CN107852892A - A kind of method for reducing disease, accelerating tobacco seed emergence - Google Patents
A kind of method for reducing disease, accelerating tobacco seed emergence Download PDFInfo
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- CN107852892A CN107852892A CN201711165381.9A CN201711165381A CN107852892A CN 107852892 A CN107852892 A CN 107852892A CN 201711165381 A CN201711165381 A CN 201711165381A CN 107852892 A CN107852892 A CN 107852892A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses one kind to reduce disease, accelerate the method for tobacco seed emergence, including seed disinfection, seed thin layer moisture film vernalization, quantitative program request and heat and moisture preserving determine root four steps of sprouting free of discontinuities, germ entrained by seed is killed by seed disinfection, virus and vernalization amplification seed grain realize quantitative program request, the key technologies such as heat and moisture preserving reach reduction disease, accelerate the purpose that seed is sprouted, it is viral according to entrained by tobacco seed, species of germ etc., to not appreciably affect germination as principle, the method being combined using physics and chemistry kills virus, germ, reduce malicious source.Compared with direct seeding and seedling raising, the present invention has the effect of detoxic, sterilizing simultaneously, particularly detoxification efficiency is obvious, more than 90% is can reach with respect to prevention effect, save seed, the workload of thinning is reduced simultaneously, shortens the heat and moisture preserving time of 7 14 days, is suitable for applying during nursery during tobacco resource/strain/strain material breeding, comparative experiments.
Description
Technical field
The invention belongs to tobacco cultivation technical field, and in particular to a kind of side for reducing disease, accelerating tobacco seed emergence
Method.
Background technology
Tobacco intensive seedling production requires the seeding quality of tobacco seed more and more higher, vernalization coating Pelleting tobacco seed solution
The problem of method for culturing seedlings for having determined traditional wastes seed, thinning is time-consuming, can accomplish one, a cave in production, more conventional
Pelleted seed seedling-growing time is greatly shortened, and emergence rate, seedling quality and neat degree are significantly improved, and resistance against diseases is remarkably reinforced
（Marvin's extensively waits seed primings to apply Zhejiang Agriculture science in China's leaf tobacco production with vernalization coating pill-type technology
.2011(1) : 211-215.）.But the vernalization mode of this scale is not suitable for being applied to the materials such as tobacco resource/strain/strain
The vernalization of seed during material breeding, comparative experiments.The particularly frequency numerous added-generation of breeding intermediate materials, Seed transmission, which carries disease germs, to be compared
Seriously.The seed average band poison rate that existing research shows to harvest on the diseased plant of TMV infection is more than 50%（The such as Yang Song tobaccos are not
Seed-transmission rate after same time inoculation tobacco mosaic virus (TMV) compares Zhejiang Agriculture science, 2005 (5): 409-410.）, cigarette
Grass seed brown spot pathogen bacterial bearing rate is up to 18%, prairie fire germ 8-16%（The tobacco seeds such as Hua Zhifu carry disease germs at analysis and seed
Manage Chinese tobaccos, 1994 (4):40-42.）, the seed with poison during nursery can cause the generation of seedbed virosis（The such as Li Fan are baked
The major transmission path tobaccos science and technology of common mosaic, 2006 (10) in cigarette floating seedlings: 53 -55.）, tobacco seed
Detoxification（The such as Jessica Lynch's English tobacco viruses band seed culture of viruses and its detoxification treatment Fujian tobaccos, 1994 (2):27 -29.）, sterilizing
（Seed disinfection technical research Chinese Tobacco Sciences before the tobacco Aseptic seedling cultures such as Hu Chongyi, 2007 (2): 5-7）Often
Single a certain technology is provided, only inhibits the generation of certain class disease, and polytype can not be taken into account（The disease that i.e. tobacco seed passes
Poison, germ all types）.The vernalization of Seed germination bed, the shaking table vibration vernalization of routine（Chen Ting person of outstanding talent's tobacco seed vernalization new technologies
Research tobaccos science and technology, 1997 (1): 42-43.）Presprouting of seeds is just often stopped into vernalization to showing money or valuables one carries unintentionally, needed during sowing
Hui Gan, then program request is sowed i.e. as naked seed using traditional direct seeding method, can not overcome that application rate is big, thinning work
The problem of measuring.Therefore, the sterilization to a small amount of tobacco seed such as these tobacco resources, breeding intermediate materials, vernalization, seeding technique
Need separately to seek approach, otherwise will have a strong impact on the development of follow-up experiment.
The content of the invention
It is an object of the invention to provide a kind of method for reducing disease, accelerating tobacco seed emergence.
The object of the present invention is achieved like this, including seed disinfection, the vernalization of seed thin layer moisture film, quantitative program request and insulation
Root four steps of sprouting free of discontinuities are determined in moisturizing, specific as follows：
A, seed disinfection：The method being combined using physics and chemistry carries out seed disinfection；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2-3 times, it is placed in culture dish, adds 2-4 mm
Deep thin layer clear water, 25-29 DEG C of temperature, intensity of illumination 3500-3750 Lx, photoperiod are 14 hours dark bars in illumination/10 hour
Vernalization under part, daily cleaning change water 1-2 times, stop vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 5-7 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.
Compared with prior art, the invention has the advantages that：
1st, the germ of the invention first passed through entrained by seed disinfection killing seed, virus, reduce pathogeny, then pass through vernalization and amplify kind
Sub- particle realizes that the key technologies such as quantitative program request, heat and moisture preserving reach and accelerates the purpose that seed is sprouted, and is not influenceing germination
In the case of, disease is effectively reduced, accelerates seed sprouting.
2nd, seed disinfection of the invention mainly for carried in tobacco seed tobacco mosaic virus (TMV), tobacco brown spot pathogen
（Fungi）, tobacco smoke pollution（Bacterium）It is that 45-50 DEG C hot water treatment of seeds is soaked with chemical agent using physics heat treatment etc. cause of disease
Steep the method being combined to carry out, more can thoroughly kill all cause of diseases of seed carrying, the effect with detoxic, sterilizing is special
It is not that detoxification efficiency is obvious, more than 90% is can reach with respect to prevention effect.Cigarette is soaked by 45-50 DEG C of 0.1% silver nitrate solution
Grass seed 5-10 minutes, while kill the cause of diseases such as the virus carried in seed, fungi, bacterium, or first with 10% tertiary sodium phosphate
Solution room temperature impregnated tobacco seed 50-60 minutes kill the virus carried in seed, then are soaked with 45-50 DEG C of 1% copper-bath
Tobacco seed 3-5 minutes are steeped, kill the cause of diseases such as the fungi carried in seed, bacterium.
3rd, the present invention is that can reach sealing, moisturizing and ventilative double effectses using the vernalization of thin layer moisture film, is avoided traditional
Vernalization shows money or valuables one carries unintentionally Hui Gan, finally completes the sprouting of seed incessantly in the environment of dim light, thermophilic, high humidity, shortens 7-14 days
Seed sprout need the heat and moisture preserving time；
4th, the present invention realizes quantitative point compared with the direct seeding and seedling raising of conventional method using presprouting of seeds amplification seed grain
Broadcast, save seed dosage and the workload of thinning；
5th, the present invention is suitable for applying during the nursery stage during tobacco resource/strain/strain material breeding, comparative experiments.
Embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not any limitation as in any way,
Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
The method of the present invention for reducing disease, accelerating tobacco seed emergence, seed is killed by seed disinfection and taken
The germ of band, virus and vernalization amplification seed grain realize the key technologies such as quantitative program request, heat and moisture preserving reach reduce disease,
Accelerate the purpose that seed is sprouted, including to determine root free of discontinuities for seed disinfection, the vernalization of seed thin layer moisture film, quantitative program request and heat and moisture preserving
Four steps are sprouted, it is specific as follows：
A, seed disinfection：The method being combined using physics and chemistry carries out seed disinfection；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2-3 times, it is placed in culture dish, adds 2-4 mm
Deep thin layer clear water, 25-29 DEG C of temperature, intensity of illumination 3500-3750 Lx, photoperiod are 14 hours dark bars in illumination/10 hour
Vernalization under part, daily cleaning change water 1-2 times, stop vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 5-7 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.
The method that physics and chemistry described in step A are combined is to be soaked using 45-50 DEG C of 0.1% silver nitrate solution
Tobacco seed 5-10 minutes.
The method that physics described in step A and chemistry are combined for first with 10% trisodium phosphate solution soaking at room temperature cigarette
Grass seed 50-60 minutes, then with 45-50 DEG C of 1% copper-bath impregnated tobacco seed 3-5 minute.
Matrix described in step C is tobacco floating seedling culture matrix, tobacco seedling nutritious soil, the 0.63-5.00 of commercialization
Any of the mountain sand of mm particle diameters or river sand.
Program request described in step C is each cavities program request 1 ~ 2.
Sheltering from heat or light described in D steps, heat and moisture preserving, low light condition be specifically 20-29 DEG C of temperature, relative humidity 80-90%,
Intensity of illumination 1500-2500 Lx.
The application in described method nursery stage during tobacco resource/strain/strain material breeding, comparative experiments.
Seedlings nursing plate described in step C is floating disc or pallet.
Embodiment 1 --- wild tobacco resource nursery
66 parts of wild tobacco resources（The such as He Bin cigarette category wild species main plant characters and black shank fastness evaluation tropical crops
Journal, 2017,38 (6):1028-1034.）Nursery is compared, using 2 kinds of methods, the first using the present invention method, second
The direct seeding and seedling raising of kind（The direct program request nursery of traditional naked seed）, investigate the emergence situation of two methods, the hair into seedling stage virus
Raw situation.Using comprising the following steps that for the present invention：
A, seed disinfection：First with 10% trisodium phosphate solution soaking at room temperature tobacco seed 50 minutes, then with 45 DEG C of 1% copper sulphate
Solution impregnated tobacco seed 3 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2 times, it is placed in culture dish in ware, adds 2mm
Deep-thin layer clear water, vernalization under 25 DEG C of temperature, the Lx of intensity of illumination 3500,14 hours photoperiods illumination/10 hour dark condition, often
Its cleaning changes water 1 time, has just exposed within 3-5 days green cotyledon and has stopped vernalization；
C, quantitative program request：The dew cotyledon seedling formed to step B adds clear water to dilute, and is dipped with small medicine spoon or 1 children of toothpick picking
Seedling point is sowed in the floating disc cavities for installing matrix；
D, heat and moisture preserving determines root sprouting free of discontinuities：The floating disc of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, it is heat and moisture preserving under the conditions of shelter from heat or light 20 DEG C of transparent plastic Small plastic shed, relative humidity 80%, intensity of illumination 1500Lx, weak
Optical culture 5 days, allows seed sprouting free of discontinuities, radicle is colonized in Medium Culture, cotyledon period seedling is formed, by tobacco intensive seedling production
Technical regulation continues nursery.
Test result indicates that 66 materials to be tested reach 100%, conventional method using the inventive method emergence number
74.24%, average seedling-growing time the inventive method shortens 14 days than tradition, the seedling infection mosaic disease number present invention accounts for 7.58%,
And tradition accounts for 34.85%, the infection seedling average attack rate present invention significantly reduces than tradition, reaches 92.02% with respect to prevention effect
（Table 1）.
The wild tobacco resource nursery results contrast of table 1
Embodiment 2 --- the big gold dollar of safflower and Beinhart1000-1 first backcross generation strain nursery
87 parts of big gold dollars of safflower are compared with Beinhart1000-1 first backcross generation strain nursery, using 2 kinds of methods, the first this hair
Bright method, second of direct seeding and seedling raising（The direct program request nursery of traditional naked seed）, investigate emergence situation, the seedling of two methods
A situation arises for phase virus.Using comprising the following steps that for the present invention：
A, seed disinfection：45 DEG C of 0.1% silver nitrate solution impregnated tobacco seed 5 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 3 times, it is placed in culture dish in ware, adds 4 mm
Deep-thin layer clear water, vernalization under 29 DEG C of temperature, the Lx of intensity of illumination 3750,14 hours photoperiods illumination/10 hour dark condition, often
Its cleaning changes water 2 times, just exposes green cotyledon within 5 days and stops vernalization；
C, quantitative program request：The dew cotyledon seedling formed to step B adds clear water to dilute, and is dipped with small medicine spoon or 2 childrens of toothpick picking
Seedling point is sowed in the pallet cavities for installing matrix；
D, heat and moisture preserving determines root sprouting free of discontinuities：The pallet of step C program request seedling is placed in pond, water depth is no more than cavities
Matrix 1/2, heat and moisture preserving, dim light training under the conditions of 29 DEG C of the transparent plastic greenhouse that shelters from heat or light, relative humidity 90%, the Lx of intensity of illumination 2500
Support 7 days, allow radicle to be colonized in Medium Culture, form cotyledon period seedling, continue nursery by tobacco intensive seedling production technical regulation.
Test result indicates that 87 materials to be tested reach 100%, conventional method using the inventive method emergence number
91.95%, average seedling-growing time the inventive method shortens 9 days than tradition, the seedling infection mosaic disease number present invention account for 6.90% and
Tradition accounts for 40.23%, and the infection seedling average attack rate present invention significantly reduces than tradition, reaches 90.52% with respect to prevention effect（Table
2）.
The big gold dollar of the safflower of table 2 and Beinhart1000-1 first backcross generation strain nursery results contrasts
Embodiment 3
A kind of method for reducing disease, accelerating tobacco seed emergence, it is specific as follows：
A, seed disinfection：Using 50 DEG C of 0.1% silver nitrate solution impregnated tobacco seed 10 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2 times, it is placed in culture dish, adds 3mm depths
Thin layer clear water, 24 DEG C of temperature, the Lx of intensity of illumination 3550, photoperiod are vernalization under 14 hours illumination/10 hour dark conditions, often
Its cleaning changes water 2 times, stops vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk, each cavities program request 1；Described matrix is the tobacco floating seedling culture matrix of commercialization.Described seedlings nursing plate is floating
Disk；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 6 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.It is described shelter from heat or light, heat and moisture preserving, low light condition are specifically 22 DEG C of temperature, relative humidity
82%th, intensity of illumination 1700Lx.Method described in the present embodiment can apply the nursery stage during comparative experiments.
Embodiment 4
A kind of method for reducing disease, accelerating tobacco seed emergence, it is specific as follows：
A, seed disinfection：Using 48 DEG C of 0.1% silver nitrate solution impregnated tobacco seed 8 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2 times, it is placed in culture dish, adds 2.5 mm deep
Thin layer clear water, 27 DEG C of temperature, the Lx of intensity of illumination 3600, photoperiod are vernalization under 14 hours illumination/10 hour dark conditions,
Cleaning changes water 2 times daily, stops vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk, each cavities program request 2；Described matrix is tobacco seedling nutritious soil.Described seedlings nursing plate is pallet；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 5 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.It is described shelter from heat or light, heat and moisture preserving, low light condition are specifically 24 DEG C of temperature, relative humidity
84%th, intensity of illumination 2000Lx.Method described in the present embodiment can apply educating in tobacco lines material breeding experimentation
The seedling stage.
Embodiment 5
A kind of method for reducing disease, accelerating tobacco seed emergence, it is specific as follows：
A, seed disinfection：First with 10% trisodium phosphate solution soaking at room temperature tobacco seed 60 minutes, then with 50 DEG C of 1% copper sulphate
Solution impregnated tobacco seed 5 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 3 times, it is placed in culture dish, adds 3.5 mm deep
Thin layer clear water, 28 DEG C of temperature, the Lx of intensity of illumination 3650, photoperiod are vernalization under 14 hours illumination/10 hour dark conditions,
Cleaning changes water 2 times daily, stops vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk, each cavities program request 2；Described matrix is husky for the mountain of 0.63-0.500mm particle diameters.Described seedlings nursing plate is floating disc；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 5 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.It is described shelter from heat or light, heat and moisture preserving, low light condition are specifically 26 DEG C of temperature, relative humidity
86%th, intensity of illumination 2200Lx.
Embodiment 6
A kind of method for reducing disease, accelerating tobacco seed emergence, it is specific as follows：
A, seed disinfection：First with 10% trisodium phosphate solution soaking at room temperature tobacco seed 55 minutes, then with 48 DEG C of 1% copper sulphate
Solution impregnated tobacco seed 4 minutes；
B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2 times, it is placed in culture dish, adds 4 mm depths
Thin layer clear water, 29 DEG C of temperature, the Lx of intensity of illumination 3700, photoperiod are vernalization under 14 hours illumination/10 hour dark conditions, often
Its cleaning changes water 1 time, stops vernalization when just having exposed green cotyledon within 3-5 days；
C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix
In disk, each cavities program request 1；Described matrix is the river sand of 0.63-5.00 mm particle diameters.Described seedlings nursing plate is floating disc；
D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave
Pore matrix 1/2, shelter from heat or light, cultivated 7 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix
It is interior, form cotyledon period seedling, you can.It is described shelter from heat or light, heat and moisture preserving, low light condition are specifically 28 DEG C of temperature, relative humidity
88%th, the Lx of intensity of illumination 2400.
Claims (8)
- A kind of 1. method for reducing disease, accelerating tobacco seed emergence, it is characterised in that including seed disinfection, seed thin layer moisture film Vernalization, quantitative program request and heat and moisture preserving determine root four steps of sprouting free of discontinuities, specific as follows：A, seed disinfection：The method being combined using physics and chemistry carries out seed disinfection；B, seed thin layer moisture film vernalization：After the seed clear water of step A processing is cleaned 2-3 times, it is placed in culture dish, adds 2-4 mm Deep thin layer clear water, 25-29 DEG C of temperature, intensity of illumination 3500-3750 Lx, photoperiod are 14 hours dark bars in illumination/10 hour Vernalization under part, daily cleaning change water 1-2 times, stop vernalization when just having exposed green cotyledon within 3-5 days；C, quantitative program request：In the dew cotyledon seedling formed to step B plus clear water dilutes, and takes seedling point to be sowed at the nursery for installing matrix In disk；D, heat and moisture preserving determines root sprouting free of discontinuities：The seedlings nursing plate of step C program request seedling is placed in pond, water depth is no more than cave Pore matrix 1/2, shelter from heat or light, cultivated 5-7 days under heat and moisture preserving, low light condition, allow seed sprouting free of discontinuities, radicle is colonized in matrix It is interior, form cotyledon period seedling, you can.
- 2. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in step A The method that the physics and chemistry stated are combined is the 0.1% silver nitrate solution impregnated tobacco seed 5-10 minutes using 45-50 DEG C.
- 3. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in step A The method that the physics stated and chemistry are combined for first with 10% trisodium phosphate solution soaking at room temperature tobacco seed 50-60 minutes, then With 45-50 DEG C of 1% copper-bath impregnated tobacco seed 3-5 minute.
- 4. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in step C The matrix stated is the tobacco floating seedling culture matrix of commercialization, the mountain of tobacco seedling nutritious soil, 0.63-5.00 mm particle diameters is husky or river Any of sand.
- 5. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in step C The program request stated is each cavities program request 1 ~ 2.
- 6. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in D steps State shelter from heat or light, heat and moisture preserving, low light condition are specifically 20-29 DEG C of temperature, relative humidity 80-90%, intensity of illumination 1500-2500 Lx。
- 7. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that described method The application in nursery stage during tobacco resource/strain/strain material breeding, comparative experiments.
- 8. the method according to claim 1 for reducing disease, accelerating tobacco seed emergence, it is characterised in that institute in step C The seedlings nursing plate stated is floating disc or pallet.
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| CN112166989A (en) * | 2020-11-03 | 2021-01-05 | 长沙稻银农业科技有限公司 | Tobacco sowing and seedling raising method |
| CN114051787A (en) * | 2021-11-15 | 2022-02-18 | 玉溪中烟种子有限责任公司 | Tobacco seed bleaching and sterilizing method |
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