CN108738770B - Sea-buckthorn hardwood cutting seedling method - Google Patents
Sea-buckthorn hardwood cutting seedling method Download PDFInfo
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Abstract
The invention relates to a seabuckthorn hardwood cutting seedling method. A seabuckthorn hardwood cutting seedling method comprises the following steps: (1) selecting cutting; (2) shearing; (3) soaking the cutting shoots; (4) cutting the soaked cutting slips into a substrate; (5) watering every day after cuttage; (6) transplanting the seedlings 45-50 days after cuttage; picking buds 7-10 days after transplanting, and only leaving 1 bud tip; when the height of the nursery stock is 40-60cm, the nursery stock can be taken out of the nursery for forestation; (7) bud picking and micropropagation: selecting 5-7cm sprout tips from the removed sprout tips, repeating the steps (3) - (5), cutting for 40-50 days, transplanting, overwintering in a nursery land, and enabling the seedling height to reach more than 70cm in the next year, so that the seedlings can be planted. According to the method for raising seedlings of sea-buckthorn hardwood by cutting, the removed shoot is raised again in the process of raising seedlings of sea-buckthorn hardwood by cutting, so that the cutting coefficient is improved by 1-2 times, the survival rate is high, the survival rate of primary cutting is more than 90%, and the survival rate of secondary cutting can also reach more than 80%.
Description
Technical Field
The invention belongs to the technical field of seedling breeding, and particularly relates to a sea-buckthorn hardwood cutting seedling method.
Background
Seabuckthorn (Hippophae L.) is deciduous shrub or small tree of Hippophae genus of elaeagnus pungens, is a precious economic forest tree species containing most natural vitamins in the world at present, and the content of vitamin C is far higher than that of fresh jujube and kiwi fruit, so that the seabuckthorn is known as a treasure house of natural vitamins. The sea-buckthorn has high nutritional value, ecological value and economic value, especially plays an important role in the construction of the 'three-north' protection forest, and has become an economic tree species integrating ecological, economic and social benefits and is generally regarded by all countries in the world. In order to bring the sea-buckthorn into play with greater benefits, the cultivation of the sea-buckthorn is gradually changed from complete ecotype to ecological economy, and the improved variety and the intensive operation of sea-buckthorn cultivation become necessary trends of future sea-buckthorn resource development.
One remarkable characteristic of sea-buckthorn is that it is a male and female plant, the seedling progeny is highly heterozygous, the hereditary character variation is obvious, and more than 50% of the progeny are male plants, so it is necessary to obtain excellent plants with stable hereditary characters through asexual propagation, and especially, the breeding of rare fine varieties also requires asexual propagation. The sea-buckthorn seedlings are bred in a plurality of ways, such as grafting, root burying, layering, cuttage and the like, but the breeding technologies such as grafting, root burying, layering and the like are rarely adopted in large-scale sea-buckthorn seedling production, and the most adopted and most widely applied are cuttage breeding. Russian popularizes and applies hardwood cutting and twig cutting technologies, while Mongolia mainly uses twig cutting, such as full-illumination double-cantilever spray twig cutting and the like. A large number of clone seedlings with the genetic character consistent with that of the parent plant individuals can be quickly obtained through cutting propagation, the number of female plants is enlarged, and high yield is easy to realize after planting, so that the application of the cutting propagation is the widest.
The cutting propagation of sea-buckthorn generally adopts hardwood cutting and twig cutting. The twig cuttage is easy to take root, the propagation coefficient is high, and the method is widely applied to production. However, as the tender branch cuttage has more factors influencing rooting, the management is more complicated and the requirement is strict in the breeding process, and the nursery stock needs to be transplanted for 1.5-2.0 years to be outplanted, so that the time is long. But the survival rate and the propagation coefficient of the hardwood cutting are low, the use amount in the production is less, and the method is only applied to a few varieties which are easy to root.
In recent years, a seedling raising tray is utilized, and a water-retaining soil culture method is adopted to develop the research of sea-buckthorn hardwood cutting seedling raising test. Research results show that the method is simple and easy to implement, high in operability and high in survival rate, and can be used for cutting in the same year and outplanting in the same year. However, in the process of cutting seedling raising by the method, in the process of seedling transplanting and bud picking, the cut hard branch cuttings are inserted into a seedling raising tray, and after the cuttings root for one and a half months, the seedling transplanting is carried out. The transplanted seedlings generally grow 2-4 micro tips, redundant micro tips need to be erased in order to cultivate seedlings, only one micro tip is left to grow into seedlings, and the erased micro tips are thrown away as waste. Therefore, the hardwood cutting method still has the problem of low propagation coefficient.
In view of the above, there is a need for a method for raising sea-buckthorn seedlings by hardwood cutting with high propagation coefficient and high survival rate.
Disclosure of Invention
The invention aims to provide a sea-buckthorn hardwood cutting seedling method which is high in propagation coefficient and survival rate.
In order to realize the purpose, the adopted technical scheme is as follows:
a seabuckthorn hardwood cutting seedling method comprises the following steps:
(1) selecting cutting slips: selecting disease-free branches with thickness not less than 0.3cm and 1-2 years as cuttings in the tree dormancy stage;
(2) shearing treatment: cutting the cutting into 7-9cm long with parallel cut to obtain cutting slips;
(3) soaking treatment:
soaking the cutting in rooting water solution for 4-8h to a depth of 3-4cm to obtain soaked cutting;
(4) cuttage:
filling the matrix into holes of a seedling raising tray, trowelling and compacting, and then sprinkling until the matrix is wet; the substrate comprises field soil, decomposed organic fertilizer and perlite;
vertically cutting the soaked cutting slips into a wet substrate, wherein the cutting depth is 4.5-5.0 cm;
(5) water management: watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping watering 1-2 days before transplanting;
(6) transplanting and tending:
after 45-50 days of cuttage, ditching in a transplanting land, transplanting seedlings, performing nursery stock tending management after transplanting, and when the height of the seedlings is 40-60cm, taking out the seedlings for afforestation;
wherein, bud picking is started 7-10 days after transplanting, and only 1 bud tip is left to grow into seedlings;
(7) bud picking and micropropagation:
selecting bud tips with the length of 5-7cm from the removed bud tips, repeating the steps (3) - (5), cutting for 40-50 days, transplanting, overwintering in a nursery land, and enabling the seedling height to reach more than 70cm in the next year, so that the seedlings can be planted out of the nursery;
wherein the cutting depth is 2.5-3.5 cm;
and after the nursery stocks are transplanted, nursery stock tending management is carried out, and bud picking is carried out after 6 months of the next year.
Further, the step (1) further comprises the step of sand storing the cuttings after the cuttings are selected.
Further, in the step (2), when the temperature is lower than 20 ℃, the cutting shoots need to be stored in a moisture-preserving manner, and the cutting shoots can be reversely placed for root promotion in the storage process.
Further, in the step (3), in the rooting aqueous solution, the mass-to-volume ratio of rooting powder to water is 90-110 mg: 1L of the compound.
Further, in the rooting aqueous solution, the mass volume ratio of rooting powder to water is 100 mg: 1L;
the rooting powder is GGR rooting powder.
Further, in the step (3), the soaking time is 4 months to 5 months.
Further, in the step (4), the substrate is prepared according to the following steps: decomposed organic fertilizer: the perlite is mixed by the mass ratio of 4:0.8-1.2: 0.8-1.2.
Still further, the substrate is in accordance with the soil: decomposed organic fertilizer: the perlite is mixed according to the mass ratio of 4:1: 1.
Further, in the step (4), the aperture of the seedling raising tray is 45-50mm, and the length multiplied by the width multiplied by the height is 545mm multiplied by 280mm multiplied by 50 mm.
Further, in the step (6), the distance between the transplanted plants is 5.0 cm;
in the nursery stock tending and managing process: and 2-3 times of earthing up by shovel , and 1-2 times of topdressing.
Compared with the prior art, the invention has the advantages that:
1. according to the cutting seedling method for the hardwood of the sea buckthorn, the erased redundant useless shoot is subjected to cutting seedling again in the cutting seedling process of the hardwood of the sea buckthorn, the quantity of cutting materials is increased, the cutting coefficient is improved by 1-2 times, the cutting efficiency is improved by 2 times, the survival rate is high, the average survival rate of one-time cutting is more than 90%, the survival rate of secondary cutting, namely bud picking micropropagation can also reach more than 80%, and the yield of seedlings in unit area can reach 1.6-1.8 thousands of plants.
2. The method for raising sea-buckthorn hardwood cutting seedlings increases the utilization rate of cutting equipment, saves the cutting cost, improves the land utilization rate and increases the income.
3. Compared with the twig cutting seedling raising technology, the seabuckthorn hardwood cutting seedling raising method has the advantages that the cutting slips are thinner and shorter, the cutting conditions are simple and easy to implement, and time, labor and force are fully saved.
4. The method for raising the seedlings of the sea-buckthorn through hardwood cutting is simple, convenient and feasible, strong in operability and high in survival rate, and has important application value in expanding propagation and storing rare sea-buckthorn variety resources.
Drawings
FIG. 1 shows a bud picking micropropagation technique in the hardwood cutting seedling method of seabuckthorn according to the present invention.
Detailed Description
In order to further illustrate the hardwood cutting seedling raising method for sea buckthorn of the present invention to achieve the intended purpose of the invention, the following detailed description of the embodiments, structures, features and effects thereof will be made with reference to the preferred embodiments. In the following description, different "one embodiment" or "an embodiment" refers to not necessarily the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
Before explaining the method for raising sea-buckthorn seedlings by hardwood cutting in detail, it is necessary to further explain the raw materials and the method mentioned in the invention to achieve better effect.
In the process of raising sea-buckthorn hardwood cutting seedlings, the invention uses the unnecessary and useless bud tips removed as cutting slips to raise seedlings by cutting again, increases the propagation coefficient by 1-2 times on the basis of the original quantity of hardwood seedlings, and improves the seedling raising efficiency by more than 2 times. Solves the problems of low survival rate and low propagation coefficient of hardwood cutting.
The sand storage method is also called as low-temperature stratification, under the condition of low-temperature sand storage, fruits, vegetables or seeds enter a forced dormancy state temporarily, and a certain water content and oxygen content can maintain weak life activities. Particularly, the seeds are stored by a sand storage method, the permeability of seed coats is increased, the seeds can germinate when the temperature is proper in the next year, and the germination rate of the seeds is improved.
GGR rooting powder is a green plant growth regulator, is a non-hormone type physiological active substance with a renewal type, a broader spectrum and higher efficiency, can improve the survival rate of nursery stocks and the yield of plants, can make a positive response beneficial to the normal growth of the plants to the adverse environment stress, and reduces or avoids the damage to the plants caused by the adverse environment. It is easy to dissolve in water, can be stored at normal temp., and has no pollution and simple application method.
The shovel is used for shoveling weeds in the field of crops and loosening soil to ensure soil moisture.
Nursery stock tending management refers to measures taken for ensuring survival of young trees, promoting growth of trees, improving composition and quality of trees and improving forest productivity, and comprises weeding, loosening soil, fertilizing, watering, pruning, overwintering treatment and the like.
With the above materials and methods known, the method for raising sea buckthorn hardwood cutting seedlings according to the present invention will be described in further detail with reference to fig. 1 and the following specific examples:
example 1.
In this example, in 2016, the implementation in the market of seiuhuan in Heilongjiang province was carried out, the hard-shoot cuttage of the seabuckthorn variety "red in late autumn" was carried out, the survival rate of the first cuttage was 95%, and the survival rate of the second cuttage, i.e., the bud picking micropropagation, reached 88%, and the propagation coefficient was increased by 2 times.
With reference to fig. 1, the specific operation steps are as follows:
(1) selecting cutting slips: picking branches in the dormant period of a tree body, and selecting disease-free branches with the thickness not less than 0.3cm and growing for 1-2 years as cuttings in the middle and last ten days of autumn 10 months; after the cuttings are selected, the cuttings are subjected to sand storage, and the humidity of the cuttings is guaranteed.
(2) Early preparation:
firstly, a seedling raising tray: the specification of the seedling raising tray is 50 holes, the aperture is 45-50mm, the length multiplied by the width multiplied by the height is 545mm multiplied by 280mm multiplied by 50mm, and the preparation quantity is determined by the number of cuttings;
② matrix: the substrate was as follows (without herbicide) the soil: decomposed organic fertilizer: the perlite is prepared by uniformly mixing the perlite according to the mass ratio of 4:0.8: 1.2;
thirdly, water supply equipment: preparing a water drying pool and a water supply pump, paving water supply equipment by adopting micro-spraying or micro-spraying belts according to the wetting radius, and trying to spray before inserting, wherein the principle of ensuring that spraying is not exposed can be realized.
(3) Shearing treatment:
in the middle and last ten days of 4 months, cutting the cutting into 7-9cm long, and leveling the cut to obtain a cutting; and (4) temporarily preserving moisture and storing 50 spikes/bundle of cutting slips when the temperature does not reach 20 ℃, and reversely forcing roots during the storage period.
(4) Soaking treatment:
after the temperature reaches more than 20 ℃ from late ten days of 4 months to early 5 months, soaking the cutting slips in a rooting aqueous solution for 4 hours, wherein the soaking depth is 4 cm; wherein, in the rooting aqueous solution, the mass-volume ratio of GGR rooting powder to water is 110 mg: 1L of the compound.
(5) Cuttage:
spreading seedling raising trays, filling the prepared matrix into the holes, trowelling and compacting, placing in a field garden, and sprinkling until the matrix is wet;
and (3) after the cutting slips are soaked, vertically cutting the cutting slips into a wet substrate, wherein the cutting depth is 4.5-5.0 cm.
(6) Water management: watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping water supply 1 day before transplantation;
(7) transplanting and tending:
after 45 days of cuttage, ditching in a transplanting land with loose soil, transplanting the seedlings, wherein the transplanting distance is 5.0cm, and after transplanting, treading solid hilling and watering; then nursery stock tending management is carried out, and when the height of the nursery stock is 40-60cm, namely autumn, nursery can be carried out for afforestation;
wherein, bud picking is started 7 days after transplanting, only 1 bud tip is left to grow into seedlings, and redundant bud tips are removed;
nursery stock tending and managing: shovel soil 2 times, top application 1 time.
(8) Bud picking and micropropagation:
and selecting healthy shoot tips with the length of 5-7cm from the erased shoot tips as cutting slips for secondary cutting, and performing secondary cutting by using the erased shoot tips. Namely, repeating the steps (4) to (7) on the cutting equipment for one-time cutting, specifically:
before cutting, soaking the cutting slips subjected to secondary cutting in a rooting aqueous solution for 4h, wherein the soaking depth is 4 cm; wherein, in the rooting aqueous solution, the mass-volume ratio of GGR rooting powder to water is 110 mg: 1L;
after soaking treatment, vertically cutting into a wet substrate, wherein the cutting depth is 2.5-3.5 cm;
watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping water supply 1 day before transplantation;
after 50 days of cuttage, transplanting seedlings, then carrying out seedling tending management, safely overwintering, carrying out bud picking after 6 months of the next year, only leaving 1 bud tip to grow into seedlings, and removing redundant bud tips; when the height of the nursery stock is more than 70cm, namely autumn, the nursery stock can be taken out for afforestation;
wherein, in the annual nursery stock tending management: shovel soil 3 times, top application 1 time.
According to the method for raising seedlings of the sea-buckthorn hardwood by cutting, in the process of raising seedlings of the sea-buckthorn hardwood by cutting, the erased redundant useless shoot is raised by cutting again, so that the quantity of cutting materials is increased, the cutting coefficient is improved by 2 times, the cutting efficiency is improved, the survival rate is high, the survival rate of one-time cutting is 95%, and the survival rate of the second cutting can also reach more than 88%; in addition, the seedling raising method increases the utilization rate of the cuttage equipment and saves the cuttage cost; the seedling raising method is simple and easy to implement, strong in operability and high in survival rate, and has important application value for expanding propagation and storing rare sea-buckthorn variety resources.
Example 2.
In this example, in 2016, the black dragon river province implemented in the year 2015-.
The specific operation steps are as follows:
(1) selecting cutting slips: collecting cuttings in the dormant period of the tree body, and selecting 1-2 year-old disease-free cuttings with the thickness not less than 0.3cm in 3 months; after the cuttings are selected, the cuttings are subjected to sand storage, and the humidity of the cuttings is guaranteed.
(2) Early preparation:
firstly, a seedling raising tray: the specification of the seedling raising tray is 50 holes, the aperture is 45-50mm, the length multiplied by the width multiplied by the height is 545mm multiplied by 280mm multiplied by 50mm, and the preparation quantity is determined by the number of cuttings;
② matrix: the substrate was as follows (without herbicide) the soil: decomposed organic fertilizer: the perlite is mixed according to the mass ratio of 4:1.2: 0.8;
thirdly, water supply equipment: preparing a water drying pool and a water supply pump, paving water supply equipment by adopting micro-spraying or micro-spraying belts according to the wetting radius, and trying to spray before inserting, wherein the principle of ensuring that spraying is not exposed can be realized.
(3) Shearing treatment:
in the middle and last ten days of 4 months, cutting the cutting into 7-9cm long, and leveling the cut to obtain a cutting; as the temperature does not reach 20 ℃, 80-100 spikes/bundle of cutting slips are temporarily preserved for moisture preservation and storage, and the root can be promoted when the cutting slips are stored upside down.
(4) Soaking treatment:
after the temperature reaches more than 20 ℃ from late ten days of 4 months to early 5 months, soaking the cutting slips in a rooting aqueous solution for 8 hours, wherein the soaking depth is 3 cm; wherein, in the rooting aqueous solution, the mass volume ratio of GGR rooting powder to water is 90 mg: 1L of the compound.
(5) Cuttage:
spreading seedling raising trays, filling the prepared matrix into the holes, trowelling and compacting, placing in a field garden, and sprinkling until the matrix is wet;
and (3) after the cutting slips are soaked, vertically cutting the cutting slips into a wet substrate, wherein the cutting depth is 4.5-5.0 cm.
(6) Water management: watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping water supply 2 days before transplantation.
(7) Transplanting and tending:
after 50 days of cuttage, ditching in a transplanting land with loose soil, transplanting the seedlings, wherein the transplanting distance is 5.0cm, and after transplanting, treading solid and earthing up, and watering; then nursery stock tending management is carried out, and when the height of the nursery stock is 40-60cm, namely autumn, nursery can be carried out for afforestation;
wherein, bud picking is started after 10 days of transplanting, only 1 bud tip is left to grow into seedlings, and redundant bud tips are removed; nursery stock tending and managing: shovel soil 3 times, top application 2 times.
(8) Bud picking and micropropagation:
and selecting healthy shoot tips with the length of 5-7cm from the erased shoot tips as cutting slips for secondary cutting, and performing secondary cutting by using the erased shoot tips. Repeating the steps (4) to (7) on a cutting device for one-time cutting, wherein the height of the nursery stock in the next year can reach more than 70cm, and then outplanting and forestation can be carried out;
wherein, the cuttings obtained by the secondary cutting after the soaking treatment are vertically inserted into the wet substrate, and the cutting depth is 2.5-3.5 cm;
and (4) transplanting the seedlings after 45 days of cuttage, then carrying out nursery stock tending management, safely overwintering, and carrying out bud picking after 6 months of the next year.
According to the method for raising seedlings of the sea-buckthorn hardwood by cutting, in the process of raising seedlings of the sea-buckthorn hardwood by cutting, the erased redundant useless shoot is raised by cutting again, so that the quantity of cutting materials is increased, the cutting coefficient is improved by 1.5 times, the cutting efficiency is improved, the survival rate is high, the survival rate of one-time cutting is 96%, and the survival rate of secondary cutting, namely bud picking and micropropagation can also reach 82%; in addition, the seedling raising method increases the utilization rate of the cuttage equipment and saves the cuttage cost; the seedling raising method is simple and easy to implement, strong in operability and high in survival rate, and has important application value for expanding propagation and storing rare sea-buckthorn variety resources.
Example 3.
In this example, in 2016 + 2017, implemented in Heilongjiang province, hardwood cutting was performed on the seabuckthorn variety "red in late autumn", the survival rate of the first cutting was 93%, and the survival rate of the second cutting, i.e., bud picking and micropropagation, reached 85%, and the propagation coefficient was increased by 1.6 times.
The specific operation steps are as follows:
(1) selecting cutting slips: picking branches in the dormant period of the tree body, and selecting disease-free branches with the thickness not less than 0.3cm and 1-2 years as cuttings in the last ten days of 4 months; because the temperature reaches above 15 ℃, after the cutting is selected, the cutting can be directly sheared without sand storage.
(2) Early preparation:
firstly, a seedling raising tray: the specification of the seedling raising tray is 50 holes, the aperture is 45-50mm, the length multiplied by the width multiplied by the height is 545mm multiplied by 280mm multiplied by 50mm, and the preparation quantity is determined by the number of cuttings;
② matrix: the substrate was as follows (without herbicide) the soil: decomposed organic fertilizer: the perlite is mixed according to the mass ratio of 4:1: 1;
thirdly, water supply equipment: preparing a water drying pool and a water supply pump, paving water supply equipment by adopting micro-spraying or micro-spraying belts according to the wetting radius, and trying to spray before inserting, wherein the principle of ensuring that spraying is not exposed can be realized.
(3) Shearing treatment:
shearing the selected cutting into 7-9cm long, and leveling the shearing mouth to obtain a cutting slip; because the temperature reaches 20 ℃, the cutting slips do not need to be moisturized and stored and can be directly soaked.
(4) Soaking treatment:
soaking the cutting in rooting water solution for 6h to a depth of 3-4 cm; wherein, in the rooting aqueous solution, the mass volume ratio of GGR rooting powder to water is 100 mg: 1L of the compound.
(5) Cuttage:
spreading seedling raising trays, filling the prepared matrix into the holes, trowelling and compacting, placing in a field garden, and sprinkling until the matrix is wet;
and (3) after the cutting slips are soaked, vertically cutting the cutting slips into a wet substrate, wherein the cutting depth is 4.5-5.0 cm.
(6) Water management: watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping water supply 1 day before transplantation.
(7) Transplanting and tending:
after 48 days of cuttage, ditching in a transplanting land with loose soil, transplanting the seedlings, wherein the transplanting distance is 5.0cm, and after transplanting, treading solid for ridging and watering; then nursery stock tending management is carried out, and when the height of the nursery stock is 40-60cm, namely autumn, nursery can be carried out for afforestation;
wherein, bud picking is started after 8 days of transplanting, only 1 bud tip is left to grow into seedlings, and redundant bud tips are removed;
nursery stock tending and managing: shovel soil 3 times, top application 1 time.
(8) Bud picking and micropropagation:
and selecting healthy shoot tips with the length of 5-7cm from the erased shoot tips as cutting slips for secondary cutting, and performing secondary cutting by using the erased shoot tips. Repeating the steps (4) to (7) on a cutting device for one-time cutting, wherein the height of the nursery stock in the next year can reach more than 70cm, and then outplanting and forestation can be carried out;
wherein, the bud shoots after the soaking treatment are vertically inserted into the wet substrate, and the cutting depth is 2.5-3.5 cm;
and (4) transplanting the seedlings after 48 days of cuttage, then carrying out nursery stock tending management, safely overwintering, and carrying out bud picking after 6 months of the next year.
According to the method for raising seedlings of the sea-buckthorn hardwood by cutting, in the process of raising seedlings of the sea-buckthorn hardwood by cutting, the erased redundant useless shoot is raised again, the quantity of cutting materials is increased, the cutting coefficient is improved by 1.6 times, the cutting efficiency is improved, the survival rate is high, the survival rate of one-time cutting is 93%, and the survival rate of secondary cutting, namely bud picking and micropropagation can also reach 85%; in addition, the seedling raising method increases the utilization rate of the cuttage equipment and saves the cuttage cost; the seedling raising method is simple and easy to implement, strong in operability and high in survival rate, and has important application value for expanding propagation and storing rare sea-buckthorn variety resources.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (7)
1. A seabuckthorn hardwood cutting seedling method is characterized by comprising the following steps:
(1) selecting cutting slips: selecting disease-free branches with thickness not less than 0.3cm and 1-2 years as cuttings in the tree dormancy stage;
(2) shearing treatment: in the middle and last ten days of 4 months, cutting the cutting into 7-9cm long, and leveling the cut to obtain a cutting; when the temperature is lower than 20 ℃, the cutting slips need to be stored in a moisture-preserving way, and the cutting slips are inverted to promote root in the storage process;
(3) soaking treatment:
soaking the cutting in GGR rooting aqueous solution for 4-8h from the end of 4 months to the beginning of 5 months to obtain the soaked cutting, wherein the soaking depth is 3-4 cm;
(4) cuttage:
filling the matrix into holes of a seedling raising tray, trowelling and compacting, and then sprinkling until the matrix is wet; the matrix is prepared according to the following steps: decomposed organic fertilizer: the perlite is mixed by the mass ratio of 4:0.8-1.2: 0.8-1.2;
vertically cutting the soaked cutting slips into a wet substrate, wherein the cutting depth is 4.5-5.0 cm;
(5) water management: watering every morning or evening after cuttage, and watering 1-2 times every day until the matrix is wet; stopping watering 1-2 days before transplanting;
(6) transplanting and tending:
after 45-50 days of cuttage, ditching in a transplanting land, transplanting seedlings, performing nursery stock tending management after transplanting, and when the height of the seedlings is 40-60cm, taking out the seedlings for afforestation;
wherein, bud picking is started 7-10 days after transplanting, and only 1 bud tip is left to grow into seedlings;
(7) bud picking and micropropagation:
selecting bud tips with the length of 5-7cm from the removed bud tips, repeating the steps (3) - (5), cutting for 40-50 days, transplanting, overwintering in a nursery land, enabling the height of seedlings in the next year to be more than 70cm, and taking out of the nursery for forestation;
wherein the cutting depth is 2.5-3.5 cm;
and after the nursery stocks are transplanted, nursery stock tending management is carried out, and bud picking is carried out after 6 months of the next year.
2. The hardwood cutting seedling method of Hippophae rhamnoides according to claim 1, wherein,
and (2) selecting the cuttings, and then carrying out sand storage on the cuttings.
3. The hardwood cutting seedling method of Hippophae rhamnoides according to claim 1, wherein,
in the step (3), in the rooting aqueous solution, the mass-to-volume ratio of rooting powder to water is 90-110 mg: 1L of the compound.
4. The hardwood cutting seedling method of Hippophae rhamnoides of claim 3, wherein,
in the rooting aqueous solution, the mass volume ratio of rooting powder to water is 100 mg: 1L of the compound.
5. The hardwood cutting seedling method of Hippophae rhamnoides according to claim 1, wherein,
the matrix is prepared according to the following steps: decomposed organic fertilizer: the perlite is mixed according to the mass ratio of 4:1: 1.
6. The hardwood cutting seedling method of Hippophae rhamnoides according to claim 1, wherein,
in the step (4), the aperture of the seedling raising tray is 45-50mm, and the length multiplied by the width multiplied by the height is 545mm multiplied by 280mm multiplied by 50 mm.
7. The hardwood cutting seedling method of Hippophae rhamnoides according to claim 1, wherein,
in the step (6), the transplanting distance is 5.0 cm;
in the nursery stock tending and managing process: and 2-3 times of earthing up by shovel , and 1-2 times of topdressing.
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