CN109429853B

CN109429853B - Kiwi fruit green branch cutting seedling method

Info

Publication number: CN109429853B
Application number: CN201811188368.XA
Authority: CN
Inventors: 曾文静; 范晋铭; 周原
Original assignee: Sichuan Yinuoshi Agricultural Technology Co ltd
Current assignee: Sichuan Yinuoshi Agricultural Technology Co ltd
Priority date: 2018-10-12
Filing date: 2018-10-12
Publication date: 2021-04-02
Anticipated expiration: 2038-10-12
Also published as: CN109429853A

Abstract

The invention discloses a actinidia arguta green branch cutting seedling method, and relates to the technical field of actinidia arguta seedling; the invention comprises the following steps: peat and fine sand are used as seedbed matrixes, the seedbed matrixes are uniformly mixed to be made into a seedbed with the height of 5-8cm, and meanwhile, the seedbed is thoroughly watered with a prepared medicament solution; the medicament solution comprises 3000-4000ppm IBA (indolebutyric acid), 1000-2000ppm NAA (naphthylacetic acid) and 1000-fold 50 percent carbendazim; collecting branches with thickness of 0.2-0.4cm, cutting the collected branches into 2-4cm cuttings, cutting the upper cuts at 1-1.5cm above the bud eyes, reserving 1 leaf and cutting half of the leaf; inserting the branches into a seedbed, exposing the bud eyes on the surface of the substrate, and setting the cuttage density to 750-²(ii) a Controlling the temperature at 20-28 deg.C after cuttage, maintaining air humidity at 60-90% and Photosynthetically Active Radiation (PAR) at 10-50umol m^‑2s^‑1。

Description

Kiwi fruit green branch cutting seedling method

Technical Field

The invention relates to the technical field of actinidia arguta seedling culture, and particularly relates to a actinidia arguta green branch cutting seedling culture method.

Background

Kiwi fruit is a fruit product which is popular with consumers, has rich nutritive value, and can prevent and treat hypertension, cancer and other diseases. In order to realize high yield of the planted kiwi fruits, the kiwi fruit seedling culture work must be done.

The kiwi fruit seedling culture method comprises the following steps:

seed-growing seedling

And (5) collecting seeds. Selecting fully mature fruit of robust mother tree, softening, separating seed and pulp, cleaning, and drying in the shade.

And (4) seed treatment. Soaking the seeds dried in the shade in warm water at 45O ℃ for a day and night 40 days before sowing, padding wet sand (preferably just seeping water when being held tightly by hands) on the bottom of the flowerpot, then flatly placing the seeds, alternately storing the seeds in sand layers, and covering the seeds with felt.

And (6) sowing. Sowing in the next early spring, applying enough base fertilizer (6-7.5 tons of fertilizer per hectare) before sowing, irrigating enough water, and sowing seeds after the water is infiltrated. Because the kiwi fruit seeds are very small, the kiwi fruit seeds are preferably mixed by using screened nutrient soil for drilling, the row spacing is 15 cm, the seeding width is 20 cm, the seeding amount is 1 g/square meter, the screened nutrient soil is used for covering 2-3 mm after the seeding, and the straw is used for covering, so that the soil is kept moist frequently.

And (6) planting. And (3) timely uncovering the grass after seedling emergence, thinning and planting the seedlings when more than 3 true leaves grow out, wherein the seedling distance is 10-15 cm, transplanting the rest seedlings with soil for additional planting, applying thin fertilizer frequently in the seedling stage, and grafting when the diameter of the seedling reaches more than 0.5 cm.

Second, the grafted seedling is raised

And (5) grafting time. The optimal grafting period of the kiwi fruits is 6-7 months, and the survival rate is low in early and late.

A grafting method. The method of inserting skin and tongue grafting, cleft grafting, abdominal grafting, bud grafting and the like can be adopted, and the survival rate of bud grafting and inserting skin and tongue grafting is the highest.

And (5) management after grafting. After the grafted bud sprouts and new leaves are unfolded, the anvil is cut at the position of the joint which is more than 10 cm. And if the rootstock is cut too early, new strips are easy to be drawn from the rootstock to influence the development of the grafting bud. When the grafting is late, the stock can be cut after the sprouting in spring of the next year, and after the grafted sprout sprouts and the branches are taken out, the support posts are arranged for binding so as to avoid breaking.

Cutting seedling raising of three, hard branches

Cutting the cuttings: pruning in combination with dormant seasons for 2-3 months, pruning 1-year-old branches with the diameter of more than 0.8 cm from plants with more than 3 years, storing in an environment with the temperature of 0-7 ℃, and sealing and burying with warm sand.

Preparing a hotbed: the actinidia arguta hard wood cutting is difficult to root, and the root is promoted by heating in a hotbed. And (3) selecting dry and flat outdoor and indoor places at the beginning of 4 months, paving a foam plate with the thickness of 3cm, the length of 2 m and the width of 1.5 m on the ground surface, covering river sand with the thickness of 2cm on the foam plate, paving a ground heating line on the sand with the line distance of 5-6 cm, paving river sand with the thickness of 3cm on the line, and building the periphery of the sand firmly by using high wood plates or vertical bricks with the thickness of 10-12 cm.

Cutting and processing the cutting slips: taking out the cuttings, cutting the cuttings into spikes according to the length of 12-14 cm, wherein the distance between the top cutting opening of each spike and the top bud is 1-1.2 cm, the top bud needs to be full, the distance between the lower cutting opening and the lower end is 1.5cm, and the cutting opening is at an angle of 45 degrees. Binding every 25-50 ears into a bundle, upsetting the base part, and dipping the base part into indolebutyric acid solution with the concentration of 100-150 mg/kg for 24 hours by 2-3 cm.

Heating and root forcing: vertically placing the cutting shoots on a warm bed sand surface, firmly attaching the base ends to the sand surface, slightly leaving gaps between the bundles, wherein the ear placing amount is 2500-3000 ears/square meter, and the gaps are filled with fine sand, and the sand filling amount is that the terminal buds are 1cm higher than the sand. After the cutting is placed, the temperature is increased to accelerate the rooting, generally in the last ten days of 4 months, so that the temperature of the base part of the cutting is 25-28 ℃, but the indoor or outdoor temperature cannot be high, and is 5-7 ℃, and the sprouting and the rooting are inhibited. Spraying water to sand and ear frequently, and spraying 500-600 times of 50% carbendazim solution every 3-4 days for 1 time to prevent mildew and rot. After 15-20 days, stopping heating and water spraying when the root of the spike just forms 1 mm long root protomer, and preparing for transplanting after 2-3 days.

Transplanting cutting shoots: selecting field with flat terrain as transplanting field, wherein the soil texture is preferably sandy soil. Uniformly spreading farmyard manure 2-3 cubic meters per mu on the ground surface, turning the soil to 20 cm deep, and ridging according to the ridge distance of 60 cm and the ridge height of 20 cm. Transplanting can be carried out when the temperature reaches more than 10 ℃ in 15 cm deep soil on the local surface from late ten days of 4 months to early 5 months. In order to prevent the root of the cutting from being damaged, hard wooden sticks or bamboo sticks with the thickness of about 1cm are firstly inserted into the holes, the depth of the holes is about 10 cm, and 2 rows of holes are inserted into the surface of each ridge. And placing the cutting slips into the holes, enabling terminal buds to be 1.5cm higher than the ridge surface, and compacting.

Managing after broadcasting: and (5) irrigating enough water into the furrow and wetting the soil surface to 20 cm in depth. And (3) covering and pressing the ridge surface and the furrow by using a black mulching film for 2-3 days, and digging out film holes to expose the terminal buds of the cutting slips on the mulching film. Irrigation is needed in the growing season when severe drought occurs. If more than 2 young shoots are formed on the cutting, one upright shoot is selected at the position with the length of 10 cm and the rest shoots are removed. Weeds growing at the holes on the ridge surface are removed at any time. And killing the plants in time if leaves are exposed in 7-8 months. And (5) digging the agricultural film at the end of 10 months, digging the nursery stock, and placing the nursery stock in a cellar for sand storage or field temporary planting. If seedlings are to be raised in the spring of the next year, the seedlings are irrigated enough and the water is sealed, and the ridge surface is preferably covered with straw or corn stalks with the thickness of 20 centimeters to prevent the seedlings from being pulled out.

Twig cutting seedling

And (5) cutting period. The best period for cuttage of the tender branches is 6 middle-month and 9 middle-month, and the high-temperature weather in summer should be avoided after the growth peak of the kiwi fruit.

And (4) selecting and processing the cutting slips. Selecting semi-lignified branches of the current year without diseases and insect pests as cuttings, reserving 2-3 buds on each branch, reserving 1-2 leaves on the upper part, cutting half of the reserved leaves, carrying out horizontal shearing or oblique shearing on the lower cut of the cuttings close to internodes, wherein the cut must be smooth, and then soaking the cut in rooting powder treatment solution.

Preparing the slotting machine and managing after the slotting. The slotting bed should be selected in the place with good water source, the screened sandy soil is used as bed soil, and the zineb or potassium permanganate is used for soil disinfection, and a shading shed with the height of 2 meters is built for shading after the land is leveled. Checking at any time after cutting to keep the humidity in the shed at 90%, spraying water once in the morning and afternoon of a sunny day, cutting the cuttings to root after 20 days, spraying 0.2% urea or potassium dihydrogen phosphate after 25 days to perform topdressing and strengthening seedlings outside the roots, and transplanting after about 40 days of cutting.

Root-cutting seedling raising

The root tillering force of the kiwi fruit is strong, when the seedling is lifted or the land is prepared, the excavated root segment promotes the sprouting of the stock seedling, and a large amount of seeds and branches can be saved. The method comprises the steps of cutting a root with a pencil thickness into root sections of 10-15 cm, cutting with a big head upwards and a small head downwards, cutting the small head flatly by using pruning shears, germinating after cutting, growing into plants, cutting into roots with roots in the first 4 months and the middle ten days to obtain a high survival rate, preparing a cutting bed and managing the same tender branch cutting after cutting.

With the development of the planting industry, the cuttage breeding technology has become a widely used method for breeding and storing excellent plants of fruit trees. At present, actinidia arguta is used as a rare species, and a large amount of breeding methods are needed urgently, wherein the cutting seedling raising is an ideal method which is low in cost, simple to operate and capable of keeping female parent excellent properties. However, the existing soft jujube green branch cuttage technology is less in research, and the green branch cuttage breeding technology in the prior art is not high in practicability in a high-temperature and high-humidity environment in the south, so that the technology cannot be applied commercially.

Disclosure of Invention

The invention aims to: the invention provides a actinidia arguta cutting seedling method suitable for a high-temperature and high-humidity environment in southern areas.

The invention specifically adopts the following technical scheme for realizing the purpose:

a actinidia arguta green branch cutting seedling method comprises the following steps:

firstly, preparing a seedbed:

peat and fine sand are used as substrates, the peat and the fine sand are uniformly mixed to prepare a seedbed with the height of 5-8cm, and the seedbed is thoroughly watered with a prepared medicament solution;

the pesticide solution comprises 3000-4000ppm IBA (indolebutyric acid), 1000-2000ppm NAA (naphthylacetic acid) and 1000 times of 50% carbendazim, wherein the carbendazim is a broad-spectrum bactericide and has a control effect on diseases of various crops caused by fungi (such as fungi imperfecti and ascomycetes); can be used for foliar spraying, seed treatment and soil treatment, and can effectively prevent and treat various crop diseases caused by fungi. Indolebutyric acid is mainly used for cutting rooting agent, can be used for flushing application, drip irrigation, flushing fertilizer synergist, foliar fertilizer synergist, plant growth regulator, can induce the formation of root protomer, is used for cell division and cell proliferation, is favorable to the new root to produce and the differentiation of vascular bundle system, promote the formation of adventitious root of cutting, promote the meristem of root of herbaceous and woody plant, the cutting soaked in naphthylacetic acid solution can promote the rooting, improve the survival rate of cuttage;

(II) collection of cuttings: collecting branches with the thickness of 0.2-0.4cm, cutting the collected branches into cuttings with the thickness of 2-4cm, cutting an upper opening at a position 1-1.5cm above bud eyes, reserving 1 leaf and reducing half of the leaf, wherein the bud eyes are pits with buds hidden on the surface, the bud eyes are generally spirally arranged, each bud eye is generally provided with 2-3 axillary buds, 1 axillary bud can develop, and the probability of the axillary bud at the bud eye of each cutting to grow into a seedling is increased.

(III) branch cuttage: inserting the branches into a seedbed, exposing the surface of the substrate with bud eyes, and ensuring that the cuttage density is about 750-²And the bud eyes are exposed out of the surface of the substrate, so that axillary buds in the bud eyes can be irradiated by sunlight.

(IV) post-management: controlling the temperature at 20-28 deg.C, maintaining air humidity at 60-90% and Photosynthetic Active Radiation (PAR) at 10-50umol m within one week after cuttage^-2s^-1And after the cuttings take roots and bud, applying a high-nitrogen water-soluble fertilizer.

Further, in the preparation of the seedbed in the step (I), the height of the seedbed is 6-7 cm; the medicament solution comprises 3300-3700ppm IBA (indolebutyric acid), 1300-1700ppm NAA (naphthylacetic acid) and 1000 times of 50% carbendazim;

in the step (II), in the collection of the cuttings, the thickness of the collected branches is 0.3-0.4cm, the collected branches are cut into cuttings of 3-4cm, and the upper cuts are positioned at 1.5cm positions above the bud eyes;

in the step (three), the cuttage density is 750-²；

In the later management of the step (IV), the temperature is controlled to be 24-28 ℃, the air humidity is kept at 80-90 percent and the Photosynthetically Active Radiation (PAR) is kept at 10-30umol m within one week after cuttage^-2s^-1。

Further, in the step (a) of bed preparation, the height of the bed is 7cm, and the solution of the agent comprises 4000ppm of IBA (indolebutyric acid), 2000ppm of NAA (naphthylacetic acid) and 1000 times of 50% carbendazim;

in the step (II), in the collection of the cuttings, the thickness of the collected branches is 0.3cm, the collected branches are cut into cuttings of 3cm, and the upper cuts are 1.5cm above the bud eyes;

in the step (III), the cuttage density is 800 plants/m²；

In the later management of the step (IV), the temperature is controlled to be 24 ℃, the air humidity is kept at 80 percent, and the Photosynthetically Active Radiation (PAR) is kept at 20umol m within one week after cuttage^-2s^-1。

The invention has the following beneficial effects:

1. the invention adopts the green branch cutting seedling raising technology to shorten the seedling time of the actinidia arguta tissue culture seedlings. The green branch cuttage is carried out in a greenhouse, the growth speed is high under the appropriate environmental condition, seedlings can be grown in about 20 days, the rooting rate is up to 90%, the seedling growing period is greatly shortened, the rooting rate is high, the economic loss of the seedling growing is reduced by about 30%, the economic benefit is increased for enterprises, and the seedlings are grown in 30 days, the seedling growing rate is high in 4-8 months per year, the technical bottleneck of the commercialization degree of the actinidia arguta seedling growing in the high-temperature high-humidity environment in the south by the traditional seedling growing mode is broken, and the defects of the seedling growing of the actinidia arguta in the low-tissue culture seedlings of the actinidia arguta are that the plant characters are separated and the seedling growing time is long; because the grafted seedlings and the root-cutting seedlings are high in cost and long in seedling growing period, and are used for cultivating the actinidia arguta, commercialization and specialization are not realized on the actinidia arguta, and the survival rate of the seedlings can be reduced due to improper timing of the hard-branch cutting seedlings and the seedling growing of the seeds, so that the seedling growing time is too long, the seedling growing risk is too large, and the method is not suitable for commercial production; the seedling raising environment of the green branch cutting seedling raising technology adopted by the invention is low in cost and high in seedling raising survival rate.

2. According to the invention, a matrix irrigation mode is adopted, the prepared medicament solution is irrigated through the seedbed, compared with the traditional mode of dipping the root-inducing powder on the branches, the labor cost can be greatly reduced, for example, each person dips the root-inducing powder once per second, 6000 branches need to be dipped by one person for about 100 minutes and need to work without gaps, and the irrigation of the 6000 branches can be completed in only 10 minutes or even several minutes by adopting an irrigation mode, so that the efficiency can be improved and the labor force can be saved by adopting the matrix irrigation mode.

3. Seedling raising technology by cutting green branches and seedbed cuttage thereofThe insertion density is as high as 800 plants/m²Only 10m is needed for cultivating 8000 seedlings²Greatly saving space and improving land utilization rate.

4. Seedling raising costs of seedling raising, grafting and root cutting seedling raising of the seeds in the actinidia arguta tissue culture seedlings are high, the survival rate of hardwood cutting seedling raising is low, proper seedling raising time sections need to be selected for seedling raising of the seedlings, seedling raising survival rates can be reduced if the seedling raising selection time is not proper, seedling raising time is too long, seedling raising risks are too large, and the actinidia arguta tissue culture seedlings are not suitable for commercial production; the seedling raising environment of the green branch cutting seedling raising technology adopted by the invention is low in cost and high in seedling raising survival rate.

Drawings

FIG. 1 is a schematic view of the present invention with cuttings being inserted in a seedbed.

Reference numerals: 1-seedbed, 2-cuttings.

Detailed Description

In order that those skilled in the art will better understand the present invention, the following examples are provided to illustrate the present invention in further detail. The following detailed description of embodiments of the invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The embodiment provides a actinidia arguta green branch cutting seedling method, which comprises the following steps:

firstly, preparing a seedbed 1:

peat and fine sand are used as substrates, the peat and the fine sand are mixed uniformly to form a seedbed 1 with the height of 5cm, and meanwhile, the seedbed 1 is watered thoroughly by using prepared medicament solution.

The pharmaceutical solution comprises 3000ppm IBA (indolebutyric acid), 1000ppm NAA (naphthylacetic acid) and 1000 times 50% carbendazim. Carbendazim is a broad-spectrum bactericide and has control effect on diseases of various crops caused by fungi (such as adelomycetes and ascomycetes). Can be used for foliar spraying, seed treatment and soil treatment, and can effectively prevent and treat various crop diseases caused by fungi. Indolebutyric acid is mainly used as rooting agent of cutting 2, can be used for irrigation, drip irrigation, irrigation fertilizer synergist, foliar fertilizer synergist and plant growth regulator, can induce the formation of root protomer, is used for cell division and cell proliferation, is beneficial to the generation of new roots and the differentiation of vascular bundle systems, promotes the formation of adventitious roots of cutting 2 and promotes the meristem of roots of herbaceous and woody plants. The cutting 2 soaked in the naphthylacetic acid solution can promote rooting and improve the survival rate of cuttage.

(II) collecting the cuttings 2: collecting branches with thickness of 0.2-0.4cm, cutting the collected branches into 2cm cuttings 2, cutting the upper cuts at 1cm above the bud eyes, reserving 1 leaf and reducing half of the leaf. The bud eyes are sunken positions with buds hidden on the surface, the bud eyes are generally spirally arranged, each bud eye is generally internally provided with 2-3 axillary buds, wherein 1 axillary bud can develop, and the probability of the axillary bud at the bud eye position of the cutting 2 is increased.

(III) branch cuttage: inserting the branches into a seedbed 1, exposing bud eyes on the surface of the substrate, and ensuring the cuttage density to be about 750 plants/m². The bud eyes are exposed out of the surface of the substrate, so that axillary buds in the bud eyes can be irradiated by sunlight.

(IV) post-management: controlling the temperature at 20 deg.C, maintaining the air humidity at 60%, and maintaining the Photosynthetically Active Radiation (PAR) at 10umol m within one week after cuttage^-2s^-1And after the cuttings root, applying high-nitrogen water-soluble fertilizer.

Example 2

firstly, preparing a seedbed 1:

peat, fine sand and perlite are used as substrates, the peat, the fine sand and the perlite are uniformly mixed to prepare a seedbed 1 with the height of 8cm, and meanwhile, the seedbed 1 is thoroughly watered with a prepared medicament solution.

The pharmaceutical solution comprises 4000ppm IBA (indolebutyric acid), 2000ppm NAA (naphthylacetic acid) and 1000 times 50% carbendazim. Carbendazim is a broad-spectrum bactericide and has control effect on diseases of various crops caused by fungi (such as adelomycetes and ascomycetes). Can be used for foliar spraying, seed treatment and soil treatment, and can effectively prevent and treat various crop diseases caused by fungi. Indolebutyric acid is mainly used as a cutting rooting agent, can be used for irrigation application, drip irrigation, irrigation fertilizer synergist, foliar fertilizer synergist and plant growth regulator, can induce the formation of root protomer, is used for cell division and cell proliferation, is beneficial to the generation of new roots and the differentiation of vascular bundle systems, promotes the formation of adventitious roots of cutting and promotes the meristem of the roots of herbaceous and woody plants. The cutting slips soaked in the naphthylacetic acid solution can promote rooting and improve the survival rate of cutting.

(II) collecting the cuttings 2: collecting branches with thickness of 0.2-0.4cm, cutting the collected branches into 4cm cuttings, making upper cuts at 1.5cm above the eyes, retaining 1 leaf and cutting half of the leaf. The bud eyes are sunken positions with buds hidden on the surfaces, the bud eyes are generally arranged in a spiral shape, 2-3 axillary buds are generally arranged in each bud eye, 1 axillary bud can grow, and the probability of the axillary bud at the bud eye position of the cutting is increased.

(III) branch cuttage: inserting the branches into a seedbed 1, exposing bud eyes on the surface of the substrate, and ensuring the cuttage density to be about 850 plants/m². The bud eyes are exposed out of the surface of the substrate, so that axillary buds in the bud eyes can be irradiated by sunlight.

(IV) post-management: controlling the temperature at 28 deg.C, maintaining the air humidity at 90% and the Photosynthetically Active Radiation (PAR) at 30umol m within one week after cuttage^-2s^-1And after the cuttings take roots and bud, applying a high-nitrogen water-soluble fertilizer.

Example 3

firstly, preparing a seedbed 1:

peat, fine sand and perlite are used as substrates, the peat, the fine sand and the perlite are uniformly mixed to prepare a seedbed 1 with the height of 6.5cm, and meanwhile, the seedbed 1 is thoroughly watered with a prepared medicament solution;

the medicament solution comprises 3500ppm IBA (indolebutyric acid), 1500ppm NAA (naphthalene acetic acid) and 1000 times of 50% carbendazim.

(II) collecting the cuttings 2: collecting branches with thickness of 0.2-0.4cm, cutting the collected branches into 3cm cuttings, making upper cuts at 1.5cm above the eyes, retaining 1 leaf and cutting half of the leaf.

(III) branch cuttage: inserting the branches into a seedbed, exposing bud eyes on the surface of the substrate, and ensuring the cuttage density to be 800 plants/m²。

(IV) post-management: controlling the temperature at 26 deg.C, maintaining the air humidity at 80%, and maintaining the Photosynthetically Active Radiation (PAR) at 20umol m within one week after cuttage^-2s^-1And after rooting and sprouting, applying high-nitrogen water-soluble fertilizer. Watering at irregular period to keep the matrix moist, removing necrotic branches and leaves in time, gradually increasing photosynthetically active radiation after one week, spraying bactericide if necessary, and watering NPK30-10-10 water soluble fertilizer (high nitrogen water soluble fertilizer NPK30-10-10)1000 times night after the cuttings root. After about 20 days, the cuttings sprout and take root, and then can be transplanted.

The test results of the test using the method of this example with the green shoot cuttings of 800 branches are as follows (wherein the number of the control group selected is the same as the green shoot cuttings of 800 branches, and the control group is the traditional green shoot cutting method):

	experimental group	Control group
			Number of roots	723	568
Rooting rate	90.4％	51.2％
			Days of rooting	22 days	25 days

Table 1(6 months experiment comparison table)

Month of the year	Number of roots	Rooting rate	Days of rooting
				4 month	692	86.5％	28 days
Month 5	721	90.1％	22 days
				6 month	723	90.4％	22 days
7 month	718	89.8％	22 days
				8 month	708	88.5％	26 days

As can be seen from the comparison in Table 1, the present invention adopts the green branch cutting seedling raising technology to shorten the seedling time of the actinidia arguta tissue culture seedling. The green branch cuttage is carried out in a greenhouse, cuttage can be carried out all the year round under the appropriate environmental conditions, the growth speed is high, seedlings can grow within about 20 days, the rooting rate is up to more than 90%, the seedling growing period is greatly shortened, the rooting rate is high, the economic loss of the seedling growing is reduced by about 30%, the economic benefit is increased for an enterprise, the seedlings can grow into 5 batches every year in a period of 30 days, and the annual seedling growing of 5 batches for the enterprise can bring greater economic income for the enterprise, so that the actinidia arguta seedlings are continuously produced.

As can be seen from table 2, the actinidia arguta green branch cutting seedling raising method has a high rooting rate in 4 to 8 months per year, breaks through the technical bottleneck that the commercialization degree of actinidia arguta seedling raising in the high-temperature and high-humidity environment in the south of the traditional seedling raising method is not high, and has the defects that the plant character separation is realized and the seedling raising time is long in the actinidia arguta tissue culture seedling; because the grafted seedlings and the root-cutting seedlings are high in cost and long in seedling growing period, and are used for cultivating the actinidia arguta, commercialization and specialization are not realized on the actinidia arguta, and the survival rate of the seedlings can be reduced due to improper timing of the hard-branch cutting seedlings and the seedling growing of the seeds, so that the seedling growing time is too long, the seedling growing risk is too large, and the method is not suitable for commercial production; the seedling raising environment of the green branch cutting seedling raising technology adopted by the invention is low in cost and high in seedling raising survival rate.

The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention, and the scope of the present invention is defined by the appended claims, and all changes that come within the meaning and range of equivalency of the specification are therefore intended to be embraced therein.

Claims

1. A actinidia arguta green branch cutting seedling method is characterized by comprising the following steps:

the method comprises the following steps:

firstly, preparing a seedbed:

peat and fine sand are used as a seedbed substrate, the seedbed is prepared into a seedbed with the height of 5-8cm after being uniformly mixed, and a prepared reagent solution is used for pouring the seedbed thoroughly, wherein the reagent solution comprises 3000-4000ppm IBA (indolebutyric acid), 1000-2000ppm NAA (naphthylacetic acid) and 1000 times of 50 percent carbendazim;

(II) collection of cuttings: collecting branches with thickness of 0.2-0.4cm, cutting the collected branches into 2-4cm cuttings, cutting the upper cuts at 1-1.5cm above the bud eyes, reserving 1 leaf and cutting half of the leaf;

(III) branch cuttage: inserting the branches into a seedbed, exposing the bud eyes on the surface of the substrate, and setting the cuttage density to 750-²；

(IV) post-management: controlling the temperature at 20-28 deg.C, maintaining air humidity at 60-90% and Photosynthetic Active Radiation (PAR) at 10-30umol m within one week after cuttage^-2s^-1And after the cuttings take roots, applying high-nitrogen water-soluble fertilizer.

2. The actinidia arguta green branch cutting seedling method according to claim 1, characterized in that:

in the step (I), the height of the seedbed is 6-7cm in the preparation of the seedbed; the medicament solution comprises 3300-3700ppm IBA (indolebutyric acid), 1300-1700ppm NAA (naphthylacetic acid) and 1000 times of 50% carbendazim,

in the step (II), in the collection of the cuttings, the thickness of the collected branches is 0.2-0.4cm, the collected branches are cut into cuttings of 3-4cm, and the upper cuts are positioned at 1.5cm positions above the bud eyes;

in the step (three), the cuttage density is 750-²；

3. The actinidia arguta green branch cutting seedling method according to claim 1, characterized in that:

in the step (I), in the preparation of the seedbed, the height of the seedbed is 7cm, and the medicament solution comprises 4000ppm of IBA (indolebutyric acid), 2000ppm of NAA (naphthylacetic acid) and 1000 times of 50% carbendazim;

in the step (II), in the collection of the cuttings, the thickness of the collected branches is 0.2-0.4cm, the collected branches are cut into cuttings of 3cm, and the upper cuts are 1.5cm above the bud eyes;

in the step (III), the cuttage density is 800 plants/m²；