen
Outer envelope the edge of water planting field planting basket, then the outer wall of water planting field planting basket is wound up by waterproof friction tape, together
When the outer of nylon screen and the edge of water planting field planting basket are fixed together by waterproof friction tape, using glass bar from water planting
Nylon screen is ejected the basket open of water planting field planting basket by the eyelet of field planting basket bottom, then by nylon screen and water planting field planting basket bottom surface
It in the part complete wetting of contact to heated dissolved a concentration of 1% agar solution, takes out, then uses glass bar will
Nylon screen retightens the water planting field planting basket that agar has been infiltrated in the bottom surface of water planting field planting basket and inner wall, stand 5~
The vermiculite that grain size is 1mm~3mm is added to agar solidification, then into the water planting field planting basket for infiltrated agar by 10min, and grain is added
The thickness for the vermiculite that diameter is 1mm~3mm is 1cm, makes vermiculite surfacing using shaking, the water planting field planting basket after being processed；
Two, the assembling of cultivating container：Water planting field planting basket after processing is placed in iris pallet, the iris pallet
It is placed in culture side's basin, agar and vermiculite of the nutrient solution in the water planting field planting basket to after processing then is added into culture side's basin all
It is immersed in nutrient solution, position extra in culture side's basin after placement iris pallet is covered using black K T cystosepiments, is obtained
To hydroponic device；
Three, Puccinellia Tenuiflora Seeds processing and sowing：By stinkgrass flower dry seeds dark and be placed in distilled water impregnate at room temperature
For 24 hours, then the seed after being impregnated is filtered dry the seed after immersion using four layers of cotton gauze, the kind after being filtered dry
Son, by the seed after being filtered dry and vermiculite by volume 1:2 mixings are sprinkled into the water planting field planting basket in the hydroponic device that step 2 obtains
It is interior, it is then then covered with the vermiculite that a layer thickness is 2~3mm, then uses watering can to soak thickness for the vermiculite of 2~3mm, then adopt
Hydroponic device is sealed at room temperature with preservative film, and opaque-plastic cloth is thickeied using black outside preservative film and is blocked, is obtained
Plant the hydroponic device for needing to be cultivated Puccinellia Tenuiflora；
Four, the culture of Puccinellia Tenuiflora：The hydroponic device that the cultivation that step 3 obtains is needed to be cultivated Puccinellia Tenuiflora shifts
To between culture, seed is sprouted after 3~5 days, is opened after black thickeies opaque-plastic cloth and preservative film and is replaced nutrient solution, later
One time of nutrition liquid was replaced every five days, until stinkgrass flower is ripe, that is, stinkgrass flower is completed and water planting is broadcast live.
Nylon screen in present embodiment hydroponic device can be as the supporter of agar, and agar can both keep seeking
The permeability of nutrient solution, and can prevent vermiculite placed on it from being leaked out from nylon screen gap, it avoids on grown root system
With vermiculite, the acquisition of material is influenced.And the vermiculite covered on agar can pass through absorption culture side's basin due to its strong absorptive
Middle nutrient solution keeps moisture state, to which seed can be made to sprout and grow and keep the moistening of bottom agar, prevents agar
Easy desiccation and it is ineffective.And seed can be stably fixed on vermiculite, and growth interface is steady, and germination is neat.
Present embodiment is selected above the iris pallet of water planting field planting basket so that iris pallet can be by water planting field planting basket
It holds up, complementary space is stayed for the growth of root.Culture side's basin of selection is more slightly larger than iris pallet used.Black K T cystosepiments can lift
It opens, facilitates and nutrient solution is added into Culture basin, be also convenient for the growth conditions of the liquid level and root of observation nutrient solution, also can avoid
The evaporation of moisture in nutrient solution.The waterproof friction tape of winding water planting field planting basket used in this hydroponic device, the black of application
Iris pallet covers the black K T cystosepiments in extra gap outside iris pallet, and surrounds the black thickening of culture side's basin
Opaque-plastic cloth or masking foil can minimize influence of the illumination to root growth, while prevent the breeding of green alga.
Specific implementation mode two：The present embodiment is different from the first embodiment in that：Nylon mesh described in step 1
The mesh number of net is 20 mesh.Other are same as the specific embodiment one.
Specific implementation mode three：The present embodiment is different from the first and the second embodiment in that：Water described in step 1
The size for training field planting basket is overall diameter 8cm, interior diameter 6cm, high 3.4cm.Other are the same as one or two specific embodiments.
Specific implementation mode four：Unlike one of present embodiment and specific implementation mode one to three：Institute in step 2
The size for stating iris pallet is long 45cm, width 27cm, high 7cm, hole outer diameter 7cm.Other and specific implementation mode one to three it
One is identical.
Specific implementation mode five：Unlike one of present embodiment and specific implementation mode one to four：Institute in step 2
The size for stating culture side's basin is long 52cm, width 30cm, high 13.5cm.Other are identical as one of specific implementation mode one to four.
Specific implementation mode six：Unlike one of present embodiment and specific implementation mode one to five：Institute in step 2
State Ca (NO in nutrient solution3)2·4H2The content of O is 1180mg/L, KNO3Content be 506mg/L, KH2PO4Content be
136mg/L、MgSO4·7H2The content of O is 493mg/L, FeSO4·7H2The content of O is 27.8mg/L, EDTA2Na2Content
For 37.2mg/L, H3BO3Content be 28.6mg/L, MnSO4·H2The content of O is 16.9mg/L, ZnSO4·7H2The content of O is
2.1mg/L、CuSO4·5H2The content of O is 0.8mg/L, (NH4)6MoO4·4H2The content of O is 0.2mg/L；The nutrient solution
PH is 6.0.Other are identical as one of specific implementation mode one to five.
The present invention further improves the object of trace element on the basis of the Hoagland nutrient solutions of widely applied improvement
Matter forms and content, and the NH contained in the Hoagland nutrient solutions of improvement is not added4NO3, KI, CoCl2And Na2MoO4。
Because stinkgrass flower is concentrated mainly on its research and responses to which the salt stress such as NaCl as a kind of important salt tolerant pattern species
Research, therefore present embodiment excludes Cl ions and Na ions in nutrient solution, and the stinkgrass flower that it is cultivated is made to be suitable for NaCl
The research of the environment stresses such as stress.And because present embodiment is strictly protected from light, therefore it is micro- in nutrient solution to avoid KI need not to be added
The breeding of biology.Meanwhile in order to which the missing for making up these substances leads to subalimentation, (NH is added in nutrient solution4)6MoO4·4H2O, while increasing Ca (NO3)2·4H2The content of O.
Specific implementation mode seven：Unlike one of present embodiment and specific implementation mode one to six：Step 3 culminant star
The volume ratio of water planting field planting basket after the processing that the quality of star grass dry seeds is obtained with step 1 is 0.5g:(80~100) cm3。
Other are identical as one of specific implementation mode one to six.
Specific implementation mode eight：Unlike one of present embodiment and specific implementation mode one to seven：Institute in step 4
State culture between parameter be：Day night temperature is 25 DEG C/20 DEG C, and periodicity of illumination is 12h illumination/12h dark, intensity of illumination 300
μmol·m-2·s-1, relative humidity 75%.Other are identical as one of specific implementation mode one to seven.
Specific implementation mode nine：Unlike one of present embodiment and specific implementation mode one to eight：Institute in step 4
State Ca (NO in nutrient solution3)2·4H2The content of O is 1180mg/L, KNO3Content be 506mg/L, KH2PO4Content be
136mg/L、MgSO4·7H2The content of O is 493mg/L, FeSO4·7H2The content of O is 27.8mg/L, EDTA2Na2Content
For 37.2mg/L, H3BO3Content be 28.6mg/L, MnSO4·H2The content of O is 16.9mg/L, ZnSO4·7H2The content of O is
2.1mg/L、CuSO4·5H2The content of O is 0.8mg/L, (NH4)6MoO4·4H2The content of O is 0.2mg/L；The nutrient solution
PH is 6.0.Other are identical as one of specific implementation mode one to eight.
Advantageous effect of the present invention is verified using following embodiment：
Embodiment one：A kind of method of stinkgrass flower live streaming water planting specifically carries out according to the following steps：
One, water planting field planting basket is processed：Nylon screen is fixed on to bottom surface and the inner wall of water planting field planting basket, the nylon screen
Outer envelope the edge of water planting field planting basket, then the outer wall of water planting field planting basket is wound up by waterproof friction tape, together
When the outer of nylon screen and the edge of water planting field planting basket are fixed together by waterproof friction tape, using glass bar from water planting
Nylon screen is ejected the basket open of water planting field planting basket by the eyelet of field planting basket bottom, then by nylon screen and water planting field planting basket bottom surface
It in the part complete wetting of contact to heated dissolved a concentration of 1% agar solution, takes out, then uses glass bar will
Nylon screen retightens the water planting field planting basket that agar has been infiltrated in the bottom surface of water planting field planting basket and inner wall, stand 5~
The vermiculite that grain size is 1mm~3mm is added to agar solidification, then into the water planting field planting basket for infiltrated agar by 10min, and grain is added
The thickness for the vermiculite that diameter is 1mm~3mm is 1cm, makes vermiculite surfacing using shaking, the water planting field planting basket after being processed；
Two, the assembling of cultivating container：Water planting field planting basket after processing is placed in iris pallet, the iris pallet
It is placed in culture side's basin, agar and vermiculite of the nutrient solution in the water planting field planting basket to after processing then is added into culture side's basin all
It is immersed in nutrient solution, position extra in culture side's basin after placement iris pallet is covered using black K T cystosepiments, is obtained
To hydroponic device；
Three, Puccinellia Tenuiflora Seeds processing and sowing：0.5g stinkgrass flowers dry seeds in dark and are placed in distilled water at room temperature
It impregnates for 24 hours, then the seed after being impregnated is filtered dry the seed after immersion using four layers of cotton gauze, after being filtered dry
Seed, by the seed after being filtered dry and vermiculite by volume 1:It is fixed that 2 mixings are sprinkled into the water planting in the hydroponic device that step 2 obtains
It plants in basket, is then then covered with the vermiculite that a layer thickness is 2~3mm, watering can is then used to soak thickness for the vermiculite of 2~3mm,
Hydroponic device is sealed at room temperature using preservative film again, and opaque-plastic cloth is thickeied using black outside preservative film and is blocked,
It obtains planting the hydroponic device for needing to be cultivated Puccinellia Tenuiflora；
Four, the culture of Puccinellia Tenuiflora：The hydroponic device that the cultivation that step 3 obtains is needed to be cultivated Puccinellia Tenuiflora shifts
To between culture, seed is sprouted after 3~5 days, is opened after black thickeies opaque-plastic cloth and preservative film and is replaced nutrient solution, later
One time of nutrition liquid was replaced every five days, until stinkgrass flower is ripe, that is, stinkgrass flower is completed and water planting is broadcast live.
The mesh number of nylon screen described in step 1 is 20 mesh.
The size of water planting field planting basket described in step 1 is overall diameter 8cm, interior diameter 6cm, high 3.4cm.
The size of iris pallet described in step 2 is long 45cm, width 27cm, high 7cm, hole outer diameter 7cm.
The size of culture side's basin described in step 2 is long 52cm, width 30cm, high 13.5cm.
In step 2, Ca (NO in nutrient solution described in step 43)2·4H2The content of O is 1180mg/L, KNO3Content
For 506mg/L, KH2PO4Content be 136mg/L, MgSO4·7H2The content of O is 493mg/L, FeSO4·7H2The content of O is
27.8mg/L、EDTA·2Na2Content be 37.2mg/L, H3BO3Content be 28.6mg/L, MnSO4·H2The content of O is
16.9mg/L、ZnSO4·7H2The content of O is 2.1mg/L, CuSO4·5H2The content of O is 0.8mg/L, (NH4)6MoO4·4H2O
Content be 0.2mg/L；The pH of the nutrient solution is 6.0.
Described in step 4 cultivate between parameter be：Day night temperature is 25 DEG C/20 DEG C, and periodicity of illumination is 12h illumination/12h
Dark, intensity of illumination are 300 μm of olm-2·s-1, relative humidity 75%.
Fig. 1 is the Puccinellia Tenuiflora photo after the present embodiment is cultivated 50 days；As can be seen from the figure Puccinellia Tenuiflora is grown
It is in good condition, well developed root system and Prototheca pollution, it is seen that the present embodiment can be obtained complete and free of contamination stinkgrass flower root system and be used for
Research.
Claims (9)
1. a kind of method of stinkgrass flower live streaming water planting, it is characterised in that the method that water planting is broadcast live in stinkgrass flower is specifically according to the following steps
It carries out：
One, water planting field planting basket is processed：Nylon screen is fixed on to bottom surface and the inner wall of water planting field planting basket, the nylon screen it is outer
Along the edge for enveloping water planting field planting basket, then the outer wall of water planting field planting basket is wound up by waterproof friction tape, simultaneously will
The outer of nylon screen and the edge of water planting field planting basket are fixed together by waterproof friction tape, are colonized from water planting using glass bar
Nylon screen is ejected the basket open of water planting field planting basket by the eyelet of basket bottom, then contacts nylon screen with water planting field planting basket bottom surface
Part complete wetting to heated dissolved a concentration of 1% agar solution in, take out, then use glass bar by nylon
Sieve retightens the water planting field planting basket that agar has been infiltrated in the bottom surface of water planting field planting basket and inner wall, stands 5~10min
To agar solidification, the vermiculite that grain size is 1mm~3mm is then added into the water planting field planting basket for infiltrated agar, grain size, which is added, is
The thickness of the vermiculite of 1mm~3mm is 1cm, makes vermiculite surfacing using shaking, the water planting field planting basket after being processed；
Two, the assembling of cultivating container：Water planting field planting basket after processing is placed in iris pallet, the iris pallet is placed in
In culture side's basin, agar and vermiculite of the nutrient solution in the water planting field planting basket to after processing then are added into culture side's basin and all submerges
In nutrient solution, position extra in culture side's basin after placement iris pallet is covered using black K T cystosepiments, obtains water
Train device；
Three, Puccinellia Tenuiflora Seeds processing and sowing：By stinkgrass flower dry seeds dark and be placed in distilled water impregnate for 24 hours at room temperature,
Then seed after being impregnated is filtered dry the seed after immersion using four layers of cotton gauze, the seed after being filtered dry, will
Seed after being filtered dry and vermiculite by volume 1:2 mixings are sprinkled into the water planting field planting basket in the hydroponic device that step 2 obtains, so
It is then covered with the vermiculite that a layer thickness is 2~3mm afterwards, then uses watering can to soak thickness for the vermiculite of 2~3mm, then using guarantor
Fresh film seals hydroponic device at room temperature, and thickeies opaque-plastic cloth using black outside preservative film and block, and obtaining planting has
The hydroponic device of Puccinellia Tenuiflora to be cultivated；
Four, the culture of Puccinellia Tenuiflora：The hydroponic device that the cultivation that step 3 obtains is needed to be cultivated Puccinellia Tenuiflora is transferred to training
Between supporting, seed is sprouted after 3~5 days, is opened after black thickeies opaque-plastic cloth and preservative film and is replaced nutrient solution, later every
It replaces one time of nutrition liquid within five days, until stinkgrass flower is ripe, that is, completes stinkgrass flower and water planting is broadcast live.
2. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that nylon described in step 1
The mesh number of sieve is 20 mesh.
3. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that water planting described in step 1
The size of field planting basket is overall diameter 8cm, interior diameter 6cm, high 3.4cm.
4. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that butterfly described in step 2
The size of blue pallet is long 45cm, width 27cm, high 7cm, hole outer diameter 7cm.
5. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that cultivated described in step 2
The size of square basin is long 52cm, width 30cm, high 13.5cm.
6. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that nutrition described in step 2
Ca (NO in liquid3)2·4H2The content of O is 1180mg/L, KNO3Content be 506mg/L, KH2PO4Content be 136mg/L,
MgSO4·7H2The content of O is 493mg/L, FeSO4·7H2The content of O is 27.8mg/L, EDTANa2Content be 37.2mg/
L、H3BO3Content be 28.6mg/L, MnSO4·H2The content of O is 16.9mg/L, ZnSO4·7H2The content of O be 2.1mg/L,
CuSO4·5H2The content of O is 0.8mg/L, (NH4)6MoO4·4H2The content of O is 0.2mg/L；The pH of the nutrient solution is 6.0.
7. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that stinkgrass flower is dry in step 3
The volume ratio of water planting field planting basket after the processing that the quality of seed is obtained with step 1 is 0.5g:(80~100) cm3。
8. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that cultivated described in step 4
Between parameter be：Day night temperature is 25 DEG C/20 DEG C, and periodicity of illumination is 12h illumination/12h dark, and intensity of illumination is 300 μm of ol
m-2·s-1, relative humidity 75%.
9. a kind of method of stinkgrass flower live streaming water planting according to claim 1, it is characterised in that nutrition described in step 4
Ca (NO in liquid3)2·4H2The content of O is 1180mg/L, KNO3Content be 506mg/L, KH2PO4Content be 136mg/L,
MgSO4·7H2The content of O is 493mg/L, FeSO4·7H2The content of O is 27.8mg/L, EDTANa2Content be 37.2mg/
L、H3BO3Content be 28.6mg/L, MnSO4·H2The content of O is 16.9mg/L, ZnSO4·7H2The content of O be 2.1mg/L,
CuSO4·5H2The content of O is 0.8mg/L, (NH4)6MoO4·4H2The content of O is 0.2mg/L；The pH of the nutrient solution is 6.0.
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| CN101142893A (en) * | 2007-10-09 | 2008-03-19 | 浙江大学 | Water-planting method for arabidopsis thaliana |
| CN204653250U (en) * | 2015-04-30 | 2015-09-23 | 王润佳 | A kind of hydroponic device |
| CN105085067A (en) * | 2015-08-27 | 2015-11-25 | 江苏农林职业技术学院 | Special-purpose muskmelon nutritional liquid manure capable of improving saline soil, and preparation method and water planting method of special-purpose muskmelon nutritional liquid manure |
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| CN204653250U (en) * | 2015-04-30 | 2015-09-23 | 王润佳 | A kind of hydroponic device |
| CN105085067A (en) * | 2015-08-27 | 2015-11-25 | 江苏农林职业技术学院 | Special-purpose muskmelon nutritional liquid manure capable of improving saline soil, and preparation method and water planting method of special-purpose muskmelon nutritional liquid manure |
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| 一种适于营养胁迫研究的拟南芥水培方法;陈庆彬 等;《植物学报》;20141231;第49卷(第4期);第462-468页 * |
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