CN111527898A - Method for cutting and root-forcing of sea-buckthorn hardwood - Google Patents

Method for cutting and root-forcing of sea-buckthorn hardwood Download PDF

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Publication number
CN111527898A
CN111527898A CN202010546075.5A CN202010546075A CN111527898A CN 111527898 A CN111527898 A CN 111527898A CN 202010546075 A CN202010546075 A CN 202010546075A CN 111527898 A CN111527898 A CN 111527898A
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CN
China
Prior art keywords
cutting
cuttings
slips
hardwood
root
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Pending
Application number
CN202010546075.5A
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Chinese (zh)
Inventor
张东为
王洪江
戈素芬
张海旺
赵旭珍
王占龙
任丽华
付庆新
郑宇超
卢立华
佟景春
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Liaoning Institute Of Dryland Agriculture And Forestry
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Liaoning Institute Of Dryland Agriculture And Forestry
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Publication date
Application filed by Liaoning Institute Of Dryland Agriculture And Forestry filed Critical Liaoning Institute Of Dryland Agriculture And Forestry
Priority to CN202010546075.5A priority Critical patent/CN111527898A/en
Publication of CN111527898A publication Critical patent/CN111527898A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18Liquid substances or solutions comprising solids or dissolved gases

Abstract

The invention relates to the technical field of seabuckthorn hardwood cutting, in particular to a seabuckthorn hardwood cutting root-forcing method, which comprises the following steps: s1, constructing an indoor slotting machine: laying electric heating wires indoors, connecting with an automatic temperature control instrument to form a slotting machine; s2, collection of cutting shoots: collecting cuttings which are strong and do not break and have the diameter of more than or equal to 0.5cm and grow for 1-2 years from the sea buckthorn trees 2-3 weeks before the sea buckthorn sprouts in winter or spring; s3, cutting: cutting off the cuttings, bundling the cuttings and placing the cuttings into a cellar for preservation; s4, cutting and root forcing: taking out the bundled cutting slips for root promotion, and vertically arranging the bundled cutting slips in a cutting bed; s5, post-insertion processing: after the cutting is processed, the constant temperature and humidity of the seedbed are controlled, and the cutting obtained with the callus is transplanted into a nutrition cup for cultivating seedlings. The invention promotes the callus generation and rooting speed of the sea-buckthorn hard branch cutting slips, improves the rooting rate and survival rate of the cutting slips, and provides a new technical approach for quick breeding and seedling raising of sea-buckthorn hard branches.

Description

Method for cutting and root-forcing of sea-buckthorn hardwood
Technical Field
The invention relates to the technical field of seabuckthorn hardwood cutting, in particular to a seabuckthorn hardwood cutting root-forcing method.
Background
The cutting propagation of sea-buckthorn generally adopts hardwood cutting and twig cutting. The twig cuttage is easy to take root, the propagation coefficient is high, and the method is widely applied to production. However, as the factors influencing rooting by the twig cutting are more, the management is more complicated and the requirements are strict in the breeding process, most of the seedlings bred by the twig cutting need to be transplanted for 1.5-2.0 years, so that the seedlings can be outplanted, and the time is long. But the survival rate and the propagation coefficient of the hardwood cutting are low, the use amount in the production is less, and the method is only applied to a few varieties which are easy to root.
In the existing root forcing technology, a composite rooting solution formed by mixing 75ppm NAA and 150ppm IBA is usually used for improving the root forcing rate, but the root forcing time of the mode is longer, and in addition, the rooting rate of the sea buckthorn hardwood cutting in the prior art is lower, and the survival rate is lower.
Therefore, a method for cutting and root-forcing sea-buckthorn hardwood branches is provided for solving the problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for cutting and root-forcing of hardwood of sea buckthorn.
A seabuckthorn hardwood cutting root-forcing method comprises the following steps:
s1, constructing an indoor slotting machine: laying an electric heating wire indoors, covering the electric heating wire with disinfected sawdust, connecting an automatic temperature control instrument, inserting a temperature sensing head of the temperature control instrument in the middle of the cutting bed, enabling the bottom of the temperature sensing head to be parallel to the base of the cutting slips, and flattening the bed surface to form the cutting bed;
s2, collection of cutting shoots: collecting cuttings which are strong and do not break and have the diameter of more than or equal to 0.5cm and grow for 1-2 years from the sea buckthorn trees 2-3 weeks before the sea buckthorn sprouts in winter or spring;
s3, cutting: cutting the cuttings to obtain cuttings, bundling every 30-50 cuttings into a bundle, placing the bundle in a cellar, and covering and storing the bundle with wet sand;
s4, cutting and root forcing: taking out the bundled cutting slips, quickly dipping the bottoms of the bundled cutting slips for 10s by using ABT1 rooting powder, taking the upper parts of the processed cutting slips, enabling the bottoms of the cutting slips to face downwards, twisting the cutting slips left and right in wet saw dust to enable the wet saw dust to completely fill gaps at the bottoms of the cutting slips, then vertically arranging the bundled cutting slips in a cutting bed, filling saw dust between the cutting slips to reach the thickness of 2/3 with the length of cutting slips, and reserving 2-3 buds at the tops of the cutting slips;
s5, post-insertion processing: after the cutting is processed, the constant temperature and humidity of the seedbed are controlled, water is timely supplemented when the humidity is insufficient, 500 times of carbendazim liquid medicine is sprayed once a week after root forcing is carried out for 7-8 days, after cutting is carried out for 10-15 days, a large number of callus tissues are formed at the base part of the cutting, a large number of root systems are generated, and the cutting obtained the callus tissues is transplanted to a nutrition cup or a field for cultivating seedlings.
Preferably, the line distance between the adjacent electric heating wires in the S1 is 10cm, the electric heating wires are arranged in parallel, and the thickness of the sawdust is 8cm-10 cm.
Preferably, the sawdust disinfection method in S1 is as follows: spraying 0.2-0.3% potassium permanganate solution onto sawdust 1-2 days before cuttage, with a spraying amount of 3g/m2And fully leaching the sawdust for 3-4 hours by using clean water 24 hours after spraying.
Preferably, when the cuttings are cut in the S3, the upper ends of the cuttings are cut horizontally, the lower ends of the cuttings are cut horizontally or obliquely, and the humidity of the sand in the S3 is more than or equal to 75%.
Preferably, the length of the cuttings in S3 is 8cm-12 cm, and the diameter is more than or equal to 0.5 cm.
Preferably, the preparation process of the ABT1 rooting powder in S4 comprises the following steps: 1g of rooting powder is taken, dissolved by 95 percent of alcohol and then is added with distilled water to reach the constant volume of 1L.
Preferably, the temperature of the seedbed in the S5 is controlled to be 23-27 ℃, and the relative humidity is controlled to be 70-85%.
Preferably, the callus formation rate at the base of the ear in S5 is not less than 85%.
The invention has the beneficial effects that:
1. the invention adopts the electric heating mode to promote the callus generation and rooting speed of the sea-buckthorn hardwood cutting slips, improve the rooting rate and survival rate of the cutting slips and provide a new technical approach for the rapid propagation and seedling culture of the sea-buckthorn hardwood by arranging the electric heating temperature control cutting bed, controlling the temperature of the cutting slips to be about 25 ℃ and controlling the relative humidity to be 70-85%.
2. The invention quickly dips the cutting slips with ABT1 rooting powder, the rooting powder is dissolved with 95% alcohol before the rooting powder is dissolved, and then the volume is determined to 1L by distilled water, thereby greatly accelerating the callus generation and rooting speed of the sea-buckthorn hard branch cutting slips.
3. The invention uses potassium permanganate solution to spray and disinfect before covering the sawdust, thereby ensuring the safety of the sawdust and improving the sterility of the cutting after insertion.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
A seabuckthorn hardwood cutting root-forcing method comprises the following steps:
s1, constructing an indoor slotting machine: laying electric heating wires at a line distance of 10cm in parallel indoors, covering the electric heating wires with disinfected sawdust with a thickness of 10cm, connecting an automatic temperature control instrument, inserting a temperature sensing head of the temperature control instrument in the middle of the cutting bed, enabling the bottom of the temperature sensing head to be parallel to the base of the cutting slips, and flattening the bed surface to form the cutting bed;
s2, collection of cutting shoots: collecting cuttings which are strong in growth for 1 year, are not damaged and have the diameter of more than or equal to 0.5cm from a sea buckthorn female tree within half a month before sea buckthorn germinates in winter;
s3, cutting: cutting the upper end of the cut of the cutting when cutting the cutting, obliquely cutting the lower end, wherein the length of the cutting after cutting is 10cm, the diameter is 0.7cm, bundling every 40 cut cutting into a bundle, placing the bundle into a cellar, and covering and storing the bundle with wet sand (the humidity of the sand is kept above 75%);
s4, cutting and root forcing: taking out the bundled cutting slips, quickly dipping the bottoms of the bundled cutting slips for 10s by using ABT1 rooting powder, taking the upper parts of the processed cutting slips, enabling the bottoms of the cutting slips to face downwards, twisting the cutting slips left and right in wet saw dust to enable the wet saw dust to completely fill gaps at the bottoms of the cutting slips, then vertically arranging the bundled cutting slips in a cutting bed, filling saw dust between the cutting slips to reach the thickness of 2/3 of the length of cutting slips, and reserving 3 buds at the tops of the cutting slips;
s5, post-insertion processing: after the cutting is processed, the temperature of a seedbed is controlled to be kept at 25 ℃, the relative humidity is kept at 76%, water is timely supplemented when the humidity is insufficient, 700 times of carbendazim liquid medicine is sprayed once a week after root forcing for one week, after half a month of cutting, a large number of calluses are formed at the base parts of the cutting and a large number of root systems are generated, and the cutting obtained the calluses is transplanted into a nutrition cup to be used for cultivating seedlings.
The sawdust disinfection method in the S1 comprises the following steps: spraying 0.3% potassium permanganate solution onto sawdust two days before cuttage, with a spraying amount of 3g/m2Fully leaching the sawdust with clear water for 4 hours after spraying; the preparation process of ABT1 rooting powder in S4 is as follows: 1g of rooting powder is taken, dissolved by 95 percent of alcohol and then is added with distilled water to reach the constant volume of 1L.
The first embodiment is as follows:
firstly, collecting 1-year-old cuttings in half a month before the germination of sea buckthorn in winter, cutting the cuttings into cuttings with the length of 10cm and the diameter of 0.7cm, and bundling every 40 cuttings and placing the cuttings in a cellar and storing the cuttings with wet sand;
then, laying electric heating wires in parallel at a wire pitch of 10cm indoors, covering the electric heating wires with disinfected sawdust, connecting an automatic temperature control instrument, inserting a temperature sensing head of the temperature control instrument in the middle of the cutting bed, enabling the bottom of the temperature sensing head to be parallel to the base of the cutting slips, and flattening the bed surface to form the cutting bed;
then, during cutting, firstly taking out a bundle of cutting slips, quickly dipping the bottom of each bundle of cutting slips in ABT1 rooting powder for 10s, then downwards turning the bottom of each cutting slip, twisting the wet cutting slips left and right in the wet cutting slips to enable the wet cutting slips to be completely filled in gaps at the bottom of the cutting slips, and then placing the bundle of cutting slips in a cutting bed side by side, wherein the wet cutting slips are filled in the gaps;
then, after cutting, the temperature of the electric heating hotbed is controlled at 25 ℃, the relative humidity is kept at 76%, water should be supplemented in time when the humidity is insufficient, 700 times of carbendazim liquid medicine is sprayed once a week after root forcing for one week, after half a month of cutting, a large amount of callus tissues are formed at the base parts of the cutting slips, a large amount of root systems are generated, and the cutting slips with the callus tissues obtained are transplanted into a nutrition cup for cultivating seedlings.
Example two (taking a composite rooting solution of 75ppm NAA and 150ppm IBA):
firstly, collecting 1-year-old cuttings in half a month before the germination of sea buckthorn in winter, cutting the cuttings into cuttings with the length of 10cm and the diameter of 0.7cm, and bundling every 40 cuttings and placing the cuttings in a cellar and storing the cuttings with wet sand;
then, laying electric heating wires in parallel at a wire pitch of 10cm indoors, covering the electric heating wires with disinfected sawdust, connecting an automatic temperature control instrument, inserting a temperature sensing head of the temperature control instrument in the middle of the cutting bed, enabling the bottom of the temperature sensing head to be parallel to the base of the cutting slips, and flattening the bed surface to form the cutting bed;
then, during cutting, firstly taking out a bundle of cutting slips, dipping the bottom of each bundle of cutting slips in a rooting solution for 5 hours, wherein the dipping depth is 4cm, the rooting solution is a composite rooting solution of 75ppm NAA and 150ppm IBA, then turning the bottom of each cutting slip downwards in wet saw dust left and right to enable the wet saw dust to be completely filled in gaps at the bottom of each cutting slip, and then placing the bundle of cutting slips in a cutting bed side by side, wherein the wet saw dust is filled in the bundle of cutting slips;
then, after cutting, the temperature of the electric heating hotbed is controlled at 25 ℃, the relative humidity is kept at 76%, water should be supplemented in time when the humidity is insufficient, 700 times of carbendazim liquid medicine is sprayed once a week after root forcing for one week, after half a month of cutting, a large amount of callus tissues are formed at the base parts of the cutting slips, a large amount of root systems are generated, and the cutting slips with the callus tissues obtained are transplanted into a nutrition cup for cultivating seedlings.
As can be seen from the comparison between the first embodiment and the second embodiment, the compound rooting solution used in the second embodiment requires dipping the cutting shoot in the rooting solution for a long time to achieve the corresponding effect, while in the first embodiment, the cutting shoot is dipped in the ABT1 rooting powder for several seconds at a medium speed to achieve the corresponding effect.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. A seabuckthorn hardwood cutting root-forcing method is characterized by comprising the following steps:
s1, constructing an indoor slotting machine: laying an electric heating wire indoors, covering the electric heating wire with disinfected sawdust, connecting an automatic temperature control instrument, inserting a temperature sensing head of the temperature control instrument in the middle of the cutting bed, enabling the bottom of the temperature sensing head to be parallel to the base of the cutting slips, and flattening the bed surface to form the cutting bed;
s2, collection of cutting shoots: collecting cuttings which are strong and do not break and have the diameter of more than or equal to 0.5cm and grow for 1-2 years from the sea buckthorn trees 2-3 weeks before the sea buckthorn sprouts in winter or spring;
s3, cutting: cutting the cuttings to obtain cuttings, bundling every 30-50 cuttings into a bundle, placing the bundle in a cellar, and covering and storing the bundle with wet sand;
s4, cutting and root forcing: taking out the bundled cutting slips, quickly dipping the bottoms of the bundled cutting slips for 10s by using ABT1 rooting powder, taking the upper parts of the processed cutting slips, enabling the bottoms of the cutting slips to face downwards, twisting the cutting slips left and right in wet saw dust to enable the wet saw dust to completely fill gaps at the bottoms of the cutting slips, then vertically arranging the bundled cutting slips in a cutting bed, filling saw dust between the cutting slips to reach the thickness of 2/3 with the length of cutting slips, and reserving 2-3 buds at the tops of the cutting slips;
s5, post-insertion processing: after the cutting is processed, the constant temperature and humidity of the seedbed are controlled, water is timely supplemented when the humidity is insufficient, 500 times of carbendazim liquid medicine is sprayed once a week after root forcing is carried out for 7-8 days, after cutting is carried out for 10-15 days, a large number of callus tissues are formed at the base part of the cutting, a large number of root systems are generated, and the cutting obtained the callus tissues is transplanted to a nutrition cup or a field for cultivating seedlings.
2. The method for inducing the roots of sea-buckthorn hardwood cutting according to claim 1, wherein the distance between adjacent electric heating wires in S1 is 10cm, the electric heating wires are parallel to each other, and the thickness of sawdust is 8cm-10 cm.
3. The method for inducing the root of the seabuckthorn by hardwood cutting as claimed in claim 1, wherein the method for sterilizing the sawdust in S1 comprises the following steps: spraying 0.2-0.3% potassium permanganate solution onto sawdust 1-2 days before cuttage, with a spraying amount of 3g/m2And fully leaching the sawdust for 3-4 hours by using clean water 24 hours after spraying.
4. The method for inducing the rooting of the hardwood cuttage of seabuckthorn according to claim 1, wherein when the cuttings in S3 are cut, the upper ends of the cuttings are cut, the lower ends of the cuttings are cut, and the moisture content of the sand in S3 is not less than 75%.
5. The method for inducing the roots of sea buckthorn through hardwood cutting according to claim 1, wherein the length of cuttings in S3 is 8-12 cm, and the diameter of cuttings is more than or equal to 0.5 cm.
6. The method for inducing the roots of seabuckthorn through hardwood cutting according to claim 1, wherein the preparation process of ABT1 rooting powder in S4 comprises the following steps: 1g of rooting powder is taken, dissolved by 95 percent of alcohol and then is added with distilled water to reach the constant volume of 1L.
7. The method for inducing the roots of the sea-buckthorn hardwood cutting as claimed in claim 1, wherein the temperature of the seedbed in the S5 is controlled to be 23-27 ℃, and the relative humidity is controlled to be 70-85%.
8. The method for inducing the roots of seabuckthorn through hardwood cutting as claimed in claim 1, wherein the callus formation rate at the base of the scion in S5 is greater than or equal to 85%.
CN202010546075.5A 2020-06-16 2020-06-16 Method for cutting and root-forcing of sea-buckthorn hardwood Pending CN111527898A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101889512A (en) * 2010-07-09 2010-11-24 新疆农垦科学院 Cutting seedling culture method of seabuckthorn tender branches in severe cold area and special seedling culture substrate
CN104081966A (en) * 2014-06-18 2014-10-08 安徽华箔箔业科技有限公司 Method for sea-buckthorn hard branch cutting seedling
JP2015027262A (en) * 2013-07-30 2015-02-12 住友林業株式会社 Method for raising cutting seedlings of forest trees
CN108738770A (en) * 2018-05-10 2018-11-06 单金友 A kind of Hard Branch Cottage Of Seabuckthorn method for culturing seedlings
CN110521414A (en) * 2019-09-24 2019-12-03 苏州大学 A kind of zelkova schneideriana electric seed bed heater hardwood cuttage method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101889512A (en) * 2010-07-09 2010-11-24 新疆农垦科学院 Cutting seedling culture method of seabuckthorn tender branches in severe cold area and special seedling culture substrate
JP2015027262A (en) * 2013-07-30 2015-02-12 住友林業株式会社 Method for raising cutting seedlings of forest trees
CN104081966A (en) * 2014-06-18 2014-10-08 安徽华箔箔业科技有限公司 Method for sea-buckthorn hard branch cutting seedling
CN108738770A (en) * 2018-05-10 2018-11-06 单金友 A kind of Hard Branch Cottage Of Seabuckthorn method for culturing seedlings
CN110521414A (en) * 2019-09-24 2019-12-03 苏州大学 A kind of zelkova schneideriana electric seed bed heater hardwood cuttage method

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Title
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