KR20210001633A - Method for growing Pistacia weinmanniifolia J. Poiss. ex Franch. - Google Patents

Abstract

The present invention relates to a method for cultivating Pistacia weinmannifolia comprising the following steps of: obtaining seedlings by germinating Pistacia weinmannifolia seeds; cultivating the seedlings in a seedling cup filled with cultivation soil; and then harvesting Pistacia weinmannifolia fine roots. By using the method for cultivating Pistacia weinmannifolia provided in the present invention, it is possible to effectively harvest fine roots exhibiting pharmacological activity by limiting the overgrowth of Pistacia weinmannifolia, and thus the Pistacia weinmannifolia fine roots can be widely used in the development of formulations using Pistacia weinmannifolia.

Description

Method for growing Pistacia weinmanniifolia J. Poiss. ex Franch.}

The present invention relates to a method of cultivation of blueberry trees, and more specifically, the present invention is to obtain a seedling by germinating blueberry seeds, and then cultivating the seedlings in a seedling cup filled with cultivation soil, and harvesting the roots of blueberry trees. It relates to a cultivation method of the blueberry tree comprising the steps.

The blue-green tree (Pistacia weinmanniifolia J. Poiss. ex Franch.) is a woody plant of an evergreen subarctic tree native to Yunnan, China, and is an Anacardiaceae plant that grows up to 2 to 8 m in height. Cheonghyangmok has recently been identified for its pharmacological activity.The extract extracted from the roots of Cheonghyangmok has an anti-inflammatory effect, has therapeutic effects on asthma and chronic obstructive pulmonary diseases, as well as anti-aging, skin wrinkles, etc. It has been reported that it exhibits various pharmacologically active effects of (Ministry of Science, ICT and Future Planning, press release on August 3, 2016).

Accordingly, it was attempted to cultivate Cheonghyang trees exhibiting the pharmacologically active effect in large quantities, but due to the nature of woody plants, a lot of cultivation time is required, and it is not easy to establish the climate and soil conditions of Yunnan, the country of origin, and grows more than 2 meters. Various problems have been derived that it is not easy to cultivate a green hyang tree due to the nature of the green hedge tree, but a method of cultivating a green hedge tree by solving this problem has not yet been reported.

Under this background, the present inventors have made diligent research efforts to develop a method for cultivating blueberry more easily, and as a result, developed a method of cultivating blueberry seedlings in a cultivation cup, and completed the present invention.

The main object of the present invention is to provide a method for cultivation of a green hedgehog including the step of germinating a green hedgehog seed to obtain a seedling, cultivating the seedling in a seedling cup filled with cultivated soil, and harvesting the green hedgehog will be.

One embodiment of the present invention for achieving the above object is (a) sowing and cultivating blueberry seeds in cultivated soil, and germinating; (b) cultivating the germinated blueberry seeds to obtain blueberry seedlings; (c) transplanting the obtained seedlings into seedling cups filled with cultivated soil and cultivating them for 2 years; And, (d) harvesting the chunghyang tree seedlings cultivated in the seedling cup, and separating and recovering the tail roots and the main roots from the harvested chunghyang tree seedlings, it provides a cultivation method of the blueberry.

The cultivation soil of the step (a) is not particularly limited as long as it can be used to germinate and grow the seeds of blueberry, but as an example, cultivation containing coco peat, peat moss, perlite, vermiculite, zeolite, trace components, etc. Soil may be used, and as another example, 60 to 80% (w/w) of coco peat, 10 to 20% (w/w) of peat moss, 1 to 10% (w/w) of perlite, 1 to 10 It is possible to use cultivated soil containing% (w/w) vermiculite, 1 to 10% (w/w) zeolite and 1 to 5% (w/w) trace components. At this time, the trace component is also not particularly limited thereto, but may include fertilizers, wetting agents, pH adjusting agents, and the like.

In addition, the cultivated soil may additionally include ground manure to promote the germination of the cultivated tree, and the content of the ground manure is not particularly limited thereto, but as an example, 10 to 30% (w/w) based on the weight of the cultivated soil. ), and as another example, it may be 15 to 25% (w/w) based on the weight of the cultivated soil, and as another example, it may be 20% (w/w) based on the weight of the cultivated soil.

It is preferable to use sterilized and dried seeds before sowing as much as possible.

In order to promote the germination and seedling formation of blueberry seeds during the cultivation of steps (a) and (b), it is preferable to maintain appropriate conditions, as an example, the shading condition of 80 to 95%, 20 to 30 It is possible to maintain the temperature condition and water supply condition of ℃, as another example, the shading condition of 85 to 82%, the temperature condition and the water supply condition of 22 to 27 ℃ can be maintained, as another example, the shading condition of 90%, It can maintain the temperature condition and water supply condition of 25℃.

In addition, the cultivation of step (a) and step (b) is preferably carried out by a field cultivation method or a tray cultivation method in which the cultivated soil is cultivated in the field where the cultivated soil is sprayed with a certain thickness. In the case of field cultivation so that it does not come into direct contact with the general soil, after spreading the cultivation soil on top of the plastic, etc., you can sow and cultivate the blueberry seeds on the sprayed cultivation soil. A square seedling tray is placed in a position where vinyl is laid on the floor, and after cultivating soil is placed inside the tray, the cultivation soil can be sown and cultivated the blueberry seeds.

After sowing the blueberry seeds, the time required to germinate is not particularly limited thereto, but as an example, it may be 10 to 20 days, and as another example, it may be 2 weeks.

In addition, the time required to cultivate the germinated blueberry seedlings to obtain the blueberry seedlings is not particularly limited thereto, but may be 15 to 25 days as an example, and 3 weeks as another example.

In the case of cultivation of blueberry seedlings in the above step (c), it is preferable to water regularly so that the cultivated soil does not dry. As an example, water can be watered at a certain time every day, and as another example, watering for 1 hour every day It can be done, and as another example, it can be watered regularly from 10 am to 11 am every day.

In addition, for effective growth of blueberry seedlings, it can be cultivated while ventilating, but it is preferable to ventilate the cultivated soil to a level that does not dry out due to excessive ventilation.

Since step (c) is cultivated in a seedling cup, after cultivation for a certain period of time, additional fertilizer must be applied. The time to perform the fertilizer fertilization is not particularly limited thereto, but it is preferable to fertilize the fertilizer at the time 3 months, 7 months and 11 months have elapsed after starting the cultivation of step (c).

In addition, the green hedgehog grows up to 2 to 8 m in general, and in particular, in the initial growth of the green hedgehog seedlings, all nutrients absorbed from the fertilizer are consumed for the growth of the stem, so the growth of the root is relatively insufficient, After the stem has grown to a certain level, the root will develop. Accordingly, in order to slow the growth of the stem and to develop the root early, after cultivating the blue-green tree seedlings for a certain period of time, the growth point of the stem was removed to suppress the growth of the stem. At this time, the timing of removing the growth point of the stem developed from the blueberry seedling is not particularly limited thereto, but it is preferable to cut the above-ground part after 7 months have elapsed after starting the cultivation of step (c).

In the cultivation method of the blue scented tree, one thing to be aware of is that the growth of the tail roots among the roots of the sage tree should be promoted. In other words, among the roots of Cheonghyangmok, the pharmacological activity effect is contained at a relatively high level in the tailgum rather than the main root, so it is preferable to promote the growth of the tailgum while cultivating Cheonghyangmok.

To this end, it is desirable to use cultivated soil that can inhibit the formation of the coccyx and promote the formation of the coccyx rather than the general soil that causes the loss of the coccyx formed and to minimize the loss of the formed coccyx. When cultivated in general soil containing soil particles, it is not easy to take root in the soil, and the main root is intensively developed for effective growth of the root. On the other hand, in the case of cultivation in cultivated soil having a certain size and density, it is easy to take root in the cultivated soil, so that not only the main roots are intensively developed, but also the development of the tail roots is promoted.

If the cultivation method of chunghyang tree provided by the present invention is used, since it is possible to effectively harvest tail roots exhibiting pharmacological activity by limiting the overgrowth of chunghyang tree, it will be widely used in the development of a formulation using chunghyang tree.

1 is a schematic diagram showing the standard and spacing of the gyrus.
2 is a photograph of a 105-ball square seedling tray.
3 is a photograph of a seedling cup.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of blueberry seeds

From July to August, red ripe blueberry fruits were collected in the vicinity of Kunming, Yunnan Province. The collected fruit was put in a mesh net, rubbed to remove the pulp, and the seeds were put in 2% Sodium Hypochlorite (Lax) solution and immersed for 20 minutes to sterilize. The sterilized seeds were taken out, put in a basket, dried in the shade, and sown within 2 weeks. The seeds remaining after sowing were sealed and stored in the refrigerator.

Example 2: Preparation of cultivated soil

A mixed cultivated soil was prepared in a ratio (weight ratio) of zeolite 4, pearlite 7, vermiculite 6, coco peat 68, peat moss 14.73, fertilizer 0.201, wetting agent 0.064 and pH adjusting agent 0.005.

Ground manure was added to the prepared cultivation soil, and the basis manure was added as 20% (w/w) of the weight of the cultivated soil.

Example 3: Preparation of furrows and furrows

A ridge with a width of 1.2m and a height of 15cm or more was prepared, and a weeding net was applied on the prepared ridge, and then a weeding vinyl was covered to prevent grass from forming (FIG. 1).

1 is a schematic diagram showing the standard and spacing of the mockup.

In addition, it was covered with weeding vinyl to prevent the roots of blueberry trees from directly contacting the general soil.

Meanwhile, a 40cm furrow was secured to facilitate drainage, movement, and work.

Example 4: Sowing of blueberry seeds

After filling the cultivation soil in the 105-hole square seedling tray, 2-3 seeds of blueberry were sown (Fig. 2).

2 is a photograph of a 105-ball square seedling tray.

At this time, a light-shielding film was installed in the house to shade 90% of light, and then germination and initial growth were performed for 2 weeks. For two weeks, the temperature inside the house was managed so that it did not exceed 30°C, and water was supplied to prevent the cultivation soil from drying out.

Example 5: Management after germination

Cheonghyang tree seedlings cultivated for 3 weeks after germination of Cheonghyang tree seeds were transplanted into seedling cups filled with 90% cultivated soil and cultivated for 2 years. At this time, the seedling cup was used to have a width of 10 to 16 cm and a depth of 10 to 14 cm (Fig. 3).

3 is a photograph of a seedling cup.

During the cultivation, watering was sufficiently performed at 10 to 11 am, and the soil was managed so that it did not dry out, and during the rainy season and winter, the condition of the soil was checked. In addition, the side windows were opened to allow ventilation during the day and the soil was managed so that it did not dry out.

The first fertilization (1/2 of the fertilization amount per area, compound fertilizer) was performed 3 months after sowing (September to October), and the second fertilization (fertilization amount per area, compound fertilizer) was performed 7 months after sowing (February to March). After sowing, 5cm of the above-ground part was cut at 7 months after sowing, and the 3rd fertilization (fertilization per area, compound fertilizer) was performed 11 months after sowing (July to August).

Example 6: Harvesting of blueberry

After removing the second-year seedlings from the seedling cup, the tail roots and the main roots were separated and harvested.

Roughly, the culture soil tangled with the roots and the peat mus are separated, and the first separated roots are immersed in water to remove all the cultivated soil and the peat mus, and then put the washed caudal roots and main roots in a basket to dry the shade for about 7 days, or 65 It was dried for 48 hours in a warmer at ℃. At this time, the moisture content of the dried root was not to exceed 10%.

Example 6: Characteristics of Harvested Blue Hedgehog Roots

The characteristics of the Cheonghyang tree tail roots harvested in Example 5 were analyzed (Table 1).

Characteristics of Harvested Cheonghyang Tree Migeun division 48 cups harvested yield (g) Production per cup (g) Ethyl gallate content (mg/g) Pistachalcon B content (mg/g) Sample 1 (Winter/Winter 14℃ or higher) 74g 1.5g 2.44 2.50 Sample 2 (No greenhouse/winter warming) 24g 0.5g 4.01 1.26

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof, are included in the scope of the present invention.

Claims (15)

(a) seeding and cultivating blueberry seeds in cultivated soil, and germinating;
(b) cultivating the germinated blueberry seeds to obtain blueberry seedlings;
(c) transplanting the obtained seedlings into seedling cups filled with cultivated soil and cultivating them for 2 years; And,
(d) harvesting the cheonggyang tree seedlings grown in the seedling cup, and separating and recovering the tail roots and the main roots from the harvested chunghyang tree seedlings.
The method of claim 1,
The cultivation of step (a) is 60 to 80% (w/w) of coco peat, 10 to 20% (w/w) of peat moss, 1 to 10% (w/w) of perlite, 1 to 10% (w/w) vermiculite, 1 to 10% (w/w) zeolite, and 1 to 5% (w/w) trace components.
The method of claim 2,
The trace component is a fertilizer, a humectant, and a pH adjusting agent containing a cultivation method of blueberry.
The method of claim 1,
The cultivation soil of the step (a) further comprises a ground manure, the cultivation method of blueberry.
The method of claim 4,
The amount of the base manure is 10 to 30% (w / w) relative to the weight of the cultivated soil, the cultivation method of blueberry.
The method of claim 1,
The method of cultivation of the blueberry tree in the step (a), which is sterilized and dried before sowing.
The method of claim 1,
The cultivation of steps (a) and (b) is carried out under a shading condition of 80 to 95%, a temperature condition of 20 to 30°C, and a water supply condition.
The method of claim 1,
The cultivation of steps (a) and (b) is carried out by a field cultivation method or a tray cultivation method.
The method of claim 1,
The cultivation of the step (a) is carried out for 10 to 20 days, the cultivation method of blueberry.
The method of claim 1,
The cultivation of step (b) is to be carried out under the condition that the roots of the blueberry seedlings do not directly contact the general soil.
The method of claim 1,
The cultivation of step (a) is carried out for 15 to 25 days, the cultivation method of the blueberry.
The method of claim 1,
The cultivation of the step (c) is carried out while watering regularly at 10 am to 11 am every day.
The method of claim 1,
The cultivation of the step (c) is performed while ventilating the cultivation soil to a level where it is not dried.
The method of claim 1,
The cultivation method of the blueberry tree is fertilized at the time of 3 months, 7 months and 11 months after starting the cultivation of step (c).
The method of claim 1,
The above-ground part is cut at the time that 7 months have elapsed after starting the cultivation of the step (c), thereby limiting the growth of the blueberry seedlings.

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