CN103204746B - Special biological composite bacterium fertilizer for platycodon grandiflorum - Google Patents
Special biological composite bacterium fertilizer for platycodon grandiflorum Download PDFInfo
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- CN103204746B CN103204746B CN201310160072.8A CN201310160072A CN103204746B CN 103204746 B CN103204746 B CN 103204746B CN 201310160072 A CN201310160072 A CN 201310160072A CN 103204746 B CN103204746 B CN 103204746B
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Abstract
The invention relates to a biological composite bacterium fertilizer and belongs to the field of bio-fertilizer. A special biological composite bacterium fertilizer for platycodon grandiflorum is composed of the following components in percentage by weight: 5-10% of bactericide, 55-60% of macromolecule natural organic matter, 10-13% of large granular urea, 10-12% of controlled-release sulfur coated urea, and 5-15% of nitrogen-phosphorus-potassium compound fertilizer. The biological composite bacterium fertilizer provided by the invention has the advantages that the quantity and activity of the beneficial microorganism are improved, the harmful microorganism is inhibited, the physical and chemical properties of soil are improved, the nutrients of soil are increased and balanced, and the residues of farm chemicals and fertilizer and other harmful matters in the soil are degraded. After the biological composite bacterium fertilizer is applied, platycodon grandiflorum can be repeatedly planted in non continuous cropping or continuous cropping land, and the quality and the yield of platycodon grandiflorum are improved.
Description
Technical field
The present invention relates to microbial fertilizer field, particularly relate to a kind of balloonflower root special bio compound bacterial fertilizer.
Background technology
Balloonflower root (
platycodon grandifloruma.DC.) being the dry root of campanulaceae per nnial herb, is one of maximum 40 kinds of traditional Chinese medicine materials of China's sales volume, and property is flat, and bitter is pungent, has effect of preventing phlegm from forming and stopping coughing, relieving sore throat to recover voice, the smooth lung qi of a surname, removing pus and relieving carbuncle.Modern study shows, that balloonflower root also has is hypotensive, vasodilation, hypoglycemic, cholinolytic, antianaphylaxis, antitumor and improve the pharmacologically active widely such as immunizing power, thus be widely used in various prescriptions and clinical in.Balloonflower root China, Japan, Korea S do medicinal in, China, Korea and Korea S are also edible in a large number, and in American-European, Japan and Korea S also as gardening ornamental plant and cut-flower.
Balloonflower root is as medicine, food, reward dual-purpose plant, the long-term demand of China approximately 6 × 10
6t, and demand has cumulative year after year trend, depends merely on wild balloonflower root resource and can not satisfy the demands.The seventies in last century, China starts big area artificial culture balloonflower root, through the development of decades, has progressively formed Shandong Zibo, Taihe county, Anhui, San great planting base, Chifeng.But three large bases are all faced with a problem: root rot, blight, the root knot nematode disease that balloonflower root continuous cropping causes having a strong impact on output and the quality of three large base balloonflower roots.The medicinal herb grower in three large bases divides and is clipped to exotic species and plants medicinal material, causes three large balloonflower root planting base output sharply to decline.Meanwhile, the balloonflower root of non-main producing region output, mouthfeel is poor, drug effect is low, has affected the export volume of balloonflower root.
Medicinal herb grower plants planting platycodon radix mainly to execute inorganic fertilizer as main, and fertilising causes soil compaction, severe environmental pollution, utilization rate of fertilizer to reduce, and disease and pest increases, and causes planting cost increase, balloonflower root output and Quality Down, Benefit Drops.
At present, balloonflower root is mainly take base manure as main, and before full-bloom stage, imposing potash fertilizer and nitrogenous fertilizer is auxiliary fertilizing method simultaneously.The main drawback of the method is: the nitrogenous fertilizer in base manure, and easily cause balloonflower root overground part growth in seedling stage vigorous, root growth is slow, causes later stage balloonflower root lodging, yield reducation.Topdress and be beneficial to growing of balloonflower root later stage root.But, spreading fertilizer over the fields, utilization rate of fertilizer is lower; Row replacement, easily injures balloonflower root root and takes a lot of work.Therefore, the fertilizer practice of balloonflower root also needs to improve and upgrade.
Summary of the invention
The object of the invention is to, overcome the deficiencies in the prior art, a kind of balloonflower root specific complex bacterial manure is provided, can effectively improve balloonflower root field soil probiotics quantity, reduce balloonflower root soil-borne disease; Bulk application, does not need to topdress, and can single-dose application meet the required nutrition of the each growth period of balloonflower root; According to grow regulation of fertilizer requirement fertilising of balloonflower root, avoid soil pollution, improvement Soil structure because overnutrition causes; Finally, reduce and produce balloonflower root cost, improve kind of a planting platycodon radix benefit.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of balloonflower root special bio compound bacterial fertilizer, its composition and weight percent content are: microbial inoculum accounts for 5 ~ 10%, macromole natural organic matter accounts for 55 ~ 60%, granular urea 10 ~ 13%, release-controlled coated urea 10 ~ 12%, phosphorus potassium complex fertilizer 5 ~ 15%, described microbial inoculum is made up of bacterial classification and solid medium, microbial inoculum and solid medium weight ratio 1:20, wherein the bacterial classification in microbial inoculum is yellow green trichoderma W2256.
The yellow green trichoderma of described bacterial classification (
trichoderma aureoviride) W2256 separation and Culture and mutagenesis gained from Trichoderma, this bacterial strain is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences; Preserving number: CGMCC No.
6494.Preservation date: on September 3rd, 2012.
Described macromole natural organic matter refers to that dregs of beans mixes according to weight 1:1 with Pericarppium arachidis hypogaeae, corn cob or stalk one wherein.
Described phosphorus potassium complex fertilizer is made up of diammonium phosphate and potassium sulfate, wherein P
2o
5and K
2the weight ratio of O is 8:4.According to balloonflower root regulation of fertilizer requirement, adopt (0-8-4) phosphorus potassium complex fertilizer, wherein diammonium phosphate, potash fertilizer potassium sulfate for phosphate fertilizer.
Described solid medium is made up of wheat bran, acid-sludge and water, and its weight ratio is 2:1:1.33.
0.2 hundred million/gram of viable count content in described microbial inoculum.
Balloonflower root biological composite bacterial manure optimum ratio by weight percentage: microbial inoculum accounts for 8%, macromole natural inorganic organism accounts for 57%, and granular urea accounts for 12%, and release-controlled coated urea accounts for 13%, phosphorus potassium complex fertilizer 9%.
Granular urea, release-controlled coated urea etc. are by being sold in market.
Yellow green trichoderma (
trichoderma aureoviride) W2256 separates mutagenic processes:
Separation method: the starting strain of this bacterium picks up from Jinan forest land of mountain area soil, uses PDA substratum+thousandth Streptomycin sulphate to carry out Isolation and Culture, then carries out monospore separation, obtains purebred yellow green trichoderma.Continue enlarged culturing and obtain after a large amount of conidiums, make spore spore content about 10 alive
7individual/ml suspension, puts in test tube to be mutagenic.
Mutafacient system: apply this institute
60co-r x ray irradiation x device carries out mutagenesis to above bacteria suspension, and dosage range is selected 5-10kGry.According to it, indexes such as cucumber fusarium axysporum bacteriostasis rate size, sporulation quantity are screened for bacterial strain after mutagenesis, finally obtain yellow green trichoderma W2256
the preparation of microbial inoculum
1) cultivation of bacterial classification
By 4 ℃ of mould W2256 strain transfers of wood to potato dextrose agar flat board (PDA) central authorities, 26 ℃ of cultivations.By the mould spore of cultured wood on PDA flat board, access and be equipped with in the 250ml triangular flask of 100ml potato glucose (PD) nutrient solution with sterilized water dilution, 26 ℃, 180r/min, shaking culture 3 days, as bacterial classification.
2) enlarged culturing
Seed liquor 1.0ml is added in the liquid nutrient medium (Semen Maydis powder 15g/L, bean powder 5g/L add suitable quantity of water to boil rear 30min, get juice by four layers of filtered through gauze, are diluted with water to 1000ml) that Semen Maydis powder and bean powder make to 26 ℃ of shaking culture.
3) fixing cultivation
Cultured trichoderma strain W2256 liquid is moved into and fills 100g wheat bran with 5% ratio: in the 500ml triangular flask of the solid medium of acid-sludge: water=2:1:1.33,26 ℃ of cultivations.
4) bacterium inspection is dried, and pulverizes.
The present invention, by adding yellow green trichoderma W2256 bacterial classification, can effectively control the soil-borne diseases such as balloonflower root root rot, blight, root knot nematode disease, overcomes the problem that balloonflower root can not continuous cropping; According to balloonflower root nutritional requirement, macroelement is carried out to rational proportion, meet the balloonflower root desired nutritional that grows; Application slowly-releasing fertilizer practice, supplies with fertilizer according to balloonflower root regulation of fertilizer requirement, reduces fertilizer loss, improves balloonflower root Quality and yield; By adding the organic macromolecule such as dregs of beans and Pericarppium arachidis hypogaeae material, improve Soil structure, for growing, balloonflower root provides superior edatope.
Yellow green trichoderma W2256 microbial inoculum: effectively control balloonflower root root rot, blight, root knot nematode disease, yellow green trichoderma W2256 has the antagonistic action of wide spectrum to phytopathogen, test shows that root rot, blight, Pathogen of Root-knot Disease bacterium effect to balloonflower root are better.Meanwhile, can also produce multiple enzyme and decompose the plant and animal residues in soil, produce the organism that plant can be absorbed.
Granular urea, release-controlled coated urea: granular urea can meet the amount of the required nitrogenous fertilizer of balloonflower root early growth, avoids growth nitrogenous fertilizer in early stage excessive, and the cauline leaf excessive growth causing, causes the plant lodging in later stage, affects kind and the output of balloonflower root; Also nitrogenous fertilizer for want of not, makes plant strain growth slow, reduces plant height, reduces output.Release-controlled coated urea, mainly meets the required nitrogenous fertilizer of balloonflower root the middle and late growth stage.
Phosphorus potassium complex fertilizer: adopt phosphorus potassium complex fertilizer, supplement the under-nutrition that single use urea causes, suitable proportioning can improve balloonflower root yield and quality.
The invention has the beneficial effects as follows: balloonflower root biological composite bacterial manure, by biological prevention, has overcome because of root rot, blight, root knot nematode disease that continuous cropping causes, improve land utilization ratio and yield rate; Balloonflower root biological composite bacterial manure is in guaranteeing fertilizer abundance, guarantee that again it efficiently comprehensively absorbs, the enzyme decomposition plant and animal residues that release-controlled coated urea control degradation of urea and yellow green trichoderma W2256 utilization produce becomes absorbable nutritive substance and carries out simultaneously.Slow released urea improves Soil Micro-environment, is beneficial to the mould growth of wood; Add and wood is mould the decomposition of accelerating larger molecular organics, and microenvironment after decomposing is more conducive to wooden mould growth, soil is improved, be beneficial to the absorption of its nutrition after urea decomposition, complement each other between the two, there is no antagonistic action, make its industrialization become possibility.Wheat bran and acid-sludge provide nutrient for wood is mould, and are beneficial to the growth of bacterial classification.
Balloonflower root biological composite bacterial manure is an organic whole, and each effect is mutually promoted, and improves the microenvironment of soil from many aspects; and balloonflower root biological composite bacterial manure is according to balloonflower root regulation of fertilizer requirement; the rational application of fertilizers providing, can not cause secondary pollution, is conducive to environment protection.The Trichoderma group who selects, except having phytopathogen antagonistic action, can also provide the function of various active thing and various organized enzymes comprehensively.
Embodiment
embodiment 1
Balloonflower root biological composite bacterial manure configuration proportion by weight percentage: microbial inoculum accounts for 9%, macromole natural organic matter accounts for 57%, granular urea 12%, release-controlled coated urea 12%, phosphorus potassium complex fertilizer 10%.Wherein macromole natural organic matter is mixed according to weight 1:1 with Pericarppium arachidis hypogaeae by dregs of beans.Mix pack.
The impact of table 1 balloonflower root biological composite bacterial manure on disease and pest and quality and yield
| Fertilizer type | Root rot infection rate (%) | Root knot nematode disease infection rate (%) | Soluble sugar (%) | Protein (%) | Output (kg/667m2) | Volume increase (%) |
| Common compound fertilizer | 36.52 | 49.83 | 38.37 | 3.46 | 631.6 | ? |
| Balloonflower root compound bacterial fertilizer | 0.18 | 0.22 | 45.77 | 4.96 | 997.8 | 57.98 |
Table 1 data declaration, balloonflower root biological composite bacterial manure can significantly suppress the root knot nematode disease and the root rot that cause because of continuous cropping compared with Common compound fertilizer; And improve soluble protein and sugar matter content; Amount of increase in production reaches 57.98% simultaneously.
embodiment 2
Balloonflower root biological composite bacterial manure configuration proportion by weight percentage:
Microbial inoculum accounts for 10%, macromole natural organic matter 55%, granular urea 12%, release-controlled coated urea 12%, phosphorus potassium complex fertilizer 11%.Wherein macromole natural organic matter is mixed according to weight 1:1 with corn cob by dregs of beans.Mix pack.
The impact of table 2 balloonflower root biological composite bacterial manure on disease and output
Table 2 Data support, balloonflower root biological composite bacterial manure can significantly suppress the root knot nematode disease and the root rot that cause because of continuous cropping; And improve its output.
embodiment 3
Balloonflower root biological composite bacterial manure configuration proportion by weight percentage:
Microbial inoculum accounts for 5%, macromole natural organic matter 60%, granular urea 11%, release-controlled coated urea 11%, phosphorus potassium complex fertilizer 13%.Wherein macromole natural organic matter is mixed according to weight 1:1 with stalk by dregs of beans.Mix pack.
The impact of table 3 balloonflower root biological composite bacterial manure on disease and quality and yield
Table 3 data declaration, balloonflower root biological composite bacterial manure can not only reduce root knot nematode disease and root rot incidence, improve output, meanwhile, improves soluble protein and sugar matter, increases mouthfeel.
embodiment 4
Balloonflower root biological composite bacterial manure configuration proportion by weight percentage:
Microbial inoculum accounts for 8%, macromole natural organic matter 58%, granular urea 12%, release-controlled coated urea 12%, phosphorus potassium complex fertilizer 10%.Wherein macromole natural organic matter is mixed according to weight 1:1 with Pericarppium arachidis hypogaeae by dregs of beans.Mix pack.
The impact of table 4 balloonflower root biological composite bacterial manure on disease and output and surviving rate
Table 4 data declaration, balloonflower root biological composite bacterial manure has reduced the root knot nematode disease and the root rot sickness rate that cause because of continuous cropping compared with Common compound fertilizer; Improve balloonflower root output; Strengthen balloonflower root keeping crop seedling from drought ability.(damage caused by a drought is more serious then, and after plantation, 2 wheat harvesting periods just have effective precipitation.Keeping crop seedling from drought is windfall.）
Claims (1)
1. a balloonflower root special bio compound bacterial fertilizer, it is characterized in that: its composition and weight percent content are: microbial inoculum accounts for 5 ~ 10%, macromole natural organic matter accounts for 55 ~ 60%, granular urea 10 ~ 13%, release-controlled coated urea 10 ~ 12%, phosphorus potassium complex fertilizer 5 ~ 15%, described microbial inoculum is made up of bacterial classification and solid medium, bacterial classification and solid medium weight ratio 1:20, wherein the bacterial classification in microbial inoculum is yellow green trichoderma W2256; Described macromole natural organic matter refers to that dregs of beans mixes according to weight 1:1 with Pericarppium arachidis hypogaeae, corn cob or stalk one wherein; The yellow green trichoderma of described bacterial classification (
trichoderma aureoviride) W2256 separation and Culture and mutagenesis gained from Trichoderma, this bacterial strain is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, and preserving number is CGMCC No.
6494.
2. a kind of balloonflower root special bio compound bacterial fertilizer according to claim 1, is characterized in that: described phosphorus potassium complex fertilizer is made up of diammonium phosphate and potassium sulfate, wherein P
2o
5and K
2the weight ratio of O is 8:4.
3. a kind of balloonflower root special bio compound bacterial fertilizer according to claim 1, is characterized in that: described solid medium is made up of wheat bran, acid-sludge and water, its weight ratio is 2:1:1.33.
4. a kind of balloonflower root special bio compound bacterial fertilizer according to claim 1, is characterized in that: 0.2 hundred million/gram of viable count content in described microbial inoculum.
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| CN201310160072.8A CN103204746B (en) | 2012-11-08 | 2013-05-03 | Special biological composite bacterium fertilizer for platycodon grandiflorum |
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| CN201210443172.7 | 2012-11-08 | ||
| CN201210443172 | 2012-11-08 | ||
| CN201310160072.8A CN103204746B (en) | 2012-11-08 | 2013-05-03 | Special biological composite bacterium fertilizer for platycodon grandiflorum |
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| CN103204746B true CN103204746B (en) | 2014-05-21 |
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| CN103467185B (en) * | 2013-08-23 | 2014-12-10 | 河南省农业科学院植物保护研究所 | Biological compound organic bacterial fertilizer |
| CN103936516B (en) * | 2014-04-25 | 2016-08-24 | 陈华 | Formula and the preparation method of bio-feritlizer is prepared with leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. |
| CN106747721B (en) * | 2017-02-28 | 2020-08-18 | 四川省天府神龙中药饮片有限公司 | Special compound microbial fertilizer for platycodon grandiflorum and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5071462A (en) * | 1985-12-27 | 1991-12-10 | Yosiaki Kimura | Method and apparatus for producing organic fertilizer with the use of nitrogen fixing bacillus |
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101638328A (en) * | 2008-10-14 | 2010-02-03 | 中国农业科学院农业资源与农业区划研究所 | Straw organic fertilizer and preparation method thereof |
| CN102503703A (en) * | 2011-11-22 | 2012-06-20 | 刘长福 | Novel compound fertilizer containing active microorganisms |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020103083A1 (en) * | 2000-08-11 | 2002-08-01 | Harman Gary E. | Promoting deeper root development, reducing nitrogen fertilizer usage, imparting drought resistance, and increasing tolerance to adverse soil conditions in plants |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5071462A (en) * | 1985-12-27 | 1991-12-10 | Yosiaki Kimura | Method and apparatus for producing organic fertilizer with the use of nitrogen fixing bacillus |
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101638328A (en) * | 2008-10-14 | 2010-02-03 | 中国农业科学院农业资源与农业区划研究所 | Straw organic fertilizer and preparation method thereof |
| CN102503703A (en) * | 2011-11-22 | 2012-06-20 | 刘长福 | Novel compound fertilizer containing active microorganisms |
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