CN102379315A - Directional regulating agent of rhizosphere microorganism and nematode containing saturated fatty acid - Google Patents
Directional regulating agent of rhizosphere microorganism and nematode containing saturated fatty acid Download PDFInfo
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- CN102379315A CN102379315A CN2010102730335A CN201010273033A CN102379315A CN 102379315 A CN102379315 A CN 102379315A CN 2010102730335 A CN2010102730335 A CN 2010102730335A CN 201010273033 A CN201010273033 A CN 201010273033A CN 102379315 A CN102379315 A CN 102379315A
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Abstract
The invention relates to a directional regulating agent of rhizosphere microorganisms and nematodes containing saturated fatty acids. The description describes the effect of natural active substance saturated fatty acids (caprylic acid and capric acid) on controlling soil harmful pathogenic microorganisms, and describes the effect of mixtures with different ratios of caprylic acid and capric acid on modifying soil with biological disorders. Meanwhile, the description describes the control effect of plant source material mixtures (only including castor plant residual bodies and rapeseed cakes) on plant pathogenic nematodes - root-knot nematodes. Finally, the the description describes the modification of combined saturated fatty acids (caprylic acid and capric acid) and plant source materials (castor plant residual bodies and rapeseed cakes) to soil biological disorders. The directional regulating agent of rhizosphere microorganism and nematode containing saturated fatty acids are used to treat soil, which can reduce the effect of soil biological disorders on plant growth. The invention has low production cost, significant effect, and is easy to be popularized.
Description
Technical field
The present invention relates to a kind of a kind of rhizospheric microorganism of saturated fatty acid and method that the directed adjusting control agent of nematode is effectively controlled the soil biology obstacle of containing of forming by saturated fatty acid (sad and capric acid) and botanical material (castor-oil plant plant residue and colza cake).
Background technology
Vegetables industry is one of main pillar industry of China's agricultural.According to Ministry of Agriculture's farming feelings statistics, 2.73 hundred million mu of the sowing of vegetable areas in the whole nation in 2009 have increased by 4,940,000 mu on year-on-year basis; 6.02 hundred million tons of gross yields increase by 2,684 ten thousand tons on year-on-year basis; More than 440 kilogram of occupancy volume per person exceeds more than 200 kilogram of world average level.By the end of the year 2008, China's facilities vegetable gross output value reaches 4,100 hundred million yuan, accounts for 51% of the vegetables gross output value.
But; Along with the increase of facilities vegetable ground area with utilize the increase of frequency; Same plot continuously cultivation with a kind of or sibling species plant, cause second batch after the quality and yield of plant obviously reduce, it is also more and more serious that the frequent phenomenon that obviously increases takes place damage by disease and insect; This succession crop damage by disease and insect is obviously serious, and the phenomenon that the quality and yield of product obviously descends is called continuous cropping obstacle.Under the facility cultivation condition; Crop grows under sealing or semiclosed condition; The field management measure performance difficulty causes crop disease and insect thus and is often taken place by the physiological disturbance that nutrient imbalance causes, the continuous cropping condition has then further increased the weight of the generation of disease; Soil quality also descends gradually, even causes the greenhouse to fall into disuse.Owing to cost of investment is high, land area capable of using is limited and reasons such as cultivation custom and technical ability; The continuous cropping obstacle problem of planting vegetable under the facility cultivation condition (being mainly cucumber, eggplant and tomato) is more and more serious; Statistics shows that there is continuous cropping obstacle in various degree in 20～40% facility cultivation; The annual economic loss that therefore causes is huge, and problem is very outstanding, has become China and the primary problem that solves of city's vegetables sustainable development.
In the soil biology obstacle that the vegetables soil of plantation occurs continuously; The disease fungus that detrimental soil is propagated, the harm of plant pathogeny line insect are one of the main reasons; Seedling is had a low resistance, and the harm that receives is more obvious, how to control the disease fungus of detrimental soil propagation, the harm of plant pathogeny line insect targetedly; Especially take measures to cultivate healthy seedling and improve the seedling resistance, alleviating the soil biology obstacle that vegetables soil occurs greatly.
Summary of the invention
The present invention relates to a kind of rhizospheric microorganism and directed adjusting control agent of nematode that contains saturated fatty acid.The natural active matter saturated fatty acid that the present invention contains (sad and capric acid) has the characteristic of the harmful causal organism effect of control soil, and botanical material (castor-oil plant plant residue and colza cake) is to the common plant pathogeny line insect in greenhouse--the characteristic that root-knot nematode can effectively prevent and treat.The present invention is with saturated fatty acid (sad and capric acid) and the two mixing of botanical material (castor-oil plant plant residue and colza cake); Mix once more with seedling medium; Be applied in the seedling incubation, can improve the resistance of seedling, reduce the harm of disease fungus and root-knot nematode seedling.Production cost of the present invention is low, and effect is remarkable, promotes easily.
The present invention includes selected sad and capric acid is chemical pure.Described botanical material castor-oil plant plant residue comprises castor-oil plant ground and under ground portion, and colza cake is conventional colza cake.
The present invention contains the rhizospheric microorganism of saturated fatty acid and the exploration of the directed adjusting control agent of nematode specifically may further comprise the steps:
The disease resisting effect of A. sad and capric acid:
Take by weighing an amount of fatty acid and be dissolved in the 10ml acetone, the acetone soln of getting 1ml fatty acid joins in the 100ml PDA medium (40 ℃～50 ℃), its ultimate density is respectively: 0,100,1000,2000 μ mol/L.
In the medium for preparing concentration, add 1 ‰ polysorbas20s and make fatty acid abundant mixing in medium, contrast only contains 1 ‰ polysorbas20 and 1% acetone.Tomato is epidemic disease early; Cucumber is withered to wither growth after 8 days with tomato, and the growth of cucumber anthrax used the metal card punch to get the bacterium cake of diameter as 5mm at colony edge after 14 days; Again the bacterium cake is inoculated into the plate central authorities of the medium that contains variable concentrations; 25 ℃ of dark down cultivations, each processing has 6 repetitions (repeat is a culture dish at every turn, wherein inoculates a bacterium cake).
Because the speed of different conks is different, we measure the diameter (deducting the diameter of central bacterium cake) of bacterium colony in different periods.Inoculate the colony diameter of measuring the cucumber anthrax after 12 days, measure the colony diameter of other three kinds of disease funguses after 6 days.Measure colony diameter two vertical direction, get its mean value and calculate inhibiting rate, formula is following:
The diameter % of bacterium colony on percentage mycelial inhibition (%)=100 * (on the control medium on the diameter of bacterium colony-processing medium the diameter of bacterium colony)/control medium
The result shows, sad when concentration is higher mycelia to four kinds of pathogens all show stronger inhibitory action, when its concentration is 1000 μ mol/L, just show to cucumber withered with the withered mycelia significant inhibitory effect of tomato; Be respectively 27% to cucumber is withered with the withered inhibiting rate of tomato; 16%, when sad concentration is elevated to 2000 μ mol/L, selected four plant species disease funguses all there is remarkable inhibitory action; Sad inhibiting rate to tomato epidemic disease morning is 36%; Inhibiting rate to the cucumber anthrax is 100%, and the inhibiting rate withered to cucumber is 32%, and the inhibiting rate withered to tomato is 27% (table 1).It is thus clear that in this test, the mycelia of cucumber anthrax can suppress mycelial growth to sad very responsive when sad concentration is 2000 μ mol/L fully, on this medium, the cucumber anthrax does not form bacterium colony.
Capric acid all shows the mycelial growth significant inhibitory effect four kinds of disease funguses; When low concentration gradient 100 μ mol/L; Capric acid just shows the remarkable inhibitory action to tomato epidemic disease morning and cucumber anthrax mycelia; Inhibiting rate is respectively 27% and 46%, and the cucumber anthrax on the medium of this concentration shows certain sporulation quantity, explains under this concentration and the cucumber anthrax is caused certain effect of coercing.When capric acid concentration was elevated to 1000 μ mol/L, the strongest to the inhibitory action of cucumber anthrax mycelia, inhibiting rate reached 100%; Do not form bacterium colony on the medium; And to the tomato also enhancing to some extent of inhibitory action of epidemic disease early, inhibiting rate is elevated to 46%, when concentration is elevated to 2000 μ mol/L; Inhibiting rate to cucumber anthrax mycelia still is 100%, to tomato early the inhibiting rate of epidemic disease reach 64% (table 1).
B: sad and capric acid mixes the improving effect that uses the soil organisms obstacle.Sad and capric acid mixed proportion was respectively 0: 100, and 25: 100,50: 50,75: 25,100: 0.Preliminary seedbed result of the test shows, have use on the soil of biological barrier after, sad and mixture effect capric acid are better than the application effect of single sad or capric acid.Relevant further test is carried out.
C: the castor-oil plant plant residue mixes the depression effect of using the pathogenic soil nematode with colza cake.Castor-oil plant plant residue and colza cake mixed proportion 0: 100,25: 100,50: 50,75: 25,100: 0.Pot experiment shows that thermal treatment soil inoculation root-knot nematode is after latter two month, and the reproduction coefficient of root-knot nematode is 0 behind the plantation castor-oil plant, and the reproduction coefficient of root-knot nematode is 300 behind the plantation capsicum, and castor-oil plant has the potentiality that the good restraining root-knot nematode grows.Preliminary seedbed result of the test shows that behind the inoculation nematode, castor-oil plant plant residue and colza cake mixture are better than the effect of using castor-oil plant plant residue or colza cake separately to the control efficiency of pathogenic nematode.Relevant further test is carried out.
D: unsaturated fatty acid (sad and capric acid) and botanical material (castor-oil plant plant residue and colza cake mixture) compounding application is to there being the improving effect of biological barrier soil.The soil of soil biology obstacle select to take place, under 65 degrees celsius baking oven thermal treatment 8 hours to kill nematode.With the soil dress basin of handling and " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid " the even dress basin that is mixed, and sowing and and inoculation pathogenic nematode.Wherein the final content (mass percent) of saturated fatty acid (sad and capric acid) in soil matrix is 0.01%-10.0%, and the final content of botanical material (castor-oil plant plant residue and colza cake) in cultivation matrix is 0.01%-10.0% castor-oil plant plant residue.PRELIMINARY RESULTS is indicated; Compare with the control treatment of not adding " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid "; Add the processing of " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid ", plant grew well, strong sprout, index improved.Concrete test effect is also verified.
Embodiment:
Embodiment 1. is sad to mix the improving effect that uses the soil organisms obstacle with capric acid.Sad and capric acid mixed proportion was respectively 0: 100, and 25: 100,50: 50,75: 25,100: 0.Preliminary seedbed result of the test shows, have use on the soil of biological barrier after, sad and mixture effect capric acid are better than the application effect of single sad or capric acid.Relevant further test is carried out.
Embodiment 2: the castor-oil plant plant residue mixes the depression effect of using the pathogenic soil nematode with colza cake.Castor-oil plant plant residue and colza cake mixed proportion 0: 100,25: 75,50: 50,75: 25,100: 0.Pot experiment shows that thermal treatment soil inoculation root-knot nematode is after latter two month, and the reproduction coefficient of root-knot nematode is 0 behind the plantation castor-oil plant, and the reproduction coefficient of root-knot nematode is 300 behind the plantation capsicum, and castor-oil plant has the potentiality that the good restraining root-knot nematode grows.Preliminary seedbed result of the test shows that behind the inoculation nematode, castor-oil plant plant residue and colza cake mixture are better than the effect of using castor-oil plant plant residue or colza cake separately to the control efficiency of pathogenic nematode.Relevant further test is carried out.
Embodiment 3: unsaturated fatty acid (sad and capric acid) and botanical material (castor-oil plant plant residue and colza cake mixture) compounding application is to there being the improving effect of biological barrier soil.The soil of soil biology obstacle select to take place, under 65 degrees celsius baking oven thermal treatment 8 hours to kill nematode.With the soil dress basin of handling and " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid " the even dress basin that is mixed, and sowing and and inoculation pathogenic nematode.Wherein the final content (mass percent) of saturated fatty acid (sad and capric acid) in soil matrix is 0.01%-10.0%, and the final content of botanical material (castor-oil plant plant residue and colza cake) in cultivation matrix is 0.01%-10.0% castor-oil plant plant residue.PRELIMINARY RESULTS is indicated; Compare with the control treatment of not adding " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid "; Add the processing of " rhizospheric microorganism and the directed adjusting control agent of nematode that contain saturated fatty acid ", plant grew well, strong sprout, index improved.Concrete test effect is also verified.
Table 1 sad with capric acid to the inhibitory action of plant pathogenic fungi mycelial growth
Claims (7)
1. rhizospheric microorganism that contains saturated fatty acid and the directed adjusting control agent of nematode is characterized by to the regulation and control of rhizospheric microorganism with to the control of root-knot nematode.
2. according to described a kind of rhizospheric microorganism that contains saturated fatty acid of claim 1 and the directed adjusting control agent of nematode, it is characterized in that the material of directed regulating and controlling microbial in a kind of rhizospheric microorganism that contains saturated fatty acid and the nematode orientation adjusting control agent is selected from C4-C18 fatty acid.
3. according to described rhizospheric microorganism that contains saturated fatty acid of claim 1 and the directed adjusting control agent of nematode, the vegetable material that it is characterized in that preventing and treating root-knot nematode is castor-oil plant plant residue and colza cake, and the two mass ratio is 1: 99 to 99: 1.
4. according to the described C4-C18 saturated fatty acid of claim 2, it is characterized in that containing the rhizospheric microorganism and the directed adjusting control agent of nematode of saturated fatty acid, sad and ratio capric acid is 1: 99 to 99: 1.
5. according to described rhizospheric microorganism that contains saturated fatty acid of claim 1 and the directed adjusting control agent of nematode, saturated fatty acid and botanical material (castor-oil plant plant residue and colza cake) mass ratio are 1: 99 to 99: 1 in the rhizospheric microorganism that it is characterized in that containing saturated fatty acid and the nematode orientation adjusting control agent.
6. according to described rhizospheric microorganism and the directed adjusting control agent of nematode that contains saturated fatty acid of claim 1; Its characteristic comprises the steps: to mix with seedling medium according to the ratio in the claim 5; The final content (mass percent) of saturated fatty acid (sad and capric acid) in soil matrix is 0.01%-10.0%, and the final content of botanical material (castor-oil plant plant residue and colza cake) in cultivation matrix is 0.01%-10.0% castor-oil plant plant residue.
7. according to described a kind of rhizospheric microorganism and the directed adjusting control agent of nematode that contains saturated fatty acid of claim 1, it is characterized in that being used to regulate the soil biology obstacle.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410075A (en) * | 2015-12-28 | 2016-03-23 | 北京市农林科学院 | Botanical reagent for prevention and treatment of meloidogyne and preparation and use method thereof |
| CN112369416A (en) * | 2019-07-04 | 2021-02-19 | 南京农业大学 | Soil microorganism prebiotic composition for increasing plant system resistance and application thereof |
| CN115997766A (en) * | 2022-11-21 | 2023-04-25 | 云南农业大学 | Mixtures of phthalic acid, palmitic acid and azelaic acid and their use |
-
2010
- 2010-09-06 CN CN2010102730335A patent/CN102379315A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410075A (en) * | 2015-12-28 | 2016-03-23 | 北京市农林科学院 | Botanical reagent for prevention and treatment of meloidogyne and preparation and use method thereof |
| CN112369416A (en) * | 2019-07-04 | 2021-02-19 | 南京农业大学 | Soil microorganism prebiotic composition for increasing plant system resistance and application thereof |
| CN112369416B (en) * | 2019-07-04 | 2021-08-17 | 南京农业大学 | Soil microorganism prebiotic composition for increasing plant system resistance and application thereof |
| CN115997766A (en) * | 2022-11-21 | 2023-04-25 | 云南农业大学 | Mixtures of phthalic acid, palmitic acid and azelaic acid and their use |
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Application publication date: 20120321 |