CN107318623A - A kind of method of Begonia masoniana cutting propagation - Google Patents
A kind of method of Begonia masoniana cutting propagation Download PDFInfo
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- CN107318623A CN107318623A CN201710662071.1A CN201710662071A CN107318623A CN 107318623 A CN107318623 A CN 107318623A CN 201710662071 A CN201710662071 A CN 201710662071A CN 107318623 A CN107318623 A CN 107318623A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
Abstract
The present invention relates to plant cuttage technical field, a kind of method of Begonia masoniana cutting propagation is disclosed, horizontal and vertical cutting is carried out along the vein of Begonia masoniana blade, obtained blade is as cuttings;Blade concentration after segmentation is soaked into 1~2h for 0.02~0.5g/L exogenous hormone, the blade after immersion is laid on the deep positions of 0.3~0.5cm of matrix;Air humidity is kept more than 95%, temperature control is at 22~26 DEG C, and water content of substrate is 80%~95%, is transplanted when root growth completely grows new plant.The present invention method it is simple, efficient, easy to operate, and blade tiling cuttage mode prevented and treated Begonia masoniana leaf insert reproductive process in there is rotten situation, so as to improve the breeding potential of Begonia masoniana.
Description
Technical field
The present invention relates to plant cuttage technical field, more particularly to a kind of method of Begonia masoniana cutting propagation.
Background technology
Begonia masoniana (Begonia masoniana), alias:Horseshoe Malus spectabilis, Begoniaceae, Begonia.Originate in
In China and Malaysia.Warm and moist is liked, winter humidity must not be less than 10 DEG C.Summer requires nice and cool, half cloudy and air humidity
Big environment, temperature is advisable with 22~25 DEG C, non-refractory, more than 32 DEG C, then slow-growing, is afraid of strong light direct beam.Air humidity
80% or so.Happiness is loose, draining is good, the loam rich in humus.Flower class is seen usually as lawn and ground, can be made middle or small
Type is potted plant, also can hanging basket plantation, suspension room in, or make scape case plantation.Shade plant.
Begonia masoniana is generally inserted method using plant division or leaf and bred.Division propagation enters behavior when changing basin with spring
It is good, select the fresh and tender root fragrant plant for having terminal bud to cut, per potted plant 2~3 sections.Leaf, which is inserted, to adopt mature leaf in 5~June, stay petiole
1cm is cut, and blade is cut into diameter 6~7cm sizes, inserts sand bed, can be potted plant after 2 months.Method is inserted to iron using existing leaf
Cross begonia is bred, and reproductive efficiency is low, and the problem of blade rots easily occurs.
The content of the invention
In view of this, the invention provides a kind of method of Begonia masoniana cutting propagation, iron cross autumn sea is solved
Chinese bush cherry leaf inserts perishable problem, improves the reproductive efficiency of Begonia masoniana cutting propagation.
In order to solve the above-mentioned technical problem, technical scheme is as follows:
The invention provides a kind of method of Begonia masoniana cutting propagation, along the vein of Begonia masoniana blade
Horizontal and vertical cutting is carried out, obtained blade is as cuttings;It is the outer of 0.02~0.5g/L by the blade concentration after segmentation
Exogenous estrogen soaks 1~2h, and the blade after immersion is laid on the deep positions of 0.3~0.5cm of matrix;Air humidity is kept to exist
More than 95%, temperature control is at 22~26 DEG C, and water content of substrate is 80%~95%, when root growth completely grows new plant
Transplanting;
Wherein, the exogenous hormone includes the heteroauxin and indolebutyric acid that equal proportion is mixed, or the Yin that equal proportion is mixed
Diindyl butyric acid and methyl α-naphthyl acetate.
It is preferred that, the matrix is 4:1 weight than mixing perlite and vermiculite, or 4:Perlite of 1 weight than mixing
And yellow soil, or river sand, expand the optional scope of matrix of Begonia masoniana cutting propagation.
It is preferred that, the matrix is placed on cutting bed, before cuttage with 0.5~1% KMnO4Solution disappears to cutting bed
Poison, is carried out disinfection with 0.3~0.5% carbendazim to matrix;After cuttage 1~2 week, every 3~7d sprays mass concentration into matrix
For 0.3~0.5% carbendazim.
It is preferred that, depth of the matrix in cutting bed is 40~60cm.
It is preferred that, the high plastic sheeting sheds of 50~100cm are also built in the matrix overhead, to control the temperature of cuttage
And humidity.
Prior art is compared, and the present invention has advantages below:The present invention directly utilizes the partial blade of Begonia masoniana,
Cutting propagation is carried out on the position deep using 0.3~0.5cm of matrix is laid in, by the control to exogenous hormone, suitable
The stockless blade breeding of Begonia masoniana is realized under cuttage environment.The method of the present invention is simple, efficient, easy to operate.Iron ten
Word begonia rotting rate in conventional leaf inserts mode reproductive process is high, and the present invention is by using the cuttage side being laid in matrix
Method, improves the breeding potential of Begonia masoniana.It is right due to the partial blade that the cuttage blade of the present invention is Begonia masoniana
Whether petiole no requirement (NR) is had in propagating materials, therefore the blade of same Begonia masoniana can be as multiple cuttage materials, greatly
The big utilization rate for improving propagating materials.
Embodiment
The invention provides a kind of method of Begonia masoniana cutting propagation, insert perishable to solve Begonia masoniana leaf
Rotten, breeding potential and the low technical problem of planting percent.
The present invention carries out cutting propagation using Begonia masoniana blade as cuttings.In the present invention, to Begonia masoniana
The processing mode of blade is:By Begonia masoniana blade along vein is crosscutting and rip cutting, cuttings is used as using the blade after segmentation.
Unrestricted to the leaf blade size and shape after segmentation, such way can increase Begonia masoniana propagating materials, greatly improve
The utilization rate of propagating materials.
In order to improve rooting efficiency of the Begonia masoniana in leaf inserts reproductive process, the present invention is preferably by the iron after segmentation
Cross begonia blade is soaked with exogenous hormone.In the present invention, the soaking concentration of exogenous hormone is 0.02~0.5g/L, is entered
One step is preferably 0.1~0.4g/L.Soak time of the cuttings in exogenous hormone in the present invention is 0.5~2h, preferably 1~
1.5h。
In the present invention, exogenous hormone is preferably following medicament:Using mass ratio as 1:The heteroauxin (IAA) and Yin of 1 mixing
Diindyl butyric acid (IBA).The exogenous hormone of the present invention can also be using mass ratio as 1:The indolebutyric acid (IBA) and methyl α-naphthyl acetate of 1 mixing
(α-NAA).The present invention is not particularly limited to the source of the exogenous hormone, using conventional city well known to those skilled in the art
Sell commodity.
Blade after exogenous hormone is soaked is laid in progress culture breeding in matrix.
It is preferably following matrix that Begonia masoniana leaf of the present invention, which inserts breeding,:With 4:Perlite of 1 weight than mixing and leech
Stone, or 4:1 weight is than the perlite and yellow soil of mixing, or river sand.The present invention is not particularly limited to the source of the matrix,
Using the matrix species of purchase.
The blade of the Begonia masoniana of the present invention is before the matrix middle period inserts, and preferred substrate is first disinfected.This hair
It is bright that the mode that matrix is disinfected is not particularly limited, using the conventional substrate disinfection way of this area.In the present invention
In specific embodiment, preferably cutting medium is carried out disinfection with carbendazim, the diluted concentration of carbendazim is preferably 0.3~0.5%,
More preferably 0.4%.Uniformly be sprayed on cutting medium in the morning, starts cuttage after preferably spraying 1d, to avoid the disease in matrix
Bacterium causes to rot to blade.
The cutting medium of the present invention, which is preferably disposed on cutting bed, carries out cuttage.The cutting bed needs to be disappeared before cuttage
Poison, preferably with 0.5~1% KMnO4Solution carries out spray disinfectant to bed surface.Disinfection way is preferably that sprinkling once, connects per 1d
Continuous sterilization 7d.After being carried out disinfection to cutting bed, the cutting medium after sterilization is placed in cutting bed.Preferred substrate is in cutting bed
Depth be 40~60cm, more preferably 45~56cm, meet growing state of the Begonia masoniana in matrix.
After matrix treatments are good, the leaf for carrying out Begonia masoniana inserts breeding.Blade after exogenous hormone is soaked is laid in
0.3~0.5cm of matrix depths, is preferably laid in 0.35~0.45cm of matrix depths.Temperature, humidity and illumination etc. are controlled during cuttage
Weather conditions, to increase the survival rate of cuttage.In the present invention, preferably cuttage when relative air humidity more than 95%, temperature
For 22~26 DEG C, preferably 23~25 DEG C.In order to preferably control the temperature and humidity of cuttage, preferably in the matrix of cutting bed
Sky builds plastic sheeting shed.The present invention is not particularly limited to the form and dimension of shed, can be according to cutting bed and cuttage
Point sets suitable shed.In the specific embodiment of the invention, the highly preferred of shed is 50~100cm, more preferably 70~
80cm。
Can test material take root, except the foreign genetic element depending on material itself there is provided the environmental condition taken root also extremely
Close important.Control temperature to be no more than 26 DEG C after cuttage, in good time water spray, keep air humidity more than 95%, the water content of matrix
Between 80%~95%, preferably 85%~90%.After cuttage 1~2 week, in good time spraying fungicide prevents cuttage blade rotten
It is rotten.The preferably every 3~7d of the present invention sprays the carbendazim of 1 time 0.3~0.5%.
Root growth is complete after 35d, is transplanted when new plant is grown.
The method of the present invention is simple, efficient, easy to operate, and Begonia masoniana rotting rate in leaf inserts reproductive process is low,
So as to improve the breeding potential of Begonia masoniana.
For the object, technical solutions and advantages of the present invention are more clearly understood, the present invention is entered with reference to embodiment
Row detailed description, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
Cutting medium selects 4:1 weight is than the perlite and vermiculite of mixing, and exogenous hormone used is the Yin that equal proportion is mixed
Indolylbutyric acid (IAA) and indolebutyric acid (IBA), the indolebutyric acid (IBA) and methyl α-naphthyl acetate (α-NAA) of equal proportion mixing.
Blade Cutting test is carried out in forest-science academy of Guizhou Province greenhouse.Cutting bed bottom spreads 50cm matrix respectively.For preferably
Temperature, humidity are controlled, plastic sheeting shed, high about 70cm are built in cuttage bed substrate overhead.Control relative humidity 95% in canopy
More than, 22~23 DEG C of temperature.
Material picks up from the maternal blade of adult respectively, with maternal leaf production test material.Each test method obtains 100
The blade of decile is not tested.Insert before first with 0.5% KMnO4Solution carries out disinfection to bed surface, then with 0.3% carbendazim
Cutting medium is carried out disinfection, it is 80~95% to control water supply in media during cuttage.By the Begonia masoniana blade edge of collection
That vein is crosscutting with into some not deciles of rip cutting, blade is soaked using the exogenous hormone (0.02~0.5mg/L) of various concentrations, when
Between be 1~2h.Blade is laid in respectively on the deep positions of matrix 0.5cm.After 3d situation of taking root is checked every 7d.
After cuttage 2 weeks, the carbendazim of 1 time 0.3% is sprayed weekly.According to arid situation, water spray number of times is determined, with containing for matrix
Water is degree in 80~95% or so (feel not water outlet is tack-free), keeps relative humidity more than 95% in canopy, temperature is no more than
26℃。
Embodiment 2
Cutting medium selects 4:1 weight is than the perlite and yellow soil of mixing, and exogenous hormone used is what equal proportion was mixed
Heteroauxin (IAA) and indolebutyric acid (IBA), the indolebutyric acid (IBA) and methyl α-naphthyl acetate (α-NAA) of equal proportion mixing.
Blade Cutting test is carried out in forest-science academy of Guizhou Province greenhouse.Cutting bed bottom spreads 40cm matrix respectively.For preferably
Temperature, humidity are controlled, plastic sheeting shed, high about 90cm are built in cuttage bed substrate overhead.Control relative humidity 95% in canopy
More than, 24~26 DEG C of temperature.
Material picks up from the maternal blade of adult respectively, with maternal leaf production test material.Each test method obtains 100
The blade of decile is not tested.Insert before first with 0.3% KMnO4Solution carries out disinfection to bed surface, then with 0.5% carbendazim
Cutting medium is carried out disinfection, it is 80~95% to control water supply in media during cuttage.By the Begonia masoniana blade edge of collection
That vein is crosscutting with into some not deciles of rip cutting, blade is soaked using the exogenous hormone (0.02~0.5mg/L) of various concentrations, when
Between be 1~2h.Blade is laid in respectively on the deep positions of matrix 0.3cm.After 3d situation of taking root is checked every 7d.
After cuttage 1 week, the carbendazim of spray 1 time 0.5% in every 3 days.According to arid situation, water spray number of times is determined, with matrix
Water content is degree in 80~95% or so (feel not water outlet is tack-free), keeps relative humidity more than 95% in canopy, temperature does not surpass
Cross 26 DEG C.
Embodiment 3
Cutting medium selects river sand, and exogenous hormone used is the heteroauxin (IAA) and indolebutyric acid that equal proportion is mixed
(IBA), the indolebutyric acid (IBA) and methyl α-naphthyl acetate (α-NAA) of equal proportion mixing.
Blade Cutting test is carried out in forest-science academy of Guizhou Province greenhouse.Cutting bed bottom spreads 60cm matrix respectively.For preferably
Temperature, humidity are controlled, plastic sheeting shed, high about 50cm are built in cuttage bed substrate overhead.Control relative humidity 95% in canopy
More than, 22~26 DEG C of temperature.
Material picks up from the maternal blade of adult respectively, with maternal leaf production test material.Each test method obtains 100
The blade of decile is not tested.Insert before first with 0.4% KMnO4Solution carries out disinfection to bed surface, then with 0.4% carbendazim
Cutting medium is carried out disinfection, it is 80~95% to control water supply in media during cuttage.By the Begonia masoniana blade edge of collection
That vein is crosscutting with into some not deciles of rip cutting, blade is soaked using the exogenous hormone (0.02~0.5mg/L) of various concentrations, when
Between be 1~2h.Blade is laid in respectively on the deep positions of matrix 0.4cm.After 3d situation of taking root is checked every 7d.
After cuttage 10 days, the carbendazim for 1 time 0.4% being sprayed into matrix in every 5 days.According to arid situation, water spray number of times is determined,
Relative humidity more than 95% in canopy is kept to spend in 80~95% or so (feel not water outlet is tack-free) with the water content of matrix,
Temperature is no more than 26 DEG C.
Comparative example
Exogenous hormone in embodiment 1~3 is replaced with into root-inducing powder (ABT), remaining method is constant.
Embodiment 4
Different substrates influence on the rooting efficiency of Begonia masoniana
As can be seen from Table 1:After cuttage 20d, each processing is most of to be started to take root, and respectively processing is most of big by 24d after inserting
Amount is taken root, and root system has grown completely after 35d, and each processing rooting rate is up to more than 98%.In addition, 3 kinds of matrix used are to iron ten
Word begonia test material rooting efficiency is:Perlite+vermiculite (4:1)>Perlite+yellow soil (4:1)>River sand, planting percent is equal
Reach more than 95%.
The different substrates cuttage root-taking situation of table 1
Embodiment 5
Seedling influential effect of the hormon to Begonia masoniana
Result of the test shows:3 kinds of matrix used are perlite+vermiculite to Begonia masoniana test material seedling result
(4:1)>Perlite+yellow soil (4:1)>River sand, wherein perlite+vermiculite (4:1) (hormone concentration 0.1-0.4g/L), perlite
+ yellow soil (4:1) (hormone concentration 0.1-0.4g/L) planting percent reaches more than 80%;Heteroauxin (IAA)+indolebutyric acid
(IBA)(1:1) hormone combinations, indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations are better than root-inducing powder (ABT)
Number of seedling.
The different exogenous hormone processing seedling effect situations of table 2
Embodiment 6
Different exogenous hormones taking root in different substrates, seedling situation
As can be seen from Table 3, matrix cuttage Begonia masoniana, heteroauxin (IAA)+Yin are used as using perlite and vermiculite
Diindyl butyric acid (IBA) (1:1) hormone combinations, indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations are 0.1 in concentration
During~0.4g/L, its rooting rate is more than 95%, and planting percent is more than 98%.And heteroauxin (IAA)+indolebutyric acid (IBA)
(1:1) hormone combinations effect is better than indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations.
Table 3 IAA+IBA, IBA+ α-NAA processing is in the taking root of perlite and vermiculite matrix, seedling situation
As can be seen from Table 4, using perlite and yellow soil as matrix cuttage Begonia masoniana, heteroauxin (IAA)+
Indolebutyric acid (IBA) (1:1) hormone combinations, indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations are in concentration
During 0.1~0.4g/L, its rooting rate is more than 92%, and planting percent is more than 96%.Heteroauxin (IAA)+indolebutyric acid (IBA)
(1:1) hormone combinations effect is better than indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations.
Table 4 IAA+IBA, IBA+ α-NAA processing is in the taking root of perlite and yellow soil matrix, seedling situation
As can be seen from Table 5, using river sand as matrix cuttage Begonia masoniana, heteroauxin (IAA)+indolebutyric acid
(IBA)(1:1) hormone combinations, indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations are 0.1~0.4g/ in concentration
During L, its rooting rate is more than 90%, and planting percent is more than 94%.Heteroauxin (IAA)+indolebutyric acid (IBA) (1:1) hormone
Combined effect is better than indolebutyric acid (IBA)+methyl α-naphthyl acetate (α-NAA) (1:1) hormone combinations.
IAA+IBA the and IBA+ α-NAA of table 5 processing is in the taking root of river sand matrix, seedling situation
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (5)
1. a kind of method of Begonia masoniana cutting propagation, it is characterised in that the vein along Begonia masoniana blade enters
The horizontal and vertical cutting of row, obtained blade is as cuttings;By the external source that the blade concentration after segmentation is 0.02~0.5g/L
Hormone soaks 1~2h, and the blade after immersion is laid on the deep positions of 0.3~0.5cm of matrix;Air humidity is kept 95%
More than, temperature is 22~26 DEG C, and water content of substrate is 80%~95%, is transplanted when root growth completely grows new plant;
Wherein, the exogenous hormone includes the heteroauxin and indolebutyric acid that equal proportion is mixed, or the indoles fourth that equal proportion is mixed
Acid and methyl α-naphthyl acetate.
2. according to the method described in claim 1, it is characterised in that the matrix is river sand, or 4:Pearl of 1 weight than mixing
Rock and vermiculite, or 4:Perlite and yellow soil of 1 weight than mixing.
3. method according to claim 1 or 2, it is characterised in that the matrix is placed on cutting bed, with 0.5 before cuttage
~1% KMnO4Solution is carried out disinfection to cutting bed, and matrix is carried out disinfection with 0.3~0.5% carbendazim;Cuttage 1~2
Zhou Hou, every 3~7d spray the carbendazim that mass concentration is 0.3~0.5% into matrix.
4. method according to claim 3, it is characterised in that depth of the matrix in cutting bed is 40~60cm.
5. method according to claim 4, it is characterised in that also build the high modelings of 50~100cm in the matrix overhead
Expect film shed.
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CN108935101A (en) * | 2018-08-13 | 2018-12-07 | 云南省农业科学院花卉研究所 | A method of reducing lathe work begonia leaf tissue culture melting brown rate |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108668657A (en) * | 2018-04-19 | 2018-10-19 | 北京林业大学 | The stem of distant hybridization Cultivars of Chinese Herbaceous Peony inserts propagation method between a kind of group |
CN108935101A (en) * | 2018-08-13 | 2018-12-07 | 云南省农业科学院花卉研究所 | A method of reducing lathe work begonia leaf tissue culture melting brown rate |
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Application publication date: 20171107 |