CN109452319A - It is a kind of it is efficient prevention and treatment Asparagus Stem Blight composition and its application - Google Patents
It is a kind of it is efficient prevention and treatment Asparagus Stem Blight composition and its application Download PDFInfo
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses the environmentally protective compositions that one kind can effectively prevent Asparagus Stem Blight, belong to Strategies of Agricultural Bio-control technical field.The composition is made of the raw material of following parts by weight: 10-20 parts of chitosan oligosaccharide, alga oligosaccharides 5-10 parts compound, 30-50 parts of Chinese herbal medicine activity extract, 8-15 parts of surfactant, 200-400 parts of water.The present invention is by the synergistic effect between chitosan oligosaccharide, compound alga oligosaccharides and Chinese herbal medicine activity extract, the effect of capable of playing significant prevention and treatment Asparagus Stem Blight disease, promote asparagus production capacity；The composition safety, green, no pollution to the environment are conducive to the sustainable development of planting asparagus industry.
Description
Technical field
The present invention relates to the compositions and its application method of a kind of efficiently prevention and treatment Asparagus Stem Blight, more particularly, to a kind of comprehensive
The composition for closing the efficient prevention and treatment Asparagus Stem Blight prepared using marine bioactivity oligosaccharides and Chinese herbal medicine extract, belongs to agricultural
Technical field of biological control.
Background technique
Asparagus (scientific name: Asparagus officinalis) also known as asparagus are Asparagaceae Asparagus (genus asparagus)
Herbaceos perennial, tender seedling have the laudatory title of " kings of vegetables " for cooked food in the international market.Asparagus nutritive value is high, energy
Moistening lung, antibechic, eliminating the phlegm, and have the function of to inhibit tumour growth etc., it is very popular.In recent years, with asparagus cultivation face
Product expands, and disease generation aggravates year by year, and the generation and harm of especially stem wilt have seriously affected the output and quality of asparagus.
The pathogen of Asparagus Stem Blight is lucid asparagus Phomopsis, is a kind of universal destructive disease；The disease
Evil it is rainy have wind under conditions of infect rapidly, rainwater, which splashes, to be stained with and can also infect.The main reason for infection through air is large area morbidity
The direction of field sprawling and morbidity are often affected by wind rapidly.In addition, hypsography low-lying, soil property is glutinous heavy, and nitrogenous fertilizer is excessive etc., easily add
The onste again.The disease occurs in the Asia planting asparagus country such as China, Japan, Thailand, Indonesia than more serious, especially with China
The most serious, China asparagus production province occurs generally, and south overweights the north.Less serious case leads to asparagus retarded growth,
Reduce yield and quality；Severe one diseased plant is withered in advance, and full field is destroyed.
Currently, the prevention and treatment of Asparagus Stem Blight is mainly controlled by chemical pesticides, but due to largely using chemical agent for many years
Prevention and treatment causes the germ stronger drug resistance occur in many areas and the effect of pesticide is made to have a greatly reduced quality.Lin Peili etc. and health
Industry is refined to have carried out different pesticides to the toxicity test of asparagus stem wilt bacteria synergy；Peng Mingsheng and Feng Xiao case determines 60% salt
Sour carbendazol wettable powder, 70% thiophanate-methyl wettable powder, 50% benomyl wettable powder, 50% difoltan can
The preventive effect of the medicaments such as wet powder and 12.5% olefin conversion wettable powder, but above-mentioned medicament is service life longer sterilization
Agent, asparagus stem wilt bacteria are also easy to produce drug resistance to these fungicide, therefore the surrogate-data technique for finding prevention and treatment stem wilt is particularly important.
Patented technology 2017105568113 discloses a kind of bactericidal composition for preventing and treating Asparagus Stem Blight, asparagus splash disease,
Its main component is hexamethylene, zineb and thiophanate-methyl；It can be seen that the patented technology is still anti-with chemistry
Based on controlling；Patented technology 2013103015752 discloses a kind of chemical fertilizer and its fertilizing method for preventing and treating Asparagus Stem Blight, crucial
Technology is to adjust the nitrogen phosphoris and potassium fertilizer ratio of chemical fertilizer, keeps soil conservation acid, destroy the onset condition of stem wilt, belong to and " control
Mark ".In conclusion in the prior art long-acting, non-environmental pollution control Asparagus Stem Blight composition and its application are not disclosed, it is right
The improvement of traditional stem wilt control method is still field technology drawback urgently to be resolved.
Summary of the invention
For the defect for making up the prior art, the present invention provides a kind of composition of efficiently prevention and treatment Asparagus Stem Blight and its answer
Prevention and treatment can significantly be played by the synergistic effect between chitosan oligosaccharide, compound alga oligosaccharides and Chinese herbal medicine activity extract with method
Asparagus Stem Blight improves Yield of Asparagus and quality, to improve planting asparagus benefit；The composition safety, green, to environment without
Pollution, conducive to the sustainable development of planting asparagus industry.
To achieve the above object, The technical solution adopted by the invention is as follows:
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, is prepared from the following raw materials in parts by weight: chitosan oligosaccharide 10-20
Part, alga oligosaccharides 5-10 parts compound, 30-50 parts of Chinese herbal medicine activity extract, 8-15 parts of surfactant, 200-400 parts of water.
Preferably, the composition of a kind of efficiently prevention and treatment Asparagus Stem Blight as described above, by the raw material of following weight fraction
It is made: 13-17 parts of chitosan oligosaccharide, alga oligosaccharides 6-8 parts compound, 38-45 parts of Chinese herbal medicine activity extract, surfactant 10-12
Part, 250-350 parts of water.
It is furthermore preferred that a kind of composition of efficiently prevention and treatment Asparagus Stem Blight as described above, by the original of following weight fraction
Material is made: 15 parts of chitosan oligosaccharide, 7 parts of compound alga oligosaccharides, 40 parts of Chinese herbal medicine activity extract, 11 parts of surfactant, water 300
Part.
The molecular weight of above-mentioned chitosan oligosaccharide is 1000-2000Da, wherein especially best with the chitosan oligosaccharide of 1500Da molecular weight.
Above-mentioned compound alga oligosaccharides refer to the mixture of brown alga oligose and carrageenan oligosaccharide, mass ratio 8:2.It is described
Alga oligosaccharides are advisable with digesting oligosaccharides.
Said herbal medicine activity extract is the water extract of the root of fangji, Artemisia japonica, purple perilla, wherein the compatibility ratio of herbal raw material
Example is 25 parts of the root of fangji, 18 parts of Artemisia japonica, 7 parts of purple perilla.
Said herbal medicine activity extract is prepared via a method which: deimpurity herbal raw material will be gone to crush, cross 100
20 times of herbal raw material of pure water is added in mesh, and 5% cellulase of herbal raw material mass ratio, 3% zytase is added,
50-60 DEG C of enzymatic hydrolysis 6h of temperature control under the conditions of ultrasonic wave booster action；It is filtered after enzymatic hydrolysis, filtrate is that Chinese herbal medicine activity is extracted
Object.
The present invention also provides a kind of preparation methods of the composition of efficiently prevention and treatment Asparagus Stem Blight as described above, including with
Lower step:
1) chitosan oligosaccharide, compound alga oligosaccharides, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), compound alga oligosaccharides, surfactant are put into the water of formula ratio, stirring
To being completely dissolved；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, obtains efficiently
The composition for preventing and treating Asparagus Stem Blight.
In the present invention, chitosan oligosaccharide due to relative molecular mass it is small, be easy to through bacterial membrane easily enter cytoplasm and
In nucleus, the relevant enzyme for making its cell interior play physiological activity is revealed, while mutual with negatively charged substance such as DNA
Effect, interferes the duplication and transcription of DNA, to play the effect of its antimicrobial acivity；Chitosan oligosaccharide also can induce the anti-of plant
Characteristic of disease generates immune and killing effect to a variety of fungies, bacterium and virus.Compound alga oligosaccharides promote crop to nutrient
It absorbs, promotes the growth of root system, increase yield, healthy and strong plant, enhance resistance, have the function that well established and vigorously developing, improving quality,
Improve bacteriostasis antibiosis effect of asparagus；Chinese herbal medicine activity extract plays important facilitation in the present invention, in
The functional activities substance such as saponin in herbal medicine active constituent is obviously improved the function of the restraining and sterilizing bacteria of product of the present invention, controlling disease
Effect；And it is a discovery of the invention that chitosan oligosaccharide, compound alga oligosaccharides and Chinese herbal medicine activity extract are used in combination with stronger sustained release
Function extends the action time of product of the present invention.
The present invention further provides application method of the composition in planting asparagus of above-mentioned efficient prevention and treatment Asparagus Stem Blight,
It is characterized in that the composition of the efficient prevention and treatment Asparagus Stem Blight is diluted with water to 20-30 times of liquid and carries out foliar spray.
Compared with the prior art, the invention has the following beneficial effects:
(1) present invention improves prevention and treatment reed by the synergistic effect of chitosan oligosaccharide, compound alga oligosaccharides, Chinese herbal medicine activity extract
The effect of bamboo shoot stem wilt, makes product have effects that significantly to prevent Asparagus Stem Blight, changes and prevent asparagus in the prior art
For stem wilt with chemical microbial inoculum or the drawbacks of change bitter bamboo shoot growth conditions, it is residual to reduce planting asparagus process Chinese medicine, has a wide range of application,
And have effects that significantly to increase production, it is remarkably improved the food safety and planting benefit of asparagus.The experimental results showed that the application group
It closes object and 88% or more is reached to the preventive effect of Asparagus Stem Blight；
(2) product of the present invention is environmentally protective, and the raw material of selection is plant extract product, to agriculture growing environment without any
Secondary pollution；
(3) product of the present invention uses filling liquid, and production cost is low, and production technology is conducive to large-scale promotion；
(4) present invention mainly contains chitosan oligosaccharide, compound alga oligosaccharides and Chinese herbal medicine activity extract, these three substances are common
Effect has significant synergistic function, makes the present composition in prevention and treatment Asparagus Stem Blight, improves Yield of Asparagus and quality
Significant effect, and achieve the technical effect of " 1+1 > 2 "；
(5) the raw material group of Chinese herbal medicine activity extract of the present invention become the root of fangji, Artemisia japonica, purple perilla, wherein root of fangji wind-expelling pain-stopping,
Clearing away heat and promoting diuresis, Artemisia japonica relieving superficies by cooling, purple perilla can dissipate exterior cold；The experimental results showed that single Chinese herbal medicine has no prevention Asparagus Stem Blight
The effect of, the effect of prevention and treatment stem wilt of certain effect can be played after the reasonable compatibility of the root of fangji, Artemisia japonica and purple perilla, more
It is important that the effect of product of the present invention prevention and treatment Asparagus Stem Blight can be remarkably promoted.
Specific embodiment
In the examples below, Chinese herbal medicine activity extract is prepared by following technique: will remove deimpurity Chinese herbal medicine
Raw material is crushed, is sieved with 100 mesh sieve in 7 parts of 25 parts of the root of fangji, 18 parts of Artemisia japonica, purple perilla accurate compatibilities of ratio, is added 20 times of herbal raw material
Pure water, be added 5% cellulase of herbal raw material mass ratio, 3% zytase, controlled under the conditions of ultrasonic wave booster action
Warm 50-60 DEG C of enzymatic hydrolysis 6h；It is filtered after enzymatic hydrolysis, filtrate is Chinese herbal medicine activity extract.
In following embodiments, the molecular weight of chitosan oligosaccharide is in 1500Da or so；Compound alga oligosaccharides refer to brown alga oligose
With the mixture of carrageenan oligosaccharide, mass ratio 8:2.
Embodiment 1
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, be made of the raw material of following weight fraction: 15 parts of chitosan oligosaccharide is answered
Close 7 parts of alga oligosaccharides, 40 parts of Chinese herbal medicine activity extract, 11 parts of surfactant, 300 parts of water；
A kind of preparation method of the composition of above-mentioned efficiently prevention and treatment Asparagus Stem Blight, comprising the following steps:
1) chitosan oligosaccharide, compound alga oligosaccharides, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), compound alga oligosaccharides, surfactant are put into the water of formula ratio, stirring
To being completely dissolved；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, obtains efficiently
The composition for preventing and treating Asparagus Stem Blight；
The composition application method of above-mentioned efficient prevention and treatment Asparagus Stem Blight is to be diluted with water to 30 times of liquid to carry out foliar spray.
Embodiment 2
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, be made of the raw material of following weight fraction: 17 parts of chitosan oligosaccharide is answered
Close 6 parts of alga oligosaccharides, 45 parts of Chinese herbal medicine activity extract, 10 parts of surfactant, 350 parts of water；
Preparation method and application method is the same as embodiment 1.
Embodiment 3
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, be made of the raw material of following weight fraction: 13 parts of chitosan oligosaccharide is answered
Close 8 parts of alga oligosaccharides, 38 parts of Chinese herbal medicine activity extract, 12 parts of surfactant, 350 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 1 (contain only Chinese herbal medicine activity extract, the other the same as in Example 1) in composition
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, be made of the raw material of following weight fraction: Chinese herbal medicine activity mentions
Take 62 parts of object, 11 parts of surfactant, 300 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 2 (contain only chitosan oligosaccharide, the other the same as in Example 1) in composition
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, is made: 62 parts of chitosan oligosaccharide, table of the raw material of following weight fraction
11 parts of face activating agent, 300 parts of water；
Preparation method and application method is the same as embodiment 1.
Comparative examples 3 (contain only compound alga oligosaccharides, the other the same as in Example 1) in composition
A kind of composition of efficient prevention and treatment Asparagus Stem Blight, is made of the raw material of following weight fraction: 62 parts of brown alga oligose,
11 parts of surfactant, 300 parts of water；
Preparation method and application method is the same as embodiment 1.
Embodiment 4 compares influence of the different components to Asparagus Stem Blight control efficiency and target for increased production
1, disease survey
Every cell is using 5 points of diagonal line samplings, and every takes connected 5 clumps of asparagus, and 3 asparagus of every clump of asparagus random searching are total
25 clumps of asparagus, 75 asparagus are investigated, healthy tree number and diseased plant number at different levels are recorded.Mark of the severity Scaling standard referring to formulations such as Yang Yingqing
It is quasi-.0 grade: without morbidity；1 grade: onset area accounts for 6% or less the gross area；2 grades: onset area accounts for the 6%~10% of the gross area；3
Grade: onset area accounts for the 11%~20% of the gross area；4 grades: onset area accounts for the 21%~30% of the gross area；5 grades: onset area
Account for 30% or more of the gross area；
2, data processing calculation method
Disease index=∑ (diseased plant numbers at different levels × opposite value of series)/investigation total strain number × 5 × 100；
Control efficiency=(check plot disease index-treatment region disease index)/check plot disease index × 100%；
Rate of growth=(treatment region yield-blank district yield)/blank district yield × 100%；
3, experimental result
1 different components of table are prevented and treated stem wilt and the influence of target for increased production
| Testing index | Preventive effect (%) | Rate of growth (%) |
| 1 test group of embodiment | 90.23±1.21 | 18.22±1.25 |
| 2 test group of embodiment | 88.32±1.62 | 17.45±1.19 |
| 3 test group of embodiment | 89.24±1.18 | 18.48±1.32 |
| 1 group of comparative examples | 29.63±1.09 | 11.45±0.94 |
| 2 groups of comparative examples | 25.18±1.72 | 9.18±0.58 |
| 3 groups of comparative examples | 20.23±1.41 | 12.77±1.12 |
As shown in table 1, test result shows that compared with control group routine operation, embodiment 1-3 prevents Asparagus Stem Blight
Effect illustrates that product of the present invention has significant control efficiency to Asparagus Stem Blight 88% or more.During comparative examples 1 contain only
Herbal medicine activity extract is 29.63% to Asparagus Stem Blight preventive effect maximum value, illustrates Chinese herbal medicine activity extract in spite of one
Determine prophylactic-therapeutic effect, but effect is not significant；Comparative examples 2 contain only chitosan oligosaccharide, are free of Chinese herbal medicine activity extract and compounding sea
Algae oligosaccharides is 25.18% to stem wilt preventive effect, but difference is more significant compared with embodiment 1-3；Comparative examples 3 contain only brown
Algae oligosaccharides is free of Chinese herbal medicine activity extract and chitosan oligosaccharide, is 20.23% to stem wilt preventive effect, but compared with embodiment 1-3
Difference is more significant；Comparative example 1-3 is comprehensively compared to the control efficiency of Asparagus Stem Blight, illustrates chitosan oligosaccharide, alga oligosaccharides and medium-height grass
There is significant synergistic function between medicine activity extract, the skill of " 1+1 > 2 " is achieved in the prevention and treatment to Asparagus Stem Blight
Art effect.Meanwhile experimental result also shows that product of the present invention has remarkable efficacy for promoting Yield of Asparagus, changes products material
Composition can significantly reduce growth promotion effect (P < 0.01) of product.
The above examples are only used to illustrate the technical scheme of the present invention, rather than is limited；Although referring to aforementioned reality
It applies example to be described in detail to by invention, but for those of ordinary skill in the art, it still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features；And to these modifications
Or replacement, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight, it is characterised in that the composition by following parts by weight raw material
Composition: 10-20 parts of chitosan oligosaccharide, alga oligosaccharides 5-10 parts compound, 30-50 parts of Chinese herbal medicine activity extract, surfactant 8-15
Part, 200-400 parts of water.
2. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that the composition
It is made of the raw material of following weight fraction: 13-17 parts of chitosan oligosaccharide, alga oligosaccharides 6-8 parts compound, Chinese herbal medicine activity extract 38-
45 parts, 10-12 parts of surfactant, 250-350 parts of water.
3. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that the composition
It is made of the raw material of following weight fraction: 15 parts of chitosan oligosaccharide, 7 parts of compound alga oligosaccharides, 40 parts of Chinese herbal medicine activity extract, surface
11 parts of activating agent, 300 parts of water.
4. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that the shell is few
The molecular weight of sugar is 1000-2000Da, wherein especially best with the chitosan oligosaccharide of 1500Da molecular weight.
5. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that described compound
Alga oligosaccharides refer to the mixture of brown alga oligose and carrageenan oligosaccharide, mass ratio 8:2；The alga oligosaccharides are to digest widow
Sugar is advisable.
6. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1 to 3, it is characterised in that the medium-height grass
Medicine activity extract is the water extract of the root of fangji, Artemisia japonica, purple perilla, and wherein the proportion compatibility of herbal raw material is 25 parts of the root of fangji, male
18 parts of wormwood artemisia, 7 parts of purple perilla.
7. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 6, it is characterised in that the Chinese herbal medicine
Activity extract is prepared via a method which: deimpurity herbal raw material will be gone to crush, sieved with 100 mesh sieve, it is former that Chinese herbal medicine is added
5% cellulase of herbal raw material mass ratio, 3% zytase is added, in ultrasonic wave booster action item in the pure water of 20 times of material
50-60 DEG C of enzymatic hydrolysis 6h of temperature control under part；It is filtered after enzymatic hydrolysis, filtrate is Chinese herbal medicine activity extract.
8. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that preparation process packet
Include following steps:
1) chitosan oligosaccharide, compound alga oligosaccharides, Chinese herbal medicine activity extract, surfactant and the water of formula ratio are weighed；
2) the load weighted chitosan oligosaccharide of step 1), compound alga oligosaccharides, surfactant are put into the water of formula ratio, is stirred to complete
Fully dissolved；
3) it in the solution for preparing the Chinese herbal medicine activity extract investment step 2) of formula ratio, stirs evenly, is efficiently prevented and treated
The composition of Asparagus Stem Blight.
9. a kind of composition of efficiently prevention and treatment Asparagus Stem Blight according to claim 1, it is characterised in that described efficiently anti-
The composition for controlling Asparagus Stem Blight is diluted with water to 20-30 times of liquid and carries out foliar spray.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811567149.2A CN109452319B (en) | 2018-12-19 | 2018-12-19 | Composition for efficiently preventing and treating stem blight of asparagus and application thereof |
| BE20195611A BE1026953B1 (en) | 2018-12-19 | 2019-09-16 | Composition to fight effectively against the dieback of asparagus stems, and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811567149.2A CN109452319B (en) | 2018-12-19 | 2018-12-19 | Composition for efficiently preventing and treating stem blight of asparagus and application thereof |
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| CN109452319A true CN109452319A (en) | 2019-03-12 |
| CN109452319B CN109452319B (en) | 2021-04-23 |
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| CN109020727A (en) * | 2018-10-12 | 2018-12-18 | 江西省农业科学院植物保护研究所 | A kind of bio-organic fertilizer and preparation method thereof preventing Asparagus Stem Blight |
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| ES2389343B2 (en) * | 2011-03-28 | 2013-06-06 | Bodegas Vega Sicilia S.A. | USE OF NATURAL ANTIFUNGALS TO PREVENT INFECTION OF INJURIES, GRAFTING AND PRUNING WOUNDS IN WOODY PLANTS BY PHYTOPATHOGEN FUNGI. |
| US20150030706A1 (en) * | 2012-03-13 | 2015-01-29 | Consejo Superior De Investigaciones Cientificas (Csic) | Use of essential oils, supercritical extracts and aqueous residues generated during a process for obtaining organic extracts from the artemisia absinthium l. plant |
| MA20150340A1 (en) * | 2014-03-28 | 2015-10-30 | Mascir Moroccan Foundation For Advanced Science Innovation & Res | Composition based on microalgae to stimulate the growth and defense of plants |
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| CN108902160A (en) * | 2018-08-09 | 2018-11-30 | 江西省农业科学院植物保护研究所 | A kind of bactericidal composition and fungicide for preventing and treating Asparagus Stem Blight |
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| CN109020727A (en) * | 2018-10-12 | 2018-12-18 | 江西省农业科学院植物保护研究所 | A kind of bio-organic fertilizer and preparation method thereof preventing Asparagus Stem Blight |
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| BE1026953A1 (en) | 2020-08-04 |
| BE1026953B1 (en) | 2020-08-17 |
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