CN103190400A - New application of sodium butyrate for improving resistance of plant to powdery mildew - Google Patents
New application of sodium butyrate for improving resistance of plant to powdery mildew Download PDFInfo
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- CN103190400A CN103190400A CN2013100840866A CN201310084086A CN103190400A CN 103190400 A CN103190400 A CN 103190400A CN 2013100840866 A CN2013100840866 A CN 2013100840866A CN 201310084086 A CN201310084086 A CN 201310084086A CN 103190400 A CN103190400 A CN 103190400A
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Abstract
The invention discloses a new application of sodium butyrate for improving the resistance of a plant to powdery mildew. The experiment comprises the following steps: applying 100 mmol/L of sodium butyrate aqueous solution during the wheat seed germination period; transplanting the germinated seed in nutrient soil for cultivating; irrigating 100 mmol/L of the sodium butyrate aqueous solution into the soil during the cultivating period; and inoculating erysiphe graminis on the wheat plant with one leaf and one core and then cultivating for 7 days, wherein the result shows that spores or mycelia are not generated on the leaves of the plant, but in other three treatments (applying 100 mM of the sodium butyrate aqueous solution only after the transplanting or only after the germination period, and not applying the sodium butyrate aqueous solution during the germination period and after the transplanting), the sori in different degrees are generated on the leaves of the plant. Therefore, a new way for improving the resistance of the plant to the powdery mildew or preventing and treating the powdery mildew is provided, thereby playing an important significance for the application development of sodium butyrate and the improvement of the resistance of the plant to the powdery mildew or the prevention and the treatment of the powdery mildew.
Description
Technical field
The present invention relates to sodium butyrate and improving plant to the new purposes in the powder mildew resistance.
Background technology
Wheat is one of important crops in the world, and the people in the whole world about 40% is staple food with the wheat.In China, the second largest subsistence crop of wheat, sowing area and output account for about 25% and 22% of grain aggregate respectively throughout the year, and wheat yield and quality are directly connected to national food safety and people's living standard.Along with the popularization of short bar and semi-dwarf mutant wheat breed and the improvement of water and fertilizer condition, the increasing of planting density, cause the wheatland closing, make powdery mildew serious day by day in the harm that China causes, onset area and scope constantly enlarge, rise to main disease by less important disease, the grain-production of China and safety have been caused serious threat.
(Sodium Butyrate NaB) is a kind of white or off-white powder to sodium butyrate, and the become sour fat stink of sample of special cheese is arranged.Sodium butyrate has been widely used in the preparation of feed, feed addictive and the attractant of livestock.Sodium butyrate is a kind of histon deacetylase (HDAC) inhibitor, can improve the acetylation of histone level, and the cancer cell in the mammal and tumour cell are had inhibitory action.At present, less about the research of sodium butyrate effect in the plant, about sodium butyrate the disease resistance of plant especially influence of white powder germ resistance is not appeared in the newspapers as yet yet.
Summary of the invention
The purpose of this invention is to provide sodium butyrate and improving plant to the new purposes in the resistance of powdery mildew or the control plant powdery mildew.Described plant specifically can be wheat.
The present invention also provides a kind of plant powdery mildew bacterium (Blumeria graminis f.sp.tritici) bacteriostatic agent, and the active ingredient of described bacteriostatic agent is sodium butyrate.Described bacteriostatic agent specifically can be the sodium butyrate aqueous solution of 100mmol/L.Described plant specifically can be wheat.
The present invention also provides a kind of and has improved plant to the method for the resistance of powdery mildew or control plant powdery mildew, and described method comprises the step of described plant being used described sodium butyrate or described bacteriostatic agent.
In said method, the described following A of branch that uses) and B) carry out two periods;
A) for from described plant seeding to described plant germination or emerge;
B) for from described plant germination or when emerging to described plant infection white powder germ.
In said method, described plant specifically can be wheat.
In said method, described period A) in, seed and root that described position of using is described plant, the described dosage of using can be the described sodium butyrate of every strain 0.025-0.05 gram;
Described period B) in, described position of using is described plant roots, be the leaf of described plant during one heart stage when the period of described plant infection white powder germ (Blumeria graminis f.sp.tritici), the described dosage of using can be the described sodium butyrate of every strain 0.05-0.15 gram.
In said method, described period A) in, the described mode of using can be immersion, the working concentration of sodium butyrate can be 100mmol/L during described immersion;
Described period B) in, the described mode of using can be pouring, the working concentration of sodium butyrate can be 100mmol/L during described pouring.
Experimental results show that, during wheat seed germinating, use the 100mmol/L sodium butyrate aqueous solution, and cultivate transplanting after the germination in nutrition soil, in soil, water the 100mmol/L sodium butyrate aqueous solution during this time, at a leaf one heart stage inoculation white powder germ biological strain E09(Blumeria graminis f.sp.tritici) the back cultivation 7 days, the plant leaf surface does not all have spore or mycelia produces, and other three kinds processing (are only used the 100mM sodium butyrate aqueous solution after transplanting, only use the 100mM sodium butyrate aqueous solution during germination, duration of germination and transplant after all do not use sodium butyrate) the plant leaf surface all have sorus in various degree to produce.The present invention provides a kind of new approach for resistance or the control plant powdery mildew that improves plant powdery mildew, to the application and development of sodium butyrate with improve the plant powdery mildew resistance or the control plant powdery mildew has great importance.
Description of drawings
Fig. 1 is that four kinds of wheat plants under the different disposal are to the resistant phenotype of white powder germ.Wherein, the blade phenotype after A, B, C, D are respectively treated A, treatments B, processing C and handle D behind the inoculation white powder germ.
Embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Experiment material information used among the following embodiment is as follows:
Wheat breed agricultural university 189: document: Xie Hao. the molecular labeling of wheat external source mildew-resistance gene. the thesis for the doctorate .2005 of China Agricultural University. the public can obtain from China Agricultural University.
Wheat breed China spring: document: Tang Yuan, Fu Tihua, the new wheat powdery mildew resistant gene in Jia Ming beautiful .SSR mark location. North China agronomy newspaper, 2008,23(5): 127-130.The public can obtain from China Agricultural University.
The wheat breed capital winter No. 8: Tongzhou District, Beijing City seeds company.
Wheat breed Xue is (common wheat of easy infection wheat powdery mildew) early: document: Zhang Liansong, Huawei, Guan Haiying, Li Genqiao, Zhang Hongtao, Xie Chaojie, Yang Zuomin, Sun Qixin, Liu Zhiyong. wild emmer imports the mildew-resistance gene MlWE29 molecular labeling location of common wheat.Acta Agronomica Sinica 2009,35 (6): 998-1005.The public can obtain from China Agricultural University.
Wheat breed capital 411: document: in shaking Li Qian, Zhao Jianye etc. the variation of protein group behind cultivated wheat Brock and the capital 411 infection powdery mildews. Acta Agronomica Sinica .2009,35 (11): 2064-2072.The public can obtain from China Agricultural University.
White powder germ biological strain E09(Blumeria graminis f.sp.tritici): document: Zhang Liansong, Huawei, Guan Haiying, Li Genqiao, Zhang Hongtao, Xie Chaojie, Yang Zuomin, Sun Qixin, Liu Zhiyong. wild emmer imports the mildew-resistance gene MlWE29 molecular labeling location of common wheat. Acta Agronomica Sinica .2009,35 (6): 998-1005.
Sodium butyrate (C
4H
7O
2Na): molecular weight 110.09, purity 98%, Sigma company produces.
Embodiment 1, sodium butyrate are improving plant to the application in the resistance of powdery mildew or the control plant powdery mildew
One, experimental technique
Get wheat breed agricultural university 189, China spring, capital winter No. 8, Xue's morning and capital 411 seeds, after carrying out following four kinds of processing (A, B, C and D) respectively, identify plant to the resistance of powdery mildew, (handle each wheat breed for every kind and all establish 3 repetitions, each repeats 15-20 seeds) specific as follows:
Handle A: get wheat seed and place and contain 50-100ml
The 100mmol/L sodium butyrate aqueous solutionGermination paper ((contain 20 seeds) in 30 * 15cm), day alternates with night in 20 ℃, 12 hours/12 hours and 75% intensity of illumination under germinate and cultivated 7 days; Seeds germinated transplanted (nutrition soil is 1:2 with the mixed proportion of vermiculite in containing soil, wherein nutrition soil is the flower nutrition soil that virtue clean colored Tu Chang in Dehui City, Jilin Province produces, composition is needle, broad-leaved sod deposit, processing peat and vermiculite etc.) small flower in (following liner tray), 15-20 seeds of every basin, 20 ℃ of 24 ℃ by day, night, 12 hours/day alternates with night in 12 hours, cultivate under humidity 90% and 75% intensity of illumination, during in nutrition soil, use
100mmol/L The sodium butyrate aqueous solution(each 50ml used once in per 2-3 days, and every basin is used 100ml-200ml altogether and make plant absorbing in the basin to pallet before inoculation white powder germ); At plant strain growth to a leaf during one heart stage, with white powder germ biological strain E09(Blumeria graminis f.sp.tritici) numerous bacterium basin places around the seedling, white powder germ spore is inoculated the seedling of plant to be measured by natural propagation, observes the incidence of plant after 15 days.The result as shown in Figure 1.
Treatments B: and the difference of handling A is: seeds germinated is the water that does not contain sodium butyrate transplant what use between the culture period behind the nutrition soil; Specific as follows:
Getting wheat seed places and contains 50-100ml1
The 00mmol/L sodium butyrate aqueous solutionGermination paper ((contain 20 seeds) in 30 * 15cm), day alternates with night in 20 ℃, 12 hours/12 hours and 75% intensity of illumination under germinate and cultivated 7 days; Seeds germinated transplanted (nutrition soil is 1:2 with the mixed proportion of vermiculite in containing soil, wherein nutrition soil is the flower nutrition soil that virtue clean colored Tu Chang in Dehui City, Jilin Province produces, composition is needle, broad-leaved sod deposit, processing peat and vermiculite etc.) small flower in (following liner tray), 15-20 seeds of every basin, 20 ℃ of 24 ℃ by day, night, 12 hours/day alternates with night in 12 hours, cultivate under humidity 90% and 75% intensity of illumination, during in nutrition soil, use
Do not contain The water of sodium butyrate(each 50ml used once in per 2-3 days, and every basin is used 100ml-200ml altogether and make plant absorbing in the basin to pallet before inoculation white powder germ); At plant strain growth to a leaf during one heart stage, with white powder germ biological strain E09(Blumeria graminis f.sp.tritici) numerous bacterium basin places around the seedling, white powder germ spore is inoculated the seedling of plant to be measured by natural propagation, observes the incidence of plant after 15 days.
Handle C: and the difference of handling A is: germinate and be the water that does not contain sodium butyrate transplant what use between culture period after the germination in nutrition soil with the water that does not contain sodium butyrate; Specific as follows:
Get wheat seed place the germination paper that contains 50-100ml and do not contain the water of sodium butyrate (30 * 15cm) (containing 20 seeds), day alternates with night in 20 ℃, 12 hours/12 hours and 75% intensity of illumination under germinate and cultivated 7 days; Seeds germinated transplanted (nutrition soil is 1:2 with the mixed proportion of vermiculite in containing soil, wherein nutrition soil is the flower nutrition soil that virtue clean colored Tu Chang in Dehui City, Jilin Province produces, composition is needle, broad-leaved sod deposit, processing peat and vermiculite etc.) small flower in (following liner tray), 15-20 seeds of every basin, 20 ℃ of 24 ℃ by day, night, 12 hours/day alternates with night in 12 hours, cultivate under humidity 90% and 75% intensity of illumination, during in nutrition soil, use
The sodium butyrate that does not contain Water(each 50ml used once in per 2-3 days, and every basin is used 100ml-200ml altogether and make plant absorbing in the basin to pallet before inoculation white powder germ); At plant strain growth to a leaf during one heart stage, with white powder germ biological strain E09(Blumeria graminis f.sp.tritici) numerous bacterium basin places around the seedling, white powder germ spore is inoculated the seedling of plant to be measured by natural propagation, observes the incidence of plant after 15 days.
Handle D: be with the difference of handling A: germinate with the water that does not contain sodium butyrate.Specific as follows:
Getting wheat seed places and contains 50-100ml
The water that does not contain sodium butyrateGermination paper ((contain 20 seeds) in 30 * 15cm), day alternates with night in 20 ℃, 12 hours/12 hours and 75% intensity of illumination under germinate and cultivated 7 days; Seeds germinated transplanted (nutrition soil is 1:2 with the mixed proportion of vermiculite in containing soil, wherein nutrition soil is the flower nutrition soil that virtue clean colored Tu Chang in Dehui City, Jilin Province produces, composition is needle, broad-leaved sod deposit, processing peat and vermiculite etc.) small flower in (following liner tray), 15-20 seeds of every basin, 20 ℃ of 24 ℃ by day, night, 12 hours/day alternates with night in 12 hours, cultivate under humidity 90% and 75% intensity of illumination, during in nutrition soil, use
The 100mmol/L butyric acid Sodium water solution(each 50ml used once in per 2-3 days, and every basin is used 100ml-200ml altogether and make plant absorbing in the basin to pallet before inoculation white powder germ); At plant strain growth to a leaf during one heart stage, with white powder germ biological strain E09(Blumeria graminis f.sp.tritici) numerous bacterium basin places around the seedling, white powder germ spore is inoculated the seedling of plant to be measured by natural propagation, observes the incidence of plant after 15 days.
Two, experimental result
Wheat breed agricultural university 189, China spring, capital winter No. 8, Xue's morning and capital 411 are after four kinds of processing (A, B, C and D) of step 1, the plant incidence as shown in Figure 1, after being treated A, the plant leaf surface is no spore or mycelia generation all, and the plant leaf surface all has sorus in various degree to produce behind other three kinds of treatments B, C and D.
Claims (10)
1. sodium butyrate is improving plant to the application in the resistance of powdery mildew or the control plant powdery mildew.
2. application according to claim 1 is characterized in that: described plant is wheat.
3. a plant powdery mildew bacterium (Blumeria graminis f.sp.tritici) bacteriostatic agent, it is characterized in that: the active component of described bacteriostatic agent is sodium butyrate.
4. bacteriostatic agent according to claim 2, it is characterized in that: described bacteriostatic agent is the sodium butyrate aqueous solution of 100mmol/L.
5. according to claim 3 or 4 described bacteriostatic agents, it is characterized in that: described plant is wheat.
6. one kind is improved plant to the method for the resistance of powdery mildew or control plant powdery mildew, and it is characterized in that: described method comprises the step of described plant being used described sodium butyrate or claim 2 or 3 described bacteriostatic agents.
7. method according to claim 6 is characterized in that: the described following A of branch that uses) and B) carry out two periods;
A) for from described plant seeding to described plant germination or emerge;
B) for from described plant germination or emerge to described plant infection white powder germ (Blumeria graminis f.sp.tritici).
8. according to claim 6 or 7 described methods, it is characterized in that: described plant is wheat.
9. method according to claim 8 is characterized in that:
Described period A) in, seed and root that described position of using is described plant, the described dosage of using are the described sodium butyrate of described plant 0.025-0.05 gram of every strain;
Described period B) in, described position of using is described plant roots, be the leaf of described plant during one heart stage when the period of described plant infection white powder germ (Blumeria graminis f.sp.tritici), the described dosage of using is the described sodium butyrate of described plant 0.05-0.15 gram of every strain.
10. it is characterized in that according to Claim 8 or 9 described methods:
Described period A) in, the described mode of using is for soaking, the working concentration of sodium butyrate is 100mmol/L during described the immersion;
Described period B) in, the described mode of using is pouring, the working concentration of sodium butyrate is 100mmol/L during described pouring.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904714A (en) * | 2015-06-18 | 2015-09-16 | 青岛农业大学 | Application of sodium butyrate in preparation of fungicide used for preventing and controlling plant diseases caused by phytopathogens |
| CN105861291A (en) * | 2016-04-15 | 2016-08-17 | 湖北省农业科学院植保土肥研究所 | Simple and convenient inoculating device for blumeria graminis f.sp.tritici and application method of inoculating device |
| CN106472595A (en) * | 2016-09-19 | 2017-03-08 | 德州市农业科学研究院 | A kind of biological agent of preventing and treating wheat powdery mildew and preparation method thereof |
| CN108013059A (en) * | 2017-12-12 | 2018-05-11 | 中国农业大学 | It is a kind of to strengthen processing method of the wheat to powder mildew resistance |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0576199A2 (en) * | 1992-06-25 | 1993-12-29 | Rohm And Haas Company | Fungicidal and herbicidal triazoles |
| EP1815742A1 (en) * | 2004-11-02 | 2007-08-08 | Asahi Breweries, Ltd. | Agent for elevating plant disease-resistane and method of producing the same |
-
2013
- 2013-03-15 CN CN201310084086.6A patent/CN103190400B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0576199A2 (en) * | 1992-06-25 | 1993-12-29 | Rohm And Haas Company | Fungicidal and herbicidal triazoles |
| EP1815742A1 (en) * | 2004-11-02 | 2007-08-08 | Asahi Breweries, Ltd. | Agent for elevating plant disease-resistane and method of producing the same |
Non-Patent Citations (3)
| Title |
|---|
| 张倩: "丁酸钠在动物生产中的应用研究进展", 《中国饲料》, no. 11, 5 June 2009 (2009-06-05), pages 28 - 30 * |
| 杜亚平，等: "丁酸钠溶液灌肠对大鼠内脏感觉的影响", 《胃肠病学》, vol. 13, no. 2, 25 February 2008 (2008-02-25), pages 91 - 94 * |
| 阳欣科，于成功: "丁酸钠抑制淋巴因子激活杀伤细胞杀瘤活性的研究", 《医学研究生学报》, vol. 18, no. 5, 20 May 2005 (2005-05-20), pages 393 - 396 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904714A (en) * | 2015-06-18 | 2015-09-16 | 青岛农业大学 | Application of sodium butyrate in preparation of fungicide used for preventing and controlling plant diseases caused by phytopathogens |
| CN105861291A (en) * | 2016-04-15 | 2016-08-17 | 湖北省农业科学院植保土肥研究所 | Simple and convenient inoculating device for blumeria graminis f.sp.tritici and application method of inoculating device |
| CN105861291B (en) * | 2016-04-15 | 2017-12-08 | 湖北省农业科学院植保土肥研究所 | The easy classification inoculation apparatus and its application method of wheat powdery mildew |
| CN106472595A (en) * | 2016-09-19 | 2017-03-08 | 德州市农业科学研究院 | A kind of biological agent of preventing and treating wheat powdery mildew and preparation method thereof |
| CN106472595B (en) * | 2016-09-19 | 2018-10-23 | 德州市农业科学研究院 | A kind of biological agent and preparation method thereof of prevention wheat powdery mildew |
| CN108013059A (en) * | 2017-12-12 | 2018-05-11 | 中国农业大学 | It is a kind of to strengthen processing method of the wheat to powder mildew resistance |
| CN108013059B (en) * | 2017-12-12 | 2020-05-01 | 中国农业大学 | Treatment method for enhancing resistance of wheat to powdery mildew |
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