CN109892228A - A kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling - Google Patents
A kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling Download PDFInfo
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- CN109892228A CN109892228A CN201910343993.5A CN201910343993A CN109892228A CN 109892228 A CN109892228 A CN 109892228A CN 201910343993 A CN201910343993 A CN 201910343993A CN 109892228 A CN109892228 A CN 109892228A
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Abstract
The invention discloses a kind of outside sprout-cultivating-bottle methods of Chinese catalpa tissue-cultured seedling, comprising the following steps: 1) material of taking root is selected；2) it takes root material wound healing；3) tissue-cultured seedling cuttage root-taking；4) nutrition pot seedling is fostered.In the outside sprout-cultivating-bottle method for the Chinese catalpa tissue-cultured seedling that the present invention uses, wound healing processing is carried out to the notch for material of taking root first, subsequent cultivation of taking root is carried out again, so that the tissue of incision can be good at healing, reduce the risk of infection of incisional wound germ, it is subsequent preferably to take root, and shortens entire taking root and cultivate the time, and, Chinese catalpa seedling after taking root can under room temperature natural environment long root, have a large amount of fibrous roots on main root, root activity is strong, nutrition after new root issued in 3-5 days in direct absorption base promotes young leaves pumping, and root survival is high.The present invention is suitable for the outside sprout-cultivating-bottle of Chinese catalpa tissue-cultured seedling.
Description
Technical field
The invention belongs to plant cultivation fields, are related to a kind of method of outside sprout-cultivating-bottle, and in particular to a kind of Chinese catalpa tissue-cultured seedling
Outside sprout-cultivating-bottle method.
Background technique
Chinese catalpa is Bignoniaceae Catalpa deciduous tree, under the conditions of on the spot preferable, sets height up to 30 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is up to 1 meter
More than.Chinese catalpa source area is China, is the distinctive indigenous tree in China, and tree body is tall and big tall and straight, dry-shaped straight, and tree performance is graceful,
Pattern is gorgeous, is tall and big flower trees.Because its material is excellent, therefore it is known as the title of " the wooden king ".Chinese catalpa timber value is high, volume recovery
Greatly.But ready-made for various reasons is endangered species.Chinese catalpa belongs to deep ploughing property tree species, and fibrous root is seldom in earth's surface arable layer, not with agriculture
Crop strives liquid manure, and for prevention soil and water loss, retardance wind erosion, fixed dune, protecting the fields plays a good role.In addition,
Chinese catalpa, which has, to lay the dust, the function of the pernicious gases such as sulfur dioxide absorption, chlorine.
Chinese catalpa is mainly bred using the modes such as sowing, cuttage, grafting, tissue cultures at present.But due to Chinese catalpa by
The influence of its hereditary capacity, seminal propagation has that self-sterility, therefore germination percentage is lower, and characters of progenies separation is tight
Weight.Therefore, to the merit of holding maternal plant, vegetative propagation can only be carried out, wherein the vegetative propagation side of cuttage root-taking
Formula is limited its application since rooting rate is low；Therefore stock is generally made using Chinese catalpa in production, grafting Chinese catalpa carries out Chinese catalpa kind
This method of the breeding of seedling, but the Chinese catalpa seedling of Chinese catalpa grafting, intolerant to continuous cropping, root system easy infection knot nematodes disease is lost once falling ill
Heaviness, and easily there is " size pedopathy ", trees are difficult to become a useful person.By tissue culture technique breed Chinese catalpa be from root system seedling,
There is no the rooting methods of the problem of Chinese catalpa infection knot nematodes disease, but traditional Chinese catalpa tissue-cultured seedling need to undergo in bottle (25- of taking root
30 days), outside sprout-cultivating-bottle hardening (25-30 days), nutrition pot seedling foster (20-30 days) three phases, obtain finished product seedling and need 70-
120 days, the acquisition period of entire finished product seedling was longer.In addition, matrix used by taking root in traditional Chinese catalpa tissue-cultured seedling bottle is additional
The agar medium of sugar, minerals etc., plant leaf do not have photosynthetic capacity, and root system is mostly fleshy root, and in greenhouse, needs pass through
Prolonged hardening adapts it to natural environment, and survival rate is unstable, easily affected by environment.
Therefore, a kind of acquisition time that not only can be shortened finished product seedling, but also the energy higher breeding method of root survival, tool are studied
There is important meaning.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling, both can be shortened into
The acquisition time of product seedling, and root survival can be improved.
In order to solve the above technical problems, the technical solution used in the present invention is:
A kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling, comprising the following steps:
1) it takes root the selecting of material: selecting the Chinese catalpa that plant height is 2~4cm, subculture cycle is 25~35 days, leaf color is dark green
Tissue-cultured seedling is set, radical leaves, axillary bud are cut off, 3~5 blades being fully deployed are stayed at top, obtain materials A of taking root；
2) it takes root material wound healing: the cutting part for materials A of taking root being coated into wound healing agent for plants, later access training
It supports and is cultivated 3~5 days in base M, obtain material B of taking root；
3) tissue-cultured seedling cuttage root-taking: coating nutrient solution N for the notch for material B of taking root, and it is raw that cuttage is carried out in matrix I
Root is cultivated 7~14 days, and rooted seedling C is made；Wherein, the Chinese catalpa cuttage root-taking matrix I include stratiform be laid with perlite,
Turf and vermiculite；
4) nutrition pot seedling is fostered: rooted seedling C being transplanted to equipped in II nutritive cube of matrix, it is raw to obtain Chinese catalpa within culture 20-28 days
Offspring D；Wherein, the matrix II is bi-layer matrix, and wherein overlayer matrix is the vermiculite of 1~2:1:1 of volume ratio: perlite, under
Layer matrix is 1~3:1 of volume ratio~2:1 turf: vermiculite: perlite.
Culture medium M as a kind of restriction of the invention, in the step 2) are as follows: 30-60 mesh vermiculite and deionized water exist
Be made after sterilizing 10min at 121 DEG C, wherein 30-60 mesh vermiculite: the volume ratio of deionized water is=1:1.Wherein, culture medium M
Compared with traditional agar medium, agar is substituted with inorganic matrix vermiculite, and does not need addition nutrients because cultivation cycle is short
Matter can achieve the effect that wound healing, while not influence plant strain growth.Therefore, cleaning is not had to when Chinese catalpa tissue-cultured seedling bottle outlet
It can directly transplant to seedbed, save artificial needed for cleaning seedling.Further, since culture medium is free of nutriment, cultivated
Journey and transplanting are to not easy infection fungi when cultivating behind seedbed.
As further limiting for above-mentioned restriction, the wound healing agent for plants is the mixture of fungicide and adjuvant,
And the concentration for adding deionized water to dilute 800-1500 times for fungicide using concentration of wound healing agent for plants.The fungicide and
The mixability of adjuvant intermixture is can make adhesive mixture on the cutting part of plant.Used in the present invention
Carbendazim is state's light board, and active constituent content 50%, dosage form is the carbendazim of wettable powder, and its chemical name is N- (2-
Benzimidazolyl)-methyl carbamate.
As further limiting for above-mentioned restriction, the fungicide be one of carbendazim or potassium permanganate, it is described auxiliary
Auxiliary agent is one kind of plant ash or pulverized limestone.
It is limited as another kind of the invention, the condition of culture in the step 2) are as follows: 24~28 DEG C of cultivation temperature, illumination
3000~5000Lux of intensity, light irradiation time 10~14h/ days.Plant strain growth under the condition of culture is healthy and strong, and is conducive to cut
Wound healing at mouthful.
It is limited as another kind of the invention, the nutrient solution N in the step 3) is mass/volume than for 1.5~4:1's
Talcum powder: methyl α-naphthyl acetate, wherein the concentration of methyl α-naphthyl acetate is 0.3~1.0mg/L.Wherein, nutrient solution N and fine jade commonly used in the prior art
Rouge culture medium is compared, and the rooting rate of plant cultivated in nutrient solution N is higher.
It is limited as another kind of the invention, the condition of culture in the step 3) are as follows: 22~28 DEG C of air themperature, soil
Temperature is 24~30 DEG C, air humidity >=80%, and illumination condition is 5000~7000Lux.The sky under the condition of culture
The plant leaf cultivated within the scope of temperature degree and air humidity is not in the withered phenomenon of dehydration, and young leaves is sprouted fastly；?
Under the condition of culture, sprouted fastly using the plant root cultivated within the scope of the soil moisture, the speed of growth is fast；In the condition of culture institute
The plant leaf of culture is not easy to burn withered within the scope of the illumination condition stated, and can be carried out photosynthesis appropriate.
It is limited as another kind of the invention, the step 3) mesostroma I includes the perlite of 3~5cm thickness, and 1~3cm is thick
Turf and 1~3cm thickness vermiculite.As further limiting for above-mentioned restriction, lower layer is pearl in the step 3) mesostroma I
Rock, middle layer are turf, and upper layer is vermiculite.Vermiculite in the matrix I it is loose it is ventilative, water-retaining property is stronger, be placed in upper layer and be conducive to plant
Strain new root is sprouted and plant is not easy to lodge；Turf is rich in organic matter, is placed in middle layer conducive to the battalion in turf is absorbed after root elongation
It supports；Perlite it is loose it is ventilative, water-retaining property is strong, and price is lower, is of relatively low cost.
It is limited as another kind of the invention, the condition of culture in the step 4) are as follows: air themperature is 22~28 DEG C；Soil
Earth temperature is 24~30 DEG C；Preceding 7 days air humidity >=80% after transplanting, after gradually to natural humidity；Preceding illumination in 7 days is strong after transplanting
Degree≤10000Lux, after gradually to natural lighting.It is cultivated within the scope of the air themperature described in the condition of culture and air humidity
Plant leaf is not in the withered phenomenon of dehydration, and young leaves is sprouted fastly；Under the condition of culture, soil moisture range is used
The plant root of interior culture is sprouted fastly, and the speed of growth is fast；The plant cultivated within the scope of the illumination condition described in the condition of culture
Blade is not easy to burn withered, and can be carried out photosynthesis appropriate, gradually reform of nature environment.
For the present invention there are also a kind of restriction, the volume ratio of II overlayer matrix of matrix and underlying substrate in the step 4) is 1:
2。
Due to the adoption of the above technical solution, compared with prior art, the present invention acquired technological progress is:
In the outside sprout-cultivating-bottle method for the Chinese catalpa tissue-cultured seedling that the present invention uses, the notch for material of taking root is carried out at healing first
Reason, then subsequent cultivation of taking root is carried out, so that the tissue of incision can be good at healing, it is subsequent preferably to be given birth to
Root, and shorten entire taking root and cultivate the time, when using method bottle internal injury healing of the invention be 3 days, seedbed is taken root
Between be 7-14 days, the time that nutrition pot seedling is fostered is 20-28 days, obtains entire rooted seedling and needs 30-45 days altogether, more existing
The method time shortens.Also, the Chinese catalpa seedling after taking root can under room temperature natural environment long root, have a large amount of fibrous roots on main root,
Root activity is strong, and the nutrition after new root issued in 3-5 days in direct absorption base promotes young leaves pumping, and root survival is higher,
Reach 98% or more, leaf color is dark green, cultivates 25-30 days plant heights up to 6cm or more.
The present invention is suitable for the outside sprout-cultivating-bottle of Chinese catalpa tissue-cultured seedling.
The present invention is described in further detail below in conjunction with specific embodiment.
Specific embodiment
Embodiment 1
A kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling, comprising the following steps:
1) it takes root the selecting of material: selecting the Chinese catalpa group that plant height is 2~4cm, subculture cycle is 30 days, leaf color is dark green
Seedling is trained, radical leaves, axillary bud are cut off, 3~5 blades being fully deployed are stayed at top, obtain materials A of taking root；
2) it takes root material wound healing: the cutting part for materials A of taking root being coated into wound healing agent for plants, later access training
It supports and is cultivated 3 days in base M, condition of culture are as follows: 25 DEG C of cultivation temperature, intensity of illumination 3000Lux light irradiation time 12h/ days, is given birth to
Root timber material B；Wherein, the culture medium M are as follows: 60 mesh vermiculites and deionized water sterilize at 121 DEG C and be made after 10min, wherein 60
Mesh vermiculite: the volume ratio of deionized water is=1:1.The wound healing agent for plants is the mixture of fungicide and adjuvant, and
The concentration for adding deionized water to dilute 800-1500 times for fungicide using concentration of wound healing agent for plants.The fungicide and auxiliary
The mixability of agent mixture is can make adhesive mixture on the cutting part of plant.Used in the present embodiment
Carbendazim is state's light board, and active constituent content 50%, dosage form is the carbendazim of wettable powder, and its chemical name is N- (2-
Benzimidazolyl)-methyl carbamate.The fungicide is one of carbendazim or potassium permanganate, and the adjuvant is grass
One kind of wood ash or pulverized limestone.
3) tissue-cultured seedling cuttage root-taking: coating nutrient solution N for the notch for material B of taking root, and it is raw that cuttage is carried out in matrix I
Root is cultivated 10 days, condition of culture are as follows: 25 DEG C of air themperature, the soil moisture is 28 DEG C, air humidity >=80%, and illumination condition is
Rooted seedling C is made in 6000Lux；Wherein, the Chinese catalpa cuttage root-taking matrix I includes perlite, turf and the leech that stratiform is laid with
Stone；Wherein, matrix I includes the perlite of lower layer 3cm thickness, the turf of middle layer 3cm thickness and the vermiculite of upper layer 1cm thickness；
4) nutrition pot seedling is fostered: rooted seedling C being transplanted into the nutritive cube equipped with matrix II, is cultivated 25 days, condition of culture
Are as follows: air themperature is 25 DEG C；The soil moisture is 28 DEG C；Preceding 7 days air humidity >=80% after transplanting, after gradually to natural humidity；
Preceding 7 days intensity of illumination≤10000Lux after transplanting, after gradually to natural lighting, cultivate to obtain Chinese catalpa rooted seedling D；Wherein, the matrix
II is bi-layer matrix, and wherein overlayer matrix is the vermiculite of volume ratio 1:1: perlite, and underlying substrate is the grass of volume ratio 1:2:1
Charcoal: vermiculite: perlite, and the volume ratio of overlayer matrix and underlying substrate is 1:2.
The Chinese catalpa rooted seedling as made from the above method can under room temperature natural environment long root, have a large amount of palpuses on main root
Root, root activity is strong, and the nutrition after new root issued in 3-5 days in direct absorption base promotes young leaves pumping, and root survival is high,
It can reach 98%, leaf color is dark green, 25-30 days plant height 6cm or more.
Embodiment 2-6
Embodiment 2-6 is respectively a kind of flat outer rooting method of Chinese catalpa tissue-cultured seedling, and step is similar to Example 1, different
Place is only that the technical parameter being directed to is different, is specifically detailed in following table:
Through the foregoing embodiment Chinese catalpa rooted seedling made from 2-6 method can under room temperature natural environment long root, on main root
There are a large amount of fibrous roots, root activity is strong, and the nutrition after new root issued in 3-5 days in direct absorption base promotes young leaves pumping, and takes root
High survival rate can reach 98% or more, and leaf color is dark green, 25-30 days plant height 6cm or more.
Comparative example
The processing mode used in the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling and wherein used culture medium, to Chinese catalpa
The cultivation results for setting rooted seedling have direct influence.
Using taking root, material wound healing mode is handled, and processing result is as shown in the table:
As can be seen from the above table, when material wound healing time of taking root is 3 days, 100% plant wound healing, part shape
At callus, 3 days after cuttage, survival rate 100%.
The Chinese catalpa rooted seedling cultivation results taken root using matrix I are as follows:
As can be seen from the above table, matrix I with a thickness of 7cm, only with the ratio uniform point of a kind of single matrix or matrix
Timing, will lead to plant the death rate rise, when the use of lower layer is 3cm perlite, middle layer is 2cm turf, upper layer 2cm
The matrix I of vermiculite, plant leaf color is dark green, and 7 days or so young leaves are sprouted, and 14 days whens grow tall 1cm or more, and the plant death rate reduces.
The Chinese catalpa rooted seedling cultivation results taken root using matrix II are as follows:
As can be seen from the above table, the matrix species and ratio used in matrix II are very crucial, and use double-layer base
Matter rots to spend compared with the matrix of simple layer to be reduced, and 2/3 volume of lower layer is turf: vermiculite: perlite=2:1:2 (volume ratio), upper layer
1/3 volume is vermiculite: perlite=1:1 matrix II, plant leaf color is dark green, and 7 days or so young leaves are sprouted, and 14 days whens grow tall 1cm
Or more, the plant death rate reduces.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art is changed or is modified as equivalent variations as enlightenment possibly also with above-mentioned technology contents
Equivalent embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments
Made simple modification, equivalent variations and remodeling, still fall within the protection scope of the claims in the present invention.
Claims (11)
1. a kind of outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling, it is characterised in that it the following steps are included:
1) it takes root the selecting of material: selecting the Chinese catalpa group that plant height is 2~4cm, subculture cycle is 25~35 days, leaf color is dark green
Seedling is trained, radical leaves, axillary bud are cut off, 3~5 blades being fully deployed are stayed at top, obtain materials A of taking root；
2) it takes root material wound healing: the cutting part for materials A of taking root being coated into wound healing agent for plants, accesses culture medium later
It is cultivated 3~5 days in M, obtains material B of taking root；
3) tissue-cultured seedling cuttage root-taking: the notch for material B of taking root is coated into nutrient solution N, cuttage root-taking is carried out in matrix I, is trained
It supports 7~14 days, rooted seedling C is made；Wherein, the Chinese catalpa cuttage root-taking matrix I include stratiform be laid with perlite, turf and
Vermiculite；
4) nutrition pot seedling is fostered: rooted seedling C being transplanted into the nutritive cube equipped with matrix II, Chinese catalpa is obtained within culture 20-28 days and takes root
Seedling D；Wherein, the matrix II is bi-layer matrix, and wherein overlayer matrix is the vermiculite of 1~2:1 of volume ratio: perlite, lower layer's base
Matter is 1~3:1 of volume ratio~2:1 turf: vermiculite: perlite.
2. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: the training in the step 2)
Support base M are as follows: 30-60 mesh vermiculite and deionized water sterilizes obtained after 10min, wherein 30-60 mesh vermiculite at 121 DEG C: deionization
The volume ratio of water is=1:1.
3. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 2, it is characterised in that: the plant wound heeling
Agent is the mixture of fungicide and adjuvant, and the use concentration of wound healing agent for plants is that fungicide adds deionized water to dilute
800-1500 times of concentration.
4. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 3, it is characterised in that: the fungicide is more bacterium
One of spirit or potassium permanganate, the adjuvant are one kind of plant ash or pulverized limestone.
5. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: the training in the step 2)
The condition of supporting are as follows: 24~28 DEG C of cultivation temperature, 3000~5000Lux of intensity of illumination, light irradiation time 10~14h/ days.
6. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: the battalion in the step 3)
Nutrient solution N is mass/volume than the talcum powder for 1.5~4:1: methyl α-naphthyl acetate, and wherein the concentration of methyl α-naphthyl acetate is 0.3~1.0mg/L.
7. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: the training in the step 3)
Support condition are as follows: 22~28 DEG C of air themperature, the soil moisture be 24~30 DEG C, air humidity >=80%, illumination condition be 5000~
7000Lux。
8. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: the step 3) mesostroma
I includes the perlite of 3~5cm thickness, the turf of 1~3cm thickness and the vermiculite of 1~3cm thickness.
9. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 8, it is characterised in that: the step 3) mesostroma
Lower layer is perlite in I, and middle layer is turf, and upper layer is vermiculite.
10. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: in the step 4)
Condition of culture are as follows: air themperature is 22~28 DEG C；The soil moisture is 24~30 DEG C；Preceding 7 days air humidity >=80% after transplanting, after
Gradually to natural humidity；Preceding 7 days intensity of illumination≤10000Lux after transplanting, after gradually to natural lighting.
11. the outside sprout-cultivating-bottle method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that: in the step 4)
The volume ratio of II overlayer matrix of matrix and underlying substrate is 1:2.
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| CN111213585A (en) * | 2020-02-18 | 2020-06-02 | 鲁东大学 | One-step tissue culture and rapid propagation cultivation process for catalpa bungei |
| CN112470938A (en) * | 2021-01-14 | 2021-03-12 | 河南省农业科学院园艺研究所 | Micro-cutting seedling raising and rapid propagation method for catalpa bungei tissue culture seedlings |
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