CN105494447A - Biological seed coating agent for controlling soybean root disease and preparation method and application thereof - Google Patents
Biological seed coating agent for controlling soybean root disease and preparation method and application thereof Download PDFInfo
- Publication number
- CN105494447A CN105494447A CN201511009463.5A CN201511009463A CN105494447A CN 105494447 A CN105494447 A CN 105494447A CN 201511009463 A CN201511009463 A CN 201511009463A CN 105494447 A CN105494447 A CN 105494447A
- Authority
- CN
- China
- Prior art keywords
- seed coating
- coating agent
- biological seed
- soybean root
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a biological seed coating agent for controlling a soybean root disease. The biological seed coating agent is prepared from the following components in percentage by mass: 0.02-0.15 percent of CuSO4, 0.05-0.26 percent of (NH4)6Mo7O24.4H2O, 0.01-0.05 percent of H3BO3, 0.1-0.5 percent of MnSO4, 0.04-0.25 percent of FeSO4, 1.5-6 percent of KH2PO4, 0.02-1 percent of K2SO4, 2-8 percent of dispersing agent, 2-5 percent of film-forming agent, 0.1-1.2 percent of warning color, 0.95-2.65 percent of binder and the balance of biocontrol strain fermentation solution. The biological seed coating agent provided by the invention is used for coating soybean seeds, and can achieve the effects of restraining soybean cyst nematode and soybean root rot, so that the soybean root disease is prevented and controlled effectively, soybean plant growth can be promoted, and the yield is increased.
Description
Technical field
The invention belongs to microbial pesticide technical field, be specifically related to a kind of biological seed coating agent controlling soybean cyst nematode Heterodera glycines and root rot, and its preparation method and application.
Background technology
Soybean cyst nematode is by soybean cyst nematode Heterodera glycines (SoybeanCystNematode, SCN) infect and to cause, one of important disease that harm world soybean is produced, soybean cyst nematode all can occur soybean whole breeding time, mainly to cause harm host's root system, be a kind of soil-borne disease of extremely difficult control, often cause heavy losses to Soybean production.This disease generally makes the soybean underproduction 10% ~ 30%, and serious plot can reach 70% ~ 90%, even causes total crop failure, and the harm caused than the single disease of any one is all large.At present, the generation of this disease was all reported in nearly all soybean producing region.In China, (autonomous region, municipality directly under the Central Government) is economized in Heilungkiang, Jilin, Liaoning, the Inner Mongol, Hebei, Henan, Shandong, Shanxi, Shaanxi, Anhui, Hubei, Jiangsu, Beijing, Shanghai etc. all has generation.From world wide, the harm of soybean cyst nematode and spread the trend having and day by day increase the weight of, the research about soybean cyst nematode Heterodera glycines is more and more subject to the attention of various countries.
Root rot be a kind of distributed more widely, harm is serious, Species of Pathogens is various and the important soil-borne disease that control is more difficult, is considered to the principal element of restriction soybean yields in global range.The impact that the generation of this disease can make that the root of soybean plant strain is heavy, root nodule is heavy, nitrogenase activity and side radical are all subject in various degree, and soybean quality is obviously declined.Root rot causes the soybean underproduction throughout the year according to investigations, and the general underproduction 10% ~ 30%, can reach 60% time serious, even have no harvest.In recent years along with the continuous increase of soybean area, meeting next batch of soybean situation is increasingly sharpened, and the harm of root rot is increased the weight of year by year, also increasing on the impact of output, and the economic loss that country causes because of root rot every year reaches several hundred million unit.
But lack the measure effectively controlling soybean cyst nematode evil and soybean root rot disease at present.Although efficent rotation is a kind of Main Agricultural prophylactico-therapeutic measures, the time needed is longer, just can reach dynamic equilibrium after general 12 years, therefore, production is difficult to realize; Although chemical agent can the generation of cost-effective control disease, there is serious " 3R " problem.
What the major soybean production areas control seedling stage diseases and pests use of current China was more is seed coat agent, main insecticidal constituent is Furadan, but this medicine is disabled, therefore the control of damage by disease and insect is turned to biological control by people, find and develop effective, environment amenable pharmaceutical preparations and become inevitable, seed coat agent is made up of active component and non-active ingredient usually, and active component comprises insecticide, bactericide, plant growth regulator, fertilizer, nutritive element, beneficial microbe and product and biopesticide etc.; Non-active ingredient refers to film forming agent in seed coat agent and corresponding auxiliary aid, is the physics and the chemical property that maintain seed coat agent, controls the important component of active component slowly-releasing in clothing film.Biological seed coating agent action principle mainly promotes the fertility of crop surrounding environment, manufactures nutrition and assists plant absorption nutrition, improving the nutritional capacity of plant self simultaneously.In this environment, beneficial microbe and metabolite thereof can be survived and be kept its original activity, are reached the object of prevention and elimination of disease and pests by antagonism and Competition.
Current domestic biological seed coating agent variety protection is single, large-scale production is backward in technique, although to develop, register many biopesticide kinds, the level of production of many kinds is low, preventive effect usefulness is low, does not still reach and optimize accurate production control in microbial pesticide fermentation kind; On aft-loaded airfoil and medicament development, fail to prevent and treat around the soybean cyst nematode of soybean root disease and root rot well, thus promote plant strain growth better.
Summary of the invention
The object of the invention is to obtain a kind of biological seed coating agent can preventing and treating soybean root Major Diseases, the bacterial strain routinely after liquid fermentation and culture killing line activity will be had, with zymotic fluid and the mixture process by a certain percentage such as other chemical element, auxiliary agent etc. of strains tested, form biological seed coating agent, in order to dressing soya seeds, reach and effectively control soybean cyst nematode and root rot, promote Soybean Seedlings Growth, improve the object of output.
For this reason, the invention provides a kind of biological seed coating agent for controlling soybean root disease, comprising following component, each component is prepared by mass percentage,
The CuSO of 0.02% ~ 0.15%
4, (the NH of 0.05% ~ 0.26%
4)
6mo
7o
244H
2o, the H of 0.01% ~ 0.05%
3bO
3, the MnSO of 0.1% ~ 0.5%
4, the FeSO of 0.04% ~ 0.25%
4, the KH of 1.5% ~ 6%
2pO
4, the K of 0.02% ~ 1.0%
2sO
4, the dispersant of 2% ~ 8%, the film forming agent of 2% ~ 5%, the warning colouration of 0.1% ~ 1.2%, the binding agent of 0.95% ~ 2.65%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity.
Preferably, biological seed coating agent provided by the invention comprises following component, and each component is prepared by mass percentage: the CuSO of 0.04%
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4, the K of 0.05%
2sO
4, the dispersant of 6%, the film forming agent of 4.0%, the warning colouration of 1%, the binding agent of 2.55%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity.
Preferably, described biocontrol bacterial strain zymotic fluid is simple fermentation of bacillus liquid.
More preferably, described simple bacillus is separated and obtains from soybean root system soil.
Preferably, described dispersant is calcium lignosulfonate; Described film forming agent is methylcellulose; Described warning colouration is peacock green; Described binding agent is mass percent is the xanthans of 1.5-20: 100 and the mixture of modified starch composition.
Preferably, described biocontrol bacterial strain zymotic fluid is by bacterial strain to be seeded after test tube strains is cultivated, then obtain through expansion fermented and cultured, concrete preparation method is as follows:
Percentage by weight is respectively the beef extract of 0.2% ~ 0.4%, the peptone of 0.4% ~ 0.6%, the yeast extract of 0.1% ~ 0.2%, 0.8% ~ 1% sucrose, the agar of 1.2% ~ 1.8%, all the other are distilled water, regulate pH to be 7 after mixing, bevel medium, simple Bacillus strain to be seeded is inoculated on described slant medium, be placed in 25 DEG C of constant temperature culture 2 days, obtain test tube strains;
Percentage by weight is respectively the beef extract of 0.2% ~ 0.4%, the peptone of 1.0% ~ 1.2%, the yeast extract of 0.5% ~ 1%, 1%-1.5% sucrose and excess water mixing, regulates pH to make liquid fermentation medium after 6.8 ~ 7 afterwards;
Described test tube strains is inoculated in described liquid fermentation medium, at 25 DEG C ~ 28 DEG C, with rotating speed 150r.min
-1~ 200r.min
-1shaken cultivation 24h ~ 72h in shaking table, obtains described biocontrol bacterial strain zymotic fluid.
Present invention also offers a kind of preparation method of the biological seed coating agent for controlling soybean root disease, being specially, percentage by weight is respectively the CuSO of 0.02% ~ 0.15%
4, (the NH of 0.05% ~ 0.26%
4)
6mo
7o
244H
2o, the H of 0.01% ~ 0.05%
3bO
3, the MnSO of 0.1% ~ 0.5%
4, the FeSO of 0.04% ~ 0.25%
4, the KH of 4% ~ 6%
2pO
4with 0.02% ~ 0.08% K
2sO
4grinding is pulverized after mixing, add the dispersant of 2% ~ 8% again, the film forming agent of 2% ~ 5%, the warning colouration of 0.1% ~ 1.2%, the binding agent of 0.95% ~ 2.65%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity, and said components stirs into uniform viscous fluid, vibration mixes, and prepares described biological seed coating agent.
Preferably, be the CuSO of 0.04% by quality accounting
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4with 0.05% K
2sO
4pulverize grinding after mixing, then add the dispersant of 6%, the film forming agent of 4%, the warning colouration of 1%, the binding agent of 2.55%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity, said components stirs into uniform viscous fluid, and vibration mixes, and prepares described biological seed coating agent.
The biological seed coating agent that preparation method of the present invention obtains is for soya seeds coated to be sowed, during use, by mass percent be 1-5: 70 biological seed coating agent mix with soya seeds, make biological seed coating agent evenly be coated on soya seeds surface, can sow after drying.
Because simple bacillus is to the Biocontrol Effect of soybean cyst nematode Heterodera glycines and root rot, biological seed coating agent provided by the invention utilizes from soybean nodulation, is separated the simple bacillus (Sneb545) obtained, zymotic fluid after fermented and cultured and other mixtures such as chemical element, auxiliary agent, make seed coat agent, dressing use is carried out to soya seeds, the effect simultaneously suppressing soybean cyst nematode and root rot can be reached, thus effectively prevent and treat soybean root disease, and can promote that soybean plant strain grows, improve output.
Embodiment
Understand technical scheme of the present invention better to make those skilled in the art can be implemented, below in conjunction with specific embodiment, the invention will be further described, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1
For controlling a biological seed coating agent for soybean root disease, comprise percentage by weight be 90.4% there is the simple fermentation of bacillus liquid killing line activity; And percentage by weight is respectively the CuSO of 0.09%
4, (the NH of 0.18%
4)
6mo
7o
244H
2o, the H of 0.04%
3bO
3, the MnSO of 0.3%
4, the FeSO of 0.04%
4, the KH of 1.5%
2pO
4, the K of 1%
2sO
4, the calcium lignosulfonate of 3%, the methylcellulose of 2%, the peacock green of 0.5%, and by 0.15% xanthans and the binding agent that forms of 0.8% modified starch, above-mentioned each component is prepared by mass percentage.
Concrete preparation process is: by the CuSO of above-mentioned 0.09%
4, (the NH of 0.18%
4)
6mo
7o
24.4H
2o, the H of 0.04%
3bO
3, the MnSO of 0.3%
4, the FeSO of 0.04%
4, the KH of 1.5%
2pO
4, the K of 1%
2sO
4grinding is pulverized after mixing, add the calcium lignosulfonate of 3% again, the methylcellulose of 2%, the peacock green of 0.5%, and by 0.15% xanthans and the binding agent that forms of 0.8% modified starch, and the described simple fermentation of bacillus liquid of 90.4% stirs into uniform viscous fluid, vibration mixes, and prepares described biological seed coating agent.Obtained biological seed coating agent is 1: 70 with the ratio that mixes of grain weight, is evenly wrapped in soya seeds surface, can sows after drying.
Wherein, above-mentioned to have the simple fermentation of bacillus liquid killing line activity be by bacterial strain to be seeded after test tube strains is cultivated, more obtained through expanding fermented and cultured, and concrete preparation method is as follows:
Percentage by weight is respectively the beef extract of 0.3%, the peptone of 0.5%, the yeast extract of 0.1%, 1% sucrose, the agar of 1.5%, all the other are distilled water, regulate pH to be 7 after mixing, bevel medium, simple Bacillus strain to be seeded is inoculated on described slant medium, be placed in 25 DEG C of constant temperature culture 2 days, obtain simple bacillus test tube strains.
Percentage by weight is respectively the beef extract of 0.2%, the peptone of 1%, the yeast extract of 0.5%, 1.0% sucrose, all the other are distilled water again, and said components fully mixes rear adjustment pH and makes simple fermentation of bacillus medium after 6.8.
Described simple bacillus test tube strains is inoculated in liquid simple fermentation of bacillus medium, at 28 DEG C, with rotating speed 150rmin
-1shaken cultivation 48h in shaking table, obtains simple fermentation of bacillus liquid.
Above-mentioned chemical reagent is all the analytical reagent commercially bought, simple bacillus (Bacillussimplex) sneb545 used in the present invention is separated and obtains from soybean root system soil, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 11st, 2012, preserving number is CGMCCNo.6650.
Concrete, above-mentioned simple fermentation of bacillus liquid can carry out large-scale production by large fermentation tank, is fermented by the fermentation tank that simple bacillus is inoculated into 50L ~ 100L, and cultivate at 25 DEG C, initial pH8.0, fermentation time is 48h.Survey sample the activity of zymotic fluid to target nematode between yeast phase, determine to put the tank time according to activity criteria's curve.
Embodiment 2
For controlling a biological seed coating agent for soybean root disease, comprise 80.84% there is the simple fermentation of bacillus liquid killing line activity, the wherein CuSO of 0.04%
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4, the K of 0.05%
2sO
4, the calcium lignosulfonate of 6.0%, the methylcellulose of 4%, the peacock green of 1%, and by 0.05% xanthans and the binding agent that is mixed to form of 2.5% modified starch, above-mentioned each component is prepared by mass percentage.
Concrete preparation process is: by the CuSO of above-mentioned 0.04%
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4, the K of 0.05%
2sO
4grinding is pulverized after mixing, add the calcium lignosulfonate of 6% again, the methylcellulose of 4%, the peacock green of 1.0%, and by 0.05% xanthans and the binding agent that forms of 2.5% modified starch, and the fermentation broth agitation uniformly viscous fluid of the described simple Bacillus strain of 80.84%, vibration mixes, and prepares described biological seed coating agent.Obtained biological seed coating agent and the ratio of grain weight are 3: 70, are evenly wrapped in soya seeds surface, can sow after drying.
Wherein, above-mentioned to have the bacterial strain fermentation liquor killing line activity be simple fermentation of bacillus liquid, and concrete preparation method is as follows:
Percentage by weight is respectively the beef extract of 0.2%, the peptone of 0.4%, the yeast extract of 0.2%, 0.8% sucrose, the agar of 1.6%, all the other are distilled water, regulate pH to be 7.0 after mixing, bevel medium, simple bacillus to be seeded is inoculated on described slant medium, be placed in 25 DEG C of constant temperature culture 2 days, obtain the test tube strains of bacterial strain.
Percentage by weight is being respectively the beef extract of 0.3%, the peptone of 1.2%, the yeast extract of 0.8%, 1.2% sucrose, all the other are distilled water, and it is the aqueous fermentation medium making bacterial strain after 7.0 that said components fully mixes rear adjustment pH.
Described simple Bacillus is inoculated in liquid simple fermentation of bacillus medium, at 28 DEG C, with rotating speed 180rmin
-1shaken cultivation 24h in shaking table, obtains the zymotic fluid of bacterial strain.
Embodiment 3
For controlling a biological seed coating agent for soybean root disease, comprise 84.7% there is the simple fermentation of bacillus liquid killing line activity, the wherein CuSO of 0.12%
4, (the NH of 0.25%
4)
6mo
7o
244H
2o, the H of 0.05%
3bO
3, the MnSO of 0.4%
4, the FeSO of 0.06%
4, the KH of 2.2%
2pO
4, the K of 1%
2sO
4, the calcium lignosulfonate of 5%, the methylcellulose of 2.8%, the peacock green of 1%, and by 0.12% xanthans and the binding agent that forms of 2.3% modified starch, above-mentioned each component is prepared by mass percentage.Concrete preparation process is: by the CuSO of above-mentioned 0.12%
4, (the NH of 0.25%
4)
6mo
7o
244H
2o, the H of 0.05%
3bO
3, the MnSO of 0.4%
4, the FeSO of 0.06%
4, the KH of 2.2%
2pO
4, the K of 1%
2sO
4grinding is pulverized after mixing, add the calcium lignosulfonate of 5% again, the methylcellulose of 2.8%, the peacock green of 1%, and by 0.12% xanthans and the binding agent that forms of 2.3% modified starch, and the mixed culture fermentation liquid of described three kinds of bacterial strains of 84.7% stirs into uniform viscous fluid, vibration mixes, and prepares described biological seed coating agent.Obtained biological seed coating agent and the ratio of grain weight are 5: 70, are evenly wrapped in soya seeds surface, can sow after drying.
Wherein, above-mentioned to have the bacterial strain fermentation liquor killing line activity be simple fermentation of bacillus liquid, and concrete preparation method is as follows:
Percentage by weight is respectively the beef extract of 0.4%, the peptone of 0.6%, the yeast extract of 0.2%, 1% sucrose, the agar of 1.8%, all the other are distilled water, regulate pH to be 7 after mixing, bevel medium, simple bacillus to be seeded is inoculated on described slant medium, be placed in 25 DEG C of constant temperature culture 2 days, obtain the test tube strains of bacterial strain.
Percentage by weight is being respectively the beef extract of 0.4%, the peptone of 1.2%, the yeast extract of 1.0%, 1.5% sucrose, all the other are distilled water, and it is the aqueous fermentation medium making bacterial strain after 7.0 that said components fully mixes rear adjustment pH.
Described simple Bacillus is inoculated in liquid simple fermentation of bacillus medium, at 25 DEG C, with rotating speed 200rmin
-1shaken cultivation 72h in shaking table, obtains the zymotic fluid of bacterial strain.
Embodiment 4:
To through implement soya seeds after 1 and the obtained biological seed coating agent process of embodiment 2 respectively in 2012 in Kangping County, Liaoning Province, Daqing, Heilongjiang Province carries out field plot sowing test.The method that small plot experiment takes random district group to repeat for 5 times.Each process 5 row district, row long 2.5m, line-spacing 0.65m, process interval 1m, plot area 8.1m
2, gross area 450m
2.Using the not soya seeds of dressing and the seed of commodity seed coat agent process as contrast.Wherein, commodity seed coat agent is that BFA continuous cropping is red, and production unit: Inner Mongol Mo Qi branch company of middle wealth flourishing age (Beijing) biotechnology Development Co., Ltd, main component is biological seed coating agent " BFA " 20%, standard code: Q/CHS03-2001.
The change of the outer cyst of table 1 soybean biological seed coating agent processed soybeans root and Gen Nei nematode population
Note: after numeral, letter is Duncan's multiple range test result, different capital and small letter English alphabet represents difference extremely significantly (p < 0.01) and significant difference (p < 0.05) respectively, and following table is same.
Experimental data as shown in Table 1 can be found out, the soya seeds of biological seed coating agent process prepared by the present embodiment 1 in Kangping, the soya seeds of the preventive effect of grand celebration and the not dressing of blank compares cyst inhibiting rate on root and improves 35.36% and 39.29% respectively, the soya seeds of biological seed coating agent process prepared by the present embodiment 2 in Kangping, the soya seeds of the preventive effect of grand celebration and the not dressing of blank compares cyst inhibiting rate on root and improves 49.7% and 54.2% respectively, significant difference; Also the significance level of difference is reached with the soya seeds of commodity seed coat agent process.The root interior lines borer population of the soya seeds of the biological seed coating agent process that the embodiment of the present invention 1 provides is compared with the soya seeds of the not dressing of blank, 60.93% and 40.2% are improve respectively to nematode inhibiting rate in root, and the root interior lines borer population of the soya seeds of the biological seed coating agent process that the embodiment of the present invention 2 provides is compared with the soya seeds of the not dressing of blank, 77.23% and 55.1% are improve respectively to nematode inhibiting rate in root.Simultaneously, the soya seeds of biological seed coating agent process provided by the invention significantly can also suppress the propagation of cyst quantity in soil, implement fall 1 compared with the soya seeds of the not dressing of blank difference reach pole significance level, inhibiting rate improves 36.60% and 37.66% respectively, embodiment 2 difference compared with the soya seeds of the not dressing of blank reaches pole significance level, and inhibiting rate improves 54.6% and 57.23% respectively.Because Kangping is different with the field plot incidence of grand celebration, nematode microspecies are different, so may cause result slightly difference.
Table 2 soybean biological seed coating agent is on the impact of plant strain growth
By data analysis shown in table 2, can see in seedling stage, biological seed coating agent prepared by the embodiment of the present invention 1 and embodiment 2 can promote the growth of soybean seedling safely and effectively; After the soya seeds nursery of the biological seed coating agent process adopting the embodiment of the present invention 1 and embodiment 2 to prepare to Kangping and grand celebration proving ground in the soybean mature phase, significantly promote the plant height of soybean, individual plant English number and single-strain grain number Isoquant constituent element.
Table 2 measurement data shows, and there were significant differences for Adult plant soybean plant height and not dressing blank, and there were significant differences compared with blank for single-strain legumen number; Individual plant English grain number is also higher than blank and the contrast of commodity seed coat agent.Illustrate that biological seed coating agent that the embodiment of the present invention 1 and embodiment 2 provide not only can be good at suppressing the generation of soybean root disease, but also the growth of soybean and the increase of 100-grain weight can be promoted, thus reach volume increase object.
In addition, within 2012, in Helen experiment station of Heilongjiang Province and Heihe experiment station, for blank, carry out field plot sowing test with not dressing, specific experiment data are as shown in table 3 below.
The change of table 3 soybean biological seed coating agent processed soybeans root rot
Note :-indicate without preventive effect
Biological seed coating agent provided by the invention in Helen experiment station to the preventive effect of root rot and soybean cyst nematode compared with blank, embodiment 1 pair of root rot and soybean cyst nematode inhibiting rate are respectively 46.15% and 70.72%, embodiment 2 pairs of root rots and soybean cyst nematode inhibiting rate are respectively 58.9% and 82.3%, all reach difference pole significance level.And biological seed coating agent provided by the invention can significantly improve output, the biological seed coating agent per mu yield that embodiment 1 provides improves 22.67% and 25.72% compared with the control respectively in Helen experiment station and Heihe experiment station, and the biological seed coating agent per mu yield that embodiment 2 provides improves 42.8% and 44.6% compared with the control respectively in Helen experiment station and Heihe experiment station.
Above result of the test shows, this biological seed coating agent has good preventive effect to soybean cyst nematode and root rot, and can significantly improve soybean yields, demonstrates good development prospect.
The above embodiment is only that its protection domain is not limited thereto in order to absolutely prove the preferred embodiment that the present invention lifts.The equivalent alternative or conversion that those skilled in the art do on basis of the present invention, all within protection scope of the present invention, protection scope of the present invention is as the criterion with claims.
Claims (10)
1. for controlling a biological seed coating agent for soybean root disease, it is characterized in that, comprise following component, each component is prepared by mass percentage:
The CuSO of 0.02% ~ 0.15%
4, (the NH of 0.05% ~ 0.26%
4)
6mo
7o
244H
2o, the H of 0.01% ~ 0.05%
3bO
3, the MnSO of 0.1% ~ 0.5%
4, the FeSO of 0.04% ~ 0.25%
4, the KH of 1.5% ~ 6%
2pO
4, the K of 0.02% ~ 1%
2sO
4, the dispersant of 2% ~ 8%, the film forming agent of 2% ~ 5%, the warning colouration of 0.1% ~ 1.2%, the binding agent of 0.95% ~ 2.65%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity.
2. the biological seed coating agent for controlling soybean root disease according to claim 1, is characterized in that, comprises following component, and each component is prepared by mass percentage:
The CuSO of 0.04%
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4, the K of 0.05%
2sO
4, the dispersant of 6%, the film forming agent of 4%, the warning colouration of 1%, the binding agent of 2.55%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity.
3. the biological seed coating agent for controlling soybean root disease according to claim 1 and 2, is characterized in that, described biocontrol bacterial strain zymotic fluid is simple fermentation of bacillus liquid.
4. the biological seed coating agent for controlling soybean root disease according to claim 3, is characterized in that, described simple bacillus is separated and obtains from soybean root system soil.
5. the biological seed coating agent for controlling soybean root disease according to claim 3, is characterized in that, described biocontrol bacterial strain zymotic fluid is by bacterial strain to be seeded after test tube strains is cultivated, then obtain through expansion fermented and cultured, concrete preparation method is as follows:
Percentage by weight is respectively the beef extract of 0.2% ~ 0.4%, the peptone of 0.4% ~ 0.6%, the yeast extract of 0.1% ~ 0.2%, 0.8% ~ 1.0% sucrose, the agar of 1.2% ~ 1.8%, all the other are distilled water, regulate pH to be 7 after mixing, bevel medium, simple Bacillus strain to be seeded is inoculated on described slant medium, be placed in 25 DEG C of constant temperature culture 2 days, obtain test tube strains;
Percentage by weight is respectively the beef extract of 0.2% ~ 0.4%, the peptone of 1% ~ 1.2%, the yeast extract of 0.5% ~ 1%, 1%-1.5% sucrose and excess water mixing, regulates pH to make liquid fermentation medium after 6.8 ~ 7 afterwards;
Described test tube strains is inoculated in described liquid fermentation medium, at 25 DEG C ~ 28 DEG C, with rotating speed 150rmin
-1~ 200rmin
-1shaken cultivation 24h ~ 72h in shaking table, obtains described biocontrol bacterial strain zymotic fluid.
6. the biological seed coating agent for controlling soybean root disease according to claim 1, is characterized in that, described dispersant is calcium lignosulfonate; Described film forming agent is methylcellulose; Described warning colouration is peacock green.
7. the biological seed coating agent for controlling soybean root disease according to claim 6, is characterized in that, described binding agent is mass percent is the xanthans of 1.5-20: 100 and the mixture of modified starch composition.
8. the preparation method of the biological seed coating agent for controlling soybean root disease according to claim 1, is characterized in that,
Percentage by weight is respectively the CuSO of 0.02% ~ 0.15%
4, (the NH of 0.05% ~ 0.26%
4)
6mo
7o
244H
2o, the H of 0.01% ~ 0.05%
3bO
3, the MnSO of 0.1% ~ 0.5%
4, the FeSO of 0.04% ~ 0.25%
4, the KH of 1.5% ~ 6%
2pO
4with 0.02% ~ 1.0% K
2sO
4grinding is pulverized after mixing, add the dispersant of 2% ~ 8% again, the film forming agent of 2% ~ 5%, the warning colouration of 0.1% ~ 1.2%, the binding agent of 0.95% ~ 2.65%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity, and said components stirs into uniform viscous fluid, vibration mixes, and prepares described biological seed coating agent.
9. the preparation method of the biological seed coating agent for controlling soybean root disease according to claim 8, is characterized in that,
The quality accounting of each component is the CuSO of 0.04%
4, (the NH of 0.08%
4)
6mo
7o
244H
2o, the H of 0.02%
3bO
3, the MnSO of 0.2%
4, the FeSO of 0.22%
4, the KH of 5%
2pO
4with 0.05% K
2sO
4pulverize grinding after mixing, then add the dispersant of 6%, the film forming agent of 4%, the warning colouration of 1%, the binding agent of 2.55%, all the other are have the biocontrol bacterial strain zymotic fluid killing line activity.
10. the biological seed coating agent for controlling soybean root disease according to claim 1 and 2, it is characterized in that, for soya seeds coated to be sowed, during use, by mass percent be 1-5: 70 biological seed coating agent mix with soya seeds, make biological seed coating agent evenly be coated on soya seeds surface, can sow after drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511009463.5A CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511009463.5A CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105494447A true CN105494447A (en) | 2016-04-20 |
| CN105494447B CN105494447B (en) | 2018-11-02 |
Family
ID=55703060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511009463.5A Active CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105494447B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818188A (en) * | 2017-03-03 | 2017-06-13 | 河南科技学院 | A kind of good quality and high output abridged edition protects excellent wheat cultivating method |
| CN106986719A (en) * | 2016-10-26 | 2017-07-28 | 沈阳农业大学 | And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode |
| CN109923975A (en) * | 2019-03-21 | 2019-06-25 | 陆福 | A kind of implantation methods of semen sojae atricolor kind |
| NL2020261B1 (en) * | 2018-01-10 | 2019-07-15 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Method for treating plant seed |
| CN110218665A (en) * | 2019-05-13 | 2019-09-10 | 沈阳农业大学 | One plant of bacillus and application with the anti-leaf blight of corn effect of inducing maize |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
-
2015
- 2015-12-23 CN CN201511009463.5A patent/CN105494447B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
Non-Patent Citations (1)
| Title |
|---|
| 《中国生物防治学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986719A (en) * | 2016-10-26 | 2017-07-28 | 沈阳农业大学 | And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode |
| CN106818188A (en) * | 2017-03-03 | 2017-06-13 | 河南科技学院 | A kind of good quality and high output abridged edition protects excellent wheat cultivating method |
| NL2020261B1 (en) * | 2018-01-10 | 2019-07-15 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Method for treating plant seed |
| CN109923975A (en) * | 2019-03-21 | 2019-06-25 | 陆福 | A kind of implantation methods of semen sojae atricolor kind |
| CN110218665A (en) * | 2019-05-13 | 2019-09-10 | 沈阳农业大学 | One plant of bacillus and application with the anti-leaf blight of corn effect of inducing maize |
| CN110218665B (en) * | 2019-05-13 | 2021-12-14 | 沈阳农业大学 | Bacillus with corn leaf blight resistance inducing effect and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105494447B (en) | 2018-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105724438B (en) | A kind of functional microbial inoculum for preventing and treating root-knot nematode | |
| CN105494447B (en) | Biological seed coating agent and its preparation method and application for controlling soybean root disease | |
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| Shariatmadari et al. | Study of soil blue-green algae and their effect on seed germination and plant growth of vegetable crops | |
| CN108034620B (en) | One plant of bacillus megaterium bacterial strain, peanut drop cadmium agent and its application | |
| CN107266251B (en) | Soil nutrient activator for saline-alkali soil and preparation method thereof | |
| JP2017532062A (en) | Microbial homogeneous extract using useful and functional microorganisms and method for producing the same | |
| CN102851245B (en) | Tobacco endophyte strain inhibiting tobacco bacterial wilt, and microbial agent thereof | |
| CN112970780A (en) | Special microbial agent for ginseng and method for reducing incidence rate of ginseng rust rot | |
| CN103387428A (en) | Preparation method for organic material decomposition agent | |
| CN109055274B (en) | Caragana rhizobium and fermentation culture method and application thereof | |
| CN104450560A (en) | Nematode-killing sphingosine bacillus strain and application thereof | |
| CN109749940B (en) | Preparation method of fungal inoculant, product and application thereof | |
| CN101717646A (en) | Soil conditioner for preventing and curing wheat take-all in newly cultivated farmland, and preparation method and using method thereof | |
| CN103964928A (en) | Preparation method of microorganism soil restoration agent | |
| CN104774788B (en) | Lawn salt tolerant strengthens the preparation method and application of complex microbial community in garbage compost | |
| CN103667083B (en) | A kind of mould, its cultural method of top spore and the application in preparation original spore powder of meloidogyne pesticide thereof | |
| CN110679611A (en) | Application of fusarium proliferatum in prevention and control of invasive plant xanthium sibiricum | |
| CN102648714A (en) | Method for preparing biopesticide through liquid fermentation | |
| CN101818124B (en) | Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof | |
| CN103937729A (en) | Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent | |
| CN104193549A (en) | Biological additive for promoting alfalfa green manure to increase crop yield | |
| CN102379315A (en) | Directional regulating agent of rhizosphere microorganism and nematode containing saturated fatty acid | |
| CN114920593A (en) | Biological compound organic fertilizer and preparation method thereof | |
| CN103849592A (en) | Production method for streptomycete spores |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |