CN102925387A - Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application - Google Patents
Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application Download PDFInfo
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Abstract
The invention relates to a method for culturing a Bacillus simplex Sneb545 for inducing soybean to generate soybean cyst nematode resistance after soybean seed treatment and an application of the Bacillus simplex Sneb545, and belong to the technical field of microbial pesticides. The bacterial strain Sneb545 related in the invention is preserved in the China general microbiological culture collection center in October 11th, 2012, and the preservation number is CGMCC No.6650. After the strain is subjected to fermentation cultivation through conventional liquid, the metabolic products of the strain can be used to develop a seed treating agent for treating soybean seeds, and the treated soybean seeds can induce the soybeans to generate the soybean cyst nematode resistance. The soybean seeds which is treated through the metabolic products of the Bacillus simplex Sneb545 can induce the soybeans to generate obvious resistance to the first generation of soybean cyst nematode during the seedling stage, the Bacillus simplex Sneb545 has an obvious function of inhibiting the cytocyst of the soybean cyst nematode from forming and is a potential biocontrol bacterium, and a new method is provided for preventing the soybean cyst nematode.
Description
Technical field
The invention belongs to the microbial pesticide technical field, be specifically related to simple genus bacillus and the application of a kind of tool inducing soybean Chinese People's Anti-Japanese Military and Political College beans Cyst nematode.
Background technology
Soybean cyst nematode (Soybean Cyst Nematode, be called for short SCN) be by soybean cyst nematode Heterodera glycines (
Heterodera glycines) infect and cause, be one of the most serious disease of harm world soybean production.This disease has wide, the characteristics such as harm is heavy, host range is wide, the route of transmission is many, hypopus (cyst) survival time length that distribute, and is a kind of soil-borne disease of extremely difficult control.In recent years along with the continuous expansion of China's soybean culture area, the distribution of soybean cyst nematode Heterodera glycines is also more and more wider, mainly occurs in totally 14 provinces, cities and autonomous regions such as northeast and Huang-Huai-Hai, and soybean is subjected to its rear general underproduction 20%～30% of causing harm, severe one can reach 70%～80%, even No kernels or seeds are gathered, as in a year of scarcity.Soybean cyst nematode Heterodera glycines is the settling down property endoparasitism nematode that soil passes, and infects plant root, becomes the main bugbear in the Soybean production of present countries in the world, and most of nematode killing agent serious environment pollutions that chemical prevention is used destroy the eubiosis.Over nearly 20 years, along with the development of sustainable agriculture and Organic farming, the focus that people will prevent and treat nematodiasiss has turned to biological control.
The biological and ecological methods to prevent plant disease, pests, and erosion factor of soybean cyst nematode Heterodera glycines has a lot, comprises fungi, bacterium, virus and protozoon etc.Utilize plant rhizosphere to urge the existing research of living bacterium (plant growth promoting rhizobacteria, PGPR) control soybean cyst nematode evil, Becker etc. reported in 1988 Rhodopseudomonas (
Pseudomonas) and bacillus (
Bacillus) the root knot nematode disease there is preventive and therapeutic effect.Racke and Sikora reported rhizosphere bacteria in 1992
Bacillus sp. and
B.cereusTo the control Meloidogyne incognita (
Meloidogyne incognita), soybean cyst nematode Heterodera glycines (
Heterodera glycines), the corn Cyst nematode (
Heterodera zeae) and Heterodera avenae (
Heterodera avenae) larva is effective.Bacillus (
Bacillus sp.) bacterium can produce multiple antimicrobial substance, comprise lipopeptid class, peptide class, phospholipid, bacteriophage-like particle and bacteriocin etc., have the function of antibacterium, fungi, virus and mycoplasma.And genus bacillus is easy to breeding, and accommodative ability of environment is strong; Most genus bacillus are nontoxic to people and animals, have very strong environmental compatibility and environment friendly, thereby play an important role in biological control, have very large value of exploiting and utilizing.Correlative study shows that simple genus bacillus has short giving birth to and Biocontrol Effect, and it up to 83%, increases Chinese Cabbage Yield 50% for the control rate of soft rot of Chinese cabbage simultaneously, and simple genus bacillus also not yet has report to the control of soybean cyst nematode.
For producing upper this problem that exists, obtain a strain bacterium by screening system, utilize the meta-bolites processed soybeans seed of this bacterium or this bacterium, but inducing soybean produces the resistance to soybean cyst nematode Heterodera glycines during Seed Germination and growth, thereby solved the problem of soybean cyst nematode.
Summary of the invention
Can induce Soybean Seedling that Cyst nematode is produced obvious resistance after the objective of the invention is to select a strain bacterial strain that soybean seeds is processed, with bacterial strain routinely after the liquid fermentation and culture, can be with the meta-bolites of this bacterium, develop the seed treatment agent of processed soybeans seed, treated soybean seeds can inducing soybean produces the resistance to soybean cyst nematode Heterodera glycines.
The strain bacterial isolates that the present invention screens acquisition be simple genus bacillus (
Bacillus simplex) Sneb545, depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: No. 1 Institute of Microorganism, Academia Sinica in BeiChen West Road, Chaoyang District, BeiJing City; Preservation date: on October 11st, 2012; The numbering that preservation is registered on the books: CGMCC No.6650.Simple genus bacillus (
Bacillus simplex) Sneb545 is the strain that filters out from Chinese Shenyang City, Liaoning Province Agricultural University Of Shenyang experimental plot has strong induced activity to soybean bacterial isolates.
The present invention is by the research discovery, and soil bacteria has a lot of strain bacteriums all to have the ability of certain anti-Cyst nematode of inducing soybean, filters out relatively preferably bacterial strain of a strain proterties from these bacteriums---simple genus bacillus (
Bacillus simplex) Sneb545 carried out further research.
The invention still further relates to the bacterium metabolite that produces anti-Cyst nematode resistance for inducing soybean, its be by the simple genus bacillus of bacterial isolates of the present invention (
Bacillus simplex) Sneb545 obtains after through conventional liquid fermentation and culture.
The simple genus bacillus of bacterial isolates of the present invention (
Bacillus simplex) Sneb545 is after cultivating by culture dish, prepares through enlarging fermentation culture again, its concrete grammar is as follows:
1. culture dish is cultivated
Culture medium prescription is beef extract-peptone nutrient agar (NA): beef extract 3g, peptone 5g, sucrose 10g, agar 17-20g, distilled water 1000mL.
2. liquid fermentation and culture
Wherein the liquid fermentation medium prescription is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3%-0.4%, sucrose 1%-2%, remainder is distilled water, pH is 6.8-7.0.
In 250mL triangular flask (the every bottled 100mL) liquid nutrient medium, under 25 ℃～28 ℃, shaking speed is with 150r min with bacterial classification inoculation
-1～180rmin
-1, fermentation 2-3d, preferred 3d.
The bacterial classification of liquid fermenting can carry out scale production by large fermentation tank, and its product is the finished product of fermented liquid or fermented liquid.
The invention still further relates to a kind of soybean seeds treatment agent, its comprise significant quantity of the present invention simple genus bacillus (
Bacillus simplex) Sneb545 itself or its metabolite be as the effective active composition.
Simple genus bacillus of the present invention (
Bacillus simplex) Sneb545 or its metabolite have the induced activity to soybean, can be used for the seed treatment of soybean, the soybean after the processing forms the cyst of soybean cyst nematode Heterodera glycines has good restraining effect, has solved the difficult problem of soybean cyst nematode.
Embodiment:
In order further to illustrate in more detail rather than in order to limit the present invention, to have provided the following example.
Embodiment 1: simple genus bacillus (
Bacillus simplex) cultivation of Sneb545
At first to simple genus bacillus (
Bacillus simplex) the Sneb545 bacterial strain carries out fermentation culture, then the meta-bolites after the fermentation culture carried out the test of soybean induced activity, determines that it is to the inducing action of soybean.
With simple genus bacillus (
Bacillus simplex) thalline of Sneb545 is inoculated on the substratum, culture medium prescription is beef-protein medium, i.e. composition: beef extract 3g, peptone 5g, sucrose 10g, agar 17-20g, adding distil water cultivate 2d for 25 ℃ to 1000mL.
Again bacterial classification inoculation is arrived in 250mL triangular flask (the every bottled 100mL) liquid nutrient medium, culture medium prescription is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3%-0.4%, sucrose 1%-2%, remainder is distilled water, pH is 6.8-7.0, leavening temperature is 25-28 ℃, shaking speed 150rmin
-1～180rmin
-1, fermentation time 2-3d.The fermented liquid that forms or the finished product of fermented liquid are the processed soybeans seed directly or indirectly.Processed soybean seeds can alleviate because the harm that the soybean cyst nematode evil causes.
Embodiment 2: simple genus bacillus (
Bacillus simplex) fermentation of Sneb545
Substantially with embodiment 1, difference is that the bacterial classification inoculation of the liquid culture fermentor tank to 1000L is fermented, and 25 ℃ of lower cultivations, pH is 7.0, fermentation 3d.
Embodiment 3: simple genus bacillus (
Bacillus simplex) checking of Sneb545 inducing soybean system resistance
Utilization is split root test design (split-root system) and has been inquired into the inducible system resistance of bacterial strain Sneb545 to soybean cyst nematode Heterodera glycines.Split the challenge root system in the root test and reply each other spatially fully isolation of root system, reply Sneb545 fermented liquid and soybean cyst nematode Heterodera glycines in the root system directly contacting, bacterial strain is to the control action kou of nematode its induction of resistance to plant that places one's entire reliance upon.This measuring method is reliable, has been widely used in Biocontrol microorganism to the system induction resistance research of soil-borne disease.
1. preparation is tested and is used preparation
The fermented liquid for preparing simple Bacillus strain Sneb545 by the aforementioned liquids fermentation culture method is treated for subsequent use.
2. nematode is used in the preparation test
Soybean cyst nematode Heterodera glycines (
Heterodera glycines): No. 3 physiological strains of soybean cyst nematode Heterodera glycines are taken from north nematode institute of Agricultural University Of Shenyang test base.Adopt improvement to eluriate-cross sieve method and from the soil sample of taking, separate cyst, the cyst of fresh, full, ripe, the homogeneous of picking under stereoscope.Cyst with the 0.5% chlorine bleach liquor 3min that sterilizes, is used aseptic water washing 5 times first again, is placed in the culture dish that contains a small amount of sterilized water, cultivates in 25 ℃ of thermostat containers, and the tray hatching obtained 2 instar larvaes after 15 days, and 2 instar larvae suspension are adjusted to 200/ml.
3. preparation is tested and is used seed
The Liao Dynasty's beans 15: provided by Liaoning Province Academy of Agricultural Sciences.
4. method for treating seeds
Soybean seeds is used first 5% chlorine bleach liquor's surface sterilization 5min, use again aseptic water washing 5 times.
5. test method
The employing greenhouse pot culture is processed, and gets without the soil of soybean cyst nematode Heterodera glycines and husky, and is in the dry sterilization pot 165 ℃, the 2h sterilization, then husky take V (soil): V() as during the ratio mixing of 2:1 packs 16 * 16cm polypots into.3 distant beans 15 seeds of sowing in every alms bowl, when treating that bean seedlings grow to two cotyledons, every alms bowl stays 1 strain.Bean seedlings are taken out and clean, and root system is divided into impartial two portions.Get 3 onesize polypotss, become " product " font to place, the polypots of bottom is put in respectively in the pan arrest of 2 16cm diameters.2 1cm * 2cm are opened in polypots bottom at the top, seedling places the polypots at top, root system is separately arrived in the soil of two polypotss of bottom by 2 cracks respectively, the root system of growing in two polypotss in bottom is respectively the challenge root system and replys root system, fixedly plant of the soil matrix of packing in 3 alms bowls.System places greenhouse (25 ℃, 14h illumination), the 10ml of inoculation Sneb545 fermented liquid below the 2cm table soil in the polypots of challenge root system, and inoculation 10ml J2 suspension is to replying root system after 1 week of inoculation Sneb545 fermented liquid.Take sterilized water as contrast, totally 2 processing, 3 repetitions.The 35d " Invest, Then Investigate " is replied cyst amount and the sum of the soybean cyst nematode Heterodera glycines in the root in the soil in the root system alms bowl.
5.1 the separation of cyst and counting in the soil: each soil sample takes by weighing 200cc (namely with the 1000mL beaker 800mL that is filled with water, adding soil to 1000mL), adopt eluriate-to sieve-the graceful funnel method of shellfish collects cyst, the quantity of record cyst under stereoscopic anatomical lens.
5.2 dyeing and the counting of nematode in the root: root system is cleaned up with flowing water, claim the root fresh weight, then use clorox-acid fuchsin staining to carry out the dyeing of nematode in the root, nematode population in the observed and recorded root under stereoscopic microscope, and be converted into the quantity of every gram root fresh weight nematode.
5.3 prevention effect is calculated
Cyst inhibiting rate (%)=
Nematode decrement (%)=
6 test-results
Measure demonstration, Sneb545 can produce the resistance very strong to soybean cyst nematode Heterodera glycines by inducing soybean.Bacterial strain Sneb545 after the inoculation, replys that the invasion of soybean cyst nematode Heterodera glycines significantly reduces in the root system in the challenge root system, reply that cyst quantity significantly reduces in the Soil-in-Root.Reply the invasion of root system soybean cyst nematode Heterodera glycines and reduce by 51.27%, cyst reduces 65.82%(table 1 in the soil).
Reply behind the table 1 Sneb545 inoculation challenge root system and invade cyst sum in nematode and the soil in the root system
| Process | Cyst quantity in the soil | Soybean cyst nematode Heterodera glycines quantity in the root |
| Sneb545 | 9.0000±2.6458bB | 32.0000±4.3589bB |
| CK | 26.3333±2.6034aA | 65.6667±5.6075aA |
Annotate: the investigation result behind 35 d, letter is Duncan ' s multiple range test result after the numeral, different capital and small letters are English
Letter represents respectively difference extremely significantly (P＜0. 01) and significant difference (P＜0. 05).
Embodiment 4: simple genus bacillus (
Bacillus simplex) the field control soybean cyst nematode effect test of Sneb545
Use fermented liquid stoste, ratio treatment coatings soybean seeds in 1:100, after drying, the soybean seeds of processing is normally sowed on soybean cyst nematode piece ground, through inducing Soybean Seedling that Cyst nematode is produced obvious resistance behind the Sneb545 metabolite processed soybeans seed, to the obvious restraining effect of being formed with of soybean cyst nematode Heterodera glycines cyst, be very potential biocontrol microorganisms, for the control soybean cyst nematode has been opened up new approaches.
1. preparation is tested and is used preparation
The fermented liquid for preparing simple Bacillus strain Sneb545 by the aforementioned liquids fermentation culture method is treated for subsequent use.
2. preparation is tested and is used seed
The Liao Dynasty's beans 15: provided by Liaoning Province Academy of Agricultural Sciences.
Close rich 50: provided by grand celebration branch of Heilongjiang Institute of Agricultural Sciences.
3. method for treating seeds
Soybean seeds is used first 5% chlorine bleach liquor's surface sterilization 5min, use again aseptic water washing 5 times.Adopt 1% grain weight to carry out seed pelleting the fermented liquid for preparing and process, rear for subsequent use wait drying.
4. test method
Adopt field natural occurrence soil inoculation soybean.Carry out revision test 5 times in Kangping County, Liaoning Province and two places, Daqing, Heilongjiang Province respectively.Soybean Field is the secondary soybean field, test base, Kangping continuous cropping 3 years, and field cyst amount is 40 cysts/100ml soil, and grand celebration test base continuous cropping is more than 6 years, and field cyst amount is 55 cysts/100ml soil.The field Quarter Design is 3 processing, repeats for 5 times, and totally 15 residential quarters, random district group is arranged, 5 ridges, every residential quarter, long 5 meters of ridge, 1 meter in passageway, 100 of every ridge plantings, cell edge is established guard rows.Take not through the soybean seeds of the soybean seeds of dressing and NA liquid medium dressing as contrast, 35d behind soybean planting, after soybean cyst nematode Heterodera glycines grows the breakthrough soybean rhizoplane, each residential quarter is got 20 young plants at random, after removing table soil (0-5cm) plant is grubbed out out, keep the complete of root system, investigation individual plant soybean root system cyst the amount of giving birth to, and calculates the cyst inhibiting rate.
5. test-results
Soybean to the processing of Sneb545 bacterial strain, the situation of giving birth at the aobvious capsule phase investigation of soybean cyst nematode Heterodera glycines root system cyst, large bean seedlings after the Sneb545 bacterial isolates Cotton seeds have formed obvious restraining effect to the cyst of soybean cyst nematode Heterodera glycines, as shown in Table 2, Sneb545 has reached respectively 57.67%, 47.92% at the inhibiting rate of large Khanh Hoa Kangping, soybean after the Cotton seeds has different in the result of Kangping and the processing of grand celebration two places, and this may be relevant with the difference of different areas Soybean Cyst Nematode.
The impact that table 2 Sneb545 bacterial strain Cotton seeds soybean seeds forms the soybean root system cyst
| Process | The Heilungkiang grand celebration | Kangping, Liaoning |
| Sneb545 | 39.2±3.6387bBC | 45.35±4.7315bcdB |
| NA | 93.2±5.0735aA | 84.34±5.8726aA |
| CK | 92.6±3.7762aA | 87.08±8.1215aA |
Annotate: the investigation result behind 35 d, letter is Duncan ' s multiple range test result after the numeral, different capital and small letters are English
Letter represents respectively difference extremely significantly (P＜0. 01) and significant difference (P＜0. 05).
Claims (7)
1. the bacterial isolates that has inducing soybean Chinese People's Anti-Japanese Military and Political College beans Cyst nematode behind the strain processed soybeans seed is characterized in that: this bacterial strain be simple genus bacillus (
Bacillus simplex), being deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on October 11st, 2012, preserving number is CGMCC No.6650.
2. the liquid fermentation process of bacterial isolates claimed in claim 1, it is characterized in that: the liquid fermentation medium prescription is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3%-0.4%, sucrose 1%-2%, remainder is distilled water, pH is 6.8-7.0, leavening temperature is 25-28 ℃, shaking speed 150rmin
-1～180rmin
-1, fermentation time 2-3d.
3. liquid fermentation process according to claim 2, it is characterized in that: described fermentation time is 3d.
4. has the preparation method that inducing soybean produces the fermented liquid stoste of Chinese People's Anti-Japanese Military and Political College's beans Cyst nematode resistance, it is characterized in that: formed through behind the liquid fermenting by bacterial isolates claimed in claim 1, the liquid fermentation medium prescription is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3%-0.4%, sucrose 1%-2%, remainder is distilled water, pH is 6.8-7.0, and leavening temperature is 25-28 ℃, shaking speed 150rmin
-1～180rmin
-1, fermentation time 2-3d.
5. preparation method according to claim 4, it is characterized in that: described fermentation time is 3d.
6. a soybean seeds treatment agent is characterized in that, comprises formed fermented liquid stoste in the liquid fermentation process according to claim 2 of significant quantity, is used for inducing soybean and produces resistance to soybean cyst nematode Heterodera glycines.
7. the application of fermented liquid stoste in inducing soybean Chinese People's Anti-Japanese Military and Political College beans Cyst nematode that forms in the described liquid fermentation process of claim 2.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105002121A (en) * | 2015-08-14 | 2015-10-28 | 山东泰诺药业有限公司 | Bacillus simplex and application thereof |
| CN105112340A (en) * | 2015-09-15 | 2015-12-02 | 沈阳农业大学 | Fungus strain with induced resistance as well as culture method and application of fungus strain |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
| CN105695376A (en) * | 2016-04-25 | 2016-06-22 | 北京市农林科学院 | Bacillus simplex for poisoning plant root-knot nematodes and application of bacillus simplex |
| CN106986719A (en) * | 2016-10-26 | 2017-07-28 | 沈阳农业大学 | And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode |
| CN110275001A (en) * | 2019-07-31 | 2019-09-24 | 四川省农业科学院生物技术核技术研究所 | It is a kind of to measure and evaluate medicament to the apparatus and method of root-knot nematode drug effect |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102108339A (en) * | 2010-11-26 | 2011-06-29 | 沈阳农业大学 | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof |
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
-
2012
- 2012-10-26 CN CN201210414207.4A patent/CN102925387B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102108339A (en) * | 2010-11-26 | 2011-06-29 | 沈阳农业大学 | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof |
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
Non-Patent Citations (4)
| Title |
|---|
| A.V. STURZ & J. KIMPINSKI: "Endoroot bacteria derived from marigolds (Tagetes spp.) can decrease soil population densities of root-lesion nematodes in the potato root zone", 《PLANT AND SOIL》, 31 December 2004 (2004-12-31) * |
| JOSEPH W. KLOEPPER ET AL.: "Rhizosphere bacteria antagonistic to soybean cyst (Heterodera glycines) and root-knot (Meloidogyne incognita) nematodes: Identification by fatty acid analysis and frequency of biological control activity", 《PLANT AND SOIL》, 31 December 1992 (1992-12-31) * |
| 孙华 等: "大豆根际促生菌Sneb207对不同种类线虫毒性的研究", 《大豆科学》, vol. 28, no. 4, 30 August 2009 (2009-08-30) * |
| 段玉玺 等: "防治大豆胞囊线虫芽孢杆菌初步筛选", 《大豆科学》, vol. 27, no. 5, 31 October 2008 (2008-10-31) * |
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| CN105002121A (en) * | 2015-08-14 | 2015-10-28 | 山东泰诺药业有限公司 | Bacillus simplex and application thereof |
| CN105112340A (en) * | 2015-09-15 | 2015-12-02 | 沈阳农业大学 | Fungus strain with induced resistance as well as culture method and application of fungus strain |
| CN105112340B (en) * | 2015-09-15 | 2018-08-28 | 沈阳农业大学 | One kind having the active bacterium bacterial strain of induction of resistance and its cultural method and application |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
| CN105494447B (en) * | 2015-12-23 | 2018-11-02 | 沈阳农业大学 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
| CN105695376A (en) * | 2016-04-25 | 2016-06-22 | 北京市农林科学院 | Bacillus simplex for poisoning plant root-knot nematodes and application of bacillus simplex |
| CN105695376B (en) * | 2016-04-25 | 2019-03-19 | 北京市农林科学院 | One plant of simple bacillus for killing plant root-knot nematode and its application |
| CN106986719A (en) * | 2016-10-26 | 2017-07-28 | 沈阳农业大学 | And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode |
| CN110275001A (en) * | 2019-07-31 | 2019-09-24 | 四川省农业科学院生物技术核技术研究所 | It is a kind of to measure and evaluate medicament to the apparatus and method of root-knot nematode drug effect |
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