CN105145371A - Tissue culture breeding method for cymbaria dahurica - Google Patents
Tissue culture breeding method for cymbaria dahurica Download PDFInfo
- Publication number
- CN105145371A CN105145371A CN201510658610.5A CN201510658610A CN105145371A CN 105145371 A CN105145371 A CN 105145371A CN 201510658610 A CN201510658610 A CN 201510658610A CN 105145371 A CN105145371 A CN 105145371A
- Authority
- CN
- China
- Prior art keywords
- explant
- medium
- wuli
- fragrant plant
- tube plantlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 16
- 241000155172 Cymbaria Species 0.000 title abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 13
- 239000002609 medium Substances 0.000 claims abstract description 13
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 24
- 238000005520 cutting process Methods 0.000 claims description 18
- 238000000338 in vitro Methods 0.000 claims description 18
- 239000005972 6-Benzyladenine Substances 0.000 claims description 12
- 229920001817 Agar Polymers 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 12
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 12
- 239000006870 ms-medium Substances 0.000 claims description 12
- 230000001902 propagating effect Effects 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 239000003599 detergent Substances 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 230000006698 induction Effects 0.000 claims description 6
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 6
- 229960001669 kinetin Drugs 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229960002523 mercuric chloride Drugs 0.000 claims description 6
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 6
- 235000020679 tap water Nutrition 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000004161 plant tissue culture Methods 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 27
- 230000035755 proliferation Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000003444 anaesthetic effect Effects 0.000 description 3
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 208000034507 Haematemesis Diseases 0.000 description 1
- 206010021531 Impetigo Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 230000003356 anti-rheumatic effect Effects 0.000 description 1
- 239000003435 antirheumatic agent Substances 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Abstract
A tissue culture breeding method for cymbaria dahurica comprises the following steps: (1) taking a cymbaria dahurica seed as an explant, and disinfecting the explant; (2) placing the disinfected explant in an MS minimal medium, and inducing the explant, so as to obtain a sterile test-tube plantlet; (3) placing the sterile test-tube plantlet in an MS breeding culture medium, and carrying out test-tube plantlet rapid breeding culture, so as to obtain cluster buds. According to the invention, the growth coefficient of the cymbaria dahurica cluster buds obtained by adopting the method can reach 15-25 times, and cymbaria dahurica high-grade seedlings with high survival rate can be provided in a short period, so that the problem about the large-scale seedling culture of cymbaria dahurica is effectively solved.
Description
Technical field
The present invention relates to a kind of method for propagation, particularly a kind of Da Wuli core fragrant plant tissue culture method for breeding.
Background technology
Da Wuli core fragrant plant CymbariadahuricaL., for Scrophulariaceae core fragrant plant platymiscium, also Cymbaria dahurica, Herba Cymbariae Dahuricae is claimed, anaesthetic name " I Tan-A gives ", it is one of Original plant of Mongolian medicine's medicine dedicated core fragrant plant, be born on desert steppe between height above sea level 620-110 rice and upland meadow more, be mainly distributed in the ground such as the Inner Mongol, Heilungkiang, Hebei.All herbal medicine, has dry " Xieri Wusu Symptom ", heat-clearing, the effects such as wines used as antirheumatic, diuresis, hemostasis, be used for the treatment of rheumathritis, haematemesis, bleeding from five sense organs or subcutaneous tissue, have blood in stool, traumatism and bleeding, nephritic dropsy, the disease such as impetigo.
At present, the core fragrant plant medicinal material on market is mainly from wild resource, and along with the raising of its medical value, market increases the demand of medicinal material, and cause Da Wuli core fragrant plant to be excavated by transition, wild resource reserves also reduce increasingly, and resource is on the verge of exhaustion.Although the concern of recent domestic to anaesthetic constantly increases, anaesthetic core fragrant plant also result in the attention of many scholars, and research report constantly increases, and mainly concentrates on resource distribution, Qualitive test, chemical composition and pharmacological research.Up to the present, report is had no for the artificial planting of core fragrant plant, the correlative study of seedling breeding technology.Fragrant plant is generally bred by seed Da Wuli core, but find in preliminary seedling breeding work, the seminal propagation of Da Wuli core fragrant plant exists that germination rate is low, irregular, the problem such as seedling quality is unstable of germinateing, and seriously constrains standardized planting and the production of Da Wuli core fragrant plant.Adopt biotechnology tissue culture technique, reproduction speed and the quality of Da Wuli core fragrant plant seedling can be improved effectively rapidly, realize the factorial seedling growth of Da Wuli core fragrant plant high quality seedling, with the needs in satisfied production.
Summary of the invention
The object of this invention is to provide a kind of Da Wuli core fragrant plant tissue culture method for breeding, it can Fast-propagation go out a large amount of be applicable to transplanting excellent Da Wuli core fragrant plant seedling, meet need of production.
The present invention achieves the above object by the following technical programs: a kind of Da Wuli core fragrant plant tissue culture method for breeding, is characterized in that: the method comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, with the addition of 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant;
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 0.1-1.0mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.1-0.3mg/L of 0.5-2.5mg/L in MS propagating culture medium, the pH value of medium is 5.8.
Outstanding advantages of the present invention is:
(1) biotechnology is adopted to carry out tissue-culturing quick-propagation to Da Wuli core fragrant plant; by the breeding of Da Wuli core fragrant plant Multiple Buds; a large amount of Da Wuli core fragrant plant seedling being applicable to cultivating and growing can be cultivated at short notice; ensure growth coefficient and the seedling quality of Da Wuli core fragrant plant seedling; accomplish scale production, meet the needs on producing.
(2) the Da Wuli core fragrant plant adventitious buds proliferation coefficient adopting the present invention to obtain reaches 15-25 doubly, and Multiple Buds is healthy and strong, easily takes root after being inoculated into root media.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further illustrated.
Embodiment 1
An example of the quick breeding method for tissue culture of Da Wuli core fragrant plant of the present invention, comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, by explant successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, be added into 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant;
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 0.1mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.1mg/L of 0.5mg/L in MS propagating culture medium, the pH value of medium is 5.8, growth multiplying power is 7.0 times, and adventitious buds proliferation coefficient is 15.5.
Embodiment 2
Another example of the quick breeding method for tissue culture of Da Wuli core fragrant plant of the present invention, comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, by explant successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, be added into 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant;
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 0.1mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.3mg/L of 1.5mg/L in MS propagating culture medium, the pH value of medium is 5.8, growth multiplying power is 18.1 times, and adventitious buds proliferation coefficient is 20.5.
Embodiment 3
Another example of the quick breeding method for tissue culture of Da Wuli core fragrant plant of the present invention, comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, by explant successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, be added into 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant;
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 0.5mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.3mg/L of 1.0mg/L in MS propagating culture medium, the pH value of medium is 5.8, growth multiplying power is 15.8 times, and adventitious buds proliferation coefficient is 19.2.
Embodiment 4
Another example of the quick breeding method for tissue culture of Da Wuli core fragrant plant of the present invention, comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, by explant successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, be added into 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant;
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 1500lux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 1.0mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.3mg/L of 2.5mg/L in MS propagating culture medium, the pH value of medium is 5.8, growth multiplying power is 20.3 times, and adventitious buds proliferation coefficient is 24.5.
Claims (1)
1. Yi Zhong Da Wuli core fragrant plant tissue culture method for breeding, is characterized in that: the method comprises the following steps:
(1) selection of explant and sterilization: get the full fruit of Da Wuli core fragrant plant, seed is taken out as explant after peelling off pericarp, successively with 2v/v% liquid detergent aqueous solution soaking 5min, wire tap water 15-30min, with the addition of 2-3 and drip 100 milliliters of 0.1v/v% mercuric chloride sterilization 8-10min of Tween-20, aseptic water washing 3-5 time, finally remove surface moisture with sterilized filter paper, obtain aseptic explant
(2) explant just generation induction obtains in vitro cuttings: be inoculated in MS medium by the aseptic explant that step (1) obtains, be 23-27 DEG C in cultivation temperature, intensity of illumination 15001ux, light application time is cultivate under the condition of 8-10 hour/day to obtain in vitro cuttings in 45 days, wherein add heteroauxin IAA, 30g/L sucrose of 6-benzyladenine 6-BA, 0.1mg/L and the agar of 3.4g/L of 1.0mg/L in MS medium, the pH value of medium is 5.8;
(3) test-tube plantlet Multiple Buds Fast-propagation is cultivated: the in vitro cuttings obtained in step (2) is placed in MS propagating culture medium, at cultivation temperature 23-27 DEG C, intensity of illumination 15001ux, light application time is cultivate under the condition of 8-10 hour/day to obtain test-tube plantlet Multiple Buds in 75 days, wherein add kinetin KT, 30g/L sucrose of heteroauxin IAA, 0.1-1.0mg/L and the agar of 3.4g/L of 6-benzyladenine 6-BA, 0.1-0.3mg/L of 0.5-2.5mg/L in MS propagating culture medium, the pH value of medium is 5.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510658610.5A CN105145371B (en) | 2015-10-12 | 2015-10-12 | A kind of Da Wuli cores fragrant plant tissue culture method for breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510658610.5A CN105145371B (en) | 2015-10-12 | 2015-10-12 | A kind of Da Wuli cores fragrant plant tissue culture method for breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105145371A true CN105145371A (en) | 2015-12-16 |
| CN105145371B CN105145371B (en) | 2017-06-23 |
Family
ID=54786773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510658610.5A Active CN105145371B (en) | 2015-10-12 | 2015-10-12 | A kind of Da Wuli cores fragrant plant tissue culture method for breeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105145371B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365483A (en) * | 2014-11-20 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Scrophularia ningpoensis hemsl breeding method |
| CN104472062A (en) * | 2014-12-31 | 2015-04-01 | 包头医学院 | Method for promoting cymbariae seeds to germinate |
-
2015
- 2015-10-12 CN CN201510658610.5A patent/CN105145371B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365483A (en) * | 2014-11-20 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Scrophularia ningpoensis hemsl breeding method |
| CN104472062A (en) * | 2014-12-31 | 2015-04-01 | 包头医学院 | Method for promoting cymbariae seeds to germinate |
Non-Patent Citations (2)
| Title |
|---|
| JING-QIU DAI等: ""Non-glycosidic iridoids from Cymbaria mongolica"", 《PHYTOCHEMISTRY》 * |
| 熊英: ""蒲包花种子组织培养的研究"", 《安徽农业科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105145371B (en) | 2017-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN104335903A (en) | Method for accelerating quick propagation of rhizoma bletillae | |
| CN104082138B (en) | A kind of tissue cultivation rapid breeding method of Radix Aristolochiae Tagalae | |
| CN101889547B (en) | Aseptic and rapid propagation method of dendrobium devonianum seeds | |
| CN105145359B (en) | Tissue culture and rapid propagation method for asparagus filicinus | |
| CN102550413A (en) | Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua | |
| CN107155898B (en) | A kind of dendrobium candidum carries out expanding numerous method using stem section thin slice | |
| CN103202229B (en) | Tissue culturing and rapid propagating method for chloranthy florida var. plena | |
| CN102144566A (en) | Method for culturing test tube plantlet of Xingren Anoectochilus | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN105028206A (en) | Tissue culture propagation method for Hainan dendrobe | |
| CN105191803A (en) | Dendrobium officinale tissue culture bag seedling production method | |
| CN103477976B (en) | A kind of Herba Dendrobii stem section tissue culture method | |
| CN102845306A (en) | Tissue culture breeding method of Acanthopanax senticosus | |
| CN105409773A (en) | Lophophora williamsii sterile seeding and regeneration system establishing method | |
| CN105724245A (en) | Micro-cutting method for rapid propagation of Aquilaria sinensis seedlings | |
| CN105265310A (en) | Method for breeding raspberry seedling through tissue culture | |
| CN104145825B (en) | The method of artichoke test tube seedling stem apex rapid seedling cultivation | |
| CN107211897A (en) | A kind of method that Bulbilus boussingaultiae pseudobaselloidis vitro Regeneration System is set up | |
| CN105104197A (en) | Method for culturing and breeding dendrobium guangxiense tissue | |
| CN105145371B (en) | A kind of Da Wuli cores fragrant plant tissue culture method for breeding | |
| CN105724246A (en) | Method for rapid propagation of floating fern seedling | |
| CN104770299B (en) | Method for sterilely germinating gentianella acuta seeds | |
| CN104737915B (en) | Rhododendron vialii tissue culture method taking leaves as explant | |
| CN105165623A (en) | Cymbaria mongolica maxim tissue culture breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20151216 Assignee: Tengxian Mingfeng Agricultural Technology Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980046049 Denomination of invention: A Method for Tissue Culture and Breeding of Dawuli Xinba Granted publication date: 20170623 License type: Common License Record date: 20231108 |
|
| EE01 | Entry into force of recordation of patent licensing contract |