WO2023155853A1

WO2023155853A1 - Use of 3-methyl-2-methylamino valeric acid in promoting plant growth

Info

Publication number: WO2023155853A1
Application number: PCT/CN2023/076582
Authority: WO
Inventors: 陈世国; 王赫; 王良省; 房婉萍; 郭爱平
Original assignee: 南京天秾生物技术有限公司
Priority date: 2022-02-18
Filing date: 2023-02-16
Publication date: 2023-08-24
Also published as: CN114514932A

Abstract

Provided is the use of a 3-methyl-2-methylamino valeric acid in promoting plant growth. A 3-methyl-2-methylamino valeric acid, as a natural active substance, is developed into a plant growth regulator which can be used for promoting the growth of plants and can remarkably increase the plant height, root length, number of lateral roots, true leaf area, plant fresh weight and the like of plants. The 3-methyl-2-methylamino valeric acid has the characteristics of safety, environmental protection and high efficiency.

Description

Application of 3-methyl-2-methylaminovaleric acid in promoting plant growth

technical field

The invention belongs to the field of agricultural biological pesticides and relates to the application of 3-methyl-2-aminovaleric acid in promoting plant growth.

Background technique

Plant growth regulators refer to artificially synthesized or extracted compounds with natural plant hormone physiological activity, which can be used to regulate or control certain processes of plant growth and development, such as cell division and elongation, tissue and organ differentiation, seed dormancy and germination, Flowering and fruiting, maturity and aging, etc., to promote or inhibit seed germination, plant growth, fruit ripening, flower preservation or fruit thinning, improve plant immunity, help plants resist adverse environments, reduce diseases, and increase crop yields , Improve crop quality and other purposes. Because of its significant and efficient regulating effect, it has been widely used in various aspects such as grain, fruit trees, forest trees, vegetables, and flowers. At present, my country has become one of the countries where plant growth regulators are widely used in the world.

Plant growth regulators have played a huge role in regulating crop growth, solved many problems that cannot be solved by traditional agronomic means, and made important contributions to my country's agricultural production and development. One of the main measures. However, the food safety problems caused by the blind and excessive use of plant growth regulators still exist widely, and the international standards for the residue limit of plant growth regulators are becoming more and more stringent. Therefore, it is very important to develop low-toxicity, high-efficiency, and environment-friendly natural plant growth regulators to ensure green and safe agricultural production.

3-Methyl-2-methylaminovaleric acid, the molecular formula is C ₇ H ₁₅ NO ₂ , the molecular weight is 145 g/mol, and it is a colorless crystal. It is a structural component of a variety of natural products. Fusarin A (Plattner & Nager, 1948), indole lactams produced by Streptomyces and Nocardiopsis (Irie et al., 1990; Sun et al., 1991) and cyanobacterial cyclic The structures of peptides (Luo et al., 2014) all contain 3-methyl-2-methylaminovaleric acid. In 1996, Li et al. successfully isolated 3-methyl-2-aminovaleric acid from the seeds of Phaseolus vulgaris (Li et al., 1996). Recently, we successfully isolated and purified 3-methyl-2-aminovaleric acid from the fungus Alternaria sp. Up to now, there are no studies, reports or patents related to the plant growth promoting activity of this compound.

Contents of the invention

The object of the present invention is to address the above-mentioned deficiencies of the prior art, and to provide the application of 3-methyl-2-aminovaleric acid (I) as a growth regulator to promote plant growth.

The purpose of the present invention can be achieved through the following technical solutions:

Application of 3-methyl-2-methylaminovaleric acid in promoting the growth of plant seedlings.

As a preference of the present invention, the application of 3-methyl-2-methylaminovaleric acid to promote the occurrence and growth of root system of plant seedlings through hydroponic treatment.

As a preference of the present invention, the application of 3-methyl-2-methylaminovaleric acid to promote the growth of plant seedlings through root irrigation treatment.

As a preference of the present invention, the plant is preferably cucumber.

Application of 3-methyl-2-methylaminovaleric acid in the preparation of plant growth regulators.

As a preference of the present invention, the plant is preferably cucumber.

The existing related research on 3-methyl-2-methylaminovaleric acid does not involve reports in the field of plant growth regulators. my country is one of the major countries in the application of plant growth regulators. As the public pays more and more attention to food safety and health, it is particularly urgent to develop low-toxicity, high-efficiency, and environment-friendly plant growth regulators. Therefore, the development of natural plant growth regulators and the promotion of their industrialization are of great significance for ensuring food safety and improving the competitiveness of agricultural products. 3-methyl-2-methylaminovaleric acid performed well in related experiments on promoting plant growth, and could significantly promote plant growth.

A method for improving plant growth, characterized in that 10-1000nM of 3-methyl-2-aminovaleric acid is applied to target plants.

As a preferred method of the present invention, the natural product 3-methyl-2-aminovaleric acid is used to promote the rooting of cucumber seedlings. It can significantly promote Cucumber seedling growth; especially when the concentration was 100nM, compared with the blank control, the root length, lateral root number, root fresh weight and whole plant fresh weight of cucumber increased by 205%, 69%, 167% and 54% respectively.

As a preferred method of the present invention, 3-methyl-2-methylaminovaleric acid is used to promote the growth of cucumber seedlings. When the concentration is 100nM, the root irrigation method is used to treat cucumber soil-cultured seedlings, which can significantly promote the growth of cucumber seedlings. Growth; compared with the blank control group, plant height, leaf area of the first true leaf, leaf area of the second true leaf, The fresh weight of the whole plant, the fresh weight of the aboveground part, the fresh weight of the underground part and the root length increased by 22%, 62%, 94%, 54%, 40%, 132% and 48% respectively.

Technological advances and beneficial effects

Main advantage of the present invention and positive effect are as follows:

3-Methyl-2-methylaminovaleric acid is a natural product with a simple structure and easy industrial production. The present invention confirms that 3-methyl-2-methylaminovaleric acid can promote plant growth, and 3-methyl-2-methylaminovaleric acid can promote the growth and development of plants by doing hydroponics and root watering treatment, and can significantly improve the growth and development of plants. Plant height, root length, number of lateral roots, true leaf area, plant fresh weight, etc. 3-methyl-2-aminovaleric acid is a natural product with low dosage, high environmental and biological safety, and has the potential to be developed into a natural Potential as a plant growth regulator.

Detailed ways

The inventor conducted research on the biological activity and crop safety of 3-methyl-2-methylaminovaleric acid, and found that the substance has a very unique role in regulating crop growth, and has the advantages of environmental friendliness, high activity, and safe use. , has the potential to be developed as a biopesticide. The substantive features of the present invention can be realized from the following embodiments and examples, but these should not be regarded as any limitation to the invention.

Example 1: Effect of 3-methyl-2-aminovaleric acid on rooting of cucumber seedlings

In order to study the effect of 3-methyl-2-aminovaleric acid on the growth of plant roots, a model system for studying lateral root development—the radicleless cucumber seedling system was selected. Spread four layers of wet gauze on the bottom of the culture box, select cucumber seeds with plump shape and uniform size (the variety is "Chuxia Qiuguan", Shandong Ningyang County Luming Seed Co., Ltd.), disinfect with 75% alcohol, and rinse with distilled water Sprinkle it evenly on the gauze, and then cover it with four layers of wet gauze to avoid light. After being protected from light for 24 hours in a culture room at 25° C., the germinated cucumber seeds were taken out and buried 1 centimeter (cm) below the soil. After 3 days, take some seedlings with the same growth and fully stretched two cotyledons, rinse the roots with distilled water, cut off the radicle along the hypocotyl, fix the plants with sponge strips, and place them in the culture box. Add 400 milliliters (mL) of 3-methyl-2-methylaminovaleric acid aqueous solution with concentrations of 0, 10, 100 and 1000 nM to each box, respectively. The hydroponic plants were placed in a 25°C culture room, and after 6 days of light cultivation, they were taken out to measure and record the root length, the number of lateral roots, the fresh weight of the roots and the fresh weight of the whole plant. The results are shown in Table 1.

Table 1 Effects of different concentrations of 3-methyl-2-aminovaleric acid on rooting of cucumber seedlings

It can be known from Table 1 that 10-1000 nM of 3-methyl-2-aminovaleric acid can all promote the rooting of cucumber seedlings. When the concentration was 10, 100 and 1000nM, compared with the blank control, the root length of cucumber increased by 102%, 205% and 103% respectively; the number of lateral roots increased by 53%, 69% and 54% respectively; Increased by 67%, 167%, and 33%; the fresh weight of the whole plant increased by 39%, 54%, and 22% respectively. Among them, 100nM 3-methyl-2-methylaminovaleric acid has the best treatment effect. The above results indicate that 3-methyl-2-methaminovaleric acid can significantly promote the occurrence and growth of roots of cucumber seedlings.

Example 2: Effects of 3-methyl-2-aminovaleric acid on the growth of cucumber seedlings

Spread four layers of wet gauze on the bottom of the culture box, select cucumber seeds with plump shape and uniform size (the variety is "Chuxia Qiuguan", Shandong Ningyang County Luming Seed Co., Ltd.), disinfect with 75% alcohol, and rinse with distilled water Sprinkle it evenly on the gauze, and then cover it with four layers of wet gauze to avoid light. After being protected from light for 24 hours in a culture room at 25°C, take out the germinated cucumber seeds, bury them in plastic pots (diameter 12 cm) filled with moist (saturated water) soil, with a depth of 2-3 cm, 5 seeds per pot, and place them at 25 Cultivate in a culture room (light intensity 200 μmol m ^-2 s ^-1 ). After 3 days, keep 1 strong seedling with cotyledons fully expanded, and remove the other 4 seedlings. Then, slowly pour 50 mL of 3-methyl-2-aminovaleric acid at a concentration of 0 (blank control) and 100 nM along the base of the seedling stem in each pot, and continue to cultivate under light in a 25° C. culture room. On the 6th and 12th days after treatment, observe and record the plant height and true leaf growth of each cucumber seedling; on the 18th day, measure the plant height, first true leaf area, second true leaf area, and Leaf area, third true leaf area, fresh weight of whole plant, fresh weight of aboveground part, fresh weight of underground part and root length. The results are shown in Table 2.

Table 2 The effect of 3-methyl-2-methylaminovaleric acid on the growth of cucumber plant seedlings

The results in Table 2 show that, compared with the blank control, 3-methyl-2-aminovaleric acid can significantly promote the growth of cucumber seedlings. For example, on the 12th day, the blank control group had not yet grown a second true leaf, while the cucumber seedlings treated with 100 nM 3-methyl-2-methaminovaleric acid had grown 9.74 cm ² leaves respectively. The second true leaf; on the 18th day, the blank control group had not yet grown the third true leaf, while the cucumber seedlings treated with 100 nM 3-methyl-2-aminovaleric acid had already grown leaves. The third true leaf with an area of 4.16 ^cm2 . On the 18th day, compared with the blank control, the plant height, leaf area of the first true leaf, leaf area of the second true leaf, whole Plant fresh weight, aboveground fresh weight, underground fresh weight and root length increased by 22%, 62%, 94%, 54%, 40%, 132% and 48% respectively. This shows that 3-methyl-2-methylaminovaleric acid can significantly promote the development of seedlings, and promote the development and rapid growth of roots, stems and leaves.

Claims

Application of 3-methyl-2-methylaminovaleric acid in the preparation of plant growth regulators.
Use of 3-methyl-2-methylaminovaleric acid in promoting plant growth.
The use according to claim 1 or 2, characterized in that said plants are selected from vegetables. Described vegetables are preferably cucumbers.
A method for improving plant growth, characterized in that 10-1000 nM of 3-methyl-2-aminovaleric acid is applied to target plants.