CN109497099A - A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight - Google Patents
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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Abstract
The invention discloses the environmentally protective novel biopesticides that one kind can effectively prevent Asparagus Stem Blight, belong to Strategies of Agricultural Bio-control technical field.The novel biopesticide is made of the raw material of following parts by weight: 10-20 parts of chitosan oligosaccharide, 20-40 parts of Chinese herbal medicine activity extract, 10-40 parts of carbendazim, 8-15 parts of surfactant, 100-200 parts of water.The present invention is by the synergistic effect between chitosan oligosaccharide, carbendazim and Chinese herbal medicine activity extract, the effect of capable of playing significant prevention and treatment Asparagus Stem Blight disease, promote asparagus production capacity；Novel biopesticide safety, green, no pollution to the environment are conducive to the sustainable development of planting asparagus industry.
Description
Technical field
The present invention relates to a kind of novel biopesticide methods of efficiently prevention and treatment Asparagus Stem Blight, more particularly, to a kind of synthesis
Using the biological pesticide of the efficient prevention and treatment Asparagus Stem Blight of chitosan oligosaccharide, Chinese herbal medicine extract and carbendazim preparation, belong to agriculture life
Object Prevention Technique field.
Background technique
Asparagus (scientific name: Asparagusofficinalis) also known as asparagus are Asparagaceae Asparagus (genus asparagus)
Herbaceos perennial, tender seedling have the laudatory title of " kings of vegetables " for cooked food in the international market.Asparagus nutritive value is high, energy
Moistening lung, antibechic, eliminating the phlegm, and have the function of to inhibit tumour growth etc., it is very popular.In recent years, with asparagus cultivation face
Product expands, and disease generation aggravates year by year, and the generation and harm of especially stem wilt have seriously affected the output and quality of asparagus.
The pathogen of Asparagus Stem Blight is lucid asparagus Phomopsis, is a kind of universal destructive disease；The disease
Evil it is rainy have wind under conditions of infect rapidly, rainwater, which splashes, to be stained with and can also infect.The main reason for infection through air is large area morbidity
The direction of field sprawling and morbidity are often affected by wind rapidly.In addition, hypsography low-lying, soil property is glutinous heavy, and nitrogenous fertilizer is excessive etc., easily add
The onste again.The disease occurs in the Asia planting asparagus country such as China, Japan, Thailand, Indonesia than more serious, especially with China
The most serious, China asparagus production province occurs generally, and south overweights the north.Less serious case leads to asparagus retarded growth,
Reduce yield and quality；Severe one diseased plant is withered in advance, and full field is destroyed.
Currently, the prevention and treatment of Asparagus Stem Blight is mainly controlled by chemical pesticides, but due to largely using chemical agent for many years
Prevention and treatment causes the germ stronger drug resistance occur in many areas and the effect of pesticide is made to have a greatly reduced quality.Lin Peili etc. and health
Industry is refined to have carried out different pesticides to the toxicity test of asparagus stem wilt bacteria synergy；Peng Mingsheng and Feng Xiao case determines 60% salt
Sour carbendazol wettable powder, 70% thiophanate-methyl wettable powder, 50% benomyl wettable powder, 50% difoltan can
The preventive effect of the medicaments such as wet powder and 12.5% olefin conversion wettable powder, but above-mentioned medicament is service life longer sterilization
Agent, asparagus stem wilt bacteria are also easy to produce drug resistance to these fungicide, therefore the surrogate-data technique for finding prevention and treatment stem wilt is particularly important.
Patented technology 2017105568113 discloses a kind of novel life of sterilization for preventing and treating Asparagus Stem Blight, asparagus splash disease
Object pesticide, main component are hexamethylene, zineb and thiophanate-methyl；It can be seen that the patented technology still with
Based on chemical prevention；Patented technology 2013103015752 discloses a kind of chemical fertilizer and its fertilizing method for preventing and treating Asparagus Stem Blight,
Its key technology is to adjust the nitrogen phosphoris and potassium fertilizer ratio of chemical fertilizer, keeps soil conservation acid, destroy the onset condition of stem wilt, belong to
In " temporary solution ".In conclusion based on chemical agent, novel biological agent is main for the prevention and treatment of Asparagus Stem Blight in the prior art
For preventing the morbidity of Asparagus Stem Blight, and it is relatively fewer to the preventive effect for the Asparagus Stem Blight fallen ill, to be easy to cause agriculture
Residual excess influences food safety, reduces asparagus productivity effect.
Summary of the invention
For the defect for making up the prior art, the present invention provides a kind of new bio agricultures of efficiently prevention and treatment Asparagus Stem Blight
Medicine can significantly play prevention and treatment asparagus by the synergistic effect between the raw materials such as chitosan oligosaccharide, Chinese herbal medicine activity extract and carbendazim
Stem wilt improves Yield of Asparagus and quality, to improve planting asparagus benefit；Novel biopesticide safety, green, to environment
It is pollution-free, conducive to the sustainable development of planting asparagus industry.
To achieve the above object, The technical solution adopted by the invention is as follows:
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is prepared from the following raw materials in parts by weight: chitosan oligosaccharide
10-20 parts, 20-40 parts of Chinese herbal medicine activity extract, 10-40 parts of carbendazim, 8-15 parts of surfactant, 100-200 parts of water.
Preferably, the novel biopesticide of a kind of efficiently prevention and treatment Asparagus Stem Blight as described above, by following weight fraction
Raw material be made: 12-16 parts of chitosan oligosaccharide, 25-32 parts of Chinese herbal medicine activity extract, 20-30 parts of carbendazim, surfactant 8-15
Part, 100-200 parts of water.
It is furthermore preferred that a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight as described above, by following weight point
Several raw materials are made: 15 parts of chitosan oligosaccharide, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, 10 parts of surfactant, water 150
Part.
The molecular weight of above-mentioned chitosan oligosaccharide is 1000-2000Da, wherein especially best with the chitosan oligosaccharide of 1500Da molecular weight.
Above-mentioned carbendazim is 50% wettable powder.
Said herbal medicine activity extract is the water extract of the root of purple-flowered peucedanum, cordate houttuynia, Radix Glycyrrhizae, wherein the compatibility of herbal raw material
Ratio is 25 parts of the root of purple-flowered peucedanum, 18 parts of cordate houttuynia, 7 parts of Radix Glycyrrhizae.
Said herbal medicine activity extract is prepared via a method which: deimpurity herbal raw material will be gone to crush, cross 100
5 times of herbal raw material of pure water is added in mesh, and 5% cellulase of herbal raw material mass ratio, 3% zytase is added,
50-60 DEG C of enzymatic hydrolysis 12h of temperature control under the conditions of ultrasonic wave booster action；It is filtered after enzymatic hydrolysis, filtrate is that Chinese herbal medicine activity is extracted
Object.
The present invention also provides a kind of preparation method of the novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight as described above,
The following steps are included:
1) chitosan oligosaccharide, carbendazim, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), carbendazim, surfactant are put into the water of formula ratio, is stirred to complete
Dissolution；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, obtains efficiently
The novel biopesticide for preventing and treating Asparagus Stem Blight.
In the present invention, chitosan oligosaccharide due to relative molecular mass it is small, be easy to through bacterial membrane easily enter cytoplasm and
In nucleus, the relevant enzyme for making its cell interior play physiological activity is revealed, while mutual with negatively charged substance such as DNA
Effect, interferes the duplication and transcription of DNA, to play the effect of its antimicrobial acivity；Chitosan oligosaccharide also can induce the anti-of plant
Characteristic of disease generates immune and killing effect to a variety of fungies, bacterium and virus.Chinese herbal medicine activity extract plays in the present invention
Important facilitation is obviously improved the suppression of product of the present invention by functional activities substances such as saponins in Chinese herbal medicine active constituent
The effect of bacterium sterilization, controlling disease；And it is a discovery of the invention that chitosan oligosaccharide, carbendazim and Chinese herbal medicine activity extract are used in combination
With stronger slow-release function, extend the action time of product of the present invention.
Carbendazim is a kind of broad-spectrum germicide, to various crop disease as caused by fungi (such as Fungi Imperfecti, more sac fungus)
Evil has control efficiency, can be used for foliar spray, seed treatment and soil treatment etc..Existing result of study shows carbendazim to reed
The control efficiency of bamboo shoot stem wilt is poor, and excessive application can produce drug resistance.The present invention is living by compounding chitosan oligosaccharide, Chinese herbal medicine
Property extract and carbendazim after discovery carbendazim can quickly inhibit the morbidity of Asparagus Stem Blight under the auxiliary of above-mentioned raw materials,
Stem wilt is reduced to asparagus production bring harm.
The present invention further provides novel biopesticide the answering in planting asparagus of above-mentioned efficient prevention and treatment Asparagus Stem Blight
With method, it is characterised in that it is described it is efficient prevention and treatment Asparagus Stem Blight novel biopesticide be diluted with water to 200-300 times of liquid into
Row is spraying, and usage amount is in 500-1000g/hm2。
Compared with the prior art, the invention has the following beneficial effects:
(1) present invention is withered by the synergistic effect raising prevention and treatment asparagus stem of chitosan oligosaccharide, carbendazim, Chinese herbal medicine activity extract
Effect of disease, makes product have effects that significantly to prevent Asparagus Stem Blight, changes and prevent Asparagus Stem Blight in the prior art
With chemical microbial inoculum or change the technology drawback of bitter bamboo shoot growth conditions, it is residual to reduce planting asparagus process Chinese medicine, have a wide range of application, and
Have effects that significantly to increase production, is remarkably improved the food safety and planting benefit of asparagus；
(2) product of the present invention is environmentally protective, and the primary raw material of selection is chitosan oligosaccharide and Chinese herbal medicine active constituent, to agricultural life
Long secondary environmental pollution is smaller；
(3) carbendazim of quick sterilization and the chitosan oligosaccharide of long-acting bacteriostatic and Chinese herbal medicine active constituent are combined by the present invention
It uses, can not only play the effect of quickly inhibiting stem wilt, but also the morbidity again of stem wilt can be inhibited in a long time；
(4) present invention mainly contains chitosan oligosaccharide, carbendazim and Chinese herbal medicine activity extract, these three substance collective effects,
With significant synergistic function, makes novel biopesticide of the present invention in prevention and treatment Asparagus Stem Blight, improve Yield of Asparagus and product
Matter significant effect, and achieve the technical effect of " 1+1 > 2 "；
(5) the raw material group of Chinese herbal medicine activity extract of the present invention becomes the root of purple-flowered peucedanum, cordate houttuynia, Radix Glycyrrhizae, and wherein the root of purple-flowered peucedanum evacuates wind
Heat, cordate houttuynia is clearing heat and detoxicating, Radix Glycyrrhizae coordinating the drug actions of a prescription；The experimental results showed that single Chinese herbal medicine has no significant prevention Asparagus Stem Blight
Effect, the effect of prevention and treatment stem wilt of certain effect can be played after the root of purple-flowered peucedanum, cordate houttuynia and the reasonable compatibility of Radix Glycyrrhizae, more
It is important that the effect of product of the present invention prevention and treatment Asparagus Stem Blight can be remarkably promoted.
Specific embodiment
In the examples below, Chinese herbal medicine activity extract is prepared by following technique: will remove deimpurity Chinese herbal medicine
Raw material crushes, and sieves with 100 mesh sieve, the pure water of 5 times of herbal raw material of addition, addition 5% cellulase of herbal raw material mass ratio,
3% zytase, 50-60 DEG C of enzymatic hydrolysis 12h of temperature control under the conditions of ultrasonic wave booster action；It is filtered after enzymatic hydrolysis, filtrate is
Chinese herbal medicine activity extract.
In following embodiments, the molecular weight of chitosan oligosaccharide is in 1500Da or so；The above-mentioned carbendazim of carbendazim is 50% wettable
Property pulvis.
Embodiment 1
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
15 parts, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, 10 parts of surfactant, 150 parts of water；
A kind of preparation method of the novel biopesticide of above-mentioned efficiently prevention and treatment Asparagus Stem Blight, comprising the following steps:
1) chitosan oligosaccharide, carbendazim, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), carbendazim, surfactant are put into the water of formula ratio, is stirred to complete
Dissolution；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, obtains efficiently
The novel biopesticide for preventing and treating Asparagus Stem Blight；
The novel biopesticide of above-mentioned efficient prevention and treatment Asparagus Stem Blight is diluted with water to 300 times of liquid and is sprayed, usage amount
In 600g/hm2。
Embodiment 2
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
16 parts, 32 parts of Chinese herbal medicine activity extract, 30 parts of carbendazim, 15 parts of surfactant, 200 parts of water；
Preparation method and application method is the same as embodiment 1.
Embodiment 3
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
12 parts, 25 parts of Chinese herbal medicine activity extract, 20 parts of carbendazim, 8 parts of surfactant, 100 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 1 (contain only Chinese herbal medicine activity extract, the other the same as in Example 1) in novel biopesticide
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: Chinese herbal medicine of the raw material of following weight fraction
70 parts of activity extract, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 2 (contain only chitosan oligosaccharide, the other the same as in Example 1) in novel biopesticide
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
70 parts, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 3 (contain only carbendazim, the other the same as in Example 1) in novel biopesticide
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: carbendazim of the raw material of following weight fraction
70 parts, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1；
Comparative examples 4 (are free of Chinese herbal medicine activity extract, the other the same as in Example 1) in novel biopesticide
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
15 parts, 25 parts of carbendazim, 10 parts of surfactant, 180 parts of water；
Preparation method and application method is the same as embodiment 1；
Comparative examples 5 (herbal raw material contains only the root of purple-flowered peucedanum, the other the same as in Example 1 in novel biopesticide)
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
15 parts, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1；
Comparative examples 6 (herbal raw material contains only cordate houttuynia, the other the same as in Example 1 in novel biopesticide)
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
15 parts, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1；
Comparative examples 7 (herbal raw material contains only Radix Glycyrrhizae, the other the same as in Example 1 in novel biopesticide)
A kind of novel biopesticide of efficient prevention and treatment Asparagus Stem Blight, is made: chitosan oligosaccharide of the raw material of following weight fraction
15 parts, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, 10 parts of surfactant, 150 parts of water；
Preparation method and application method is the same as embodiment 1.
Influence of the more different novel biopesticides of embodiment 4 to Asparagus Stem Blight control efficiency and target for increased production
1, disease survey
Every cell is using 5 points of diagonal line samplings, and every takes connected 5 clumps of asparagus, and 3 asparagus of every clump of asparagus random searching are total
25 clumps of asparagus, 75 asparagus are investigated, healthy tree number and diseased plant number at different levels are recorded.Mark of the severity Scaling standard referring to formulations such as Yang Yingqing
It is quasi-.0 grade: without morbidity；1 grade: onset area accounts for 6% or less the gross area；2 grades: onset area accounts for the 6%~10% of the gross area；3
Grade: onset area accounts for the 11%~20% of the gross area；4 grades: onset area accounts for the 21%~30% of the gross area；5 grades: onset area
Account for 30% or more of the gross area；
2, test method
The experiment recorded in same Yang Yingqing, Lan Bo etc. " control efficiency of 6 kinds of fungicide to Asparagus Stem Blight " text in test
Method；
3, data processing calculation method
Disease index=∑ (diseased plant numbers at different levels × opposite value of series)/investigation total strain number × 5 × 100；
Control efficiency=(check plot disease index-treatment region disease index)/check plot disease index × 100%；
Rate of growth=(treatment region yield-blank district yield)/blank district yield × 100%；
4, experimental result
The different novel biopesticides of table 1 are prevented and treated stem wilt and the influence of target for increased production
| Testing index | Preventive effect (%) | Rate of growth (%) |
| 1 test group of embodiment | 85.57±1.32 | 17.26±1.04 |
| 2 test group of embodiment | 82.29±1.44 | 15.63±1.22 |
| 3 test group of embodiment | 83.05±1.59 | 16.97±1.06 |
| 1 group of comparative examples | 27.45±1.09 | 11.45±0.94 |
| 2 groups of comparative examples | 25.37±1.38 | 9.57±0.87 |
| 3 groups of comparative examples | 19.91±1.26 | 8.74±1.15 |
| 4 groups of comparative examples | 48.36±1.16 | 12.92±1.44 |
| 5 groups of comparative examples | 55.29±1.41 | 13.08±1.23 |
| 6 groups of comparative examples | 58.80±1.13 | 13.18±1.09 |
| 7 groups of comparative examples | 52.17±0.95 | 12.86±1.27 |
As shown in table 1, test result shows that compared with control group routine operation, embodiment 1-3 prevents Asparagus Stem Blight
Effect illustrates that product of the present invention has significant control efficiency to Asparagus Stem Blight 82% or more.During comparative examples 1 contain only
Herbal medicine activity extract is 27.46% to Asparagus Stem Blight preventive effect maximum value, illustrates Chinese herbal medicine activity extract in spite of one
Determine prophylactic-therapeutic effect, but effect is not significant；Comparative examples 2 contain only chitosan oligosaccharide, are free of Chinese herbal medicine activity extract and carbendazim,
It is 25.37% to stem wilt preventive effect, but difference is more significant compared with embodiment 1-3；Comparative examples 3 contain only carbendazim, no
Activity extract containing Chinese herbal medicine and chitosan oligosaccharide are 19.91% to stem wilt preventive effect, but difference is relatively aobvious compared with embodiment 1-3
It writes；Comparative example 1-3 is comprehensively compared to the control efficiency of Asparagus Stem Blight, illustrates that chitosan oligosaccharide, carbendazim and Chinese herbal medicine activity are extracted
There is significant synergistic function between object, the technical effect of " 1+1 > 2 " is achieved in the prevention and treatment to Asparagus Stem Blight.Together
When, experimental result also shows that product of the present invention has remarkable efficacy for promoting Yield of Asparagus, and changing products material composition can show
Write the growth promotion effect (P < 0.01) for reducing product.It is comprehensively compared, determines that embodiment 1 is preferred embodiment.
The influence for analyzing the pairs of biological pesticide asparagus disease control efficiency of herbal raw material group, passes through comparative examples 4-7 group
The experimental results showed that the composition for changing herbal raw material can significantly reduce biological pesticide to the control efficiency of Asparagus Stem Blight, use up
The pipe root of purple-flowered peucedanum, cordate houttuynia and Radix Glycyrrhizae to enhancing biological pesticide prophylactic-therapeutic effect have certain synergistic effect, but the root of purple-flowered peucedanum, cordate houttuynia and
Synergistic function between Radix Glycyrrhizae is the basic reason place of herbal raw material prevention and treatment Asparagus Stem Blight.
The above examples are only used to illustrate the technical scheme of the present invention, rather than is limited；Although referring to aforementioned reality
It applies example to be described in detail to by invention, but for those of ordinary skill in the art, it still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features；And to these modifications
Or replacement, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight, it is characterised in that by the raw material system of following parts by weight
At: 10-20 parts of chitosan oligosaccharide, 20-40 parts of Chinese herbal medicine activity extract, 10-40 parts of carbendazim, 8-15 parts of surfactant, water
100-200 parts.
2. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that by with
The raw material of lower weight fraction is made: 12-16 parts of chitosan oligosaccharide, 25-32 parts of Chinese herbal medicine activity extract, 20-30 parts of carbendazim, surface
8-15 parts of activating agent, 100-200 parts of water.
3. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that by with
The raw material of lower weight fraction is made: 15 parts of chitosan oligosaccharide, 30 parts of Chinese herbal medicine activity extract, 25 parts of carbendazim, surfactant 10
Part, 150 parts of water.
4. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that institute
The molecular weight for stating chitosan oligosaccharide is 1000-2000Da, wherein especially best with the chitosan oligosaccharide of 1500Da molecular weight.
5. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that institute
Stating carbendazim is 50% wettable powder.
6. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that institute
The water extract that Chinese herbal medicine activity extract is the root of purple-flowered peucedanum, cordate houttuynia, Radix Glycyrrhizae is stated, wherein the proportion compatibility of herbal raw material is the root of purple-flowered peucedanum
25 parts, 18 parts of cordate houttuynia, 7 parts of Radix Glycyrrhizae.
7. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 6, it is characterised in that described
Chinese herbal medicine activity extract is prepared via a method which: deimpurity herbal raw material will be gone to crush, sieved with 100 mesh sieve, in addition
5% cellulase of herbal raw material mass ratio, 3% zytase is added in the pure water that 5 times of herbal raw material, assists in ultrasonic wave
50-60 DEG C of enzymatic hydrolysis 12h of temperature control under action condition；It is filtered after enzymatic hydrolysis, filtrate is Chinese herbal medicine activity extract.
8. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that preparation
Technique the following steps are included:
1) chitosan oligosaccharide, carbendazim, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), carbendazim, surfactant are put into the water of formula ratio, is stirred to completely molten
Solution；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, is efficiently prevented and treated
The novel biopesticide of Asparagus Stem Blight.
9. a kind of novel biopesticide of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that described
Efficiently the novel biopesticide of prevention and treatment Asparagus Stem Blight is diluted with water to 200-300 times of liquid and is sprayed, and usage amount is in 500-
1000g/hm2。
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| CN110269065A (en) * | 2019-06-26 | 2019-09-24 | 江西省农业科学院植物保护研究所 | A kind of composition and its application method for preventing and treating Asparagus Stem Blight |
| CN111226965A (en) * | 2020-03-20 | 2020-06-05 | 江西省农业科学院植物保护研究所 | Composition for efficiently preventing and treating stem blight of asparagus, and preparation method and application thereof |
| CN114982787A (en) * | 2022-06-24 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating stem blight of asparagus and preparation method thereof |
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| CN110269065A (en) * | 2019-06-26 | 2019-09-24 | 江西省农业科学院植物保护研究所 | A kind of composition and its application method for preventing and treating Asparagus Stem Blight |
| CN111226965A (en) * | 2020-03-20 | 2020-06-05 | 江西省农业科学院植物保护研究所 | Composition for efficiently preventing and treating stem blight of asparagus, and preparation method and application thereof |
| CN114982787A (en) * | 2022-06-24 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating stem blight of asparagus and preparation method thereof |
| CN114982787B (en) * | 2022-06-24 | 2024-04-19 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating asparagus stem blight and preparation method thereof |
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